Discover EXBIO Milestones

May 2015 information magazine for partners EXBIO Praha, a.s. www.exbio.cz Our Mission EXBIO Praha strives to exceed the most demanding customer expectations in the field of analytical cytometry by providing a comprehensive portfolio of high quality products and services at affordable prices. Discover EXBIO Milestones We are a manufacturing company with strong monoclonal antibody experience, focused on design, development and distribution of flow cytometry products for both in vitro diagnostic and research use. In this special edition of our bulletin we would like to introduce you with a breaf overwiew of our history. It all began at the end of 70 s in former Czechoslovakia, when researchers at the Institute of Molecular Genetics in Prague gained great experiences with construction of monoclonal antibodies. The hybridoma technology there quickly reached high quality. The antibody TU 01, prepared in the spring of 1980, was one of the first reagents in the world specifically recognizing alpha-tubulin in cytoskeletal structures, and the number of developed and characterized unique monoclonal antibodies continuously increased. The management of the Institute was aware not only of scientific, diagnostic and therapeutic impact of the developed antibodies, but also appreciated their economic potential. As a result, a Technology unit was established to develop and produce monoclonal antibodies to meet the demands of national industry. In 1990, soon after the collaps of the communist system, this Technology unit, including hybridoma cell lines, was transformed into an independent company EXBIO. In 1994, EXBIO successfully privatized the former biotechnolgy plant for manufacture of vaccines in Olomouc, but later, with the beginning of 1999, these two divisions were transformed into independent companies. Since that EXBIO Praha (the former Prague division) focused solely on the manufacture of monoclonal antibodies. The mission became to exceed the most demanding customer expectations in the field of analytical cytometry by providing a comprehensive portfolio of high quality products and services at affordable prices. Significant increase in manufacture and sales allowed the investment in building up a brand new facility (800 m 2 ) with its own animal house, cryo preservation site and biological and biochemical laboratories, which was opened in 2003. In September 2010 we experienced another great milestone in the history of our company. We opened the Research and Technology Centre EXBIO (2 200 m 2 ), co funded by European regional development fund (ERDF) under call of Czech Ministry of Industry Potential. Participation in various research grants enabled us to broadly expand our research activities focused on development of new products, which would better reflect the demands of our customers. In late September 2014 we opened new EXBIO Manufacturing Centre (1 100 m 2 ). Construction of these facilities and the purchase of new technologies were forced by the ever growing demand for the company s products from around the world. Building on the style and functionality of the current installations, the new manufacturing facility was designed to enable production in compliance with international standards. Funding from the EU Structural Funds under the Operational Programme Enterprise and Innovation under the Innovation Programme helped the company to invest in the new facilities and technologies. This high tech workplace has generated new highly specialised job positions, and we expect roughly a tenfold increase in production capacity. We realize every day that our achievements would have not been possible without continuous support of our customers and we would like to thank to all of you. 1

HOT Topics Newly Added s With respect to our customers needs we are continuously searching fo valuable hybridomas to supplement our portfolio with high quality antibody clones, and we are also developing our own ones. Here we bring a table of clones that have been added to our portfolio since last issue. As your needs and requirements are of high importance for us, we are grateful for your feedback what other important clones you would like to see in our portfolio. Catalog No. Host Isotype Reactivity Application Format Quantity 11-155-C025 11-155-C100 Ki-67 Ki-67 Ms IgG1 Hu, Bov FC, WB, IHC, ICC A4-155-T025 A4-155-T100 1P-155-T025 1P-155-T100 A6-155-T025 A6-155-T100 11-160-C025 11-160-C100 CD45 2D1 Ms IgG1 Hu FC 11-770-C025 11-770-C100 CD144 / VE-Cadherin 55-7H1 Ms IgG1 Hu FC, IP, WB, IHC(F), ICC 11-783-C025 11-783-C100 CD146 P1H12 Ms IgG1 Hu, Ms, Can, Rb FC, IP, WB, IHC(P), IHC(F) 1P-783-T025 1P-783-T100 11-775-C025 11-775-C100 CD158a/g/h HP-MA4 Ms IgG2b Hu FC, IP 11-142-C025 11-142-C100 IgD IA6-2 Ms IgG2a Hu FC 1P-142-T025 1P-142-T100 11-719-C025 11-719-C100 NR2F6 EM-51 Ms IgG2a Hu WB Regulatory status: RUO Ki 67: Proliferation Marker Ki 67 is a highly protease sensitive nuclear protein expressed in two isoforms (345 kda and 395 kda), both of which are identified by the antibody clone Ki 67. The Ki 67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki 67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies. Immunocytochemistry detection of Ki-67 in U2OS cell line (human osteosarcoma) using monoclonal antibody Ki-67 (green). Cell nuclei stained with DAPI (blue). Flow cytometry analysis of human peripheral blood mononuclear cells stimulated with PHA. Surface staining of CD25 (clone MEM-181 APC) was followed by permeabilization and nuclear staining of Ki-67 (clone Ki-67 ). 2

Flow Cytometry Top Sellers Here we bring list of our most popular products for flow cytometry application, divided according to the topics. The related products you can find on the following pages. Human flow cytometry Catalog no. Host Isotype Format Quantity PB-514-T100 CD3 UCHT1 Mouse IgG1 Pacific Blue 1F-691-T100 CD5 L17F12 Mouse IgG2a FITC T9-691-T100 CD5 L17F12 Mouse IgG2a PerCP-Cy 5.5 PB-208-T100 CD9 MEM-61 Mouse IgG1 Pacific Blue PB-646-T100 CD16 3G8 Mouse IgG1 Pacific Blue T7-646-T100 CD16 3G8 Mouse IgG1 -Cy 7 PB-306-T100 CD21 LT21 Mouse IgG1 Pacific Blue 1A-577-T100 CD28 CD28.2 Mouse IgG1 APC 1P-273-T100 CD31 MEM-05 Mouse IgG1 PC-222-T100 CD45 MEM-28 Mouse IgG1 PerCP 1F-498-T100 CD45R0 UCHL1 Mouse IgG2a FITC 1P-644-T100 CD64 10.1 Mouse IgG1 1P-530-T100 CD79a HM57 Mouse IgG1 1F-670-T100 CD103 Ber-ACT8 Mouse IgG1 FITC 1P-586-T100 CD117 104D2 Mouse IgG1 1A-586-T100 CD117 104D2 Mouse IgG1 APC T7-586-T100 CD117 104D2 Mouse IgG1 -Cy 7 PO-520-T100 CD138 B-A38 Mouse IgG1 Pacific Orange PB-674-T100 Kappa light chains TB28-2 Mouse IgG1 Pacific Blue T9-690-T100 HLA-DR L243 Mouse IgG2a PerCP-Cy 5.5 T7-690-T100 HLA-DR L243 Mouse IgG2a -Cy 7 Rodent immunology Catalog no. Host Isotype Format Quantity 1F-580-C100 CD4 (mouse) GK1.5 Rat IgG2b FITC 1F-579-C100 CD8a (mouse) 53-6.7 Rat IgG2a FITC 1P-579-C100 CD8a (mouse) 53-6.7 Rat IgG2a 1F-595-C100 CD11b (mouse, human) M1/70 Rat IgG2b FITC 1P-614-C100 CD25 (mouse) PC61.5 Rat IgG1 1P-601-C100 FoxP3 (mouse, human) 3G3 Mouse IgG1 1F-679-C100 CD4 (rat) OX-35 Mouse IgG2a FITC 1F-656-C100 CD8b (rat) 341 Mouse IgG1 FITC 1P-655-C100 CD28 (rat) JJ319 Mouse IgG1 Flow cytometry kits Catalog no. Product name Description Quantity ED7043 BasoFlowEx Kit Examination of allergy by basophil activation test (BAT) ED7042 FagoFlowEx Kit Quantification of phagocytic activity by measuring the oxidative burst Regulatory status: RUO 3

Human Immunology Products: Flow Cytometry Human CD and Related s CD1a HI149 CD1b SN13 CD1c L161 CD2 LT2 MEM-65 TS1/8 CD3 MEM-57 MEM-92 OKT3 UCHT1 CD3 (activation epitope) APA1/1 CD3 zeta (CD247) H146-968 CD3 zeta (Phospho Tyr111) EM55 CD3 zeta (Phospho Tyr142) EM-54 CD3 zeta (Phospho Tyr153) EM-17 CD3 zeta (Phospho Tyr72) EM-26 CD4 MEM-115 MEM-16 MEM-241 CD5 CRIS1 L17F12 MEM-32 CD6 MEM-98 CD7 MEM-186 CD8 MEM-31 MEM-87 CD9 MEM-61 CD10 LT10 MEM-78 CD11a MEM-25 MEM-83 CD11b ICRF44 MEM-170 MEM-174 CD11c CD13 CD14 BU15 WM15 B-A8 MEM-15 MEM-18 CD15 MEM-158 CD16 3G8 LNK16 MEM-154 MEM-168 CD17 CD18 CD19 CD20 MEM-68 MEM-148 MEM-48 4G7 LT19 2H7 LT20 MEM-269 MEM-97 CD21 LT21 CD22 IS7 MEM-01 CD23 EBVCS-5 CD24 SN3 CD25 MEM-140 MEM-181 CD26 BA5b CD27 LT27 CD28 CD28.2 CD29 MEM-101A CD30 MEM-268 CD31 MEM-05 CD32 3D3 CD33 HIM3-4 WM53 biotin Pacific Blue FITC Pacific Orange -DyLight 594 APC -Cy 5 PerCP PerCP-Cy 5.5 Alexa Fluor 700 -Cy 7 APC-Cy 7 4

Here you can find a table of our human immunology antibody conjugates. For rodent immunology and other related topics, as well as for the most up to date information, please visit our web www.exbio.cz. Human CD and Related s biotin Pacific Blue FITC Pacific Orange -DyLight 594 APC -Cy 5 PerCP PerCP-Cy 5.5 Alexa Fluor 700 -Cy 7 APC-Cy 7 CD34 CD35 CD36 4H11[APG] 581 QBEnd-10 E11 CB38 (NL07) TR9 CD38 HIT2 CD40 HI40a CD41 MEM-06 CD41a HIP8 CD42a GR-P CD41b HIP2 CD42b HIP1 CD43 MEM-59 CD44 IM7 MEM-263 MEM-85 CD45 HI30 MEM-28 2D1 CD45R0 UCHL1 CD45RA MEM-56 CD45RB MEM-143 MEM-55 CD46 MEM-258 CD47 CD48 CD50 CD52 CD53 CD54 MEM-122 MEM-102 MEM-04 MEM-171 HI186 MEM-53 1H4 MEM-111 MEM-112 CD55 MEM-118 CD56 MEM-188 LT56 CD58 MEM-63 CD59 MEM-129 MEM-43 MEM-43/5 CD61 VIPL2 CD62L DREG56 LT-TD180 CD62P AK4 HI62P CD63 MEM-259 CD64 10.1 CD66acde CLB-gran/10 CD66e CB30 CD68 Y1/82A CD69 FN50 CD71 MEM-189 MEM-75 CD72 3F3 CD73 AD2 CD79a HM47 HM57 CD79b CB3-1 CD80 MEM-233 CD81 M38 CD82 C33 CD83 HB15e CD84 CD84.1.21 CD85j GHI/75 CD86 BU63 CD88 S5/1 CD90 5E10 CD94 HP-3D9 5

Human Immunology Products: Flow Cytometry Human CD and Related s CD95 / Fas LT95 UT-1 CD97 MEM-180 CD98 MEM-108 CD99 3B2/TA8 CD99R MEM-131 CD101 BB27 CD103 Ber-ACT8 CD105 MEM-226 MEM-229 CD106 STA CD107a B-T47 H4A3 CD107b H4B4 CD108 MEM-150 CD112 R2.525 CD116 4H1 CD117 104D2 CD120a H398 CD123 6H6 CD129 AH9R7 CD135 BV10A4 CD137 4B4-1 CD138 B-A38 CD140a / PDGF-RA 16A1 CD140b / PDGF-RB 18A2 CD144 55-7H1 CD146 P1H12 CD147 MEM-M6/1 MEM-M6/2 MEM-M6/6 CD148 MEM-CD148/05 CD150 SLAM.4 CD152 BNI3 CD157 SY11B5 CD158a/g/h HP-MA4 CD158d / KIR2DL4 mab#33 CD158f UP-R1 CD160 BY55 CD161 HP-3G10 CD162 TC2 CD163 GHI/61 CD169 7-239 CD177 MEM-166 CD178 NOK-1 CD180 G28-8 CD195 T21/8 CD200 OX-104 CD203c NP4D6 CD205 HD30 CD209 UW60.1 CD222 MEM-238 CD229 HLy9.25 CD235a HIR2 JC159 CD253 / TRAIL 2E5 CD255 / TWEAK CARL-1 CD261 / TRAIL-R1 DR-4-02 CD262 / TRAIL-R2 DR5-01-1 CD263 / TRAIL-R3 TRAIL-R3-02 CD264 / TRAIL-R4 TRAIL-R4-01 CD266 / TWEAK R ITEM-4 CD271 NGFR5 CD300a MEM-260 CD300e UP-H2 CD305 NKTA255 CD314 1D11 CD324 / E-Cadherin 67A4 CD326 / EpCAM VU-1D9 biotin Pacific Blue FITC Pacific Orange -DyLight 594 APC -Cy 5 PerCP PerCP-Cy 5.5 Alexa Fluor 700 -Cy 7 APC-Cy 7 6

Here you can find a table of our human immunology antibody conjugates. For rodent immunology and other related topics, as well as for the most up to date information, please visit our web www.exbio.cz. Human CD and Related s biotin Pacific Blue FITC Pacific Orange -DyLight 594 APC -Cy 5 PerCP PerCP-Cy 5.5 Alexa Fluor 700 -Cy 7 APC-Cy 7 CD328 Siglec-7 6-434 CD358 / DR6 DR-6-04-EC CD361 MEM-216 TCR alpha/beta IP26 TCR beta (Vbeta5.3-related) MEM-262 STRO-1 STRO-1 beta2-microglobulin B2M-01 B2M-02 HLA-Class I MEM-123 W6/32 MEM-147 MEM-81 HLA-A2 BB7.2 HLA-B7 BB7.1 HLA-E MEM-E/06 MEM-E/07 MEM-E/08 HLA-G MEM-G/9 MEM-G/11 5A6G7 2A12 87G 01G G233 4H84 HLA-DQ1+DQ3 HL-37 HLA-DR HL-39 MEM-12 L243 HLA-DR1 (empty) MEM-267 HLA-DR+DP MEM-136 HL-38 HL-40 Immunoglubulins IgD IgM IgG(Fab) IgE Kappa light chains IA6-2 CH2 4A11 BE5 4H10 TB28-2 A8B5 MEM-09 Lambda light chains 1-155-2 4C2 Important Intracellular Markers FoxP3 (Hu, Ms) Helios Granzyme B (Hu) Myeloperoxidase (Hu) TNF-alpha IFN-gamma Isotype Controls 3G3 22F6 CLB-GB11 MPO421-8B2 MAb11 4S.B3 G-23 Mouse IgG1 Isotype Control MOPC-21 Mouse IgG2a Isotype Control MOPC-173 Mouse IgG2b Isotype Control MPC-11 Mouse IgG3 Isotype Control PPV-07 Mouse IgM Isotype Control PFR-03 7

Miroslav Benko, CCy Product Development Manager Martin Špryngar, CCy Quality Control Specialist Where you can meet us: International School on Cytometry Advanced Applications in Hematology 2015 May 25 30, 2015 Prague, Czech Republic CYTO 2015 June 26 30, 2015 Glasgow, Scotland ECI 2015 September 6 9, 2015 Wien, Austria ESCCA 2015 September 30 October 3 Naxos, Sicilia Medica 2015 November 16 19, 2015 Düsseldorf, Germany The Importance of Being CCy (Not) a Trivial Exam for Serious Flowers Flow cytometry, as an interdisciplinary analytical method combining physics, chemistry, biology, and statistics, undergoes a dynamic continuous development. The International Cytometry Certification Examination (ICCE) was established as the result of collaboration between ISAC, ICCS, and ESCCA, with support from the Wallace H. Coulter Foundation and with valuable input from cytometrists in many locations and work settings. Becoming a Certified Cytometrist (entitled to use the designation CCy or C. Cy. after their names) tells the scientific community that you have true flow cytometry expertise. (For more information see http://cytometrycertification.org) We are proud that the first two Certified Cytometrists in the Czech Republic and Slovakia are employees of EXBIO. Miroslav Benko, CCy works as the product development manager, and Martin Špryngar, CCy is the quality control specialist. Their presence in our company ensures that EXBIO flow cytometry core facility performs all cytometry applications accurately and any problems are correctly troubleshooted. Last but not least our customers can be sure that they will always receive the highest quality support in the field of flow cytometry. Abbreviations APC allophycocyanin FITC fluorescein isothiocyanate R-phycoerythrin PerCP peridinin chlorophyl a protein RUO research use only CE-IVD in conformity with the European In Vitro Diagnostic Medical Device Directive 98/79/EC. Quality statement Our company has been assessed and certified as meeting the requirements of ISO 9001:2008, ISO 14001:2004, and ISO 13485: 2003 for the following activities: Design, development and production of biotechnological products for research use. Design, development and production of in vitro diagnostics for cell and protein analysis. Alexa Fluor, Pacific Blue and Pacific Orange are trademarks of Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corporation); DyLight is a trademark of Thermo Fisher Scientific; Cy is a trademark of GE Healthcare Limited. EXBIO Praha, a.s. Nad Safinou II 341 252 42 Vestec u Prahy fax +420 261 090 660 orders@exbio.cz technical@exbio.cz www.exbio.cz 8