HUMAN CELL CULTURE Volume VII: Human Adult Stem Cells

HUMAN CELL CULTURE Volume VII: Human Adult Stem Cells

Human Cell Culture Volume 7 For futher volumes: http://www.springer.com/series/6058

Human Cell Culture Volume VII Human Adult Stem Cells edited by John R. Masters University College London, London, UK and Bernhard Ø. Palsson University of California, San Diego, CA, USA 123

Editors Dr. John R. Masters University College London 67 Riding House Street London United Kingdom W1W 7E j.masters@ucl.ac.uk Prof. Bernhard Ø. Palsson University of California, San Diego Dept. Bioengineering 9500 Gilman Dr. La Jolla CA 92093-0412 USA bpalsson@bioeng.ucsd.edu ISSN 1389-2142 ISBN 978-90-481-2268-4 e-isbn 978-90-481-2269-1 DOI 10.1007/978-90-481-2269-1 Springer Dordrecht Heidelberg London New York Library of Congress Control Number: 2009926880 c Springer Science+Business Media B.V. 2009 No part of this work may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording or otherwise, without written permission from the Publisher, with the exception of any material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Cover design: Boekhorst Design BV Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)

Preface The aim of this volume is to provide protocols for isolating and maintaining human adult stem cells in culture and methods for directing differentiation into specialized cell types. Human adult stem cells from each tissue are potentially capable of differentiating into all the cell types within that tissue. Consequently there is considerable interest in using these cells for the treatment of a wide variety of medical conditions. Careful consideration needs to be given to the relative value of embryonic (described in Human Cell Culture Volume 6), induced pluripotential stem cells and adult stem cells. The development and optimization of techniques for growing human adult stem cells are crucial for the clinical application of these cells and are the focus of this volume. v

Contents 1 Neural Progenitors... 1 Dustin R. Wakeman, Martin R. Hofmann, Yang D. Teng and Evan Y. Snyder 2 Multipotent Stromal Cells (hmscs)... 45 Margaret Wolfe, Alan Tucker, Roxanne L. Reger and Darwin J. Prockop 3 Endothelium... 73 Sangmo Kwon and Takayuki Asahara 4 Lung... 91 Rabindra Tirouvanziam, Megha Makam and Bruno Péault 5 Eye...113 Maria Notara, Yiqin Du, G. Astrid Limb, James L. Funderburgh and Julie T. Daniels 6 Colon...143 F. Iovino, Y. Lombardo, V. Eterno, P. Cammareri, G. Cocorullo, M. Todaro and G. Stassi 7 Spermatogonia...157 Makoto C. Nagano, Jonathan R. Yeh and Khaled Zohni 8 Hair Follicle Pluripotent Stem (hfps) Cells...171 Robert M. Hoffman vii

viii Contents 9 Pancreas...183 Fang-Xu Jiang and Grant Morahan 10 Prostate...197 C. Foley, K.T. Brouilette, C. Kane, H. Patel, H. Yamamoto and A. Ahmed

Contributors A. Ahmed Division of Surgery and Interventional Sciences, Charles Bell House Laboratories, Prostate Cancer Research Centre, University College London, 67 Riding House Street, London, UK, aamir.ahmed@ucl.ac.uk Takayuki Asahara Department of Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan, asa777@aol.com K. T. Brouilette Division of Surgery and Interventional Sciences, Charles Bell House Laboratories, Prostate Cancer Research Centre, University College London, 67 Riding House Street, London, UK, k.brouilette@ucl.ac.uk P. Cammareri Department of GENURTO, University of Palermo, Palermo, Italy, patrizia.cammareri@gmail.com G. Cocorullo Department of GENURTO, University of Palermo, Palermo, Italy, gianfranco.cocorullo@unipa.it Julie T. Daniels UCL Institute of Ophthalmology, 11-43 Bath Street, EC1V 9EL, London, UK, j.daniels@ucl.ac.uk Yiqin Du Department of Ophthalmology, School of Medicine, UPMC Eye Center, University of Pittsburgh, Pittsburgh, PA 15213, USA, yiqindu@pitt.edu V. Eterno Cellular and Molecular Pathophysiology Laboratory, Department of Surgical and Oncological Sciences, University of Palermo, Palermo, Italy, eternovincenzo@libero.it C. Foley Division of Surgery and Interventional Sciences, Charles Bell House Laboratories, Prostate Cancer Research Centre, University College London, 67 Riding House Street, London, UK, charlotte foley@hotmail.com James L. Funderburgh Department of Ophthalmology, School of Medicine, UPMC Eye Center, University of Pittsburgh, Pittsburgh, PA 15213, USA, jlfunder@pitt.edu Robert M. Hoffman Department of Surgery, University of California, AntiCancer, Inc., 7917 Ostrow Street, San Diego, CA 92111, USA, all@anticancer.com ix

x Contributors Martin R. Hofmann The Burnham Institute for Medical Research, La Jolla, CA 92037, USA F. Iovino Cellular and Molecular Pathophysiology Laboratory, Department of Surgical and Oncological Sciences, University of Palermo, Palermo, Italy, flora.iovino@gmail.com Fang-Xu Jiang Western Australian Institute for Medical Research Centre for Diabetes Research and Centre for Medical Research, The University of Western Australia, 50 Murray St (Rear), Perth, WA 6000, Australia, jiang@waimr.uwa.edu.au C. Kane Division of Surgery and Interventional Sciences, Charles Bell House Laboratories, Prostate Cancer Research Centre, University College London, 67 Riding House Street, London, UK, c.kane@ucl.ac.uk Sangmo Kwon Department of Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan G. Astrid Limb UCL Institute of Ophthalmology, 11-43 Bath Street, EC1V 9EL, London, UK, g.limb@ucl.ac.uk Y. Lombardo Cellular and Molecular Pathophysiology Laboratory, Department of Surgical and Oncological Sciences, University of Palermo, Palermo, Italy, ylela@hotmail.com Megha Makam Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305-5318, USA, meghasmakam@gmail.com Grant Morahan Western Australian Institute for Medical Research, and Centre for Medical Research, Centre for Diabetes Research, The University of Western Australia, Perth 6000, Australia, gem@wairmr.uwa.edu.au Makoto C. Nagano Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Quebec H3A 1A1, Canada, makoto.nagano@muhc.mcgill.ca Maria Notara UCL Institute of Ophthalmology, 11-43 Bath street, EC1V 9EL, London, UK, m.notara@ucl.ac.uk H. Patel Division of Surgery and Interventional Sciences, Charles Bell House Laboratories, Prostate Cancer Research Centre, University College London, 67 Riding House Street, London, UK, hrhpatel@hotmail.com Bruno Péault Departments of Pediatrics and Cell Biology, Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, PA 15213, USA, bruno.peault@chp.edu Darwin J. Prockop Institute for Regenerative Medicine at Scott and White, Texas A&M Health Sciences Center, Temple, TX, 76502, USA

Contributors xi Roxanne L. Reger Institute for Regenerative Medicine at Scott and White, Texas A&M Health Sciences Center, Temple, TX, 76502, USA Evan Y. Snyder The Burnham Institute for Medical Research, University of California at San Diego, Graduate Program in Biomedical Sciences, 9500 Gilman Dr., La Jolla, CA 92093, USA, esnyder@burnham.org G. Stassi Cellular and Molecular Pathophysiology Laboratory, Department of Surgical and Oncological Sciences, University of Palermo, Palermo, Italy, gstassi@tiscali.it Yang D. Teng Departments of Neurosurgery, and Physical Medicine and Rehabilitation, Harvard Medical School, Brigham and Women s Hospital and Spaulding Rehabilitation Hospital; Division of Spinal Cord Injury Research, Veterans Affairs Boston Healthcare System, Boston, MA 02130, USA Rabindra Tirouvanziam Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305-5318, USA, tirouvan@stanford.edu M. Todaro Cellular and Molecular Pathophysiology Laboratory, Department of Surgical and Oncological Sciences, University of Palermo, Palermo, Italy, matilde.todaro@tiscali.it Alan Tucker Center for Gene Therapy, Tulane University Health Sciences, New Orleans, LA, 70112, USA, atucker@tulane.edu Dustin R. Wakeman The Burnham Institute for Medical Research, University of California at San Diego, Graduate Program in Biomedical Sciences, 9500 Gilman Dr., La Jolla, CA 92093, USA, dwakeman@burnham.org Margaret Wolfe Center for Gene Therapy, Tulane University Health Sciences, New Orleans, LA, 70112, USA, peggiwolfe@bellsouth.net H. Yamamoto Division of Surgery and Interventional Sciences, Charles Bell House Laboratories, Prostate Cancer Research Centre, University College London, 67 Riding House Street, London, UK, h.yamamoto@ucl.ac.uk Jonathan R. Yeh Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Quebec H3A 1A1, Canada Khaled Zohni Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Quebec H3A 1A1, Canada