Europass Curriculum Vitae

Transcription

1 Europass Curriculum Vitae Personal information First name(s) / Surname(s) Address Rua da Bélgica, n.25 4ºDto, Carcavelos (Portugal) Telephone(s) Mobile (s) mariasampaiocristovao@gmail.com Nationality Portuguese Date of birth 20 September 1976 Gender Desired employment / Occupational field Female Scientific Researcher Work experience Dates 15/09/2005 Professor Teaching several subjects to degree students, such as: Molecular Biology, Microbiology, Introduction to Biotechnology, Genetic Engineering, Bioinformatic, Biochemistry, Project in Biotechnology, Cellular Biology, Methods in Analytic Chemistry Teaching in Master Programs: MEIO Master (Integrated Studies of the Oceans), Master in Marine Resources, Master in Aquaculture Course Coordinator - Marine Biology and Biotechnology ( ) Course Coordinator - Tourism ( ) Member of the Scientific-Pedagogical Commission from the Marine Biology and Biotechnology Course Member of the Scientific Commission from the School of Tourism and Maritime Technology, ESTM IPL Member of the Marine Resources Research Group Name and address of employer Polytechnic Institute of Leiria - ESTM Campus 4 Santuário de Nossa Senhora dos Remédios, Peniche (Portugal) Higher Education Dates PhD student PhD in Biochemical Engineering and Biotechnology, area of Biochemistry, done under the supervision of Professor Maria Helena Dias dos Santos and Professor Winfried Boos. The work was developed in Instituto de Tecnologia Química e Biológica UNL, together with the Faculty of Biology University of Konstanz, Germany; Work plan integrated in the European Project Exploiting New Solutes from Hyperthermophiles for the Preservation of Biomaterials: Cell Factories for the Production of Hypersolutes ; Page 1 / 5 - Curriculum vitae of

2 Name and address of employer Techniques such as nucleic acid purification (DNA and RNA), protein and DNA electrophoreses, cloning and construction of plasmid vectors, construction of metabolic altered strains, protein purification, chromatography, identification and quantification of compounds by Nuclear Magnetic Resonance (NMR), synthesis of radioactive ( 14 C) compounds, culture of mesophilic, thermophilic and hyperthermophilic organisms, were developed under the PhD work plan. Universidade Nova de Lisboa (University of Lisbon) - ITQB Research Center Dates Research Student Working in a research group, in order to obtain scientific training in research projects and in science and technology institutions. Projects: "Strategies for Survival of Strictly Anaerobic Bacteria in environments containing oxygen" This work involved the development of growth techniques for sulphate reducing bacteria, purification and characterization of reserve polymers, metabolism and enzyme characterization. Towards the Utilization of Cell-Reactors for the Production of Mannosylglycerate, an Enzyme Stabilizer from Hyperthermophiles The final objective was the identification and utilization of compatible solutes as stabilizers of biomaterials, namely enzymes, proteins, vaccines, cells and animal tissues. New compatible solutes from thermophiles and hyperthermophiles: screening, identification and physiological role Name and address of employer UNL - ITQB Research Center Education and training Dates /2005 PhD Thesis Title: Engineering Escherichia coli for the Synthesis of Mannosylglycerate, a solute widely distributed in (hyper)thermophiles. Transport, Metabolism and Physiological Role PhD in Biochemical Engineering and Biotechnology, area of Biochemistry, done under the supervision of Professor Maria Helena Dias dos Santos and Professor Winfried Boos. The work was developed in Instituto de Tecnologia Química e Biológica UNL, together with the Faculty of Biology University of Konstanz, Germany Biochemistry, Microbiology, Biotechnology, Genetic Engineering, Molecular Biology Name and type of organisation UNL - ITQB (Institute of Lisbon University) ISCED 8 Dates 09/ /1998 Degree in Microbiology Biology, Chemistry, Biotechnology, Genetics, Analytical Methods, Molecular Biology, Thermodinamics, Page 2 / 5 - Curriculum vitae of

3 Name and type of organisation Escola Superior de Biotecnologia - UCP (University) Rua Dr. António Bernardino de Almeida, Porto (Portugal) ISCED 5 Name and type of organisation Personal skills and competences Other language(s) Dates Superior Technical Health, Safety and Health at Work Design and management training; legislation, regulations and standards; organizational management; social psychology at work; techniques of information, communication and negotiation; prevention management; occupational hygiene; ergonomics; safety; risk assessment; risk control; organization of emergency; audits. TÜV Akademie Rheinland (Certification Center) Arquiparque, Edifício Zenith. Rua Dr. António Loureiro Borges, 9, 3º, Miraflores (Portugal) 544 h course Self-assessment Understanding Speaking W r i t i n g European level (*) Listening Reading Spoken interaction Spoken production English C2 Proficient user C2 Proficient user C2 Proficient user C2 Proficient user C1 Proficient user French B1 Independent user B1 Independent user A2 Basic User A1 Basic User A1 Basic User Spanish / Castilian B1 Independent user B1 Independent user A1 Basic User A1 Basic User A1 Basic User (*) Common European Framework of Reference (CEF) level Social skills and competences Organisational skills and competences Technical skills and competences Computer skills and competences Artistic skills and competences Driving licence(s) Additional information Team spirit. Ability to work in various types of team projects. Good ability to adapt to multicultural environments. Good communication skills. Sense of organisation and plan elaboration. Good knowledge of higher education burocratic procedures. Large experience in evaluating curricular plans from several degrees. Organisation of seminars. Team organisation. Good knowledge of microbiological, biotechnological and molecular biology technics. Competent with most Microsoft Office programmes. Familiar with Bioinformatic tools. Photo, crafts. B PUBLICATIONS Noronha M, Gerbelová H, Faria TQ, Sampaio MM, Rudolph R, Maçanita AL, Santos H. Enhancing the fluorescence of tyr-59 in ubiquitin by blocking proton transfer. (2009) Phys Chem Chem Phys. 14;11(18): Page 3 / 5 - Curriculum vitae of

4 Jorge D. C, Sampaio M-M., Hreggvidsson G. Ó., Kristjánson J. K. & Santos H. A highly thermostable trehalase from the thermophilic bacterium Rhodothermus marinus. (2007) Extremophiles, 11(1): Silva Z, Sampaio MM, Henne A, Böhm A, Gutzat R, Boos W, da Costa MS, Santos H. The highaffinity maltose/trehalose ABC transporter in the extremely thermophilic bacterium Thermus thermophilus HB27 also recognizes sucrose and palatinose. (2005) J Bacteriol. 187(4): Sampaio MM, Chevance F, Dippel R, Eppler T, Schlegel A, Boos W, Lu YJ, Rock CO. Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mnga (hrsa) gene and is regulated by the mngr (farr) gene product acting as repressor. (2004) J Biol Chem. 13;279(7): Sampaio MM, Santos H, Boos W. Synthesis of GDP-mannose and mannosylglycerate from labeled mannose by genetically engineered Escherichia coli without loss of specific isotopic enrichment. (2003) Appl Environ Microbiol. 69(1): PROCEEDINGS OF INTERNATIONAL MEETINGS Mendonça E, Cruz A, Neves-Afonso C, Sampaio M (2008) Genetic characterization through RAPD of Armeria spp. from Berlengas Natural Park and Peniche coast. ESBES 7-7th European Symposium on Biochemical Engineering Science, Universidade do Algarve, Faro, 7-10 September Mendes MJ, Lopes N, Capitão A, Gomes D, Cruz A, Mouga TM, Neves C, Sampaio M-M (2007) Germoplasm bank construction and genetic characterization of the endemic flora from Berlengas Natural Park through RAPD. Journal of Biotechnology, 131: S32 Pires V, Matos I, Cruz A, Mouga TM, Sampaio M-M, Leandro S, Neves C (2007) Micropropagation of selected native plants from Peniche area. Journal of Biotechnology, 131: S33 POSTERS Barros I, Duarte MM, Elias L, Cruz A, Afonso C, Pedrosa R, Sampaio MM (2009). Evaluating Bioactive Compounds extracted from Anemones living on the Peniche Coast and on Berlengas Natural Reserve. International Meeting on Marine Resources, Peniche, November. Pinteus S, Alves C, Rodrigues AI, Azevedo S, Sampaio MM, Afonso C, Cruz A, Mouga T, Pedrosa R (2009). High antioxidant potential of Fucus spiralis and Enteromorpha compressa extracts collected from Peniche Coast (Portugal). International Meeting on Marine Resources, Peniche, November. Pinteus S, Azevedo S, Alves C, Rodrigues AI, Mouga T, Cruz A, Afonso C, Sampaio MM, Pedrosa R (2009). High antioxidant potential of Fucus spiralis extracts collected from Peniche coast (Portugal). 14th European Congress on Biotechnology, Barcelona, Spain September. Silva KJ, Santos L, Mendonca E, Cruz A, Sampaio MM, Mouga T, Afonso C (2009). In-vitro micropropagation of Pulicaria microcephala, an endemic species from Berlengas Natural Park. International Meeting on Marine Resources, Peniche, November. Mendonça E, Cruz A, Neves-Afonso C, Sampaio M (2008) Genetic characterization through RAPD of Armeria spp. from Berlengas Natural Park and Peniche coast. ESBES 7-7th European Symposium on Biochemical Engineering Science, Universidade do Algarve, Faro, 7-10 September Page 4 / 5 - Curriculum vitae of Mendes MJ, Lopes N, Capitão A, Gomes D, Cruz A, Mouga T, Neves C, Sampaio M-M (2007) Germoplasm bank construction and genetic characterization of the endemic flora from Berlengas Natural Park through RAPD. 13th European Congress on Biotechnology, Barcelona, Spain September

5 Pires V, Matos I, Cruz A, Mouga T, Sampaio M-M, Leandro S, Neves C (2007) Micropropagation of selected native plants from Peniche area. 13th European Congress on Biotechnology, Barcelona, Spain, September. Jorge C, Sampaio M-M, Hreggvidsson G, Kristjánson J, Santos H (2006) A highly thermostable trehalase from the thermophilic bacterium Rhodothermus marinus. Extremophiles 2006 International Conference, Brest, France, September. Sampaio M-M., Silva Z., da Costa M.S., Boos W. & Santos H. Transport of Mannosylglycerate and Trehalose in the Thermophilic Bacterium Thermus thermophilus. 4th International Congress on Termophiles, Exeter, UK, Sampaio M-M., Santos H. & Boos W. Synthesis of GDP-Mannose and Mannosylglycerate by Engineered Escherichia coli without Loss of Specific Activity. 4th International Congress on Extremophiles, Naples, Italy, Jorge C., Sampaio M-M., Mingote A., Boos W. & Santos H. Trehalose Metabolism in Rhodothermus marinus: chraterization of a very active periplasmic trehalase. 4th International Congress on Extremophiles, Naples, Italy, Jorge C., Sampaio M-M., Boos W. & Santos H. Trehalose and Glucose Metabolism in the Thermophilic Bacterium Rhodothermus marinus. Micro 2001, Congresso Nacional de Microbiologia, Sampaio M-M., Fareleira P., Boos W. & Santos H. The substrate specificity of Glycogen Synthase from Desulfovibrio gigas could explain the lack of regulation in glycogen biosynthesis. BIOQUIMICA 2000, XII Congresso Nacional de Bioquímica, Póvoa do Varzim, ORAL COMMUNICATIONS Pinteus S, Monteiro H, Araujo E, Duarte L, Felicio L, Sampaio MM, Afonso C, Cruz A, Mouga T, Pedrosa R (2009). High antimicrobial potential of methanol extracts of Sphaerococcus coronopifolius and Plocamium cartilagineum collected from the Peniche Coast (Portugal). International Meeting on Marine Resources 09, Peniche, Portugal, Novembro Sampaio M-M. Engineering Escherichia coli for the production of Mannosylglycerate. ITQB Boos W., Sampaio M-M., Mannosylglycerate transport in E. coli. 5th European Project Meeting Hypersolutes, March Sampaio M-M. The use of 14 C-compounds to study osmoadaptation in thermophiles. Gulbenkian Institute of Science, Oeiras, October Sampaio M-M. Synthesis of mannosylglycerate in engineered E. coli cells. 3rd European Project Meeting Hypersolutes, March Eppler T., Sampaio M-M., Horlacher R., Boos W. Construction of an expression system for Bacillus stearothermophilus. 3rd European Project Meeting Hypersolutes, March Sampaio M-M. Optimisation of MG synthesis in E. coli: in the search for a MG synthase with optimal activity in the mesophilic range of temperature. 4th European Project Meeting Hypersolutes, September Page 5 / 5 - Curriculum vitae of