BIOO LIFE SCIENCE PRODUCTS

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1 BIOO LIFE SCIENCE PRODUCTS FOR REFERENCE PURPOSES This manual is for Reference Purposes Only. DO NOT use this protocol to run your assays. Periodically, optimizations and revisions are made to the kit and protocol, so it is important to always use the protocol included with the kit. NEXTflex Small RNA Barcode Primers Set A (Illumina Compatible) Catalog #: (96 reactions) BIOO Scientific Corp V14.07

2 TABLE OF CONTENTS GENERAL INFORMATION... 1 Product Overview... 1 Contents, Storage and Shelf Life... 1 Warnings and Precautions... 1 APPENDIX A... 2 Oligonucleotide Sequences... 2 RELATED PRODUCTS... 3 Illumina Compatible DNA NGS Kits and Adapters... 3 DNA Fragmentation... 4 Illumina Compatible RNA NGS Kits and Adapters... 5 The NEXTflex Small RNA Barcode Primers are intended for laboratory use only. NEXTflex is a trademark of Bioo Scientific.

3 GENERAL INFORMATION GENERAL INFORMATION Product Overview The NEXTflex Small RNA Barcode Primers are designed to prepare multiplexed small RNA libraries for sequencing using Illumina platforms. The index is designed within the NEXTflex barcode primers and incorporated during PCR. Pooling of samples may be performed either after PCR or after gel validation. Contents, Storage and Shelf Life The NEXTflex Small RNA Barcode Primers contain 12 barcoded primers with enough material for 8 reactions each when using the NEXTflex Small RNA Sequencing Kit v2 (catalog numbers and ) or 4 reactions each when using the NEXTflex Small RNA Sequencing Kit* (catalog numbers and ). The shelf life of all reagents is 12 months when stored at -20 C. *If using the NEXTflex Small RNA Sequencing Kit (catalog numbers and ) please make sure to use manual version or later. Please contact Bioo Scientific at the phone number or address shown at the end of this document for the latest manual. Kit Contents Volume NEXTflex Barcode Primer µl Warnings and Precautions Bioo Scientific strongly recommends that you read the following warnings and precautions. Periodically, optimizations and revisions are made to the components and manual. Therefore, it is important to follow the protocol included with the kit. If you need further assistance, you may contact your local distributor or Bioo Scientific at [email protected]. Do not use the kit past the expiration date. Try to maintain a laboratory temperature of C (68 77 F). Vortex and micro centrifuge each tube prior to use, to ensure material has not lodged in the cap or the side of the tube. The NEXTflex Small RNA Barcode Primers are intended for laboratory use only. NEXTflex is a trademark of Bioo Scientific. Bioo Scientific makes no warranty of any kind, either expressed or implied, except that the materials from which its products are made are of standard quality. There is no warranty of merchantability of this product, or of the fitness of the product for any purpose. Bioo Scientific shall not be liable for any damages, including special or consequential damage, or expense arising directly or indirectly from the use of this product. BIOO LIFE SCIENCE PRODUCTS 1

4 APPENDIX A APPENDIX A Possible Causes Recommended Action Oligonucleotide Sequences Poor RNA quality NEXTflex Small RNA Barcode Primers Set A Check the RNA quality before beginning the protocol. NAME SEQUENCE (5 3 ) NEXTflex Barcode Primer CAAGCAGAAGACGGCATACGAGATXXXXXX 1 GTGACTGGAGTTCCTTGGCACCCGAGAATTCCA 1 XXXXXX denotes the index region of adapter. The index sequences and their respective reverse complements of each primer are listed below. NEXTflex Barcode Primer Barcode Sequence Reverse Complement* Barcode Primer 1 CGTGAT ATCACG Barcode Primer 2 ACATCG CGATGT Barcode Primer 3 GCCTAA TTAGGC Barcode Primer 4 TGGTCA TGACCA Barcode Primer 5 CACTGT ACAGTG Barcode Primer 6 ATTGGC GCCAAT Barcode Primer 7 GATCTG CAGATC Barcode Primer 8 TCAAGT ACTTGA Barcode Primer 9 CTGATC GATCAG Barcode Primer 10 AAGCTA TAGCTT Barcode Primer 11 GTAGCC GGCTAC Barcode Primer 12 TACAAG CTTGTA *The reverse complement is the sequence that is reported in the index read. BIOO LIFE SCIENCE PRODUCTS 2

5 RELATED PRODUCTS RELATED PRODUCTS Illumina Compatible DNA NGS Kits and Adapters Product Catalog Number NEXTflex 16S V4 Amplicon-Seq kit NEXTflex 16S V4 Amplicon-Seq kit NEXTflex 16S V4 Amplicon-Seq kit NEXTflex 16S V4 Amplicon-Seq kit NEXTflex 16S V4 Amplicon-Seq kit NEXTflex 16S V4 Amplicon-Seq kit NEXTflex 16S V1-V3 Amplicon-Seq kit NEXTflex 16S V1-V3 Amplicon-Seq kit NEXTflex 16S V1-V3 Amplicon-Seq kit NEXTflex 16S V1-V3 Amplicon-Seq kit NEXTflex 16S V1-V3 Amplicon-Seq kit NEXTflex 16S V1-V3 Amplicon-Seq kit NEXTflex DNA Barcodes NEXTflex DNA Barcodes NEXTflex DNA Barcodes NEXTflex DNA Barcodes NEXTflex-96 DNA Barcodes NEXTflex DNA Sequencing Kit (8 reactions) NEXTflex DNA Sequencing Kit (48 reactions) NEXTflex Rapid DNA Sequencing Kit (8 reactions) NEXTflex Rapid DNA Sequencing Kit (48 reactions) NEXTflex Bisulfite-Seq kit (8 reactions) NEXTflex Bisulfite-Seq kit (48 reactions) NEXTflex Bisulfite-Seq Barcodes NEXTflex Bisulfite-Seq Barcodes NEXTflex Msp 1 (8 reactions) NEXTflex Msp 1 (48 reactions) NEXTflex ChIP-Seq Kit (8 reactions) NEXTflex ChIP-Seq Kit (48 reactions) NEXTflex ChIP-Seq Barcodes NEXTflex ChIP-Seq Barcodes NEXTflex ChIP-Seq Barcodes NEXTflex ChIP-Seq Barcodes NEXTflex-96 ChIP-Seq Barcodes NEXTflex PCR-Free DNA Sequencing Kit (8 reactions) NEXTflex PCR-Free DNA Sequencing Kit (48 reactions) NEXTflex PCR-Free Barcodes NEXTflex PCR-Free Barcodes NEXTflex PCR-Free Barcodes NEXTflex PCR-Free Barcodes NEXTflex Methyl-Seq 1 kit (8 reactions) BIOO LIFE SCIENCE PRODUCTS 3

6 NEXTflex Methyl-Seq 1 kit (48 reactions) DNA Fragmentation Product Catalog Number AIR DNA Fragmentation Kit (10 reactions) AIR DNA Fragmentation Kit (40 reactions) BIOO LIFE SCIENCE PRODUCTS 4

7 Illumina Compatible RNA NGS Kits and Adapters Product Catalog Number NEXTflex Poly(A) Beads (8 reactions) NEXTflex Poly(A) Beads (48 reactions) NEXTflex Poly(A) Beads (100 reactions) NEXTflex RNA-Seq Barcodes NEXTflex RNA-Seq Barcodes NEXTflex RNA-Seq Barcodes NEXTflex RNA-Seq Barcodes NEXTflex-96 RNA-Seq Barcodes NEXTflex RNA-Seq Kit (8 reactions) NEXTflex RNA-Seq Kit (48 reactions) NEXTflex Directional RNA-Seq Kit V2 (8 reactions) NEXTflex Directional RNA-Seq Kit V2 (48 reactions) NEXTflex Rapid RNA-Seq Kit (8 reactions) NEXTflex Rapid RNA-Seq Kit (48 reactions) NEXTflex Rapid Directional RNA-Seq Kit (8 reactions) NEXTflex Rapid Directional RNA-Seq Kit (48 reactions) NEXTflex qrna-seq Kit 4 barcodes (8 reactions) NEXTflex qrna-seq Kit 24 barcodes - Set A (48 reactions) NEXTflex qrna-seq Kit 24 barcodes - Set B (48 reactions) NEXTflex qrna-seq Kit 24 barcodes - Set C (48 reactions) NEXTflex qrna-seq Kit 24 barcodes - Set D (48 reactions) NEXTflex Rapid Directional qrna-seq Kit 4 barcodes (8 reactions) D NEXTflex Rapid Directional qrna-seq Kit 24 barcodes - Set A (48 reactions) D NEXTflex Rapid Directional qrna-seq Kit 24 barcodes - Set B (48 reactions) D NEXTflex Rapid Directional qrna-seq Kit 24 barcodes - Set C (48 reactions) D NEXTflex Rapid Directional qrna-seq Kit 24 barcodes - Set D (48 reactions) D NEXTflex Small RNA Sequencing Kit (24 reactions) NEXTflex Small RNA Sequencing Kit (48 reactions) NEXTflex Small RNA Barcodes Set A NEXTflex Small RNA Barcodes Set B NEXTflex Small RNA Barcodes Set C NEXTflex Small RNA Barcodes Set D Bioo Scientific offers library prep kits and barcodes for the Ion Torrent, 5500 SOLiD and SOLiD 4 sequencing platforms. For more information about any of these kits visit our website at Bioo Scientific Corporation 7050 Burleson Road Austin, TX USA Tel: Fax: (512) Made in USA BIOO Research Products Group [email protected] BIOO LIFE SCIENCE PRODUCTS 5