Product and Pricelist valid to December 2008
Introduction This catalogue contains test kits and components for the rapid detection of biologial agents such as toxins, micro organisms and viruses. The testkits are based on the ABICAP technology (ABICAP = Antibody Binding Immune Column for Analytical Purpose). ABICAP is a flow through immuno assay system for rapid and ultra sensitive detection. It is based on the 3D-immunofiltration 1 and PolyHRP-technology 2 and allows to combination of analyte extraction and direct quantitative read out on the test columns. The system is well suited for decentralised routine testing. To this end ready to use test kits, equipment (workstation in 96 hole format) and a handheld photometer are offered. In addition, for research studies and customer specific development, assay components and reagents are available and development services are offered. 1 see booklet: "introduction to 3D Immunofiltration technology" 2 see booklet: "introduction to polyhrp technology"
Index Index ABICAP classic Test Kits Microorganisms... Viruses... Toxins... ABICAP multilayer Test Kits Microorganisms... Viruses... Toxins... Auxiliary reagents and components for ABICAP Test Kits Positive controls lyophilized pellets... Special buffers and blocker... Sample preparation tools... Equipment ABICAP Photometer... Workstations... Antibodies Microorganisms... Viruses... Toxins... Antigens Microbiological agents... Toxins... Assay components and tools Microcolumns... 10 Buffer... 10 PolyHRP Substrate... 11 PolyHRP Reagents... 11 Specific HRP- and PolyHRP Conjugates... 11 Services ABICAP custom development... 12 PolyHRP custom conjugation... 12 2 2 2 3 3 3 4 5 5 6 6 7 8 8 9 9 From a order value of 5000,00 or higher, shipping costs are included. Terms and conditions could be found on our website www.diavita.de.
ABICAP Test Kits ABICAP classic Test Kits The ABICAP classic Test kit contains 24 ABICAP columns with one specific detection filter and all reagents and instructions needed to perform quantitative assays. negative weak positive strong positive 2 Microorganisms 101 00 002 CAP Francisella tularensis ABICAP Classic 480,00 101 00 003 CAP Yersinia pestis F1 ABICAP Classic 480,00 101 00 006 CAP B. anthracis spores ABICAP Classic 480,00 101 00 007 CAP B. anthracis protective antigen ABICAP Classic 480,00 Viruses 101 00 005 CAP Ebola virus ABICAP Classic 480,00 101 00 011 CAP Flaviviruses ABICAP Classic 480,00 101 00 012 CAP Venezuela Equine Encephalitis (VEE) ABICAP Classic (under development) 101 00 019 CAP Eastern Equine Encephalitis (EEE) ABICAP Classic (under development) Toxins 101 00 008 CAP Botulinus toxin A ABICAP Classic 101 00 013 CAP Botulinus toxin B ABICAP Classic 480,00 101 00 016 CAP Botulinus toxin E ABICAP Classic 480,00 101 00 017 CAP Staphylococcus Enterotoxin B (SEB) ABICAP Classic 480,00 101 00 018 CAP Ricin ABICAP Classic 480,00
ABICAP multilayer Test Kits The ABICAP multilayer test kit contains 24 ABICAP columns in a 3 filter arrangement and all reagents to perform quantitative tests. negative weak positive strong positive Negative control Filter (indicates non specific binding) 3 Specific Measurement filter Positive control Filter (indicates function of assay reagents Microorganisms 101 00 002 CAP Francisella tularensis ABICAP multilayer 720,00 101 00 003 CAP Yersinia pestis ABICAP multilayer 720,00 Toxins 101 00 008 CAP Botulinus toxin A ABICAP multilayer 720,00 101 00 013 CAP Botulinus toxin B ABICAP multilayer 720,00 101 00 016 CAP Botulinus toxin E ABICAP multilayer 720,00 101 00 017 CAP Staphylococcus Enterotoxin B (SEB) ABICAP multilayer 720,00 101 00 018 CAP Ricin ABICAP multilayer 720,00
Auxiliary reagents and components for ABICAP Test Kits Auxiliary reagents and components for ABICAP Test Kits Positive contols Positive controls are lyophilised reagent pellets. They are reconstituted in destilled water. Microorganisms 104 00 019 PC Legionella pneumophila serotyp 1 2 controls (under development) 104 00 002 PC Francisella tularensis 2 controls 12,00 104 00 003 PC F1 control (Yersinia pestis) 2 controls 12,00 104 00 006 PC B. anthracis spores 2 controls 14,00 104 00 007 PC B. anthracis protective antigen 2 controls 14,00 4 Viruses 104 00 005 PC Ebola control 2 controls 12,00 104 00 011 PC Flaviviruses 2 controls (under development) 104 00 012 PC Venezuela Equine Encephalitis (VEE) 2 controls (under development) 104 00 019 PC Eastern Equine Encephalitis (EEE) 2 controls (under development) Toxins 104 00 008 PC Botulinus- toxin A (toxoid) 2 controls 12,00 104 00 013 PC Botulinus- toxoid B (toxoid) 2 controls 12,00 104 00 016 PC Botulinus- toxoid E (toxoid) 2 controls 12,00 101 00 017 PC Staphylococcus Enterotoxin B (SEB) 2 controls 12,00 104 00 018 PC Ricin 2 controls 14,00
Auxiliary reagents and components for ABICAP Test Kits Special buffers and blocker 201 04 005 BUF HAMA - Blocker* (Human anti-mouse antibody) 104 00 002 BUF LPS Extraction buffer** (Lipopolysaccharid) 10 mg 20-90 mg 100 mg 50 ml 100 ml 25,00 20,00/10mg 175,00 15,00 25,00 *Human anti-mouse antibodies might course false positive signals. HAMA-Blocker reduces the frequency of false results by blocking a wide range of HAM antibodies regardless of the anti-mouse epitope **Lipopolysaccharid is a useful buffer to extract LPS from gram-negative cells; thougt for sample preparations in the special case of the Francisella tularensis assay Sample preparation tools 5 For the treatment of complex sample material and prefiltration we offer sample preparation tools. They have to be selected by the customers in dependence on the sample matrix. For large volume application sample funnels (figure 3,4) are offered. Productnumber Product Quantity Price in 302 00 005 CMP sorbent filter (fig. 1) 5 20,00 302 00 009 CMP 10-20µm PE ABICAP prefilter 24 24,00 302 00 010 CMP 20-60µm PE ABICAP prefilter 24 24,00 302 00 011 CMP 40-100µm PE ABICAP prefilter 24 24,00 302 00 012 CMP 80-120µm PE ABICAP prefilter 24 24,00 302 00 013 CMP filter holder for prefilter (fig. 2) 10 60,00 302 00 007 CMP sample application funnel, 10 ml (fig. 3) 6 15,00 302 00 008 CMP sample application funnel, 50 ml (fig. 4) 12 39,00 4 1 3 2
Equipment Equipment ABICAP Photometer (not included in the Test Kits) Productnumber Product Quantity Price in 301 00 001 RDR ABICAP Photometer (portable) for ABICAP Classic and ABICAP Multilayer with key pad, incl. chip card reader, power supply, smart card reader, RS232 interface, weight approx. 300g, dimension 18x 9x3cm (fig. 5) 1 840,00 Accessories: 301 00 002 RDR PC software and cable 1 179,00 Workstations (not included in the Test Kits) 6 Productnumber Product Quantity Price in 302 00 001 CMP ABICAP Rack for 96 ABICAP Microcolumns consisting of one 96-hole frame, a stackable frame and one waste basket (fig. 6) 1 39,00 302 00 002 CMP Stackable frame for ABICAP Rack, plexiglas 1 20,00 302 00 003 CMP Stackable frame for ABICAP Rack, paperboard, disposable (fig. 7) 302 00 004 CMP Waste basket for ABICAP Rack, sterilizable (fig. 8) 25 5,00 1 20,00 302 00 005 CMP 96-hole frame to hold ABICAP Microcolumns 1 4,00 6 8 5 7
Antibodies Antibodies For all antibodies listed application notes for ELISA are available. Microorganisms Productnumber Product Amount Price in /mg 210 06 003 MAB Monoclonal anti-bacillus anthracis Protective Antigen clon 249 (for coating) IgG1 210 06 004 MAB Monoclonal anti-bacillus anthracis Protective Antigen clon 352 (for conjugation) IgG1 210 06 061 MAB Monoclonal anti-bacillus anthracis Protective Antigen, biotinylated clon 352 IgG1 480,00 7 210 06 005 MAB Monoclonal anti-bacillus anthracis spores, clon 38 (for coating) IgG2a 100 to 249 mg > 250 mg 280,00 210,00 210 06 006 MAB Monoclonal anti-bacillus anthracis spores, clon 37 (for conjugation) IgG2a 100 to 249 mg > 250 mg 280,00 210,00 210 06 062 MAB Monoclonal anti-bacillus anthracis spores, clon 37, biotinylated IgG2a 620,00 210 06 022 MAB Monoclonal anti-francisella tularensis, Reacts with LPS of F. tularensis IgG3 210 06 063 MAB Monoclonal anti-francisella tularensis, biotinylated Reacts with LPS of F. tularensis IgG3 420,00 210 06 056 PAB Polyclonal anti-legionella pneumophilia, Serotyp 1, G118 310,00 210 06 063 MAB Monoclonal anti-legionella pneumophilia, Serotyp 1, 6G5 210 06 039 MAB Monoclonal anti-legionella pneumophila, Serotyp 1 Reacts with LPS membrane of Legionella pneumophila, IgG1 210 06 048 MAB Monoclonal anti-staphylococcus aureus Enterotoxin B, Specific for enterotoxins, B, no crossreactivity with C, D, E, clon 333 (for coating), IgG1
Antibodies 210 06 049 MAB Monoclonal anti-staphylococcus aureus Enterotoxin B, Specific for enterotoxins, B, no crossreactivity with C, D, E, clon 754 (for conjugation), IgG1 210 06 064 MAB Monoclonal anti-staphylococcus aureus Enterotoxin B, biotinylated Specific for enterotoxins, B, no crossreactivity with C, D, E, clon 754 (for conjugation), IgG1 210 06 051 MAB Monoclonal anti-yersinia pestis-f1 Antigen, Reacts with capsule bearing strains of Y. pestis, IgG1 210 06 065 MAB Monoclonal anti-yersinia pestis-f1 Antigen, biotinylated Reacts with capsule bearing strains of Y. pestis, IgG1 Viruses 420,00 420,00 Productnumber Product Amount Price in /mg 8 210 06 053 MAB Monoclonal anti-ebola Virus, (for coating) IgG 210 06 059 MAB Monoclonal anti-ebola Virus, (for conjugation) IgG 210 06 061 MAB Monoclonal anti-ebola Virus, IgG, biotinylated 210 06 051 PAB Polyclonal anti-venezuela Equine Encephalitis affinity purified mouse IgG 210 06 055 PAB Polyclonal anti-eastern Equine Encephalitis affinity purified mouse IgG 210 06 056 PAB Polyclonal anti-western Equine Encephalitis affinity purified mouse IgG 210 06 060 MAB Monoclonal anti-flavivirus, group specific IgG2a 210 06 066 MAB Monoclonal anti-flavivirus, group specific IgG2a, biotinylated 280,00 210,00 280,00 210,00 620,00 280,00 210,00
Antibodies Toxins Productnumber Product Amount Price in /mg 210 06 054 MAB Monoclonal anti-botulinus A clon 22G8, (for coating) IgG2a/IgM 210 06 055 MAB Monoclonal anti-botulinus B clon C3/4 (for coating) IgG1 210 06 056 MAB Monoclonal anti-botulinus E clon 21D4 (for coating) IgG1 210 06 052 PAB Polyclonal anti-botulinus A horse, F(ab) 2 fragments 210 06 058 PAB Polyclonal anti-botulinus A, biotinylated horse, F(ab) 2 fragments 210 06 053 PAB Polyclonal anti-botulinus B horse, F(ab) 2 fragments 210 06 059 PAB Polyclonal anti-botulinus B, biotinylated horse, F(ab) 2 fragments 210 06 054 PAB Polyclonal Botulinus E horse, F(ab) 2 fragments 210 06 060 PAB Polyclonal Botulinus E, biotinylated horse, F(ab) 2 fragments 100µg 100µg 100µg 210 06 061 PAB Polyconal anti-botulinus E rabbit IgG fraction 100µg 100,00 520,00 440,00 100,00 520,00 440,00 100,00 520,00 440,00 160,00 90,00 310,00 160,00 90,00 310,00 160,00 90,00 288,00 50,00 9
Antigens Antigens Microbiological Antigens Productnumber Product Amount Price in 210 07 001 AGN Bacillus anthracis Protective Antigen (PA), isolated from the vaccine strain STI-1 210 07 053 AGN Bacillus anthracis spores attenuated, strain, inactivated 100 µg 10 6 spores 10 9 spores 210 07 002 AGN Y.pestis F1 Antigen 100 µg 210 07 003 AGN F. Tularensis, LPS Antigen 100 µg 85,00 480,00 150,00 780,00 85,00 480,00 85,00 480,00 210 07 004 AGN Legionella pneumophila serogroup 1 LPS 10 Toxins Productnumber Product Amount Price in 210 07 005 AGN Staphylococcus aureus Enterotoxin B, purified 100 µg 210 07 006 AGN Ricin, Ammoniumsulphate precipitated 100 µg 65,00 85,00 480,00
Assay components and tools Assay components and tools Many reagents used in conventional immunoassay can be applied to the 3D-Immunofiltration formate. The coating procedure of the porous filters is simple and allows the manufacturing of your own individual column assays. Microcolumns Productnumber Product Quantity Price in 102 00 003 CMP Microcolumns with frits for protein coating in packages of 192 columns 1-10 packs 100-1000 packs >1000 packs 0,62/column 0,52/column Buffer 11 Productnumber Product Amount Price in 201 04 001 CAS Casein Diluent / Blocker, ready-to-use (UCDB) 500 ml 1.000 ml 2.000 ml 4.000 ml 5.000 ml 10.000 ml 20.000 ml 40.000 ml 50.000 ml 201 04 002 BUF Blocking buffer 500 ml 1000 ml 2000 ml > 2000 ml 201 04 003 BUF PolyHRP Conjugate Stabilizer, ready-to-use 500 ml 1.000 ml 2.000 ml 4.000 ml 5.000 ml 10.000 ml 20.000 ml 40.000 ml 50.000 ml 201 04 004 BUF Substrate buffer 500 ml 1.000 ml 2.000 ml 4.000 ml 5.000 ml 10.000 ml 20.000 ml 40.000 ml 50.000 ml 150,00 270,00 500,00 800,00 1.000,00 1.600,00 2.800,00 5.000,00 6.000,00 150,00 270,00 500,00 330,00 600,00 1.100,00 1.200,00 2.200,00 3.500,00 6.000,00 7.000,00 20,00 30,00 50,00 90,00 100,00 150,00 250,00 300,00 380,00
Assay components and tools PolyHRP Substrate Productnumber Product Amount Price in 201 03 001 SUB stmb substrate solution, soluble, for ELISA-applications; ready-to-use 201 03 002 SUB ptmb substrate solution, precipitating, for 3D-IF rapid test applications (ABICAP ); ready-to-use PolyHRP Reagents 100 ml 250 ml 500 ml 1000 ml 50 ml 100 ml 250 ml 500 ml 1000 ml 30,00 80,00 135,00 235,00/L 90,00 110,00 190,00 290,00 450,00 Productnumber Product Amount Price in /mg 201 00 002 CJG SA-PolyHRP 40 2 to 9 mg 100 to 599 mg > 600 340,00 310,00 280,00 260,00 12 PolyHRP is a supermolecular enzyme complex with up to 400 molecules. The PolyHRP complex can be conjugated to various ligands and receptors. These PolyHRP-conjugates can be utilized in ELISA assays, immunoblots, immunofiltration, DNA/RNA Hybridisation. Its specific activity is by magnitudes superior compared to conventional conjugates, allowing for an substantial inhancement of assay sensitivity, optimization of assay time and reduction of reagent consumption. Specific HRP- and PolyHRP Conjugates Productnumber Product Amount Price in 201 03 001 CJG Monoclonal anti-y. Pestis-F1 - PolyHRP 40 100 µg 0,5 mg > 201 03 002 CJG Monoclonal anti-f. Tularensis - PolyHRP 40 100 µg 0,5 mg > 201 03 003 CJG Monoclonal anti-ebola Virus - PolyHRP 40 100 µg 0,5 mg 201 03 004 CJG Monoclonal anti-b.anthracis spores HRP 100 µg 0,5 mg > 300,00 1200,00 1900,00 300,00 1200,00 1900,00 380,00 1500,00 2400,00 150,00 600,00 900,00
Services Services 3D-Immunofiltration assays and the PolyHRP Technology are useful in many areas of the diagnostic and biotech industry to increase sensitivity and to reduce the assay time. Our research partner SENOVA is prepared to assist our customers in specific product development by offering: Feasibility studies Reagent development Assay development and optimisation Custom conjugation Design and development Validation and Production ABICAP custom development 13 102 01 001 SEV 3D-IF rapid test study feasibility 4.900 102 01 002 SEV 3D-IF optimisation 4.200-12.000 102 01 003 SEV 3D-IF validation 3.000-12.000 PolyHRP custom conjugation Productnumber Product Quantity/Amount Price in 201 04 001 SEV PolyHRP ELISA study (feasibility) 1 4.100 201 04 002 SEV PolyHRP - pilot conjugation up to 10 mg 4.800 201 04 003 SEV PolyHRP - conjugation This conjugation is based on the PolyHRP - pilot conjugation (201 04 002 SEV) and can only be ordered additionally 11-50 mg 51-100 mg > 100 mg 340,00 / mg 310,00 / mg / mg