LTQ Velos a jeho kombinace s FT-MS Orbitrap Je něco převratně nového v iontových pastech? Petr Verner. ThermoFisher Scientific, Praha

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1 The world leader in serving science LTQ Velos a jeho kombinace s FT-MS Orbitrap Je něco převratně nového v iontových pastech? Petr Verner ThermoFisher Scientific, Praha

2 LTQ Velos Co je nového? Hardware HESI II probe standard S-Lens ion optics Dual Cell Linear Trap Prakticky? 2x Rychlejší skenování Zvýšená citlivost Lepší statistika Vyšší rozlišení 2

3 Customer value Hardware features Benefit Customer Value Dual Cell Linear Trap S-Lens HESI II probe 1.Higher Isolation efficiency 2.Higher CID efficiency 3.Faster scan speed 4.Higher resolution Increased ion flux Higher sensitivity Lower fill time Better sample desolvation Higher sensitivity Less solvent noise increased sample IDs Low-level analyte detection Increased Robustness Low-level analyte detection The increased sensitivity and decreased cycle time of the LTQ Velos allow greater depth of sample analysis resulting in increased protein coverage and enhanced access to MS n structural elucidation on a chromatographic time scale. 3

4 LTQ Velos What s new? Source changes HESI II standard Nový design sweep cap Stejné jako na TSQ Vantage 4

5 Dual Cell Linear Ion Trap Configuration No Tube Lens or Skimmer Front Lens Center Lens Detector 1 Back Lens Octopole HPC HPT LPC Detector 2 5

6 LTQ Velos S-Lens podobné jako na TSQ Vantage 6

7 LTQ Velos What s new? Zahnutý Q 7

8 LTQ Velos It has 2 linear traps 8

9 High Pressure Low Pressure High Trapping Efficiency High Dissociation Efficiency High Isolation Efficiency Higher Resolution/Scan Rate Electrospray Source Square Quadrupoles 5. x1-3 Torr He 3.5x1-4 Torr He Ion Transfer Tube Front Lens Center Lens Detector 1 Back Lens Octopole HPC HPT LPC SRIG Inter-multipole Lens 1 Split Gate Lens Detector 2 9

10 Ion Trap Scan Function - MS n Ion Injection Ion Isolation Ion Dissociation Mass Analysis n-1 1 1

11 TIC Motivation 1: Trapping Efficiency He Pressure Effects - TIC Standard Pressure ~6% Trap Eff. Higher Pressure >95% Trap Eff He Pressure (mtorr) 11

12 CID Efficiency Motivation 2: CID Efficiency 1 He Pressure Effects CID Efficiency for MRFA (m/z 524, 3 ms) 8 6 Higher Pressure >8% CID Eff. 4 Standard Pressure ~68% CID Eff He Pressure (mtorr) 12

13 Peak Width (amu) Motivation 3: Resolution/Scan Rate 1.8 He Pressure Effects - Peak Width at Scan Rate 16,667 amu/sec Standard Pressure.55 amu FWHM Lower Pressure <.4 amu FWHM He Pressure (mtorr) 13

14 FWHM (amu) Motivation 3: Resolution/Scan Rate.7.6 Effect of Scan Rate on Peak Width (Res. Ej. Optimized) Current Scan Rate Scan Rate (amu/sec) 2x Current Scan Rate Scan Rate Range 1 Scan Rate Range 2 Scan Rate Range 3 14

15 LTQ Velos dobrá, ale ještě něco nového? Nový RF system Nový Digital board Nový Source Board Nová geometrie lineárních pastí Nová Transfer ion optics Krátce: zvenku může připomínat staré dobré LTQ, ale uvnitř je mnoho novinek 15

16 LTQ Velos Co tedy přináší nového uživateli? Dvojnásobnou rychlost skenování (vyjma TURBO a UltraZoom Skenu) Lepší rozlišení (i v Ultra Zoom skenu) Výrazně zkrácený tzv. Ion injection time Zvýšená citlivost 4-5X 16

17 Scan Speed vs Resolution LCQ Fleet LTQ XL LTQ Velos TurboScan 8, 3 amu FWHM 125, 3 amu FWHM 125, 3 amu FWHM Normal 12,5 amu FWHM 16,7 amu FWHM 33,3 amu FWHM Enhanced 5 amu FWHM 5 amu FWHM 1, amu FWHM ZoomScan 11 amu FWHM 11 amu FWHM 22 amu FWHM Ultra ZoomScan N/A 27 amu FWHM 27 amu FWHM 17

18 Installation Specs LCQ Fleet LTQ XL LTQ Velos Reserpine Concentration 2 ul 1 pg/ul 2 ul 125 fg/ul 2 ul 5 fg/ul Signal to Noise 1:1 1:1 1:1 18

19 Co bude s LXQ? LXQ bude nadále vyráběno jako součást ToxSpec analyzátoru (klinická biochemie) Náhrada HPLC systému Remedy Jinak LCQ Fleet, LTQ XL, LTQ Velos 19

20 LCQ Fleet Exceptional Analytical Value Routine analysis of complex samples Proven MS n performance for compound identification and structural elucidation Familiar platform - provides ease of use Challenges Sample/Process control Reliability Non MS operators Complex Matrices Multi-Component Samples Tools LCQ Fleet Accela Hypersil Gold Columns Metworks Mass Frontier Customer Benefits Established reputation Structural elucidation with MSn Ease of use Greater sensitivity than UV/Single Quad Greater Specificity 2

21 LTQ XL Exceptional coverage at breakthrough speed Fast cycle time, high sensitivity Unmatched MSn spectral quality Platform for hybridization Challenges Post Translational Modification Identification Rapid gradients Complex samples Biological matrices Tools LTQ XL ETD MALDI Accela Hypersil Gold Columns Discoverer Metworks Mass Frontier Customer Benefits Identify the site of PTMs Multiple fragmentation techniques for maximum information Tissue Imaging Solution Excellent quality MSn spectra for library searching Upgradeable to Orbitrap Upgradeable to ETD 21

22 LTQ Velos Balanced 2D linear trap Rapid reliable structural information Ultra-Fast cycle time up to 1Hz The most sensitive full scan MS Standard with HESI Challenges Complex matrices Co-Eluting compounds Post Translation Modification Early phase metabolite ID and Quan Tools LTQ Velos ETD Dual Pump Metworks Mass Frontier Discoverer Customer Benefits Fast cycle time for identifying coeluters Simultaneous Quan and Qual Excellent quality MSn spectra on a u-hplc time scale Data Dependant Decision Tree provides optimum fragmentation Upgradeable to Orbitrap Velos Upgradeable to ETD 22

23 MALDI v kombinaci s LTQ MALDI bude dostupné pouze s LTQ XL We will continue to work on the software, both control and data processing to improve this product further. MALDI will NOT be available on the LTQ Velos Most of the LTQ Velos improvement are due to the source and optics which are removed for MALDI 23

24 The world leader in serving science Alprazolam in Plasma

25 Alprazolam LTQ Velos Plasma with IS 5 orders of Dynamic range LOQ 4fg on column Area Ratio Alprazolam Y = *X R^2 =.9963 W: 1/X Alprazolam Y = *X R^2 =.9963 W: 1/X pg on column pg on column 25

26 Alprazolam LTQ Velos Plasma with IS pg on column LTQ XL (%RSD) LTQ Velos (%RSD)

27 Relative Abundance Relative Abundance LTQ XL vs. LTQ Velos 4fg on column RT: RT: SM: 7G SM: 7G Time (min) 1 scans across the peak 3 scans across the peak Time (min) 27

28 The world leader in serving science Multi Component Quantification

29 Area Ratio Area Ratio Area Ratio 4 Compound Quan LTQ Velos IS (5 scan events) Codeine Y = *X R^2 =.9968 W: 1/X 4.5 Amphetamine Y = *X R^2 =.9969 W: 1/X pg on column Methamphetamine Y = *X R^2 =.9946 W: 1/X pg on Column pg on column Lidocaine Y = *X R^2 =.9966 W: 1/X pg on Column

30 4 Compound Quan LTQ Velos IS (5 scan events) Level Codeine Lidocaine Amphetamine Methamphetamine pg on Column %RSD %RSD %RSD %RSD

31 4 Compound Quan LTQ XL IS (5 scan events) Level Codeine Lidocaine Amphetamine Methamphetamine pg on Column %RSD %RSD %RSD %RSD

32 4 Compound Quan LTQ Velos IS (5 scan events) 4pg on column - No Smoothing RT: Amphetamine Amphetamine-d6 Methamphetamine Lidocaine Codeine Time (min) 32

33 4 Compound Quan LTQ XL IS (5 scan events) 4 pg on column - No Smoothing RT: Amphetamine Amphetamine-d Relative Abundance1 Methamphetamine Lidocaine Codeine Time (min) 33

34 The world leader in serving science Source Ruggedness

35 Peak Area LTQ Velos Ruggedness 6 injections singly crashed plasma 4 2 pg on Column of Alprazolam in Crashed Plasma % RSD with no internal standard Number of injections 35

36 The world leader in serving science LTQ Velos Malathion

37 LTQ Velos Full Scan MS2 1ppb Malathion The Full Scan Solution to Chemical Noise Relative Abundance XIC m/z 99 Fragment XIC m/z 285 Fragment RT: SM: 7G RT: NL: 4.78E1 m/z= F: ITMS + c ESI Full ms @cid [9.-35.] MS Genesis 9 MalathionQuan_39 85 SM: 7G AH: 3977 SN: 44 NL: 4.1 m/z= 2 ITMS [9. Genes Malath Time (min) Time (min) 37

38 Area Calibration Curve.1 to 2 ppb Malathion 6 Malathion Y = *X R^2 =.994 W: 1/X ppb 38

39 .1 ppb Malathion in 5X Diluted QuEChERS Extract S/N E3 39

40 The world leader in serving science Proteomics

41 Increase in Identification for the most complex mixtures Increase in Protein ID 5 ng Increase in Unique Peptide ID 5 ng Increase in Protein ID for LTQ Vs. Velos (5 ng) Increase in Unique Peptide ID for LTQ Vs. Velos (5 ng) % 55% % 53% LTQ LTQ Velos 2 15 LTQ LTQ Velos '6 min' '18 min' '6 min' '18 min' 41

42 Sensitivity for Low Level Injections (2 ng) Increase in ID LTQ Vs. LTQ Velos 2 ng (C. Elegans) % 1 124% 3 2 Series1 Series LTQ XL LTQ Velos 1 2 Proteins Unique Peptides 42

43 The world leader in serving science Metabolism

44 Maropitant Dog Sample 6 min incubation on LTQ XL RT: Base Peak Chromatogram Parent +O -CH2 +2O Time (min) NL: 2.21E7 Base Peak F: ITMS + c ESI Full ms [3.-7.] MS Dog6min2_MS3Top5_LTQ NL: 2.21E7 Base Peak m/z= F: ITMS + c ESI Full ms [3.-7.] MS Dog6min2_MS3Top5_LTQ NL: 3.52E6 Base Peak m/z= F: ITMS + c ESI Full ms [3.-7.] MS Dog6min2_MS3Top5_LTQ NL: 1.63E6 Base Peak m/z= F: ITMS + c ESI Full ms [3.-7.] MS Dog6min2_MS3Top5_LTQ NL: 2.E5 Base Peak m/z= F: ITMS + c ESI Full ms [3.-7.] MS Dog6min2_MS3Top5_LTQ 44

45 Maropitant Dog Sample 6 min incubation on LTQ Velos ~ 1X Higher signal RT: Base Peak Chromatogram Parent +O -CH2 +2O Time (min) NL: 2.9E8 Base Peak F: ITMS + c ESI Full ms [3.-7.] MS Dog6min2_Top5MS3_Velos NL: 2.9E8 Base Peak m/z= F: ITMS + c ESI Full ms [3.-7.] MS Dog6min2_Top5MS3_Velos NL: 2.1E7 Base Peak m/z= F: ITMS + c ESI Full ms [3.-7.] MS Dog6min2_Top5MS3_Velos NL: 1.15E7 Base Peak m/z= F: ITMS + c ESI Full ms [3.-7.] MS Dog6min2_Top5MS3_Velos NL: 1.57E6 Base Peak m/z= F: ITMS + c ESI Full ms [3.-7.] MS Dog6min2_Top5MS3_Velos 45

46 Souhrn The LTQ Velos is 5X more sensitive than the LTQ XL The LTQ Velos is 2X faster than the LTQ XL The LTQ Velos can scan at 1Hz At low concentrations the LTQ Velos gets twice the number of unique peptides and protein identified as an LTQ XL LTQ Velos can be upgraded to ETD and Orbitrap Excellent Simultaneous Quan and Qual instrument High quality data (more samples identified in less time) Low level compound detection HESI II S-Lens Dual Cell Linear Trap Reliability 46