1 Ph.D. student Course Molecular Neurobiology 27 May 4 June 2015 Organizers: Joost Verhaagen Netherlands Institute for Neurosciences Meibergdreef 47 1105 BA Amsterdam telephone : (020) 566 5513 fax : (020) 696 1006 e-mail : j.verhaagen@nin.knaw.nl Guus Smit Dept. of Molecular and Cellular Neurobiology VU University Faculty of Earth and Life Sciences De Boelelaan 1087 1081 HV Amsterdam telephone : (020) 598 7121 fax : (020) 598 7112 e-mail : guus.smit@cncr.vu.nl Graduate School Neurosciences Amsterdam Rotterdam
2 List of speakers Netherlands Institute for Neurosciences Koen Bossers k.bossers@nin.knaw.nl 5665512 Meibergdreef 47 Joost Verhaagen j.verhaagen@nin.knaw.nl 5665513 1105 BA Amsterdam Christiaan Levelt c.levelt@nin.knaw.nl 5665359 Matthew Mason m.mason@nin.knaw.nl 5665500 Christian Lohmann c.lohmann@nin.knaw.nl 5665500 Callan Attwell c.attwell@nin.knaw.nl 5665511 Inge Huitinga i.huitinga@nin.knaw.nl 5665521 Elizabeth Moloney e.moloney@nin.knaw.nl 5665512 VU University, Dept. of Neurosciences Ronald van Kesteren ronald.van.kesteren@vu.nl 5981161 Faculty of Earth and Life Sciences Marjo van der Knaap ms.vanderknaap@vumc.nl 4444856 De Boelelaan 1087 Guus Smit guus.smit@vu.nl 5987121 1081 HV Amsterdam Ka Wan Li k.w.li@vu.nl 5987107 Sabine Spijker s.spijker@vu.nl 5987111 Pim van Nierop p.van.nierop@vu.nl 5987114 Matthijs Verhage m.verhage@vu.nl 5986936 Sander Groffen sander.groffen@cncr.vu.nl 5986928 Ruud Toonen ruud.toonen@cncr.vu.nl 5986946 Danielle Posthuma danielle.posthuma@cncr.vu.nl 5982823 Vivi Heine vm.heine@vumc.nl 5986695 Huib Mansvelder huibert.mansvelder@cncr.vu.nl 5987097 University of Amsterdam, UVA Marten Smidt m.p.smidt@uva.nl 5256479 Postbus 94232 Arthur Bergen aabergen@amc.uva.nl 5664592 1090 GE Amsterdam Marian Weterman m.a.weterman@amc.uva.nl 5665998 Rudolf Magnus Institute for Neurosciences Giuliano Giuliani g.giuliani@umcutrecht.nl 088-7550179 P.O. Box 80040 Elly Hol E.Hol-2@umcutrecht.nl 088-7550181 3508 TA Utrecht Jeroen Pasterkamp r.j.pasterkamp@umcutrecht.nl 088-7568831 Center for Society and Genetics Martina Cornel mc.cornel@vumc.nl 020-4448910 Clinical Genetics, VUmc Van der Boechorststraat 7 1081 BT Amsterdam Netherlands Cancer Institute Titia Sixma t.sixma@nki.nl 020-5121959 Plesmanlaan 121 1066 CX Amsterdam Leiden University Medical Center Irina Pulyakhina I.Pulyakhina@lumc.nl 2300 RC Leiden Ingrid Verhaart I.E.C.Verhaart@lumc.nl 071-5269436 Annemiek Aartsma-Rus 071-5269436 International Guest Speakers: Swammerdam Lecture Daniel Geschwind
3 Locations: Lectures at the VU University: buildings and rooms are indicated in the program. Explanation locations: WN = Wis- en Natuurkunde = science building VUA, De Boelelaan 1085, Amsterdam For route description, please see: http://www.vu.nl/en/about-vu-amsterdam/contact-info-androute/route-description/index.asp All lectures in the Netherlands Institute for Neuroscience are in the Colloquium Room at the third floor of the Institute at Meibergdreef 47, Amsterdam-Zuidoost. For route description, please see: http://www.nin.knaw.nl/information/route/
4 Program Ph.D. course molecular neurobiology Wednesday 27 May 2015 (VU) *WN = Science Building VU De Boelelaan 1085 09.00-09.30 Welcome and introductory remarks on Guus Smit *WN F123 the aim of the program Basics of molecular neuroscience: the gene and gene regulation 09.30-10.15 Overview of molecular neuroscience Guus Smit * WN F123 Fundamental aspects concerning the structure of DNA and RNA, the basic organization of a gene, the process of transcription & translation. 10.15-10.30 Coffee 10.30-11.15 Manipulation of genes/dna: vectors and their applications Pim van Nierop * WN F123 DNA is hosted in vectors. Various types of vectors are available to researchers and their applications will be discussed. 11.15-12.00 The polymerase chain reaction and its applications Pim van Nierop * WN F123 12.00-13.00 Lunch Basics of molecular neuroscience: the genome, what did we learn during the last 10 years? 13.00-15.00 The structure and evolution of the genome: SNPs and CNVs Sander Groffen * WN F123 15.00-15.30 Tea 15.30-16.30 Non-coding RNA s: versatile regulators of gene expression Giuliano Giuliani * WN F123
5 Thursday 28 May 2015 (VU) *WN = Science buildingvu De Boelelaan 1085 Analysis of DNA and protein protein interactions Sequence analysis 09.00-10.30 Practical: Introduction to electronic gene databases: what is in it and how to use them? Pim van Nierop *WN C147 10.30-11.15 Protein interactions: Yeast-two-hybrid system versus mass spectrometry Guus Smit *WN C147 11.15-12.00 Protein crystallography: application in neurobiology Titia Sixma *WN C147 12.00-13.30 Lunch Proteomics 13.30-15.00 Introduction to proteomics Ka Wan Li *WN C147 15.00-15.15 Tea 15.15-16.00 Application: Mouse models of disease; proteomics analysis of the synapse. Ka Wan Li *WN C147 16.00-17.00 Demonstration: mass spectrometry Ka Wan Li *WN C147
6 Friday 29 May 2015 (NIN) High-throughput gene expression analysis 09.00-09.45 Introduction to micro-array technology Koen Bossers 09.45-10.45 Application of microarray technology: global assessment of changes in gene expression following peripheral nerve injury Ronald van Kesteren 10.45-11.00 Coffee 11.00-11.45 Ronald van Kesteren 11.45-13.00 Lunch 13.00-14.00 Bioinformatics analysis of micro-array datasets: application on human brain expression data sets Koen Bossers 14.00-15.00 Gene expression profiling using high-throughput RNAsequencing - measuring all there is?!. Irina Pulyakhina 15.00-15.30 Tea 15.30-17.00 Demonstration: microarray analysis Koen Bossers/ Matthew Mason
7 Monday June 1 2015 (VU) *WN = Science buildingvu De Boelelaan 1085 High content, medium throughput cellular screening technology 09.00-9.45 Cellomics technology: neuronal targets in peripheral nerve injury. Callan Attwell * WN F607 9.45-10.45 High-content cellular screening as a discovery tool in neuroscience Ronald van Kesteren * WN F607 10.45-11.00 Coffee 11.00-11.45 ips Center: A stem cell culture and phenotype screening facility Vivi Heine at the VU/VUMC *WN F607 11.45-13.00 Demonstration of KineticScan and Opera Reader Ronald van Kesteren and cellular phenotype screening facility *WN F607 13.00-13.30 Lunch Live cellular imaging of tagged proteins 13.30-14.30 Live imaging of GFP-tagged proteins in neurons Ruud Toonen *WN F607 14.30-15.30 Imaging structural plasticity and synaptic signaling in intact neuronal circuits Christian Lohmann * WN F607 Swammerdam Lecture Chairman: Niels Cornelisse 16.00 Daniel Geschwind *WN Q112 Department of Neurology, Psychiatry and Human Genetics UCLA Los Angeles, CA USA Integrative genomics in Autism Spectrum Disorders Followed by drinks
8 Tuesday 2 June 2015 (NIN) Gene delivery to the nervous system: in-vivo approaches 09.00-10.00 Transgenic animal technology Christiaan Levelt 10.00-11.00 Knock-out technology Sander Groffen 11.00 11.15 Coffee 11.00-12.00 Demonstration: Cold Spring Harbor video of mouse Christiaan Levelt oocyte injections, ES-cell technology, uterus implantation 12.00-13.00 Lunch 13.00-14.00 Viral vector technologie and gene therapy Joost Verhaagen 14.00-15.00 Antisense-mediated exon skipping: The example of Ingrid Verhaart/Annemiek Duchenne Muscular Dystrophy Aarstma-Rus 15.00-15.30 Tea 15.30-17.00 Random mutagenesis, forward genetics and high throughput Matthijs Verhage phenotyping in mice
9 Wednesday 3 June 2015 (NIN) Application of molecular biology to study basic neurobiological problems 09.00-10.00 Axon guidance molecules: signaling and circuit formation Jeroen Pasterkamp 10.00-11.00 Dopaminergic neurons: transcriptional determinants Marten Smidt 11.00-11.15 Coffee 11.15-12.15 Axon guidance molecules and disease: the example of RGMA Elizabeth Moloney 12.15-13.30 Lunch 13.30-14.30 Optogenetics Huib Mansvelder 14.30-15.30 Dissecting neuropsychiatric disorders: the molecular approach Sabine Spijker 15.30-16.00 Tea 16.00-17.00 Society and Genomics Martina Cornel
10 Thursday 4 June 2015 (NIN) Application of molecular biology to study neurological diseases 09.00-09.45 The Netherlands Brain Bank: high quality human brain tissue to study the molecular neuropathology of brain diseases Inge Huitinga 09.45-10.30 The molecular biology of Alzheimer's disease Elly Hol 10.30-11.00 Coffee 11.00-12.00 The genetics and molecular biology of retinal diseases Arthur Bergen 12.00-12.45 Lunch 12.45-13.45 Finding the genetic cause of a neurological disease: Marjan A. Weterman a fishy business? 13.45-14.45 Vanishing white matter diseases Marjo van der Knaap 14.45-15.00 Tea 15.00-16.00 Genome Wide Association studies (GWAS) to decipher Danielle Posthuma Complex brain disorders Drinks - NIN