CyAn : 11 Parameter Desktop Flow Cytometer
|
|
|
- Meghan Pearl Gardner
- 9 years ago
- Views:
Transcription
1 CyAn : 11 Parameter Desktop Flow Cytometer
2 Cyan ADP 3 excitation lines 488nm, 635nm, and UV or violet 11 simultaneous parameters FSC, SSC, and 7-9 colors with simultaneous width, peak, area, and log on each High Throughput >50,000 events/sec analysis rate Desktop footprint
3 Cyan ADP Optical layout
4 Spatially separated laser Lines reduced crosstalk Cyan ADP
5 Cyan ADP Which fluorochromes can we detect? 488nm 2-3 lasers: 488nm, 635nm and 405nm 635nm 405nm
6 Cyan ADP Easy to Change Filters & Dichroics
7 Cyan ADP - Control Cyan ADP controlled by the Summit Software 1. Fluidics 2. Lasers 3. PMTs 4. Trigger and Threshold
8 Cyan ADP - Control 1.Fluidics Sheath management system (SMS) 20-24hours sample run time Event Rate- Fully modifiable Backflush Debubble Both software control panel and LEDs on the SMS provide fluid level status information to the user Automated fluidics startup/shutdown and clean/rinse
9 Cyan ADP - Control Summit Software 2. Lasers Instrument control panel Turn Lasers On and Off Control Laser Shutters
10 Cyan ADP - Control 3, 4. PMTs, trigger and threshold CyAn Parameter Panel Edit the Parameter Names Updated in real-time Change the Voltage on the PMT s Change the Lin, Log, Peak or Integral Setting Change the Gain on Linear Settings Change the Threshold
11 Cyan ADP - Software Summit Software Windows platform Powerful data analysis both during and after acquisition Full Control of All Instrument Parameters Ability to Save an Application Protocol Layout Compensation Settings Parameter Names Voltages, Gains, Lin/Log Free Summit Offline
12 Cyan ADP - Software Boolean gates Overlays Subtraction Normalisation Multiple Sample Analysis Zoom Gate limits and Filters
13 Cyan ADP - Software Drag and Drop 9 x 9 Compensation Matrix Compensation pre- and postacquisition Help function Smoothing/Contours Colours/Fonts Exportable Read/Write all FCS formats
14 Cyan ADP - Applications Multicolour immunophenotyping Rare event detection High speed immunophenotyping Small Particle analysis Signal Transduction DNA/cell cycle analysis
15 Cyan ADP Multicolour immunophenotyping 7-Color Staining Panel Monoclonal Antibodies: CD45 FITC Pan Leukocytes CD56 PE NK marker CD3 PE-TR T cells CD14 PE-Cy5 Monocytes CD4 PE-Cy7 T helper CD19 APC B cells CD8 APC-Cy7 T Suppressor/Cytotoxic Preparation: The cell preparation was carried out on human blood, using the Dako Uti-Lyse kit.
16 Cyan ADP - Mutlicolour immunophenotyping 256 SSC vs. FSC SSC vs. CD3 PE-TR 256 Eosinophils SSC 128 Granulocytes Monocytes Lymphocytes SSC 128 T - Lymphocytes R FSC Debris Leukocyte Populations CD 3 PE-TR Comp
17 Cyan ADP Multicolour immunophenotyping CD14 PE-Cy5 vs. CD45 FITC 10 4 SSC vs. CD45 FITC 256 Monocytes R CD14 PE-Cy5 Comp Neutrophils 10 2 R9 R3 R11 Eosinophils Lymphocytes SSC 128 Lymphocytes R4 R CD 45 FITC Comp CD 45 FITC Comp Basophils Leukocyte Populations
18 Cyan ADP Multicolour immunophenotyping CD19 Positive Lymphocytes APC positive (FL8) Excitation: 635 nm B Cells Detection: 665/20 BP In this donor, 13.0% of the lymphocytes are B cells. B and T cell populations CD 19 APC Comp CD19 APC vs. CD3 PE-TR R12 R13 Gated on Lymphocytes CD 19 APC Comp Natural Killer cells CD19 APC vs. CD56 PE 10 4 R11 R Gated on Lymphocytes NK cells R14 R15 R13 R CD 3 PE-TR Comp T Cells CD 56 PE Comp CD3 Positive Lymphocytes PE-TR positive (FL3) Excitation: 488 nm Detection: 619/20 BP In this donor, 74.1% of the lymphocytes are T cells. CD56 Positive Lymphocytes PE positive (FL2) Excitation: 488 nm Detection: 575/25 LP In this donor, 0.36% of the lymphocytes are NK.
19 Cyan ADP Rare event detection Rare event detection High Speed detection of Antigen (EBV) specific tetramers from whole blood (less than 0.1% total blood cells), with very low background CONTROL EBV+
20 Cyan ADP High speed analysis High Speed Immunophenotyping 1 x 10 6 cells/ml 500,000 events 8 min 18 sec 5 x 10 6 cells/ml 500,000 events 1 min 38 sec 50 x 10 6 cells/ml 500,000 events 10 seconds
21 Cyan ADP Small particle analysis Small particle analysis Bacteria spores, 0.8 µm Fungus, 5 µm Advantage of distinguishing different species of bacteria by light scatter One customer is even looking at levels of bacteria & yeast in wine [2469] 125.0% (SSC) Bacteria spores, 0.5 µm Bacteria, 1-2 µm [2165] 106.3% (FSC)
22 Cyan ADP Signal transduction CFP/YFP FRET Transfected cells CFP only Transfected cells YFP only Transfected cells FRET FSC v SSC gate
23 Cyan ADP - DNA cell viability and cycle analysis DNA Dyes 488 Excited Propidium Iodide (PI) 7-AAD UV Excited DAPI Hoechst 635 Excited DRAQ5 CyAn provides great flexibility as to which laser is used for your DNA measurement
Introduction to flow cytometry
Introduction to flow cytometry Flow cytometry is a popular laser-based technology. Discover more with our introduction to flow cytometry. Flow cytometry is now a widely used method for analyzing the expression
These particles have something in common
These particles have something in common Blood cells Chromosomes Algae Protozoa Certain parameters of these particles can be measured with a flow cytometer Which parameters can be measured? the relative
Katharina Lückerath (AG Dr. Martin Zörnig) adapted from Dr. Jörg Hildmann BD Biosciences,Customer Service
Introduction into Flow Cytometry Katharina Lückerath (AG Dr. Martin Zörnig) adapted from Dr. Jörg Hildmann BD Biosciences,Customer Service How does a FACS look like? FACSCalibur FACScan What is Flow Cytometry?
Multicolor Flow Cytometry: Setup and Optimization on the BD Accuri C6 Flow Cytometer
Multicolor Flow Cytometry: Setup and Optimization on the BD Accuri C6 Flow Cytometer Presented by Clare Rogers, MS Senior Marketing Applications Specialist BD Biosciences 23-13660-00 Webinar Overview Multicolor
Introduction to Flow Cytometry
Introduction to Flow Cytometry presented by: Flow Cytometry y Core Facility Biomedical Instrumentation Center Uniformed Services University Topics Covered in this Lecture What is flow cytometry? Flow cytometer
Cell Cycle Tutorial. Contents
Cell Cycle Tutorial Contents Experimental Requirements...2 DNA Dyes...2 Protocols...3 PI Parameter & Analysis Setup...4 PI Voltage Adjustments...6 7-AAD Parameter Setup...6 To-Pro3 Parameter Setup...6
Introduction to Flow Cytometry
Outline Introduction to Flow Cytometry Basic Concept of Flow Cytometry Introduction to Instrument Subsystems Daisy Kuo Assistant Product Manager E-mail: [email protected] BDBiosciences Application Examples
FlowSight. Flow cytometry with vision
FlowSight Flow cytometry with vision Flow cytometry with vision Introducing FlowSight Capable: Sensitive and flexible for every need Intuitive: Easy-to-use, with imagery for every cell Affordable: Designed
1. Instrument Layout pg 2. 2. Summit Overview pg 3. 3. Data Storage and Data Files pgs 6-7. 4. Saving Data pg 7. 5. Start Up pg 8
Table of Contents 1. Instrument Layout pg 2 2. Summit Overview pg 3 3. Data Storage and Data Files pgs 6-7 4. Saving Data pg 7 5. Start Up pg 8 6. Choosing and Naming Parameters pg 9 7. Creating Histograms
Flow Cytometry. What is Flow Cytometry? What Can a Flow Cytometer Tell Us About a Cell? Particle Size. Flow = Fluid Cyto = Cell Metry = Measurement
What is Flow Cytometry? Flow Cytometry Basic Principle and Applications BD Biosciences Daisy Kuo ([email protected]) Flow = Fluid Cyto = Cell Metry = Measurement A variety of measurements are made on cells,
BD FACSComp Software Tutorial
BD FACSComp Software Tutorial This tutorial guides you through a BD FACSComp software lyse/no-wash assay setup run. If you are already familiar with previous versions of BD FACSComp software on Mac OS
COMPENSATION MIT Flow Cytometry Core Facility
COMPENSATION MIT Flow Cytometry Core Facility Why do we need compensation? 1) Because the long emission spectrum tail of dyes causes overlap like with the fluorophores FITC and PE. 2) For sensitivity reasons,
BD FACSDiva 4.1 - TUTORIAL TSRI FLOW CYTOMETRY CORE FACILITY
BD FACSDiva 4.1 - TUTORIAL TSRI FLOW CYTOMETRY CORE FACILITY IMPORTANT NOTES BEFORE READING THIS TUTORIAL This is a very expensive piece of equipment so PLEASE treat it with respect! After you are done
INSIDE THE BLACK BOX
FLOW CYTOMETRY ESSENTIALS INSIDE THE BLACK BOX Alice L. Givan Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center Dartmouth Medical School HOW NOT TO BE A FLOW CYTOMETRIST Drawing by Ben
No-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Flow Cytometer
APPLICATION NOTE Attune NxT Flow Cytometer No-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Flow Cytometer Introduction Standard methods for isolating and detecting leukocytes
Introduction to Flow Cytometry -- BD LSR II. What is Flow Cytometry? What Can a Flow Cytometer Tell Us About a Cell? Particle Size
Introduction to Flow Cytometry -- BD LSR II Daisy Kuo 郭 正 佼 Assistant Product Manager BD Biosciences [email protected] What is Flow Cytometry? Flow = Fluid Cyto = Cell Metry = Measurement A variety of measurements
Boundary-breaking acoustic focusing cytometry
Boundary-breaking acoustic focusing cytometry Introducing the Attune NxT Acoustic Focusing Cytometer a high-performance system that s flexible enough for any lab One of the main projects in my laboratory
Using the BD TM Cytometer Setup and Tracking (CS&T) System for Instrument Characterization and Performance Tracking
Using the BD TM Cytometer Setup and Tracking (CS&T) System for Instrument Characterization and Performance Tracking Mark KuKuruga Senior Technical Applications Specialist BD Biosciences 23-14462-00 Outline
APPLICATION INFORMATION
DRAFT: Rev. D A-2045A APPLICATION INFORMATION Flow Cytometry 3-COLOR COMPENSATION Raquel Cabana,* Mark Cheetham, Jay Enten, Yong Song, Michael Thomas,* and Brendan S. Yee Beckman Coulter, Inc., Miami FL
FACS Laboratory. BD LSRs Operator Training. Training Guide. http://london-research-institute.org.uk/technologies/facs
FACS Laboratory [email protected] Training Guide http://london-research-institute.org.uk/technologies/facs BD LSRs Operator Training LRI FACS lab 1 Components 1.1 Basic parts overview.pg3-6 Overview
Instructions for Use. CyAn ADP. High-speed Analyzer. Summit 4.3. 0000050G June 2008. Beckman Coulter, Inc. 4300 N. Harbor Blvd. Fullerton, CA 92835
Instructions for Use CyAn ADP High-speed Analyzer Summit 4.3 0000050G June 2008 Beckman Coulter, Inc. 4300 N. Harbor Blvd. Fullerton, CA 92835 Overview Summit software is a Windows based application that
CyFlow Space Your flexible flow cytometer
Your flexible flow cytometer www.sysmex-partec.com its flexibility gives you the space you need for your work Analysing cells and particles, be it from blood, plasma, tissue, plants, cell cultures or other
CyFlow SL. Microbiology. Detection and Analysis of Microorganisms and Small Particles
CyFlow SL Microbiology Detection and Analysis of Microorganisms and Small Particles 01 COMPANY Flow Cytometry made in Germany New sophisticated applications and increasing requirements for reliable results
Instrument Characterization and Performance Tracking for Digital Flow Cytometers
Instrument Characterization and Performance Tracking for Digital Flow Cytometers BD Biosciences Cytometer Set-up & Tracking (CS&T) System For Research Use Only. Not for use in diagnostic or therapeutic
Analyzer Experiment setup guide for LSRII and Canto s
Analyzer Experiment setup guide for LSRII and Canto s 1. Check the instrument configuration on our website to determine the most appropriate cytometer for your experiment, https://depts.washington.edu/flowlab/instrumentation.html,
MEASURABLE PARAMETERS: Flow cytometers are capable of measuring a variety of cellular characteristics such as:
INTRODUCTION Flow Cytometry involves the use of a beam of laser light projected through a liquid stream that contains cells, or other particles, which when struck by the focused light give out signals
Three Rules for Compensation Controls
From FlowJo s Daily Dongle Three Rules for Compensation Controls First and foremost, there must be a single-stained control for every parameter in the experiment! In addition, there are three rules for
Flow Cytometry for Everyone Else Susan McQuiston, J.D., MLS(ASCP), C.Cy.
Flow Cytometry for Everyone Else Susan McQuiston, J.D., MLS(ASCP), C.Cy. At the end of this session, the participant will be able to: 1. Describe the components of a flow cytometer 2. Describe the gating
Immunophenotyping peripheral blood cells
IMMUNOPHENOTYPING Attune Accoustic Focusing Cytometer Immunophenotyping peripheral blood cells A no-lyse, no-wash, no cell loss method for immunophenotyping nucleated peripheral blood cells using the Attune
powerful. Intuitive. Customizable. The new star of benchtop flow cytometry
powerful. Intuitive. Customizable. The new star of benchtop flow cytometry About ACEA Established in 2002, ACEA Biosciences, Inc. develops cutting-edge cell analysis platforms for life science research.
Science is hard. Flow cytometry should be easy.
Science is hard. Flow cytometry should be easy. TABLE OF CONTENTS 1 INTRODUCTION TO BD ACCURI C6 SOFTWARE... 1 1.1 Starting BD Accuri C6 Software... 1 1.2 BD Accuri C6 Software Workspace... 2 1.3 Opening
CELL CYCLE BASICS. G0/1 = 1X S Phase G2/M = 2X DYE FLUORESCENCE
CELL CYCLE BASICS Analysis of a population of cells replication state can be achieved by fluorescence labeling of the nuclei of cells in suspension and then analyzing the fluorescence properties of each
EDF Extended Depth of Field
EDF Extended Depth of Field An upgrade for the ImageStream system Think outside the dot. Break the classic depth of field barrier Improve precision Enhance discrimination Simplify analysis Increase resolution
Spherotech, Inc. 27845 Irma Lee Circle, Unit 101, Lake Forest, Illinois 60045 1
78 Irma Lee Circle, Unit 0, Lake Forest, Illinois 00 SPHERO TM Technical Note STN- Rev B 008 DETERMINING PMT LINEARITY IN FLOW CYTOMETERS USING THE SPHERO TM PMT QUALITY CONTROL EXCEL TEMPLATE Introduction
Application Information Bulletin: Set-Up of the CytoFLEX Set-Up of the CytoFLEX* for Extracellular Vesicle Measurement
Application Information Bulletin: Set-Up of the CytoFLEX Set-Up of the CytoFLEX* for Extracellular Vesicle Measurement Andreas Spittler, MD, Associate Professor for Pathophysiology, Medical University
CyFlow SL. Healthcare Immunology. Portable FCM System for 3-colour Immunophenotyping
CyFlow SL Healthcare Immunology Portable FCM System for 3-colour Immunophenotyping 01 COMPANY Flow Cytometry made in Germany New sophisticated applications and increasing requirements for reliable results
Hoechst 33342 HSC Staining and Stem Cell Purification Protocol (see Goodell, M., et al. (1996) J Exp Med 183, 1797-806)
Hoechst 33342 HSC Staining and Stem Cell Purification Protocol (see Goodell, M., et al. (1996) J Exp Med 183, 1797-86) The Hoechst purification was established for murine hematopoietic stem cells (HSC)
Principles of Flowcytometry
Objectives Introduction to Cell Markers: Principles of Flowcytometry Michelle Petrasich NZIMLS Scientific Meeting August 24, 2010, Paihia What are cell markers How do we detect them Production of Monoclonal
Flow cytometry basics fluidics, optics, electronics...
Title Flow cytometry basics fluidics, optics, electronics... RNDr. Jan Svoboda, Ph.D. Cytometry and Microscopy Core Facility IMB, CAS, v.v.i Vídeňská 1083 Fluorescence Fluorescence occurs when a valence
Standard Operating Procedure
1.0 Purpose: 1.1 The characterisation of of main leukocyte subsets in peripheral blood cells from mice by flow cytometry. Reliable values of frequencies of leukocyte clusters are very much dependent on
LSR II Users Guide Stanford Shared FACS Facility 06/10/13
LSR II Users Guide Stanford Shared FACS Facility 06/10/13 A configuration sheet is located on the Website in the Instrument Overview area Do I need to run beads when I collect data using Diva Digital?
123count ebeads Catalog Number: 01-1234 Also known as: Absolute cell count beads GPR: General Purpose Reagents. For Laboratory Use.
Page 1 of 1 Catalog Number: 01-1234 Also known as: Absolute cell count beads GPR: General Purpose Reagents. For Laboratory Use. Normal human peripheral blood was stained with Anti- Human CD45 PE (cat.
Using BD FACSDiva CST To. Evaluate Cytometer Performance, Create Custom Assay Settings. and
Using BD FACSDiva CST To Evaluate Cytometer Performance, Create Custom Assay Settings and Implement Cross-Instrument and Cross-Site Standardization of Assays PART 2 Alan M. Stall Director, Advanced Cytometry
Introduction to Flow Cytometry:
Introduction to Flow Cytometry: A Learning Guide Manual Part Number: 11-11032-01 April, 2000 BD Biosciences 2350 Qume Drive San Jose, CA 95131-1807 1-800-448-2347 Introduction to Flow Cytometry: A Learning
FLOW CYTOMETRY: PRINCIPLES AND APPLICATIONS. By: Douaa Moh. Sayed
FLOW CYTOMETRY: PRINCIPLES AND APPLICATIONS By: Douaa Moh. Sayed Definition Flow cytometry is a technique for counting, examining, and sorting microscopic particles suspended in a stream of fluid. It allows
Application Note. Selecting Reagents for Multicolor Flow Cytometry. Holden Maecker and Joe Trotter BD Biosciences, San Jose
Selecting Reagents for Multicolor Flow Cytometry Holden Maecker and Joe Trotter BD Biosciences, San Jose Contents 1 The basics: Know your instrument 2 Fluorochromes: Go for the bright 3 Colors and specificities:
Below is a list of things you should be aware of before you schedule your sort.
Sorting at the Flow Cytometry Facility At the present time, the assistance of a trained cell sorter operator is needed for all sorting applications. As such, if you are planning a first time sort you will
Technical Bulletin. Threshold and Analysis of Small Particles on the BD Accuri C6 Flow Cytometer
Threshold and Analysis of Small Particles on the BD Accuri C6 Flow Cytometer Contents 2 Thresholds 2 Setting the Threshold When analyzing small particles, defined as particles smaller than 3.0 µm, on the
Immunophenotyping Flow Cytometry Tutorial. Contents. Experimental Requirements...1. Data Storage...2. Voltage Adjustments...3. Compensation...
Immunophenotyping Flow Cytometry Tutorial Contents Experimental Requirements...1 Data Storage...2 Voltage Adjustments...3 Compensation...5 Experimental Requirements For immunophenotyping with FITC and
CELL CYCLE BASICS. G0/1 = 1X S Phase G2/M = 2X DYE FLUORESCENCE
CELL CYCLE BASICS Analysis of a population of cells replication state can be achieved by fluorescence labeling of the nuclei of cells in suspension and then analyzing the fluorescence properties of each
UNIVERSITY OF PÉCS MEDICAL SCHOOL FLOW CYTOMETRY AND CELL SEPARATION BIOPHYSICS 2. 2015 4th March Dr. Beáta Bugyi Department of Biophysics Flow cytometry and cell separation FLOW = STREAM OF FLUID in a
Easy to use. Compact. Flexible. Sorting Chip
SH800Z Cell Sorter The SH800Z is a compact, affordable, and easy to use cell sorter developed using the state- of-the art technologies from Sony. An array of automated mechanisms makes it an ideal sorter
Technical Bulletin. Standardizing Application Setup Across Multiple Flow Cytometers Using BD FACSDiva Version 6 Software. Abstract
arch 212 Standardizing Application Setup Across ultiple Flow Cytometers Using BD FACSDiva Version 6 Ellen einelt, ervi Reunanen, ark Edinger, aria Jaimes, Alan Stall, Dennis Sasaki, Joe Trotter Contents
UCHC Guide to CellQuest Pro. FACS Calibur instruments
UCHC Guide to CellQuest Pro FACS Calibur instruments Startup: Turn on Cytometer using green switch on the side of the cytometer Cytometer Power Switch Within a minute of turning on the cytometer; turn
Selected Topics in Electrical Engineering: Flow Cytometry Data Analysis
Selected Topics in Electrical Engineering: Flow Cytometry Data Analysis Bilge Karaçalı, PhD Department of Electrical and Electronics Engineering Izmir Institute of Technology Outline Compensation and gating
HIGH PERFORMANCE FLOW CYTOMETER FOR EVERYONE
POWERFUL. INTUITIVE. CUSTOMIZABLE. HIGH PERFORMANCE FLOW CYTOMETER FOR EVERYONE ABOUT ACEA Established in 2002, ACEA Biosciences, Inc. develops cutting-edge cell analysis platforms for life science research.
MACSQuantify Software guide. Basics of flow cytometric analysis and software guide. Version 1 EN Original instructions
MACSQuantify Software guide Basics of flow cytometric analysis and software guide Version 1 EN Original instructions Miltenyi Biotec GmbH Friedrich-Ebert-Straße 68 51429 Bergisch Gladbach Germany Phone:
Flow Cytometry. flow cytometer DNA apoptosis ph
Flow Cytometry flow cytometer DNA apoptosis ph flow cytometry flow cytometer (a) (b) cell counting instrument (c) 1960 ink-jet technology 17 19 1940 1950 fluorescence microscopy fluorescent dye DNA polyclonal
Uses of Flow Cytometry
Uses of Flow Cytometry 1. Multicolour analysis... 2 2. Cell Cycle and Proliferation... 3 a. Analysis of Cellular DNA Content... 4 b. Cell Proliferation Assays... 5 3. Immunology... 6 4. Apoptosis... 7
Technical Bulletin. An Introduction to Compensation for Multicolor Assays on Digital Flow Cytometers
An Introduction to Compensation for Multicolor Assays on Digital Flow Cytometers BD Biosciences, San Jose, CA Contents 2 Introduction to compensation 5 Tips for setting up compensation 10 Tools for easy,
Stepcount. Product Description: Closed transparent tubes with a metal screen, including a white matrix at the bottom. Cat. Reference: STP-25T
Product Description: Closed transparent tubes with a metal screen, including a white matrix at the bottom Cat. Reference: STP-25T Reagent provided:: 25 Stepcount tubes for 25 test INTENDED USE. Immunostep
The NIAID Flow Cytometry Advisory Committee; the Guidelines Subcommittee
January, 1999 From: To: Subject: The NIAID Flow Cytometry Advisory Committee; the Guidelines Subcommittee NIAID DAIDS Flow Cytometry Laboratories Comparison study information for labs wishing to switch
Supplementary Material. Free-radical production after post-thaw incubation of ram spermatozoa is related to decreased in vivo fertility
10.1071/RD14043_AC CSIRO 2015 Supplementary Material: Reproduction, Fertility and Development, 2015, 27(8), 1187 1196. Supplementary Material Free-radical production after post-thaw incubation of ram spermatozoa
Partec PAS. Your Flow Cytometer.
Partec PAS Your Flow Cytometer. The Challenge... New sophisticated applications and increasing requirements for reliable routine results in the shortest possible time continue to impose a challenge for
CyAn ADP with Summit Software User Guide
CyAn ADP with Summit Software User Guide Document Number 0000050 Revision F July 2006 Copyright 2001-2006 Dako. All rights reserved. This document may not be copied in whole or in part or reproduced in
Flow Cytometry A Basic Overview
Flow Cytometry A Basic Overview Overview Flow cytometry is a powerful technology for investigating many aspects of cell biology and for isolating cells of interest. Flow cytometry utilizes highly focused,
FACSCanto RUO Special Order QUICK REFERENCE GUIDE
FACSCanto RUO Special Order QUICK REFERENCE GUIDE INSTRUMENT: 1. The computer is left on at all times. Note: If not Username: Administrator Password: BDIS 2. Unlock the screen with your PPMS account (UTSW
BD FACSCanto Flow Cytometer. Efficient Easy Powerful
BD FACSCanto Flow Cytometer Efficient Easy Powerful The BD FACSCanto System: Optimal Workflow and Time Management Automated compensation algorithms with optional off-line compensation, streamlined sample
UCHC Guide to FACS DiVa. LSR II instruments
UCHC Guide to FACS DiVa LSR II instruments Start Up: Turn on the instrument using the Green power button. Fluidics pump power, LSR II-B & C LSR II-B and LSRII-C-> Turn on the fluidics pump and ensure that
GETTING STARTED. 6. Click the New Specimen icon.
BD FACSDiva Software Basic Experiment Guide Please note, this guide is not intended to take the place of in-person training but is to be used as a reference as needed after completing training with Unified
Flow Data Analysis. Qianjun Zhang Application Scientist, Tree Star Inc. Oregon, USA FLOWJO CYTOMETRY DATA ANALYSIS SOFTWARE
Flow Data Analysis Qianjun Zhang Application Scientist, Tree Star Inc. Oregon, USA Flow Data Analysis From Cells to Data - data measurement and standard Data display and gating - Data Display options -
A Basic Guide. Institute of Medical Biology, University of Southern Denmark. Ungated Gate 1 (= R1) Gate 4 (=R4) Gate3 (=R3) Gate 2 (= R2) R3 R4
Flow Cytometry A Basic Guide R1 Ungated Gate 1 (= R1) R2 R3 R4 Gate 2 (= R2) Gate3 (=R3) Gate 4 (=R4) Graham Leslie, PhD Institute of Medical Biology, University of Southern Denmark Graham Leslie, PhD
Lab 2. Isolation of mononuclear cells from peripheral blood and separation into subpopulations
Lab 2 Isolation of mononuclear cells from peripheral blood and separation into subpopulations Supervisors: Sissela Broos [email protected] tel: 222 96 78 Niclas Olsson [email protected]
Standardization, Calibration and Quality Control
Standardization, Calibration and Quality Control Ian Storie Flow cytometry has become an essential tool in the research and clinical diagnostic laboratory. The range of available flow-based diagnostic
Multicolor Bead Flow Cytometry Standardization Heba Degheidy MD, PhD, QCYM DB/OSEL/CDRH/FDA Manager of MCM Flow Cytometry Facility
Multicolor Bead Flow Cytometry Standardization Heba Degheidy MD, PhD, QCYM DB/OSEL/CDRH/FDA Manager of MCM Flow Cytometry Facility The mention of commercial products, their sources, or their use in connection
Flow Cytometry 101. Jodi Moore, PhD Jan 2012 Goldenson 113
Flow Cytometry 101 Jodi Moore, PhD Jan 2012 Goldenson 113 Origins:? Dye Chemistry 1940-1980 s Electronics Microscopy Automated Clinical Cytology ID Apps Computers Cytometry vs. Flow Cytometry Microspectrophotometry
Compensation Controls Data Visualization Panel Development Gating Quantum Dots
Compensation Controls Data Visualization Panel Development Gating Quantum Dots FITC Single Stain Control FITC PE Argon Laser FL1 FL2 450 500 550 600 FITC Compensation Control Unwanted signal detected in
How to operate the BD FACSCanto flow cytometer
How to operate the BD FACSCanto flow cytometer Preface Dear colleague, the BD FACSCanto flow cytometer is use to operate, however, it is a delicate instrument for measuring fluorescence of single cells.
Compensation in Flow Cytometry
Compensation in UNIT.4 The term compensation, as it applies to flow cytometric analysis, refers to the process of correcting for fluorescence spillover, i.e., removing the signal of any given fluorochrome
Fluorescence Compensation. Jennifer Wilshire, PhD [email protected]
Fluorescence Compensation Jennifer Wilshire, PhD [email protected] Outline 1. Compensation Basics 2. Practical Applications -selecting comp controls (cells, stains?) 3. Multicolor Issues -spreading due
Islet Viability Assessment by Single Cell Flow Cytometry
Islet Viability Assessment by Single Cell Flow Cytometry Page 1 of 8 Purpose: To comprehensively assess the viability of the islet cell preparation prior to transplantation. Tissue Samples: A sample containing
Small-Particle Analysis
John Sharpe, PhD, and Sonja Wulff Introduction Flow cytometry instrumentation is a powerful tool for the analysis and identification of complex samples of particles of significantly varying size. However,
ab139418 Propidium Iodide Flow Cytometry Kit for Cell Cycle Analysis
ab139418 Propidium Iodide Flow Cytometry Kit for Cell Cycle Analysis Instructions for Use To determine cell cycle status in tissue culture cell lines by measuring DNA content using a flow cytometer. This
BD LSR II and FACSDiVa Software. Dr. Jens Fleischer, Basel Dr. Norbert Leclere,, Berlin
BD LSR II and FACSDiVa Software, Basel Dr. Norbert Leclere,, Berlin Overview Electronics Covers Fluidics Connectors Controlpanel Sample Port On/Off The Control Panel Ease of Use The basic procedures to
EdU Flow Cytometry Kit. User Manual
User Manual Ordering information: (for detailed kit content see Table 2) EdU Flow Cytometry Kits for 50 assays: Product number EdU Used fluorescent dye BCK-FC488-50 10 mg 6-FAM Azide BCK-FC555-50 10 mg
Setting up and calibration of a flow cytometer for multicolor immunophenotyping
Journal of Biological Regulators and Homeostatic Agents Setting up and calibration of a flow cytometer for multicolor immunophenotyping J. KRAAN 1, J.W. GRATAMA 1, M. KEENEY 2, J.L. D HAUTCOURT 3 1 Department
flow cytometry reinvented
FLOW CYTOMETRY flow cytometry reinvented The Attune Acoustic Focusing Cytometer Precision and at all speeds for rare events and precious samples The Attune Acoustic Focusing Cytometer Precise high collection
Cytometry without limits
Cytometry without limits The ImageStream X Mark II Imaging Flow Cytometer Get phenotype and functional insights, even with the rarest cells The new Amnis ImageStream X Mark II imaging flow cytometer combines
BD CellQuest Pro Software Analysis Tutorial
BD CellQuest Pro Analysis Tutorial This tutorial guides you through an analysis example using BD CellQuest Pro software. If you are already familiar with BD CellQuest Pro software on Mac OS 9, refer to
BD FACSVerse. Simply brilliant
BD FACSVerse Simply brilliant BD FACSVerse: Simply Brilliant The BD FACSVerse flow cytometer was engineered from the ground up to offer remarkable performance, flexibility, and ease of operation for research
FRET Basics and Applications an EAMNET teaching module
FRET Basics and Applications an EAMNET teaching module Timo Zimmermann + Stefan Terjung Advanced Light Microscopy Facility European Molecular Biology Laboratory, Heidelberg http://www.embl.de/almf/ http://www.embl.de/eamnet/
International Beryllium Conference, Montreal, Canada March 10, 2005
Alternative Lymphocyte Proliferation Tests: BrdU and Flow Cytometry Based Tests International Beryllium Conference, Montreal, Canada March 10, 2005 Tim K. Takaro Department of Environmental and Occupational
Bacterial Detection and Live/Dead Discrimination by Flow Cytometry
A P P L I C A T I O N N O T E Bacterial Detection and Live/Dead Discrimination by Flow Cytometry M I C R O B I A L C Y T O M E T R Y BD FACSCalibur, BD FACSort Flow Cytometers Introduction Accurate determination
Granulocytes vs. Agranulocytes
Leukocytes are white blood cells (AKA colorless (non-pigmented) blood cells). (Much) smaller in number than RBCs. Unlike RBCs, there are several different types of WBCs. All contain a visible nucleus.
BD FC Beads 8-Color Kit for BD OneFlow Assays
BD FC Beads 8-Color Kit for BD OneFlow Assays 7/2014 23-15785-00 IVD 5 tests per kit Catalog No. 658621 BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. 2014 BD Becton,
Application Note 10. Measurement of Cell Recovery. After Sorting with a Catcher-Tube-Based. Cell Sorter. Introduction
Application Note 10 Measurement of Cell Recovery After Sorting with a Catcher-Tube-Based Cell Sorter Introduction In many experiments using sorted cells, it is important to be able to count the number
Flow Cytometry Protocols
METHODS IN MOLECULAR BIOLOGY TM TM Volume 263 Flow Cytometry Protocols SECOND EDITION Edited by Teresa S. Hawley Robert G. Hawley 1 Flow Cytometry An Introduction Alice L. Givan Summary A flow cytometer
Institut für Experimentelle Immunologie und Bildgebung
Page 1 von 6 Starting Up the System Start up the computer, start Windows and log in as IMCES_user start BD FACSDiva software with your Groupname Turn on the cytometer main power Wait a moment and Check
--Verity Software House, ModFit for Proliferation http://www.vsh.com/ offers online tutorials
Customers may want to use another analysis software package with data collected on Attune. Reason for this include: they already have the SW and are confortable with using it, their institution may provide