Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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1 Schedule of ccreditation United Kingdom ccreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK LS Food and Pharmaceutical Issue No:098 Issue date: 02 September 2016 ccredited to Sands Mill Huddersfield Road Mirfield West Yorkshire WF14 9DQ Contact: lexia Masters Tel: +44 (0) Fax: +44 (0) alexia.masters@ alsglobal.com Website: Laboratory locations: below s covered by the organisation and their relevant activities details ctivity code ddress Medcalfe Way Bridge Street Chatteris Cambridgeshire PE16 6QZ Local contact dam Rush Tel: +44 (0) Fax: +44 (0) adam.rush@alsglobal.com Website: Chemical Microbiological roup Co-ordination ctivities ddress Francis House Lords Way Kingmoor Business Park Kingstown Carlisle Cumbria C6 4SL Local contact Ian O Mahony Tel: +44 (0) Fax: +44 (0) ian.o mahony@alsglobal.com Website: Microbiological (Food and Water) B ddress First Floor Building 550 Winch Road Kent Science Park Sittingbourne Kent ME9 8EF Local contact drian oldsmith Tel: +44 (0) Fax: +44 (0) adrian.goldsmith@alsglobal.com Website: Microbiological (Food and Water) C ddress 2 Bartholemews Walk Cambridgeshire Business Park Ely Cambridgeshire CB7 4ZE Local contact Sharon Hanly Tel: +44 (0) Fax: +44 (0) sharon.hanly@alsglobal.com Website: Chemical (Pharmaceutical) Microbiological (Pharmaceutical) E ssessment Manager: LS2 Page 1 of 34
2 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical details ctivity code ddress Sands Mill Huddersfield Road Mirfield West Yorkshire WF14 9DQ Local contact Hannah Mclister Tel: +44 (0) Fax: +44 (0) Website: Microbiological (Food) Chemical (Food) F ddress 10 Sandown Centre White Horse Business Park Trowbridge B14 0XD Local contact Margaret Oulsnam Tel: +44(0) Fax: +44(0) Margaret.oulsnam@alsglobal.com Website: Microbiological (Food and water) ssessment Manager: LH Page 2 of 34
3 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical DETIL OF CCREDITTION NIML FEEDINSTUFFS, FOOD ND FOOD PRODUCTS (restricted matrices specified as appropriate) Chemistry Tests Nutritional and eneral Chemistry Documented In-House Methods: cidity M/C/922 by Titration flatoxins B1, B2, 1, 2 and Total flatoxins flatoxin M1 M/R/999 using HPLC-immunoaffinity M/R/993 using HPLC-immunoaffinity mino cid Profile: Total M/V/206 using HPLC sh M/C/803 based on BS 4401:Part 1:1998 and Feeding Stuffs (Sampling and nalysis) Regulations 2010 (excluding Cheese) Benzoic acid/sodium benzoate Sorbic acid/potassium sorbate M/V/757 Carbohydrate: available, by difference total, by difference Starch, by difference By calculation: M/C/901 using the Food Labelling Regulations 1984 (SI No 1305) and Feedingstuffs Regulations 2005 Chloride M/C/603 using Potentiometric titration based upon JOC 1974 Cholesterol M/V/114 using as Chromatography JOC 1993 Energy M/C/901 by calculation using Commission Regulation (EC) 2008/100 and 90/496 ssessment Manager: LH Page 3 of 34
4 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical NIML FEEDINSTUFFS, FOOD ND FOOD PRODUCTS (restricted matrices specified as appropriate) (cont d) Chemistry Tests (cont d) Nutritional and eneral Chemistry (cont d) Fat (oil) 1) M/C/101 using Soxhlet, Method based on Feeding Stuffs (Sampling and nalysis) Regulations ) M/C/102 using acid hydrolysis, Method B based on Feeding Stuff Regulations ) M/C/103 based on Werner-Schmidt Method BS 4401:Part 4:1970 4) M/C/104 using mixed solvents based on Bligh and Dyer Method Fatty cid Composition/Profile - Saturated fats - Monounsaturated fats - Polyunsaturated fats - cis:trans analysis - non-normalised analysis - % actual fatty acids M/C/107 using as Chromatography based on BS EN ISO :2011 Free Fatty acid M/C/108 based on BS EN ISO 660:2009 Fibre: (nimal feedingstuffs) 1) Crude M/C/300 using fibrecap method - digestion 2) Dietary M/C/302 based on Englyst method 3) Dietary M/C/309 based on OC Method No Hydroxyproline and Collagen M/C/998 using Colorimetry BS 4401:Part 11:1995 ssessment Manager: LH Page 4 of 34
5 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical NIML FEEDINSTUFFS, FOOD ND FOOD PRODUCTS (restricted matrices specified as appropriate) (cont d) Chemistry Tests (cont d) Nutritional and eneral Chemistry (cont d) (Food Products) luten/liadin content: quantitative M/R/253 by R5 Mendez Extraction and ELIS technique (Food products and environmental swabs) Quantitative (foods) and qualitative (swabs) determination of Casein and Caseinates MR/R/254 by ELIS using graquant kit (Excluding samples containing more than 20% Chicken) Quantitative determination of Egg white protein (ovomucoid) Quantitative determination of Soya (soya trypsin inhibitor) MR/R/255 by ELIS using Imutest kit MR/R/256 by ELIS using Imutest kit (Food products, processed and unprocessed, and environmental swabs) Quantitative determination of Peanut Quantitative determination of Mustard MR/R/257 by ELIS using Imutest kit MR/R/258 by Elisa using Imutest kit (nimal feedingstuffs) Metabolisable Energy (Hartel) By calculation: M/C/901 Feedingstuffs Regulations 2005 (Fish and Meat Products) Meat Content: Lean Total By calculation: M/C/902 by Stubbs and Moore Food and Feed samples for samples containing more than 20% moisture and less than 25% sugar. Excluding herbs and spices and samples containing alcohol Moisture content M/C/1017 by microwave drying Moisture Content M/C/801 based on Feeding Stuff Regulations 2000 Ochratoxin M/R/960 HPLC - Fluorescence detection with immunoaffinity columns ssessment Manager: LH Page 5 of 34
6 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical NIML FEEDINSTUFFS, FOOD ND FOOD PRODUCTS (restricted matrices specified as appropriate) (cont d) Chemistry Tests (cont d) Nutritional and eneral Chemistry (cont d) (Beverages) Ethanol M/V/917 using as Chromatography Peroxide Value M/C/112 BS :2001 Nitrogen/Protein M/C/224, by DUMS technique ph ESM-C014 using ph meter (nimal Feeds and Cereal Based Foods) Starch M/C/401 using Polarimetric technique based on EC Directive 79/1999/EC Sugars - Totals M/C/403 based on Luff-Schoorl Method and Feeding (Sampling and nalysis) Regulations 2010 (Food products and pure sugars) Sugars Total and Individual: Fructose alactose lucose Lactose Maltose Sucrose M/C/1014 by Ion exchange chromatography Sulphur dioxide M/C/925 based on Monier Williams procedure Tryptophan, Total M/V/228 using HPLC based on Official Journal of the European communities, directive EC (Meat and meat Products) Water: dded By calculation: M/C/902 Meat Products and Spreadable Fish Products Regulations 1984 (SI No 1566) Total Fat and Moisture M/C/1015 by oven drying and Pulsed NMR ssessment Manager: LH Page 6 of 34
7 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical NIML FEEDINSTUFFS, FOOD ND FOOD PRODUCTS (restricted matrices specified as appropriate) (cont d) Chemistry Tests (cont d) Nutritional and eneral Chemistry (cont d) Metals: Calcium Copper Iron Magnesium Manganese Phosphorus Potassium Sodium Zinc M/C/1002 using ICP-OES (Meat, meat products, brine, vegetables, animal feeds and baby foods) Determination of the nitrite and nitrate content M/V/1004 using high performance anion exchange chromatography based on BS EN :2005 Pesticide Residues: Dithiocarbamates (as CS 2 ) M/R/240 using CMS (Fish and Meat Products) Ivermectin M/R/206 using HPLC (Fruits, vegetables, fungi and cereals) (Potatoes, onions, carrots and parsnips) Chlormequat and Mepiquat M/R/231 using LC-MS Maleic Hydrazide M/R/104 using HPLC Multi-residue screen and quantitative analysis: M/R/110 using C-MS cephate ldrin trazine zinphos-ethyl zinphos-methyl zoxystrobin Benalaxyl Bifenthrin Bitertanol Boscalid Bromophos ssessment Manager: LH Page 7 of 34
8 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical NIML FEEDINSTUFFS, FOOD ND FOOD PRODUCTS (restricted matrices specified as appropriate) (cont d) Chemistry Tests (cont d) Pesticide Residues: (cont d) Multi-residue screen and quantitative analysis: (cont d) Bromophos-ethyl Bromopropylate Bromuconazole Bupirimate Buprofezin Cadusafos Carbofuran (parent molecule) Carbophenothion Carbosulfan Chlordane Chlorfenvinphos Chlorothalonil Chlorpropham Chlorpyrifos Chlorpyrifos-methyl Chlorthal-dimethyl Chlorthion Chlozolinate Cyanazine Cyfluthrin Cypermethrin Cyproconazole DDT Deltamethrin Desmetryn Diazinon Dichlofluanid Dichlorvos Dicloran Dicofol Dicrotophos Dieldrin Difenoconazole Dimethoate Dimethomorph Diphenylamine Endosulfan Endrin Ethion Ethofumesate ssessment Manager: LH Page 8 of 34
9 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical NIML FEEDINSTUFFS, FOOD ND FOOD PRODUCTS (restricted matrices specified as appropriate) (cont d) Chemistry Tests (cont d) Pesticide Residues: (cont d) Multi-residue screen and quantitative analysis: (cont d) Ethoprophos Etridiazole Etrimfos Fenarimol Fenchlorphos Fenitrothion Fenpropathrin Fenpropimorph Fensulfothion Fenthion Fenvalerate Fipronil Fluazifop-P-butyl Flucythrinate Flusilizole Fonofos Furalaxyl Furathiocarb Heptachlor Heptenophos Hexachlorobenzene (HCB) Hexachlorocyclohexane-alpha Hexachlorocyclohexane-beta Hexachlorocyclohexanegamma Hexaconazole Imazalil Iodofenfos Iprodione Isazophos Isofenphos Isoprocarb Kresoxim-methyl lambda cyhalothrin Malathion Mecarbam Mepanipyrim Metalaxyl Metalaxyl-M Metazachlor ssessment Manager: LH Page 9 of 34
10 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical NIML FEEDINSTUFFS, FOOD ND FOOD PRODUCTS (restricted matrices specified as appropriate) (cont d) Chemistry Tests (cont d) Pesticide Residues: (cont d) Multi-residue screen and quantitative analysis: (cont d) Methacrifos Methamidophos Methidathion Methoxychlor Metribuzin Mevinphos Monocrotophos Myclobutanil Napropamide Nuarimol Ofurace Omethoate Oxadixyl Paclobutrazol Parathion Parathion-methyl Penconazole Pendimethalin Permethrin Phenthoate Phosalone Phosmet Phosphamidon Pirimicarb Pirimiphos-ethyl Pirimiphos-methyl Prochloraz Procymidone Profenophos Prometryn Propachlor Propargite Propham Propiconazole Propyzamide Prothiofos Pyrazophos Pyridaphenthion Pyrimethanil Quinalphos ssessment Manager: LH Page 10 of 34
11 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical NIML FEEDINSTUFFS, FOOD ND FOOD PRODUCTS (restricted matrices specified as appropriate) (cont d) Chemistry Tests (cont d) Pesticide Residues: (cont d) Multi-residue screen and quantitative analysis: (cont d) Quintozene Simazine Sulfotep Tebuconazole Tecnazene Tefluthrin Terbuthylazine Terbutryn Tetradifon Tetrachlorvinphos Tetramethrin Thiabendazole Tolclofos-methyl Tolyfluanid Triadimefon Triadimenol Trifloxystrobin Triazophos Trifluralin Vinclozolin (parent molecule) Zoxamide FRUIT ND VEETBLES (excluding Dried Fruit) Multi-residue screen and quantitative analysis: M/R/229 by LC-MS/MS using QuEChERS Extraction cettamiprid dicarb ldicarb sulfone ldicarb sulfoxide Bendiocarb Butocarboxim Carbaryl Carbendazim Carbofuran Carbofuran 3-OH Chlorotoluron Cyazofamid Cyprodinil ssessment Manager: LH Page 11 of 34
12 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical FRUIT ND VEETBLES (excluding Dried Fruit) (cont d) Chemistry Tests (cont d) Pesticide Residues: (cont d) Multi-residue screen and quantitative analysis: (cont d) M/R/229 by LC-MS/MS using QuEChERS Extraction Diethofencarb Diuron Ethiofencarb Ethofumesate Fenamidone Fenhexamid Fenoxycarb Fenpropimorph Fenpyroximate Fenuron Imazalil Imidacloprid Iprovalicarb Isoproturon Lenacil Linuron Methabenzthiazuron Methomyl (including thiodicarb) Metoxuron Metsulfuron-methyl Monolinuron Monuron Oxamyl Pencyuron Phorate Propamocarb Propoxur Pyraclostrobin Quinmerac Spinosyn /D (Spinosad), Spiroxamine Tebufenpyrad Thiabendazole Thiacloprid Thiamethoxam Tridemorph Triflusulfuron-methyl ssessment Manager: LH Page 12 of 34
13 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical FOODS and FOOD PRODUCTS Chemistry Tests (cont d) Documented In-House Method High water content; High acid content and high water content; High sugar and low water content; High oil content and intermediate water content; High starch and/or protein content and low water and fat content, Identification, quantification and confirmation of pesticide residues See Table 1 (at end of this schedule for the list of pesticides covered) M/R/1003 QuEChERS extraction with LC-MS/MS and C-MS/MS Detection Fish and Shellfish Malachite reen and Leucomalachite reen M/R/211 using HPLC Sudan red, Para Red and Butter Yellow Dyes M/R/244 using LC-MS/MS DIRY PRODUCTS Chemical/Physical Tests Milk and Cream Phosphatase ESM C007 by the schaffenburg and Mullen (&M) Method F Fat content ESM C011 based on erber method BS 696:Parts 1 and 2:1989 (withdrawn) F Determination of Fat ESMC018 by Rose ottlieb F DIRY PRODUCTS (cont d) Chemical/Physical Tests Salt content ESM C017 (Chloride Meter) F Fat in dry matter (by calculation) ESM-C015/ESM C011 F ph ESM-C014 using ph meter F (Cheese only) Moisture content (and dry matter, by calculation) ESM-C015 (microwave method) F (Cheese and Concentrate Milk samples only) Moisture content (and dry matter, by calculation) ESM-C016 by oven drying F ssessment Manager: LH Page 13 of 34
14 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical NIML FEEDINSTUFFS, Chemistry Tests Documented In-House Method FOOD ND FOOD PRODUCTS, INCLUDIN PREMIXES, SUPPLEMENTS, DRINKS, Vitamin (Retinol) M/V/702 using HPLC PHRMCEUTICL Vitamin E (lpha tocopherol) based on nalyst, PRODUCTS, eg, ugust 1985, based on CPSULES, PSTILLES Volume 11, 110 and Pure ND TBLETS, and pplied Chemistry, 1988, MEDICTIONS (restricted Volume 60, p matrices specified as appropriate) (Excluding Silage) Beta Carotene M/V/906 using HPLC (Excluding pharmaceutical products) Vitamin B3 complex (Nicotinic acid and Nicotinamide) M/V/751 using HPLC VitaminB 1 (Thiamine) Vitamin B 2 (Riboflavin) M/V/703 using HPLC (Excluding pharmaceutical products or drinks) Vitamin B 6 (Pyridoxine) M/V/752 using HPLC Vitamin B 12 (Cyanocobalamin) M/V/754, Biacore Instrumentation (Excluding vegetable products) Vitamin B complex (Free Folic cid) M/V/755 Biacore Instrumentation Vitamin C (scorbic acid) M/V/710 using HPLC (Excluding pharmaceutical products or drinks) Vitamin B 6 (Pyridoxine) M/V/752 using HPLC Vitamin B 12 (Cyanocobalamin) M/V/754, Biacore Instrumentation (Excluding vegetable products) Vitamin B complex (Free Folic cid) M/V/755 Biacore Instrumentation Vitamin C (scorbic acid) M/V/710 using HPLC ssessment Manager: LH Page 14 of 34
15 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical NIML FEEDINSTUFFS, FOOD ND FOOD PRODUCTS, INCLUDIN PREMIXES, SUPPLEMENTS, DRINKS, PHRMCEUTICL PRODUCTS, eg, CPSULES, PSTILLES ND TBLETS, MEDICTIONS (restricted matrices specified as appropriate) Chemistry Tests (cont d) (Premixes, Supplements, Tablets and Pastilles) Vitamin D 2 & D 3 (Cholecalciferol & Ergocalciferol) M/ V/707 using HPLC Vitamin D 2 & D 3 (Cholecalciferol & Ergocalciferol) M/V/723 using HPLC (Premixes, Supplements, Tablets and Raw Materials) Vitamin E (lpha tocopherol acetate) M/V/715 using HPLC Biotin M/V/753 using Biacore instrumentation Molecular Testing Cooked and Raw Meat Products Detection of mitochondrial DN from the following species: species Chicken Cow Horse Pig Sheep Turkey In-house methods M/R/1001 and M/R/1002 using Real Time Polymerase Chain reaction (RT- PCR) ssessment Manager: LH Page 15 of 34
16 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical FOOD and FOOD Microbiological Tests Documented In-House PRODUCTS Methods: (including environmental swabs and dairy as specified as appropriate) Enumeration: Bacillus cereus, presumptive 1) ESM/M/319 using PEMB at 37 0 C for 24 hours and ambient additional 24 hours 2) KM71 based on ISO 7932:2004 using MYP agar, B, C, F, B (Raw and Cooked Poultry meat and Skin) Campylobacter spp (thermotolerant) ESM/M/325 based on ISO/TS :2006, F Clostridium perfringens, presumptive and Clostridium spp ESM/M/310, using TSC agar based on BS EN ISO 7937:2004, B, C, F, Clostridium perfringens, confirmed ESM/M/310, using TSC agar based on BS EN ISO 7937:2004 with confirmation by biochemical tests B (including dairy) Coliforms, presumptive ESM/M/302 based on BS EN ISO 4832:2006 at 37 C for general food and swabs and 30ºC for dairy products, B, C, F, Coliforms and Escherichia coli KM28 Client specified method using chromogenic agar B Enterococci/Faecal Streptococci presumptive ESM/M/314 based on BS :1985, C, F (including nimal By Products) Enterobacteriaceae, presumptive 1) Method ESM/M/303 for the purpose of BPR 2011 based on BS ISO :2004 in accordance with Regulations EC 1069/2009 and 142/2011, 2) Method ESM/M/303 based on BS ISO :2004, B, C, F, ssessment Manager: LH Page 16 of 34
17 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical FOOD and FOOD PRODUCTS (cont d) (including environmental swabs and dairy as specified as appropriate) Microbiological Tests (cont d) Enumeration: (cont d) Enterobacteriaceae, confirmed Method ESM/M/303 based on BS ISO :2004 with confirmation by oxidase and fermentation tests B (including mushroom casings, peat, sugar beet) Escherichia coli (ßglucuronidase positive) 1) ESM/M/304 using TBX pour plate based on BS ISO :2001, B, C, F, 2) ESM/M/305, Client specified (TBX and membrane) based on BS ISO :2001, B, C, F, (including soft drinks) Lactic acid bacteria, presumptive ESM/M/320 based on BS ISO 15214:1998, C, F, Lactic acid bacteria KM43 using MRS agar spread plates incubated microaerophilically at 30 C B Listeria spp ESM/M/321 based on BE EN ISO :1998 (1:2004) and confirmation using ESM/M/531, B, C, F, (including soft drinks) Mesophilic aerobic and anaerobic spores ESM/M/316 Client specified method Pseudomonas species, presumptive ESM/M/312 based on BS :1996 and BD ISO 13720:2010, B, C, F, ssessment Manager: LH Page 17 of 34
18 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical FOOD and FOOD PRODUCTS (cont d) (including environmental swabs and dairy as specified as appropriate) Microbiological Tests (cont d) Enumeration: (cont d) Staphylococcus aureus (Coagulase positive staphylococci), confirmed 1) ESM/M/307 based on BS EN ISO 6888:Part 1:1999 using confirmation as detailed in BS EN ISO6888-3, B, C, F, 2) ESM/M/307 based on BS EN ISO 6888:Part 1:1999 with confirmation by Oxoid Staphytect latex agglutination or by coagulase plasma upon request B (including soft drinks) Total erobic Colony Count: 3 days, 30 C, ESM/M/300 based on BS EN ISO :2013, B, C, F, (including soft drinks) Total erobic Colony Count: 2 days, 30 C ESM/M/300 using PC pour plate techniques at the temperatures and times specified, B, C, F, (including soft drinks) Total erobic Colony Count: 3 days, 22 C 2 days, 37 C 1 days 37 C ESM/M/300 using PC pour plate techniques at the temperatures and times specified, B, C, F, (including soft drinks) Colony counts: 1 day, 37 C 2 days, 30 C 2 days, 37 C 3 days, 22 C ESM/M/100, pour plate using YE based on MDW 20012, Part 7, C, F, Yeasts and Moulds: 5 days at 25 C 1) ESM/M/308 based on BS ISO : days at 22 C 2) ESM/M/315using D18 agar (high sugar products only) based on BS ISO :2008 3) ESM/M/309 client specified method using OYE 4) KM46 client specified method using Phytone Yeast Extract agar, F, B, C, F B, C B ssessment Manager: LH Page 18 of 34
19 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical FOOD and FOOD PRODUCTS (cont d) (including environmental swabs and dairy as specified as appropriate) Microbiological Tests (cont d) Enumeration: (cont d) Detection: (including raw chicken carcass and portion rinse) Campylobacter spp, confirmed ESM/M/500 using Preston Enrichment Broth at 37 o C for 48 hours onto CCD agar at 41.5 o C for 48 hours, F, (including environmental swabs and dairy products, dairy products only at location F) Enterobacteriaceae, Presumptive ESM/M/563 based on BS EN ISO :2004 B, F Escherichia coli O157 1) M/M/382 customer specified method based onbs EN ISO :2001 2) M/M/382 modified in-house method using one selective media at 41.5 C for 18 hours Escherichia coli ESM/M/561 customer specified method based on ISO/TS :2005, B, F, Infant milk formulae (only) Cronobacter (formerly Enterobacter) sakazakii PHRMM011 based on ISO/TS 22964:2006 (E) with confirmation by Biochemical test E Listeria spp and Listeria monocytogenes ESM/M/524 by the Oxoid Precis Method C, (Including Mushroom casings, peat andsugar beet) Listeria monocytogenes and other named Listeria species ESM/M/522 based on ISO :1997 and amendment 2004 using chromogenic agar, C, F, ssessment Manager: LH Page 19 of 34
20 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical FOOD & FOOD PRODUCTS, NIML FEEDS and ENVIRONMENTL SWBS Microbiological Tests (cont d) Detection: (cont d) (excluding milk and milk powders and garlic paste/puree or products containing garlic as the main component) Listeria monocytogenes and other named Listeria species 1) ESM/M/530 based oniso :1997 and amendment 2004 with detection using VIDS B, C (including swabs and dairy) 2) KM61 using BioMerieux VIDS LIS, and confirmation with ESM/M/531 B (including swabs and dairy) 3) KM08 client specified method based on US FD BM method. Supplemented BLEB used throughout and Oxford Listeria selective agar and confirmation with ESM/M/531 B (including swabs and dairy) 4) ESM/M/523 using Solus ELIS kit method and DYNEX equipment, B, C F, Listeria spp and monocytogenes confirmation ESM/M/531 using oxidase catalase gram stain and biochemical tests, B, C, F, Presumptive Listeria culture isolates (from food and environmental samples) Prepared targets (colony smear plus matrix) Confirmation of named Listeria species: L. monocytogenes, L. innocua, L. welshimeri, L.grayi, L.seeligeri and L.ivanovii ESMM532 using the VITEK (V2) MS MLDI TOF instrument, B, C, D,E,F, for isolate prep, submission to and local reporting ssessment Manager: LH Page 20 of 34
21 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical FOOD & FOOD PRODUCTS, NIML FEEDS and ENVIRONMENTL SWBS (cont d) Microbiological Tests (cont d) Detection: (cont d) (including mushroom casings, peat, sugar beet, chocolate and chocolate confectionary, raw chicken carcass and portion rince) Salmonella spp 1) ESM/M/511 based on BS EN ISO 6579:2002 2) ESM/M/518 by the Oxoid Precis Method with confirmation by Biochemical test, B, C, F, C, Salmonella spp 3) ESM/M/516 Easy Vidas C Salmonella confirmation 4) ESM/M/515 using Solus ELIS Kit method and DYNEX equipment 5) KM60 using BioMerieux VIDS SLM, dual selective enrichment, confirmation by BioMerieux PI 20E ESM/M/517 by oxidase, poly O and poly H antisera and biochemical tests (PI 20E), B, C, F, B, B, C, F, (nimal By-Products) Salmonella spp (presumptive) ESM/M/511 for the purpose of BPR 2011 based on BS EN ISO 6579:2002 in accordance with the Regulations (EC) 1069/2009 and 142/2011, Staphylococcus aureus ESM/M/562 based on BS EN ISO , F (including swabs at location B only) Coagulase Positive Staphylococci ESM/M/562 based on BS EN ISO B Vibrio parahaemolyticus ESM/M/551 based on ISO/TS :2007 ssessment Manager: LH Page 21 of 34
22 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical ENVIRONMENTL TMOSPHERIC Microbiological Tests Examination of settle plates WTERS Enumeration of TVC and/or Yeasts and moulds (confirmed) ESM/M/322 using RBC and/or PC, F Raw and potable waters Testing for the purpose of enforcement of the Water Supply (Water Quality) Regulations 2000 [SI 2000/3184]. The testing is in accordance with the Drinking Water Testing Specification (DWTS). Documented In-House Procedures based on/ incorporating procedures in the HMSO publication The Microbiology of Drinking Water - Methods for the Examination of Water and ssociated Materials Coliforms, presumptive and confirmed E coli, presumptive and confirmed ESM/M/107 using membrane filtration onto ML based on the MDW 2009, Part 4B C, Colony counts: 1 day, 37 C 2 days, 30 C 2 days, 37 C 3 days, 22 C ESM/M/100, pour plate using YE based on the MDW 2012, Part 7 C, Enterococci/Faecal streptococci, presumptive and confirmed ESM/M/106 by membrane filtration based on the MDW 2012, Part 5 C, Clostridium perfringens, confirmed ESM/M/105 by membrane filtration using TSC based on the MDW 2010, Part 6 C, Sulphite-reducing Clostridia ESM/M/104 by membrane filtration using TSC based on the MDW 2010, Part 6 C, Pseudomonas aeruginosa, confirmed ESM/M/102 by membrane filtration using CN agar based on the MDW 2010, Part 8B C, ssessment Manager: LH Page 22 of 34
23 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical WTERS Raw, Potable, Bottled Mineral, Recreational, Wastewaters and Process Microbiological Tests (cont d) Enumeration: Documented in House Methods based on The Microbiology of Drinking Water 2002 (relevant sections in parentheses) Coliforms, presumptive Escherichia coli, presumptive ESM/M/107 using ML Based on the MDW 2009 Part 4B, C, F, Coliforms, confirmed Escherichia coli, confirmed ESM/M/107 using ML Based on the MDW 2009 Part 4B, C, Coliforms and E. coli, MPN confirmed KM59 using IDEXX based on the MDW 2009 Part 4B B Colony counts: 2 days, 37 C 3 days, 22 C ESM/M/100, pour plate using YE based on MDW 2007, Part 7, B, C, F, Colony counts: 1 day, 37 C 2 days, 30 C ESM/M/100, pour plate using YE based on MDW 2007, Part 7, C, F, Enterococci/Faecal streptococci, presumptive ESM/M/106 by membrane filtration based on MDW 2012, Part 5 C, F, Enterococci/Faecal streptococci, confirmed ESM/M/106 by membrane filtration based on MDW 2012, Part 5 B, C, Clostridium perfringens, (presumptive) ESM/M/105 by membrane filtration based on MDW 2010, Part 6B F Clostridium perfringens, confirmed ESM/M/105 by membrane filtration based on MDW 2010, Part 6B B, C, Sulphite-reducing Clostridia ESM/M/104 by membrane filtration using TSC based on MDW 2010, Part 6 C, F, Pseudomonas aeruginosa, presumptive ESM/M/102 by membrane filtration using CN agar based on the MDW 2010, Part 8B C, F, ssessment Manager: LH Page 23 of 34
24 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical WTERS Raw, Potable, Bottled Mineral, Recreational, Wastewaters and Process (cont d) Microbiological Tests (cont d) Enumeration (cont d): Documented in House Methods based on The Microbiology of Drinking Water 2002 (relevant sections in parentheses) Pseudomonas aeruginosa, confirmed KM78 by membrane filtration using CN agar based on the MDW 2010, Part 8B B (Raw, potable, recreational (swimming and spa pool), process (chilled and heated closed systems) ground and waste waters Pseudomonas spp, presumptive ESM/M/110 by membrane filtration using PCFC agar incubated at 30 o C for 48 hours C Potable, Recreational, Process, environmental samples, including swabs Isolation of Legionella spp, presumptive. Identification of Legionella spp: Legionella pneumophila sg 1, 2-14 and species BIO 114 based on BS 6068:Section 4.12:1998 C BCTERIL CULTURES Microbiological Tests Identification of: In-House Methods based on the use of nalytical Profile Index (PI Biomerieux) Enterobacteriaceae BIO 082 C Non enteric ram negatives BIO 081 C Listeria spp BIO 083 C ssessment Manager: LH Page 24 of 34
25 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical PHRMCEUTICL PRODUCTS - FINISHED ND RW MTERILS Microbiology Tests Detection: In house methods based on European Pharmacopoeia Clostridia spp Pseudomonas spp Salmonella spp ESM/M/700 E Staphylococcus aureus Enterobacteriaceae Escherichia coli Bacterial Endotoxin Enumeration: Total Viable Count (including fungi, yeasts and moulds) In-house method PHRM-M008 based on the el Clot ssay ESM/M/700 E E Preservative Efficacy Test M/M/380 E PURIFIED WTER and waters for injection (including endoscopy waters and washer disinfector rinse waters) Total erobic Colony Count M/M/362 in house method based on based on HTM 2030 (rchived) and CFPP Part D and CFPP E ssessment Manager: LH Page 25 of 34
26 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical PURIFIED WTERS (including endoscopy waters) and WTER FOR INJECTION Chemical Tests and Physical Tests cidity lkalinity luminium mmonium Calcium Calcium and Magnesium (hardness) Character/ppearance/ particulate matter (visual assessment) Carbon dioxide Chloride Nitrate Sulphate Documented In-House Method CHEM 123 based on current BP, EP, USP qualitative analysis and HTM 2030/2031 NHS guidelines where specified E Heavy metals - expressed as lead Documented In-House Method CHEM 123 based on current BP, EP and USP qualitative analysis E Oxidisable substances Documented In-House Method CHEM 123 based on current BP, EP and USP qualitative analysis E Quantitative nalysis: Organic Carbon: Total CHEM 125 based on current USP/BP/EP E Conductivity CHEM 128 based on current USP/BP/EP and HTM 2030/2031 NHS guidelines E WTER FOR INJECTION ph CHEM 022 based on current USP/BP/EP and HTM 2030/2031 NHS guidelines E Total Solids/Residue on Evaporation Documented In-House Methods CHEM 037 based on current BP/EP methods and HTM 2030/2031 NHS guidelines E ssessment Manager: LH Page 26 of 34
27 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical DISINFECTNTS, NTISEPTICS, BIOCIDES Microbiological Testing Documented in-house methods based on BS EN standard methods in full or with customer specified parameters Bacterial quantitative suspension test for food, industrial, domestic and institutional applications PHRM-M012 based on BS EN 1276:2009 E Fungal quantitative suspension test for food, industrial, domestic and institutional applications PHRM-M013 based on BS EN 1650: :2013 E Bacterial spore quantitative suspension test for food, industrial, domestic and institutional applications PHRM-M014 based on BS EN 13704:2002 E Quantitative surface test for food, industrial, domestic and institutional applications PHRM-M015 based on BS EN 13697:2001 E END Table 1: Pesticides determined by in-house method M/R/1003 QuEChERS extraction with LC-MS/MS and C-MS/MS Detection performed at location only LC-MS/MS cephate cetamiprid ldicarb-sulfone ldicarb-sulfoxide midosulfuron minocarb trazine zaconazole zamethiophos zoxystrobin C-MS/MS 1,4-Dimethylnaphthalene cetochlor cibenzolar-s-methyl crinathrin lachlor ldrin metryn nthraquinone traton trazine ssessment Manager: LH Page 27 of 34
28 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical LC-MS/MS Beflubutamid Benalaxyl Bendiocarb Benzoximate Bifenazate Bifenthrin Bixafen Boscalid Bromacil Bupirimate Buprofezin Butralin Carbaryl Carbendazim Carbetamide Carboxin Carfentrazone-ethyl Chlorantraniliprole Chlorfenvinphos Chloridazon Chlorothiophos Chlorotoluron Chloroxuron Chlorpyrifos Chlorpyrifos-methyl Chlorsulfuron Cinidon-ethyl Clomazone Coumaphos Crufomate Cyanazine Cycluron Cyflufenamid Cymiazol HCl Cymoxanil Cyproconazole Cyprodinil DEET Demeton-S-methyl-sulfone Desmedipham C-MS/MS zaconazole zinphos-methyl zoxystrobin Benalaxyl Benfluralin Bifenox Bifenthrin Biphenyl Bitertanol Bromocyclen Bromophos Bromophos-ethyl Bromopropylate Bromuconazole Bupirimate Buprofezin Butralin Cadusafos Carbofuran Carbophenothion Chinomethionat Chlordane, cis- Chlordane, trans- Chlorfenson Chlorfenvinphos Chlormefos Chlorobenzilate Chloroneb Chloropropylate Chlorpropham Chlorpyrifos Chlorpyrifos Methyl Chlorthal-dimethyl Chlorthion Chlozolinate Climbazole Cloquintocet-mexyl Crimidine Cyanofenphos Cyanophos ssessment Manager: LH Page 28 of 34
29 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical LC-MS/MS Desmethyl-pirimicarb Desmetryn Diazinon Dichlorvos Diclofop-methyl Dicrotophos Diethofencarb Difenoconazole Diflubenzuron Dimethachlor Dimethenamide Dimethoate Dimethomorph Dimethylvinphos Dimoxystrobin Diniconazole Dinotefuran Dioxacarb Diphenamid Disulfoton-sulfone Disulfoton-sulfoxide Emamectin Epoxiconazole Ethidimuron(Sulfadiazole) Ethiofencarb Ethiofencarb-Sulfone Ethiofencarb-sulfoxide Ethion Ethirimol Ethofumesate Ethoprophos Etofenprox Fenamidone Fenamiphos Fenamiphos-sulfone Fenamiphos-sulfoxide Fenbuconazole Fenchlorphos-oxon Fenhexamid Fenobucarb C-MS/MS Cyfluthrin Sum Cyhalothrin (lambda) Cypermethrin Cyproconazole Cyprodinil DDD, o,p'- DDD, p,p'- DDE, o,p'- DDE, p,p'- DDT, o,p'- DDT, p,p'- Deltamethrin Desmetryn Di-allate Diazinon Dichlobenil Dichlofenthion Dichlorvos Diclobutrazol Diclofop methyl Dicloran Dicofol Dicrotophos Dieldrin Dimethomorph Dioxabenzofos Dipropetryn Edifenphos Endosulfan (alpha isomer) Endosulfan (beta isomer) Endosulfan sulfate Endrin EPN EPTC Etaconazole Ethofumesate Ethoprophos Etoxazole Etridiazole Etrimfos ssessment Manager: LH Page 29 of 34
30 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical LC-MS/MS Fenoxaprop-P-ethyl Fenoxycarb Fenpropathrin Fenpropidin Fenpropimorph Fenpyroximate Fenthion Fenthion-sulfone Fenthion-sulfoxide Fenuron Flamprop-isopropyl Fluazinam Flufenacet Flufenoxuron Flumetsulam Flumorph Fluometuron Fluoxastrobin Flupyrsulfuron-methyl Flurtamone Flusilazole Flutolanil Formothion Fosthiazate Fuberidazole Furalaxyl Furathiocarb uthion Haloxyfop-methyl Hexaconazole Hexazinone Hexythiazox Imazalil Imidacloprid Iodosulfuron-methyl Ipconazole Iprobenfos Iprovalicarb Isoprothiolane C-MS/MS Famphur Fenarimol Fenchlorphos Fenchlorvos-oxon Fenitrothion Fenpiclonil Fenpropathrin Fenpropimorph Fenson Fenvalerate Flamprop-isopropyl Fluazifop-p-butyl Flucythrinate Fludioxonil Flurpirimidol Flusilazole Flutolanil Flutriafol Fluvalinate-tau Fonofos Furalaxyl Furathiocarb HCB HCH-alpha isomer HCH-beta isomer HCH-delta isomer Heptachlor Heptachlor endo-epoxide (trans) Heptachlor exo-epoxide (cis) Heptenophos Hexaconazole Imazalil Iodofenphos Iprodione Isazophos Isobenzan Isodrin Isofenphos Isoprocarb ssessment Manager: LH Page 30 of 34
31 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical LC-MS/MS Isoproturon Isopyrazam Isoxaben Kresoxim-methyl Lenacil Linuron Malaoxon Malathion Mandipropamid Mecarbam Mephosfolan Mepronil Metaflumizone Metalaxyl Metamitron Metazachlor Metconazole Methabenzthiazuron Methacrifos Methamidophos Methidathion Methiocarb Methiocarb-sulfone Methiocarb-sulfoxide Methomyl Methoprotryne Methoxyfenozide Metobromuron Metolachlor Metolcarb Metoxuron Metrafenone Metsulfuron-methyl Mevinphos Mexacarbate Monocrotophos Monolinuron Monuron Myclobutanil Napropamide C-MS/MS Isoxadifen-ethyl Isoxathion Kresoxim-methyl Leptophos Lindane (HCH-gamma) Malaoxon Malathion MCP thioethyl Mefenapyr-diethyl Mepanipyrim Metalaxyl Metazachlor Metconazole Methacrifos Methamidophos Methidathion Metribuzin Mevinphos Mirex Monocrotophos Myclobutanil Napropamide Nitrofen Nitrothal-isopropyl Nuarimol Ofurace Omethoate Oxadixyl Oxyfluorfen Paclobutrazol Parathion ethyl Parathion methyl Pebulate Penconazole Pendimethalin Pentachloroaniline Pentachloroanisole Pentachlorobenzene Pentachlorophenol Permethrin ssessment Manager: LH Page 31 of 34
32 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical LC-MS/MS Nitenpyram Norflurazon Omethoate Oxadiazon Oxadixyl Oxamyl Oxydemeton-methyl Paclobutrazol Paraoxon Paraoxon-methyl Penconazole Pencycuron Pendimethalin Penthiopyrad Permethrin Pethoxamid Phenmedipham Phenthoate Phorate-sulfone Phorate-sulfoxide Phosalone Phosfolan Phosmet Phosphamidon Phoxim Picolinafen Picoxystrobin Pinoxaden Piperonyl-butoxide Pirimicarb Pirimiphos-ethyl Pirimiphos-methyl Prochloraz Procymidone Profenofos Promecarb Prometon Prometryne Propamocarb Propanil C-MS/MS Perthan Phenothrin Phenthoate Phosfolan Phosmet Phosphamidon I Phosphamidon II Piperonyl butoxide Pirimicarb Pirimiphos-ethyl Pirimiphos-methyl Procymidone Profenofos Prometryn Propachlor Propargite Propazine Propetamphos Propham Propiconazole Propyzamide Prothiofos Pyrazophos Pyrimethanil Quintozene Resmethrin Silafluofen Simazine Sulfotep Sulprofos Tebuconazole Tecnazene Tefluthrin, cis- Terbacil Terbufos Terbumeton Terbuthylazine Terbutryne Tetrachlorvinphos Tetradifon ssessment Manager: LH Page 32 of 34
33 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical LC-MS/MS Propaquizafop Propargite Propazine Propham Propiconazole Propoxur Propyzamide Proquinazid Prosulfocarb Prothioconazole-desthio Pyracarbolid Pyraclostrobin Pyraflufen-ethyl Pyridaben Pyrifenox Pyrimethanil Pyriproxyfen Quassin Quinoxyfen Quizalofop-ethyl Rimsulfuron Rotenone Silthiofam Spinetoram-major Spinetoram-minor Spinosyn- Spinosyn-D Spirodiclofen Spirotetramat Spiroxamine Tebuconazole Tebufenozide Tebufenpyrad Tebupirimfos Tebuthiuron Temephos Terbufos-sulfone Terbufos-sulfoxide Terbuthylazine Terbutryn C-MS/MS Tetramethrin Tetrasul Thiabendazole Tolclofos-methyl Transfluthrin Triadimefon Triadimenol Tri-allate Triazamate Trichloronate Trietazine Trifloxystrobin Trifluralin Zoxamide ssessment Manager: LH Page 33 of 34
34 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LS Food and Pharmaceutical LC-MS/MS Tetraconazole Tetramethrin Thiabendazole Thiacloprid Thiamethoxam Thidiazuron Thifensulfuron-methyl Thiobencarb Thiodicarb Tralkoxydim Triadimefon Triadimenol Triazophos Trichlorfon Tricylazole Tridemorph Trietazine Trifloxystrobin Triflumizole Triflusulfuron-methyl Trimethacarb Uniconazole-P Vamidothion XMC Zoxamide C-MS/MS END ssessment Manager: LH Page 34 of 34
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