Pharmaceutical Nanotechnology
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3 Pharmaceutical Nanotechnology
4 Pharmaceutical Nanotechnology Development of Gold-Chitosan-coated PLGA Nanoparticles Marius Hittinger Supervisor: Juniorprofessor Dr. Marc Schneider
5 Pharmaceutical Nanotechnology
6 Gold Nanoparticles Advantages: -Easy to prepare -Special optical properties -Surface modifications possible -Stable -Biocompatible Haiss et al. 2007
7 Gold Nanoparticles Advantages: -Easy to prepare -Special optical properties -Surface modifications possible -Stable -Biocompatible Giljohann et al. 2010
8 PLGA-Chitosan Nanoparticles Advantages: -Easy to prepare -Biodegradable -Biocompatible Nafee et al High efficient drug delivery vehicle -Permeability-enhancing properties (chitosan)
9 Multifunctional Nanoparticles Park et al. 2007
10 Multifunctional Nanoparticles Park et al. 2007
11 Multifunctional Nanoparticles Park et al. 2007
12 Turkevich - Method Kumar et al. 1.! Synthesis 2.! Characterization 3.! Preparation of combined particles 4.! Characterization
13 Turkevich - Method (1951) Ojea-Jiménez et al
14 Turkevich - Method (1951) Ojea-Jiménez et al
15 Preparation of PLGA-Chitosan NPs Kumar et al. 2004
16 Turkevich - Method Kumar et al. 1.! Synthesis 2.! Characterization 3.! Preparation of combined particles 4.! Characterization
17 Preparation - Basic ideas: -Calculate the maximum of particles which can take place on the surface and dilute gold solution before interaction (80%,15%,0%) -Use undiluted gold solution and separate new particles from the AuNP -solution (100%)
18 Preparation - Basic ideas: -Calculate the maximum of particles which can take place on the surface and dilute gold solution before interaction (80%,15%,0%) -Use undiluted gold solution and separate new particles from the AuNP -solution (100%) Filtration Magnetic particles Centrifugation
19 Turkevich - Method Kumar et al. 1.! Synthesis 2.! Characterization 3.! Preparation of combined particles 4.! Characterization
20 Characterization ?
21 SEM TEM AFM NanoSight Characterization IR ? ZetaSizer UV/VIS CLSM
22 What can I expect? Preston et al. 2009
23 What can I expect? Preston et al. 2009
24 What can I expect? Kumar et al Darwich et al. 2011
25 What could be possible? Multifunctional PLGA-Chitosan-Gold-Nanoparticles
26 What could be possible? Multifunctional PLGA-Chitosan-Gold-Nanoparticles
27 What could be possible? -Alginate-Chitosan-Gold-Nanoparticles -Cellular uptake -Microparticles
28 David A. Giljohann, Dwight S. Seferos, Weston L. Daniel, Matthew D. Massich, Pinal C. Patel, and Chad A. Mirkin: Gold Nanoparticles for Biology and Medicine Angew. Chem.(49) Spectra Wolfgang Haiss, Nguyen T. K. Thanh, Jenny Aveyard, and David G. Fernig: Determination of Size and Concentration of Gold Nanoparticles from UV-Vis Anal. Chem.(79) John Turkevich, Peter Cooper Stevenson and James Hillier: A study of the nucleation and growth processes in the synthesis of colloidal gold Discuss. Faraday Soc., 1951, 11, Isaac Ojea-Jimnez, Francisco M. Romero, Neus G. Basts and Victor Puntes: Small Gold Nanoparticles Synthesized with Sodium Citrate and Heavy Water: Insights into the Reaction Mechanism J. Phys. Chem. C, 2010, 114 (4), pp Samer Darwich1, Karine Mougin*1, Akshata Rao2, Enrico Gnecco2, Shrisudersan Jayaraman3 and Hamidou Haidara: Manipulation of gold colloidal nanoparticles with atomic force microscopy in dynamic mode: influence of particle substrate chemistry and morphology, and of operating conditions Beilstein J. Nanotechnol. 2011, 2, Noha Nafee,Sebastian Taetz, Marc Schneider, Ulrich F. Schaefer, Claus-Michael Lehr : Chitosan-coated PLGA nanoparticles for DNA/RNA delivery: effect of the formulation parameters on complexation and transfection of antisense oligonucleotides Nanomedicine: Nanotechnology, Biology, and Medicine (3) LayerThomas C. Preston and Ruth Signorell: Growth and Optical Properties of Gold Nanoshells Prior to the Formation of a Continuous Metallic Layer American Chemical Society VOL. 3 NO Adam D. McFarland, Christy L. Haynes, Chad A. Mirkin, Richard P. Van Duyne, and Hilary A. Godwin: Color My Nanoworld Journal of Chemical Education Vol. 81 No. 4 April 2004 Ravi Kumar*,1, U. Bakowsky, C.M. Lehr: Preparation and characterization of cationic PLGA nanospheres as DNA carriers Biomaterials Huiyul Park, Jaemoon Yang, Sungbaek Seo, Kyujung Kim, Jinsuk Suh, Donghyun Kim, Seungjoo Haam, and Kyung-Hwa Yoo : Multifunctional Nanoparticles for Photothermally Controlled Drug Delivery and Magnetic Resonance Imaging Enhancement small 2008, 4, No. 2,
29 Thanks!
30 Thanks! Pharmaceutical Nanotechnology
31 Free gold nanoparticles easily aggregate when the environment conditions change. Here, gold nanoparticles (AuNPs) with average diameter of 3.7 nm were prepared and then modi#ed with poly(ethylene glycol) (PEG) to improve stability. The gold nanoparticles were #rst surfacemodi#ed with 3-mercaptopropionic acid (MPA) to form a self-assembled monolayer and subsequently conjugated with NH2-PEG-NH2 through amidation between the amine end groups on PEG and the carboxylic acid groups on the particles.
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39 Turkevich - Method Kumar et al.
40 Goldpartikel PLGA-Chitosankern
DNA Assembly and Enzymatic Cutting in Solutions: A Gold Nanoparticle Based SERS Detection Strategy
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