SOLUTIONS FOR NEXT-GENERATION SEQUENCING

Transcription

1 SOLUTIONS FOR NEXT-GENERATION SEQUENCING GENOMICS CELL BIOLOGY PROTEOMICS AUTOMATION enabling next-generation research

2 From Samples To Publication, Millennium Science Enables Your Next-Gen Sequencing Workflow target capture & enrichment automated size cdna synthesis & amplification sample Millennium Science now offers a suite of high quality products specifically for your Next-Generation Sequencing workflow. YOUR NEXT- GENERATION SEQUENCER SAMPLES PUBLICATION We offer a range of products for use at select stages of the NGS workflow which are compatible across all Next Generation Sequencing platforms. 2

3 Cell Lysis and RNA Extraction The NuGEN Prelude TM Direct Lysis Module provides a fast and easy method for whole transcriptome using direct cell lysates without RNA isolation. Cell washing and lysis takes less than five minutes and the resulting lysates can be added directly to downstream NGS reactions. Prelude Direct Lysis Module Traditional RNA extraction and isolation procedures are eliminated Simple assay protocol allows for RNA amplification directly from the Prelude Direct Lysis Buffer saving time and money Can be used with as few as 50 cells or as many as 10,000 cells. Assay flexibility to cater for your throughput needs Samples can be assayed directly from cell lysate without the need for further purification or can be frozen for later use High performance : microarray and NGS expression profiling demonstrates highly concordant results of greater than 95% between cell lysate and isolated total RNA NuGEN Prelude TM Direct Lysis Module 3

4 cdna Synthesis and Amplification The Ovation TM RNA-Seq System provides a fast and simple method for preparing amplified cdna from total RNA for RNA-Seq applications while the Ovation 3 -DGE System provides accurate expression (tag profiling) suitable for quantification of any poly(a)+ transcripts, without the need for prior sequence knowledge. Ovation RNA-Seq System Allows for transcriptome of small amounts of RNA Analysis of both poly and nonpolyadenylated transcripts Double-stranded cdna product is optimized for Only 500pg of starting material is required which produces sufficient cdna for several NGS runs Assay takes only 6-hours total time No rrna reduction or poly(a) selection is required Ovation 3 -DGE System Compatible with all NGS platforms Flexible input - use as little as 10ng of total RNA or product of the Prelude Direct Lysis system from as few as 50 cells 9 hours total assay time Prior sequence knowledge is not required for expression profiling Increase power & reduce costs with the optional indexing capability of the NuGEN Encore TM NGS Library System NuGEN Ovation RNA-Seq System NuGEN Ovation 3 DGE System 4

5 Target Capture and Enrichment Fluidigm s Access Array TM System delivers the throughput and ease-of-use required to get the most out of your next generation sequencer. The Access Array is an open platform comprised of a microfluidic chip that allows amplification of 48 specific amplicons from 48 unique samples, in effect preparing 48 libraries in just a few hours. Sample Capture and Target Enrichment Only sequence areas of interest Sample Barcoding for Multiplexed Sequencing Sequence multiple samples per run Sequencing Library Preparation Using Amplicon Tagging Eliminate traditional High Throughput Enriched targets from 48 samples per chip Easy-to-Use Only six manual steps required to produce 2,304 amplicons Time efficient Prepares 48 libraries in a few hours at minimal cost Access Array AX + SATC System Access Array Access Array Reagent Kit 5

6 Automated Size Fractionation The LabChip XT from Caliper Life Sciences delivers reproducible, tightly sized samples in a sequencing buffer using microfluidics. The XT improves laboratory efficiency by performing automated nucleic acid accurately and reproducibly and provides sizing that is difficult to obtain using manual methods. LabChip XT Fast, reproducible, high resolution size selection Less than 30 minute processing Up to 4 samples processed simultaneously per chip Quantitative, digital Sample tracking via barcode Completely independent channels minimizes potential for cross contamination No post-purification, with collection in PCR-compatible buffer LabChip XT System LabChip XT DNA500 Chip & Reagent Kit 6

7 Library Construction For on the Illumina NGS System, the Encore TM NGS Library Systems from NuGEN has been developed for seamless integration with both the Ovation RNA-Seq System and Ovation 3 -DGE System to enable a complete end-to-end solution for transcriptome using total RNA samples in as little as 9 hours. Encore TM NGS Library System I & Encore TM NGS Library Multiplex System I Library in as little as three hours, with only two purification steps and no gel purification required All required components are included to make libraries for RNA- Seq, genomic DNA sequencing, ChIP-Seq or Digital Gene Expression applications on Illumina NGS platforms Optional indexing capability for multiplex sequencing to increase sample throughput and reduce sequencing costs Seamless integration with NuGEN s Ovation RNA-Seq and 3 -DGE Systems Compatible with the Illumina NGS sequencing platforms NuGEN Encore NGS Library System I NuGEN Encore NGS Library Multiplex System I 7

8 NGS Data Analysis NextGENe from SoftGenetics is unique biologist-friendly software that analyses the vast amount of generated by Next-Generation Sequencing. Its simplicity, accuracy, speed and powerful algorithms backed by technical support from Millennium Science and SoftGenetics all but eliminates the need for additional bioinformatics resources. NextGENe Software Easy Gene Annotation and Simple navigation Rapid review of variants, nucleotides and amino acids Flexible mutation scoring system makes of large projects faster and easier Up to ten projects utilized with the same reference can be compared at one time Quick links to NCBI & dbsnp Easy exporting of results Over 15 applications including the Condensation Tool, SNP & INDEL Detection, ChiPSeq Analysis, mirna Discovery, Quantification, Transcriptome Analysis and much more Compatible with all new NGS platforms including the Applied BioSystems SOLiD System, Roche Genome Sequencer FLX and Illumina Genome Analyzer NextGENe software license 8

9 Biological Interpretation Take your NGS results to the next level with Ingenuity Pathway Analysis (IPA) software. IPA helps researchers model, analyze, and understand the complex biological and chemical systems at the core of life science research. IPA has been broadly adopted by the life science research community and cited in hundreds of peer-reviewed journal articles. Ingenuity Pathway Analysis (IPA) Find information about genes, drugs, biomarkers, chemicals, cellular and disease processes, and signaling and metabolic pathways Build dynamic pathway models to quickly and easily model and analyze experimental Unlock the insights buried in experimental by identifying relationships, mechanisms, functions, and pathways of relevance Share customized pathways and results with internal and external collaborators through interactive s, lists, summaries, and pathways Publish research with the support of the most frequently cited pathway solution in the scientific peer-reviewed literature Ingenuity Pathway Analysis software license 9

10 Ordering Information NuGEN prelude tm NUG NUG-1400-A01 prelude direct lysis module (24 reaction kit) prelude direct lysis module (96 reaction kit) NuGEN ovation tm NUG NUG ovation rna-seq system (8 reaction kit) ovation 3 -DGE system (8 reaction kit) fluidigm access array system FLD-AA-PKG-AA Access Array AX + SATC System fld-aa-m access array fld access array loading reagent kit (10 chips) caliper labchip xt system cls labchip xt system cls labchip xt dna 500 kit nugen encore tm nug nug encore ngs system i (8 reaction kit) encore ngs multiplex system i (32 reaction kit) softgenetics nextgene and ingenuity ipa license and price information on enquiry For the latest product, package, price and ordering information, please visit our website at or contact Customer Service at sales@mscience.com.au or call us on in Australia and in New Zealand. Full contact details on back cover. 10

11 Our Genomics Portfolio Redefining the global standard for biosample workflow, transport, and storage solutions. Industry-standard microarray technology for analyzing complex genetic information. Now part of Affymetrix, Panomics offers innovative solutions for Parallel Quantitative Biology. Accelerating the Quest for Significance Developing software and predictive analytical tools for advanced genetic High quality PCR reagents, molecular biology enzymes, and purification kits. Solutions for exploration, interpretation, and of life science information. Developing and distributing systems based on integrated fluidic circuits (IFCs). Fast, high-sensitivity, highthroughput amplification and labeling systems for genomic. Gene expression management and bioinformatic software and services. Effective, biologist friendly, easy-to-use genetic software tools. 11

12 Millennium Science, Pty Ltd Unit 2, 390 Canterbury Road Surrey Hills, Victoria 3127 AUSTRALIA ABN Telephone: Facsimile: Australian Toll Free: New Zealand Toll Free: sales@mscience.com.au Web: