m. Name of Parts 2 I. Standard Set 1 W. Major Troubles with Microscope, their Possible Causes IT. Specifications... 1 VI. Method of Operation...

Transcription

1

2 MODEL KHC INSTRUCTIONS Contents I. Standard Set 1 IT. Specifications m. Name of Parts 2 N. Optical Path V. Structure and Assembly VI. Method of Operation A. Smoothening of Coarse Adjustment knob B. Light Source C. Focusing D. Interpupillary Distance and Diopter Adjustments E. Use of Oil Immersion Objective F. Use of Condenser G. Change of Bulb vll. Optical Characteristics 6 W. Major Troubles with Microscope, their Possible Causes and Applicable Steps to be Taken IX. Important Points to Remember.. 8

3 I. Standard Set Main Body: (built-in light source, stage and revolving nosepiece) Binocular Head Objectives: 4x, lox, 40x, loox, Eyepiece ; Bi WFlOx Condenser Filter Spare Lamps ; 20W Eyepiece tube cap Dustproof Cap (for projection lens) Cargille Oil Vinyl cover Certificate of Inspection 1 set 1 set lea. 1 pr. 1 pc. 1 pc. 3 pcs. 2 pce. 1 pc. 1 btl. 1 pc. 1 copy IT. Specifications Head: Binocular Head Revolving nosepiece : Focusing: Coarse abjustmemt : Fine adjustment: Condenser: Illumination: Stage: Dimensions : 45 0 inclination, rotatable through horizontally; Stopper to fix the head at any designed position. Range of interpupillary distance adjustment: mm. Dioptric adjustment is possible. Quadruple indicator for Objectives' position Vertical movement stage Range of operation 28mm Range of operation 2mm N. A Variable iris-diaphragm Range of vertical movement 15mm Pre-centered 20W light source is built inside the base. Coaxial single-handle square mechanical stage Range of operation: Back-forth 30mm Right-left 70mm Height Width Depth Net weight 355mm 165mm 205mm approx. 5.5kg without case -1-

4 ill. Name of Parts Eyepiece Objective Binocular head Stage Coaxial knob for slide monipulation Condenser Projection lens Coaxial coarse and fine adjustment knobs Filler N. Optical Path Prism Projection Lens Lamp - 2 -

5 V. Structure and Assembly A. Main Body Head mount Head i~ atta('hl~d upon this mount. Arm Slage back-forth movement knob Spl'('iml'n ca" be mo\'t"d back and forth in " rangll of 30mm. Slage right-left movement knob Sp('cimen can be mo\'cd ri..::hl and l.. h in,) ranlo1l' uf 70mm. Pre-set focusing lock Revolving nosepiece Stage clip Stage Condenser mount Condenser locking screw Condenser vertical movement knob Conden~er can hl' mon..j uv and down in a range of lsmm Fine adjustment knob StalCe can be moved \'erticajly in minute mo\'l'mcnt in il range of 2mm. Power source cord Projection lens This is a detachable screw-in type lens. This can be remo\'cd when mirror is used. Illumination switch Push one end of the switch marked with a white dol. to switch on the illuminator. 1. Revolving Nosepiece The quadruple nosepiece moves very lightly and click-stops in the right position for the optical path. Mount the objectives in such an order that, when the nosepiece is turned clockwise during observation, an objective of the next higher magnification will come into position. 2. Pre-set focus lock When the lock is tightened, it will hinder the movement of the stage further upward from that point. When the stage is moved down once and again brought up closer to the lens, it will stop at the position set by the lock. This will help prevent the speciment to crash against the objective and further facilitates an easy focusing for the observer. 3 -

6 3. Condenser Insert the condenser (1) from underneath into the condenser (g) and secure it in position by tightening the locking screw (3J. CD B/';-':Binocular Head Dioptric adjustment ring Turn this ring to adjust the dioptric difference between the right and left eyes. Eyepiece tube seal Holdin~ these seats, pull the lubes apart or push them closer (or interpupilbry distance adjustmpnt. / Eyepiece Interpupillary dislance scale tube Put in the eyepiece. Head mounting ring Clamping screw Tht' head is ixt.'d 10 th.. Th~ head is fixed at tll<' main booy by thi~ ring. desired position by this screw. o How to Mount the Head Place the head on the receptacle at the upper end of the arm and turn the mounting ring te fix it. VI. Method of Operation A. Smoothening of Coarse Abjustment Knob This coarse adjustment knob operates rather heavily However. such can be adjusted to the operator's preference. Hold both knobs firmly, turn them counterclo kwise (as shown in the illustration) to make it operate lightly, and clockwise heavily. B. Light Source Connect the cord (from the base) with the power source. Press the switch on the whitedotted side to turn on the lamp

7 c. Focusing 1. Turn the fine adjustment knob to bring the index line to the middle of the two lines (movement range). 2. Place the specimen on tne stage and secure it with the clips. 3. Put the lox objective in position. 4. Looking from side of the microscope, move the stage upward carefully by the coarse adjustment knob until the specimen is brought very close to the tip of objective. (** Be most careful not to hit the objective.) 5. Looking through the eyepiece, gradually lower the stage to obtain rough focus. 6. Turn the nosepiece to set the desired objective in position. Accrately focus with the fine adjustment knob. 7. The specimen may be moved by the slide manipulation knob, built on the stage so that the particular spot to be observed comes into the center of the view field. **** For convenience in the later operations, tighten up the pre-set focus lock when the specimen is roughly in focus at step 5, above. (See for V -A-2, Pre-set focus lock) D. Inter-pupillary Distance and Dioptor Adjustments 1. The interpupillary distance is adjusted by a. Holding both eyepiece tube seats, b. Looking into the eyepieces, and c. Pulling apart or pushing closer the seats until the view field is observed in one. When the adjustment is made, read the figure on the scale; this will make the adjustment easier from the next time on, as it can be done by merely setting the tubes at the given figure. 2. Diopter is adjusted by a. Looking through the right eyepiece with the right eye, b. Focusing on the specimen by the coarse and fine adjustment knobs, c. Then looking into the left eyepiece with the left eye, and d. Turning the diopter ring until the specimen is brought into focus correctly. When looking into the left eyepiece with the left eye, do not touch the coarse or fine adjustment knob. E. Use of Oil Immersion Objective First, bring the specimen into focus with a low magnification objective (lox). Apply a drop of cargille oil on the specimen as well as at the tip of the oil immersion objective. Turn the nosepiece to bring the oil immersion objective above the specimen. This can be done without the fear of crashing them into each other. Focus accurately with the fine adjustment knob and make observations. Upon completion of observations, carefully wipe off cargille oil on the oil immersion objective with a piece of gauze moistened with xylol. **** Cargille oil left on the objective can ruin the lens greatly. An utmost care is required in this point. -5-

8 F. Use of Condenser If an objective of low magnification (less than 10X) is in use, lower the condenser slightly to avoid an uneven illumination as well as for a better result. The upper-lens of the condenser may be removed. Removal of the upper-lens gives a better result than lowering of the condenser. When the oil-immersion objective is in use, move it up to the top of its movement range. G. Change of Bulb Lay down the microscope, and loosen two screws CD underside the base with a coin or something like that. Remove the cover and change the used lamp with a new one. vi. Optical Characteristics Objective Magnification 4x 10x 40x 1 1 I 100x "", ". Numerical I I aperture (N. A.) 1 1 '" Working distance '"", (W. D.) I I I "'" Eyepiece Focal length "'. I i I "" Total magnification 40x 100x I I WF 10x (Number of viewfield 18) Depth of focus I I I 18.0 I I Actual viewfield 4.5 I I 4.3 I 400x 2.0 I 'OOOX I I 0.45 I

9 * I} ;"'-"".A o.>mt».l v ;...-;;( (.:: C;t f~)m;1j< A? ""C\.\.t To "i'lli/, J; nj.if(1)j'f!f&j v ;..- ;< I.: Ij, 1!*1.1' A->c,':'l:i"o :. tl Ij, ;t Ht v ;..- ;<,.: f,;;'j; ~ n C ".Q fcg 't;.w~~~<ct. ~(1)1!.~~ft~,m $,IJ<fn'J' ~ J:? I.: l&i&" Il;'j (1) fit! ~,it -> t.:. t (1) cei" 0 l&t&",ijlii.)-j"~i': i;i, g.,1i\'(1);jlt~jj ~ L c1> I) :'l: -lta-1.i'. :FJi,\,'''j-ril~(1);1fJ I) ~1fi'~ AtL c 1> I) :'l: i" (1) c e, r.'flll2.(1) 1-., ;(-it,.1jl: Ri ffl L c 'I" ~ "0 OLYMPUS OB-JECTIVE HAS ITS COLOUR BAND THE OLYMPUS OBJECTIVE, you just purchased, has a colour band on it. This new step has been adopted for your convenience, that you may understand the magnification, if you only see the colour, without looking at the magni ficat ion number engraved on the tube. Though there is no ment ion about the colour band in the instruction booklet, upon your recogni t ion of our new colour system, the olympus products wait for your further efficient usage. ~ Colour 1=!/&J JfI /'I'i: ~ If] l-a',t; ~ lffi Biological use Metallurgical use Range of Plan Fl Ach Plan Ach magni ficat ion ~ Purple 1.3X 1.3X 0 -Less than 2X ~ Brown 2X 2X 2X -Less than 4X,n; Red 4X 4X 5X 5X 6X 4 X -Less than 7X.m Orange IOX IOX IOX IOX 7X -Less than 20X 1l. Yellow 20X 20X 20X 20X 20X -Less than 40X '1t~ Brilliant green 40X 40X 40X 40X 40X 40X -Less than 60X,~.f,1c Cobalt blue 60X 60X - Less thanloox ik~j' Light blue loo X loo X loo X loo X loo X loox. and over is* Colour band OLYMPUS OPTICAL CO.. LTD.

10 VIII. Major troubles with microscope, their possible causes and applicable steps to be taken. Troubles I Possible causes 1 Steps to be taken Slipping down of the Loosened coarse See Smoothening of Coarse stage by itself adjustment knob Adjustment knob and properly adjust it. Coarse Adjustment 1. Axis of Coarse Adjust- 1. Take reverse steps of knob too heavy ment knob overly the above. tightened 2. Hardened grease on the 2. Notify the sliding surface. manufacturer. - Fine Adjustment knob Hardened grease on the Notify the manufacturer. too heavy sliding surface Dust/Oirt on objective 1. Eyepiece tubes are left Wipe off the top lens of the without eyepieces or cap. objective with clean gauze; 2. do not touch inner lenses Fingerprints left on the but notify the manufacturer lens; Lens hitting the specimen; or cargille oil left on the oil-immersion lens. Dust/Dirt on eyepiece 1. Careless storage. 1. Be sure to store the eyepieces after use in the provided container or in a desiccator 2. Fingerprints left on the 2. Wipe off with clean lens surfece gauze. Do not try to disassemble the eyepiece. Any change in arrangement of lenses can deteriorate the entire opical efficiency. Scratches on the lens Lens was wiped with a piece Immediately notify the of cloth or leather while manufacturer. The lens will dust or filth is still left on. be replaced at the actual cost

11 IX. Important points to remember Dampness and dust is a taboo for the microscope. But, frequently, a lab where the microscope is used is not free of such. It is the best to store microscope after each use in the provided container. If frequent observations make it impossible, at least cover the instrument with the provided vinyl cover. The objectives and eyepieces should best be stored in a desiccator. Also recommended is to place a pack of silicagel in the container. After the eyepiece is removed from the instrument, be sure to cover the eyepiece tube with the provided cap. Never attempt to disassemble or repair the mechanical parts of the microscope. It must be done by specialists. Cleaning must be performed with utmost care. For expample, dust off with a soft brush or blow off by a rubber ball where hands cannot reach. Mirror (optional accessory) In general, microscopy with KHC is conducted with the projection lens on. However, for special illumination effect this mirror can be used for high magnification lens. and the other side, concave surface, is good for observation with low magnification objl'ctives

12