Axl Antibody AbVantage Pack

Transcription

1 Axl Antibody AbVantage Pack Produced in Rabbit Catalog No. A30-774A NP_ CONTENTS Rabbit anti-axl A302-67A Rabbit anti-axl A302-68A WB, IP REACTIVITY STORAGE / SHELF LIFE 2-8 C / year from date of receipt NOTE (FAX) info@bethyl.com

2 Axl Antibody Rabbit Polyclonal Catalog No. A302-67A NP_ REACTIVITY TESTED PRESUMED REACTIVITY ISOTYPE AMOUNT STORAGE/SHELF LIFE BUFFER PRODUCTION PROCEDURES WB Based on 00% sequence identity, this antibody is predicted to react with Gorilla. IgG 00 µl at mg/ml 2-8 C / year from date of receipt Tris-citrate/phosphate buffer, ph 7 to 8 containing 0.09% Sodium Azide Antibody was affinity purified using an epitope specific to Axl immobilized on solid support. The epitope recognized by A302-67A maps to a region between residue 250 and 300 of human AXL receptor tyrosine kinase using the numbering given in entry NP_ (GeneID ). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of.4 equals.0 mg of IgG. APPLICATION NOTES ADDITIONAL INFO Western Blot :2,000 - :0,000 Immunoprecipitation Not recommended. Use A302-68A. Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel and ReliaBLOT Reagents (Cat. No. WB20). Use the link above to view SDS, a current list of citations, and other product specific information.

3 Axl Antibody: A302-67A Detection of Axl by Western Blot. Samples: Whole cell lysate (5, 5 and 50 µg) from HeLa cells. Antibodies: Affinity purified rabbit anti-axl antibody A302-67A used for WB at 0. µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. 2

4 Axl Antibody Rabbit Polyclonal Catalog No. A302-68A NP_ REACTIVITY TESTED PRESUMED REACTIVITY ISOTYPE AMOUNT STORAGE/SHELF LIFE BUFFER PRODUCTION PROCEDURES WB, IP Based on 00% sequence identity, this antibody is predicted to react with Rhesus Monkey, Green monkey and Crab-eating macaque. IgG 00 µl at 0.2 mg/ml 2-8 C / year from date of receipt Tris-buffered Saline with 0.% BSA containing 0.09% Sodium Azide Antibody was affinity purified using an epitope specific to Axl immobilized on solid support. The epitope recognized by A302-68A maps to a region between residue 844 and 894 of human AXL receptor tyrosine kinase using the numbering given in entry NP_ (GeneID ). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of.4 equals.0 mg of IgG. APPLICATION NOTES ADDITIONAL INFO Western Blot :2,000 - :0,000 Immunoprecipitation 5 to 5 µg/mg lysate Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel and ReliaBLOT Reagents (Cat. No. WB20). Use the link above to view SDS, a current list of citations, and other product specific information.

5 Axl Antibody: A302-68A Detection of Axl by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 5 and 50 µg for WB; mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-axl antibody A302-68A used for WB at 0.04 µg/ml (A) and µg/ml (B) and used for IP at 0 µg/mg lysate. Axl was also immunoprecipitated by rabbit anti-axl antibody A302-67A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A and B). 2