ncounter FOR TRANSLATIONAL RESEARCH.

ncounter FOR TRANSLATIONAL RESEARCH.

HIGHLY MULTIPLEXED SINGLE MOLECULE COUNTING FASTER THAN qpcr. SIMPLER THAN NGS. NanoString Technologies provides tools for researchers solving problems in the areas of translational research and molecular diagnostics. The ncounter platform is a complete solution for digitally detecting and counting large sets of molecules. Digital molecular barcoding technology enables researchers to count the number of unique transcripts in each sample. These barcodes have significant multiplexing power so you can measure up to 800 RNA, DNA, or protein targets in every sample. Digitally examine multiple pathways in a single tube. Spend less time on sample prep and data analysis. Work with difficult sample types, including FFPE tissue. Examine multiple analytes simultaneously with true multi-omic capabilities. 3

LESS TIME + BETTER DATA Don t just take our word for it. In a recent survey*, NanoString customers told us how the ncounter platform compared to their previous method: 78% SPEED 89% PRECISION 98% EASE-OF-USE Said They Cut Their Project Time by Half or Greater. Said They Detect >.67-Fold Change Sensitivity. Said ncounter Is Easier to Use Than Previous Methods. * Source: 204 TechValidate survey of NanoString Technologies customers. TechValidate is a trusted third-party research organization that directly interfaces with business and technology end users to collect and validate information about their products' deployments. More information is available at www.techvalidate.com. 4

BECAUSE DISCOVERY CAN'T WAIT SIMPLE WORKFLOW Each color-coded barcode represents a unique target molecule. Barcodes hybridize directly to target molecules and can be individually counted without the need for amplification*, providing very sensitive digital data. * Single cell assays require amplification prior to sample processing and data collection. Solution Phase Hybridization HYBRIDIZE Capture Probe Reporter Probe Target-Probe Complex 2 Two probes hybridize directly to a target molecule in solution. The Reporter Probe carries the fluorescent barcode and the Capture Probe contains a biotin moiety that immobilizes the hybridized complex for data collection. PURIFY + IMMOBILIZE Target Excess Probes Removed Hybridized Probes Bind to Cartridge Complexes Are Immobilized and Aligned on Cartridge HIGHLY AUTOMATED TECHNOLOGY WITH NO LIBRARY PREP After hybridization, samples are transferred to an ncounter instrument, which removes excess probes. Purified target-probe complexes are bound, immobilized, and aligned on the imaging surface of the ncounter Cartridge. 3 COUNT Barcode Counts Identity Sample cartridges are scanned by an automated fluorescence microscope. Barcodes are counted for each target molecule, and the data are exported as a simple CSV file. 3 XLSA 2 FOX5 INSULIN

ncounter SPRINT ncounter MAX FOR RESEARCH LABS An affordable benchtop device that can analyze differential expression of genes and proteins. FOR CORE LABS Expandable throughput to analyze differential expression of genes and proteins. MAXIMIZE SAMPLE Don t let samples limit your experimental design. Analyze s to 0s of targets simultaneously using single-tube multiplexing, and avoid waste by eliminating RNA extraction; process crude cell lysates from as few as 2,500 cells. Hands-on Time Prepare hybridized samples: 5 minutes OPTIONS FOR GROWTH NanoString provides the option to grow into the MAX configuration. Start with one Prep Station and one Digital Analyzer. Add a second Prep Station when needed and double your capacity to match a growing number of users. Hands-on Time Prepare hybridized samples: 5 minutes BOOST PRODUCTIVITY Enjoy an intuitive workflow that requires only minutes of hands-on time from sample to data. Deploy this technology without the need to hire additional staff, and free your team to advance other parts of your project. SIMPLIFY DATA Don t waste time waiting for a bioinformatics specialist to review your data. Get results in a simple CSV file that contains direct counts for each target. Import it into your favorite application or use the included nsolver Analysis Software for convenient data analysis. DETECT SMALL CHANGES Eliminates cdna synthesis, amplification*, and library prep so you experience less technical variation in your assay. See real differences in your sample and work faster by reducing the need for experimental replicates. * Single cell assays require amplification prior to sample processing and data collection. Initiate run on SPRINT Profiler: 5 minutes Run Time 6 hours, minutes Runs per Day 2 runs ncounter Cartridge Format Microfluidic process can purify hybridized samples and collect data in one device. MAXIMIZE THROUGHPUT Two Prep Stations and a Digital Analyzer help eliminate bottlenecks in sample processing and data collection. Process 96 lanes per day and up to 384 samples with additional sample plexing. Automated liquid-handling robots minimize hands-on time. SECURE ACCESS An enterprise software package is included for laboratories that require enhanced security. Control user access, automate data flow, and generate audit logs. UNATTENDED DATA COLLECTION The Digital Analyzer can load up to six cartridges at a time for unattended scanning overnight. Random access enables users to load or remove additional cartridges without waiting for the current run to finish. Initiate run on Prep Station: 5 minutes Transfer to Digital Analyzer: 5 minutes Run Time Prep Station: 2.5 to 3 hours Digital Analyzer: 0.5 to 5 hours Runs per Day Prep Station: 4 runs Digital Analyzer: capacity for 6 cartridges ncounter Cartridge Format Prep Station purifies hybridized samples and deposits them onto a glass slide which is transferred to the Digital Analyzer.

ncounter FLEX FOR CLINICAL APPLICATIONS Flexible platform used to translate oncology signatures from research to the clinic. CLINIC-READY PLATFORM The Prosigna Breast Cancer Prognostic Gene Signature Assay has received 5(k) clearance for use with the ncounter Dx Analysis System with FLEX configuration. ncounter FLEX is manufactured under GMP guidelines and ISO 3485 to ensure quality and compliance with international standards. TRANSLATIONAL SUITE ncouner FLEX can operate in two distinct modes: Diagnostics and Life Sciences. In addition, an enterprise software package is included to control user access, automate data flow, and generate audit logs. TUMOR ANALYSIS Analyze gene expression without a cdna synthesis step, enabling greater tolerance of RNA degradation and inhibitors associated with the formalin fixation process. Improve confidence when analyzing FFPE tissue such as tumors that can be matched to known patient outcomes. DEVELOP TAILORED SIGNATURES Reduce large gene sets (up to 800 targets) with a single ncounter CodeSet and single-tube multiplexing technology. Quickly reach a focused gene signature that will robustly identify tumor subtypes without the challenge of optimizing and maintaining components needed to support multiple RT-qPCR assays. Hands-on Time Prepare hybridized samples: 5 minutes Initiate run on Prep Station: 5 minutes Transfer to Digital Analyzer: 5 minutes Run Time Prep Station: 2.5 to 3 hours Digital Analyzer: 0.5 to 5 hours Runs per Day Prep Station: 4 runs Digital Analyzer: capacity for 6 cartridges ncounter Cartridge Format Prep Station purifies hybridized samples and deposits them onto a glass slide, which is transferred to the Digital Analyzer.

EXPLORE THE POTENTIAL OF YOUR RESEARCH AN INSTRUMENT FOR EVERY NEED Gene Expression Analyze up to 800 genes simultaneously in purified RNA, cell lysates, or FFPE tissue. ncounter Products ncounter SPRINT ncounter MAX ncounter FLEX Prosigna No No Yes Enterprise Package No Yes Yes Expandable with Additional Prep Station No Yes Yes ncounter Elements Yes Yes Yes ncounter Life Science Assays Yes Yes Yes Throughput (Lanes per Day) 24 48-96* 48-96* * Additional Prep Station required for > 48 lanes per day. TENS OF THOUSANDS OF DATA POINTS EVERY DAY Sample Multiplex # of Genes per Run Samples per Day Data Points per Day ncounter SPRINT ( sample per lane) 800 genes 24 samples = 9,200 ncounter FLEX ( sample per lane) 800 genes 48 samples = 38,400 ncounter MAX (2 Prep Stations; sample per lane) 800 genes 96 samples = 76,800 ncounter MAX (2 Prep Stations; 4 samples per lane) 200 genes 384 samples = 76,800 Copy Number Variation Quantify multi-allelic CNVs with minimal hands-on time. 8

Single Cell Analysis RNA:Protein Profiling Obtain single cell sensitivity with reverse transcription and limited amplification. Measure RNA and protein simultaneously for true multi-omic data. mirna Expression mirge Expression Profile mirbase high confidence and published clinical mirnas. Profile mirna and mrna expression simultaneously with one CodeSet. ChIP-String Assays Fusion Gene Analysis Skip the hassle of library prep and amplification when analyzing ChIP'd material. Find breakpoint locations and specific junction sequences. 9

PANELS TO HELP YOUR RESEARCH UNDERSTAND CANCER BIOLOGY WITH ncounter PANCANCER PANELS All cancers must evolve a means of sustaining self-sufficient growth and evading apoptosis. Each PanCancer panel contains 770 genes selected for their role in key aspects of cancer biology. PanCancer Pathways Panel Understand cancer biology and pathway deregulation. DNA Damage Control Transcriptional Regulation Wnt Hedgehog Angiogenesis PanCancer Immune Profiling Panel Explore the human or mouse immune response to cancer. Chromatin Modification TGF-β Apoptosis/ Cell Cycle RAS Cell Type Epithelial to Mesenchymal Transition Extracellular Matrix PanCancer Progression Panel Investigate cancer Notch PI3K Metastasis progression and metastasis. MAPK STAT COLLECT TRUE MULTI-OMIC DATA WITH RNA:PROTEIN PROFILING NanoString offers the only solution for simultaneous measurements of RNA and protein in a single assay. ncounter RNA:Protein Profiling technology uses DNA oligonucleotides that are bound to specific antibodies. With minimal sample preparation, these oligos can then be combined with purified total RNA from the same sample in a single hybridization reaction with ncounter probes. The PanCancer RNA:Protein Immune Profiling Assay is our first product to use this technology, combining 770 mrnas and 30 proteins to provide a comprehensive view of the cancer immune response. Counts of RNA and protein targets are easily compared because they are analyzed in the same assay, using the same instrument. UPDATED mirna EXPRESSION PANELS FOR HUMAN, RAT, AND MOUSE Enjoy highly specific and sensitive mirna profiling with NanoString s collection of mirna panels. Human v3 mirna Panel 800 human mirnas from mirbase v2 Rat v.5 mirna Panel 423 rat mirnas from mirbase v7 Mouse v.5 mirna Panel Over 600 murine and murine-associated viral mirnas from mirbase v5 All kits include a sample preparation kit with reagents for ligating unique oligonucleotides onto the mirnas of interest. An enzymatic purification step then removes any unligated material. Controls in every kit may be used to monitor ligation efficiency and specificity.

FLEXIBLE SAMPLE INPUTS INSIGHT FROM DIFFICULT SAMPLES ncounter assays can accept samples such as purified total RNA, raw cell or blood lysates, and formalin-fixed paraffin-embedded (FFPE) extracts with no loss in precision. Even severely degraded RNA can be a viable sample input. Many ncounter assays require only 0 ng or less of input material, which is ideal for investigators working with precious samples. This amount is equivalent to a single curl of FFPE tissue, and data are comparable to that generated with matched fresh-frozen material.,000,000 cells CRUDE CELL LYSATES Lysate Counts,000 0 5,000 cells 2,500 cells Three cell lysates (2,500, 5,000, and,000 cells) were used during sample hybridization and compared to 0 ng of purified total RNA. Results using cell lysates were highly correlated with purified RNA (R 2 > 0.97 for all three) and demonstrated that comparable data can be 0 Total RNA Counts,000,000 achieved with either protocol.,000 Lysate WHOLE BLOOD LYSATES,000 Lysate 2 Two PAXgene -lysed whole blood replicates were used during sample Lysate Counts 0 hybridization and compared to 0 ng of matched purified total RNA. Results using blood lysates were highly correlated with purified RNA (R 2 > 0.96 and R 2 > 0.97) and demonstrated that high quality data can be obtained 0 Total RNA Counts,000,000 using PAXgene-lysed whole blood. (PAXgene is a trademark of QIAGEN.),000 FORMALIN-FIXED PARAFFIN-EMBEDDED TISSUE Lysate Counts,000 0 FFPE-derived and purified total RNA were used during sample hybridization and compared to matched purified total RNA from fresh tissue. Results using FFPE-derived tissue were highly correlated with purified RNA (R 2 > 0.97) and demonstrated that high quality data can be 0 Total RNA Counts,000,000 achieved from FFPE.

DIGITAL PRECISION OBSERVE THE SMALLEST CHANGES Direct, digital counting technology reduces the risk of signal saturation, providing reliable data across a wide dynamic range. Correlation between technical replicates often exceeds 0.99. Lot-to-lot and site-to-site variability is minimal, facilitating long-term studies across multiple independent testing sites.,000 FEWER TECHNICAL REPLICATES Replicate 2 Counts,000 0 Gene counts from two technical replicates plotted against each other demonstrate the assay reproducibility over a wide dynamic range (-50,000 counts). One total RNA sample was split into two separate hybridization reactions and processed independently. These data demonstrated the high level of sensitivity and precision of the assay (R 2 = 0.999), even at very 0 Replicate Counts,000,000 low levels of expression. 35% PRECISION ACROSS A LARGE RANGE OF EXPRESSION Average Percent CV 25% 5% 5% 0 > 30 30 3 3 0.3 < 0.3 Genes were grouped by level of expression in 0 ng of total RNA and percent CV was calculated for each group. Many genes were expressed at less than one transcript per cell and could be measured with less than 5% CV, allowing quantitative measurements of 2-fold changes or less. Precision increase with level of expression, in some cases allowing for quantification of less than.2-fold. Transcript Copies per Cell Total RNA Counts (Fold Change),000,000 0.5x Down (y = 0.676x - 0.55).5x Up (y =.500x -.2) 2x Up (y=.965x - 0.467) 0,000 Total RNA Counts (0 ng),000 FRACTIONAL FOLD CHANGE MEASUREMENTS Variable total RNA inputs of the same sample demonstrate ncounter's ability to detect fractional fold change measurements. Total RNA input amounts of 67 ng, 50 ng, and 200 ng were compared against a baseline of 0 ng. Slopes correlated closely with the expected values of 0.67,.5, and 2 across all levels of expression. 2

Subtype Binding.Density Scanned.Date POWERFUL DATA ANALYSIS VISUALIZE RESULTS WITH nsolver ANALYSIS SOFTWARE nsolver Analysis Software is a free data analysis program with the ability to quickly and easily QC, normalize, and analyze ncounter data without the need to purchase additional software packages. To learn more about nsolver, visit www.nanostring.com/products/nsolver. Significance testing and error model for ratios. Include user-defined gene annotations. Perform cross-codeset analyses for long-term studies. Customized quality control for samples/lanes. Design, save, and edit user-defined experiments. Customized normalization and fold change measurements. Integrates with pathway applications. Compatible with Windows and Macintosh operating systems. DAN THBS Decorin Chordin BMP Growth factor LTBP TGFβ Noggin IFNγ TNFα TGF-BETA SIGNALING PATHWAY BAMBI BMPRI BMPRII BAMBI TGFβ RI TGFβ RII +p Ras/MAPK +p Smurf/2 +u TAK, MEKK, DAXX/JNK Smad/5/8 Smad6/7 Smad2/3 SARA Rbx Cul Skp +u MAPK signaling pathway Smad/5/8 Smad4 Smad2/3 Smad4 ERK +p +p Ubiquitin-mediated proteolysis DNA Id Transcription factors, co-activators, and co-repressors p7 E2F4/5 DP p300 SP DNA DNA DNA c-myc p5 Osteoblast differentiation, neurogenesis, ventral mesoderm specification Angiogenesis, extracellular matrix neogenesis, immunosuppression, apoptosis induction Apoptosis G arrest Cell Cycle SIMPLE, ADVANCED DATA ANALYSIS WITH NEW ANALYSIS MODULES With the upgrade to v2.5, nsolver Analysis Software now enables new, advanced analysis modules. These modules can be designed for specific CodeSets or panels SubtypeD to perform a wide range of automated data analysis and visualization tasks without sacrificing customization. Analysis modules bring together powerful academic and open-source analysis tools, provide a simple interface to guide you through the analysis, and display the results in an interactive HTML document. The PanCancer Pathways Advanced Analysis Module was the first customization introduced by NanoString and helps you perform statistically principled analyses of your ncounter PanCancer Pathways Panel data. Scanned.Date Binding.Density Subtype OR3443 OR3457 OR3458 OR3452 OR24 OR298 OR4 OR323 OR3242 OR3567 OR3005 OR304 OR2969 OR2968 OR3550 OR288 OR07 OR04 OR7 OR20 OR03 OR3 OR OR06 OR02 OR3465 OR3420 OR3379 OR3466 OR3392 OR3398 OR2892 OR3220 OR3247 OR2899 OR3542 OR2975 OR2 OR3249 OR2858 OR383 OR297 OR3273 OR2890 OR2862 OR3208 OR2868 OR3 OR3243 OR324 OR2976 OR2878 OR384 OR2686 OR2700 OR3397 OR3470 OR2707 OR2705 OR272 OR3004 OR3449 OR3468 OR2908 OR3236 OR3380 OR3435 OR3440 OR347 OR2952 OR2955 OR2888 OR3437 OR7 OR2925 OR297 OR296 OR2963 OR2965 OR2854 OR3422 OR3462 OR2973 OR29 OR3387 OR3476 OR2859 OR2967 OR3478 OR2872 OR2856 OR3229 OR5 OR342 OR6 OR344 OR2893 OR3228 OR344 OR3403 OR2897 OR2895 OR2853 OR3258 OR3022 OR272 OR3383 OR338 OR3453 OR2903 OR29 OR3489 OR3425 OR3444 OR34 OR3385 OR286 OR3275 OR8 OR3433 OR3408 OR3442 OR2905 OR399 OR2962 OR3233 OR3269 OR2879 OR3226 OR2974 OR3268 OR2870 OR386 OR2904 OR2877 OR387 OR2693 OR306 OR3456 OR3430 OR2 OR9 OR3464 OR3009 OR2682 OR322 OR2923 OR292 OR390 OR3544 OR3244 OR275 OR3076 OR2866 OR2958 OR325 OR2865 OR23 OR2863 OR294 OR3209 OR2966 OR3224 OR2884 OR397 OR3245 OR2959 OR2696 OR303 OR3206 OR295 OR2889 OR3276 OR3399 OR3434 OR2902 OR2864 OR3054 OR3025 OR2926 OR3230 OR3402 OR2857 OR2694 OR3026 OR3203 OR2894 OR3223 OR2907 OR2852 OR3545 OR3390 OR3482 OR32 OR2876 OR2887 OR329 OR3388 OR3473 OR296 OR3506 OR2855 OR3222 OR3432 OR OR388 OR287 OR2954 OR2957 OR346 OR3393 OR3474 OR3436 OR2882 OR2873 OR3443 OR3457 OR3458 OR3452 OR24 OR298 OR4 OR323 OR3242 OR3567 OR3005 OR304 OR2969 OR2968 OR3550 OR288 OR07 OR04 OR7 OR20 OR03 OR3 OR OR06 OR02 OR3465 OR3420 OR3379 OR3466 OR3392 OR3398 OR2892 OR3220 OR3247 OR2899 OR3542 OR2975 OR2 OR3249 OR2858 OR383 OR297 OR3273 OR2890 OR2862 OR3208 OR2868 OR3 OR3243 OR324 OR2976 OR2878 OR384 OR2686 OR2700 OR3397 OR3470 OR2707 OR2705 OR272 OR3004 OR3449 OR3468 OR2908 OR3236 OR3380 OR3435 OR3440 OR347 OR2952 OR2955 OR2888 OR3437 OR7 OR2925 OR297 OR296 OR2963 OR2965 OR2854 OR3422 OR3462 OR2973 OR29 OR3387 OR3476 OR2859 OR2967 OR3478 OR2872 OR2856 OR3229 OR5 OR342 OR6 OR344 OR2893 OR3228 OR344 OR3403 OR2897 OR2895 OR2853 OR3258 OR3022 OR272 OR3383 OR338 OR3453 OR2903 OR29 OR3489 OR3425 OR3444 OR34 OR3385 OR286 OR3275 OR8 OR3433 OR3408 OR3442 OR2905 OR399 OR2962 OR3233 OR3269 OR2879 OR3226 OR2974 OR3268 OR2870 OR386 OR2904 OR2877 OR387 OR2693 OR306 OR3456 OR3430 OR2 OR9 OR3464 OR3009 OR2682 OR322 OR2923 OR292 OR390 OR3544 OR3244 OR275 OR3076 OR2866 OR2958 OR325 OR2865 OR23 OR2863 OR294 OR3209 OR2966 OR3224 OR2884 OR397 OR3245 OR2959 OR2696 OR303 OR3206 OR295 OR2889 OR3276 OR3399 OR3434 OR2902 OR2864 OR3054 OR3025 OR2926 OR3230 OR3402 OR2857 OR2694 OR3026 OR3203 OR2894 OR3223 OR2907 OR2852 OR3545 OR3390 OR3482 OR32 OR2876 OR2887 OR329 OR3388 OR3473 OR296 OR3506 OR2855 OR3222 OR3432 OR OR388 OR287 OR2954 OR2957 OR346 OR3393 OR3474 OR3436 OR2882 OR2873 log(p value) 0 5 5 20 25 30 35 SFRP NTRK2 KIT SPRY2 LIFR ITGA7 MAML2 IGF ILRA PDGFD PPARG RUNXT LEPR CDKNC JUN CDKN2C ID4 XPA CCNA2 PKMYT UBE2T H2AFX CCNB EZH2 HISTH3H TTK INHBA E2F HISTH3B PTTG2 RAD5 CDC25C FANCA CCNE2 CDC6 MYD88 CDC25A 4 2 0 2 4 Log(fold change) ERBB2 COMP COLA p < 0.0 p < 0.00 3

CUSTOM CODESET DESIGN SELECT GENES 2 PROBE DESIGN 3 REVIEW 4 MANUFACTURE PROCESS DESCRIPTION Submit your RefSeq IDs for up to 800 target genes to NanoString. NanoString designs probes then creates and sends a Design Report. Customer reviews and approves Design Report. NanoString manufactures and ships CodeSet to customer. LEAD TIME Customer-defined. Custom Gene Expression: 3-5 days Custom CNV: -5 days Customer-defined. 3-5 weeks, depending on gene number and scale. MODIFY YOUR PROJECT WITH PANEL-PLUS AND CODESET-PLUS RESEARCH PRODUCTS ncounter Panel-Plus and CodeSet-Plus products add an extra level of flexibility to ncounter experiments. Researchers can customize any NanoString off-the-shelf panel kit or add genes to any custom CodeSet by utilizing up to 30 unique Reporter Probes exclusively formulated for use in Panel-Plus and CodeSet-Plus products. Plus products require minor modifications to sample hybridization protocols. After hybridization, all subsequent steps are performed exactly as described in the instrument manual. Customize a Panel Add up to 30 of additional genes or a collection of specific controls to make your panel unique to that experiment. Update a Large CodeSet Utilize results from your experiments and add new genes over time to keep your custom CodeSet updated. Create Specific Control Sets Use different Plus reagents with different samples or cell types. PANEL-PLUS REAGENTS Any off-the-shelf panel kit can be customized using Panel-Plus reagents. In addition, using a panel kit combined with Panel-Plus is less expensive compared to the same gene set ordered as a custom CodeSet. CODESET-PLUS REAGENTS Update custom CodeSets that may be used to analyze large sample sets over many months. Genes of interest that become known from ongoing research, or from a new publication, can be easily added to a custom CodeSet. Purchasing a larger custom CodeSet also provides a more cost-effective solution when compared to purchasing multiple smaller custom CodeSets. 4

READY FOR TRANSLATION CREATE YOUR OWN LABORATORY TESTS By developing and testing a potential signature with ncounter Elements reagents, investigators are able to share that knowledge with clinicians. ncounter Elements reagents are an evolution of our standard chemistry, providing generic Reporter Tags and a Universal Capture Tag that can be modified with the addition of short (~0 bp) oligonucleotide probes. REPORTER TAG Selected probes procured by end user UNIVERSAL CAPTURE TAG A unique tag sequence PROBE A** PROBE B** is assigned to each target sequence 5 3 NUCLEIC ACID TARGET ONE INSTRUMENT FROM LAB TO CLINIC The Prosigna Breast Cancer Prognostic Gene Signature Assay has received 5(k) clearance for use with the ncounter Dx Analysis System with FLEX configuration. ncounter FLEX is manufactured under GMP guidelines and ISO 3485 to ensure quality and compliance with international standards. In Life Sciences mode, ncounter FLEX can perform all ncounter Life Science and ncounter Elements assays for research use. Diagnostics mode provides a secondary interface for running diagnostic assays such as the Prosigna Breast Cancer Prognostic Gene Signature Assay. As NanoString s most flexible instrument configuration, ncounter FLEX ensures you ll be ready for anything. Process 2 to 92 samples per day with one Prep Station and one Digital Analyzer, depending on your desire for additional sample multiplexing. EXPERIENCE WITH CLINCIAL DIAGNOSTICS Our experience developing, testing, and marketing Prosigna demonstrates our commitment to establishing ncounter as a truly multipurpose platform for research and clinical use. DEVELOPMENT MANUFACTURING CLINICAL REGULATORY COMMERCIAL Assay development Reagent production Assay SOP transfer Pre-IDE Reimbursement planning Algorithm training Process validation Medical affairs IDE submission Marketing Software development GMP and ISO 3485 certification Site monitoring PMA submission Sales 5

NanoString Technologies nanostring.com info@nanostring.com.888.358.6266 JUNE 205 LBL-83-0 Research Use Only. Not for use in diagnostic procedures. 205 NanoString Technologies, Inc. All rights reserved. NanoString, NanoString Technologies, ncounter, mirge, and nsolver are registered trademarks or trademarks of NanoString Technologies, Inc., in the United States and/or other countries. PAXgene is a trademark of QIAGEN. Windows, Macintosh, and other branded product names are registered trademarks or trademarks of their respective companies.