CHROMATOGRAPHY III Pharm.D Department Of Pharmaceutical Analysis SRM College of Pharmacy,Kattankulathur

Transcription

1 CHROMATOGRAPHY III Pharm.D Department Of Pharmaceutical Analysis SRM College of Pharmacy,Kattankulathur

2 THIN LAYER CHROMATOGRAPHY (TLC)

3 THIN LAYER CHROMATOGRAPHY Thin layer chromatography (TLC) is an important technique for identification and separation of mixtures of organic compounds. It is useful in: Identification of components of a mixture (using appropriate standards) following the course of a reaction, analyzing fractions collected during purification, analyzing the purity of a compound. In TLC, components of the mixture are partitioned between an adsorbent (the stationary phase, usually silica gel, SiO 2 ) and a solvent ( the mobile phase) which flows through the adsorbent.

4 THIN LAYER CHROMATOGRAPHY In TLC, a plastic, glass or aluminum sheet is coated with a thin layer of silica gel. A very small amount of a solution of the substance to be analyzed is applied in a small spot with a capillary tube, ~1cm from the bottom of the TLC plate The TLC is developed in a chamber which contains the developing solvent (the mobile phase). A truncated filter paper placed in the chamber serves to saturate the chamber with mobile phase. A B U C D filter paper A B U C D

5 THIN LAYER CHROMATOGRAPHY As the mobile phase rises up the TLC plate by capillary action, the components dissolve in the solvent and move up the TLC plate. Individual components move up at different rates, depending on intermolecular forces between the component and the silica gel stationary phase and the component and the mobile phase. The stationary phase is SiO2 and is very polar. It is capable of strong dipole-dipole and H-bond donating and accepting interactions with the analytes (the components being analyzed). More polar analytes interact more strongly with the stationary phase in move very slowly up the TLC plate. By comparison, the mobile phase is relatively nonpolar and is capable of interacting with analytes by stronger London forces, as well as by dipoledipole and H-bonding. More nonpolar analytes interact less strongly with the polar silica gel and more strongly with the less polar mobile phase and move higher up the TLC plate.

6 THIN LAYER CHROMATOGRAPHY Once the solvent is within ~1-2 cm of the top of the TLC sheet, the TLC is removed from the developing chamber and the farthest extent of the solvent (the solvent front) is marked with a pencil. The solvent is allowed to evaporate from the TLC sheet in the hood. The spots are visualized using a UV lamp. A fluorescent compound, usually Manganeseactivated Zinc Silicate, is added to the adsorbent that allows the visualization of spots under a blacklight (UV254). The adsorbent layer will fluoresce light green by itself, but spots of analyte quench this fluorescence and appear as a dark spot.

7 THIN LAYER CHROMATOGRAPHY - Visualization As the chemicals being separated may be colorless, several methods exist to visualize the spots: Visualization of spots under a UV 254 lamp. The adsorbent layer will thus fluoresce light green by itself, but spots of analyte quench this fluorescence. Iodine vapors are a general unspecific color. Chromatogram of 10 essential oils, Stained with vanillin reagent. Specific color reagents exist into which the TLC plate is dipped or which are sprayed onto the plate. Once visible, the R f value of each spot can be determined

8 THIN LAYER CHROMATOGRAPHY Calculation of Rf s R f (A) = 2.0 cm 5.0 cm = 0.40 Solvent Front Distance solvent migrated = 5.0 cm Distance A migrated = 3.0 cm 4.0 cm R f (B) = R f (C) = 3.0 cm 5.0 cm 0.8 cm 5.0 cm = 0.60 = 0.16 Distance B migrated = 2.0 cm 3.0 cm R f (D) = 4.0 cm = cm Origen Distance C migrated = 0.8 cm x x x x A B U C The R f is defined as the distance the center of the spot moved divided by the distance the solvent front moved (both measured from the origin) x D 0.8 cm R f (U 1 ) = 3.0 cm 5.0 cm 0.8 cm R f (U 2 ) = 5.0 cm = 0.60 = 0.16

9 THIN LAYER CHROMATOGRAPHY Calculation of Rf s R f (A) = 2.0 cm 5.0 cm = 0.40 Solvent Front Distance solvent migrated = 5.0 cm Distance A migrated = 3.0 cm 4.0 cm R f (B) = R f (C) = 3.0 cm 5.0 cm 0.8 cm 5.0 cm = 0.60 = 0.16 Distance B migrated = 2.0 cm 3.0 cm R f (D) = 4.0 cm = cm Origen Distance C migrated = 0.8 cm x x x x A B U C x D The R f is defined as the distance the center of the spot moved divided by the distance the solvent front moved (both measured from the origin) 0.8 cm R f (U 1 ) = 3.0 cm 5.0 cm 0.8 cm R f (U 2 ) = 5.0 cm = 0.60 = 0.16

10 THIN LAYER CHROMATOGRAPHY R f s R f values can be used to aid in the identification of a substance by comparison to standards. The R f value is not a physical constant, and comparison should be made only between spots on the same sheet, run at the same time. Two substances that have the same R f value may be identical; those with different R f values are not identical.

11 Absorption of Solutes THIN LAYER CHROMATOGRAPHY R f s The adsorption strength of compounds increases with increasing polarity of functional groups, as shown below: -CH=CH 2, -X, -OR, -CHO, -CO 2 R, -NR 2, -NH 2, -OH, -CONR 2, -CO 2 H. (weakly adsorbed) (strongly adsorbed) (nonpolar) (more polar) Elution Strength of Mobile Phase (ε) Elution strength is generally considered to be equivalent to polarity. A solvents elution strength depends on Intermolecular Forces between the solvent and the analytes and between the solvent and the stationary phase. A more polar (or more strongly eluting solvent) will move all of the analytes to a greater extent, than a less polar, weakly elution solvent. For example, the elution strength of hexane is very low; ε = the elution strength of ethyl acetate is higher; ε = 0.45 the elution strength of ethanol is even higher; ε = 0.68

12 Solvent Properties and Elution Strengths Solvent Hexane CH 3 (CH 2 ) 4 CH 3 MF MW C 6 H Bp ( o C) Density (g/ml) Hazards* Dipole Elution Stength (ε) Flammable Toxic Toluene C 6 H 5 CH 3 C 7 H Flammable Toxic Diethyl ether CH 3 CH 2 OCH 2 CH 3 C 4 H 10 O Flammable Toxic, CNS Depressant Dichloromethane CH 2 Cl 2 CH 2 Cl Toxic, Irritant Cancer suspect Ethyl Acetate CH 3 CO 2 CH 2 CH 3 C 4 H 8 O Flammable Irritant Acetone CH 3 COCH 3 C 3 H 6 O Flammable Irritant Butanone CH 3 CH 2 COCH 3 C 4 H 8 O Flammable Irritant Butanol CH 3 CH 2 CH 2 CH 2 OH C 4 H 10 O Flammable Irritant Propanol CH 3 CH 2 CH 2 OH C 3 H 8 O Flammable Irritant Ethanol CH 3 CH 2 OH C 2 H 6 O Flammable Irritant Methanol CH 3 OH CH 4 O Flammable Toxic Water HOH H 2 O >1

13 Elution Strength of Mixed Solvents The elution strength of the mixture is assumed to be the weighted average of the elution strengths of the components: ε o net = ε o A (mole % A) + ε o B (mole % B) where: mole % A = (moles A) / (moles A + moles B) Thus, to determine the ε o net of a solvent mixture, the molar ratio of the solvents must first be calculated. For example, the ε o net of a solvent mixture prepared from 1.0 ml of ethyl acetate plus 9.0 ml of hexanes is calculated as shown below: ε o net = ε o EtOAc [(moles EtOAc)/(moles EtOAc+moles hexane)] + ε o hexane [(moles hexane)/(moles EtOAc+moles hexane)] where: moles EtOAc = [(volume EtOAc) (density EtOAc)] / [molecular weight of EtOAc] thus: ε o net = {0.45[(1.0mLEtOAc)(0.902g/mL)/(88.11g/mole)]+0.01[(9.0mLhexane)(0.659g/mL)/86.18g/mole)]} {(1.0 mletoac)(0.902g/ml)/88.11g/mole) + (9.0 mlhexane)(0.659g/ml)/86.18g/mole)} and ε o net = 0.067

14 Resolution The separation between two analytes on a chromatogram can be expressed as the resolution, Rs and can be determined using the following equation: Rs = (distance between center of spots) (average diameter of spots) In TLC, if the Rs value is greater than 1.0, the analytes are considered to be resolved. x x

15 Improving Resolution: For two closely migrating components, optimum resolutions are usually obtained when the R f s of both compounds are between 0.2 and 0.5 * To Improve Rs, change the elution strength of the solvent to optimize R f s change ε o net (= in capacity factor), all compounds will be effected similarly. Alter the composition of the solvent system so that the components affinity for the mobile phase vs. the solid phase are differentially changed (= change in selectivity). Changing the chemical nature of the solvent system, such as changing a hydrogen bonding solvent to a solvent which cannot hydrogen bond to the analyte, is often the most effective. ** Improve Rs by decreasing the diameter of the analyte spots. This can be achieved by applying smaller and less concentrated spots. TLC/TLCprocedure.html

16 Optimize R f s

17 TLC Stationary Phases

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19 PREPARATIVE TLC (PTLC)

20 TLC - Optimizing for column chromatography Optimum: 0.2 < R f < 0.5