How To Make A Bacterial Coatings

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1 Antibacterial coatings based on sonochemically designed surface metal nanofoams, 2011, CLG NATO Science for Peace and Security (SPS) Programme Workshop on CBRN Defence October 2013 Brussels Emerging Security Challenges Division NATO Brussels, Belgium,

2 Project Description Antibacterial coatings based on sonochemically designed surface metal nanofoams, 2011, CLG General overview: Advances for a new generation of active metal surfaces with feedback properties. Objectives: Technologies for fabrication of surface metal nanofoams for active chemicals storage by green ultrasound irradiation with combination with selfassembly complexation between biocide and polyelectrolyte in open/close states and formation of surface nanoreservoirs capable of long-term storage and controllable release of the entrapped antimicrobial agents which provide a basis for novel CNBR decontamination coatings. Expected outcomes comprehensible to the non-expert: fabrication of surface encapsulation systems capable of long-term storage and controllable release of the entrapped antimicrobial agents which provide a basis for novel CNBR decontamination coatings. 2

3 What did we want to achieve? Nanoengineered Cavitation Interfaces Understanding and controlling of sonochemical reaction at solid surfaces boost fundamental research, control of nucleation Bio-active Surface Rational interface design inorganic / polymeric / biological antifouling systems, new medical therapies for human transplants, stem-cell research 3

4 What is special in sonochemistry? Hot spot theory High Energy Fast Localised Sonoparameters easy way to various structures Red/Ox vis-à-vis reaction in liquid Additive in liquid single-step hybrids 4

5 Focused ultrasonic treatment * Lower left inset shows multibubble sonoluminecence ** Right inset shows ultrasonic unit for sonochemical modification, where 1) piezoceramic transducer; 2) booster; 3) irradiating horn; 4) gas inlet; 5) thermoelement; 6) thermostated sonocell. 5

6 Cavitation assisted porous surfaces Surface capsules could be formed at higher intensity of sonication 6

7 Surface capsules on metal surface 7

8 Design of intelligent surfaces TEM ultramicrocut of sonomodified metal 8

9 Types of capsules 9

10 Surface pore loading Surface drug delivery: stimuli-responsive, controlled, extended, multi-drug, with biosensing 10

11 Encapsulation forces 11

12 Controllable release behavior 12

13 Specific example of the system Fast creation of reactive groups on both inorganic and organic sides IR spectra Release profile Doxorubicin chemisorption via sonication D.V. Andreeva, D.V. Sviridov, A. Masic. H. Möhwald, E.V. Skorb, SMALL 8 (2012)

14 Specific example of the system before biocide release Release profile after biocide release D.V. Andreeva, D.V. Sviridov, A. Masic. H. Möhwald, E.V. Skorb, SMALL 8 (2012) 820 LIVE/DEAD BacLight bacteria visualisation: inactivated red; living green 14

15 Advances of self-regulated system Immobilization of ph-responsive triblock terpolymer micelles L. lactic bacteria produce lactic acid, ph, micelle s corona size and push off the cells Spatial and temporal resolved cell attraction J. Gensel, T. Borke, N. Pazos- Perez, A. Fery, D.V. Andreeva, E. Betthausen, A. H. E. Müller, H. Möhwald, E.V. Skorb, Adv. Mater. 24 (2012)

16 Project Outcome Antibacterial coatings based on sonochemically designed surface metal nanofoams, 2011, CLG Results (direct scientific and educational successes): Novel ultrasound-assisted method of modification of metal surface and formation of metal nanofoams with high stability, high surface area and high adhesive properties; Novel encapsulation systems with controllable release of biocide and other active compounds; 5 publications in high ranking scientific journals in the field of materials science and nanotechnology, e.g. Adv. Mater., Nanoscale, Small, J. Mater. Chem., Photochem. Photobiol. Sci. Impact : sustainable technology for novel CRBN decontamination coatings; potential application in protective coatings, drug delivery, implants protection, tissue engineering, lab-on-chip, sensors and biosensors. 16

17 Way Forward SPS CBRN Antibacterial coatings based on sonochemically designed surface metal nanofoams, 2011, CLG What research in CBRN Defence requires urgent and substantial attention? A novel nanoengineered systems for manipulation with cells in the bioanalytic lab-on-chip systems. What should be the focus of the SPS Programme in CBRN Defence? Use advances of novel nanoengineered materials, systems fundamental understanding and technological adaptation. Ideas for potential SPS activities in the CBRN field (according to your expertise) Hybrid materials for encapsulation systems, stimuliresponsive surfaces, CBRN decontamination coatings. 17