Mobile: ++43 (0)660 5589966 Schöcklblickstrasse 8a, A-8062 Kumberg, Austria angelikafalsone@gmail.com Personal Statements Date of birth 29.04.1976 Place of birth Leoben Citizenship Austria Marital status married, 2 children Academic Education 1994-2000 Pharmaceutical studies, Karl-Franzens-University Graz Degree awarded: Master of Pharmaceutics (Magister der Pharmazie) Diploma thesis in Pharmaceutical Chemistry at the Department of Pharm. Chemistry and Pharm. Technology, University of Graz, in the group of Prof. Dr. Andreas J. Kungl Title of the thesis: Identifikation Neuer Biologischer Bestandteile In Der Propolis (Identification Of New Biological Components In Propolis) 2000-2003 PhD thesis at the Department of Pharm. Chemistry and Pharm. Technology, University of Graz, in the group of Prof. Dr. Andreas J. Kungl Title of the thesis: The Biophysics Of Functionally Diverse Glycosaminoglycan Binding Proteins Degree awarded: Doctor rerum naturalium (Dr.rer.nat.) Topics of the PhD thesis: Cloning, expression and purification of recombinant proteins Affinity studies using fluorescence and circular dichroism spectroscopy 1 3
Studying gene regulation and protein expression in human cell cultures during inflammation Acquired expertise: Molecular principles of human inflammatory processes & angiogenesis State-of-the-art biochemical, molecular biological and cell biological techniques Biophysical techniques to investigate in vitro protein- ligand interactions and protein folding with particular respect to fluorescence spectroscopy Post graduate career Academics Jan. - May 2004 Postdoctoral fellowship in the group of Prof. Alan Fersht, MRC Cambridge, UK. Topic: Influence of glycosaminoglycans on chemokine kinetics Acquired expertise: Kinetic techniques for protein folding May 2004-Dec.2004 Postdoc. fellowship in the group of Prof. Andreas J. Kungl Topic: Proteomics of GAG binding proteins Jan. 2005-June 2007 Project leader of the Austrian Science Fund (FWF) research project ( The Kinetic Hyperspace of Chemokines ; Projectno.:P17872-B09) as an independent scientist at the Institute of Pharm. Sciences, University Graz. Industry July 2007-June 2008 Research scientist at ProtAffin Biotechnologie AG, working in the group of protein production and protein characterisation June 2008-August 2009 Head of Protein Production at ProtAffin Sept. 2009-Jan.2011 Maternity Leave Jan.2011-Sep.2011 Head of Protein Engineering at ProtAffin (part time, 20 hours) Sep.2011-November 2012 Nov. 2012-Jan.2013 Maternity Leave Head of Protein Engineering at ProtAffin (part time, 20 hours) 2
Feb. 2013-July 2013 Project/ Grant Management (part time, 20 hours) Focus of postgraduate research: Chemokine- glycosaminoglycan (GAG) interaction in vitro and in vivo Diverse protein purification techniques for the production of recombinant chemokines in lab scale Establishing large scale protein expression and purification systems Characterisation of chemokine-gag interaction at equilibrium by fluorescence and circular dichroism spectroscopy, kinetics were mainly investigated by surface plasmon resonance chamber set up Cell based in situ assays were performed in a standard Boyden Chemokine/GAG competition assay was set up in a 96 well format using fluorescence as detection method In vivo, investigation of the chemokine/gag interaction using inflammatory models, e.g. mouse-peritonitis model, mouse ear prick model and rat ischemia reperfusion injury model Involved in the implementation of a quality system at ProtAffin to assure standardised and traceable operating procedures in the labs Acquiring expertise in group leading and collaborating with Contract Research Organisations (CROs) as well as Contract Manufacturing Organistaions (CMOs) Acquiring project/ grant management skills by controlling/ accounting Österreichische Forschungsförderungsgesellschaft (FFG) programmes. managing and controlling FFG basis programmes, accounting project relevant costs and writing reports, basics in MS project, applying for an FFG BRIDGE programme Additional qualifications July - Aug. 1995, July - Aug.1997, July - Aug. 1998 Student practise in the laboratory of Voest Alpine Stahl Donawitz 3 Teaching Oct.2000 - March 2003, 2005-2006 Laboratory assistant in courses for students of Pharmaceutics at the Department of Pharm. Chemistry and Pharm. Technology, Graz
2006+2007 Lecturing Pharm. Bio.- und Gentechnologie at the Institute of Pharm. Sciences, University Graz Research November 2005 Short stay at the CNRS, Grenoble, France; in the group of Dr. Hugues Lortat-Jacob for BIAcore measurements May and June 2006 Short stay at the DKFZ, Heidelberg; in the group of Prof.Dr.med Hermann-Josef Gröne assisting in experiments of ischemia reperfusion injury in rats Professional training May 2005 Seminar Führen in Projekten (Leading projects), University Graz November 2006 Animal (mouse, rat and rabbit) handling course (Einführung in die Labortierkunde I; corresponding a FELASA category B course) at the Institute of Veterinary Medicine, Vienna June 2009 GLP and QM basis course; PromoCell Academy, Heidelberg Since October 2013: Project Management Course, Wifi Stmk List of publications: (Listed according to the date of publication) 1. Falsone A, Wabitsch V, Geretti E, Potzinger H, Gerlza T, Robinson J, Adage T, Teixeira MM, Kungl AJ (2013) Designing CXCL8-based decoy proteins with strong antiinflammatory activity in vivo. Biosci Rep. 33 (5): 743-754. 2. Adage T, Piccinini AM, Falsone A, Trinker M, Robinson J, Gesslbauer B, Kungl AJ (2012) Structure-based design of decoy chemokines as a way to explore the pharmacological potential of glycosaminoglycans. Br J Pharmacol.167(6):1195-205. 3. Piccinini AM, Knebl K, Rek A, Wildner G, Diedrichs-Möhring M, Kungl AJ. (2010) Rationally evolving MCP-1/CCL2 into a decoy protein with potent anti-inflammatory activity in vivo. J Biol Chem. 285 (12): 8782-8792. 4. Bedke J, Nelson PJ, Kiss E, Muenchmeier N, Rek A, Behnes CL, Gretz N, Kungl AJ, Gröne HJ. (2010) A novel CXCL8 protein-based antagonist in acute experimental renal allograft damage. Mol Immunol. 47 (5): 1047-1057. 4
5. Falsone SF, Kungl AJ, Rek A, Cappai R, Zangger K. (2009) The molecular chaperone Hsp90 modulates intermediate steps of amyloid assembly of the Parkinsonrelated protein alpha-synuclein. J Biol Chem. 284 (45): 31190-31199. 6. Fritzer A, Noiges B, Schweiger D, Rek A, Kungl AJ, von Gabain A, Nagy E, Meinke AL. (2009) Chemokine degradation by the Group A streptococcal serine proteinase ScpC can be reconstituted in vitro and requires two separate domains. Biochem J. 422 (3): 533-542. 7. Segerer S, Johnson Z, Rek A, Baltus T, von Hundelshausen P, Kungl AJ, Proudfoot AE, Weber C, Nelson PJ. (2009) The basic residue cluster (55)KKWVR(59) in CCL5 is required for in vivo biologic function. Mol Immunol. 46 (13): 2533-2538. 8. Rek A, Brandner B, Geretti E, Kungl AJ. (2009) A biophysical insight into the RANTES-glycosaminoglycan interaction. Biochim Biophys Acta.1794 (4): 577-582. 9. Brandner B, Rek A, Diedrichs-Möhring M, Wildner G, Kungl AJ. (2009) Engineering the glycosaminoglycan-binding affinity, kinetics and oligomerization behavior of RANTES: a tool for generating chemokine-based glycosaminoglycan antagonists. Protein Eng Des Sel. 22 (6): 367-373. 10. Rek A, Krenn E, Kungl AJ. (2009) Therapeutically targeting protein-glycan interactions. Br J Pharmacol. 157 (5): 686-694. 11. Wille I, Rek A, Krenn E, Kungl AJ. (2007) Biophysical investigation of human heparan sulfate D-glucosaminyl 3-O-sulfotransferase-3A: a mutual effect of enzyme oligomerisation and glycosaminoglycan ligand binding. Biochim Biophys Acta. 1774 (11): 1470-1476. 12. Gesslbauer B, Rek A, Falsone F, Rajkovic E, Kungl AJ. (2007) Proteoglycanomics: tools to unravel the biological function of glycosaminoglycans. Proteomics. 7(16):2870-2880. 5 13. Falsone SF, Gesslbauer B, Rek A, Kungl AJ. (2007) A proteomic approach towards the Hsp90-dependent ubiquitinylated proteome. Proteomics. 7 (14): 2375-2383. 14. Rek A, Thompson J, Roberts IS, Kungl, A.J. (2007) Biophysical investigation of recombinant K5 lyase: Structural implications of substrate binding and processing. Biochim Biophys Acta. 1774 (1): 72-77.
15. Halden Y*, Rek A*, Atzenhofer W, Szilak L, Wabnig A, Kungl AJ. (2004) Interleukin-8 binds to syndecan-2 on human endothelial cells. Biochem. J. 377, 533-538. (*Both authors contributed equally to this work) 16. Rek A, Geretti E, Goger B, Kungl AJ. (2002) The Biophysics Of Chemokine/ Glycosaminoglycan Interactions. Recent Res. Devel. Biophys. Biochem. 2, 319-340. 17. Potzinger H, Geretti E, Brandner B, Wabitsch V, Piccinini AM, Rek A, Kungl AJ. (2006) Developing chemokine mutants with improved proteoglycan affinity and knocked-out GPCR activity as anti-inflammatory recombinant drugs. Biochem. Soc. Trans. 34 (Pt 3), 435-437. 18. Rajkovic E, Rek A, Krieger E, Kungl AJ. (2004) Molecular insight into human heparanase and tumour progression. Gene Ther. Mol. Biol. 8, 523-538. 19. Goger B, Halden Y, Rek A, Mösl R, Pye D, Gallagher J, Kungl AJ. (2002) Different affinities of glycosaminoglycan oligosaccharides for monomeric and dimeric interleukin-8: a model for chemokine regulation at inflammatory sites. Biochemistry 41, 1640-1646. 20. Rek A, Falsone SF, Proudfoot AEI, Kungl AJ. Thermal unfolding of RANTES and of the structurally related chemokines MIP-1α, MCP-1 and I-TAC. Submitted to FEBS Lett. Proceeding contributions 1. Rek A, Kungl AJ. (2006) Towards an understanding of the chemokine inflammatory signal complex. Gordon Research Conference on chemotactic cytokines, Aussois, France. 2. Rek A, Falsone SF, Kungl AJ. (2005) Equilibrium unfolding of chemokines. ÖGBM Joint Meeting 2005, Vienna, P56 Session II. 6 3. Rek A, Wagner U, Thompson J, Gallagher JT, Kungl AJ. (2003) The K5 Lyase from Coliphage K5A: New Insights into the Biophysics of Phage-Born GAG Degrading Enzymes and Preliminary Crystallisation Results. Proceedings of the 5th International Conference on Molecular Structural Biology, A. J. Kungl & P. J. Kungl (Eds.), The Austrian Chemical Society (Vienna): 141
4. Rek A, Geretti E, Goger B, Gallagher JT, Proudfoot AE, Kungl AJ. (2002) The Biophysics of the RANTES/GAG interaction. Proceedings of the Chemokine Conference 2002, Madrid, Spain. 5. Goger B, Halden Y, Krieger E, Rek A, Gallagher J, Kungl AJ. (2001) Interleukin-8 dimerisation and glycosaminoglycan oligosaccharide binding. Proceedings of the 4. European Symposium of the Protein Society, Paris, France; Prot. Sci 10 (Suppl 1): A15. 6. Falsone SF, Weichel M, Rek A, Crameri R, Breitenbach M, Kungl AJ. (2001) Unfolding of the Eukaryotic Cold Shock Protein, Cla h 8, from Cladosporium Herbarum. Proceedings of the 4th International Conference on Molecular Structural Biology, A. J. Kungl & P. J. Kungl (Eds.), The Austrian Chemical Society (Vienna): 132. 7. Halden Y, Krieger E, Strasser G, Wicher U, Rek A, Andrew PJ, Kungl AJ. (2000) Interleukin-8 binding to glycosaminoglycans: a biophysical study. Biophys. J. (Suppl.) 78: 146A. Proceedings of the 44th Annual Meeting of the Biophysical Society, New Orleans, USA. 8. Kungl AJ, Falsone F, Halden Y, Kristl S, Rek A, Goger B. (2000) Funktionelle Biophysik und Proteomics. Proceedings of the 9. Österreichische Chemietage, Innsbruck, Austria. 7