Beneficial effects of CCR1 blockade on the progression of chronic allograft nephropathy
|
|
- George Daniel
- 7 years ago
- Views:
Transcription
1 Beneficial effects of CCR1 blockade on the progression of chronic allograft nephropathy J. Bedke 1,2, E. Kiss 2, L. Schaefer 3, M. Bonrouhi 2, N. Gretz 4, H.-J. Gröne 2 1 Klinik für Urologie, Universität Heidelberg 2 Abteilung für Zelluläre und Molekulare Pathologie, DKFZ, Heidelberg 3 Institut für Pharmakologie, Universität Frankfurt 4 Zentrum für Medizinische Forschung, Klinikum Mannheim
2 Background Chronic renal allograft (CAN) damage is characterized by persistent activity of interstitial monocytes / macrophages, T-cells and myofibroblasts with subsequent accumulation and deposition of matrix proteins Chemokines and their receptors are linked to the development of CAN The β-chemokine receptor CCR1: expressed on macrophages and T-cells Ligands: CCL3 (MIP-1α), CCL4 (MIP-1β) and RANTES (CCL5) BX 471 is a selective non-peptide antagonist of CCR1, with no crossreactivity to CCR5 Small leucin rich proteoglycan (SLRP): Biglycan: collagen fibril formation and stability pro-inflammatory (TLR2), Il-8 Bedke et al (Am J Transplant): Beneficial effects of CCR1 Blockade on the Progression of Chronic Renal Allograft Damage
3 Background Hypothesis: Chemokines / chemokine receptors exert a major influence on chronic damage in renal allografts by reducing the infiltration of mononuclear cells into the graft and by direct inhibition of matrix synthesis Aim: To analyze the effect of chronic blockade of CCR1 by BX 471 on chronic renal allograft damage
4 Study design Rat allogeneic renal transplantation, F-344 Lewis model, one endogenous kidney Day Tx Group I Group II Treatment: 3 x daily BX mg/kg b.w. or vehicle, s.c.
5 Chronic renal allograft damage Vehicle 42d BX471 42d Tubulointerstitial rejection and transplant glomerulitis of renal allografts at day 42 after transplantation without and with BX 471 treatment (21-42day)
6 Chronic renal allograft damage Glomerulosclerosis Chronic Tubulointerstitial Injury glomerular damage score ** n.s. * tubulointerstitial injury score ** n.s. ** 0 Vehicle BX471 Vehicle BX Vehicle BX471 Vehicle BX d 21-42d 0-21d 21-42d
7 Effect of CCR1 blockade on collagen I Vehicle 42d BX471 42d Collagen I staining of renal allografts at day 42 after transplantation without and with BX 471 treatment (21-42day)
8 Effect of CCR1 blockade on myofibroblast expression Vehicle 42d BX471 42d α-sma SMA staining of renal allografts at day 42 after transplantation without and with BX 471 treatment (21-42day)
9 Effect of BX 471 on myofibroblasts and collagen deposition α-sma Sirius red cells/hpf ** ** ** ** % 50 ** ** n.s. * Vehicle BX471 Vehicle BX471 0 Vehicle BX471 Vehicle BX ** Collagen I n.s. ** 300 Collagen III ** * n.s. score * score * 0 Vehicle BX471 Vehicle BX471 0 Vehicle BX471 Vehicle BX d 21-42d 0-21d 21-42d
10 CCL5 induced biglycan expression in rat macrophages A B (A) Western blot for BGN core protein in macrophage culture supernatants after a 24-h stimulation with CCL5 stimulation ( ng/ml). (B) RT-PCR for BGN expression normalized to GAPDH in macrophages stimulated with CCL5 ( ng/ml) for 4 24 h.
11 Effect of CCR1 blockade on CCL5-induced biglycan expression in rat macrophages RT-PCR for BGN expression normalized to GAPDH in macrophages stimulated for 24 h with CCL5 with and without inhibition of CCR1 by BX 471 (300 nm)
12 Summary The CCR1 antagonist BX 471 can prevent the development of chronic damage in renal allografts This effect is achieved partly by a reduction of infiltration of leukocytes into the graft, partly by an inhibition of the fibrogenic potential of macrophages
13 Conclusion BX 471 CCR1 inhibition my be used for the prevention and treatment of chronic renal allograft damage The β-chemokine receptor CCR1 has a direct proinflammatory and fibrogeneic action in chronic inflammation It remains to be seen, whether this is also true for other chemokines / chemokine receptors Bedke et al. Am J Transplant 2007, 3:
14 Effect of CCR1 blockade on collagen III Vehicle 42d BX471 42d Collagen III staining of renal allografts at day 42 after transplantation without and with BX 471 treatment (21-42day)
15 Renal allograft response cell infiltration CD8+ T-Cells Tubulointerstitium ED1+ Monocytes/Macrophages Tubulointerstitium 150 * *** 100 ** ** cells/hpf cells/hpf Vehicle BX471 Vehicle BX471 Vehicle BX471 0 Vehicle BX471 Vehicle BX471 Vehicle BX d 0-21d 21-42d 0-10d 0-21d 21-42d Effect of BX 471 treatment on ED1+ monocyte/macrophage infiltration
16 Functional parameters Serum Creatinine 3 * 2 mg/dl 1 0 Vehicle BX471 Vehicle BX471 Vehicle BX d 0-21d 21-42d
17 Acute inflammatory allograft response Interstitial Rejection Transplant Glomerulitis tubulointerstitial inflammation score * *** glomerular injury score ** 0 Vehicle BX471 Vehicle BX471 Vehicle BX471 0 Vehicle BX471 Vehicle BX471 Vehicle BX d 0-21d 21-42d 0-10d 0-21d 21-42d
18 Effect of BX 471 on biglycan expression in rat macrophages in vivo of transplanted kidneys Vehicle BX 471 Biglycan mrna expression by in situ hybridization in untreated and BX471 treated allografts at day 21 after transplantation (200x)
19 Molecular structure of BX 471 BX 471 a CCR1 antagonist: : R-N-[5R [5-chloro-2-[2-[4-[(4-fluorophenyl)methyl] fluorophenyl)methyl]- 2-methyl-1-piperazinyl] piperazinyl]-2-oxoethoxy]phenyl]urea hydrochloric acid salt (Liang M et al. JBC 2000)
20 Chronic renal allograft damage Vehicle 21d BX471 21d Preglomerular artery of renal allografts at day 21 after transplantation without and with BX 471 treatment (0-21day)
21 Allograft response of fibrosis-associated genes PAI-1 TGF-β1 Mean Fluorescence Mean Fluorescence 0.02 ** ** Vehicle BX471 Vehicle BX Vehicle BX471 Vehicle BX d 21-42d Real-time RT-PCR 0-21d 21-42d
Drug Development Services
Drug Development Services USING BLOOD AND BONE MARROW PRIMARY CELL SYSTEMS Clinically Relevant In Vitro Assays Broad Spectrum of Drug Classes Multi-Species Platforms Enhancing Drug Development through
More informationEFFECTS OF IRON ON THE IMMUNE SYSTEM
EFFECTS OF IRON ON THE IMMUNE SYSTEM Gert Mayer Department of Internal Medicine IV (Nephrology and Hypertension) Medical University Innsbruck Austria Conflicts of interest Research Support: Amgen, Roche,
More informationTechnological platforms
Advitech Advitech is a life sciences & technology company Its mission is to discover and commercialize proprietary and evidence-based natural health products Focus on milk, whey and bovine colostrum R&D,
More informationOKT3. ~ The first mouse monoclonal antibody. used in clinical practice in the field of transplantation ~
g944202 潘 怡 心 OKT3 ~ The first mouse monoclonal antibody used in clinical practice in the field of transplantation ~ As everybody knows, OKT3 is the first mouse monoclonal antibody produced for the treatment
More information11/7/2011. Nothing to disclose. Cellular origin of fibrosis. 1. Fibroblasts. 2. Epithelial Mesenchymal Transformation
/7/ T cell dependent regulation of fibrogenesis Financial disclosure Matthias Mack University Hospital Regensburg, Germany Nothing to disclose Cellular origin of fibrosis Acitvation of fibroblasts EMT
More informationSDS gel electrophoresis was performed using a 4% by 20% gradient gel 8. Quantification of Western blots was performed using Image J Processing and
Supplemental Material: Western blot: SDS gel electrophoresis was performed using a 4% by 20% gradient gel 8. Quantification of Western blots was performed using Image J Processing and Analysis (NIH). Quantitative
More informationMechanism for Dehydration Associated Kidney Disease
Mechanism for Dehydration Associated Kidney Disease Richard J Johnson, University of Colorado A sugarcane worker dying from kidney failure Dr Johnson has received funding from Danone Research Chronic Kidney
More informationRheumatoid arthritis: an overview. Christine Pham MD
Rheumatoid arthritis: an overview Christine Pham MD RA prevalence Chronic inflammatory disease affecting approximately 0.5 1% of the general population Prevalence is higher in North America (approaching
More informationVitamin D deficiency exacerbates ischemic cell loss and sensory motor dysfunction in an experimental stroke model
Vitamin D deficiency exacerbates ischemic cell loss and sensory motor dysfunction in an experimental stroke model Robyn Balden & Farida Sohrabji Texas A&M Health Science Center- College of Medicine ISC
More informationIn vitro co-culture model of the inflamed intestinal mucosa
In vitro co-culture model of the inflamed intestinal mucosa Berlin, December 13, 2011 Eva-Maria Collnot, e.collnot@mx.uni-saarland.de Helmholtz Institute for Pharmaceutical Research Saarland Departement
More informationAriela Benigni. Biol.Sci.D., Ph.D. Curriculum Vitae
Ariela Benigni Biol.Sci.D., Ph.D. Curriculum Vitae Personal Data Name: Date and place of birth: Citizenship: E-mail: Ariela Benigni December 16, 1955 - Bergamo, Italy Italian ariela.benigni@marionegri.it
More informationEffets biologiques des facteurs de croissance sur la régénération musculaire
Effets biologiques des facteurs de croissance sur la régénération musculaire Xavier Bigard (MD, PhD) Professeur Agrégé du Val-de-Grâce Institut de Recherche Biomédicale des Armées (IRBA) La Tronche (Grenoble)
More informationA Genetic Analysis of Rheumatoid Arthritis
A Genetic Analysis of Rheumatoid Arthritis Introduction to Rheumatoid Arthritis: Classification and Diagnosis Rheumatoid arthritis is a chronic inflammatory disorder that affects mainly synovial joints.
More informationInflammation and wound healing in presence of biomaterials
Inflammation and wound healing in presence of biomaterials Gemma Mestres Applied Materials in Medicine, Department of Engineering Science gemma.mestres@angstrom.uu.se Outline Why is it important? 1. Immunity
More informationPlatelet Review July 2012. Thomas S. Kickler M.D. Johns Hopkins University School of Medicine
Platelet Review July 2012 Thomas S. Kickler M.D. Johns Hopkins University School of Medicine Hemostasis Hemostasis is the process that leads to the stopping of bleeding Hemostasis involves blood vessels,
More informationTreatment of antibody-mediated rejection with eculizumab
Treatment of antibody-mediated rejection with eculizumab Josep M Cruzado, MD Nephrology Department Hospital Universitari de Bellvitge jmcruzado@bellvitgehospital.cat @crz_garrit Diagnostic criteria for
More informationRenal syndromes leading to abnormal kidney function:
Renal syndromes leading to abnormal kidney function: An abnormality in kidney function can be detected by the following: 1) changes in serum creatinine concentration, reflecting changes in GFR 2) abnormalities
More informationImmunosuppressive drugs
Immunosuppressive drugs RJM ten Berge Afd. Inw. Geneeskunde AMC contents overview targets mechanism of action Effects on immune capacity measured by responses to vaccination Immune response 1 calcineurin
More informationInflammation and Healing. Review of Normal Defenses. Review of Normal Capillary Exchange. BIO 375 Pathophysiology
Inflammation and Healing BIO 375 Pathophysiology Review of Normal Defenses Review of Normal Capillary Exchange 1 Inflammation Inflammation is a biochemical and cellular process that occurs in vascularized
More informationDietary emulsifiers impact the mouse gut microbiota promoting colitis and metabolic syndrome
Dietary emulsifiers impact the mouse gut microbiota promoting colitis and metabolic syndrome Andrew T. Gewirtz et al N A T U R E VOL 519 5 M A R C H 2 0 1 5 Background Incidence of IBD Fact 1: increasing
More informationThe sensitive marker for glomerular filtration rate (GFR) Estimation of GFR from Serum Cystatin C:
The sensitive marker for glomerular filtration rate (GFR) Estimation of GFR from Serum Cystatin C: The good correlation allows close estimation of GFR Cystatin C GFR GFR in serum estimated* measured* n
More informationTHE ENDOCANNABINOID SYSTEM AS A THERAPEUTIC TARGET FOR LIVER DISEASES. Key Points
December 2008 (Vol. 1, Issue 3, pages 36-40) THE ENDOCANNABINOID SYSTEM AS A THERAPEUTIC TARGET FOR LIVER DISEASES By Sophie Lotersztajn, PhD, Ariane Mallat, MD, PhD Inserm U841, Hôpital Henri Mondor,
More informationRelative Quantification of mirna Target mrnas by Real-Time qpcr. 1 Introduction. Gene Expression Application Note No. 4
Gene Expression Application Note No. 4 Relative Quantification of mirna Target mrnas by Real-Time qpcr Ute Ernst, Jitao David Zhang, Anja Irsigler, Stefan Wiemann, Ulrich Tschulena Division: Molecular
More informationBiological Imaging : Gene-Diagnosis-Therapy. M. Schwaiger * Technische Universität Munich Germany. European Commission 7th Framework
Biological Imaging : Gene-Diagnosis-Therapy M. Schwaiger * Technische Universität Munich Germany European Commission 7th Framework Pa Biological Imaging Hypotheses Research tool in basic and clinical sciences
More informationExpression of CD163 on Bovine Alveolar Macrophages and Peripheral Blood Mononuclear Cells
Expression of CD163 on Bovine Alveolar Macrophages and Peripheral Blood Mononuclear Cells Mary E. Kopechek Honor s Research Thesis May 17, 2007 Research Advisor: Dr. Jeff Lakritz, DVM, PhD The Ohio State
More informationRayBio Human IL-8 ELISA Kit
RayBio Human IL-8 ELISA Kit User Manual (for Cell Lysate and Tissue Lysate) (Revised Mar 1, 2012) RayBio Human IL-8 ELISA Kit Protocol (Cat#: ELH-IL8-001C) RayBiotech, Inc. We Provide You With Excellent
More informationBIOM 255 - Firestein, Winter 2009. Rheumatoid arthritis. Page 1
Rheumatoid arthritis Page 1 Vascular events in RA Synovium in rheumatoid arthritis Non-extra articular RA Extra articular RA Turesson, et al. Ann Rheum Dis. 2007;66:60 Normal Rheumatoid Arthritis Major
More informationResearch Group. Towards small-molecule ligands to modulate leukocyte behavior
Research Group Receptor-Ligand-Interactions Towards small-molecule ligands to modulate leukocyte behavior Dr. Katja Schmitz, 28.07.09 Chemokines and chemokine receptors Chemotactic cytokines Small, soluble
More informationImmunology and immunotherapy in allergic disease
Immunology and immunotherapy in allergic disease Jing Shen, MD Faculty Advisor: Matthew Ryan, MD The University of Texas Medical Branch Department of Otolaryngology Grand Rounds Presentation February 2005
More informationRayBio Human TNF-alpha
RayBio Human TNF-alpha ELISA Kit User Manual (Revised Mar 1, 2012) RayBio Human TNF-alpha ELISA Kit Protocol (Cat#: ELH-TNFalpha-001) RayBiotech, Inc. We Provide You With Excellent Protein Array System
More informationIKDT Laboratory. IKDT as Service Lab (CRO) for Molecular Diagnostics
Page 1 IKDT Laboratory IKDT as Service Lab (CRO) for Molecular Diagnostics IKDT lab offer is complete diagnostic service to all external customers. We could perform as well single procedures or complex
More informationAutoimmunity and immunemediated. FOCiS. Lecture outline
1 Autoimmunity and immunemediated inflammatory diseases Abul K. Abbas, MD UCSF FOCiS 2 Lecture outline Pathogenesis of autoimmunity: why selftolerance fails Genetics of autoimmune diseases Therapeutic
More informationA disease and antibody biology approach to antibody drug discovery
A disease and antibody biology approach to antibody drug discovery Björn Frendéus, PhD VP, Preclinical research Presenter: Björn Frendéus Date: 2011-11-08 1 Antibodies have revolutionized Cancer Treatment!
More informationFGF-1 as Cosmetic Supplement
FGF-1 as Cosmetic Supplement Ing-Ming Chiu, Ph.D. Professor, Internal Medicine and Molecular and Cellular Biochemistry The Ohio State University Columbus, Ohio, U.S.A. GENTEON USA Fibroblast Growth Factor
More informationHYPERTENSION ASSOCIATED WITH RENAL DISEASES
RENAL DISEASE v Patients with renal insufficiency should be encouraged to reduce dietary salt and protein intake. v Target blood pressure is less than 135-130/85 mmhg. If patients have urinary protein
More informationMicroRNA formation. 4th International Symposium on Non-Surgical Contraceptive Methods of Pet Population Control
MicroRNA formation mirna s are processed from several precursor stages Mammalian genomes seem to have 100 s of mirna s Nucleotides in positions 2-8 of an mirna are considered the mirna seed 5 Methyl-G
More informationCD3/TCR stimulation and surface detection Determination of specificity of intracellular detection of IL-7Rα by flow cytometry
CD3/TCR stimulation and surface detection Stimulation of HPB-ALL cells with the anti-cd3 monoclonal antibody OKT3 was performed as described 3. In brief, antibody-coated plates were prepared by incubating
More informationder Ludwig-Maximilians-Universität München ehem. Direktor: Prof. Dr. med. Detlef Schlöndorff jetziger komm. Direktor: Prof. Dr. med.
Aus der Medizinischen Poliklinik Innenstadt der Ludwig-Maximilians-Universität München ehem. Direktor: Prof. Dr. med. Detlef Schlöndorff jetziger komm. Direktor: Prof. Dr. med. Martin Reincke The role
More informationHead of College Scholars List Scheme. Summer Studentship. Report Form
Head of College Scholars List Scheme Summer Studentship Report Form This report should be completed by the student with his/her project supervisor. It should summarise the work undertaken during the project
More informationThe antiinflammatory action mechanism of an antihomotoxic composita remedy
The antiinflammatory action mechanism of an antihomotoxic composita remedy H. Heine, F. Andrä [Ärztezeitschrift für Naturheilverfahren 43, 2 (22)] [Summary] Introduction In order to use preparations in
More informationPrognostic impact of uric acid in patients with stable coronary artery disease
Prognostic impact of uric acid in patients with stable coronary artery disease Gjin Ndrepepa, Siegmund Braun, Martin Hadamitzky, Massimiliano Fusaro, Hans-Ullrich Haase, Kathrin A. Birkmeier, Albert Schomig,
More informationCELLULE STELLATE EPATICHE E DANNO METABOLICO. Fabio Marra Dipartimento di Medicina Interna Centro di Eccellenza DenoTHE Università di Firenze
CORSO UNIGASTRO San Vincenzo 4-6 Maggio 2008 CELLULE STELLATE EPATICHE E DANNO METABOLICO Fabio Marra Dipartimento di Medicina Interna Centro di Eccellenza DenoTHE Università di Firenze ITALY Bataller
More informationKMS-Specialist & Customized Biosimilar Service
KMS-Specialist & Customized Biosimilar Service 1. Polyclonal Antibody Development Service KMS offering a variety of Polyclonal Antibody Services to fit your research and production needs. we develop polyclonal
More informationOvervekt, mer enn bare overvekt
Overvekt, mer enn bare overvekt Kurs Overvekt i svangerskap og fødsel Overvekt/fedme i historisk perspektiv I løpet av1-2 generasjoner har det vært en økning i forekomsten av fedme som er historisk uovertroffen.
More informationGranulocytes vs. Agranulocytes
Leukocytes are white blood cells (AKA colorless (non-pigmented) blood cells). (Much) smaller in number than RBCs. Unlike RBCs, there are several different types of WBCs. All contain a visible nucleus.
More informationLes liasons dangerouses: infiammazione e cancro
Dipartimento di Diagnostica per Immagini e Medicina di Laboratorio Department of Morphology, Surgery and Experimental Medicine Center of Excellence MIUR for the Study of Inflammation (ICSI) Ferrara University
More informationFIBROSE RENALE ET PERSPECTIVES THERAPEUTIQUES
FIBROSE RENALE ET PERSPECTIVES THERAPEUTIQUES Tissue fibrosis uncontrolled repair of damaged tissues Infections Lesions Burns Mechanic stress Radiation Hypertension Alcohol Metabolic disease Auto-immune
More informationHow do plasma cells survive
How do plasma cells survive Claudia Berek Deutsches Rheuma Forschungs Zentrum, Berlin Institut der Leibniz Gemeinschaft Immune response Naive B cell Peripheral lymphoid organs Memory B cell Germinal T
More informationIdiopathic Pulmonary Fibrosis (IPF) Research
Idiopathic Pulmonary Fibrosis (IPF): Why Early Referral is Critical Even if Your Patient is Not Eligible for a Clinical Trial Idiopathic Pulmonary Fibrosis (IPF) Research Management of IPF requires a confident
More informationPPAR-γ agonist pioglitazone affects rat gouty arthritis by regulating cytokines
PPAR-γ agonist pioglitazone affects rat gouty arthritis by regulating cytokines R.-C. Wang and D.-M. Jiang Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing,
More informationAngelika Falsone Mobile: ++43 (0)660 5589966
Mobile: ++43 (0)660 5589966 Schöcklblickstrasse 8a, A-8062 Kumberg, Austria angelikafalsone@gmail.com Personal Statements Date of birth 29.04.1976 Place of birth Leoben Citizenship Austria Marital status
More informationDepartment of Nephrology and Immunology (Medizinische Klinik II) RWTH University Clinic Aachen, Germany
Department of Nephrology and Immunology (Medizinische Klinik II) RWTH University Clinic Aachen, Germany Aachen, Germany RWTH Aachen University Hospital NAME OF THE UNIT: Department of Nephrology and Immunology
More informationVPM 152. INFLAMMATION: Chemical Mediators
General Pathology VPM 152 INFLAMMATION: Chemical Mediators CHEMICAL MEDIATORS OF INFLAMMATION Definition: any messenger that acts on blood vessels, inflammatory cells or other cells to contribute to an
More informationOsteoblast Differentiation and Mineralization
Osteoblast Differentiation and Mineralization Application Note Background Osteoblasts are specialized fibroblasts that secrete and mineralize the bone matrix. They develop from mesenchymal precursors.
More informationPLACENTA UMANA da materiale di scarto biologico.. verso il loro utilizzo per la Terapia Rigenerativa
PLACENTA UMANA da materiale di scarto biologico.. verso il loro utilizzo per la Terapia Rigenerativa Ornella Parolini Centro di Ricerca E.Menni Fondazione Poliambulanza Brescia Amnion Smooth chorion False
More informationStem Cell Transplantation
Harmony Behavioral Health, Inc. Harmony Behavioral Health of Florida, Inc. Harmony Health Plan of Illinois, Inc. HealthEase of Florida, Inc. Ohana Health Plan, a plan offered by WellCare Health Insurance
More informationFattori implicati nel rimodellamento tissutale epatico
tissutale epatico Dr. Simone Carotti Unità di Ricerca di Anatomia Microscopica e Ultrastrutturale Facoltà Dipartimentale di Medicina e Chirurgia Background Duncan AW, Dorrell C, Grompe M. Gastroenterology.
More informationCytotoxic T lymphocytes and natural killer cells
Cytotoxic T lymphocytes and natural killer cells Margrit Wiesendanger Division of General Medicine, CUMC September 16, 2009 Killer cells: CD8 + T cells (adaptive) vs. natural killer (innate) Shared purpose:
More informationHow To Get A Cell Print
QUICK CELL CAPTURE AND CHARACTERIZATION GUIDE FOR CELLSEARCH CUSTOMERS CellSave EDTA Blood sample Rare cell capture Enumeration Single protein marker Cell capture for molecular characterization CELLSEARCH
More informationSupplemental Information. McBrayer et al. Supplemental Data
1 Supplemental Information McBrayer et al. Supplemental Data 2 Figure S1. Glucose consumption rates of MM cell lines exceed that of normal PBMC. (A) Normal PBMC isolated from three healthy donors were
More informationGene Silencing Oligos (GSOs) Third Generation Antisense
Gene Silencing Oligos (GSOs) Third Generation Antisense Walter R. Strapps, Ph.D. Executive Director, RNA Therapeutics Idera Pharmaceuticals Cambridge, MA NASDAQ: IDRA www.iderapharma.com Idera is a leader
More information27E10: A unique monoclonal antibody against MRP8/14 (S100A8/A9, Calprotectin)
BMA BIOMEDICALS Rheinstrasse 28-32 CH-4302 Augst (Switzerland) Phone:+41 61 811 6222 Fax: +41 61 811 6006 info@bma.ch www.bma.ch 27E10: A unique monoclonal antibody against MRP8/14 (S100A8/A9, Calprotectin)
More informationHow to construct transgenic mice
How to construct transgenic mice Sandra Beer-Hammer Autumn School 2012 Bad Schandau Pharmakologie und Experimentelle Therapie (APET) Overview History Generation of embryonic stem (ES) cell lines Generation
More informationDendritic Cells: A Basic Review *last updated May 2003
*last updated May 2003 Prepared by: Eric Wieder, PhD MD Anderson Cancer Center Houston, TX USA What is a dendritic cell? Dendritic cells are antigen-presenting cells (APCs) which play a critical role in
More informationChronic Kidney Disease and Diabetes
Anyone with diabetes can get kidney disease. Diabetes and high blood pressure are the most common causes of kidney disease, and people often have both. Chronic (long term) kidney disease (CKD) caused by
More informationNatalia Taborda Vanegas. Doc. Sci. Student Immunovirology Group Universidad de Antioquia
Pathogenesis of Dengue Natalia Taborda Vanegas Doc. Sci. Student Immunovirology Group Universidad de Antioquia Infection process Epidermis keratinocytes Dermis Archives of Medical Research 36 (2005) 425
More informationIn Vivo and In Vitro Screening for Thyroid Hormone Disruptors
In Vivo and In Vitro Screening for Thyroid Hormone Disruptors Kevin M. Crofton National lhealth and Environmental Research Laboratory California Environmental Protection Agency Human Health Hazard Indicators
More informationIntroduction. Pathogenesis of type 2 diabetes
Introduction Type 2 diabetes mellitus (t2dm) is the most prevalent form of diabetes worldwide. It is characterised by high fasting and high postprandial blood glucose concentrations (hyperglycemia). Chronic
More informationSuppl. Figure 1. Cells cultured in oxldl take up Oil Red O in a time-dependent
Suppl. Figure 1. Cells cultured in oxldl take up Oil Red O in a time-dependent manner. Uptake of Oil Red O was performed as detailed in Materials and Methods and absorbance of samples was expressed as
More informationSouthern Derbyshire Shared Care Pathology Guidelines. Hyperkalaemia
Southern Derbyshire Shared Care Pathology Guidelines Hyperkalaemia Purpose of Guideline Dealing with adult patients with Hyperkalaemia in the community Definition Serum potassium normal range is 3.5 5.3
More informationChanges in liver volume as determined by MRI scans after two weeks of a low energy diet (t0: before start of the diet; t1: after two weeks of diet)
Trial Description Title Influence of different preoperative low energy diets on changes in liver volume, liver fat content, visceral fat and body composition as well as liver and adipose tissue mrna expression
More informationCytokines: Physiology and Clinical Relevance Pranay D. Khare, Ph.D. and Meenakshi Khare, Ph.D.
Cytokines: Physiology and Clinical Relevance Pranay D. Khare, Ph.D. and Meenakshi Khare, Ph.D. A shift in medicine is taking place. It is characterized by the emergence of neuroendoimmunology (NEI), a
More informationPulling the Plug on Cancer Cell Communication. Stephen M. Ansell, MD, PhD Mayo Clinic
Pulling the Plug on Cancer Cell Communication Stephen M. Ansell, MD, PhD Mayo Clinic Why do Waldenstrom s cells need to communicate? Waldenstrom s cells need activating signals to stay alive. WM cells
More informationDEPARTMENT OF IMMUNOLOGY Chair of Histology and Immunology Medical University of Gdańsk
DEPARTMENT OF IMMUNOLOGY Chair of Histology and Immunology Medical University of Gdańsk Research group Head: Jolanta Myśliwska, MD, PhD Professor of immunology Joanna Więckiewicz, M. Sc. Dominik Rachoń,
More informationUnderstanding the immune response to bacterial infections
Understanding the immune response to bacterial infections A Ph.D. (SCIENCE) DISSERTATION SUBMITTED TO JADAVPUR UNIVERSITY SUSHIL KUMAR PATHAK DEPARTMENT OF CHEMISTRY BOSE INSTITUTE 2008 CONTENTS Page SUMMARY
More informationEssentials of Real Time PCR. About Sequence Detection Chemistries
Essentials of Real Time PCR About Real-Time PCR Assays Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data is therefore collected
More information岑 祥 股 份 有 限 公 司 技 術 專 員 費 軫 尹 20100803
技 術 專 員 費 軫 尹 20100803 Overview of presentation Basic Biology of RNA interference Application of sirna for gene function? How to study mirna? How to deliver sirna and mirna? New prospects on RNAi research
More informationAnti-Inflammatory Drugs from Plant and Marine Sources
D. A. Lewis Anti-Inflammatory Drugs from Plant and Marine Sources 1989 BirkhauserVerlag Basel Boston Berlin TABLE OF CONTENTS Page No. Preface. Acknowledgements..12 SECTION I : INFLAMMATION AND INFLAMMATORY
More informationEnvironmental, genetic and immunological determinants of Rheumatoid Arthritis
University of Zurich University Hospital of Zurich Environmental, genetic and immunological determinants of Rheumatoid Arthritis FORALLVENT Symposium Basel, 2007 Diego Kyburz, M.D. Division of Rheumatology
More informationR EFERENCES. Summary and conclusions
R EFERENCES Summary and conclusions 129 S UMMARY AND CONCLUSIONS SUMMARY AND GENERAL DISCUSSION 130 Constant vasodilatation, inhibition of platelet and monocyte adhesion, and local thrombolysis are the
More informationHow to construct transgenic mice
How to construct transgenic mice Sandra Beer-Hammer Autumn School 2010 Bad Schandau Methods additional genetic information transgenic mouse line gene inactivation gene-deficient knockout mouse line Jak2
More informationD. Vitamin D. 1. Two main forms; vitamin D2 and D3
D. Vitamin D. Two main forms; vitamin D2 and D3 H H D3 - Cholecalciferol D2 - Ergocalciferol Technically, vitamin D is not a vitamin. It is the name given to a group of fat-soluble prohormones (substances
More informationIP10 Human ELISA Kit
ab100579 IP10 Human ELISA Kit Instructions for Use For the quantitative measurement of Human IP10 concentrations in serum, plasma and cell culture supernatants This product is for research use only and
More informationBasics of Immunology
Basics of Immunology 2 Basics of Immunology What is the immune system? Biological mechanism for identifying and destroying pathogens within a larger organism. Pathogens: agents that cause disease Bacteria,
More informationGene Expression Assays
APPLICATION NOTE TaqMan Gene Expression Assays A mpl i fic ationef ficienc yof TaqMan Gene Expression Assays Assays tested extensively for qpcr efficiency Key factors that affect efficiency Efficiency
More information2.1.2 Characterization of antiviral effect of cytokine expression on HBV replication in transduced mouse hepatocytes line
i 1 INTRODUCTION 1.1 Human Hepatitis B virus (HBV) 1 1.1.1 Pathogenesis of Hepatitis B 1 1.1.2 Genome organization of HBV 3 1.1.3 Structure of HBV virion 5 1.1.4 HBV life cycle 5 1.1.5 Experimental models
More informationBasic Science in Medicine
Medical Journal of th e Islamic Republic of Iran Volume 18 Number 3 Fall 1383 November 2004 Basic Science in Medicine ] EXPANSION OF HUMAN CORD BLOOD PRIMITIVE PROGENITORS IN SERUM-FREE MEDIA USING HUMAN
More informationHiPerFect Transfection Reagent Handbook
Fifth Edition October 2010 HiPerFect Transfection Reagent Handbook For transfection of eukaryotic cells with sirna and mirna Sample & Assay Technologies QIAGEN Sample and Assay Technologies QIAGEN is the
More informationPREPARAZIONE DI UN KIT DIAGNOSTICO COMMERCIALE PER IL DOSAGGIO SIERICO DEL mir-148b NELLA MALATTIA RENALE: IMMUNOGLOBULINA A NEFROPATIA (IgAN)
PREPARAZIONE DI UN KIT DIAGNOSTICO COMMERCIALE PER IL DOSAGGIO SIERICO DEL mir-148b NELLA MALATTIA RENALE: IMMUNOGLOBULINA A NEFROPATIA (IgAN) F.P. Schena Università degli Studi di Bari; Consorzio C.A.R.S.O.
More informationrenal transplantation: A single-center comparative study
Impact of posterior urethral valves on pediatric renal transplantation: A single-center comparative study BY Mohamed Kamal Gheith, MD Oberarzt die Urologie, Universitätsmedizin Mainz Ass. Prof. of Urology,
More informationSHAHID AZIZ DO, FACOI.
SHAHID AZIZ DO, FACOI. ASSOCIATE CLINICAL PROFESSOR OF GI AND LIVER DISEASE. UT SOUTHWESTERN HEALTH SCIENCE CENTER, DALLAS ADJUNCT CLINICAL ASSISTANT PROFESSOR DEPT OF MEDICINE UNT HEALTH SCIENCE CENTER
More informationANTIBODY AND CYTOKINE DEVELOPMENTS AND SERVICES. Zsolt Keresztessy, PhD MBA TUDOMÁNYOS HÉTFŐ 2012.04.23.
ANTIBODY AND CYTOKINE DEVELOPMENTS AND SERVICES AT DEBRECEN CLINICAL GENOMICS CENTER DEPT BIOCHEMISTRY AND MOLECULAR BIOLOGY Zsolt Keresztessy, PhD MBA TUDOMÁNYOS HÉTFŐ 2012.04.23. MISSION CUTTING EDGE
More informationThe Unmet Medical Kidney Transplant Recipients: The Causes of Long Term Graft Failure
The Unmet Medical Kidney Transplant Recipients: The Causes of Long Term Graft Failure Roslyn B. Mannon, M.D. University of Alabama at Birmingham FDA Meeting on Surrogate Endpoints Relevant Financial Relationship
More informationFinal Review. Aptamers. Making Aptamers: SELEX 6/3/2011. sirna and mirna. Central Dogma. RNAi: A translation regulation mechanism.
Central Dogma Final Review Section Week 10 DNA RNA Protein DNA DNA replication DNA RNA transcription RNA Protein translation **RNA DNA reverse transcription http://bass.bio.uci.edu/~hudel/bs99a/lecture20/lecture1_1.html
More informationAcute on Chronic Liver Failure: Current Concepts. Disclosures
Acute on Chronic Liver Failure: Current Concepts Vandana Khungar, MD MSc Assistant Professor of Medicine University of Pennsylvania, Perelman School of Medicine September 20, 2015 None to declare Disclosures
More informationNew Approaches to a Major Public-Health Problem Infectious Diseases. Ethan Weiner, M.D. Senior Vice President Therapeutic Area Development Head
New Approaches to a Major Public-Health Problem Infectious Diseases Ethan Weiner, M.D. Senior Vice President Therapeutic Area Development Head Overview The Burden Of Infectious Disease Is Large And May
More informationScientific contact information: Ignacio Anegon (ianegon@nantes.inserm.fr) or Arnaud Nicot (arnaud.nicot@inserm.fr)
Transplantation and Immunology Nantes, France "Progreffe 2012" Inserm UMRS 1064/ITUN [www.itun.nantes.inserm.fr/index.php/en] in Nantes (France) is launching its International doctoral program by opening
More informationAAGPs TM Anti-Aging Glyco Peptides. Enhancing Cell, Tissue and Organ Integrity Molecular and biological attributes of lead AAGP molecule
AAGPs TM Anti-Aging Glyco Peptides Enhancing Cell, Tissue and Organ Integrity Molecular and biological attributes of lead AAGP molecule 1 Acknowledgements This presentation was prepared by Dr. Samer Hussein
More informationT Cell Maturation,Activation and Differentiation
T Cell Maturation,Activation and Differentiation Positive Selection- In thymus, permits survival of only those T cells whose TCRs recognize self- MHC molecules (self-mhc restriction) Negative Selection-
More informationTwincore - Zentrum für Experimentelle und Klinische Infektionsforschung Institut für Molekulare Bakteriologie
Twincore - Zentrum für Experimentelle und Klinische Infektionsforschung Institut für Molekulare Bakteriologie 0 HELMHOLTZ I ZENTRUM FÜR INFEKTIONSFORSCHUNG Technische Universität Braunschweig Institut
More information