Indice generale. Anticorpi monoclonali e Kit Diagnostici (Bio X) 5. Kit PCR per Veterinaria (intron) 21

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1 Indice generale Anticorpi monoclonali e Kit Diagnostici (Bio X) 5 Kit PCR per Veterinaria (intron) 21 e-myco PCR Detection Kit determinazione dei micoplasmi nelle colture cellulari 24 Kit di estrazione rapida di acidi nucleici (Omega Bio-Tek) 29 3

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3 Bio X Kit Diagnostici 6 Bovine e altri kit 6 Fish 11 Miscellaneous 13 Strips 15 Anticorpi monoclonali 16 Bovine 16 Porcine 18 Equine 18 Rabbit 18 Fish 19 5

4 Bio X: Bovine e altri kit Respiratory diseases (serological diagnosis) BIO-K027 BHV-1 (IBR) threshold 96 Test BIO-K132 BHV-1 (IBR) seroconversion 96 Test BIO-K238 BHV-1 (IBR) ready to use 96 Test BIO-K004 BVDV antibody 96 Test BIO-K230 BVDV antibody (competition) 192 Test BIO-K061 BRSV 96 Test BIO-K241 BRSV ready to use 96 Test BIO-K062 Parainfluenza 3 (PI3) 96 Test BIO-K239 Parainfluenza 3 (PI3) ready to use 96 Test BIO-K063 Adenovirus 3 (ADENO 3) 96 Test BIO-K162 Mycoplasma bovis 96 Test BIO-K260 Mycoplasma bovis (seroconversion) 96 Test BIO-K028 Respiratory pentavalent kit (BHV-1, BVDV, BRSV, PI3, ADENO 3) 32 x 5 Test BIO-K146 Respiratory trivalent RPA (BRSV, PI3, ADENO 3) 48 x 3 Test BIO-K243 Respiratory trivalent Sero RPM (BRSV, PI3, Mycoplasma bovis) 48 x 3 Test Respiratory diseases (antigenic diagnosis) BIO-K189 Pulmotest BHV-1 96 Test BIO-K190 Pulmotest BVDV 96 Test BIO-K187 Pulmotest PI3 96 Test BIO-K188 Pulmotest BRSV 96 Test BIO-K121 Pulmotest Mycoplasma bovis 24 Test BIO-K199 Pulmotest ADENO 3 96 Test BIO-K191 Pulmotest Tetra (BHV-1, BVDV, BRSV, PI3) 24 x 4 Test Digestive diseases (serological diagnosis) BIO-K126 Rotavirus (sero blocking) 192 BIO-K127 Coronavirus (sero blocking) 192 BIO-K128 E. coli F5 (K99) (sero blocking) 192 Digestive diseases (antigenic diagnosis) BIO-K067 Rotavirus 96 Test BIO-K068 Coronavirus 96 Test BIO-K069 E. coli F5 (K99) 96 Test BIO-K070 Cryptosporidium parvum 96 Test BIO-K071 Digestive kit (Rota, Corona, E. coli F5 (K99), Crypto) 24 x 4 Test BIO-K083 Duo Digestive (Rota, Corona) 48 x 2 Test BIO-K151 Easy-Digest (4 Ab on the same well) (Rota, Corona, E. coli F5 (K99), Crypto) 96 Test 6

5 Bio X: Bovine e altri kit Abortion BIO-K132 BHV-1 (IBR) seroconversion 96 Test BIO-K004 BVDV antibody 96 Test BIO-K066 BHV-4 96 Test BIO-K072 Abortion kit (BHV-1, BVDV, BHV-4) 48 x 3 Test BIO-K192 Neospora caninum (indirect sero) P38 96 Test 7

6 Bio X: Bovine e altri kit BVDV antigen detection BIO-K082 BVDV Antigen (NS3 on leucocytes) ELISA Kit 192 Test BIO-K258 BVDV Antigen one step ELISA Kit 192 Test Clostridium perfringens (serological diagnosis) BIO-K221 Clostridium perfringens Alpha toxin (sero) 96 Test BIO-K222 Clostridium perfringens Epsilon toxin (sero) 96 Test Clostridium perfringens (antigen detection) BIO-K084 Alpha toxin (Ag) 96 Test BIO-K094 Beta toxin (Ag) 96 Test BIO-K085 Epsilon toxin (Ag) 96 Test BIO-K086 Clostridium perfringens (Ag) 96 Test BIO-K095 Enterotoxaemia (toxinotyping α, β, ε, C. perf.) 24 Test Fasciola hepatica BIO-K201 Fasciola Hepatica (antigen) 96 Test BIO-K211 Fasciola hepatica (serology) (2) 96 Test Miscellaneous BIO-K093 Bovine Gamma Interferon ELISA Kit 80 x 2 Test BIO-K165 Bovine Immunoglobulin ELISA Kit (quant) 80 x 2 Test BIO-K158 Bovine Lactoferrin ELISA Kit 80 x 2 Test 8

7 Bio X: Bovine e altri kit, descrizioni tecniche ELISA Kit for IBR (Bovine Herpes Virus 1) BIO-K027 ELISA kit for the serodiagnosis of infectious bovine rhinotracheitis (IBR). This serological test allows testing 96 sera in only 2 hours. The kit contains threshold control for sera. ELISA Kit for BVDV (mucosal disease) BIO-K004 ELISA kit for the serological diagnosis of BVDV in cattle. This kit allows testing 96 sera in only 2 hours. A positive control serum is included in the kit in order to determine the positivity level of unknown samples. ELISA Kit for BRSV (Bovine Respiratory Syncytial Virus) BIO-K239 ELISA kit for the serological diagnosis of BRSV in cattle. This kit allows testing 96 sera in only 2 hours. A positive control serum is included in the kit in order to determine the positivity level of unknown samples. ELISA Kit for PI3 (Bovine Parainfluenza Virus 3) BIO-K239 ELISA kit for the serological diagnosis of PI3 in cattle. This kit allows testing 96 sera in only 2 hours. A positive control serum is included in the kit in order to determine the positivity level of unknown samples. ELISA Kit for Bovine Adenovirus 3 BIO-K063 ELISA kit for the serological diagnosis of adenovirus 3 in cattle. This kit allows testing 96 sera in only 2 hours. A positive control serum is included in the kit in order to determine the positivity level of unknown samples. ELISA Respiratory Pentavalent Kit BIO-K028 ELISA kit for the serological diagnosis of acute respiratory disease in cattle. Most of the more frequent respiratory pathogenic agents are included in this kit (IBR, BRSV, BVDV, PI3, Adenovirus 3 ). The kit allows to screen 32 sera against these 5 pathogens. A positive control serum is included in the kit in order to determine the positivity level of unknown samples. ELISA Kit for BHV-4 (Bovine Herpes Virus 4) BIO-K066 ELISA kit for the serological diagnosis of BHV-4 in cattle. This kit allows testing 96 sera in only 2 hours. A positive control serum is included in the kit in order to determine the positivity level of unknown samples. ELISA Abortion Kit BIO-K072 ELISA kit for the serological diagnosis of IBR, BHV-4 and BVDV. This kit allows testing 48 samples in only 2 hours. A positive control serum is included in the kit in order to determine the positivity level of unknown samples for each agent. ELISA Kit for Rotavirus (sero blocking) BIO-K126 ELISA kit for the antigenic diagnosis of rotavirus in stool from cattle. This kit allows testing 96 samples in only 2 hours. A positive control is included in the kit in order to determine the positivity level of unknown samples. ELISA Kit for Coronavirus (sero blocking) BIO-K127 ELISA kit for the antigenic diagnosis of coronavirus in stool from cattle. This kit allows testing 96 samples in only 2 hours. A positive control is included in the kit in order to determine the positivity level of unknown samples. ELISA Kit for K99 factor of E. Coli (sero blocking) BIO-K128 ELISA kit for the antigenic diagnosis of E. Coli K99 factor in stool from cattle. This kit allows testing 96 samples in only 2 hours. A positive control is included in the kit in order to determine the positivity level of unknown samples. ELISA Kit for Cryptosporidium Parvum BIO-K070 ELISA kit for the antigenic diagnosis of Cryptosporidium parvum in stool from cattle. This kit allows testing 96 samples in only 2 hours. A positive control is included in the kit in order to determine the positivity level of unknown samples. 9

8 Bio X: Bovine e altri kit, descrizioni tecniche ELISA Digestive Kit BIO-K071 ELISA kit for the antigenic diagnosis of rotavirus, coronavirus, K99 factor of E. Coli and Cryptosporidium parvum in stool from cattle. This kit allows testing 24 samples in only 2 hours against the 4 pathogens. A positive control for each agent is included in the kit in order to determine the positivity level of unknown samples. ELISA DUO Digestive Kit BIO-K083 ELISA kit for the antigenic diagnosis of rotavirus and coronavirus in stool from cattle. This kit allows testing 48 samples in only 2 hours against the 2 pathogens. A positive control for each agent is included in the kit in order to determine the positivity level of unknown samples. ELISA Kit for BVDV BIO-K082 ELISA kit for the antigenic diagnosis of BVDV and Mucosal disease. This kit allows testing 192 samples in only 2 hours. A positive control antigen is included in the kit in order to determine the positivity level of unknown samples. ELISA Kit for Alpha Toxin from Clostridium Perfringens BIO-K084 ELISA kit for the detection of Clostridium perfringens Alpha toxin in biological fluids or culture supernatants. The kit uses polyclonal antibodies. ELISA Kit for Beta Toxin from Clostridium Perfringens BIO-K094 ELISA kit for the detection of Clostridium perfringens Beta toxin in biological fluids or culture supernatants. The kit uses polyclonal and monoclonal antibodies. ELISA Kit for Epsilon Toxin from Clostridium Perfringens BIO-K085 ELISA kit for the detection of Clostridium perfringens Epsilon toxin in biological fluids or culture supernatants. The kit uses polyclonal and monoclonal antibodies. ELISA Kit for Clostridium Perfringens Direct Detection BIO-K086 Some strains of Clostridium perfringens don t produce any detectable alpha toxin in vitro. It was therefore impossible to determine their toxinotype by the classical seroneutralisation test. Even with an alpha toxin ELISA kit these strains remain sometimes alpha negative. The C. perfringens detection kit contains a monoclonal antibody specific for a constitutional protein of C. perfringens. ELISA Kit for Enterotoxaemia BIO-K095 Kit for the detection of alpha-, beta- and epsilon- toxin of Clostridium perfringens as well as semi-quantitative detection of the bacterium itself, in biological samples and in culture supernatants. ELISA Kit for Bovine Interferon Gamma Kit for the quantitative determination of bovine gamma Interferon by ELISA. BIO-K

9 ELISA Kits Bio X: Fish kit BIO-K159 Aeromonas salmonicida 96 Test BIO-K181 VHS 96 Test BIO-K198 IHN 96 Test BIO-K194 Duo VHS-IHN 48 x 2 Test BIO-K215 IPN (Ag) 96 Test BIO-K220 SVC (Ag) 96 Test BIO-FLUO Kits BIO-K006 VHS (Viral Haemorrhagic Septicaemia) 24 x 2 Test BIO-K008 IHN (Infectious Haematopoietic Necrosis) 24 x 2 Test BIO-K213 IPN (Infectious Pancreatic Necrosis) 24 x 2 Test BIO-K196 SVC (Spring Viraemia of Carp) 24 x 2 Test BIO-PEROX Kits BIO-K007 VHS 24 x 2 Test BIO-K009 IHN 24 x 2 Test BIO-K214 IPN (2) 24 x 2 Test BIO-K197 SVC 24 x 2 Test 11

10 Bio X: Fish kit, descrizioni tecniche BIO-FLUO VHS BIO-K006 Immunofluorescent assay for the detection of viral haemorrhagic septicaemia (VHS) virus in cell cultures. This kit is sufficient to detect two 24 wells microplates and it is based on monoclonal antibodies technology. This kit does not detect a few VHS strains. BIO-PEROX VHS BIO-K007 Immunoenzyme assay for the detection of viral haemorrhagic septicaemia (VHS) virus in cell cultures. This kit is sufficient to detect two 24 wells microplates and it is based on monoclonal antibodies technology.. This kit does not detect a few VHS strains. BIO-FLUO IHN BIO-K008 Immunofluorescent assay for the detection of infectious haematopoietic necrosis (IHN) virus in cell cultures. This kit is sufficient to detect two 24 wells microplates and it is based on monoclonal antibodies technology. Until now, this kit has not detected only one IHN strain. BIO-PEROX IHN BIO-K009 Immunoenzyme assay for the detection of infectious haematopoietic necrosis (IHN) virus in cell cultures. Until now, this kit has not detected only one IHN strain. BIO-FLUO IPN BIO-K213 Immunofluorescent assay for the detection of infectious pancreatic necrosis (IPN) virus in cell cultures. This kit is sufficient to detect two 24 wells microplates and it is based on monoclonal antibodies technology BIO-PEROX IPN BIO-K214 Immunoenzyme assay for the detection of infectious pancreatic necrosis (IPN) virus in cell cultures. This kit is suff cient to detect two 24 wells microplates and it is based on monoclonal antibodies technology. BIO-FLUO SVC BIO-K196 Immunofluorescent assay for the detection of spring viraemia of carp (SVC) virus in cell cultures. This kit is sufficient to detect two 24 wells microplates and it is based on monoclonal antibodies techno- logy. BIO-PEROX SVC BIO-K197 Immunoenzyme assay for the detection of spring viraemia of carp (SVC) virus in cell cultures. This kit is sufficient to detect two 24 wells microplates and it is based on monoclonal antibodies technology. 12

11 Clostridium perfringens (antigen detection) Bio X: Miscellaneous kit BIO-K084 Alpha toxin (Ag) ELISA Kit 96 Test BIO-K094 Beta toxin (Ag) ELISA Kit 96 Test BIO-K085 Epsilon toxin (Ag) ELISA Kit 96 Test BIO-K086 Clostridium perfringens (Ag) ELISA Kit 96 Test BIO-K095 Enterotoxaemia (toxinotyping α, β, ε, C. perf.) (Ag) ELISA Kit 24 Test Anabolics Kits: Anabolic Steroids BIO-K207 Zeranol ELISA Kit* 96 Test BIO-K Nortestosterone ELISA Kit* 96 Test BIO-K209 b-trenbolone ELISA Kit* 96 Test BIO-K210 Diethylstilbestrol (DES) ELISA Kit* 96 Test BIO-K254 Boldenone ELISA Kit* 96 Test BIO-K255 Corticosteroids ELISA Kit* 96 Test Anabolics Kits: Beta2 Agonists BIO-K164 Clenbuterol ELISA Kit* 96 Test BIO-K076-1 b2 Agonist (quantitative) ELISA Kit* 96 Test BIO-K076-2 b2 Agonist (qualitative) ELISA Kit* 96 Test BIO-K076-3 b2 Agonist (tube) ELISA Kit* 25 Test Anabolics Kits: Other Compounds BIO-K206 Chloramphenicol ELISA Kit* 96 Test BIO-K090 Isoxsuprine ELISA Kit* 96 Test BIO-K256 Medroxyprogesterone ELISA Kit* 96 Test Anabolics Kits: Chromatographie column BIO-K212 Multi-Residual Column IAC* 1 Antibiotics and Sulfur Drugs detection kits BIO-K033-1 Total Antibiotic (plate) milk & meat* 288 Test BIO-K033-2 Total Antibiotic (tube) milk & meat* 96 Test BIO-K033-3 Total Antibiotic & Sulphonamide (plate) milk* 288 Test BIO-K033-4 Total Antibiotic & Sulphonamide (tube) milk* 96 Test BIO-K257 CHROM-AB* 288 Test * su richiesta 13

12 Bio X: Miscellaneous kit, descrizioni tecniche ß Agonists Quantitative ELISA Kit BIO-K076-1 The ß agonists quantitative ELISA kit is an indirect competitive enzyme immunoassay for the quantitative analysis of tert-butylic ß-agonists in biological samples. The test is suitable for human, bovine and equine urines, sera and liver, for feeds, milk and milk-powder. No previous purification of samples is required. Detection limit: Clenbuterol: 0.6 ppb Bromobuterol: 0.5 ppb Mabuterol: 0.6 ppb Salbutamol: 2 ppb Mapenterol: 2 ppb Terbutaline: 3 ppb The kit contains 1 microplate ß Agonists Qualitative ELISA Kit BIO-K076-2 The ß agonists qualitative ELISA kit is an indirect competitive enzyme immunoassay for the qualitative analysis of tertbutylic ß-agonists in biological samples. The test is suitable for human, bovine and equine urines, sera and liver, for feeds, milk and milk-powder. No previous purification of samples is required. The test detects the following levels of ß-agonists in samples: Clenbuterol: 1 ppb Bromobuterol: 1 ppb Mabuterol: 1 ppb Salbutamol: 3 ppb Mapenterol: 3 ppb Terbutaline: 4 ppb The kit contains 1 microplate ß Agonists Qualitative ELISA Kit (test tubes) BIO-K076-3 The ß agonists qualitative ELISA kit is a qualitative visual assay for the detection of tert-butylic ß-agonists in biological samples. The test is suitable for human, bovine and equine urines, sera and liver, for feeds, milk and milk-powder. No previous purification of samples is required. Furthermore, for urines and sera, no laboratory equipment is required, so that a simple, rapid test can be performed also outside the laboratory. The test detects the following levels of ß- agonists in samples: Clenbuterol: 1 ppb Bromobuterol: 1 ppb Mabuterol: 1 ppb Salbutamol: 3 ppb Mapenterol: 3 ppb Terbutaline: 4 ppb The kit contains 25 coated tubes. Total Antibiotics (microplate test) BIOK033-1 It is a kit for the rapid detection of antimicrobial substances in milk and meat. The principle of the test is the inhibition of bacterial growth by antibiotic substances. The assay gives a qualitative answer. Due to its simplicity, short assay time, multiresiduality, this kit is very suitable for screening assays. No reagents or instruments are needed apart from a water bath (65 C). The kit is available in microtiter plates (3 plates, 8 wells strip). Total Antibiotics (test tube) BIOK033-2 It is a kit for the rapid detection of antimicrobial substances in milk and meat. The principle of the test is the inhibition of bacterial growth by antibiotic substances. The assay gives a qualitative answer. Due to its simplicity, short assay time, multiresiduality, this kit is very suitable for screening assays. No reagents or instruments are needed apart from a water bath (65 C). The kit is available in test tubes (96 tests). Total Antibiotics Sulphonamide (microplate test) BIO-K033-3 It is a kit for the rapid detection of antimicrobial substances in milk. The principle of the test is the inhibition of bacterial growth by antibiotic or sulphonamide substances. The assay gives a qualitative answer. Due to its simplicity, short assay time, multiresiduality, this kit is very suitable for screening assays. No reagents or instruments are needed apart from a water bath (65 C). The kit is available in microtiter plates (3 plates, 8 wells strip). Total Antibiotics Sulphonamide (test tube) BIO-K033-4 It is a kit for the rapid detection of antimicrobial substances in milk. The principle of the test is the inhibition of bacterial growth by antibiotic or sulphonamide substances. The assay gives a qualitative answer. Due to its simplicity, short assay time, multiresiduality, this kit is very suitable for screening assays. No reagents or instruments are needed apart from a water bath (65 C). The kit is available in test tubes (96 tests). 14

13 Digestive diseases (antigen detection) Bio X: Strips kit BIO-K152 Rotavirus Strips Kit 10 Test BIO-K153 Coronavirus Strips Kit 10 Test BIO-K154 E.Coli F5 (K99) Strips Kit 10 Test BIO-K155 Cryptosporidium Parvum Strips Kit 10 Test BIO-K156 Tetrastrips (Rota, Corona, E. Coli F5 (K99), Cryptosporidium Parvum) Strips Kit 4 x 5 Test Enterotoxemia (antigen detection) BIO-K170 Alpha Toxin Strips Kit 10 Test BIO-K176 Epsilon Toxin Strips Kit 10 Test BIO-K195 Clostridium Perfringens Strips Kit 10 Test Respiratory disease BIO-K253 BRSV Strips Kit 10 Test BIO-K342 BHV1 Ag Strips Kit 10 Test 15

14 Bio X: Bovine anticorpi Respiratory diseases (monoclonal) BIO-292 Anti-ADENO 3 0,5 ml (dilution 1/20) BIO-289 Anti-BHV-1 (IBR) 0,5 ml (dilution 1/20) BIO-031 Anti-BRSV 0,5 ml (dilution 1/20) BIO-295 Anti-BVDV (NS3) 0,5 ml (dilution 1/20) BIO-297 Anti-Mycoplasma bovis 0,5 ml (dilution 1/20) BIO-290 Anti-PI3 0,5 ml (dilution 1/20) Digestive diseases (monoclonal) BIO-288 Anti-Coronavirus 0,5 ml (dilution 1/20) BIO-294 Anti-Cryptosporidium parvum 0,5 ml (dilution 1/20) BIO-293 Anti-E. coli F5 (K99) 0,5 ml (dilution 1/20) BIO-287 Anti-Rotavirus 0,5 ml (dilution 1/20) Abortion (monoclonal) BIO-289 Anti-BHV-1 (IBR) 0,5 ml (dilution 1/20) BIO-279 Anti-BHV-4 0,5 ml (dilution 1/20) BIO-295 Anti-BVDV (NS3) 0,5 ml (dilution 1/20) BIO-319 Anti-Neospora caninum 0,5 ml (dilution 1/20) Respiratory diseases (monoclonal) BIO-039 FITC Anti-ADENO 3 0,5 ml (dilution 1/20) BIO-026 FITC Anti-BHV-1 (IBR) 0,5 ml (dilution 1/20) BIO-032 FITC Anti-BRSV 0,5 ml (dilution 1/20) BIO-316 FITC Anti-BVDV 0,5 ml (dilution 1/20) BIO-274 FITC Anti-Mycoplasma bovis 0,5 ml (dilution 1/20) BIO-030 FITC Anti-PI3 0,5 ml (dilution 1/20) Digestive diseases (monoclonal) BIO-023 FITC Anti-Coronavirus 0,5 ml (dilution 1/20) BIO-073 FITC Anti-Cryptosporidium parvum 0,5 ml (dilution 1/20) BIO-062 FITC Anti-E. coli F5 (K99) 0,5 ml (dilution 1/20) BIO-020 FITC Anti-Rotavirus 0,5 ml (dilution 1/20) Abortion (monoclonal) BIO-026 FITC Anti-BHV-1 (IBR) 0,5 ml (dilution 1/20) BIO-286 FITC Anti-BHV-4 0,5 ml (dilution 1/20) BIO-316 FITC Anti-BVDV 0,5 ml (dilution 1/20) BIO-318 FITC Anti-Neospora caninum 0,5 ml (dilution 1/20) 16

15 Secondary Antibodies Bio X: Bovine anticorpi BIO-305 FITC Rabbit Anti-mouse Ig 0,5 ml (dilution 1/20) BIO-343 FITC Rabbit Anti-goat Ig 0,5 ml (dilution 1/20) Consulta anche il catalogo completo degli anticorpi secondari della Jackson ImmunoResearch disponibile on line al sito: 17

16 Bio X: Altri anticorpi Porcine: Unconjugated reagents (monoclonal) BIO-275 Anti-CSFV (BFAV/KSP/D13/01) 0,5 ml (dilution 1/20) BIO-276 Anti-PRRSV (EUR/USA) 0,5 ml (dilution 1/20) BIO-277 Anti-Pseudorabies (Aujeszky) SuHV-1 0,5 ml (dilution 1/20) BIO-323 Anti-Lawsonia intracellularis 0,5 ml (dilution 1/20) Porcine: Immunofluorescence reagents (monoclonal) BIO-272 FITC Anti-CSFV (Hog Cholera) BFAV B 360 0,5 ml (dilution 1/20) BIO-268 FITC Anti-PRRSV 0,5 ml (dilution 1/20) BIO-265 FITC Anti-Pseudorabies (Aujeszky) SuHV-1 0,5 ml (dilution 1/20) Equine: Unconjugated reagents (monoclonal) BIO-278 Anti-EAV (Equine Arteritis Virus) 0,5 ml (dilution 1/20) Equine: Immunofluorescence reagents (monoclonal) BIO-245 FITC Anti-EAV (Equine Arteritis Virus) 0,5 ml (dilution 1/20) Rabbit: Unconjugated reagents (monoclonal) BIO-315 Anti-RHDV (Rabbit Haemorrhagic Disease) 0,5 ml (dilution 1/20) Rabbit: Immunofluorescence reagents (monoclonal) BIO-314 FITC Anti-RHD (Rabbit Haemorrhagic Disease) 0,5 ml (dilution 1/20) Secondary Antibodies and their conjugates BIO-296 Anti-Bovine IgG1 0,5 ml (dilution 1/20) BIO-300 Anti-Bovine IgM 0,5 ml (dilution 1/20) BIO-328 Anti-Bovine IgG2 0,5 ml (dilution 1/20) BIO-305 FITC Rabbit Anti-mouse Ig 0,5 ml (dilution 1/20) BIO-343 FITC Rabbit Anti-goat Ig 0,5 ml (dilution 1/20) BIO-269 Perox Rabbit Anti-mouse Ig 0,5 ml (dilution 1/20) BIO-342 Perox Rabbit Anti-goat Ig 0,5 ml (dilution 1/20) Consulta anche il catalogo completo degli anticorpi secondari della Jackson ImmunoResearch disponibile on line al sito: 18

17 Unconjugated reagents (monoclonal) Bio X: Fish anticorpi BIO-282 Anti-VHS (IP5B11) Anti-N protein 0,5 ml (dilution 1/20) BIO-285 Anti-IHN (136-3) Anti-N protein 0,5 ml (dilution 1/20) BIO-312 Anti-IHN (34D3) Anti-N protein 0,5 ml (dilution 1/20) BIO-331 Anti-SVC (Spring Viraemia of Carp) 0,5 ml (dilution 1/20) BIO-334 Anti-KHV (Koi Herpes Virus) 0,5 ml (dilution 1/20) BIO-337 Anti-ISA (Infectious Salmon Anaemia) 0,5 ml (dilution 1/20) Unconjugated reagents (policlonal) BIO-341 Anti-IPN (Infectious Pancreatic Necrosis) (2) 0,5 ml (dilution 1/20) Immunofluorescence reagents (monoclonal) BIO-281 FITC Anti-VHS (IP5B11) 0,5 ml (dilution 1/20) BIO-284 FITC Anti-IHN (136-3) 0,5 ml (dilution 1/20) BIO-311 FITC Anti-IHN (34D3) 0,5 ml (dilution 1/20) Secondary Antibodies and their conjugates BIO-305 FITC Rabbit Anti-mouse Ig 0,5 ml (dilution 1/20) BIO-343 FITC Rabbit Anti-goat Ig 0,5 ml (dilution 1/20) BIO-269 Perox Rabbit Anti-mouse Ig 0,5 ml (dilution 1/20) BIO-342 Perox Rabbit Anti-goat Ig 0,5 ml (dilution 1/20) Consulta anche il catalogo completo degli anticorpi secondari della Jackson ImmunoResearch disponibile on line al sito: 19

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19 intron Kit PCR per Veterinaria 22 Canine Feline Avian Porcine Shirmp e-myco PCR Detection Kit determinazione dei micoplasmi nelle colture cellulari 24 Kit PCR, descrizione tecnica 25 Related Products 26 DNA/RNA extration PCR/RT-PCR pre-mix Others 21

20 intron: Kit PCR Detection Kits for Canine Infectious Disease Codice Prodotto Descrizione Tipo Confezione INT-D10230 VeTeK CPV Detection kit Canine parvovirus type 1 and type 2 PCR 96 Test INT-D10240 VeTeK CAdV Detection kit Canine adenovirus PCR 96 Test INT-D10250 VeTeK CDV Detection kit Canine distemper virus nested-pcr 48 Test INT-D10260 VeTeK CCoV Detection kit Canine coronavirus RT-PCR 96 Test INT-D10270 VeTeK CPIV Detection kit Canine parainfluenza virus RT-PCR 96 Test INT-D10280 VeTeK BAB-C Detection kit Babesia canis & B. gibsoni PCR 96 Test INT-D10290 VeTeK HBN-C Detection Kit Micoplasma haemocanis PCR 96 Test INT-D10300 VeTeK EHR Detection Kit Ehrlichia sp. PCR 96 Test INT-D10310 VeTeK RV Detection kit Rabies virus RT-PCR 96 Test INT-D10320 VeTeK HW Detection kit Dirofilaria immitis PCR 96 Test INT-D10330 VeTeK CHV Detection kit Canine herpes virus PCR 96 Test INT-D10340 VeTeK GIA Detection kit Giardia sp. PCR 96 Test INT-D10350 VeTeK MYCO Detection kit Mycoplasma sp. PCR 96 Test INT-D10370 VeTeK LEPTO Detection kit Leptospira sp. PCR 96 Test INT-D10380 VeTeK SAL Detection kit Salmonella sp. PCR 96 Test INT-D10390 VeTeK CAMPY Detection kit Campylobacter jenuni PCR 96 Test INT-D10400 VeTeK CLOS Detection kit Clostridium perfringens PCR 96 Test INT-D10410 VeTeK RICK Detection kit Rickettsia rickettsii PCR 96 Test INT-D10420 VeTeK BRU Detection kit Brucella sp. PCR 96 Test Detection Kits for Feline Infectious Disease Codice Prodotto Descrizione Tipo Confezione INT-D20110 VeTeK FIV Detection kit Feline immunodeficiency virus PCR 96 Test INT-D20120 VeTeK FeLV Detection kit Feline leukemia virus RT-PCR 96 Test INT-D20130 VeTeK HBN-F Detection kit Mycoplasma haemofelis PCR 96 Test INT-D20140 VeTeK FPV Detection kit Feline panleukopenia PCR 96 Test INT-D20150 VeTeK FCoV Detection kit Feline Coronavirus or infectious peritonitis virus RT-PCR 50 Test INT-D20170 VeTeK CHLA Detection kit Chlamydia psittaci (Feline chlamydiosis) PCR 96 Test INT-D20180 VeTeK FHV Detection kit Feline herpes virus PCR 96 Test INT-D20190 VeTeK FCV Detection kit Feline calicivirus RT-PCR 96 Test INT-D20200 VeTeK TOXO Detection kit Toxoplasma gondii PCR 96 Test Detection Kits for Avian Infectious Disease Codice Prodotto Descrizione Tipo Confezione INT-D30160 VeTeK CAV Detection kit Chicken anemia virus PCR 96 Test INT-D30170 VeTeK APEC Detection kit Avian pathogenic Escherichia coli PCR 96 Test INT-D30180 VeTeK ALV Detection kit Avian leukosis virus PCR 96 Test INT-D30200 VeTeK MDV Detection kit Marek disease virus nested-pcr 48 Test INT-D30210 VeTeK ILTV Detection kit Infectious laryngotrachitis virus PCR 96 Test INT-D30220 VeTeK NDV Detection kit Newcastle disease virus (common & patho) RT-PCR 48 Test INT-D30230 VeTeK IBV Detection kit Infectious bronchitis virus RT-PCR 96 Test INT-D30240 VeTeK AIV Detection kit Avian influenza virus common type RT-PCR 96 Test INT-D30250 VeTeK AIV H5 Detection kit Avian influenza virus serotype H5 RT-PCR 96 Test INT-D30260 VeTeK AIV H7 Detection kit Avian influenza virus serotype H7 RT-PCR 96 Test INT-D30270 VeTeK AIV H9 Detection kit Avian influenza virus serotype H9 RT-PCR 96 Test INT-D30290 VeTeK IBDV Detection kit Infectious bursal disease virus RT-PCR 96 Test INT-D30330 VeTeK MS/MG Detection kit Avian mycoplasma (MS, MG) nested-pcr 24 Test 22

21 intron: Kit PCR Detection Kits for Porcine Infectious Disease Codice Prodotto Descrizione Tipo Confezione INT-D40130 VeTeK PAS Detection kit Pasteurella multocida PCR 96 Test INT-D40140 VeTeK PAS-A Detection kit Pasteurella multocida type A PCR 96 Test INT-D40150 VeTeK PAS-D Detection kit Pasteurella multocida type D PCR 96 Test INT-D40160 VeTeK BOR Detection kit Bordetella bronchiseptica PCR 96 Test INT-D40170 VeTeK MYCO-P Detection kit Mycoplasma hyopneumoniae PCR 96 Test INT-D40180 VeTeK PCV Detection kit Porcine circo virus PCR 96 Test INT-D40190 VeTeK PRRS Detection kit Porcine reproductive & respiratory syndrome virus RT-PCR 96 Test INT-D40200 VeTeK SIV Detection kit Swine influenza virus RT-PCR 96 Test INT-D40210 VeTeK TEG/PED Detection kit Transmissible gastroenteritis & Porcine epidimic diarrhea virus RT-PCR 96 Test INT-D40230 VeTeK PCV-2 Detection kit Porcine circovirus type 2 PCR 96 Test INT-D40240 VeTeK ADV Detection kit Aujeszky's disease virus PCR 96 Test Detection Kits for Shrimp Infectious Disease Codice Prodotto Descrizione Tipo Confezione INT-D50050 VeTeK VIBRIO Detection kit Vibrio sp. PCR 96 Test INT-D50060 VeTeK WSSV Detection kit White spot syndrome virus PCR 96 Test 23

22 intron: Related Products e-myco Caratteristiche: e-myco Mycoplasma PCR Detection Kit: per rilevare le contaminazioni da Mycoplasma Il Mycoplasma è un contaminante delle colture cellulari che, pur non danneggiando in maniera visibile la cellula, ne altera alcuni parametri fondamentali, quali ad esempio metabolismo, crescita, sintesi di DNA, RNA e proteine. Procedura: e-myco Mycoplasma Detection Kit 5x10 4 cells Bollitura (95, 5 min) Estrazione DNA genomico Sequenziamento dei Prodotti PCR Rileva la contaminazione da Mycoplasma Identifica la specie di Mycoplasma contaminante Risultati: Mycoplasma detection performed with e-myco Mycoplasma Detection kit using isolated genomic DNA of infected K562 cells. Template range: from 200 ng (lane1) to 6.3 pg (lane 16) Mycoplasma detection performed with e-myco Mycoplasma Detection kit using K562-infected cell lysates. Cell number range: 1x10 6 to 1x10 3 Vantaggi: Efficiente: Basato sull amplificazione per PCR delle sequenze conservate rrna 16S. Lo stato di contaminazione viene verificato con elettroforesi dei frammenti amplificati (270bp) Ampio spettro: rileva la contaminazione di molte specie di Mycoplasma, tra cui le cinque più comuni: Il sequenziamento dei prodotti di PCR consente di determinare la specie di appartenenza. Semplice e veloce: tutti i componenti della miscela di reazione sono aliquotati in singole provette. E sufficiente aggiungere il campione sotto forma di lisato cellulare (bollitura 5 min) o di DNA genomico. Dopo la reazione di PCR il campione è pronto per essere caricato su gel. Disponibile per 96 reazioni. Sensibile: la contaminazione viene rilevata in campioni contenenti 5x10 4 cellule oppure ng di DNA genomico. Codice Prodotto Confezione INT e-myco PCR Mycoplasma Detection Kit 96 Tubes 24

23 intron: Kit PCR, descrizione tecnica 25

24 intron: Related Products DNA/RNA extraction Codice Prodotto Descrizione Applicazione Confezione INT INT INT Viral Gene-spin Viral DNA/RNA Extraction kit G-spin Genomic DNA Extraction kit(for Blood) easy-spin Total RNA Extraction Kit (DNA free) Viral Gene-spin kit is designed for rapid isolation of DNA or RNA from a variety of sample sources including fresh or frozen plasma, serum, other cellfree body fluids and virus-infected cell/tissue. G-spin Genomic DNA Extration Kit for blood falcilitates the simple and efficient extraction of genomic DNA from large volumes of blood, including archived or poorly stored samples. easy-spin Total RNA Extraction Kit (DNA free) removes virtually all genomic DNA, it also combines the advantages of solution type products and column type ones, removing the inconveniency of alcohol PPT process in solution type products and enabling the extration of total RNA without genomic DNA. DNA/RNA DNA RNA 50 column 50 column 50 column PCR/RT-PCR pre-mix Codice Prodotto Descrizione Applicazione Confezione INT INT INT INT INT INT INT Maxime PCR PreMix (i-taq) Maxime PCR PreMix (i-startaq) Maxime PCR PreMix (i-pfu) Maxime RT-PCR PreMix PCR Starter Kit (MS-1 Type) Maxime PCR PreMix kit has not only various kinds of premix kit according to experience purpose, but also 2X mastermix solution. Maxime PCR PreMix(i-Taq) is the product in which every component is mixed : i-taq polymerase, dntp mixture, reaction buffer, etc. in one tube for 1 reaction PCR. This is the product that can get the best result with the most convenient system. Maxime PCR PreMix(i-StarTaq) is the product in which every component is mixed : i-startaq polymerase, dntp mixture, reaction buffer, etc. in one tube for 1 reaction PCR. This is the product that can get the best result with the most convenient system. Maxime PCR PreMix(i-pfu) is made with intron i-pfu polymerase. i-pfu polymerase is a thermostable DNA polymerase purified from an E.coli strain, carrying a plasmid with the cloned gene encoding Pyrococcus DNA polymerase. Maxime PCR PreMix (i-pfu) is the product that mix every component: i-pfu polymerase, dntp mixture, reaction buffer, and on-in one tube for 1 reaction PCR. Maxime RT-PCR PreMix kit contains every component for each reaction. The result is the synthesis of the first-strand cdna and PCR from total RNA or mrna template. MaximeRT-PCR PreMix uses OptiScript RT system, so accuracy and high efficience RT-reaction can do from 50fg to 500ng of template RNA, and it is developed with the best condition of first-strand cdna synthesis, so it is useful for check a low copy of DNA transcription. In addition, it block PCR from unspecific binder primer or primer-dimer by i-startaq polymerase contains hot-start PCR. PCR Starter Kit (MS-1 Type) consists of different kinds of Taq DNA Polymerases per tube, such as premium standard i-taq TM DNA Polymerase, i- StarTaq TM DNA Polymerase (hot-start), i-max TM II DNA Polymerase (long and high-fidelity), and i- StarMAX TM II DNA Polymerase (hot-start, and long and high-fidelity). This kit provides the optimal PCR condition by containing various kinds of Taq DNA Polymerase. PCR Starter Kit (MS-1 Type) is designed to resolve the difficulties encountered in the selection of Taq DNA Polymerases and set up the perfect PCR condition PCR PCR PCR RT-PCR PCR 96 tubes (20 µl) 480 tubes (20 µl) 96 tubes (20 µl) 480 tubes (20 µl) 96 tubes (20 µl) 96 tubes (20 µl) 96 tubes (20 µl) 26

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