Regulation of telomeres by mirnas in human cancer
|
|
- Virgil Bailey
- 8 years ago
- Views:
Transcription
1 Regulation of telomeres by mirnas in human cancer Stefan Schoeftner, PhD LNCIB, Trieste SIES Discutiamo Insieme Florence,
2 Shelterin components and telomerase are key players in human cancer Shelterin Telomerase Replicative immortality Telomere maintenance Shelterin TRF1 TRF2 POT1 TPP1 TINF2 Telomerase TERT DKC1 TERC TERRA Mouse models Progeria, Cancer Progeria, Cancer Progeria, Cancer Progeria, Cancer Progeria; Cancer Progeria; Cancer Progeria; Cancer Human Cancer Cancer Progeria Progeria; Cancer Progeria; Cancer Progeria; Cancer ICF Syndrome How is telomerase/shelterin function regulated in human cancer? Can we identify mirnas that control telomere function in human cancer?? - telo-mirnas -
3 Regulation of telomeres by mirnas in human cancer 1. mir-155 drives telomere fragility in human breast cancer Dinami et al. Cancer Research Silencing of mir-296 and mir-512 releases TERT expression in basal type breast cancer Dinami et al. Manuscript in preparation
4 luciferase reporter activity A high-throughput approach to identify mirnas with targeting specificity for telomere regulators mirna target prediction for 3 UTRs of TRF1, TRF2, POT1, TERT, DKC1 mir-vector library firefly renilla 3 UTR TRF1 transient transfection mirna/reporter HT luciferase reporter assay 2,5 2 TRF1 onco-mir-155 1,5 1,5
5 A role for TRF1 and mir-155 in human cancer TRF1, a telomere master regulator * Telomere repeat binding protein * Negative regulator of telomere length * Promoted telomere replication * Mitotic fidelity * Chromosome end protection *Telomere recombination TRF1 -/- TRF1 -/- TRF1-/- Cancer +* TRF1 -/- - Down-regulation in breast cancer - Deletion of TRF1 promotes cancer in the absence of tumorsuppression mir-155, a multipotent mirna BIC locus mir-155 mir155hg Oncomir in neoplastic disease leukemia, B-cell lymphoma breast, thyroid, colon,cervical, lung cancer Cardiovascular diseases Haematopoiesis Inflammation
6 control mir-155 control mir-155 Relative luciferase reporter activity (control set 1 ) control mir-155 antagomir-155 mir-155 targets a partially conserved sequence motif in the 3 UTR of TRF p=.1 p=.532 TRF1 actin TRF2 actin POT1 actin wt-trf1 3 UTR D mir-155 TRF1-3 UTR
7 expression (log base 2 scale) High mir-155 expression correlates with low TRF1 levels in luminal breast cancer mir-155 up-regulation in breast cancer TRF1 downregulation in breast cancer TRF1 high low TRF1 in samples with high mir-155 luminal cancer with reduced TRF1 levels 39% 28% 1% mir % 75% mir-155 +/++ tumor peritumoral tumor peri- tumor peritumoral tumoral luminal triple HER2 + 2 negative n=6 p=8.14e-5 p=.2 p=.2 mir-155 high TRF1 low High (3) Medium-low (2) Low (1) negative () 5% 25% % p=.3
8 Low TRF1 expression correlates with poor prognosis is luminal breast cancer Low TRF1 results in poor prognosis mir-155 target genes TRF1 low TRF1 high TRF1 low TRF1 high mir-155 dependent expression of TRF1 is part of a mir-155 signature that predicts poor distant metastasis free and relapse free survival in luminal breast cancer
9 control mir-155 sitrf1 mir-155 modulates telomere telomere structure and homeostasis mir-155 controls TRF1 abundance at telomeres mir-155 promotes telomere elongation p<,1 p<,1 control mir-155 telomere fluorecenece units (a.f.u.) ± 64 N=3 n= ± 88 N=3 n= ± 22 N=3 n=2213 C B A antagomir-155 +* mir-155 sitrf1 +*
10 Telomere fragility Telomere sister chromatid fusions mir-155 induces chromosomal aberrations T- loop and G-stacks: obstacles to DNA replication MCF-7 + mir-155 DNA pol 1 2 spontaneous loss of DNA sister chromatid fusions abnormally shaped telomeres multiple telomere signals telomere sister chromatid fusions
11 mir-155 drives telomere fragility in human breast cancer by targeting TRF1 mir-155 ONCO-miRNA +* mir-155 high TRF1 - low Breast Cancer proliferation/metastasis SOCS1 TP53INP1 Telomere maintenance Telomere instability (fragility, fusions) genomic instability poor prognosis Telomere maintenance Telomere +* fragility Telomere sister chromatid fusions Dinami et al. Cancer Research 214
12 normal basal luma lumb her2 normal basal luma lumb her2 expression (log base 2 scale) expression (log base 2 scale) hsa-mir-541 hsa-mir-637 hsa-mir-127-5p hsa-mir-16-1* hsa-mir-661 hsa-mir-68 hsa- hsa-mir-512-5p hsa-mir-1234 hsa-mir-491-5p hsa-mir-296-3p hsa-mir-423-5p hsa-mir-299-3p hsa-mir-532-3p hsa-mir-1323 hsa-mir-1275 hsa-mir-138 hsa-mir-76 hsa-mir-663 hsa-mir-548a-3p hsa-mir-548f hsa-mir-1249 hsa-mir-548e hsa-mir-1182 hsa-mir-1266 hsa-mir-548o hsa-mir-137 hsa-mir-632 hsa-mir-1268 luciferase reporter activity and mir-512-5p target htert and are down-regulated in breast cancer mir-vector library firefly renilla 3 UTR 4 3,5 3 2,5 2 1,5 1,5 htert - mir-512-5p - transient transfection mirna/reporter mir-512-5p
13 TERT and mir-296/mir-512 are relevant for basal type breast cancer htert signature mir-512-5p signature ALL TUMORS (DMSF) Expression low high Expression low high Expression low high htert logrank P=.49 signature logrank P=.45 mir-512-5p signature logrank P=.15 BASAL- TYPE (DMSF) Expression low high Expression low high basal Expression low high basal
14 si-con si-tert mir-con mir-512-5p Control mir-296 mir-512 mir-512 TRAP activity; controls set 1 mir si-con si-tert 1 h.i h.i h.i TSR8 mir-con mir-512-5p Control and mir-512-5p are negative regulators of telomere length and mir-512-5p target htert and reduce telomerase activity Over-expression of mir-296 or mir-512 cause telomere shortening DAPI/ Telomere Telomere p<,1 p<, p=.2 p=.3 n=9 n=9 n=9 74nt 68nt 62nt 56nt 5nt 8312 ± N=1491 ± 14.7 n=65 N=1344 n= ± N=1378 n=7 S-IC
15 MCF-7 SK-BR-3 T-47D MDA-MB-231 MDA-MB-468 MDA-MB-157 expression normalized to RNU49 cell number (x1 4 ) MCF-7 SK-BR-3 T-47D MDA-MB-231 MDA-MB-468 MDA-MB-157 mir-512-5p expression normalized to RNU49 cell number (x1 4 ) Control mir-296 mir-512 mir-512 and mir-296 reduce proliferation by driving apoptosis in MDA-MB-231 cells mir-512-5p Reduced proliferation mir-control mir-512 antago-mir-512 MDA-MB-231 *** ** mir-296/512 induce apoptosis PARP cleaved mir-control mir-296 antago-mir-296 MDA-MB-231 ** ** MDA-MB-231 Caspase cleaved actin luminal basal
16 DMSO TSA SAHA TSA+SAHA expression (untreated set 1 ) mir-512-5p expression normalized to RNU49 expression (untreated set 1 ) mir-512-5p expression (untreated set 1 ) Epigenetic silencing of mir-512 and mir-296 in basal type MDA-MB-231 cells DNMT mir-296 DNMT mir p=.1 p=.6 p= p=,7 p=.3 p= mm 5-Aza TSA SAHA p=.3 p=.3 p= p=,1 p=,8 p=, mm 5-Aza HDAC mir-296 HDAC mir TSA SAHA MDA-MB-231 (basal) DNMT MDA-MB-231 (basal) HDAC HDAC HDAC HDAC Silencing of mir-296/512 loci DNMT silent silent Silencing of mir-296/512 loci Expressed Expressed
17 htert expression normalized to actin (DMSO set 1 ) htert expression Epigenetic silencing of mir-512 and mir-296 in basal type MDA-MB-231 cells ensures htert expression HDAC X htert p=,4 p=,4 p=, TSA SAHA HDAC mirna TSA/ SAHA mir-512-5p p=.3 p=.9 con con antago / htert HDAC DNMTs mir-512-5p htert poor prognosis
18 A mirna network that underlies telomere regulation in human cancer telo-mirnas control telomeres HDAC DNMT Integrating telo-mirnas into hallmark features of cancer mir-512-5p mir-155 tumor-promotion +* a telo-mirna network to be discovered
19 Acknowledgements The Team: Roberto Dinami Rosanna Sestito Eleonora Petti Valentina Buemi Federica Cioffi Collaborators Giovanni Blandino IRE, Rome Reuven Agami, NKI Amsterdam Marcella Mottolese IRE Rome Mauro Giacca, ICGEB Trieste Maria A. Blasco CNIO, Madrid Roberta Benetti LNCIB, Trieste Claudio Schneider LNCIB, Trieste LNCIB, Area Science Park, Trieste University of Trieste
20 mir-512-5p htert luciferase reporter activity p=.2 p=.6 p=.1 p= p=.3 p= Con-miR Con-miR mir-512-5p wt- 3 UTR D UTR D UTR D83-85, UTR wt- 3 UTR D17-3 UTR
PART 3.3: MicroRNA and Cancer
BIBM 2010 Tutorial: Epigenomics and Cancer PART 3.3: MicroRNA and Cancer Dec 18, 2010 Sun Kim at Indiana University Outline of Part 3.3 Background on microrna Role of microrna in cancer MicroRNA pathway
More informationA role of microrna in the regulation of telomerase? Yuan Ming Yeh, Pei Rong Huang, and Tzu Chien V. Wang
A role of microrna in the regulation of telomerase? Yuan Ming Yeh, Pei Rong Huang, and Tzu Chien V. Wang Department of Molecular and Cellular Biology, Chang Gung University, Kwei San, Tao Yuan 333, Taiwan
More informationDepartment of BioScience Technology Chung Yuan Christian University 2015/08/13
Department of BioScience Technology Chung Yuan Christian University 2015/08/13 Cancer Cells Cancer, the 1st leading cause of death, is an example of a disease that arises from abnormalities in cell function
More informationCancer SBL101. James Gomes School of Biological Sciences Indian Institute of Technology Delhi
Cancer SBL101 James Gomes School of Biological Sciences Indian Institute of Technology Delhi All Figures in this Lecture are taken from 1. Molecular biology of the cell / Bruce Alberts et al., 5th ed.
More informationLESSON 3.5 WORKBOOK. How do cancer cells evolve? Workbook Lesson 3.5
LESSON 3.5 WORKBOOK How do cancer cells evolve? In this unit we have learned how normal cells can be transformed so that they stop behaving as part of a tissue community and become unresponsive to regulation.
More informationWhat is Cancer? Cancer is a genetic disease: Cancer typically involves a change in gene expression/function:
Cancer is a genetic disease: Inherited cancer Sporadic cancer What is Cancer? Cancer typically involves a change in gene expression/function: Qualitative change Quantitative change Any cancer causing genetic
More informationCONTRACTING ORGANIZATION: University of Alabama at Birmingham Birmingham, AL 35294
AD Award Number: W81XWH-08-1-0030 TITLE: Regulation of Prostate Cancer Bone Metastasis by DKK1 PRINCIPAL INVESTIGATOR: Gregory A. Clines, M.D., Ph.D. CONTRACTING ORGANIZATION: University of Alabama at
More informationSpecial report. Chronic Lymphocytic Leukemia (CLL) Genomic Biology 3020 April 20, 2006
Special report Chronic Lymphocytic Leukemia (CLL) Genomic Biology 3020 April 20, 2006 Gene And Protein The gene that causes the mutation is CCND1 and the protein NP_444284 The mutation deals with the cell
More informationmicrornas Non protein coding, endogenous RNAs of 21-22nt length Evolutionarily conserved
microrna 2 micrornas Non protein coding, endogenous RNAs of 21-22nt length Evolutionarily conserved Regulate gene expression by binding complementary regions at 3 regions of target mrnas Act as negative
More informationRelative Quantification of mirna Target mrnas by Real-Time qpcr. 1 Introduction. Gene Expression Application Note No. 4
Gene Expression Application Note No. 4 Relative Quantification of mirna Target mrnas by Real-Time qpcr Ute Ernst, Jitao David Zhang, Anja Irsigler, Stefan Wiemann, Ulrich Tschulena Division: Molecular
More informationLightSwitch Luciferase Assay System
Constructs, Assays & Services Assay System Promoter GoClones Synthetic Response Elements Pathway Cell Lines 3 UTR GoClones mirna Mimics & Inhibitors Synthetic mirna Targets Transfection Reagents Assay
More informationClonetics Conditionally Immortalized Human Cells. Relevant Cells for High Throughput Screening
Clonetics Conditionally Immortalized Human Cells Relevant Cells for High Throughput Screening Clonetics Conditionally Immortalized Cell Strains Conditionally immortalized cell strains are primary cells
More informationGlobal microrna level regulation of EGFR-driven cell cycle protein network in breast cancer
Molecular Systems Biology Peer Review Process File Global microrna level regulation of EGFR-driven cell cycle protein network in breast cancer Stefan Uhlmann, Heiko Mannsperger, Jitao David Zhang, Emőke-Ágnes
More informationIntroduction. Cancer Biology. Tumor-suppressor genes. Proto-oncogenes. DNA stability genes. Mechanisms of carcinogenesis.
Cancer Biology Chapter 18 Eric J. Hall., Amato Giaccia, Radiobiology for the Radiologist Introduction Tissue homeostasis depends on the regulated cell division and self-elimination (programmed cell death)
More informationCancer: Cells Behaving Badly
Cancer: Cells behaving badly It s quite likely your body is harboring precancerous cells. Don t panic: this doesn t mean you have cancer. Many of us have cells here and there with defense mechanisms that
More informationTranslating DNA repair pathways into therapeutic targets: beyond the BRCA1/2 and PARP inhibitor saga. Jorge S Reis-Filho, MD PhD FRCPath
Translating DNA repair pathways into therapeutic targets: beyond the BRCA1/2 and PARP inhibitor saga Jorge S Reis-Filho, MD PhD FRCPath Summary How do PARP inhibitors work? Synthetic lethality Potential
More informationOriGene Technologies, Inc. MicroRNA analysis: Detection, Perturbation, and Target Validation
OriGene Technologies, Inc. MicroRNA analysis: Detection, Perturbation, and Target Validation -Optimal strategies to a successful mirna research project Optimal strategies to a successful mirna research
More informationSUPER SENSITIVE TM. mirna in FFPE. Next-Gen in situ Tissue Signature Markers Potential tool for Characterization of CUP
SUPER SENSITIVE TM mirn in FFPE Next-Gen in situ Tissue Signature Markers Potential tool for haracterization of UP mirn in FFPE mirn Processing MicroRNs (mirns) are endogenous, non-coding RNs known to
More information岑 祥 股 份 有 限 公 司 技 術 專 員 費 軫 尹 20100803
技 術 專 員 費 軫 尹 20100803 Overview of presentation Basic Biology of RNA interference Application of sirna for gene function? How to study mirna? How to deliver sirna and mirna? New prospects on RNAi research
More informationAppendix C DNA Replication & Mitosis
K.Muma Bio 6 Appendix C DNA Replication & Mitosis Study Objectives: Appendix C: DNA replication and Mitosis 1. Describe the structure of DNA and where it is found. 2. Explain complimentary base pairing:
More informationPREPARED FOR: U.S. Army Medical Research and Materiel CommandFort Detrick, Maryland 21702 5012
AD Award Number: W81XWH 07 1 0542 TITLE: Is Nuclear Structure Altered in Breast Cancer Cells? PRINCIPAL INVESTIGATOR: Han Htun, Ph.D. CONTRACTING ORGANIZATION: University of California Los Angeles, CA
More informationDiscovery & Modeling of Genomic Regulatory Networks with Big Data
Discovery & Modeling of Genomic Regulatory Networks with Big Data Hamid Bolouri Division of Human Biology Fred Hutchinson Cancer Research Center labs.fhcrc.org/bolouri I have no financial relationships
More informationmirnaselect pegp-mir Cloning and Expression Vector
Product Data Sheet mirnaselect pegp-mir Cloning and Expression Vector CATALOG NUMBER: MIR-EXP-GP-C STORAGE: -80ºC QUANTITY: 100 µl of bacterial glycerol stock Components 1. mirnaselect pegp-mir Cloning
More informationSommaire projets sélectionnés mesure 29: Soutien à la recherche translationnelle
Sommaire projets sélectionnés mesure 29: Soutien à la recherche translationnelle TITLE PROJET NOM HOPITAL Assessment of tumor angiogenesis using PET/CT with 18 F-Galacto- RGD. (PNC_29_001) Division of
More informationTelomere Stabilizing Mechanisms
Telomere Stabilizing Mechanisms Cell Immortalization and Tumorigenesis Basic Seminar Malignant Diseases I N094 PhD Program Malignant Diseases N790 Dr Program Clinical Experimental Oncology N090 Dr Program
More information4.1 3T12 and 312 are immortalized cell lines with transforming potential:
DISCUSSION CHAPTER 4 4.1 3T12 and 312 are immortalized cell lines with transforming potential: Transformation of a normal cell with finite number of divisions into a tumorigenic cell of potentially infinite
More informationLezioni Dipartimento di Oncologia Farmacologia Molecolare. RNA interference. Giovanna Damia 29 maggio 2006
Lezioni Dipartimento di Oncologia Farmacologia Molecolare RNA interference Giovanna Damia 29 maggio 2006 RNA INTERFERENCE Sequence-specific gene suppression by dsrnas Gene silencing by dsrna: C. elegans
More informationCancer Genomics: What Does It Mean for You?
Cancer Genomics: What Does It Mean for You? The Connection Between Cancer and DNA One person dies from cancer each minute in the United States. That s 1,500 deaths each day. As the population ages, this
More informationUpdate in Hematology Oncology Targeted Therapies. Mark Holguin
Update in Hematology Oncology Targeted Therapies Mark Holguin 25 years ago Why I chose oncology People How to help people with possibly the most difficult thing they may have to deal with Science Turning
More informationMicroRNA signatures in human cancers
MicroRNA signatures in human cancers George A. Calin and Carlo M. Croce Abstract MicroRNA (mirna ) alterations are involved in the initiation and progression of human cancer. The causes of the widespread
More informationNotch 1 -dependent regulation of cell fate in colorectal cancer
Notch 1 -dependent regulation of cell fate in colorectal cancer Referees: PD Dr. Tobias Dick Prof. Dr. Wilfried Roth http://d-nb.info/1057851272 CONTENTS Summary 1 Zusammenfassung 2 1 INTRODUCTION 3 1.1
More informationChapter 12: The Cell Cycle
Name Period Chapter 12: The Cell Cycle Overview: 1. What are the three key roles of cell division? State each role, and give an example. Key Role Example 2. What is meant by the cell cycle? Concept 12.1
More informationModulation of microrna editing, expression and processing by ADAR2 deaminase in glioblastoma
Tomaselli et al. Genome Biology (2015) 16:5 DOI 10.1186/s13059-014-0575-z RESEARCH Open Access Modulation of microrna editing, expression and processing by ADAR2 deaminase in glioblastoma Sara Tomaselli
More informationCancer. 9p21.3 deletion. t(12;21) t(15;17)
CANCER FISH PROBES INDIVIDUAL AND PANEL S Acute Lymphoblastic Leukemia (ALL) ALL FISH Panel (includes all probes below) 8010 LSI MYB/CEP6 LSI p16 (CDKN2A) LSI BCR/ABL with ASS LSI ETV6 (TEL)/AML1 (RUNX1)
More informationJuliana Xu Lorraine J. Gudas, Ph.D. Weill Medical College of Cornell University New York, NY 10021
AD Award Number: DAMD17-01-1-0218 TITLE: Combination Therapy Employing Retinoids and Chromatin Remodeling Agents in Human Breast Carcinomas PRINCIPAL INVESTIGATOR: Juliana Xu Lorraine J. Gudas, Ph.D. CONTRACTING
More information2006 7.012 Problem Set 6 KEY
2006 7.012 Problem Set 6 KEY ** Due before 5 PM on WEDNESDAY, November 22, 2006. ** Turn answers in to the box outside of 68-120. PLEASE WRITE YOUR ANSWERS ON THIS PRINTOUT. 1. You create an artificial
More informationTEXT PICTURE. Martin Puhr PhD. Medical University of Innsbruck. Department of Urology Division of Experimental Urology
TEXT PIAS1 is a determinant of poor survival and acts as a positive feedback regulator of AR signaling through enhanced AR stabilization in prostate cancer PICTURE Martin Puhr PhD Department of Urology
More informationNuove mutazioni nella leucemia mielomonoci/ca cronica
Nuove mutazioni nella leucemia mielomonoci/ca cronica Firenze 31 marzo 2011 DANIEL A CILLONI UNIVERSITA DEGLI STUDI DI TORINO DIPARTIMENTO DI SCIENZE CLINICHE E BIOLOGICHE c Ros1 Human Human ortholog of
More informationA powerful model of endometrial carcinogenesis! molecular analysis and rational design of immunological intervention approaches
A powerful model of endometrial carcinogenesis! molecular analysis and rational design of immunological intervention approaches Afrouz Behboudi PhD, Associate Prof. School of Life Sciences, University
More informationDal germinale al somatico nella identificazione di tumori ereditari
Modena 18-19 novembre 2010 Dal germinale al somatico nella identificazione di tumori ereditari Laura Ottini Tendencies to develop cancer can be inherited Fletcher & Houlston, 2010 Cancer is a genetic disease
More informationMicroreg organism and Small RN Cancer
micrornas and cancer Cellular and Molecular Biology of Cancer (PATH G4500-001) October 29, 2014 Katia Basso, PhD Office: ICRC RM506 E-mail: kb451@columbia.edu -Katia Basso- Columbia University From the
More informationDIPARTIMENTO di SCIENZE BIOMEDICHE e BIOTECNOLOGICHE
July 15, 2015 Science editor of World Journal of Gastroenterology Jing Yu Email: j.yu@wjgnet.com Dear Prof. Jing Yu, please find enclosed a revised version of our paper Non-coding landscapes of Colorectal
More informationNew strategies in anticancer therapy
癌 症 診 療 指 引 簡 介 及 臨 床 應 用 New strategies in anticancer therapy 中 山 醫 學 大 學 附 設 醫 院 腫 瘤 內 科 蔡 明 宏 醫 師 2014/3/29 Anti-Cancer Therapy Surgical Treatment Radiotherapy Chemotherapy Target Therapy Supportive
More informationGuided Notes: Chapter 9 Cellular Reproduction
Guided Notes: Cellular Reproduction When do cells divide? Cells grow and function normally until they become too. Cell size is because increases faster than This means that there is not enough area on
More informationMicroRNA formation. 4th International Symposium on Non-Surgical Contraceptive Methods of Pet Population Control
MicroRNA formation mirna s are processed from several precursor stages Mammalian genomes seem to have 100 s of mirna s Nucleotides in positions 2-8 of an mirna are considered the mirna seed 5 Methyl-G
More informationMicroRNA Mike needs help to degrade all the mrna transcripts! Aaron Arvey ISMB 2010
Target mrna abundance dilutes microrna and sirna activity MicroRNA Mike needs help to degrade all the mrna transcripts! Aaron Arvey ISMB 2010 Target mrna abundance dilutes microrna and sirna activity Erik
More informationCell Division Mitosis and the Cell Cycle
Cell Division Mitosis and the Cell Cycle A Chromosome and Sister Chromatids Key Points About Chromosome Structure A chromosome consists of DNA that is wrapped around proteins (histones) and condensed Each
More informationMUTATION, DNA REPAIR AND CANCER
MUTATION, DNA REPAIR AND CANCER 1 Mutation A heritable change in the genetic material Essential to the continuity of life Source of variation for natural selection New mutations are more likely to be harmful
More informationBiomedicine The background. The main interest. The tools
1 Biomedicine The background The main interest? Bioinformatics Clinical informatics The tools 2 Outline 3 Outline 4 Working on Network Data Analysis HH RR Infrastructure Training BIOCOMPUTATION & STRUCTURAL
More informationmicrorna Signatures in Cancer E. ROBERT WASSMAN, MD, FAAP, FACMG CHIEF MEDICAL OFFICER, ROSETTA GENOMICS
microrna Signatures in Cancer E. ROBERT WASSMAN, MD, FAAP, FACMG CHIEF MEDICAL OFFICER, ROSETTA GENOMICS 1st International Congress on Controversies in Personalized Oncology (CONPO) BARCELONA, SPAIN MARCH
More informationEXPRESSION ARREST shrna mir GENOME- WIDE LIBRARIES
C GUGAAG EXPRESSION ARREST shrna mir GENOME- WIDE LIBRARIES MicroRNA-adapted shrna (shrna mir ) for increased, specific and consistent knockdown. MicroRNA PROCESSING PATHWAY UTILIZED FOR shrna mir Developed
More informationChromosomes, Karyotyping, and Abnormalities (Learning Objectives) Learn the components and parts of a metaphase chromosome.
Chromosomes, Karyotyping, and Abnormalities (Learning Objectives) Learn the components and parts of a metaphase chromosome. Define the terms karyotype, autosomal and sex chromosomes. Explain how many of
More informationCCR Biology - Chapter 5 Practice Test - Summer 2012
Name: Class: Date: CCR Biology - Chapter 5 Practice Test - Summer 2012 Multiple Choice Identify the choice that best completes the statement or answers the question. 1. If a cell cannot move enough material
More information# Circuits relevant to cancer, related to known TFs.
# Circuits relevant to cancer, related to known TFs. # Final dataset of circuits where at least one gene has been annotated as relevant to cancer. # Circuits correspond to blocks separated by a symbol.
More informationBioMmune Technologies Inc. Corporate Presentation 2015
BioMmune Technologies Inc Corporate Presentation 2015 * Harnessing the body s own immune system to fight cancer & other autoimmune diseases BioMmune Technologies Inc. (IMU) ABOUT A public biopharmaceutical
More informationLEUKEMIA LYMPHOMA MYELOMA Advances in Clinical Trials
LEUKEMIA LYMPHOMA MYELOMA Advances in Clinical Trials OUR FOCUS ABOUT emerging treatments Presentation for: Judith E. Karp, MD Advancements for Acute Myelogenous Leukemia Supported by an unrestricted educational
More informationSupplementary Figure 1.
Supplementary Figure 1. (A) MicroRNA 212 enhances IS from pancreatic β-cells. INS-1 832/3 β-cells were transfected with precursors for mirnas 212, 375, or negative control oligonucleotides. 48 hrs after
More informationExploiting science for engineering: BRCA2 targeted therapies
20.109 MOD1 DNA ENGINEERING Fall 2010 Exploiting science for engineering: BRCA2 targeted therapies Orsi Kiraly Engelward lab Homologous recombination is important No HR chromosomal aberrations cell death
More informationSUPPLEMENTARY DATA 1
SUPPLEMENTARY DATA 1 Supplementary Figure S1. Overexpression of untagged Ago2 inhibits the nuclear transport of the dotted foci of GFP-signal of myc-gfp-tnrc6a-nes-mut. (A C) HeLa cells expressing myc-gfp-tnrc6a-nes-mut
More informationLecture 3: Mutations
Lecture 3: Mutations Recall that the flow of information within a cell involves the transcription of DNA to mrna and the translation of mrna to protein. Recall also, that the flow of information between
More informationChapter 12: The Cell Cycle
Name Period Chapter 12: The Cell Cycle Overview: 1. What are the three key roles of cell division? State each role, and give an example. Key Role Reproduction Growth and development Tissue removal Example
More informationLeukemia Research Foundation 2004-2005 Scientific Research Grant Recipients
Page 1 of 5 NEW INVESTIGATOR AWARDS Ioannis Aifantis, Ph.D. The University of Chicago, Chicago, IL $75,000.00 Cooperation of Notch and pre-tcr Signaling in the Induction of T Cell Leukemia The pre-t Cell
More informationThe Human Genome Project
The Human Genome Project Brief History of the Human Genome Project Physical Chromosome Maps Genetic (or Linkage) Maps DNA Markers Sequencing and Annotating Genomic DNA What Have We learned from the HGP?
More informationCURRICULUM VITAE. Tabitha M. Hardy
CURRICULUM VITAE Tabitha M. Hardy Education: Doctor of Philosophy (Ph.D.), Microbiology and Immunology, Indiana University at Indiana University-Purdue University Indianapolis, Indianapolis, IN, 2004-2010
More informationIdentification of CD4+ T cell epitopes specific for the breast cancer associated antigen NY-BR-1
9/8/2015 Identification of CD4+ T cell epitopes specific for the breast cancer associated antigen NY-BR-1 Stefan Eichmüller, PhD GMP & T Cell Therapy Unit, German Cancer Research Center, Heidelberg, Germany
More informationProgramme. Michele Grieco Dipartimento di Scienze Ambientali Seconda Università degli Studi di Napoli Naples, Italy
Programme Invited Speakers: Reuven Agami (The Netherlands), Manuela Baccarini (Austria), Allan Balmain (USA), Daniel Birnbaum (France), Anne-Lise Børresen-Dale (Norway), Carlos Caldas (UK), Silvana Canevari
More informationJanuary 23, 2006 - SCIENTIFIC PROGRAM
January 23, 2006 - SCIENTIFIC PROGRAM Session A: MOLECULAR & CELLULAR BIOLOGY PART I Afternoon session: h. 15.00/23.00 11:00-12:30 Registration and poster positioning 13:00-14:30 Welcome Lunch 14:50-15:00
More informationModelli murini di linfomagenesi. Roberto Chiarle, M.D. Firenze, 24/11/2011
Modelli murini di linfomagenesi Roberto Chiarle, M.D. Firenze, 24/11/2011 Chromosomal translocations in tumors Translocation are common in many types of tumors and are often considered primary oncogenic
More informationtreatments) worked by killing cancerous cells using chemo or radiotherapy. While these techniques can
Shristi Pandey Genomics and Medicine Winter 2011 Prof. Doug Brutlag Chronic Myeloid Leukemia: A look into how genomics is changing the way we treat Cancer. Until the late 1990s, nearly all treatment methods
More informationINSERM/ A. Bernheim. Overcoming clinical relapse in multiple myeloma by understanding and targeting the molecular causes of drug resistance
A. Bernheim Overcoming clinical relapse in multiple myeloma by understanding and targeting the molecular causes of drug resistance OVER-MyR is funded by the European Commission within its FP7 specific
More informationPathway Analysis : An Introduction
Pathway Analysis : An Introduction Experiments Literature and other KB Data Knowledge Structure in Data through statistics Structure in Knowledge through GO and other Ontologies Gain insight into Data
More informationMULTIPLE MYELOMA. Dr Malkit S Riyat. MBChB, FRCPath(UK) Consultant Haematologist
MULTIPLE MYELOMA Dr Malkit S Riyat MBChB, FRCPath(UK) Consultant Haematologist Multiple myeloma is an incurable malignancy that arises from postgerminal centre, somatically hypermutated B cells.
More informationTransfection-Transfer of non-viral genetic material into eukaryotic cells. Infection/ Transduction- Transfer of viral genetic material into cells.
Transfection Key words: Transient transfection, Stable transfection, transfection methods, vector, plasmid, origin of replication, reporter gene/ protein, cloning site, promoter and enhancer, signal peptide,
More informationVHL and clear cell Renal Cell Carcinoma. Expression profiling reveals cell of origin. Renal Cell Carcinoma (RCC) Title. VHL syndrome hallmark cancer:
VHL and clear cell Renal Cell Carcinoma Gene expression profiles in renal cell carcinoma October 23, 2014 VHL syndrome hallmark cancer: Clear cell renal cell carcinoma (ccrcc) VHL mutations also a hallmark
More informationIf and when cancer cells stop dividing, they do so at random points, not at the normal checkpoints in the cell cycle.
Cancer cells have escaped from cell cycle controls Cancer cells divide excessively and invade other tissues because they are free of the body s control mechanisms. Cancer cells do not stop dividing when
More informationChromosomes, Mapping, and the Meiosis Inheritance Connection
Chromosomes, Mapping, and the Meiosis Inheritance Connection Carl Correns 1900 Chapter 13 First suggests central role for chromosomes Rediscovery of Mendel s work Walter Sutton 1902 Chromosomal theory
More informationLesson 3 Reading Material: Oncogenes and Tumor Suppressor Genes
Lesson 3 Reading Material: Oncogenes and Tumor Suppressor Genes Becoming a cancer cell isn t easy One of the fundamental molecular characteristics of cancer is that it does not develop all at once, but
More informationOutline. Predictive Assays in Radiation Therapy Immunotherapy in Cancer Treatment. Introduction. Current clinical practice
Predictive Assays in Radiation Therapy Immunotherapy in Cancer Treatment Radiation Biology Outline Introduction: Predictive assays in radiation therapy Examples for specific tumors Immunotherapy Summary
More information1-7 2-8 3-9 4-10 5-11 6-12 7-13 8-14 9-15. Corresponding position of sirna GS
A C GS Target transcript 5ʼ- - 3ʼ 3ʼ- 21 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1-5ʼ 10-16 11-17 12-18 13-19 14-20 15-21 sivim-270 3ʼUTR 1-7 2-8 3-9 4-10 5-11 6-12 7-13 8-14 9-15 Corresponding
More informationSECOND PRIMARY BREAST CANCERS FOLLOWING HAEMATOLOGIC MALIGNANCIES A CASE SERIES STUDY FARAH TANVEER PGY 3 DR.MEIR WETZLER DR.
SECOND PRIMARY BREAST CANCERS FOLLOWING HAEMATOLOGIC MALIGNANCIES A CASE SERIES STUDY FARAH TANVEER PGY 3 DR.MEIR WETZLER DR. TRACEY O CONNOR RESEARCH QUESTON Patients with previously diagnosed hematologic
More informationTherapeutic inhibition of TRF1 impairs the growth of p53-deficient K-Ras G12V -induced lung cancer by induction of telomeric DNA damage
Research Article Therapeutic inhibition of TRF1 impairs the growth of p53-deficient K-Ras G12V -induced lung cancer by induction of telomeric DNA damage María García-Beccaria 1,, Paula Martínez 1,, Marinela
More informationBreast cancer and normal cell lines
The Essentials of Life Science Research Globally Delivered Breast cancer and normal cell lines ATCC No. Name Tissue Cell Type Disease Oganism Age Ethnicity Genes Expressed CRL-7227 Hs 281.T CRL-7245 Hs
More informationMalignant Lymphomas and Plasma Cell Myeloma
Malignant Lymphomas and Plasma Cell Myeloma Dr. Bruce F. Burns Dept. of Pathology and Lab Medicine Overview definitions - lymphoma lymphoproliferative disorder plasma cell myeloma pathogenesis - translocations
More informationPsychoonkology, Sept. 2010 lifestyle factors and epigenetics
Psychoonkology, Sept. 2010 lifestyle factors and epigenetics Alexander G. Haslberger Dep. für Ernährungswissenschaften Univ. of Vienna Working group: Food, GI-Microbiology, Epigenetics Content Health:
More informationmirnaselect pep-mir Cloning and Expression Vector
Product Data Sheet mirnaselect pep-mir Cloning and Expression Vector CATALOG NUMBER: MIR-EXP-C STORAGE: -80ºC QUANTITY: 2 vectors; each contains 100 µl of bacterial glycerol stock Components 1. mirnaselect
More informationMolekylært målrettet medicinsk kræftbehandling for klinikere principper og metoder
Molekylært målrettet medicinsk kræftbehandling for klinikere principper og metoder Professor Claus Lindbjerg Andersen Department of Molecular Medicine (MOMA) Aarhus University hospital Outline The central
More informationAnalysis and Integration of Big Data from Next-Generation Genomics, Epigenomics, and Transcriptomics
Analysis and Integration of Big Data from Next-Generation Genomics, Epigenomics, and Transcriptomics Christopher Benner, PhD Director, Integrative Genomics and Bioinformatics Core (IGC) idash Webinar,
More informationImmunologie für Studierende der Biologie & Molekularmedizin. mirna und Immunität. Jürgen Wittmann (jwittman@molmed.uni-erlangen.
Immunologie für Studierende der Biologie & Molekularmedizin Konzepte der Immunologie Erlangen SS 2008 mirna und Immunität Jürgen Wittmann (jwittman@molmed.uni-erlangen.de) Division of Molecular Immunology
More informationAppendix 2 Molecular Biology Core Curriculum. Websites and Other Resources
Appendix 2 Molecular Biology Core Curriculum Websites and Other Resources Chapter 1 - The Molecular Basis of Cancer 1. Inside Cancer http://www.insidecancer.org/ From the Dolan DNA Learning Center Cold
More informationThermo Scientific DharmaFECT Transfection Reagents
Thermo Scientific Transfection Reagents Maintain cell viability with lipids specially formulated to deliver sirna Achieve robust sirna uptake for dependable gene silencing Expand your RNAi applications
More informationQuantitative 1-Schritt-DNA-Methylierungsanalyse aus genomischer DNA
Quantitative 1-Schritt-DNA-Methylierungsanalyse aus genomischer DNA Molekulare Diagnostik 2011 Departement Klinische Forschung Abteilung für Humangenetik Experimentelle Hämatologie DKF und Labor für Molekulare
More informationMonoclonal Antibodies in Cancer. Ralph Schwall, PhD Associate Director, Translational Oncology Genentech, Inc.
Monoclonal Antibodies in Cancer Ralph Schwall, PhD Associate Director, Translational Oncology Genentech, Inc. Disclaimer I had nothing to do with Herceptin Using lessons learned in new antibody projects
More informationCD22 Antigen Is Broadly Expressed on Lung Cancer Cells and Is a Target for Antibody-Based Therapy
CD22 Antigen Is Broadly Expressed on Lung Cancer Cells and Is a Target for Antibody-Based Therapy Joseph M. Tuscano, Jason Kato, David Pearson, Chengyi Xiong, Laura Newell, Yunpeng Ma, David R. Gandara,
More information1 Mutation and Genetic Change
CHAPTER 14 1 Mutation and Genetic Change SECTION Genes in Action KEY IDEAS As you read this section, keep these questions in mind: What is the origin of genetic differences among organisms? What kinds
More informationHuman Genome Organization: An Update. Genome Organization: An Update
Human Genome Organization: An Update Genome Organization: An Update Highlights of Human Genome Project Timetable Proposed in 1990 as 3 billion dollar joint venture between DOE and NIH with 15 year completion
More informationViral Infection: Receptors
Viral Infection: Receptors Receptors: Identification of receptors has come from expressing the gene for the receptor in a cell to which a virus does not normally bind -OR- By blocking virus attachment
More informationCell Growth and Reproduction Module B, Anchor 1
Cell Growth and Reproduction Module B, Anchor 1 Key Concepts: - The larger a cell becomes, the more demands the cell places on its DNA. In addition, a larger cell is less efficient in moving nutrients
More informationMicro RNAs: potentielle Biomarker für das. Blutspenderscreening
Micro RNAs: potentielle Biomarker für das Blutspenderscreening micrornas - Background Types of RNA -Coding: messenger RNA (mrna) -Non-coding (examples): Ribosomal RNA (rrna) Transfer RNA (trna) Small nuclear
More informationSuperior TrueMAB TM monoclonal antibodies for the recognition of proteins native epitopes
Superior TrueMAB TM monoclonal antibodies for the recognition of proteins native epitopes Outlines Brief introduction of OriGene s mission on gene-centric product solution. TrueMAB monoclonal antibody
More information--- New Course Request Indiana University INDIANAPOLIS camp^ Other topics include the clinical
New Course Request Indiana University INDIANAPOLIS camp^ Check Appropriate Boxes: Undergraduate credit Graduate credit Q Professional credit - 1. School/Division Medicine / Graduate 2. Academic Subject
More information