Grow it. Read it. See it. Count it. imaging reader

Grow it. Read it. See it. Count it. imaging reader

Cytation 3 combines automated digital widefield microscopy and conventional multi-mode microplate detection in a unique patent pending system designed to capture rich phenotypic information and collect meaningful quantitative data in a single platform. Incorporating a powerful fluorescence and brightfield imaging module with the patented Hybrid Technology for multi-mode detection provides a streamlined workflow for cell-based and biochemical assays without the complexity and expense that is typical of automated microscopes and multi-mode detection systems. The modular architecture allows users to customize Cytation3 to suit their needs now and upgrade when assay and application requirements change in the future. The image on the reverse side is BPAEC cells from FluoCells prepared slide #2, captured on Cytation3 at 4x magnification. FluoCells is a registered trademark of Life Technologies, Inc.

Cytation3 Cell Imaging Multi-Mode Reader Automated Microscopy & Multi-Mode Detection Fully Automated Imaging System Auto X Y stage simplifies precise sample positioning and stage movement Auto LED controls up to 100% intensity output Auto exposure applies an average exposure setting across the plate Auto focus determines the plane of focus automatically based on plate or sample vessel dimensions Multi-Mode Microplate Detection The microplate reader in Cytation3 measures fluorescence intensity, time resolved fluorescence and fluorescence polarization along with luminescence and UV-Vis absorbance. The patented Hybrid Technology brings filter-based and monochromator-based optics together to provide the powerful detection capabilities required for cell-based and other biochemical assays. Multiple parallel detectors speed the measuring time, and the dedicated filter-based optics are optimized for live cell assays. Modular & Upgradable Cytation3 has a modular architecture, so it s easy to select any combination of the independent systems now, and upgrade as the laboratory s needs change. Monochromator optics Filter optics Imaging camera and optics

Grow It Cytation3 can be outfitted with a CO 2 / O 2 control module to provide the optimal environment for cell growth, along with programmable temperature control to 45 ºC, plus orbital and linear shaking. An available dual reagent dispenser is a valuable tool for precise reagent dispensing - ideal for dispense/read operations. See It There s never been an easier or faster way to image your cells than in Cytation3! The versatile imaging system provides up to four fluorescent color channels plus brightfield imaging, making it the ultimate benchtop imaging workstation. Gen5 software offers Auto Easy functions for all steps of imaging, from managing focus to LED intensity for users at all levels (manual operation is also available). Excellent image quality is assured with the 16 bit grayscale CCD camera with Sony chip, along with other high quality optical components. Read It Plate measurement options are nearly limitless in Cytation3. The multi-mode microplate detection system offers fluorescence intensity, time-resolved fluorescence, fluorescence polarization, UV-Vis absorbance, flash and glow luminescence. All modes are available for measuring samples in 6- to 384-well plates to suit a wide range of applications. If cell imaging is the next step in your workflow, Gen5 offers a hit-picking function to select just the wells whose intensity meets the imaging threshold. Saves time and greatly reduces data analysis and storage requirements. Count It Cytation3 is controlled by Gen5 software for quantitative and image data collection and analysis. Cell counting in microplates is as easy as clicking here and there. Cell counts and image statistics are automated calculated, and analysis options are intuitive and powerful, whether the samples are in microplates or slides.

NIH3T3 cells 20x Image Analysis Made Easy Gen5 software automates the entire imaging process and makes cellular image analysis simple and intuitive. Cell counting and sub-population data are calculated as images are acquired, and Gen5 s data analysis tools are easily applied. It s powerful software for imaging and microplate analysis, but is designed for users of all skill levels, including those without prior imaging experience. Captured images are analyzed, optimized, displayed and quickly and output in a variety of formats for printing, exporting and saving. Gen5 can import.tif files from other imaging software as well, for added convenience. Purposeful Image Statistics General statistics are calculated on the image s pixel values, providing relevant information like average pixel intensity and standard deviation. Show correlation between methods, confirm a hit, calculate the dose response or analyze migration behavior, all within the automated or manual Image Statistics tools in Gen5. Cell Counting in Minutes NIH3T3 cells 20x (zoomed) It takes only a few minutes, not hours, to count cell nuclei in all the wells of a microplate. Imagine the improved workflow and time savings Cytation3 and Gen5 can offer, especially when testing multiple conditions and monitoring cell populations in microplates. Zebrafish-4x Image courtesy of Jeff Mumm at Georgia Regents University.

Sub-Population Analysis When multiple cell sub-populations are present in a sample, Gen5 can sort by intensity or morphology, thus enabling analysis of transfection efficiency, nuclear translocation or cell cycle assays. Efficient Workflow Whether the assay is conducted in microplate, slide, petri dish or in the lowvolume Take3 plate, Cytation3 and Gen5 simplify the workflow by offering multi-mode detection, imaging and analysis in one platform. Protocols for measuring endpoint or kinetics, or imaging an entire well or montage in a well, along with many other assay steps, are easily created in the Gen5 procedure steps for efficiency, speed and optimal assay performance. Universal Image Format Gen5 images are compatible with many image analysis software programs including Image J or Cell Profiler for custom analysis. Raw data is saved in native grayscale.tif format, while presentation images may be saved in color in.tif,.jpg,.png,.gif,.bmp and.emf formats. Multi-Mode Detection Data Analysis Use the intuitive Gen5 interface to control Cytation3, collect data and analyze the data with the powerful tools available for qualitative and quantitative assays. Kinetic analysis, powerful curve-fitting options, EC 50 and potency calculations are among the many tools available in the software to get the results you need fast!

One Instrument. Many Applications. Cytation3 combines digital widefield microscopy and multi-mode detection for a more complete picture than either separate technology. This unique combination offers quantitative and phenotypic rich qualitative data in parallel, and when combined with specialized, optional accessories, the range of biochemical, live cell and fixed cell applications is almost endless. Cell imaging, analysis, proliferation and toxicity Cell counting Biomarker quantification Drug discovery Genetic analysis Drug absorption and metabolism Environmental testing Food safety Nucleic acid and protein quantification For more applications, visit www.cytation3.com Multiplexed Assay for IL-6 Secretion and Cell Viability Using an Epithelial Ovarian Cancer Using the Cytation3 Cell Imaging Multi-Mode Microplate Reader to Monitor IL-6 Secretion using HTRF detection and Cell Viability Using Digital Widefield Fluorescence Microscopy SKOV-3 cells; blue is DAPI-stained nuclei, green is Phalloidin actin staining. Automated Tissue Culture Cell Fixation and Staining in Microplates Using the EL406 Combination Washer Dispenser to Wash, Fix, Permeabilize, Stain and Add Antibody U-2 OS cells stained for mitochondria, F-actin and nucleus. Imaging of BacMam Transfected Human U-2 OS Cells in Microplates Optimization of Tranfection Conditions Using the Cytation3 Cell Imaging Multi-Mode Microplate Reader and Gen5 Data Analysis Software Imaging of BacMam Transfected Human U-2 OS Cells. Analysis of Nuclear Stained Cells Using the Cytation3 Cell Imaging Multi-Mode Microplate Reader with DAPI-stained Cells Use of Gen5 software s Split touching objects on cell counting. Scale bar indicates 10 μm.

High Quality Imaging. High Quality Components. Cytation3 unites BioTek s microplate instrumentation expertise with components from leading imaging experts. This combination provides unsurpassed workflow efficiency and a truly unique live cell assay solution. Olympus and Zeiss Objectives LED Cubes and Filter Cubes with Semrock Filters Dual Reagent Dispenser Gas Controller Module Cytation3 and BioStack Microplate Stacker Specifications General Imaging mode Detection mode Read method Labware type Temperature control Shaking Automation CO 2 and O 2 control Software Imaging System Camera Imaging filter cube capacity Imaging filter cubes available Imaging LED cubes available Objective capacity Available objectives Image collection rate Fluorescence Intensity Sensitivity Light source selection range Dynamic range Detection system Fluorescence and brightfield Monochromators: FL, Lum, UV-Vis Abs Filters: FL, TRF, FP, Lum Endpoint, time-lapse, kinetic, well mode, montage 6- to 384- well microplates, microscope slides, cell culture flasks (T25), Compatible with Take3 Micro-Volume Plates with 2 µl microspots To 45 C +0.5 C at 37 C Linear, orbital, double orbital BioStack and 3rd party automation compatible 0-20% CO 2 control and 1-19% O 2 control, with optional Gas Controller Gen5 Data Analysis Software included 16-bit gray scale, Sony CCD, 1.1 megapixel 4 onboard, user-replaceable filter cubes DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5 and CY7 365 nm, 465 nm, 590 nm, 523 nm, 505 nm, 623 nm, 740 nm 2 onboard user-replaceable objectives 2x, 2.5x, 4x, 10x, 20x, 40x, 60x 96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 3 colors, 4x, 12 minutes Monochromators: Top: fluorescein 2.5 pm typical (0.25 fmol/well 384-well plate) Bottom: fluorescein 5 pm typical (0.5 fmol/well 384-well plate) Filters/mirrors: Fluorescein 1 pm typical (0.1 fmol/well 384-well plate) Xenon flash lamp Double grating monochromators (top/bottom) and, Deep blocking bandpass filters/dichroic mirrors (top) Monochromators: 250-700 nm Filters: 200-700 nm (850 option) 5 decades Two PMT detectors: one for monochromator system, one for filter system Luminescence Sensitivity range Dynamic range Monochromator system: 20 amol ATP typical (flash) Filter system: 10 amol ATP typical (flash) 300-700 nm 6 decades Fluorescence Polarization Sensitivity range 5 mp at 1 nm fluorescein typical 320-700 nm (850 nm option) Time-Resolved Fluorescence Sensitivity Light source range Absorbance Light source selection range Bandpass Dynamic range Resolution Europium 100 fm typical with filters (10 amol/ well in 384-well plate) Europium 1200 fm typical with monos (120 amol/well in 384-well plate) Xenon flash lamp Monochromators: 250-700 nm Filters: 200-700 nm (850 option) Xenon flash lamp Monochromator 230-999 nm, 1 nm increment 4 nm (230-285 nm), 8 nm (>285 nm) 0-4.0 OD 0.0001 OD Reagent Dispensers Number Dispense volume Dead volume Plate geometry Dispense precision Dispense accuracy 2 syringe pumps 5-1000 µl in 1 µl increment 1 ml, 100 µl with back flush 6- to 384-well microplates <2% at 50-200 µl ±1 µl or 2% Physical Characteristics Power Dimensions Weight Regulatory 130 Watts max. 20 D x 16.5 W x 17.5 H (50.8 cm x 41.91 cm x 44.5 cm) 80 lbs (36.3 Kg) Specifications are subject to change. CE and TUV marked. Models for In Vitro Diagnostic use are available.

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