F. Morgan, J.-J. Maugas
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1 Whey, the natural source for a broad range of food ingredients F. Morgan, J.-J. Maugas LACTALIS R&D
2 Whey Skim milk Milk Casein production Cheese production Acid Rennet Fresh cheeses Soft cheeses Semi-hard cheeses Hard cheese Acid Whey Sweet Acid Whey Sweet
3 What can you obtain from whey? Whey powder Demineralised whey WPC 35 WPC80 and WPI Whey permeate Lactose and derivatives Calcium Native and denatured whey proteins Fractions with enriched proteins Hydrolysates Bioactive peptides Growth factors Oligosaccharides
4 Transform liquid into powder Before the 60 s, whey was either rejected or used for feed s: development of spray-drying technology ŁWHEY POWDER, feed and food grade Before drying the whey, lactose should be crystallised
5 Demineralised whey (60 s) Demineralised whey = the first component of an infant formula (40 to 50 % of the final product weight) Minerals in infant formulas should be low in order to comply to nutritional and regulatory needs Technologies for demineralization: 4Ion exchange 4Nanofiltration 4Electrodialysis ŁMore often a combination of 2 or 3 of these technologies
6 Demineralised whey From F. Gula (Novasep) 9 th Dairy Ingredients Symposium San Francisco 2007
7 Demineralised whey From F. Gula (Novasep) 9 th Dairy Ingredients Symposium San Francisco 2007
8 Ultrafiltration (60 s and 70 s) A real breakthrough was achieved at the end of the 60 s with the development of membrane filtration, thanks to Jean Louis Maubois and others.
9 WPC 35 (70 s) WHEY ULTRAFILTRATION (vol. conc. factor = 8) RETENTATE = WPC 35 Whey protein concentrate 35% Protein on dry matter PERMEATE (lactose and minerals) Opens new avenues for the valorisation of functional properties of whey proteins Ł Water holding Ł Gel forming properties Ł Emulsifying capacity
10 WPC80 an WPI (80 s) Obtained by Ultrafiltration / Diafiltration / Chromatography Higher concentration of protein together with lipid removal => improvement of technological properties Ł Higher gelling properties Ł Stabilisation of Foams High nutritional value => nutrition and diet market Ł Functional beverages (clear beverages; shakes) Ł Nutritional bars
11 Biological value of whey proteins Biological value Whey Egg Milk Beef Casein Soy Wheat
12 Native soluble milk proteins (90 s) Soluble milk proteins can be separated from milk by microfiltration (technology started in the 90 s) The soluble phase of milk contains all the native whey proteins, without GlycoMacroPeptide (only present in cheese whey) Benefits of soluble whey proteins ŁIncreased nutritional value for infant nutrition ŁBetter control on compositon and functional properties thereof
13 Denatured whey proteins Denaturation of whey proteins is caused by heat In order to control the structural state of denatured whey proteins, ph and calcium are the 2 main parameters Ł Structural Design is possible Denatured whey proteins have remarkable functional properties Ł Increased water retention (i.e. for fresh dairy products) for soluble whey protein aggregates Ł Heat stability for micellar whey protein Ł Creamy texture for reduced fat products is obtained through microparticulated whey proteins
14 Fractions with enriched proteins Alpha-Lactalbumin: the protein of human milk Production of bovine a-lactalbumin rich fraction Soluble milk proteins (milk microfiltrate) Controlled heat denaturation of β-lactoglobulin Microfiltration Permeate = native a-lactabumin Retentate = microparticulated β lactoglobumin Other proteins: lactoferrin, lactoperoxydase Ł Production by membrane processing and/or ionexchange chromatography Ł Bioactivity = anti-microbial properties, iron-binding (Lf)
15 Hydrolysates Hydrolysates are obtained by enzyme technology, sometimes combined with a fractionation technology (UF or chromatography) Hydrolysates can be divided in 2 main categories: ŁHypo-allergenic and non-allergenic hydrolysates ŁSpecific hydrolysates with bioactivity: 4With anti-oxidant properties (enriched with Cysteine) 4With anti-hypertensive effect 4.
16 Non-allergenic hydrolysate 2-7 % of infants are allergic to milk proteins => Diet = non-allergenic formulas with fully hydrolysed proteins NH2 L I V T Q T M K G L D I Q K S F N P T Q L E E QC H I L COOH R M P H A E K T K I I K Q K A E W C E G N I L A V A G T W Y K L A K D P E Q E S A L S A C N Q C L V R S L A M A A S D I S L I E P M SH A C K V F Y F L L I K D K Y A D L N T E N K K Q L L V L V L D I E L D G E P T P K L Example: tryptic peptides from b-lactoglobulin F K E L A E D D V T P E E V Y V E L D A Q S A P L R A combination of heat denaturation and enzymatic hydrolysis is needed since native whey proteins are resistant to enzymes. An ultrafiltration step might be added to remove bigger fragments with residual antigenicity
17 Bioactive whey peptides Peptides Lactorphins ACE inhibitory peptides Lactoferricin Protein precursor α-lactalbumin β-lactoglobulin α-lactalbumin β-lactoglobulin Lactoferrin Bioactivity Opioïd agonist Anti-hypertensive Anti-microbial
18 Growth factors Can be used in therapeutic products for wound healing and treatment of inflammatory gut disorders ŁIGF ŁTGF-b ŁEGF Isolation of growth factors from milk and colostrum whey using cation exchange chromatography and / or microfiltration / ultrafiltration From Pouliot and Gauthier (2006) Int. Dairy J., 16,
19 Prebiotics derived from whey Obtained by enzymatic or chemical conversion of lactose Galacto-oligosaccharides (GOS) Ł produced by lactose hydrolysis and transfer of the galactose on residual lactose by transgalactosylation Ł incorporated in infant milk formula as a bifidogenic factor in order to stimulate the growth of bifidobacteria Lactulose Ł produced by chemical or enzymatic isomerization of lactose Ł mainly used as a medicine for the treatment of chronic constipation and portal systemic encephalopathy Ł prebiotic effect
20 Whey oligosaccharides Health benefit: protection ŁNaturally-occurring prebiotic in milk ŁTrick harmful microbes, viruses and toxins (decoy function) Main oligosaccharides in bovine whey = Sialylated-oligosaccharides 4Concentration in cow s milk: 4Concentration in human milk: 0,1 g/l 1-2 g/l Technological challenge: development of enrichment strategies for large scale applications
21 Conclusion During the last 40 years, each technology pushes has led to new ingredients with improved functionalities Texture forming properties of whey proteins 4Whey is a toolbox for structure and texture design Nutrition and health: ŁExisting ingredients have a high nutritional value and bioactive properties ŁContinuous discovery of new health benefits 4 Whey is a toolbox for Nutrition
22 Future trends Emerging bioactive whey ingredients: oligosaccharides, bioactive peptides, Physical processing Ł Controlled heat induced interactions between whey proteins and other food components: lipid membranes, casein micelles.. Ł Processing with a lower thermal impact : ultra high pressure, cold processing.. Convergence of structure and nutrition: Impact of the structural state of whey ingredients on post-prandial metabolism? Ł Fast and slow proteins Ł Satiety induction..
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