Revolution in Photo-Protection, Lightening and Evening of Skin Tone Beauty from Within; a NutriCosmetics Innovation
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1 Patented technology by IBR Ltd. Revolution in Photo-Protection, Lightening and Evening of Skin Tone Beauty from Within; a NutriCosmetics Innovation Non-novel food (FSA) A natural powder from IBR proprietary, non-gmo tomato rich in phytoene and phytofluene
2 ABSTRACT PhytoflORAL, a natural dietary supplement for beauty from within comprising phytoene and phytofluene, the colorless carotenoids. The new clinical data shows that, PhytoflORAL has an outstanding photo-protection capacity that is built into the skin from within. PhytoflORAL can significantly increase MED (Minimal Erythemal Dose) after 84 days of consequent dietary consumption, meaning that the skin's ability to resist sun damage is increased. PhytoflORAL contains phytoene and phytofluene, colorless carotenoids also known to lighten and even the skin tone by inhibition of melanin synthesis, UVA and UVB protection, anti inflammatory and anti oxidant capabilities. The various effects working in concert, provide a state of the art product for (photo)protection and lighter, more even-toned skin. The consumer gains multi level benefits for healthier, well protected and younger looking skin all in one, natural and unique product - PhytoflORAL. BENFITS & USES Sun protection - Anti aging and photo aging Skin whitening/lightening & evening of skin tone Protection and boost of biomolecules Beauty from Within
3 PhytoflORAL Skin whitening / Lightening / Evening skin tone IBR-Phyto(flu)ene can block triggers of melanin synthesis IBR-Phyto(flu)ene can inhibit melanin synthesis Receptor NO, Free radicals UV Colorless carotenoids can: Inhibit melanin synthesis Activated Tyrosinase Tyrosinase Colorless carotenoids can: Absorb UV radiation Act as antioxidants Reduce the inflammatory response Tyrosine DOPAQuinone Eumelanins Melanosomes PGE, IL-1, IL-6 Inflammation Wound Eumelanins Melanosomes In vivo - topical: A randomized, double blinded, placebo controlled study evaluating the lightening effect of a cream containing IBR-TCLC 5% was performed. A twice a day application during a 3 month course of treatment resulted in a significant skin whitening effect at both measured time point of 42 days and 84 days (n=21) Variations in % +14% +12% +10% +8% +6% +4% +2% +0% ITA parameter variations +13% +10% +7% +3% D42 D (active product) (placebo)
4 Extrinsic Skin Aging Skin whitening / Lightening / Evening skin tone Beauty from the inside and out According to Harman s free radical theory of aging, organisms age because cells accumulate free radical damage with the passage of time. UV and free radicals induce inflammation and damage cell content such as DNA, the extracellular matrix as well as the cell membranes resulting in premature aging Melanin synthesis pathways Preventing oxidative damage may delay premature aging Receptor NO, Free radicals UV Tyrosinase Effects of UV Irradiation & Sun Exposure Activated Tyrosinase PGE, IL-1, IL-6 Inflammation Tyrosine DOPAQuinone UV RADIATION SKIN PENETRATION BY TYPE UV-B ( nm) Cause alteration of the physical DNA structure Penetrate until the epidermis Short term redding (sunburns) UV-A ( nm) Lower energy level than UV-B Penetrate until the dermis Triggers cell break down Eumelanins Melanosomes Wound DNA damage Formation of free radicals (ROS) Inflammation Destruction of ECM proteins as collagen Pigmentation
5 IBR-Phyto(flu)ene - PhytoflORAL COLORLESS CAROTENOIDS Natural carotenoids with: Unique anti oxidative capacity UV absorbency = invisible color Anti inflammatory Inhibition of melanin synthesis Inhibition of MMP-1 (collagenase) Prevent damage to DNA Protection and boost of other biomolecules and actives PhytoflORAL Photo Protection & Anti-Aging Protection against UVA & UVB Anti oxidant Anti inflammatory Inhibits collagenase expression and subsequent collagen degradation Prevents damage to DNA Protection & boost of biomolecules Protection & Boost of Sun Screens Phytoene, Phytofluene
6 PhytoflORAL PHOTO PROTECTION & ANTI-AGING - CLINICAL STUDY EVALUATION OF UV PROTECTION POTENTIAL OF A FOOD SUPPLEMENT Aims: To evaluate skin photo-protection due to dietary consumption of PhytoflORAL To evaluate the effect of PhytoflORAL on skin quality To evaluate the subjects appreciation of PhytoflORAL efficacy and characteristics To follow the product s safety Open and intra-individual study n=22 Average age: 24±1 years old (between 20 and 40) Skin photo type II Assessment criteria -> Measurments D0, D42, D84 Dosage 5 mg/day phytoene and phytofluene -Determination of the MED and follow up of its evolution over time. -Before/ After -On the back -Measurements of skin redness with the Spectrocolorimeter -Before/After 1.25 MED irradiation -On the back -Scoring of the skin quality (dryness, roughness, suppleness, evenness of complexion, skin texture). -Subjective evaluation questionnaire. -Before/ After -on the face RESULTS - SUMMARY PhytoflORAL presented a significant photoprotective effect after 84 days of product use, highlighted by a significant increase of the Minimum Erythemal Dose (MED) after UV exposure. After 42 days of PhytoflORAL intake, the Minimum Erythemal Dose (MED) did not change. However, after 84 days of intake, the MED significantly increased, on average (+0.09 mj/cm2, so 10% increase), characterizing a photoprotective effect. This MED increase was observed in 65% of the subjects and increased 20% in average within this group. PhytoflORAL induced an improvement of skin quality after 42 and 84 days of use, shown by Clinical scoring by the dermatologist (significant improvement in skin dryness, roughness, suppleness, eveness and texture) Decrease of skin dryness Decrease of skin roughness Increase of skin suppleness Skin complexion more uniform Improvement of skin texture PhytoflORAL induced an improvement of skin quality after 42 and 84 days of use, shown by: subjective evaluation questionnaire completed by the subjects : subjects found an improvement especially in skin radiance, evenness, hydration, smothness, suppleness, elasticity and irritability. They found their skin more resistant to sun damages, more beautiful and visually healthier. Mean score (out of 10) Evaluation of the skin quality after 42 and 84 days of treatment with product IBRN Skin radiance Skin evenness Skin s resistance to sun damage Skin smoothness Skin hydration Skin suppleness Skin elasticity Skin sensitivity Skin irritability Skin s visible health Skin s overall beauty Skin s visible youthfulness D0 D42 D84
7 INTRODUCTION Natural freeze-dried tomato powder from specially selected, non GMO tomato species rich in the colorless carotenoids phytoene and phytofluene, along with ζ-carotene. PhytoflORAL for Beauty from Within, is functional in protection against UV damage, free radical damage and undesired pigmentation. It thereby contributes to slowing down of aging and photo aging, and to the whitening/ lightening of skin via multiple functions. PhytoflORAL is a complementary oral intake product for IBR's topical use actives (IBR TCLC, IBR CLC and IBR AAC ) and a member of IBR's COLORLESS CAROTENOIDS technology, branded as IBR Phyto(flu)ene. Phytoene and phytofluene, the first carotenoid precursors in the biosynthetic pathway of other carotenoids, has un visible color, absorbing light in the UV range only. The COLORLESS CAROTENOIDS have effective and beneficial activities for beauty applications, specifically in protection against UV and oxidative damage, which is known to lead to pre mature aging and other phenomena. Able to control pigmentation by inhibition of melanin synthesis, COLORLESS CAROTENOIDS brighten and lighten the skin. They also present abilities of inflammation reduction, anti oxidation and UVA/UVB protection that enhance skin lightening. Additionally, COLORLESS CAROTENOIDS are capable of stabilization, protection and boosting of bio molecules. BIOAVAILABILITY AND TISSUE DISTRIBUTION OF PHYTOENE AND PHYTOFLUENE Many dietary carotenoids, both with and without pro vitamin A activity, are found in most human tissues, including in the serum, plasma, and skin. Yet distribution of carotenoids throughout the body is not uniform; when compared to other carotenoids, the COLORLESS CAROTENOIDS phytoene and phytofluene, have the highest accumulation factor in the skin, lungs, plasma, and lymphocytes. This high distribution factor could be an indication for be]er stability of phytoene and phytofluene. It further supports their advantage in ability to prevent and protect against oxidation damage in vivo, and in fact, increased protection of DNA and skin cells against oxidative and UV damage was observed with increased consumption of phytoene and phytofluene.
8 Office & Laboratory: 4 Pekeris St., Tamar Science Park, Rehovot 76702, Israel Tel: Fax: ibroffice@ibrweb.com Business Development & Marketing: Tel: Fax: ibr-marketing@ibrweb.com Organic certified and validated products available IBR. All Rights Reserved This information is based on data we believe to be reliable. It is intended only as a guide for use at the user s discretion and responsibility. It cannot be taken as a license to apply for, or infringe on any patents. FineSchmeckers
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