Point-of-Care devices

Transcription

1 Point-of-Care devices Winnie E. Svendsen DTU Nanotech

2 Point of Care - Health org/about-the-alliance

3 Point of Care Health

4 Point of Care Technology Ref: Center for Bio photonics Science and technology University of California

5 Point of Care Enviroment 2013 Vandforurening i Kalundborg Børnehave ramt af vandforurening på tredje uge Udgivet den : Stadig forbud mod brug af vand i centrum af Kalundborg Udgivet den :09 af TV2 ØST's Redaktion 2011 Advarsel: Drik ikke vandet i KBH Fredag d. 19. aug kl. 17:11

6 Point of Care Enviroment The Law : Lab test 30 test per year 72 hours response time

7 Nano Bio Integrated Systems (NaBIS ) Micro and Nano Sensor Platforms Microfluidic Sample handling and manipulation Bio Medical Applications 2012 Our vision is to incorporate our research in point of care (POC) technology for diagnostic and monitoring. This is achieved by application-driven research, adapting and refining methodologies to micro- and nanotechnology within bio-medical fields

8 Nano Bio Integrated Systems (NaBIS ) Micro and Nano Sensor Platforms Microfluidic Sample handling and manipulation Bio Medical Applications

9 Point of Care Sensors Micro and Nano Sensor Platforms Microfluidic Sample handling and manipulation Bio Medical Applications

10 Point of Care Sensors

11 Nano Bio sensors - Health A screenning system for The plasmacytosis virus in Mink Biomedical engineering for cancer and brain disease diagnosis and therapy development

12 64 chips The Silicon Nano Wires Sensor SiNW h= 50 nm l = 200 µm w= 50 µm 300 nm 4 SiNW on one chip

13 SiNW h= 50 nm l = 200 µm w= 50 µm Azeem Zulfigar

14 Semiconducting Nanowire Sensor Know and can control charge carriers in the wire Acceptors Holes (mobile) Receptors binding a target molecule (negative) => Get a chage in conductance (DG/G)

15 Silicon Nanowire Biosensor Nanowire Add a functional layer Multiple functional layers

16 Silicon Nanowire Biosensor

17 SiNW sensor for Aleutian Disease Virus (ADV) Integration of Silicon Nanowires in Microfluidic Devices

18 SiNW sensor for Aleutian Disease Virus (ADV) Aleutian Disease or Mink Plasmacytosis is a highly contagious parvovirus affecting the animals, causing spontaneous abortion and death in minks and ferrets. ADV smallest virus existing d~7 nm Task : Screen blood samples for antibodies

19 Immobilization of whole virus APTES Goal : Attach the dead virus on the surface Detect antibodies in the animal sample

20 Test Functionalisation Scheme Immobilization results 12 hours without light 12 hours with light 3 hours with P H 3 CBF 4 Conclusion: 1.High selectivity 2.High Yield 3.The catalysts worked as planned

21 Screening for ADV virus in Minks sample Lars Andresen KU Sund 2010

22 Integration of Silicon Nanowires in Microfluidic Devices Michael Jørgensen Msc Martin H. Sørensen MSc

23 Fully automated system

24 POCT Screening of Cancer Biomedical engineering for cancer and brain disease diagnosis and therapy development CA9 NKG2D ligands

25 Functionalisation Scheme APTES Andrea DTU Lars KU Sund

26 Mouse Antibody Goat Antibody interaction

27 Sample Handling Andrea Pfreundt DTU Nanotech

28 System Engineering/ Embedded Systems Jan Madsen Francois Patou DTU Compute DTU NTCH

29 People NaBIS Acnowledgements Collaborators DTU Nanotecth Mads Brandbyge Kristian Mølhave Funds KU-life DTU Farma DPA isas Slovakia DTU Compute Søren Schou Lars Andresen Franziska, Phd student Åse Uttenthal Leif Bruun Rebekka Dam-Tuxen, Phd Stud. Prof. Silvia Patrokova Prof. Jan Madsen