SOLID PHASE EXTRACTION UCT FORENSICS. Green Earth page

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1 FORENSICS Green Earth page 4 SOLID PHASE EXTRACTION P R O D U C T S C ATA L O G

2 2 is a respected leader in the drug testing, pharmaceutical, clinical, environmental and agricultural industries. Our wide range of highly reproducible solid phase extraction columns allow the chromatographer a consistent extraction technique, and our expertise in silane manufacturing allows a greater control of the chemical processes involved in producing our high quality bonded phase. We manufacture our complete product line of bonded phases. We manufacture our complete product line of bonded silica sorbents, packaged in a variety of formats, including SPE columns, 96 & 48 well plates, universal cartridges and micro centrifuge tubes. We also offer a variety of SPE accessories including derivatizing reagents, GC liners, and manifolds. FORENSICS CLINICAL VETERINARY PHARMACEUTICAL Since our beginnings in 1986, has evolved into a major competitor in the field of silica based solid phase extraction technology. Our commitment to ensuring the satisfaction of our customers is accomplished by delivering on our promises: top-quality, dependable solid phase extraction products, and unmatched technical support. FORENSICS

3 CUSTOMER SERVICE PRICES AND TERMS Our prices are subject to change without notice. The price in effect when we receive your order will apply. All prices are in US Dollars and are F.O.B. Lewistown, PA Terms of payment are net 30 days. MINIMUM ORDERS We welcome all orders, therefore, we do not have a minimum order requirement. When ordering, please include your purchase order number, complete Ship To and Bill To address, catalog number, quantity, and description of product(s). Also include your name and a phone number where you can be reached should we have any questions concerning your order. Custom items will be evaluated on an individual basis; quantity requirements may be necessary. SHIPMENTS Normal processing is within hours after receipt of an order. Unless special shipping requests have been made, our trained staff will send all orders by UPS Ground service. The appropriate shipping charges (freight & insurance costs) will be added to the invoice, unless otherwise instructed by the customer. SPECIAL PRICING We offer special pricing for volume purchases and standing orders. Please call a sales representative for more information on special pricing qualifications. RETURN POLICY Our Quality Manager will handle all returns. Before returning merchandise, please call to obtain a return authorization number from your sales representative. We will need to know the reason for the return, date of purchase, purchase order number and invoice number in order to issue a return authorization number. Returned merchandise must be received before a credit can be issued. Returns will not be accepted after 90 days. A restocking fee of 25% of the price paid, or a minimum of $25.00 (whichever is greater) will be charged on all returns. WARRANTY All products manufactured by are guaranteed against defects in materials and workmanship for a period of 90 days after shipment. will replace any items that prove to be defective during this time period. The exclusive remedy requires the end user to first advise of the defective product by phone or in writing. Secondly, the defective product must be returned within 30 days after proper approval from our Quality Manager. All returns must indicate the purchase order number, the lot number and the shipping date. s total liability is limited to the replacement cost of products. This warranty does not apply to damage resulting from misuse. 3 Contact Us Phone: Fax: , Inc Bartram Rd Bristol, PA [email protected] Web:, Inc Bartram Rd Bristol, PA USA

4 4 w w w. u n i t e d c h e m. c o m A GREENER EARTH Here at, Inc. we are making an effort to keep the planet cleaner and greener for everyone. It is our belief that we must act now to preserve our environment for future generations to come. Organizations we support: Arbor Day Foundation Audubon Society Sierra Club, Inc Bartram Rd Bristol, PA USA

5 TABLE OF CONTENTS Solid Phase Extraction Phases Reservoirs for Bonded Phase Extractions...9 Use of Bonded Phases for Sample Preparation...10 CLEAN SCREEN Sample Prep Products Mechanism of CLEAN SCREEN...12 Copolymeric Bonded Phases for Drug Abuse Testing...13 CLEAN SCREEN XCEL CLEAN SCREEN FASt...16 CLEAN SCREEN ETG / BNZ and CLEAN-THRU TIPS...17 CLEAN SCREEN RSV XtrackT HIGH-FLOW Bonded Phases CLEAN-UP Solid Phase Extraction Phases Hydrophobic Extraction Columns Hydrophilic Extraction Columns Ion Exchange Extraction Columns A Map to Optimizing Sample Clean Up using Solid Phase Extraction Copolymeric Extraction Columns Covalent Extraction Columns...46 STYRE SCREEN Polymeric Resin Extraction Columns Benzenesulfonic Acid + C18, Polystyrene Divinylbenzene, Ion Exchange, Reverse Phase, Carboxylic Acid and Quaternary Amine SELECTRASORB TM Bulk Sorbents Reservoirs Frits...53 Solid Phase Extraction Accessories...54 Positive Pressure Manifold Universal Vacuum Manifold and Accessories Vacuum Manifold and Accessories Select ph Buffer Pouches...60 SELECTRA-SIL Derivatizing Reagents GC Liners...66 SELECTRA HPLC Columns Deep Well Plates Deep Well Plates Flash Chromatography Columns , Inc Bartram Rd Bristol, PA USA

6 SOLID PHASE EXTRACTION PHASES CT 6, Inc Bartram Rd Bristol, PA USA

7 FUNCTIONALIZED SILICA-BASED PHASES REVERSE PHASE (HYDROPHOBIC) SORBENT SORBENT CODE STRURE C2 ethyl C02 -SiCH 2 CH 3 C3 propyl C03 -Si-(CH 2 ) 2 CH 3 C4 n-butyl Cn4 -Si-(CH 2 CH 3 Ci4 isobutyl Ci4 -Si-CH 2 CH(CH 3 ) 2 Ct4 tertiary butyl Ct4 -Si-C(CH 3 C5 pentyl C05 -Si-(CH 2 ) 4 CH 3 C6 hexyl C06 -Si-(CH 2 ) 5 CH 3 C7 heptyl C07 -Si-(CH 2 ) 6 CH 3 C8 octyl C08 -Si-(CH 2 ) 7 CH 3 C10 decyl C10 -Si-(CH 2 ) 9 CH 3 C12 dodecyl C12 -Si-(CH 2 ) 11 CH 3 C18 octadecyl C18 -Si-(CH 2 ) 17 CH 3 C20 eicosyl C20 -Si-(CH 2 ) 19 CH 3 C30 tricontyl C30 -Si-(CH 2 ) 29 CH 3 Cyclohexyl CYH1 -Si Phenyl PHY1 -Si NORMAL PHASE (HYDROPHILIC) Silica SIL1 -SiOH Diol DOL1 -Si-(CH 2 OCH 2 CHOHCH 2 OH Cyanopropyl CNP1 -Si-(CH 2 CN Florisil FLS Alumina, Acidic ALA Alumina, Neutral ALN Alumina, Basic ALB Carbon CARB ION EXCHANGE Anion pka Aminopropyl (1 amine) NAX1 -Si-(CH 2 NH N-2 Aminoethyl (1 & 2 amine) PSA1 -Si-(CH 2 NH(CH 2 ) 2 NH , 10.9 Diethylamino (3 amine) DAX1 -Si-(CH 2 N(CH 2 CH 3 ) Quaternary Amine Chloride QAX1 -Si-(CH 2 N + (CH 3 Cl- always charged Quaternary Amine Hydroxide CHQAX1 -Si-(CH 2 N + (CH 3 OH always charged Quaternary Amine Acetate CAQAX1 -Si-(CH 2 N + (CH 3 CH 3 COO always charged Quaternary Amine Formate CFQAX1 -Si-(CH 2 N + (CH 3 HCOO always charged Polyimine PAX -Si-(CH 2 -R-[NHCH 2 CH 2 ] X 7 Cation Carboxylic Acid CCX1 -Si-CH 2 COOH 4.8 Propylsulfonic Acid PCX1 -Si-(CH 2 SO 3 H <1 Benzenesulfonic Acid BCX1 -Si-(CH 2 ) 2 SO 3 H always charged Benzenesulfonic Acid High Load BCXHL1 -Si-(CH 2 ) 2 SO 3 H always charged Triacetic Acid TAX -Si-(CH 2 NH-(CH 2 ) 2 N(CH 2 COOH) 2 COPOLYMERIC (MULTIFUNCTIONAL PHASES) CH 2 COOH Aminopropyl + C8 NAX2 -Si-(CH 2 NH 2 & -Si-(CH 2 ) 7 CH 3 Quaternary Amine + C8 QAX2 -Si-(CH 2 N + (CH 3 & -Si-(CH 2 ) 7 CH 3 Carboxylic Acid + C8 CCX2 -Si-CH 2 COOH & -Si-(CH 2 ) 7 CH 3 Propylsulfonic Acid + C8 PCX2 -Si-(CH 2 SO 3 H & -Si-(CH 2 ) 7 CH 3 Benzenesulfonic Acid + C8 BCX2 -Si-(CH 2 ) 2 SO 3 H & -Si-(CH 2 ) 7 CH 3 Cyanopropyl + C8 CNP2 -Si-(CH 2 CN & -Si-(CH 2 ) 7 CH 3 Cyclohexyl + C8 CYH2 -Si & -Si-(CH 2 ) 7 CH 3 COVALENT PHASES Epoxy EPX -Si-(CH 2 - O - CH 2 - CH - CH 2 - CH 2 Aldehyde ALD -Si-(CH 2 ) 4 CHO Isocyanate ICN -Si-(CH, Inc. 2 NCO O 2731 Bartram Rd Bristol, PA USA Thiopropyl THX -Si-(CH 2 P SH F

8 FUNCTIONALIZED SILICA-BASED PHASES REVERSE PHASE (HYDROPHOBIC) SORBENT % ORGANIC EXCHANGE (meq/g) C2 ethyl 6.60 N/A C3 propyl 7.60 N/A C4 n-butyl 8.50 N/A Ci4 isobutyl 8.80 N/A Ct4 tertiary butyl 8.50 N/A C5 pentyl 9.50 N/A C6 hexyl N/A C7 heptyl N/A C8 octyl N/A C10 decyl N/A C12 dodecyl not tested N/A C18 octadecyl N/A C20 eicosyl N/A C30 tricontyl N/A Cyclohexyl N/A Phenyl N/A NORMAL PHASE (HYDROPHILIC) 8 Silica N/A N/A Diol 8.00 N/A Cyanopropyl 6.90 N/A Florisil N/A N/A Alumina, Acidic N/A N/A Alumina, Neutral N/A N/A Alumina, Basic N/A N/A Carbon N/A N/A ION EXCHANGE Anion Aminopropyl (1 amine) N-2 Aminoethyl (1 & 2 amine) Diethylamino (3 amine) Quaternary Amine Chloride Quaternary Amine Hydroxide Quaternary Amine Acetate Quaternary Amine Formate Polyimine Cation Carboxylic Acid Propylsulfonic Acid Benzenesulfonic Acid Benzenesulfonic Acid High Load Triacetic Acid 7.61 Anion 0.17 / Cation.06 COPOLYMERIC (MULTIFUNCTIONAL PHASES) Aminopropyl + C Quaternary Amine + C Carboxylic Acid + C Propylsulfonic Acid + C Benzenesulfonic Acid + C Cyanopropyl + C Cyclohexyl + C8 N/A N/A COVALENT PHASES Epoxy N/A N/A Aldehyde N/A N/A Isocyanate 7.1 N/A Thiopropyl 6.50 N/A, Inc Bartram Rd Bristol, PA USA

9 RESERVOIRS FOR BONDED PHASE EXTRACTIONS 9 Chemistries are offered on these particles sizes... Small Particle (5-20 μm) Intermediate Particle (25-40 μm) Standard Particle (40-63 μm) Large Particle ( μm) and are available in the following formats Stated Volume (ml) Tube Configuration Bed Diameter (mm) Sorbent Mass (mg) 1 Cylindrical Cylindrical Cylindrical Expanded Cylindrical Cylindrical Cylindrical Cylindrical , Inc Bartram Rd Bristol, PA USA

10 USE OF BONDED PHASES FOR SAMPLE PREPARATION 10 CONDITIONING, SOLVATION (WETTING) Columns are shipped dry, but those with hydrophobic character need to be solvated in order to interact efficiently and reproducibly with aqueous matrices. Sample capacity is severely reduced on a dry column. At low vacuum ( - 3 in. Hg) add 1.5 ml of methanol or acetonitrile per 100 mg of sorbent to the sample preparation column. Release the vacuum or begin flushing immediately upon completion. The more air which passes through the column before sample loading, the less solvated the sorbent will be. Apply deionized or distilled water to remove excess solvent which will interfere with hydrophobic binding. Use 1 ml H2O per 100 mg sorbent. Momentary high vacuum (5 to 8 in. Hg) may be necessary to restart flow. At in. Hg the column will resist air displacement (vacuum may be left on without drying the sorbent). If the sorbent is accidentally dried, resolvate and reflush. When using ion exchange columns, apply 1mL of buffer to the column after flushing to ensure that the sorbent ph is optimal for the sorbent analyte interaction desired. Where ion exchange interactions are involved, follow guidelines concerning pka, ph and ionic binding. Use the same vacuum guidelines as described for flushing. When doing work where a very clean baseline is required washing the column with the elution solvent as part of the conditioning step will improve the LOD and LOQ. SAMPLE PREPARATION AND APPLICATION Mechanisms may be hydrophobic, polar, or ionic. Add internal standard to the sample if quantitation is desired. Optimize sample application by removing particulates if necessary (centrifugation or filtering) and/or diluting viscous matrices with water or buffer to ensure proper ph for desired interactions. The analyte and sorbent should be uncharged for optimum hydrophobic retention. On ion exchange sorbents, analytes must be oppositely charged to the sorbent [anions (-) on anion exchange sorbents (+); cations (+) on cation exchange sorbents (-)]. During sample application, the analyte binds by displacing a counterion on the sorbent. Apply sample at a rate of 1mL/min. Again, a momentary increase in vacuum may be needed to initiate sample flow. WASHING THE SORBENT AND ELUTING Ideal washing removes as many interferences as possible while retaining the analyte(s). Ideal elution recovers 100% of the analyte while leaving behind interferences. Make certain your column is dry when changing between aqueous solutions and organic solvents. HYDROPHOBIC AND POLAR ANALYTES The best approach towards using these types of sorbents is to search for a solvent mixture which will wash the most interferences from the sorbent without loss of analyte. Note that wash ph may greatly affect cleanup and/or recovery. Keep analyte and sorbent pka in mind if applicable. Elute with the strongest organic solvent, or by raising the percentage of organic, possibly in combination with a ph change to disrupt binding. ION EXCHANGE Ionic bonds are strong enough to allow the analyte to remain bound while interferences are washed away with high percentages (up to 100%) of polar or nonpolar organic solvents. The ph will also affect sample cleanup. Remember to remain 2 ph units from the relevant pka of your analyte and sorbent, both of which need to remain charged for ionic retention. Elute with aqueous buffers containing a stronger counterion than your analyte (classic ion exchange) or by changing ph to disrupt the ionic attraction. Make sure the elution solvent has enough organic character to overcome any adsorption due to the packing material. COPOLYMERIC EXCHANGE For ionically bound analytes, use washes of high organic strength to remove interferences retained by hydrophobic (solvent strength dependent) interactions. If your analyte is also capable of hydrophobic binding, remove polar interferences ionically similar to your analyte by using aqueous or weak aqueous/organic washes while disrupting ionic (ph and ionic strength dependent) binding. Elute by simultaneously disrupting ionic and hydrophobic interactions, Inc Bartram Rd Bristol, PA USA

11 CLEAN SCREEN SAMPLE PREP PRODS 11, Inc Bartram Rd Bristol, PA USA

12 MECHANISM OF CLEAN SCREEN When a sample is loaded onto the column at ph 6, many carboxylic acid functionalities present in the sample are ionized. This creates a repulsion between the column and many sample borne interferences, thereby reducing the likelihood of their adsorbing onto the column. At this ph, ibuprofen & barbiturates are not ionized and are hydrophobically adsorbed on to the column (figure 1). At the same time, drugs with amine functionalities such as cocaine and phencyclidine adsorb on to the column via both hydrophobic and ionic attraction (figure 1). Sample Application NH + SO 3 C n H 2n+1 PCP IBUPROFEN CH C HO CH 3 O figure 1 12 The column can then be washed with water or weak aqueous buffers at or below ph 6 without risking loss of the analytes. After drying the column, it is possible to elute the hydrophobically bound analytes using solvents of minimal polarity such as methylene chloride or a hexane/ethyl acetate mixture (figure 2). Cationic analytes will remain bound to the column. Many compounds of intermediate polarity and potential interferences will also remain on the column. The majority of these potential interferences can be removed by using a methanol wash. Cationic analytes bound to the column can be eluted after another drying step. The drying steps are necessary to remove water which would have prevented the water immiscible elution solvents from optimally interacting with the analytes (figure 3). Elution 1 IBUPROFEN Dry Column SO 3 SO3 NH + C n H 2n+1 CH C HO CH 3 NH + O PCP PCP figure 2 CnH2n+1 H 2 O figure 3 To elute the cationic analytes, an organic solution with a high ph should be used. A methylene chlorideisopropanol-ammonium hydroxide mixture will simultaneously disrupt these ionic interactions and successfully elute the desired compound (figure 4). Elution 2 SO3 CnH2n+1 NH + PCP figure 4, Inc Bartram Rd Bristol, PA USA

13 CLEAN SCREEN COPOLYMERIC BONDED PHASES FOR DRUG ABUSE TESTING Analytical demand for more efficient, robust and clean extraction of drugs from biological matrices led to the development of CLEAN SCREEN sorbents. Since 1986, CLEAN SCREEN has led the industry with dependable and reproducible Solid Phase Extraction products and applications. CLEAN SCREEN phases are true copolymeric sorbents that contain hydrophobic and ion exchange functional groups uniquely polymerized to a silica substrate. The design and quality of CLEAN SCREEN provides superior sample clean up, recovery and reproducibility. Mixed mode separations allow maximum selectivity for extraction of acids, neutrals and bases. This selectivity makes CLEAN SCREEN ideal for both screening and confirmation analysis for virtually all drug categories. CLEAN SCREEN columns are used extensively by forensic and clinical chemists including: Post Mortem Investigations Criminal Investigations Urine Drug Testing Athletic Drug Testing Racing Laboratories Therapeutic Drug Monitoring Medical Drug Screening Note: If performing extractions out of viscous matrices such as tissue or horse urine, turn to our XtrackT section, where high-flow/gravity flow columns are found. The DAU CLEAN SCREEN sorbent as well as other phases are available in this larger particle size. DAU Part Number without Part Number with Sorbent Amount/ Unit per Clean-Thru Tips Clean-Thru Tips Tube Volume Pack CSDAU131 CCDAU mg/1mL 100 CSDAU133 CCDAU mg/3mL 50 CSDAU203 CCDAU mg/3mL 50 CSDAU303 CCDAU mg/3mL 50 CSDAU503 CCDAU mg/3mL 50 CSDAU206 CCDAU mg/6mL 50 CSDAU506 CCDAU mg/6mL 50 CSDAU1M6 CCDAU1M6 1g/6mL 30 ZSDAU005 ZCDAU005 50mg/10mL 50 ZSDAU013 ZCDAU mg/10mL 50 ZSDAU020 ZCDAU mg/10mL 50 CSDAU515 CCDAU mg/15mL 5 % Organic Loading: Exchange Capacity (meq/g): THC Part Number without Part Number with Sorbent Amount/ Unit per Clean-Thru Tips Clean-Thru Tips Tube Volume Pack CSTHC131 CCTHC mg/1mL 100 CSTHC203 CCTHC mg/3mL 50 CSTHC303 CCTHC mg/3mL 50 CSTHC503 CCTHC mg/3mL 50 CSTHC206 CCTHC mg/6mL 50 CSTHC506 CCTHC mg/6mL 50 CSTHC1M6 CCTHC1M6 1g/6mL 30 ZSTHC013 ZCTHC mg/10mL 50 ZSTHC020 ZCTHC mg/10mL 50 % Organic Loading: 12.3 Exchange Capacity (meq/g): GHB Part Number without Part Number with Unit per Clean-Thru Tips Clean-Thru Tips Pack CSGHB mg/3mL 50 ZSGHB mg/10mL 50 CLEAN SCREEN DAU column is copolymerized on a rigid, purified silica gel support. The two functional groups include a reverse phase, and an ion exchanger, benzenesulfonic acid. This column is commonly used for analyzing a wide range of drugs of abuse, including acidic, basic & neutral drugs. Dual functionality for weak bases and hydrophobic compounds. CLEAN SCREEN THC column is copolymerized on a rigid, purified silica gel support. The two functional groups include a reverse phase, and an ion exchanger, primary amine. This column is commonly usedfor analyzing THC and its metabolites. Dual functionality for acids and hydrophobic compounds. 13 The small polar nature of the molecule and the lack of a UV chromatophore complicate the chromatographic and spectrophotometricanalysis of GHB. Chemically, GHB is unstable and readily forms Gamma-butyrolactone when heated in, Inc Bartram Rd Bristol, PA USA acid conditions. Most analytical methods are based upon the interconversion P to the lactone and chemical F derivatization to form the TMS derivative. This column is for the extraction of free GHB.

14 CLEAN SCREEN XCEL TM 14 w w w. u n i t e d c h e m. c o m 2 STEP EXTRACTION COLUMN, Inc Bartram Rd Bristol, PA USA

15 CLEAN SCREEN XCEL SOLID PHASE EXTRACTION COLUMNS The Clean Screen Xcel solid phase extraction columns are a line of sample prep phases designed to reduce the number of steps in the extraction, and therefore the amount of time and solvent necessary to complete the sample cleanup. Further advantages include reduced sample size and improved cleanliness and recovery. CLEAN SCREEN XCEL I: The Xcel I column will extract a wide array of basic drugs including benzodiazepines and opiates. CLEAN SCREEN XCEL I Endcapped Tube Volume Pack CSXCE mg/1mL 100 CSXCE mg/3mL 50 CSXCE mg/6mL 50 ZSXCE mg/10mL 50 CLEAN SCREEN XCEL II: The Xcel II column is designed solely for the extraction of the THC metabolite. CLEAN SCREEN XCEL II Endcapped Tube Volume Pack CSXCE mg/1mL 100 CSXCE mg/3mL 50 CSXCE mg/6mL 50 ZSXCE mg/10mL 50 CLEAN SCREEN XCEL SOLID PHASE EXTRACTION WELL PLATES DEEP WELL PLATES Tube Volume Pack WSH96XCE11 130mg 1 48 DEEP WELL PLATES Tube Volume Pack WSH48XCE11 130mg 1, Inc Bartram Rd Bristol, PA USA

16 CLEAN SCREEN FASt SOLID PHASE EXTRACTION COLUMNS A FASTER AND CLEANER SPE ALTERNATIVE TO DILUTE AND SHOOT CLEAN SCREEN FASt employs a process that uses positive pressure and a solid phase sorbent bed built with small pore frits to quickly and efficiently prepare samples for LC/MS analysis. This method eliminates the timely centrifugation, reduces matrix suppression effects and removes particulates greater than ~ 1µm. Samples can be diluted at a ratio as low as 1:1, which is `useful for analytes at very low concentrations. Benefits: Eliminate the centrifuge and sample transfer steps Lower costs by decreasing turn-around time Reducing instrument and LC column maintenance CLEAN SCREEN FASt 16 Endcapped Tube Volume Pack CSFAS mg/3mL 50 ZSFAS mg/10mL DEEP WELL PLATE Tube Volume Pack WSH96FAS11-10LD 100mg 1 CLEAN SCREEN FASt THC Endcapped Tube Volume Pack CSFASTH mg/3mL 50 ZSFASTH mg/10mL 50 CLEAN SCREEN FASt THC SOLID PHASE EXTRACTION WELL PLATE 96 DEEP WELL PLATE Tube Volume Pack WSH96FASTH11-10LD 100mg 1, Inc Bartram Rd Bristol, PA USA

17 CLEAN SCREEN ETHYL GLUCURONIDE SOLID PHASE EXTRACTION COLUMN BENZODIAZEPINE SOLID PHASE EXTRACTION COLUMN ETG Part Number without Part Number with Sorbent Amount/ Unit per Clean-Thru Tips Clean-Thru Tips Tube Volume Pack CSETG203 CCETG mg/3mL 50 ZSETG040 ZCETG mg/10mL 50 CLEAN SCREEN ETG columns are available exclusively from for analysis by GC/MS & LC/MS. This 3mL column contains a proprietary carbon packing material for the extraction and concentration of ethyl glucuronide. BNZ Part Number without Part Number with Sorbent Amount/ Unit per Clean-Thru Tips Clean-Thru Tips Tube Volume Pack CSBNZ203 CCBNZ mg/3mL 50 CSBNZ mg/6mL 50 ZSBNZ mg/10mL 50 ZSBNZ mg/10mL 50 CLEAN SCREEN BNZ columns are available for benzodiazepine extractions, with specific focus on 7-amino benzodiazepines. 17 CLEAN-THRU TIPS CLEAN-THRU tips provides the first solid phase extraction cartridge system that eliminates sample carry over from the vacuum manifold lid. The technology was pioneered by our research scientists & consists of one of our SPE columns with a disposable tip that attaches to the end of each column. Columns available in the CLEAN-THRU configuration are found in the CLEAN SCREEN sections. This system was designed in order to meet the strict requirements that the Substance Abuse and Mental Health Services Administration certification has placed on laboratories to address the problem of cross contamination between samples. CLEANTHRU tips provide a completely disposable system that eliminates any contact between the sample, wash solvents and the extraction apparatus. The continuous passage of the sample through the system provides a direct, accurate route to waste or collection vessels. As each extraction is completed, the column and tip are discarded as a unit. CLEAN-THRU eliminates your concerns about sample residue remaining in the extraction system. CLEAN SCREEN sorbent as well as other phases are available in this larger particle size. CLEAN-THRU TIPS Part Number Description Unit per bag CLTTP050 TIP 50 Standard Configuration s Column with a CLEAN-THRU Tip, Inc Bartram Rd Bristol, PA USA

18 CLEAN SCREEN RSV REDUCED SOLVENT VOLUME EXTRACTION COLUMNS Reduced Solvent Volume extraction columns are micro bed packed columns which offer the advantages of disc technology yet maintain the proven track record of our conventional SPE columns. Reduced Solvent Volume columns use 75% less solvent than traditional packed columns. Less solvent means faster extractions, higher throughput and less waste disposal, which translates into significant savings in both time and money. Results demonstrate that therapeutic and abused drugs in urine and blood matrices can be extracted with cleanliness, high recoveries and consistent reproducibility by using the Reduced Solvent Volume extraction column. 18 Advantages of CLEAN SCREEN RSV: 75% Reduction in total liquid volumes Lower cost per extraction Faster extraction times Less disposal cost Increased automated throughput 50% Reduction in eluate volume Faster dry down times Reduced exposure to organic solvents Superior flow characteristics Less flow restriction from matrix proteins or particulates More reliable for automated processing High capacity Greater linear range, Inc Bartram Rd Bristol, PA USA

19 CLEAN SCREEN RSV REDUCED SOLVENT VOLUME EXTRACTION COLUMNS CAPACITY Absolute Recovery % (n=4) for 1000 ng/ml 90% (n=2) for 3000 ng/ml 91% (n=4) for 4000 ng/ml No analyte breakthrough at 1000 ng/ml and less than 0.2% analyte breakthrough at 3000 and 4000 ng/ml levels. THC-COOH Standards (ng/ml) in spiked urine RECOVERY 19 Concentration (ng/ml) ng/mL 2ng/mL Linear Ranges Exhibiting Greater than 85% Recoveries in Urine Highest Concentration Tested Lowest Concentration Tested Butalbital Amobarbital Pentobarbital Secobarbital Phenobarbital Benzoylecgonine Phencyclidine THC-COOH Analyte SOLVENT REDION Analyte Solvent Usage Solvent Usage % Solvent Clean-Thru Tips Reduced Solvent Traditional Packed Reduction Volume SPE columns columns Barbiturates 4.25 ml 18 ml 76% Benzoylecgonine 4.65 ml 19 ml 76% THC-COOH 4.85 ml 16.4 ml 73% Phencyclidine 5.15 ml 19 ml 70%, Inc Bartram Rd Bristol, PA USA

20 CLEAN SCREEN RSV REDUCED SOLVENT VOLUME EXTRACTION COLUMNS COPOLYMERIC EXTRACTION FOR ALL DRUG CLASSES Part Number without Part Number with Sorbent Amount/ Unit per Clean-Thru Tips Clean-Thru Tips Tube Volume Pack CSDAUA83 CCDAUA83 80mg/3mL 50 ZSDAUA08 ZCDAUA08 80mg/10mL 50 CLEAN SCREEN DAU Column is copolymerized on a rigid, purified silica gel support. The two functional groups include a reverse phase, and an ion exchanger, benzenesulfonic acid. This column is commonly used for analyzing a wide range of drugs of abuse, including acidic, basic and neutral drugs. SPECIFICALLY FOR THC & THCA EXTRACTION 20 Part Number without Part Number with Sorbent Amount/ Unit per Clean-Thru Tips Clean-Thru Tips Tube Volume Pack CSTHCA83 CCTHCA83 80mg/3mL 50 ZSTHCA08 ZCTHCA08 80mg/10mL 50 CLEAN UP RSV REDUCED SOLVENT VOLUME EXTRACTION COLUMNS CLEAN SCREEN THC Column is copolymerized on a rigid, purified silica gel support. The two functional groups include a reverse phase, and an ion exchanger, quaternary amine. This column is commonly used for analyzing THC and its metabolites. C18, OCTADECYL TRI-FUNCTIONAL Part Number Pack Endcapped Unendcapped Tube Volume Pack CEC18A083 CUC18A083 80mg/3mL 50 % Organic Loading: Removes hydrophobic impurities, de-salting and purification of hydrophobic compounds., Inc Bartram Rd Bristol, PA USA

21 XtrackT HIGH-FLOW SOLID PHASE EXTRACTION COLUMNS 21, Inc Bartram Rd Bristol, PA USA

22 XtrackT HIGH-FLOW BONDED PHASES Advantages of XtrackT Resists plugging Improved flow of all sample types Gravity flow of most samples Broad spectrum extractions Very clean extractions High recoveries Reproducibility Viscous sample matrices are frequently resistant to flow through standard solid phase columns. Increased particle size enhances flow characteristics allowing ease of sample application and analysis. XtrackT columns are designed to give uniform flow for even the most viscous samples including equine urine, post mortem blood and tissues, meconium, amniotic fluid, milk, etc. XtrackT also functions as a gravity flow column for most blood and urine samples. A single column provides extraction for a broad spectrum of compounds with selective elution of acid neutrals, steroids and bases XtrackT yields very clean extractions and excellent recoveries without need for additional liquid clean up steps. XtrackT is available in hydrophobic, hydrophilic, ion exchange, and copolymeric phases, including the CLEAN SCREEN DAU sorbent. XtrackT is recommended for any chemist challenged by viscous sample matrices, or those desiring gravity flow capacity. Upon request, we can also provide any of our CLEAN-UP sorbents in this large particle size. 22 GRAVITY-FLOW CLEAN SCREEN DAU COLUMNS Part Number with Part Number without Sorbent Amount/ Unit per Clean-Thru Tips Clean-Thru Tips Tube Volume Pack XRDAH203 XCDAH mg/3mL 50 XRDAH303 XCDAH mg/3mL 50 XRDAH503 XCDAH mg/3mL 50 XRDAH206 XCDAH g/6mL 50 XRDAH506 XCDAH g/6mL 50 XRDAH20Z XCDAH20Z 200mg/10mL 50 XRDAH50Z XCDAH50Z 500mg/10mL 50 XRDAH515 XCDAH mg/15mL 50 XRDAHM15 XCDAHM15 1g/15mL 30 CLEAN SCREEN DAU Column is copolymerized on a rigid, purified silica gel support. The two functional groups include a reverse phase, and an ion exchanger, benzenesulfonic acid. This column is commonly used for analyzing a wide range of drugs of abuse, including acidic, basic & neutral drugs. HIGH-FLOW CLEAN-UP ENDCAPPED C18 COLUMNS Part Number with Part Number without Sorbent Amount/ Unit per Clean-Thru Tips Clean-Thru Tips Tube Volume Pack XRODHM06 XCODHM06 1g/6mL 30 XRODH515 XCODH mg/15mL 50 % Organic Loading: Removes hydrophobic impurities, de-salting and purification of hydrophobic compounds. HIGH-FLOW CLEAN-UP PROPYLSULFONIC ACID COLUMNS Part Number with Part Number without Sorbent Amount/ Unit per Clean-Thru Tips Clean-Thru Tips Tube Volume Pack XRPCH50Z XCPCH50Z 500mg/10mL 50 % Organic Loading: 9.70 Scavenger for amines, alcohols and other nucleophiles High-Flow also available in: Carboxylic Acid, Aminoethyl, Benzenesulfonic Acid and Quaternary Amine, Inc Bartram Rd Bristol, PA USA

23 CLEAN-UP SOLID PHASE EXTRACTION COLUMNS 23 w w w. u n i t e d c h e m. c o m HYDROPHOBIC HYDROPHILIC ION EXCHANGE, Inc Bartram Rd Bristol, PA USA

24 CLEAN-UP HYDROPHOBIC EXTRACTION COLUMNS 24 This sorbent is composed of a silica backbone bonded with hydrocarbon chains. It is used to extract compounds which exhibit non-polar or neutral characteristics out of complex matrices. The C18 phase is the most widely used for non-polar interactions because of its nonselective nature; C18 will extract a large number of compounds with differing chemical properties. To enchance selectivity, offers a wide range of hydrophobic sorbents. Multiple chain configurations are available for some Endcapped or unendcapped sorbents are available for all chain lengths. Example of a Hydrophobic Phase Si O OSi(CH 3 Si O Si (CH 2 ) 17 CH 3 O OSi(CH 3 Si O OSi(CH 3 Si O Si (CH 2 ) 17 CH 3 O OSi(CH 3 Silica Backbone Hydrocarbon Chain Analytes* Washes Elutions alkanes aqueous, non-polar alkenes usually with to aromatics some polar polar organic neutral compounds organic solvent *typical compounds which can be extracted using hydrophobic columns, Inc Bartram Rd Bristol, PA USA

25 CLEAN-UP HYDROPHOBIC EXTRACTION COLUMNS MECHANISM OF HYDROPHOBIC BONDING Compounds are retained by non-polar interactions from polar solvents or matrix environments. They are bound by dispersion forces / van der Waals forces. Elution, or disruption of the non-polar interactions is achieved by solvents or solvent mixtures with sufficient non-polar character. Some polar solvents, such as acetonitrile have enough non-polar characteristics to disrupt nonpolar binding to cause elution of a compound from the sorbent. Methanol can be used as well, although it should be noted that it will take off both polar and nonpolar analytes of interest and interferences. Hydrophobic Sorbents & Structures Sorbent Structure C2 ethyl -SiCH 2 CH 3 C3 propyl -Si-(CH 2 ) 2 CH 3 C4 n-butyl -Si-(CH 2 CH 3 Ci4 isobutyl -Si-CH 2 CH(CH 3 ) 2 Ct4 tertiary butyl -Si-C(CH 3 C5 pentyl -Si-(CH 2 ) 4 CH 3 C6 hexyl -Si-(CH 2 ) 5 CH 3 Example of a Hydrophobic Bonding C7 heptyl -Si-(CH 2 ) 6 CH 3 C8 octyl -Si-(CH 2 ) 7 CH 3 H H H C10 decyl -Si-(CH 2 ) 9 CH 3 25 Sorbent C H H C H C H H C H C H H C H Compound C12 dodecyl -Si-(CH 2 ) 11 CH 3 C18 octadecyl -Si-(CH 2 ) 17 CH 3 C20 eicosyl -Si-(CH 2 ) 19 CH 3 C30 tricontyl -Si-(CH 2 ) 29 CH 3 Cyclohexyl -Si Phenyl -Si UNENDCAPPED VS. ENDCAPPED Bonded phases are manufactured by the reaction of organosilanes with activated silica. During the polymerization reaction of carbon chains to the silica backbone, a very stable silyl ether linkage forms. Our unendcapped columns allow hydroxyl sites to remain, thus making these columns slightly hydrophilic. In order to decrease this slight polarity, these hydroxyl sites are deactivated. Proprietary bonding techniques ensure that these sites are 100% reacted, leading to a complete endcapping. Because there are no hydroxyl sites left, our endcapped columns are more hydrophobic than our unendcapped columns. O OH O OSi(CH 3 Si Si O O Si OH Unendcapped Structure Si Si O O Si OSi(CH 3 Endcapped Structure, Inc Bartram Rd Bristol, PA USA

26 CLEAN-UP HYDROPHOBIC EXTRACTION COLUMNS C8, OCTYL Part Number Endcapped Unendcapped Tube Volume Pack CEC081L1 CUC081L1 50mg/1mL 100 CEC08111 CUC mg/1mL 100 CEC08113 CUC mg/3mL 50 CEC08123 CUC mg/3mL 50 CEC08153 CUC mg/3mL 50 CEC08156 CUC mg/6mL 50 CEC081M6 CUC081M6 1g/6mL 30 CEC0811Z CUC0811Z 100mg/10mL 50 CEC0812Z CUC0812Z 200mg/10mL 50 CEC0812M15 CUC0812M15 2g/15mL 20 CEC0815M25 CUC0815M25 5g/25mL 20 % Organic Loading: Removes large or more hydrophobic compounds. 26 C18, OCTADECYL Part Number Endcapped Unendcapped Tube Volume Pack CEC181L1 CUC181L1 50mg/1ml 100 CEC18111 CUC mg/1ml 100 CEC18113 CUC mg/3ml 50 CEC18123 CUC mg/3ml 50 CEC18153 CUC mg/3ml 50 CEC18156 CUC mg/6ml 50 CEC181M6 CUC181M6 1g/6ml 30 CEC1811Z CUC1811Z 100mg/10ml 50 CEC1812Z CUC1812Z 200mg/10ml 50 CEC1815Z CUC1815Z 500mg/10ml 50 CEC1812M15 CUC1812M15 2g/15ml 20 CEC1815M25 CUC1815M25 5g/25ml 20 CEC18110M375 CUC18110M75 10g/75ml 10 % Organic Loading: 8.50 Removes large or more hydrophobic compounds. C30, TRICONTYL Part Number Endcapped Unendcapped Tube Volume Pack CEC30111 CUC mg/1mL 100 CEC30123 CUC mg/3mL 50 CEC30156 CUC mg/6mL 50 CEC301M6 CUC301M6 1g/6mL 30 % Organic Loading: Removes large or more hydrophobic compounds., Inc Bartram Rd Bristol, PA USA

27 CLEAN-UP HYDROPHOBIC EXTRACTION COLUMNS CYCLOHEXYL Part Number Endcapped Unendcapped Tube Volume Pack CECYH123 CUCYH mg/3mL 50 CECYH153 CUCYH mg/3mL 50 CECYH1M6 CUCYH1M6 1g/6mL 30 CECYH12M15 CUCYH12M15 2g/15mL 20 % Organic Loading: Scavenger for phenolic compounds. PHENYL Part Number Endcapped Unendcapped Tube Volume Pack CEPHY1L1 CUPHY1L1 50mg/1mL 100 CEPHY111 CUPHY mg/1mL 100 CEPHY123 CUPHY mg/3mL 50 CEPHY153 CUPHY mg/3mL 50 CEPHY156 CUPHY mg/6mL 50 CEPHY1M6 CUPHY1M6 1g/6mL 30 CEPHY12Z CUPHY12Z 200mg/10mL 50 % Organic Loading: 11.0 Scavenger for polar compounds. 27 OTHER REVERSE PHASES AVAILABLE: C2 ethyl C3 propyl C4 n-butyl Ci4 isobutyl Ct4 tertiary butyl C5 pentyl C6 hexyl C7 heptyl C10 decyl C12 dodecyl C20 eicosyl, Inc Bartram Rd Bristol, PA USA

28 CLEAN-UP 28 This sorbent is composed of a CT HYDROPHILIC NORMAL PHASE EXTRACTION COLUMNS silica backbone bonded with carbon chains containing polar functional groups. Groups which will possess such polarity include amines, hydroxyls and carbonyls. Si Si Si Si Example of a Hydrophilic Phase O OH OH OH O Si (CH 2 O CH 2 CH CH2 O OH O OH OH OH O Si (CH 2 O CH 2 CH CH2 O OH Silica Backbone Hydrophilic Chain Analytes* Washes Elutions R-OH, R-SH non-polar organic polar organic R-NH2, solvents solvent R2-NH, ie: hexane/ethyl acetate (80:20) usually with R3-N methylene chloride some aqueous, Inc Bartram Rd Bristol, PA USA

29 CLEAN-UP HYDROPHILIC NORMAL PHASE EXTRACTION COLUMNS Mechanism of Hydrophilic Bonding Compounds are retained on hydrophilic sorbents through polar interactions including hydrogen bonding, pi-pi or dipole-dipole interaction. These types of interactions occur when a distribution of electrons between individual atoms in functional groups is unequal, causing negative and positive polarity. Compounds typically extracted on a hydrophilic column include analytes which have polar groups, including amines, hydroxyls and carbonyls. Elution is performed by strong polar solvents. Sorbent Silica Diol Cyanopropyl Hydrophilic Sorbents & Structures Structure -SiOH -Si-(CH 2 OCH 3 CHOHCH 2 OH -Si-(CH 2 CN Note: If un-ionized, ion exchange sorbents can be used as hydrophilic (polar) sorbents. 29 Example of Hydrophilic Bonding OH CH Sorbent CH 2 O - O + H - O C CH 2 CH 2 H + Compound, Inc Bartram Rd Bristol, PA USA

30 CLEAN-UP HYDROPHILIC EXTRACTION COLUMNS UNBONDED SILICA (ACID WASHED) Endcapped Tube Volume Pack CUSIL mg/1mL 100 CUSIL mg/3mL 50 CUSIL mg/3mL 50 CUSIL mg/6mL 50 CUSIL1M6 1g/6mL 30 CUSIL11Z 100mg/10mL 50 CUSIL12M15 2g/15mL 20 CUSIL15M25 5g/25mL 20 CUSIL110M75 10g/75mL μm Particle Size 60A Pore Size 500m 2 /g Surface Area Removes hydrophilic (polar) impurities, purification of hydrophilic (polar) compounds. 30 PHARMA-SIL Endcapped Tube Volume Pack PHSIL1L1 50mg/1mL 100 PHSIL mg/3mL 50 PHSIL mg/6mL 50 PHSIL1M6 1g/6mL 30 PHSIL12M15 2g/15mL 20 PHSIL15M25 5g/25mL 20 HIGH SURFACE ACTIVITY SILICA Endcapped Tube Volume Pack HSSIL mg/3mL 50 HSSIL mg/6mL μm Particle Size 60A Pore Size 525m 2 /g Surface Area Removes hydrophilic (polar) impurities, purification of hydrophilic (polar) compounds μm Particle Size 60A Pore Size 547m 2 /g Surface Area Removes hydrophilic (polar) impurities, purification of hydrophilic (polar) compounds. FLORISIL Endcapped Tube Volume Pack CUFLS mg/1mL 100 CUFLS mg/3mL 50 CUFLS mg/3mL 50 CUFLS mg/6mL 50 CUFLS1M6 1g/6mL 30 CUFLS11Z 100mg/10mL 50 CUFLS12Z 200mg/10mL 50 CUFLS15Z 500mg/10mL 50 CUFLS12M15 2g/15mL 20 CUFLS15M25 5g/25mL 20 CUFLS110M75 10g/75mL 10 % Organic Loading: N/A Removes polar-type compounds. Florisil products are manufactured by U.S. Silica, Co., Inc Bartram Rd Bristol, PA USA

31 CLEAN-UP HYDROPHILIC EXTRACTION COLUMNS ALUMINA, ACIDIC Endcapped Tube Volume Pack CUALA mg/1mL 100 CUALA mg/3mL 50 CUALA mg/3mL 50 CUALA mg/6mL 50 CUALA1M6 1g/6mL 30 CUALA12M15 2g/15mL 20 % Organic Loading: N/A Removes polar-type compounds. ALUMINA, BASIC 31 Endcapped Tube Volume Pack CUALB mg/3mL 50 CUALB mg/3mL 50 CUALB mg/6mL 50 CUALB1M6 1g/6mL 30 CUALB12Z 200mg/10mL 50 CUALB15Z 500mg/10mL 50 CUALB12M15 2g/15mL 20 CUALB15M25 5g/25mL 20 CUALB110M75 10g/75mL 10 % Organic Loading: N/A Removes polar-type compounds. ALUMINA, NEUTRAL Endcapped Tube Volume Pack CUALN1L1 50mg/1mL 100 CUALN mg/1mL 100 CUALN mg/3mL 50 CUALN mg/3mL 50 CUALN mg/6mL 50 CUALN1M6 1g/6mL 30 CUALN12Z 200mg/10mL 50 CUALN15Z 500mg/10mL 50 CUALN12M15 2g/15mL 20 CUALN15M25 5g/25mL 20 % Organic Loading: N/A Removes polar-type compounds., Inc Bartram Rd Bristol, PA USA

32 CLEAN-UP HYDROPHILIC EXTRACTION COLUMNS CN, CYANOPROPYL Part Number Endcapped Unendcapped Tube Volume Pack CECNP1L1 CUCNP1L1 50mg/1mL 100 CECNP111 CUCNP mg/1mL 100 CECNP123 CUCNP mg/3mL 50 CECNP153 CUCNP mg/3mL 50 CECNP156 CUCNP mg/6mL 50 CECNP1M6 CUCNP1M6 1g/6mL 30 CECNP12M15 CUCNP12M15 2g/15mL 20 CECNP110M75 CUCNP110M75 10g/75mL 10 % Organic Loading: 6.90 Removes steroid-type compounds. 32 DIOL Endcapped Tube Volume Pack CUDOL mg/1mL 100 CUDOL mg/3mL 50 CUDOL mg/3mL 50 CUDOL mg/6mL 50 CUDOL15Z 500mg/10mL 50 CUDOL12M15 2g/15mL 20 % Organic Loading: 8.00 Removes hydrophilic (polar) impurities, purification of hydrophilic (polar) compounds. CARBON-GRAPHITIZED NON-POROUS, 120/400 MESH Endcapped Tube Volume Pack CUCARB1L1 50mg/1mL 100 CUCARB mg/1mL 100 CUCARB mg/3mL 50 CUCARB mg/3mL 50 CUCARB mg/6mL 50 CUCARB1M6 1g/6mL 30 CLEAN-UP Carbon Carbon supports have been used to isolate extremely polar organic compounds. They work by a hydrophobic mechanism with a high surface area and ion exchange. This interaction can happen in a wide range of polar and non-polar solvents., Inc Bartram Rd Bristol, PA USA

33 CT This sorbent is composed of a silica backbone bonded with a carbon chain terminated by a negatively or positively charged functional group. Ion exchange interactions occur between a sorbent that carries a charge and a compound of opposite charge. Si O OH Si O Si (CH 2 ) 2 O OH Si O OH Si Example of a Cation Exchange Phase O Si (CH 2 ) O OH CLEAN-UP 2 Silica Backbone Anion Exchanger SO 3 SO 3 ION EXCHANGE EXTRACTION COLUMNS Example of a Anion Exchange Phase Si O OH Si O Si (CH 2 NH + 3 O OH Si Si O O OH Si (CH 2 NH + 3 O OH Silica Backbone Anion Exchanger This electrostatic interaction is reversible by neutralizing the sorbent and /or analyte. Ion exchange bonds can also be disrupted by introduction of a counter ion to compete with the analyte for binding sites on the sorbent. 33 Anions Cations Organic solvent or aqueous buffer at ph that allows the ion to remain charged AND/OR at a low ionic strength AND/OR at a weak concentration. Organic solvent or aqueous buffer at ph that would neutralize the ion AND/OR at a high ionic strength AND/OR at a strong concentration., Inc Bartram Rd Bristol, PA USA

34 H 3 + N Compound MECHANISM OF ION EXCHANGE BONDING Compounds are retained on the sorbent through ionic bonds. Therefore, it is essential that the sorbent and the analyte to be extracted are charged. Generally, the number of molecules with charged cationic groups increases at ph values below the molecules pka value. The number of molecules with charged anionic groups decreases at ph values below the molecule s pka value. To ensure 99% or more ionization, the ph should be at least two ph units below the pka of the cation and two ph units above the pka of the anion. Elution occurs by using a solvent to raise the ph above the pka of the cationic group or to lower the ph below the pka of the anion to disrupt retention. At this point, the sorbent or compound will be neutralized. ION EXCHANGE SORBENTS & STRURE Sorbent Structure pka 34 Anion Exchangers Aminopropyl (1º amine) + -Si-(CH 2 NH N-2 Aminoethyl (1º & 2º amine) + -Si-(CH 2 NH 2+ (CH 2 ) 2 NH , 10.9 Diethylamino (3º amine) -Si-(CH 2 NH + (CH 2 CH 3 ) Quaternary Amine Chloride -Si-(CH 2 N + (CH 3 Cl - always charged Quaternary Amine Hydroxide -Si-(CH 2 N + (CH 3 OH always charged Quaternary Amine Acetate -Si-(CH 2 N + (CH 3 CH 3 COO always charged Quaternary Amine Formate -Si-(CH 2 N + (CH 3 HCOO always charged Polyimine -Si-(CH 2 R - [NHCH 2 CH 2 ] X Cation Exchangers Carboxylic Acid -Si-CH 2 COOH 4.8 Propylsulfonic Acid -Si-(CH 2 SO 3 H <1 Benzenesulfonic Acid -Si-(CH 2 ) 2 SO 3 H always charged Benzenesulfonic Acid High-Load -Si-(CH 2 ) 2 SO 3 H always charged Triacetic Acid -Si-(CH 2 NH-(CH 2 )-N(CH 2 COOH) 2 CH 2 COOH NOTE: In an un-ionized state, these sorbents are hydrophilic (polar) sorbents. NOTE: Neutralization can occur on either the sorbent or the analyte of interest. Either will disrupt the bond of the desired compound. Sorbent CH 2 NH O C O Compound Example of Anion Exchange Binding Example of Cation Exchange Binding SO 3 - Sorbent, Inc Bartram Rd Bristol, PA USA

35 CLEAN-UP ANION EXTRACTION COLUMNS AMINOPROPYL Tube Volume Pack CUNAX1L1 50mg/1mL 100 CUNAX mg/1mL 100 CUNAX mg/3mL 50 CUNAX mg/3mL 50 CUNAX mg/6mL 50 CUNAX1M6 1g/6mL 30 CUNAX11Z 100mg/10mL 50 CUNAX12Z 200mg/10mL 50 CUNAX15Z 500mg/10mL 50 CUNAX12M15 2g/15mL 20 CUNAX15M25 5g/25mL 20 CUNAX110M75 10g/75mL 10 % Organic Loading: 6.65 Exchange Capacity (meq/g): Scavenger for acids, cyclic compounds, cholesterols, and other lipid-type and compounds. PSA ( N-2 AMINOETHYL ) Tube Volume Pack CUPSA1L1 50mg/1mL 100 CUPSA mg/1mL 100 CUPSA mg/3mL 50 CUPSA mg/3mL 50 CUPSA mg/6mL 50 CUPSA1M6 1g/6mL 30 CUPSA11Z 100mg/10mL 50 CUPSA110M75 10g/75mL 10 % Organic Loading: 9.70 Exchange Capacity (meq/g): Scavenger for acids, cyclic compounds, cholesterols, and other lipid-type and compounds. 35 DIETHYLAMINO Tube Volume Pack CUDAX mg/1mL 100 CUDAX mg/3mL 50 CUDAX mg/3mL 50 CUDAX mg/6mL 50 CUDAX12M15 2g/15mL 20 CUDAX15M25 5g/25mL 20 % Organic Loading: 9.70 Exchange Capacity (meq/g): Scavenger for acids, cyclic compounds, cholesterols, and other lipid-type and compounds., Inc Bartram Rd Bristol, PA USA

36 A MAP TO OPTIMIZING SAMPLE CLEAN UP SAMPLE NATURE OF SAMPLE GAS LIQUID SOLID ANALYTE CONFIGURATION MOLECULAR NATURE OF ANALYTE HYDROPHOBIC, LOG P > 2.5 HYDROPHILIC, LOG P < 2.5 MATRIX AQUEOUS, BIOLOGICAL FLUIDS, BUFFERS NON POLAR ORGANIC 36 TYPE OF EXTRACTION NON POLAR, REVERSE PHASE EXTRACTION POLAR OR NORMAL PHASE EXTRACTION BONDING MECHANISM TYPES OF FUNCTIONAL GROUPS INVOLVES VANDERWALLS/DISPERSION FORCES INTERACTION BETWEEN SORBENT CH BONDS AND ANALYTE CH BONDS PROTONATED/NEUTRAL STATE, AROMATICS, ALKYLCHAINS INVOLVES HYDROGEN BONDING π π AND DIPOLE/DIPOLE INTERACTIONS AMINES, HYDROXYLS, CARBONYLS, AROMATIC RINGS, HETERO ATOMS (O, S, N, P) ANALYTE POLARITY LOG P > 3 LOG P < 2 ANALYTE CHARGE δ+ LOG P < 1 δ- SORBENT TYPE C 8 - C 30 PS/DVD C 2 - C 7 CYCLOHEXYL CN, NH 2, PSA, PHENYL, PI CARBON ALUMINA SILICA GEL FLUOROSIL DIOL NON POLAR TO MODERATELY POLAR SOLVENTS WATER/ACETONITRILE/METHANOL MIXES ELUTION SOLVENT: MEDIUM TO HIGH POLARITY SOLVENTS METHANOL/ETHYL ACETATE/ACETONE/THF MIXES LIPIDS, OIL ADDITIVES, CARBOHYDRATES, PHENOLS, OIL SOLUBLE VITAMINS, PESTICIDES, HERBICIDES TYPICAL APPLICATIONS: DRUGS OF ABUSE, PHARMACEUTICALS, TDM, PESTICIDES PARTITION COEFFICIENT P o/w = (C oil /C water) EQUILIBRIUM. OIL IS USUALLY OCTANOL LOG P SCALE HYDROPHILIC (POLAR) < 1, Inc Bartram Rd Bristol, PA USA P MODERATELY POLAR F HYDROPHOBIC (NONPOLAR) > 2

37 QUATERNARY AMINE WITH CHLORIDE COUNTER ION CLEAN-UP ANION EXTRACTION COLUMNS USING SOLID PHASE EXTRACTION Tube Volume Pack CUQAX1L1 50mg/1mL 100 CUQAX mg/1mL 100 CUQAX mg/3mL 50 CUQAX mg/3mL 50 CUQAX mg/6mL 50 CUQAX1M6 1g/6mL 30 IONIC CUQAX12Z 200mg/10mL 50 CUQAX15Z 500mg/10mL 50 CUQAX12M15 2g/15mL 20 MUST BE ABLE TO BE CHARGED % Organic Loading: 8.40 Exchange Capacity (meq/g): NATURE OF SAMPLE Scavenger for acids and sulfonyl chlorides, isocyanates and weak ANALYTE electrophiles. CONFIGURATION NATURE OF ANALYTE AQUEOUS OR ORGANIC QUATERNARY AMINE WITH ACETATE COUNTER ION Part Number Sorbent Amount/ ION Unit EXCHANGE per Tube Volume Pack CAQAX mg/1mL 100 CAQAX mg/3mL 50 CAQAX1M6 1g/6mL 30 CAQAX15M25 5g/25mL INVOLVES 20IONIC EXCHANGE QUATERNARY AMINE WITH HYDROXIDE COUNTER ACIDS AND ION BASES ANIONS: PHOSPHATES, CARBOXYLIC ACIDS, Part Number Sorbent Amount/ SULFONIC Unit per ACIDS Tube Volume CATIONS: AMINES, Pack PURINES, PYRIMIDINES CHQAX1L1 50mg/1mL 100 CHQAX mg/1mL 100 CHQAX mg/3mL ANIONS 50 CATIONS CHQAX mg/3mL 50 CHQAX mg/6mL 50 CHQAX1M6 WEAK 1g/6mL STRONG 30 STRONG CHQAX12Z 200mg/10mL 50 QAX & NH 2, DEAE, CCX, TAX, & CHQAX12M15 2g/15mL 20 COPOLYMERS PSA, PI, & COPOLYMERS CHQAX15M25 5g/25mL 20 COPOLYMERS WEAK BCX, PCX & COPOLYMERS MATRIX TYPE % Organic Loading: 8.40 OF EXTRACTION Exchange Capacity (meq/g): BONDING Scavenger for acids and sulfonyl MECHANISM chlorides, isocyanates and weak electrophiles. Useful when charge on ion being removed is weaker than the acetate counter ion. TYPES OF FUNCTIONAL GROUPS % Organic Loading: 8.40 Exchange Capacity (meq/g): ANALYTE POLARITY Scavenger for acids and sulfonyl chlorides, isocyanates and weak electrophiles. Useful when charge on SORBENT ion being removed is weaker than the TYPE hydroxide counter ion. 37 ELUTION SOLVENT: POLYIMINE ACIDIC ELUTION Part Number Sorbent Amount/ SOLVENT Unit per Tube Volume Pack CUPAX mg/3mL 50 CUPAX mg/3mL 50 CUPAX1M6 1g/6mL 30 BASIC ELUTION SOLVENT TYPICAL APPLICATIONS: ANY CHARGED COMPOUNDS % Organic Loading: 13.5 Exchange Capacity (meq/g): 0.88 Scavenger for acids and sulfonyl chlorides, isocyanates and other electrophiles., Inc Bartram Rd Bristol, PA USA

38 CLEAN-UP ANION EXTRACTION COLUMNS QUATERNARY AMINE WITH CHLORIDE COUNTER ION Tube Volume Pack CUQAX1L1 50mg/1mL 100 CUQAX mg/1mL 100 CUQAX mg/3mL 50 CUQAX mg/3mL 50 CUQAX mg/6mL 50 CUQAX1M6 1g/6mL 30 CUQAX12Z 200mg/10mL 50 CUQAX15Z 500mg/10mL 50 CUQAX12M15 2g/15mL 20 % Organic Loading: 8.40 Exchange Capacity (meq/g): Scavenger for acids and sulfonyl chlorides, isocyanates and weak electrophiles. 38 QUATERNARY AMINE WITH ACETATE COUNTER ION Tube Volume Pack CAQAX mg/1mL 100 CAQAX mg/3mL 50 CAQAX1M6 1g/6mL 30 CAQAX15M25 5g/25mL 20 QUATERNARY AMINE WITH HYDROXIDE COUNTER ION Tube Volume Pack CHQAX1L1 50mg/1mL 100 CHQAX mg/1mL 100 CHQAX mg/3mL 50 CHQAX mg/3mL 50 CHQAX mg/6mL 50 CHQAX1M6 1g/6mL 30 CHQAX12Z 200mg/10mL 50 CHQAX12M15 2g/15mL 20 CHQAX15M25 5g/25mL 20 % Organic Loading: 8.40 Exchange Capacity (meq/g): Scavenger for acids and sulfonyl chlorides, isocyanates and weak electrophiles. Useful when charge on ion being removed is weaker than the acetate counter ion. % Organic Loading: 8.40 Exchange Capacity (meq/g): Scavenger for acids and sulfonyl chlorides, isocyanates and weak electrophiles. Useful when charge on ion being removed is weaker than the hydroxide counter ion. POLYIMINE Tube Volume Pack CUPAX mg/3mL 50 CUPAX mg/3mL 50 CUPAX1M6 1g/6mL 30 % Organic Loading: 13.5 Exchange Capacity (meq/g): 0.88 Scavenger for acids and sulfonyl chlorides, isocyanates and other electrophiles., Inc Bartram Rd Bristol, PA USA

39 CLEAN-UP CATION EXTRACTION COLUMNS CARBOXYLIC ACID Tube Volume Pack CUCCX1L1 50mg/1mL 100 CUCCX mg/1mL 100 CUCCX mg/3mL 50 CUCCX mg/3mL 50 CUCCX mg/6mL 50 CUCCX1M6 1g/6mL 30 CUCCX12Z 200mg/10mL 50 CUCCX12M15 2g/15mL 20 CUCCX15M25 5g/25mL 20 % Organic Loading: 9.10 Exchange Capacity (meq/g): Scavenger for strong anions (Quaternary amines or metals) PROPYLSULFONIC ACID 39 Tube Volume Pack CUPCX mg/1mL 100 CUPCX mg/3mL 50 CUPCX mg/3mL 50 CUPCX mg/6mL 50 CUPCX12Z 200mg/10mL 50 CUPCX15Z 500mg/10mL 50 % Organic Loading: 7.10 Exchange Capacity (meq/g): Scavenger for amines, alcohols and other nucleophiles. TRIACETIC ACID Tube Volume Pack CUTAX mg/1mL 100 CUTAX mg/3mL 50 CUTAX mg/3mL 50 CUTAX mg/6mL 50 CUTAX1M6 1g/6mL 30 CUTAX110M75 10g/75mL 10 % Organic Loading: 7.61 Exchange Capacity: Anion: 0.17 Cation: 0.06 Chelator for metal ions. i.e. tin palladium copper ruthinium chromium nickel, Inc Bartram Rd Bristol, PA USA

40 CLEAN-UP CATION EXTRACTION COLUMNS BENZENESULFONIC ACID Tube Volume Pack CUBCX1L1 50mg/1mL 100 CUBCX mg/1mL 100 CUBCX mg/3mL 50 CUBCX mg/3mL 50 CUBCX mg/6mL 50 CUBCX1M6 1g/6mL 30 CUBCX11Z 100mg/10mL 50 CUBCX12Z 200mg/10mL 50 CUBCX15Z 500mg/10mL 50 CUBCX12M15 2g/15mL 20 CUBCX110M75 10g/75mL 10 % Organic Loading: Exchange Capacity (meq/g): Scavenger for amines, alcohols and other nucleophiles. 40 BENZENESULFONIC ACID - HIGH LOAD Tube Volume Pack CUBCX1HL1L1 50mg/1mL 100 CUBCX1HL11 100mg/1mL 100 CUBCX1HL23 200mg/3mL 50 CUBCX1HL53 500mg/3mL 50 CUBCX1HL56 500mg/6mL 50 CUBCX1HLM6 1g/6mL 30 CUBCX1HL1Z 100mg/10mL 50 CUBCX1HL2Z 200mg/10mL 50 CUBCX1HL5Z 500mg/10mL 50 CUBCX1HL2M15 2g/15mL 20 CUBCX1HL5M25 5g/25mL 20 CUBCX1HL10M75 10g/75mL 10 % Organic Loading: Exchange Capacity (meq/g): Scavenger for amines, alcohols and other nucleophiles., Inc Bartram Rd Bristol, PA USA

41 CT This sorbent is composed of a silica backbone with two types of functional chains attached - an ion exchanger or polar chain and a hydrophobic carbon chain. Our copolymeric phases are produced in a way to allow for equal parts of each functional group to attach to the silica backbone. This copolymerization technique yields reproducible bonded phases and unique copolymeric chemistries which allow the controlled use of mixed mode separation mechanisms.this type of dual chemistry is beneficial especially when one is looking for both a neutral & charged compound. This is common when a neutral parent drug metabolizes & becomes a charged compound. CLEAN-UP COPOLYMERIC EXTRACTION COLUMNS Example of a Copolymeric Phase Si O OH Si O Si (CH 2 NH + 3 O OH Si Si O O OH Si C n H 2n+1 O OH Silica Backbone Hydrophobic Chain Ion Exchanger 41 Analytes Washes Elutions Cations/Anions Alkanes Alkenes Aromatics 1) Aqueous to disrupt hydrophilic interactions. 2) Methanol to disrupt residual hydrophobic and hydrophilic interferences. 1) Organic, possibly with some aqueous to elute hydrophobically bound analytes. 2) Aqueous buffer with a ph that would neutralize ionically bound analytes or an aqueous with high ionic strength or a solvent with a counter ion that would bind to sorbent., Inc Bartram Rd Bristol, PA USA

42 CLEAN-UP COPOLYMERIC EXTRACTION COLUMNS COPOLYMERIC SORBENTS & STRURES Sorbent Structure* pka Benzenesulfonic Acid (BCX2) -Si-(CH 2 ) 2 SO 3 H always charged Strong Cation Exchange Column Propylsulfonic Acid (PCX2) -Si-(CH 2 SO 3 H < 1 Strong Cation Exchange Column Carboxylic Acid (CCX2) -Si-(CH 2 ) 2 COOH 4.8 Weak Cation Exchange Column Quaternary amine (QAX2) -Si-(CH 2 N+(CH 3 always charged Strong Anion Exchange Column 42 Aminopropyl (NAX2) -Si-(CH 2 NH Weak Anion Exchange Column Cyanopropyl (CNP2) Hydrophilic Exchange Column -Si-(CH 2 CN Cyclohexyl (CYH2) -Si-(CH 2 ) Hydrophobic Exchange Column *Each copolymeric sorbent also contains a carbon chain approximately equal to a C8 chain MECHANISM OF COPOLYMERIC BONDING Using a sample composed of a theoretical neutral parent drug and its charged (acidic) metabolite, it is applied at a ph of 6 (figure 1). At this ph, many amine groups are positively charged. Since the column is also positively charged, compounds with this chemistry (cations) are repelled. Depending on the pka of the metabolite, carboxylic acid groups may be negatively charged, allowing the metabolite to bond to the positively charged sorbent. Since the column also possesses a hydrophobic chain, the neutral parent drug also bonds to the column. Example of Copolymeric Bonding O (CH 2 )n NH O C Water or a weak aqueous buffer (ph6) washes away hydrophilically bound interferences (figure 2). The column is then dried, careful to free the column of any residual aqueous phase that would interfere with elution. Sorbent Compounds, Inc Bartram Rd Bristol, PA USA

43 CLEAN-UP COPOLYMERIC EXTRACTION COLUMNS Sample Application figure 1 Sorbent NH O O C Metabolite Column Wash figure 2 Sorbent NH O O C Metabolite Sorbent O Parent Drug Sorbent + NH 3 NH + NH + Sorbent O Parent Drug Sorbent + NH 3 HO Hydrophilically Bound Compounds SH OH O Aqueous Buffer Aqueous Buffer Elution 1 figure 3 Sorbent Sorbent NH 3 + O - O C Elution 2 figure 4 Sorbent Sorbent NH The hydrophobically bound neutral parent drug is eluted with a solvent of minimal polarity, such as hexane/ethyl acetate - 80:20. Sorbent NH 3 + O Hexane / Ethyl Acetate 80:20 Parent Drug The final elution employs an acid to neutralize the charge of acidic analytes. Ionic interaction is released, and analytes are eluted in an appropriate solvent mixture. Sorbent NH 3 + O HO C Metabolite Strong Acidic Solution ph 2-3 Anion Exchange Sorbent Cation Exchange Sorbent Goal ph Goal ph WASH To promote bonding between sorbent and analyte > analyte pka or < sorbent pka To promote bonding between sorbent and analyte > analyte pka or < sorbent pka Elution To disrupt bonding between sorbent and analyte > analyte pka or < sorbent pka To promote bonding between sorbent and analyte > analyte pka or < sorbent pka Percent of Compound in Ionic State Functionality Ionization ph units away from pka 2 < pka 1 < pka at pka 1 > pka 2 > pka Acid Anionic (-) Base Cationic (+) , Inc Bartram Rd Bristol, PA USA

44 CLEAN-UP COPOLYMERIC EXTRACTION COLUMNS HYDROPHOBIC PLUS CYCLOHEXYL Tube Volume Pack CUCYH mg/6mL 50 CUCYH2M6 1g/6mL 30 % Organic Loading: N/A Exchange Capacity (meq/g): N/A Dual functionality for phenols and hydrophobic compounds. 44 HYDROPHOBIC PLUS PROPYLSULFONIC ACID Tube Volume Pack CUPCX2L1 50mg/1mL 100 CUPCX mg/1mL 100 CUPCX mg/3mL 50 CUPCX mg/6mL 50 CUPCX22Z 200mg/10mL 50 % Organic Loading: Exchange Capacity (meq/g): Dual functionality for weak bases and hydrophobic compounds. HYDROPHOBIC PLUS CARBOXYLIC ACID Tube Volume Pack CUCCX2L1 50mg/1mL 100 CUCCX mg/1mL 100 CUCCX mg/3mL 50 CUCCX mg/6mL 50 % Organic Loading: Exchange Capacity (meq/g): Dual functionality for strong base and hydrophobic compounds. HYDROPHOBIC PLUS BENZENESULFONIC ACID Tube Volume Pack CUBCX2L1 50mg/1mL 100 CUBCX mg/1mL 100 CUBCX mg/3mL 50 CUBCX mg/3mL 50 CUBCX mg/6mL 50 CUBCX2M6 1g/6mL 30 CUBCX21Z 100mg/10mL 50 CUBCX22Z 200mg/10mL 50 CUBCX25Z 500mg/10mL 50 % Organic Loading: Exchange Capacity (meq/g): Dual functionality for strong base and hydrophobic compounds. OCTADECYL PLUS BENZENESULFONIC ACID Tube Volume Pack CUBCX35Z 500mg/10mL 50 CUBCX32M15 2g/15mL 20 % Organic Loading: Exchange Capacity (meq/g): Dual functionality for strong base and hydrophobic compounds., Inc Bartram Rd Bristol, PA USA

45 CLEAN-UP COPOLYMERIC EXTRACTION COLUMNS HYDROPHOBIC PLUS QUATERNARY AMINE Tube Volume Pack CUQAX2L1 50mg/1mL 100 CUQAX mg/1mL 100 CUQAX mg/3mL 50 CUQAX mg/3mL 50 CUQAX mg/6mL 50 CUQAX2M6 1g/6mL 30 CUQAX21Z 100mg/10mL 50 CUQAX22Z 200mg/10mL 50 CUQAX25Z 500mg/10mL 50 % Organic Loading: Exchange Capacity (meq/g): Dual functionality for weak acids and hydrophobic compounds. HYDROPHOBIC PLUS AMINOPROPYL 45 Tube Volume Pack CUNAX2L1 50mg/1mL 100 CUNAX mg/1mL 100 CUNAX mg/3mL 50 CUNAX mg/3mL 50 CUNAX mg/6mL 50 CUNAX2M6 1g/6mL 30 CUNAX21Z 100mg/10mL 50 CUNAX22Z 200mg/10mL 50 CUNAX25Z 500mg/10mL 50 CUNAX22M15 2g/15mL 20 CUNAX25M25 5g/25mL 20 CUNAX210M75 10g/75mL 10 % Organic Loading: 12.3 Exchange Capacity (meq/g): Dual functionality for strong acids and hydrophobic compounds., Inc Bartram Rd Bristol, PA USA

46 CLEAN-UP COVALENT EXTRACTION COLUMNS COVALENT Covalent sorbent has aldehyde functional groups that are bound to the silica backbone by a hydrocarbon chain. The aldehyde group will react selectively with compounds containing a primary amine. A formal bond is created between the stationary support and the primary amine containing material. MECHANISM OF COVALENT BONDING The primary amine in the sample performs a nucleophilic attack on the aldehyde functional group attached to the support. This results in a Schiff base, with the amine immobilized on the stationary support. This chemistry can be utilized to bind proteins, such as antibodies, to the support, allowing highly specific extractions. 46 THIOPROPYL Tube Volume Pack CUTHX1L1 50mg/1mL 100 CUTHX mg/1mL 100 CUTHX mg/3mL 50 CUTHX mg/3mL 50 CUTHX mg/6mL 50 CUTHX1M6 1g/6mL 30 CUTHX11Z 100mg/10mL 50 CUTHX12Z 200mg/10mL 50 CUTHX15Z 500mg/10mL 50 CUTHX12M15 2g/15mL 20 CUTHX15M25 5g/25mL 20 CUTHX110M75 10g/75mL 10 % Organic Loading: 6.50 Scavenger for alkylating agents, alcohols and amines., Inc Bartram Rd Bristol, PA USA

47 STYRE SCREEN POLYMERIC RESIN EXTRACTION COLUMNS 47 w w w. u n i t e d c h e m. c o m, Inc Bartram Rd Bristol, PA USA

48 STYRE SCREEN POLYMERIC RESIN EXTRACTION COLUMNS Styre Screen extraction columns contain an ultra clean, highly cross-linked styrene and divinylbenzene copolymer sorbent that is functionalized with both a reverse phase, hydrophobic component and a strong cation exchanger. High and reproducible recoveries for acidic, neutral and basic compounds are achievable with a single column. The Styre Screen particles have an average particle size of 30 microns and a very high analyte capacity making them ideal for standard solid phase extraction applications. The increased analyte capacity means that less sorbent bed mass is needed which results in faster flow rates and less solvent use. Higher throughput and less solvent waste disposal translate into significant savings in both time and money. In addition, no conditioning steps are required for most drugs of abuse applications. DBX - BENZENESULFONIC ACID + C18 48 Tube Volume Pack SSDBX031 30mg/1mL 100 SSDBX033 30mg/3mL 50 SSDBX056 50mg/6mL 50 DVB - POLYSTYRENE DIVINYLBENZENE Tube Volume Pack SSDVB031 30mg/1mL 100 SSDVB033 30mg/3mL 50 SSDVB056 50mg/6mL 50 BCX - ION EXCHANGE Tube Volume Pack SSBCX031 30mg/1mL 100 SSBCX033 30mg/3mL 50 SSBCX056 50mg/6mL 50 C18 - REVERSE PHASE Tube Volume Pack SSC mg/1mL 100 SSC mg/3mL 50 SSC mg/6mL 50 CCX - CARBOXYLIC ACID Tube Volume Pack SSCCX031 30mg/1mL 100 SSCCX033 30mg/3mL 50 SSCCX056 50mg/6mL 50 QAX - QUATERNARY AMINE Tube Volume Pack SSQAX031 30mg/1mL 100 SSQAX033 30mg/3mL 50 SSQAX056 50mg/6mL 50 Dual functionality for weak acids and hydrophobic compounds. N/A Scavenger for amines, alcohols and other nucleophiles. Removes hydrophobic impurities, de-salting and purification of hydrophobic compounds. Scavenger for strong anions. (Quaternary amines or metals) Removes large or more hydrophobic compounds., Inc Bartram Rd Bristol, PA USA

49 SELECTRASORB BULK SORBENTS PACKING MATERIAL HYDROPHOBIC Description Part Number Part Number Sizes Endcapped Unendcapped C8, OCTYL CEC0800X CUC0800X 10g CEC0800C CUC0800C 100g CEC0800K CUC0800K 1kg C18, OCTADECYL Tri-Functional CEC1800X CUC1800X 10g CEC1800C CUC1800C 100g CEC1800K CUC1800K 1kg COPOLYMERIC BONDED PHASES FOR DRUG ABUSE TESTING Description Part Number Sizes CSDAU CSTHC COPOLYMERIC Description Part Number Part Number Sizes Endcapped Unendcapped BENZENESULFONIC ACID + C8 CARBOXYLIC ACID + C8 QUATERNARY ACID + C8 AMINOPROPYL + C8 CSDAU00X CSDAU00C CSDAU00K CSTHC00X CSTHC00C CSTHC00K 10g 100g 1kg 10g 100g 1kg CUBCX20X CUBCX20C CUBCX20K CUCCX20X CUCCX20C CUCCX20K CUQAX20X CUQAX20C CUQAX20K CUNAX20X CUNAX20C CUNAX20K 10g 100g 1kg 10g 100g 1kg 10g 100g 1kg 10g 100g 1kg HYDROPHILIC Description Part Number Part Number Sizes Endcapped Unendcapped CN,CYANOPROPYL CECNP00X CUCNP00X 10g CECNP00C CUCNP00C 100g CECNP00K CUCNP00K 1kg UNBONDED SILICA μm CUSIL00X 10g CUSIL00C 100g CUSIL00K 1kg PHARMA-SIL μm PHSIL00X 10g PHSIL00C 100g PHSIL00K 1kg High Surface Activity Silica μm HSSIL00X 10g HSSIL00C 100g HSSIL00K 1kg DIOL FLORISIL ALUMINA, ACIDIC ALUMINA, BASIC ALUMINA, NEUTRAL CUDOL00X CUDOL00C CUDOL00K CUFLS00X CUFLS00C CUFLS00K CUALA00X CUALA00C CUALA00K CUALB00X CUALB00C CUALB00K CUALN00X CUALN00C CUALN00K 10g 100g 1kg 10g 100g 1kg 10g 100g 1kg 10g 100g 1kg 10g 100g 1kg 49, Inc Bartram Rd Bristol, PA USA

50 SELECTRASORB BULK SORBENTS ION EXCHANGE ( ANION ) Description Part Number Sizes ION EXCHANGE ( CATION ) Description Part Number Sizes N-2 AMINOETHYL CUPSA00X CUPSA00C CUPSA00K 10g 100g 1kg CARBOXYLIC ACID CUCCX00X CUCCX00C CUCCX00K 10g 100g 1kg AMINOPROPYL CUNAX00X CUNAX00C CUNAX00K 10g 100g 1kg PROPYLSULFONIC ACID CUPCX00X CUPCX00C CUPCX00K 10g 100g 1kg DIETHYLAMINO CUDAX00X CUDAX00C CUDAX00K 10g 100g 1kg BENZENESULFONIC ACID CUBCX00X CUBCX00C CUBCX00K 10g 100g 1kg 50 QUATERNARY AMINE with CHLORIDE QUATERNARY AMINE with ACETATE COUNTER ION CUQAX00X CUQAX00C CUQAX00K COUNTER ION CAQAX00X CAQAX00C CAQAX00K 10g 100g 1kg 10g 100g 1kg BENZENESULFONIC ACID ( HIGH LOAD ) CUBCX1HL00X CUBCX1HL00C CUBCX1HL00K TRI-ACETIC ACID CUTAX00X CUTAX00C CUTAX00K STYRE SCREEN 10g 100g 1kg 10g 100g 1kg QUATERNARY AMINE with HYDROXIDE COUNTER ION CHQAX00X CHQAX00C CHQAX00K 10g 100g 1kg Description Part Number Sizes DBX-BENZENESULFONIC ACID + C8 SSDBX00X 10g SSDBX00C 100g POLYIMINE CUPAX00X CUPAX00C CUPAX00K 10g 100g 1kg DVB POLSTYRENE DIVINYLBENZENE SSDVB00X SSDVB00C 10g 100g C18 REVEERSE PHASE OCTADECYL SSC1800X SSC1800C 10g 100g BCX-BENZENESULFONIC ACID SSBCX00X SSBCX00C 10g 100g QAX QUATERNARY AMINE SSQAX00X SSQAX00C 10g 100g, Inc Bartram Rd Bristol, PA USA

51 RESERVOIRS EMPTY POLYPROPYLENE RESERVOIRS Part Number Description Empty Reservoirs Standard Configuration Units RFV0001P 1 ml capacity 50 RFV0004P 4 ml capacity 50 RFV0008P 8 ml capacity 50 RFV0010P 10 ml capacity 50 RFV0015P 15 ml capacity 50 RFV0025P 25 ml capacity 50 RFV0075P 75 ml capacity 20 RFV0150P 150 ml capacity 8 FLANGELESS RESERVOIRS Part Number Description Units RFT00R3P Empty Flangeless Tube, 50 4 ml capacity 51 RFT1FR3P Empty Flangeless Tube, 50 1 Frit (Filter Tube) 4 ml capacity GLASS RESERVOIRS Part Tube Frit Tube Number Volume Description Description Units RFV0008G 6 ml No Frits Empty Glass Tube 30 RFV01F8G 6 ml 1 PTFE Frit Inserted Glass Tube 30 FR10081T 6 ml PTFE Frit Only None 60 CARTRIDGES Part Number Description Empty Reservoirs Standard Configuration Units RFTO2FLC Large cartridges w/cap, 1 Frit Loose, 1 Frit Inserted 50 RFTO2FMC Medium cartridges w/cap, 1 Frit Loose, 1 Frit Inserted 50 RFTO2FSC Small cartridges w/cap, 1 Frit Loose, 1 Frit Inserted 50, Inc Bartram Rd Bristol, PA USA

52 RESERVOIRS 52 FRITTED POLYPROPYLENE RESERVOIRS Part Number Part Number Fritted Reservoirs Fritted Reservoirs Description 1 Frit, 10 Micron Porosity 2 Frits, 10 Micron Porosity Standard Configuration Units RFV01F1P RFV02F1P 1 ml capacity 50 RFV01F4P RFV02F4P 4 ml capacity 50 RFV01F8P RFV02F8P 8 ml capacity 50 RFV1F10P RFV2F10P 10 ml capacity 50 RFV1F15P RFV2F15P 15 ml capacity 50 RFV1F25P RFV2F25P 25 ml capacity 50 RFV1F75P RFV2F75P 75 ml capacity 20 RFV1F150P RFV2F150P 150 ml capacity 10 Part Number Part Number Fritted Reservoirs Fritted Reservoirs Description 1 Frit, 20 Micron Porosity 2 Frits, 20 Micron Porosity Standard Configuration Units RFT01F1P RFT02F1P 1 ml capacity 50 RFT01F4P RFT02F4P 4 ml capacity 50 RFT01F8P RFT02F8P 8 ml capacity 50 RFT01F10P RFT2F10P 10 ml capacity 50 RFT01F15P RFT2F15P 15 ml capacity 50 RFT01F25P RFT2F25P 25 ml capacity 50 RFT01F75P RFT2F75P 75 ml capacity 20 RFT1F150P RFT2F150P 150 ml capacity 10, Inc Bartram Rd Bristol, PA USA

53 FRITS POROUS POLYETHYLENE FRITS Part Number Description 10 Micron Porosity (1/16 thickness) Standard Configuration Units FR10011P 1 ml capacity 100 FR10041P 4 ml capacity 100 FR10081P 8 ml capacity 100 FR10101P 10 ml capacity 100 FR10151P 15 ml capacity 100 FR10251P 25 ml capacity 100 FR10751P 75 ml capacity Part Number Description 20 Micron Porosity (1/16 thickness) Standard Configuration Units FR20011P 1 ml capacity 100 FR20041P 4 ml capacity 100 FR20081P 8 ml capacity 100 FR20101P 10 ml capacity 100 FR20151P 15 ml capacity 100 FR20251P 25 ml capacity 100 FR20751P 75 ml capacity 100 Part Number Description 20 Micron Porosity (1/8 thickness) Standard Configuration Units FT20011P 1 ml capacity 100 FT20041P 4 ml capacity 100 FT20081P 8 ml capacity 100 FT20101P 10 ml capacity 100 FT20151P 15 ml capacity 100 FT20251P 25 ml capacity 100 PTFE FRITS Part Number Description Units FR10081T 6 ml capacity 60 FR10041T 3 ml capacity 60, Inc Bartram Rd Bristol, PA USA

54 SOLID PHASE EXTRACTION ACCESSORIES STOPCOCK VALVES Quantity Part Number 16 VMF VMF02024 Solvent resistant, reusable luer fitted valves to be used in conjunction with a vacuum manifold. Provides individual flow control to each SPE reservoir. U ADAPTERS Quantity Part Number 15 AD0000AS 54 Adapter cap has a tapered fit for 1, 3 and 6mL size reservoirs with a standard luer fitting on top. These adapters are ideal when a sample volume exceeds the capacity of the SPE column or when sequential extractions are desired. FLANGE CAPS Part Number Cap Volume Quantity CR0001P 1 ml 50 CR0004P 3 ml 50 CR0008P 6 ml 50 CR0015P 15 ml 50 CR0025P 25 ml 50 Inlet caps plug the top of SPE tubes. MALE LUER CAPS Quantity Part Number 50 LUER50 Fits a standard luer, for all reservoir sizes. CLEAN-THRU TIPS Quantity Part Number 50 CLTTP050 Eliminates sample carry over from the vacuum manifold lid. Tips connect to the luer tip on the SPE reservoir and are passed through the manifold, directly into the waste or collection vessel. They provide a completely disposable system that eliminates any contact between the sample, or wash solvents and the extraction apparatus., Inc Bartram Rd Bristol, PA USA

55 CT Air supply: N2 or compressed air 75psi (must be Features: All 48 positions are individually regulated to provide even pressure to each column 4 banks of 12 positions. Individual flow indicator on each bank. Pressure always provided to bank 1. Pressure supply to banks 2-4 individually controlled Capacity 1 to 48 columns of either 1mL, 3mL, 6mL, 10mL or 15mL filtered to 10 μm) Dual pressure regulators allow users to set 2 different pressures for extraction and column drying POSITIVE PRESSURE MANIFOLD Includes waste reservoir that can be emptied between each waste step if desired Single switch raises and lowers the sample racks and creates an airtight seal COMPLETE POSITIVE PRESSURE MANIFOLD SYSTEM INCLUDES: Part Number Description Unit VMFPPM13 10mL/15mL SPE tube rack, 13 x 100 collection rack, 1 pre-drilled waste container and collection rack of choice 55 VMFPPM16 10mL/15mL SPE tube rack, 16 x 100 collection rack, 1 pre-drilled waste container and collection rack of choice POSITIVE PRESSURE MANIFOLD ACCESSORIES Part Number Description Unit VMFPPMRK10 10 ml & 15 ml SPE rack. 1 VMFPPMRKA1 Adapter extraction plates for 1 ml columns. 1 VMFPPMRKA3 Adapter extraction plates for 3 ml columns. 1 VMFPPMRKA6 Adapter extraction plates for 6 ml columns. 1 VMFPPMIK Installation kit (contains 25 ft ¼ O.D. tubing, in line air filter, ¼ compression fittings, one (1) set of gaskets.) VMFPPMCRKG13 Collection rack for 13 x 10 mm test tubes 1 VMFPPMCRKG16 Collection rack for 16 x 10 mm test tubes 1 VMFPPMWRK Waste rack 1 VMFPPMWBN Waste container (pre-drilled for optional tubing attachment.) 1 VMFPPRAF Replacement in line air filter. 1 VMFPPMGSKBL Brown PPM Gasket 1 VMFPPMGSKOR Orange PPM Gasket 1, Inc Bartram Rd Bristol, PA USA VMFPPMLPLT Lift Plate 1

56 Collection rack for 13 x 100mm test tubes Part#: VMFPPMCRKG13 Collection rack for 16 x 100mm test tubes Part#: VMFPPMCRKG16 Pre-drilled waste container Part#: VMFPPMWBND PPM Waste tray draining kit; 10 ft tube and stopcock Part#: VMFPPMWTDK 56 Adaptor extraction plates for 1mL columns Part#: VMFPPMRKA1 Adaptor extraction plates for 3mL columns Part#: VMFPPMRKA3 Adaptor extraction plates for 6mL columns Part#: VMFPPMRKA6 10mL /15mL extraction plates Part#: VMFPPMRK10 Brown PPM Gasket Part#: VMFPPMGSKBL Orange PPM Gasket Part#: VMFPPMGSKOR Restrictor Plate Part#: VMFPPMFRPLT CT, Inc Bartram Rd Bristol, PA USA Installation kit (contains 25 1/4 O.D. tubing, in line air filter, 2-1/4 compression fittings, one (1) set of gaskets Part#: VMFPPMIK In-line air filter Part#: VMFPPMRAF

57 UNIVERSAL VACUUM MANIFOLD UNIVERSAL VACUUM MANIFOLD COMPLETE SYSTEM Part Number: VMFUVWP A manifold system that accommodates a 24, 48 and 96 well plate configuration. System includes collection plate, extraction plate, manifold and vacuum gauge waste base. Extraction and collection plates for 24 well and 48 well configurations are included. Erlenmeyer flask and tubing are included WELL PLATE MANIFOLD SYSTEM Part Number: VMF24WP System includes 24 well collection plate, extraction plate, manifold and vacuum gauge waste base. Extraction plate can accommodate (24) 6 ml SPE columns. SPE columns can be packed in 500 mg,1 and 2 gram formats. Collection plate will accommodate 16x100mm and 16x125mm test tubes. 48 WELL PLATE MANIFOLD SYSTEM Part Number: VMF48WP System includes 48 well collection plate, extraction plate, manifold and vacuum gauge waste base. Extraction plate can accommodate (48) 4.5 ml SPE columns in a staggered array. Elute samples directly into 12 x 75 mm test tube or 2 ml analytical vials. 96 DEEP WELL PLATE MANIFOLD SYSTEM Part Number: VMF96WP System includes manifold and vacuum gauge waste base. Well plates are separate and not included with system. Accommodates most standard 96 well plates. Collect directly into microplate., Inc Bartram Rd Bristol, PA USA

58 UNIVERSAL MANIFOLD ACCESSORIES Description: 24 Well Extraction Plate Part Number: VMF24EP Unit: 1 Description: 24 Well Collection Plate Part Number: VMF24CP Unit: 1 Description: 48 Well Extraction Plate Part Number: VMF48EP Unit: 1 Description: 48 Well Collection Plate Part Number: VMF48CP Unit: 1 58 Description: Manifold Top Part Number: VMFUVMT Unit: 1 Description: Neoprene Gasket Part Number: VMFUVNG Unit: 1 Description: Manifold Base Part Number: VMFUVMB Unit: 1 Description: EPDM Gasket Part Number: VMFUVEG Unit: 1 Description: Spacer 1/2 Part Number: VMFUV05SP Unit: 1 Description: Spacer 1 Part Number: VMFUV10SP Unit: 1 Description: Collection Plate Riser Part Number: VMFUVR1 Unit: 1 Description: Spigot Part Number: VMFUVST Unit: 1 Description: Vacuum Base & Waste Gauge Part Number: VMFUVVGWB Unit: 1 Description: Vacuum Pump Part Number: ECROCKER400 Unit: 1, Inc Bartram Rd Bristol, PA USA

59 VACUUM MANIFOLD Our vacuum Manifold System consists of a rigid Corian lid which resists warping with extended use. The lid is outfitted with bulkhead luer fittings to allow for sample to elute directly into disposable test tubes. The lid is available with 16 or 24 positions, consisting of two parallel rows and corner leg supports to prevent damage to the luers and tips when the lid is not being used. The vacuum chamber is a glass block designed to allow for visible inspection of the extraction process and ease when cleaning. The block is equipped with a vacuum control valve for optimizing sample flow rate during the extraction process. A vacuum gauge and bleed valve allows for easy monitoring of the vacuum process. Polypropylene racks are provided according to the number of positions on the manifold lid and are highly resistant to chemical degradation and abuse. The test tube racks provided allow the use of mm or 16 mm disposable test tubes. These manifold systems are durable and chemically resistant units designed to provide years of trouble-free extractions. A safety tray comes as part of the complete manifold system, so as to prevent the glass block from cracking or chipping. COMPLETE VACUUM MANIFOLD SYSTEM - 16 POSITION SYSTEM INCLUDES: A glass block, Corian lid, Cover gasket, Vacuum gauge, 16 PTFE tips, Adjustable collection rack, Bulkhead luer fittings, 16 plugs and a glass block safety tray Unit 1 Part Number VMF016GL 59 COMPLETE VACUUM MANIFOLD SYSTEM - 24 POSITION SYSTEM INCLUDES: A glass block, Corian lid, Cover gasket, Vacuum gauge, 24 PTFE tips, Adjustable collection rack, Bulkhead luer fittings, 24 plugs and a glass block safety tray Unit 1 Part Number VMF024GL VACUUM MANIFOLD ACCESSORIES Description Units Part Number 16 Position System Part Number 24 Position System Glass Block 1 VMF06123 VMF04123 Manifold Lid 1 VMF06120 VMF04120 Gasket 2 VMF04121 VMF04121 Collection Rack 1 VMF06125 VMF04125 Description Units Part Number for 16 or 24 Position Vacuum Gauge & Valve Assembly 1 VMF02122 Retaining Clips for Collection Rack 12 VMF02129 Bulkhead Luer Fittings 12 VMF21BFN Manifold Lid Legs 4 VMF Luer Plugs 12 VMF21PLN Luer Caps 50 LUER50 Clean-Thru Tips 50 CLTTP050 PTFE Luer Tips 12 VMF020TT Manifold Safety Tray 1 VMF02072 Stopcocks 16 VMF VMF02024 Vacuum Pump 1 ECROCKER400-1/8 hp, 115 volts, 4.2 amps, 60 Hz NOTE: A Complete Vacuum Manifold System includes a glass block,, Corian Inc manifold Bartram lid, Rd a cover Bristol, gasket, PA a vacuum USA gauge and valve assembly, PTFE tips (16 or 24), an adjustable collection rack, bulkhead luer fittings, plugs (16 or 24), and a glass block safety tray.

60 SELECT ph BUFFER POUCHES PHOSPHATE AND ACETATE BUFFER POUCHES To help simplify the process of sample preparation, has developed a line of ready-to-use phosphate and acetate buffer pouches. The buffer pouches are a convenient way of accurately preparing the necessary reagents, at the proper ph and concentration, for solid phase extraction methods. These premeasured pouches eliminate time and more importantly, any potential error in the buffer preparation, insuring the highest efficiency in the extraction method. As with all products, these buffer pouches are prepared with the same high quality standards used in the manufacture of the entire line of SPE products. SELECT ph BUFFER POUCHES 100mM ACETATE ph Part Number: SPHACE (Pack of 5) SPHACE (Pack of 10) Contents: 5.86 g Sodium Acetate Trihydrate Instructions: Add 300 ml of deionized water to a 500 ml volumetric flask. Mix in the contents of the buffer pouch and shake/stir well. Add 3.24 ml of glacial acetic acid to the volumetric flask and dilute to the mark with deionized water. Ensure ph is 4.5+/-0.5. Yield: 500 ml of solution SELECT ph BUFFER POUCHES 100mM ACETATE ph 5.00 Part Number: SPHACE (Pack of 5) SPHACE (Pack of 10) Contents: 4.29 g Sodium Acetate Trihydrate Instructions: Add 300 ml of deionized water to a 500 ml volumetric flask. Mix in the contents of the buffer pouch and shake/stir well. Add 1.04 ml of glacial acetic acid to the volumetric flask and dilute to the mark with deionized water. Ensure ph is 5.0+/-0.5. Yield: 500 ml of solution SELECT ph BUFFER POUCHES 1M ACETATE ph 5.0 Part Number: SPHACE (Pack of 5) SPHACE (Pack of 10) Contents: 42.9 g Sodium Acetate Trihydrate Instructions: Add 300 ml of deionized water to a 500 ml volumetric flask. Mix in the contents of the buffer pouch and shake/stir well. Add 10.4 ml of glacial acetic acid to the volumetric flask and dilute to the mark with deionized water. Ensure ph is 5.0+/-0.5. Yield: 500 ml of solution SELECT ph BUFFER POUCHES 100mM PHOSPHATE ph 6.0 Part Number: SPHPHO (Pack of 5) SPHPHO (Pack of 10) Contents: 1.70 g Disodium Hydrogen Phosphate and g Sodium Dihydrogen Phosphate Hydrate Instructions: Add 600 ml of deionized water to a 1000 ml volumetric flask. Add in the contents of the buffer pouch and mix/stir. Dilute to the mark with deionized water. Ensure the ph is 6.0+/-0.5. Yield: 1000 ml of solution SELECT ph BUFFER POUCHES 100mM PHOSPHATE ph 7.0 Part Number: SPHPHO (Pack of 5) SPHPHO (Pack of 10) Contents: 7.82 g Disodium Hydrogen Phosphate and 6.22 g Sodium Dihydrogen Phosphate Hydrate Instructions: Add 600 ml of deionized water to a 1000 ml volumetric flask. Add in the contents of the buffer pouch and mix/stir. Dilute to the mark with deionized water. Ensure the ph is 7.0+/-0.5. Yield: 1000 ml of solution, Inc Bartram Rd Bristol, PA USA

61 SELECTRA-SIL DERIVATIZING REAGENTS 61 BENEFITS OF DERIVATIZATION Improved chromatographic resolution - increased volatility - reduced intermolecular hydrogen bonding - separation of structurally similar compounds. Improved mass spectral characteristics - higher mass fragments - greater S/N (signal to noise ratio) - more unique massesincreased abundance/sensitivity of molecular ions. Improved thermal stability of some compounds - reduced thermal degradation - higher temperatures to speed analysis. Increased instrument and lab productivity - fewer reinjections or repeats due to peak tailing - high confidence in analyte identification and quantitation - easy to perform - inert by-products of derivatization will not degrade capillary column performance. PROD RECOMMENDATIONS Derivatizing reagents are sold bottled in airtight septum vials blanketed with nitrogen to maintain their activity. Contact with moisture can greatly reduce reactivity. Protect from air and moisture. Keep bottle tightly sealed when not in use. It is critical that pipettes, glassware, vials, etc. be free of water or alcohol before derivatization., Inc Bartram Rd Bristol, PA USA

62 SELECTRA-SIL SILYLATION REAGENTS Silyl derivatives are the most widely used chemical derivatization reagents, especially for gas chromatography. Silyl derivatization requires an Active hydrogen as seen in acids, alcohols, thiols, amines, amides, enolizable ketones and aldehydes to be replaced by a trimethysilyl group or tertiary butyl dimethylsilyl. BSTFA N,O-bis(Trimethylsilyl)trifluoroacetamide Part Number Quantity Units per pack SBSTFA gm vial 10 SBSTFA gm vial 1 SBSTFA gm vial 1 SBSTFA gm vial 1 Trimethylsilyl donor strength equal to BSA. Reacts with the same classes of compounds as BSA producing the same derivatives. Increased volatility of the reaction by-products over the non-fluorinated derivatives of BSA. 62 BSTFA W/1% TMCS N,O-bis(Trimethylsilyl)trifluoroacetamide w/1% Trimethylchlorosilane Part Number Quantity Units per pack SBSTFA gm vial 10 SBSTFA gm vial 1 SBSTFA gm vial 1 SBSTFA gm vial 1 Trimethylsilyl donor strength equal to BSA. Reacts with the same classes of compounds as BSA producing the same derivatives. TMCS (Trimethylchlorosilane) added to derivatize amides, many secondary amines and hindered hydroxyls that are not reactive to BSTFA alone. Increased volatility of the reaction by-products over the non-fluorinated derivatives of BSA. BSTFA W/10% TMCS N,O-bis(Trimethylsilyl)trifluoroacetamide w/10% Trimethylchlorosilane Part Number Quantity Units per pack SBSTFA gm vial 10 SBSTFA gm vial 1 SBSTFA gm vial 1 SBSTFA gm vial 1 Trimethylsilyl donor strength equal to BSA. Reacts with the same classes of compounds as BSA producing the same derivatives. TMCS (Trimethylchlorosilane) added to derivatize amides, many secondary amines and hindered hydroxyls that are not reactive to BSTFA alone. Increased volatility of the reaction by-products over the non-fluorinated derivatives of BSA. MSTFA N-Methyl-N-trimethylsilyltrifluoroacetamide Part Number Quantity Units per pack SMSTFA gm vial 10 SMSTFA gm vial 1 SMSTFA gm vial 1 SMSTFA gm vial 1 A trimethylsilyl adduct with donor strength equal to BSA and BSTFA. Most volatile of the TMS derivatives. Often elutes at the solvent front of the GC. MSTFA W/1% TMCS N-Methyl-N-trimethylsilyltrifluoroacetamide w/1% trimethylchlorosilane Part Number Quantity Units per pack SMSTFA gm vial 10 SMSTFA gm vial 1 SMSTFA gm vial 1 SMSTFA gm vial 1 A trimethylsilyl adduct with donor strength equal to BSA and BSTFA. Most volatile of the TMS derivatives. Often elutes at the solvent front of the GC. Addition of TMCS aids in the derivatization of amides, secondary amines and hindered Hydroxy groups., Inc Bartram Rd Bristol, PA USA

63 SELECTRA-SIL SILYLATION REAGENTS MTBSTFA N-Methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide Part Number Quantity Units per pack SMTBSTFA gm vial 10 SMTBSTFA gm vial 1 SMTBSTFA gm vial 1 SMTBSTFA gm vial 1 Derivatizes hydroxyl, carboxyl, thiol and primary and secondary amines. Derivatives are more stable than TMS derivatives to hydrolysis. Reaction by-products are neutral and volatile. MTBSTFA W/1% TBDMCS N-Methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide w/ 1% tert-butyldimethylchlorosilane Part Number Quantity Units per pack SMTBSTFA gm vial 10 SMTBSTFA gm vial 1 SMTBSTFA gm vial 1 SMTBSTFA gm vial 1 Derivatizes hydroxyl, carboxyl, thiol and primary and secondary amines. Addition of TBDMCS (tertiary butyl-dimethylchlorosilane) increases the silylation ability of this reagent to derivatize hindered alcohols and amines. Derivatives are more stable than TMS derivatives to hydrolysis. Reaction by-products are neutral and volatile. MTBSTFA W/10% TBDMCS N-Methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide 63 Part Number Quantity Units per pack SMTBSTFA gm vial 10 SMTBSTFA gm vial 1 SMTBSTFA gm vial 1 SMTBSTFA gm vial 1 TMCS Trimethylchlorosilane Derivatizes hydroxyl, carboxyl, thiol and primary and secondary amines. Addition of TBDMCS (tertiary butyl-dimethylchlorosilane) increases the silylation ability of this reagent to derivatize hindered alcohols and amines. Derivatives are more stable than TMS derivatives to hydrolysis. Reaction by-products are neutral and volatile. Part Number Quantity Units per pack STMCS gm vial 10 STMCS gm vial 1 STMCS gm vial 1 STMCS gm vial 1 Catalysts used to increase the reactivity of other silylation reagents. Used to form trimethysilyl esters of organic acids. SELECTRA-SIL SOLVENTS FOR DERIVATIZING REAGENTS Solvent Part Number Size Units Acetonitrile (ACN) SACN ml vial 1 Pyridine SPYR ml vial 1 Pyridine SPYR ml vial 1 Reagents can be packed into sealed glass ampules upon request., Inc Bartram Rd Bristol, PA USA

64 SELECTRA-SIL ALKYLATION REAGENTS Silyl derivatives are the most widely used chemical derivatization reagents, especially for gas chromatography. Silyl derivatization requires an Active hydrogen as seen in acids, alcohols, thiols, amines, amides, enolizable ketones and aldehydes to be replaced by a trimethysilyl group or tertiary butyl dimethylsilyl. TMPAH 0.2M Trimethylanilium hydroxide in Methanol Part Number Quantity Units per pack STMPAH gm vial 10 STMPAH gm vial 1 STMPAH gm vial 1 STMPAH gm vial 1 Used for the methylation of barbiturates, sedatives, xanthines,and alkaloids by GC. The derivatization of these compounds often can be done in the injector of the GC CB 4-Carbethoxyhexafluorobutyrl Chloride Part Number Quantity Units per pack S4CB gm vial 10 S4CB gm vial 1 S4CB gm vial 1 SELECTRA-SIL SPECIALIZED REAGENTS PFPOH Pentafluoropropanol Peptides + Propionic anhydride converts N-termini and Lysines to propyl amides. This results in a decrease in net charge of the peptides and increased hydrophobicity. Part Number Quantity Units per pack SPFPOH gm vial 10 SPFPOH gm vial 1 SPFPOH gm vial 1 SPFPOH gm vial 1 Used in combination with the acid anhydrides to add more fluorine atoms to the parent molecule. This reaction is especially used for carboxylic acids. The addition of fluorine atoms into the molecule greatly adds to the sensitivity of certain detectors (i. e. ECD, MS). HFIP Hexafluoro-2-propanol Part Number Quantity Units per pack SHFIP gm vial 10 SHFIP gm vial 1 SHFIP gm vial 1 SHFIP gm vial 1 Used in combination with the acid anhydrides to add more fluorine atoms to the parent molecule. This reaction is especially used for carboxylic acids. The addition of fluorine atoms into the molecule greatly adds to the sensitivity of certain detectors (i. e. ECD, MS), Inc Bartram Rd Bristol, PA USA

65 SELECTRA-SIL ACYLATION REAGENTS MBTFA N-methyl-bis-trifluoroacetamide Part Number Quantity Units per pack SMBTFA gm vial 10 SMBTFA gm vial 1 SMBTFA gm vial 1 SMBTFA gm vial 1 This reacts with primary and secondary amines, hydroxyl and thiol groups under mild, nonacidic conditions. Produces very volatile derivatives of carbohydrates. Can be used to selectively acylate amines in the presence of hydroxyl and carboxyl groups that have been protected by silylation. TFAA Trifluoroacetic acid anhydride Part Number Quantity Units per pack STFAA gm vial 10 STFAA gm vial 1 STFAA gm vial 1 STFAA gm vial 1 Reacts readily with alcohols, phenols and amines producing stable volatile derivatives for TCD, FID, ECD and other detectors. Most reactive of all the perfluoroacid anhydrides and frequently used to identify methamphetamine. PFAA Pentafluoropropionic acid anhydride 65 Part Number Quantity Units per pack SPFAA gm vial 10 SPFAA gm vial 1 SPFAA gm vial 1 SPFAA gm vial 1 HFAA Heptafluorobutyric acid anhydride Reacts readily with alcohols, phenols and amines producing stable volatile derivatives for TCD, FID. Commonly used in the determination of Benzoylecgonine and Opiates. Acidic by-products of this reaction must be removed before the derivative can be injected onto the GC. Part Number Quantity Units per pack SHFAA gm vial 10 SHFAA gm vial 1 SHFAA gm vial 1 SHFAA gm vial 1 Reacts readily with alcohols, phenols and amines producing stable volatile derivatives for TCD, FID,ECD and other detectors. Used in the determination of Amphetamines and Phencyclidine. It is suggested that amine bases (such as triethylamine) be used to react with the acidic by-products of these to drive the reaction to completion. TFAI - N-Trifluoroacetylimidazole Part Number Quantity Units per pack STFAI gm vial 10 STFAI gm vial 1 Reacts readily with alcohols, phenols and amines producing stable volatile derivatives for TCD, FID, ECD and other detectors. Offer considerable advantages over the anhydrides for the preparation of perfluoroacyl derivatives; the reactions are smooth, quantitative and produce no acid byproducts. Principal byproduct is imidazole (relatively inert). PIA Propionic Anhydride Part Number Quantity Units per pack SPIA gm vial 10 SPIA gm vial 1 SPIA gm vial 1, Inc Bartram Rd Bristol, PA USA

66 GC LINERS 66 Description Instrument / Liner Part Number Part Number Quantity 2mm Straight Splitless 2.0mm ID x 6.5mm OD x 78.5mm 2mm Straight Splitless with Deactivated Glass Wool 2.0mm ID x 6.5mm OD x 78.5mm 2mm Splitless Gooseneck 2.0mm ID x 6.5mm OD x 78.5mm GCL2MM 1 Pack Agilent GCL2MM-5 5 Pack GCL2MM Pack GCL2MMGW 1 Pack Agilent GCL2MMGW-5 5 Pack GCL2MMGW Pack GCLGN2MM 1 Pack Agilent GCLGN2MM-5 5 Pack GCLGN2MM Pack 2mm Splitless Gooseneck with Deactived Glass Wool 2.0mm ID x 6.5mm OD x 78.5mm 4mm Straight Splitless 4.0mm ID x 6.5mm OD x 78.5mm 4mm Straight Splitless with Deactivated Glass Wool 4.0mm ID x 6.5mm OD x 78.5mm 4mm Splitless Recessed Gooseneck 4.0mm ID x 6.5mm OD x 78.5mm 4mm Splitless Recessed Gooseneck with Deactived Glass Wool 4.0mm ID x 6.5mm OD x 78.5mm 4mm Splitless Gooseneck 4.0mm ID x 6.5mm OD x 78.5mm 4mm Splitless Gooseneck with Deactived Glass Wool 4.0mm ID x 6.5mm OD x 78.5mm GCLGN2MMGW 1 Pack Agilent GCLGN2MMGW-5 5 Pack GCLGN2MMGW Pack GCL4MM 1 Pack Agilent GCL4MM-5 5 Pack GCL4MM Pack GCL4MMGW 1 Pack Agilent GCL4MMGW-5 5 Pack GCL4MMGW Pack GCLRG4MM 1 Pack Agilent GCLRG4MM-5 5 Pack GCLRG4MM Pack GCLRG4MMGW 1 Pack Agilent GCLRG4MMGW-5 5 Pack GCLRG4MMGW Pack GCLGN4MM 1 Pack Agilent GCLGN4MM-5 5 Pack GCLGN4MM Pack GCLGN4MMGW 1 Pack Agilent GCLGN4MMGW-5 5 Pack GCLGN4MMGW Pack, Inc Bartram Rd Bristol, PA USA

67 SELECTRA HPLC COLUMNS HPLC COLUMN Part Number SLPHY100ID21 SLPHY50ID21 Description Phenyl HPLC Column - 2.1mm x 100mm Phenyl HPLC Column - 2.1mm x 50mm Unit 1 1 SLC18100ID21 SLC1850ID21 C18 HPLC Column - 2.1mm x 100mm C18 HPLC Column - 2.1mm x 50mm 1 1 GUARD COLUMN Part Number SLPHYGDC Description Phenyl HPLC Guard Column Unit 1 SLC18GDC C18 HPLC Guard Column Bartram Rd Bristol, PA USA

68 96 DEEP WELL PLATES IDEAL FOR ALL SPE APPLICATIONS AND HIGH THROUGHPUT SCREENING Our 96 Deep Well Plates are made from solvent resistant, low extractable polypropylene. Standard frits are polyethylene with 20μ pores. 96 DEEP WELL FILTER PLATES 68 Description Part Number Units Empty 96 deep well plate with one frits inserted WSH961FR 1 Loose 96 deep well plate square frits FRSH2096P well collection plate WSH96CP 1 96 well plate sealable lid WSH96PS 1 REVERSE PHASE (HYDROPHOBIC) Sorbent Part Numbers Sorbent Amount per Well, mg Endcapped C18 WSHCEC WSHCEC WSHCEC WSHCEC Endcapped C8 WSHCEC WSHCEC WSHCEC WSHCEC Endcapped C4 WSHCEC WSHCEC WSHCEC WSHCEC Sorbent Part Numbers Sorbent Amount per Well, mg Endcapped C2 WSHCEC WSHCEC WSHCEC WSHCEC Cyclohexyl WSHCYH WSHCYH WSHCYH WSHCYH Phenyl WSHPHY WSHPHY WSHPHY WSHPHY13 300, Inc Bartram Rd Bristol, PA USA

69 96 DEEP WELL PLATES IDEAL FOR ALL SPE APPLICATIONS AND HIGH THROUGHPUT SCREENING NORMAL PHASE (HYDROPHILIC) Sorbent Part Numbers Sorbent Amount per Well, mg Silica WSHSIL WSHSIL WSHSIL WSHSIL Pharma-Sil WSHPHSIL WSHPHSIL WSHPHSIL WSHPHSIL High Surface Activity Silica WSHHSSIL WSHHSSIL WSHHSSIL WSHHSSIL Diol WSHDOL WSHDOL WSHDOL WSHDOL Cyanopropyl WSHCNP WSHCNP WSHCNP WSHCNP Florisil mesh WSHFLS WSHFLS WSHFLS WSHFLS Florisil mesh, Grade A WSHFLSA WSHFLSA WSHFLSA WSHFLSA Alumina, Acidic WSHALA WSHALA WSHALA WSHALA Alumina, Neutral WSHALN WSHALN WSHALN WSHALN Alumina, Basic WSHALB WSHALB WSHALB WSHALB ION EXCHANGE (ANION) Sorbent Part Numbers Sorbent Amount per Well, mg Aminopropyl WSHNAX WSHNAX WSHNAX WSHNAX PSA ( N-2 Aminoethyl ) WSHPSA WSHPSA WSHPSA WSHPSA Diethylamino WSHDAX WSHDAX WSHDAX WSHDAX Quaternary Amine WSHQAX WSHQAX WSHQAX WSHQAX Polyimine WSHPAX WSHPAX WSHPAX WSHPAX ION EXCHANGE (CATION) Sorbent Part Numbers Sorbent Amount per Well, mg Benzenesulfonic Acid WSHBCX WSHBCX WSHBCX WSHBCX Benzenesulfonic Acid High Load WSHBCX1HL WSHBCX1HL WSHBCX1HL WSHBCX1HL Carboxylic Acid WSHCCX WSHCCX WSHCCX WSHCCX Propylsulfonic Acid WSHPCX WSHPCX WSHPCX WSHPCX Tri-Acetic Acid WSHTAX WSHTAX WSHTAX WSHTAX , Inc Bartram Rd Bristol, PA USA

70 96 DEEP WELL PLATES IDEAL FOR ALL SPE APPLICATIONS AND HIGH THROUGHPUT SCREENING 70 COPOLYMERIC (MULTIFUNCTIONAL PHASES) Sorbent Part Numbers Sorbent Amount per Well, mg Aminopropyl + C8 WSHNAX WSHNAX WSHNAX WSHNAX Quaternary Amine + C8 WSHQAX WSHQAX WSHQAX WSHQAX Carboxylic Acid + C8 WSHCCX WSHCCX WSHCCX WSHCCX Propylsulfonic Acid + C8 WSHPCX WSHPCX WSHPCX WSHPCX Benzenesulfonic Acid + C8 WSHBCX WSHBCX WSHBCX WSHBCX Cyanopropyl + C8 WSHCNP WSHCNP WSHCNP WSHCNP Cyclohexyl + C8 WSHCYH WSHCYH WSHCYH WSHCYH Diol + C18 WSHDOL WSHDOL WSHDOL WSHDOL POLYMERIC RESIN Sorbent Part Numbers Sorbent Amount per Well, mg DBX Benzenesulfonic Acid + C8 WSHDBX WSHDBX DVB Polystyrene Divinylbenzene C18 Reverse Phase C18 WSHDVB WSHDVB WSHC WSHC BCX Benzenesulfonic Acid WSHBCX WSHBCX QAX Quaternary Amine CCX Carboxlic Acid WSHQAX WSHQAX WORCCX WSHCCX406 60, Inc Bartram Rd Bristol, PA USA

71 48 DEEP WELL PLATES IDEAL FOR ALL SPE APPLICATIONS AND HIGH THROUGHPUT SCREENING Over 40 different sorbent chemistries Method development formats Custom packing and manufacturing Competitive pricing Full service technical support Satisfaction guaranteed Up to a 5mL sample volume per well Compatible with Robotic and Liquid Handling Systems Including: Advanced Chemtech, Beckman, Bohdan, Gilson, Hamilton, Packard, Sagian, Tecan, Tomtec, Zinser, Zymark 48 DEEP WELL FILTER PLATES Description Part Number Units Empty 48 deep well plate with one frit inserted WIM481F 1 Loose 48 deep well plate frits FR10481P deep well collection plate WIM48CP 1 REVERSE PHASE (HYDROPHOBIC) Sorbent Part Numbers Sorbent Amount per Well, mg Endcapped C18 WIMCEC WIMCEC WIMCEC WIMCEC181M 1000 Endcapped C8 WIMCEC WIMCEC WIMCEC WIMCEC081M 1000 Endcapped C4 WIMCEC WIMCEC WIMCEC WIMCEC041M 1000 Sorbent Part Numbers Sorbent Amount per Well, mg Endcapped C2 WIMCEC WIMCEC WIMCEC WIMCEC021M 1000 Cyclohexyl WIMCYH WIMCYH WIMCYH WIMCYH1M 1000 Phenyl WIMPHY WIMPHY WIMPHY WIMPHY1M , Inc Bartram Rd Bristol, PA USA

72 48 DEEP WELL PLATES IDEAL FOR ALL SPE APPLICATIONS AND HIGH THROUGHPUT SCREENING 72 NORMAL PHASE (HYDROPHILIC) Sorbent Part Numbers Sorbent Amount per Well, mg Silica WIMSIL WIMSIL WIMSIL WIMSIL1M 1000 Pharma-Sil WIMPHSIL WIMPHSIL WIMPHSIL WIMPHSIL1M 1000 High Surface Activity Silica WIMHSSIL WIMHSSIL WIMHSSIL WIMHSSIL1M 1000 Diol WIMDOL WIMDOL WIMDOL WIMDOL1M 1000 Cyanopropyl WIMCYN WIMCYN WIMCYN WIMCYN1M 1000 Florisil mesh WIMFLS WIMFLS WIMFLS WIMFLS1M 1000 Florisil mesh, Grade A WIMFLSA WIMFLSA WIMFLSA WIMFLSA1M 1000 Alumina, Acidic WIMALA WIMALA WIMALA WIMALA1M 1000 Alumina, Neutral WIMALN WIMALN WIMALN WIMALN1M 1000 Alumina, Basic WIMALB WIMALB WIMALB WIMALB1M 1000 ION EXCHANGE (ANION) Sorbent Part Numbers Sorbent Amount per Well, mg Aminopropyl WIMNAX WIMNAX WIMNAX WIMNAX1M 1000 PSA ( N-2 Aminoethyl ) WIMPSA WIMPSA WIMPSA WIMPSA1M 1000 Diethylamino WIMDAX WIMDAX WIMDAX WIMDAX1M 1000 Quaternary Amine WIMQAX WIMQAX WIMQAX WIMQAX1M 1000 Polyimine WIMPAX WIMPAX WIMPAX WIMPAX1M 1000 ION EXCHANGE (CATION) Sorbent Part Numbers Sorbent Amount per Well, mg Benzenesulfonic Acid WIMBCX WIMBCX WIMBCX WIMBCX1M 1000 Benzenesulfonic Acid High Load WIMBCXH1L WIMBCXH1L WIMBCXH1L WIMBCXH1L1M 1000 Carboxylic Acid WIMCCX WIMCCX WIMCCX WIMCCX1M 1000 Propylsulfonic Acid WIMPCX WIMPCX WIMPCX WIMPCX1M 1000 Tri-Acetic Acid WIMTAX WIMTAX WIMTAX15 500, Inc Bartram Rd Bristol, PA USA

73 48 DEEP WELL PLATES IDEAL FOR ALL SPE APPLICATIONS AND HIGH THROUGHPUT SCREENING COPOLYMERIC (MULTIFUNCTIONAL PHASES) Sorbent Part Numbers Sorbent Amount per Well, mg Aminopropyl + C8 WIMNAX WIMNAX WIMNAX WIMNAX2M 1000 Quaternary Amine + C8 WIMQAX WIMQAX WIMQAX WIMQAX2M 1000 Carboxylic Acid + C8 WIMCCX WIMCCX WIMCCX WIMCCX2M 1000 Propylsulfonic Acid + C8 WIMPCX WIMPCX WIMPCX WIMPCX2M 1000 Benzenesulfonic Acid + C8 WIMBCX WIMBCX WIMBCX WIMBCX2M 1000 Cyanopropyl + C8 WIMCNP WIMCNP WIMCNP WIMCNP2M 1000 Cyclohexyl + C8 WIMCYH WIMCYH WIMCYH WIMCYH2M 1000 Diol + C18 WIMDOL WIMDOL WIMDOL WIMDOL3M 1000 POLYMERIC RESIN Sorbent Part Numbers Sorbent Amount per Well, mg DBX Benzenesulfonic Acid + C8 WIMDBX WIMDBX WIMDBX WIMDBX4M 1000 DVB Polystyrene Divinylbenzene WIMDVB WIMDVB WIMDVB WIMDVB4M 1000 C18 Reverse Phase C18 WIMC WIMC WIMC WIMC184M 1000 BCX Benzenesulfonic Acid WIMBCX WIMBCX WIMBCX WIMBCX4M 1000 QAX Quaternary Amine WIMQAX WIMQAX WIMQAX WIMQAX4M , Inc Bartram Rd Bristol, PA USA

74 CT FLASH CHROMATOGRAPHY COLUMNS 74 Compatible with common Flash Systems including Biotage * Ensures high yield separations Observe reproducible results Guaranteed not to leak Optimized performance * Biotage is a trademark of Dyax Corporation, Inc Bartram Rd Bristol, PA USA

75 FLASH CHROMATOGRAPHY COLUMNS ULTRA FLASH I COMPATIBLE WITH BIOTAGE SILICA Silica Quantity Part Number Size Bed Weight per package FUSIL12S-20 12S 4g 20 FUSIL12S S 4g 100 FUSIL12M-20 12M 8g 20 FUSIL12M M 8g 100 FUSIL40S-12 40S 40g 12 FUSIL40S S 40g 144 FUSIL40M-12 40M 90g 12 FUSIL40M M 90g 144 FUSIL40L-12 40L 120g 12 FUSIL65M-6 65M 300g 6 C18 Silica Quantity Part Number Size Bed Weight per package FEC1812S S 4g 100 FEC1812M-20 12M 8g 20 FEC1812M M 8g 100 FEC1840S-12 40S 40g 12 FEC1840S S 40g 144 FEC1840M-12 40M 90g 12 FEC1840M M 90g 144 FEC1840L-12 40L 120g 12 FEC1865M-6 65M 300g 6 75 ULTRA FLASH II COMPATIBLE WITH BIOTAGE FLASHMASTER SILICA Silica Quantity Part Number Size Bed Weight per package MUSIL12M15 15mL 2g 20 MUSIL15M25 25mL 5g 20 MUSIL110M25 25mL 10g 20 MUSIL110M75 75mL 10g 16 MUSIL120M75 75mL 20g 16 MUSIL125M mL 25g 8 MUSIL150M mL 50g 8 MUSIL170M mL 70g 8 C18 Silica Quantity Part Number Size Bed Weight per package MEC1812M15 15mL 2g 20 MEC1815M25 25mL 5g 20 MEC18110M25 25mL 10g 20 MEC18110M75 75mL 10g 16 MEC18120M75 75mL 20g 16 MEC18125M mL 25g 8 MEC18150M mL 50g 8 MEC18170M mL 75g 8, Inc Bartram Rd Bristol, PA USA

76 SOLID PHASE EXTRACTION PRODS CATALOG DCN FORENSICS CLEAN SCREEN FASt Page 16, Inc Bartram Rd Bristol, PA USA Feedback on our new catalogue? Let us know what you think: