CHAPTER 2-2 PROTOZOA: CILIOPHORA AND HELIOZOA DIVERSITY



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Glime, J. M. 2013. Protozoa: Ciliophora and Heliozoa Diversity. Chapt. 2-2. In: Glime, J. M. Bryophyte Ecology. Volume 2. 2-2-1 Bryological Interaction. Ebook sponsored by Michigan Technological University and the International Association of Bryologists. Last updated 4 July 2013 and available at <www.bryoecol.mtu.edu>. CHAPTER 2-2 PROTOZOA: CILIOPHORA AND HELIOZOA DIVERSITY TABLE OF CONTENTS Other Ciliophora Known from Bryophytes... 2-2-2 Heliozoa... 2-2-5 Summary... 2-2-5 Acknowledgments... 2-2-5 Literature Cited... 2-2-5

2-2-2 Chapter 2-2: Protozoa: Ciliophora and Heliozoa Diversity CHAPTER 2-2 PROTOZOA: CILIOPHORA AND HELIOZOA DIVERSITY Other Ciliophora Known from Bryophytes Figure 1. Amphileptus pleurosigma, a free-swimming, predatory ciliate. Photo by William Bourland. Figure 4. Cinetochilum margaritaceum stained to show organelles. Photos by William Bourland. Figure 2. Chilodontopsis depressa, an algivorous ciliate (Risse-Buhl & Küsel 2008). Photo by William Bourland. Figure 3. Cinetochilum margaritaceum, a bryophyteinhabiting ciliate that Mieczan (2007) found in peatland ponds of Poland with ph of 5.0. Photo by William Bourland. Figure 5. Didinium nasutum, a bryophyte-dwelling ciliate that feeds on Paramecium. This species is capable of encysting to avoid unfavorable conditions. Photo by William Bourland.

Chapter 2-2: Protozoa: Ciliophora and Heliozoa Diversity 2-2-3 Figure 8. Stentor showing green algal symbiont. Photo by Wim van Egmond. Figure 6. Oxytricha fallax, a ciliate, has a complex grouping of cilia that are used for sweeping food into the gullet. It lives among bryophytes, as well as other habitats. Lower organism has been stained. Photos by William Bourland. Figure 9. Colpoda steinii, a constant member of Sphagnum communities in two Polish peatlands (Mieczan 2006). Photo by Yuuji Tsukii. Figure 7. Stentor multiformis, a ciliate that occurs in peatlands (Mieczan 2006) and can attach to moss leaves. Photo by William Bourland. Figure 10. Two Holophyra species, ciliates that can inhabit Sphagnum in peatlands (Mieczan 2006). Photos by Yuuji Tsukii.

2-2-4 Chapter 2-2: Protozoa: Ciliophora and Heliozoa Diversity Figure 11. Monodinium, a ciliate that sometimes occurs on Sphagnum in peatlands (Mieczan 2006), showing ring of cilia. Photo by Yuuji Tsukii. Figure 15. Steinia sphagnicola. Normal cell. Photo by Yuuji Tsukii. Figure 12. Monodinium dividing. Photo by Yuuji Tsukii. Figure 16. Steinia sphagnicola cell dividing. Photo by Yuuji Tsukii. Figure 13. Paramecium bursaria, a common species that can occur on Sphagnum in peatlands in Poland (Mieczan 2006). This one has algal symbionts. Photo by Yuuji Tsukii. Figure 14. Spathidium muscicola, a ciliate that can live among mosses. Photo by Yuuji Tsukii. Figure 17. Upper: Urotricha farcta. Lower: Urotricha platystoma. This genus occurs on mosses in Polish peatlands (Mieczan 2006). Photo by Yuuji Tsukii.

Chapter 2-2: Protozoa: Ciliophora and Heliozoa Diversity 2-2-5 Figure 18. Strombidium viride, a ciliate that occurs occasionally on mosses in peatlands in Poland (Mieczan 2006). Photo by Yuuji Tsukii. Heliozoa The heliozoans look like a sunburst with their sticky, wirelike pseudopods. About 20 species live among Sphagnum in pools with ph ranging 5-5.6 (Hingley 1993). The sticky pseudopods, known as axopods, are used to ensnare food such as algae and smaller protozoa, and to protect the organisms. They also facilitate a slow movement, since these organisms lack cilia or flagella. The beautiful and delicate moss dwellers include Actinophrys sol (Figure 19) and Actinosphaerium eichhorni (Figure 20-Figure 21). Figure 20. Actinosphaerium eichhorni. Photo by William Bourland. Figure 21. Actinosphaerium eichhorni. Photo by William Bourland. Figure 19. Actinophrys sol showing radiating pseudopodia. Upper: Photo by Yuuji Tsukii. Lower: Photo by William Bourland. Summary Although they are more difficult to detect, the Ciliophora are quite common among bryophytes. They are best detected by culturing, and then the many species seen in this chapter become active. Heliozoa are not common among bryophytes, and only the few species shown here are familiar ones in a bryophyte habitat. Acknowledgments This chapter would not have existed without my new, but never seen, friends, William Bourland and Yuuji Tsukii. William Bourland provided me with a set of his pictures of bryophyte inhabitants. Yuuji Tsukii gave me unlimited permission to use his many, many images on the Protist Information Server website. Literature Cited Hingley, M. 1993. Microscopic Life in Sphagnum. Illustrated by Hayward, P. and Herrett, D. Naturalists' Handbook 20. [iiv]. Richmond Publishing Co. Ltd., Slough, England, 64 pp. 58 fig. 8 pl. (unpaginated).

2-2-6 Chapter 2-2: Protozoa: Ciliophora and Heliozoa Diversity Mieczan T. 2006. Species diversity of protozoa (Rhizopoda, Ciliata) on mosses of Sphagnum genus in restoration areas of the Poleski National Park. Acta Agrophys. 7: 453-459. Mieczan, T. 2007. Planktonic ciliates in peat ponds of different acidity (E Poland). Electronic Journal of Polish Agricultural Universities (EJPAU) 10 #20 accessed online at <http://www.ejpau.media.pl/volume10/issue4/art-20.html>. Risse-Buhl, U. and Küsel, K. 2008. Colonization dynamics of biofilm-associated ciliate morphotypes at different flow velocities. Eur. J. Protistol. Accessed 12 January 2009 at <http://www.sciencedirect.com/science?_ob=articleurl&_ udi=b7gw3-4twswjk- 2&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&vie w=c&_acct=c000050221&_version=1&_urlversion=0&_us erid=10&md5=72e1521f8b2c0570cf1ccbe243e4b04b>.