Science Academies Refresher Course on Modern Biotechnology: Concepts & Practice (MBCP 2013) Sponsored by

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1 Science Academies Refresher Course on Modern Biotechnology: Concepts & Practice (MBCP 2013) May 15 th & 29 th 2013 Organized by School of Biotechnology Madurai Kamaraj University, Madurai Tamil Nadu, INDIA Sponsored by Indian Academy of Sciences Indian National Science Academy National Academy of Sciences Bangalore New Delhi India, Allahabad Submitted By Dr. G. Marimuthu Course Director, MBCP-2013 School of Biological Sciences Madurai Kamaraj University Madurai Dr. B. Ashokkumar Course Co-ordinator, MBCP-2013 School of Biotechnology Madurai Kamaraj University Madurai

2 MODERN BIOTECHNOLOGY - CONCEPTS AND PRACTICE Science Academies Refresher Course on Modern Biotechnology - Concepts and Practice sponsored by Indian Academy of Sciences, Bangalore, Indian National Science Academy, New Delhi and National Academy of Sciences, Allahabad was Organized at School of Biotechnology, Madurai Kamaraj University, Madurai for 14 days from May 15 th May 29 th This course was designed to train the faculty and researchers on modern biotechnology and the aim of the Refresher s course was to expose the participants to get both theoretical and practical knowledge on concepts and techniques in biotechnology and to widen their knowledge in recent biotechnological concepts. The program commenced with registration and refreshment at 9.30 am on May 15, Wednesday. Twenty two registered teaching faculty and researchers from the educational institutes all over India, were participated. This course was inaugurated officially by the honorable Vice-Chancellor of Madurai Kamaraj University, Dr. Kalyani Mathivanan. The welcome Address was given by Dr. K. Veluthambi, the Head and Chairperson, School of Biotechnology. The presidential address was delivered by Dr. Kalyani Mathivanan, the Vice- Chancellor, MKU followed by the Inaugural address by Dr. G. Marimuthu, the Course Director, MBCP-2013 and the Keynote address was delivered by Dr. P. Palanivelu, Professor and Head, Department of Molecular Microbiology, School of Biotechnology. The inaugural function was ended with a vote of thanks given by Dr. B. Ashokkumar, the Course Co-ordinator. The actual course was started from May15 th afternoon with the lectures given by various eminent scientists in the forenoon and hands on training in the afternoon. Every day, we had guest lectures on diverse topics in Biotechnology in the forenoon sessions. Eminent Scientist Dr. K. Thangaraj, CCMB, Hyderabad, had delivered an excellent lecture on Genetic patterns in Indian populations and Allele frequency differences between ethnic groups in India and Europe. Also he focused how the Indian genetics is different from rest of the world and what would be its implications in health and disease. Genetic testing (also called DNA-based tests) is among the newest and most sophisticated of techniques used to test for genetic disorders which involves direct examination of the DNA molecule itself and how these genetic tests are very useful for human was also taught by Prof. C. Adithan, JIPMER, Pondicherry.

3 Dr. Jayandharan, Christian Medical College, Vellore, had delivered a lecture about Gene therapy poses one of the greatest technical challenges in modern medicine and how it is very hard to introduce new genes into cells of the body. Dr. P. Sundaresan, Aravind Medical Research Foundation, Madurai; Dr. G. Kumaresan, School of Biological Sciences, MKU; Dr. S. Sudhakar, Manonmaniam Sundaranar University, Tirunelveli and Prof. K. Sundar, Kalasalingam University, were elaborated the valuable current scenario of Medical Biotechnology. Energy crisis and the climate change caused by use of petrochemicals that increases emission of carbon dioxide leads global warming are the today s major problem in our earth. So, in order to address these problems and find the solution for this we invited Dr. R. Rengasamy, University of Madras and Dr. N. Thajuddin, Bharathidasn University, Tiruchirappalli as the experts in from the field of biofuels. The invitees expressed their dedication in the field of biofuel production from microalgae. Dr. K. Chandra Raj, IIT Madras also delivered detailed information about bioethanol production strategies from various microbial sources. To meet the increasing demand for food, we have been resorting to intensive cultivation practices with the use of chemical fertilizers. The extensive use of chemical fertilizers leads to cause deterioration of the fertility of top soil. So what are possible ways to improve the crop yield by using cyanobacteria that plays an important role in the plant responses to growth and development was elaborated by Prof. M. Sundararaman and Prof. P. Malliga, NFMC, Bharathidasan University, Tiruchirappalli. Strain development and metabolic pathway engineering for the production of 3- hydroxypropionic acid from glycerol/glucose at significant scales was dealt by Dr. S. Mohanraj, PRIST University, Thanjavur. Furthermore, the common problems in recombinant protein production and strategies for better solutions were discussed by Dr. S.R. Senthil kumar, Head, R&D, Sandwoods Pvt Ltd, New Delhi. Cloning, characterization and overexpression of a thermophilic fungal chitinase in S. cerevisiae for chitinase production was clearly taught by Prof. P. Palanivelu, Head, Department of Molecular Microbiology MKU. Gene silencing is being widely used in modern science as a method to study the function of genes. In order to intricate

4 the detailed information to participants Prof. K. Veluthambi, delivered a lecture on Lessons learnt on gene silencing from transgenic plants with inverted T-DNA repeats. We invited the people from Indian Council of Medical Research (ICMR) Dr. R. Paramasivam and Mr. M. Muniaraj to create awareness about the mosquito borne diseases. Every day afternoon sessions, the participants were taught with various modern techniques like DNA isolation from various sources including plants, Plasmid and RNA Isolation techniques, 16S rdna Analysis, C. elegans - handling and maintenance, RT-PCR, Quantitative PCR. The participants were taken for a one-day trip to Kodaikanal for their relaxation from normal schedule and it was an exciting trip for them on seeing the natural beauty of Kodaikanal and they really enjoyed the cool weather. After arrival, the routine sessions were there with the lecture and practical sessions. On the verge of course completion, feedbacks were obtained from the participants. Finally, the 14 days refresher s course ended on March 29 th, 2013 with the valedictory function. Dr. Anitha Sironmani, Head, Department of Genetic Engineering, School of Biotechnology, welcomed all the guests. Dr. K. Pitchumani, Registrar, MKU was the chief guest, who presided over the valedictory function and felicitated both the organizers and participants of the program. The refresher course was finally concluded with conclusive report presented by Dr. P. Varalakshmi, Assistant Course co-ordinator and vote of thanks was given by Dr. N. Sivakumar, Assistant Course co-ordinator. Participants were provided course completion certificate as per the directions of Science Academy.

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7 SCIENTIFIC PROGRAMME Science Academies Refresher Course on Modern Biotechnology: Concepts and Practice AM Registration AM Inauguration AM Tea break PM Prof. M. Krishnan Bharathidasan University, Tiruchirappalli. Title: Characterization of lipophorin in lepidopteran insect Silkworm, Bombyx mori and its role in lipid transport to developing oocytes PM Lunch AM Dr. S. Mohanraj PRIST University, Thanjavur. Title: Development of recombinant strains and metabolic engineering for the production of 3- hydroxypropionic acid PM Dr. K. Thangaraj CCMB, Hyderabad. Title: Population stratification: Does it have any impact on health and disease? PM Lunch Genomic DNA Isolation from Blood Genomic DNA Isolation from Cyanobacteria AM Prof. Hussain Munavar Madurai Kamaraj University, Madurai PM Prof. C. Adithan JIPMER, Pondicherry. Title: Pharmacogenomics and personalized medicine.

8 PM Lunch Genomic DNA Isolation from Bacteria Genomic DNA Isolation from Plant AM Dr. GR. Jayandharan Christian Medical College, Vellore Title: AAV Mediated Gene Therapy PM Prof. N. Thajuddin Bharathidasan University, Tiruchirappalli. Title: Microalgae: Biodiversity to Biotechnology PM Lunch 16S rrna PCR analysis AM Dr. P. Sundaresan Aravind Medical Research Foundation, Madurai Title: Molecular genetics of inherited eye diseases and gene therapy PM Prof. M. Sundararaman Bharathidasan University, Tiruchirappalli Title: Potent toxins of marine cyanobacteria and its pharmaceutical potentials PM Lunch Bacterial Transformation AM Prof. R. Rengasamy University of Madras, Chennai. Title: Biofuel production from microalgae PM Dr. C. Sankaranarayanan Saraswathi Narayanan College, Madurai. Title: Biotechnological applications of cyanobacteria.

9 PM Lunch Transformation screening and selection AM Prof. P. Malliga Bharathidasan University, Tiruchirappalli. Title: Cyanobacterial defacement? An over view PM Prof. K. Veluthambi Madurai Kamaraj University, Madurai. Title: Lessons learnt on gene silencing from transgenic plants with inverted T-DNA repeats PM Lunch Plasmid Isolation, Restriction Digestion, Agarose Gel Electrophoresis AM Dr. A. Palavesam CMST, Manonamaniam Sundaranar University, Tirunelveli. Title: Probiotics and Prebiotics PM Dr. G. Kumaresan Madurai Kamaraj University, Madurai. Title: Genomics for Diagnostics and Targeted Therapeutics PM Lunch C. elegans Handling, Media Preparation AM Dr. R. Paramasivan Centre for Research in Medical Entomology, ICMR, Madurai. Title: Molecular diagnosis of vector borne diseases: special emphasis on dengue PM Prof. G. Marimuthu Madurai Kamaraj University, Madurai. Title: Clock gene polymorphisms and people with psychiatric disorders

10 PM Lunch ICMR Molecular diagnosis of dengue by PCR AM Dr. S. Sudhakar Manonmaniam Sundaranar University Tirunelveli. Title: Autofluorescence of Stem Cell and Function of Riboflavin in Regeneration PM Prof. K. Sundar Kalasalingam University, Krishnan Koil. Title: Helminth infection modulates autoimmunity in mice PM Lunch RNA Isolation, cdna synthesis AM Mr. M. Muniaraj Centre for Research in Medical Entomology Field Station, Vridhachalam Title: Microbial Association with Insects: an unexplored potential area for novel enzymes and bioactive-compounds PM Dr. S. R. Senthil Kumar Sandwoods Lifesciences (P) Ltd, New Delhi. Title: Production of Recombinant Proteins in High Density Cultures - Challenges and Solutions PM Lunch - RT- PCR AM Dr. K. Chandra Raj IIT Madras, Chennai PM Dr. K. Balamurugan Alagappa University, Karaikudi. Title: Caenorhabditis elegans as a model to study molecular changes during Host- Pathogen interactions

11 PM Lunch Discussion & Feedback AM Prof. P. Palanivelu Madurai Kamaraj University, Madurai Title: Cloning, characterization and over expression of a thermophilic fungal chitinase in S. cerevisiae PM Dr. M. Saravanan IIT, Kanpur. Title: Engineering sequence specificity of Nucleases PM Lunch PM Valedictory Function

12 List of Resource Persons Science Academies Refresher Course on Modern Biotechnology: Concepts and Practice 1. Dr. M. Krishnan Professor & Head Department of Environmental Biotechnology Bharathidasan University Tiruchirappalli , Tamil Nadu. profmkrish@gmail.com 2. Dr. K. Thangaraj Senior Principal Scientist Centre for Cellular and Molecular Biology Hyderabad, India E.mail: thangs@ccmb.res.in 3. Dr. S. Mohanraj Centre for Research and Development PRIST University Thanjavur, Tamil Nadu. E.mail: rajmohanhp@gmail.com 4. Dr.C. Adithan Senior Professor & Head, Department of Pharmacology JIPMER, Pondicherry E.mail: adithan@yahoo.com 5. Dr. Jayandharan GR Department of Hematology and Centre for Stem Cell Research Christian Medical College Vellore , Tamil Nadu. E.mail: jay@cmcvellore.ac.in 6. Dr. N. Thajuddin Professor & Head Department of Microbiology School of Life Sciences Bharathidasan University Tiruchirappalli , Tamil Nadu.

13 E.mail. 7. Dr. P. Sundaresan Senior Scientist Department of Genetics Aravind Medical Research Foundation, Madurai , Tamil Nadu E.mail. 8. Dr. M. Sundararaman Professor, Department of Marine Biotechnology School of Marine Sciences Bharathidasan University Tiruchirappalli , Tamil Nadu. E.mail: sundarbdu1@gmail.com 9. Dr. R. Rengasamy Professor, CAS in Botany University of Madras Chennai , Tamil Nadu. E.mail: profrrengasamy@yahoo.com 10. Dr. C. Sankaranarayanan Professor Department of Botany Saraswathi Narayanan College Madurai, Tamil Nadu. E.mail: csankaramarayanan@gmail.com 11. Dr. P. Malliga Professor Department of Marine Biotechnology National Facility for Marine Cyanobacteria Bharathidasan University Tiruchirappalli , Tamil Nadu. E.mail: malli62@yahoo.com 12. Dr. K. Veluthambi Professor & Head Department of Plant Biotechnology School of Biotechnology Madurai Kamaraj University Madurai , Tamil Nadu. E.mail: kveluthambi@rediffmail.com

14 13. Dr. A. Palavesam Professor and Head Centre for Marine Science and Technology Manonmaniam Sundaranar University Rajakkamangalam Tamilnadu, India Dr. G. Kumaresan Associate Professor & Head Department of Genetics School of Biological Sciences Madurai Kamaraj University Madurai , Tamil Nadu. kumar@oncocellomics.org 15. Dr. R. Paramasivan Scientist 'D' Centre for Research in. Medical Entomology (ICMR) 4, Sarojini Street Madurai , Tamil Nadu rpsivan2000@yahoo.co.in 16. Dr. G. Marimuthu Professor & Head Department of Animal Behaviour & Physiology School of Biological Sciences Madurai Kamaraj University Madurai , Tamil Nadu. E.mail: boxgm@gmil.com 17. Dr. S. Sudhakar Head, Department of Biotechnology Manonmaniam Sundaranar University Alwarkurichi , Tirunelveli Dt. Tamil Nadu. E.mail: sudhakarmsu@yahoo.com 18. Prof. K. Sundar Associate Professor Department of Biotechnology Kalasalingam University Krishnan Koil, Tamil Nadu. E.mail: sundarkr@yahoo.com

15 19. Mr. M. Muniaraj Scientist C/ In-Charge Centre for Research in Medical Entomology Field Station (Indian Council of Medical Research) Vridhachalam, Tamil Nadu. E.mail: 20. Dr. S. R. Senthilkumar Head Research & Development Sandwoods Life Sciences Private Limited Okhla Industrial Area, Phase II New Delhi E.mail. senthikumarsr@sandwoods.com 21. Dr. K. Chandra Raj Associate Professor Department of Biotechnology IIT Madras Chennai, Tamil Nadu. E.mail: kcraj@iitm.ac.in 22. Dr. K. Balamurugan Associate Professor Department of Biotechnology Alagappa University Karaikudi , Tamil Nadu. E.mail: bsuryar@yahoo.com 23. Dr. P. Palanivelu Professor and Head Department of Molecular Microbiology School of Biotechnology Madurai Kamaraj University Madurai , Tamil Nadu. E.mail: ppmkupp@gmail.com 24. Dr. Saravanan Matheshwaran Department of Biological Sciences and Bioengineering Indian Institute of Technology Kanpur (U.P). E.mail: saran@iitk.ac.in

16 List of Participants Science Academies Refresher Course on Modern Biotechnology: Concepts and Practice S. No NAME & ADDRESS 1. Mr. Sanjay Navnitlal Parekh B 302, Shashank Park Near Gujarat Gas Company Adajan, Surat , Gujarat. sparekh97@gmail.com 2. Dr. J. Madhukar Department of Biochemistry Kakatiya University Warangal A.P madhuj.biochem@gmail.com 3. Dr. Melwyn D Cunha SJ St Aloysius College/ Residence Department of Biotechnology P.B.720, Kodialbail po, Light House Hill Road Mangalore Karnataka- India. meiwynsj@gmail.com 4. Dr. Y. Venkaiah of Zoology Kakatiya University,Vidyaranyapuri Hanamkonda Warangal, A.P. India. venkaiahyanamala07@gmail.com 5. Mr. Ambarish S. Sindagi Lecturer, Karnatak Science College Department of Microbiology

17 Dharwad Dr. Ahmed Abdul Haleem Khan SRNK Govt. Degree College Desaipet, Banswada Nizamabad, A.P Dr. R. Krishnamoorthy Lecturer Department of Biotechnology Bharathidasan University, Trichirapalli Dr. Dharmesh Harwani Department of Microbiology Maharaja Ganja Singh University, Bikaner Dr. T. Francis Xavier Department of Botany St. Joseph s College, Trichirappalli. E.mial: mycofrancis@yahoo.co.in 10. Dr. R. Thirugnanasampandan PG and Research Department of Biotechnology Kongunadu Arts and Science College GN mills, Coimbatore , Tamil Nadu. rtsampandan@yahoo.com 11. Mr. K. Abdhul, in Biotechnology, Nandha Arts and Science College, Erode.

18 Dr. M. Ayyanar Lecturer Department of Botany Pachaiyappa s College, Chennai asmayyanar@yahoo.com 13. Dr. M. Selvi in Botany Sri Parasakthi College for women Courtallam E.mail: selvirajeswari_1972@yahoo.in 14. Dr. R. Ramasubbu Department of Biology The Gandhigram Rural Institute, Dindigul racprabha@gmail.com racprabha@yahoo.com 15. Dr. P.S. Arunadevi PG and Research, Department of Zoology Sri Vasavi College, Erode ; aruna_enscience@rediffmail.com 16. Mr. S. Miller Samson PG and Research Department of Biotechnology Nehru Memorial College Puthanampatti, Trichy. millermdu@gmail.com 17. Dr. M. Melvin Joe Plot No. 228, NH Colony Perumalpuram Tirunelveli. micromelvin@gmail.com

19 18. Ms. R. Sridurgdevi Department of Biotechnology Sri Krishna Arts and Science College Coimbatore Ms. J. Christina Rosy Department of Biotechnology Kalasalingam University Krishnan Koil Mr. Sri Ramkumar Sivanthi Aditanar College Pilayarpuram Dr. M. Baskaran PG and Research Department of Zoology and Wild Life Biology A.V.C College, Mannampandal Mayiladuthurai beebaskaran@rediffmail.com 22. Ms. S. Shanmugapriya Lecturer, Department of Microbiology and Biotechnology SVN College, Nagamalai Madurai Priyasg87@gmail.com