Plasmid DNA, Gel Extraction & PCR Products Purification Kits. Code Description Size. BS363 EZ-10 Spin Column PCR Products Purification Kit 50 preps

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1 Plasmid DNA, Gel Extraction & PCR Products Purification Kits Code Description Size BS363 EZ-10 Spin Column PCR Products Purification Kit BS364 EZ-10 Spin Column PCR Products Purification Kit 100 preps BS664 EZ-10 Spin Column PCR Products Purification Kit 2 BS Well Plate PCR Products Purification Kit 2x Plates BS Well Plate PCR Products Purification Kit 5X Plates BS367 EZ-10 Spin Column DNA Cleanup Mini-preps Kit BS368 EZ-10 Spin Column DNA Cleanup Mini-preps Kit 100 preps BS668 EZ-10 Spin Column DNA Cleanup Mini-preps Kit 2 BS Well Plate DNA Cleanup Mini-preps Kit 2x Plates BS Well Plate DNA Cleanup Mini-preps Kit 5X Plates BS353 EZ-10 Spin Column DNA Gel Extraction Kit BS354 EZ-10 Spin Column DNA Gel Extraction Kit 100 preps BS654 EZ-10 Spin Column DNA Gel Extraction Kit 2 BS413 EZ-10 Spin Column Plasmid DNA Mini-preps Kit BS414 EZ-10 Spin Column Plasmid DNA Mini-preps Kit 100 preps BS614 EZ-10 Spin Column Plasmid DNA Min-preps Kit 2 BS4139 EZ-10 Spin Column Plasmid DNA Mini-preps Kit, LOW COPY PLASMIDS BS4149 EZ-10 Spin Column Plasmid DNA Mini-preps Kit, LOW COPY PLASMIDS 100 preps BS6149 EZ-10 Spin Column Plasmid DNA Min-preps Kit, LOW COPY PLASMIDS 2 BS82014 Ultra-Fast Spin Column Plasmid DNA Min-Preps Kit BS4654 EZ-500 Spin Column Plasmid DNA Maxi-preps Kit 4 preps BS466 EZ-500 Spin Column Plasmid DNA Maxi-preps Kit 20 preps BS Well Plate Plasmid DNA Mini-Preps Kit 2X Plates BS Well Plate Plasmid DNA Mini-Preps Kit 5X Plates BS92028 Ultra-Fast 96-Well Plate Plasmid DNA Mini-Preps Kit 2X Plates BS92029 Ultra-Fast 96-Well Plate Plasmid DNA Mini-Preps Kit 5X Plates PT Well Plate Single Colony Plasmid DNA Mini-Preps Kit 2X Plates PT Well Plate Single Colony Plasmid DNA Mini-Preps Kit 5X Plates BS71918 EZ-10 Spin Column Endotoxin-Free Plasmid DNA Mini-Preps Kit 50 Preps SK1249 EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit 4 Maxi-Preps SK1250 EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit 20 Maxi-Preps BS467 EZ-10 Spin Column Yeast Plasmid DNA Mini-Preps Kit 10 Preps BS468 EZ-10 Spin Column Yeast Plasmid DNA Mini-Preps Kit 50 Preps BS469 EZ-10 Spin Column Yeast Plasmid DNA Mini-Preps Kit 250 Preps Genomic DNA Isolation Kits Code Description Size BS8202 EZ-DNA Reagents 100 Preps BS8413 One-Tube Bacteria Genomic DNA Isolation Kit 100 Preps BS8414 One-Tube Bacteria Genomic DNA Isolation Kit 500 Preps BS8225 Rapid Bacteria Genomic DNA Isolation Kit 50 Preps BS423 EZ-10 Spin Column Bacterial Genomic DNA Mini-Preps Kit 50 Preps BS624 EZ-10 Spin Column Bacterial Genomic DNA Mini-Preps Kit 250 Preps SK Well Plate Bacteria Genomic DNA Mini-Preps Kit 2X Plates 1

2 SK Well Plate Bacteria Genomic DNA Mini-Preps Kit 5X Plates PT92038 EZ-500 Spin Column Bacteria Genomic DNA Maxi-Preps Kit 4 Preps BS8227 Rapid Yeast Genomic DNA Extraction Kit 50 Preps BS8257 EZ-10 Spin Column Yeast Genomic DNA Mini-Preps Kit 50 Preps BS Well Plate Yeast Genomic DNA Mini-Preps Kit 2X Plates FT71415 Rapid Fungal Genomic DNA Isolation Kit 50 Preps FT82012 EZ-10 Spin Column Fungal Genomic DNA Mini-Preps Kit 50 Preps PT92037 EZ-500 Spin Column Fungal Genomic DNA Maxi-Preps Kit 4 Preps PT71816 One-tube Plan Genomic DNA Extraction Kit 100 Preps PT71817 One-tube Plan Genomic DNA Extraction Kit 250 Preps BS425 EZ-10 Spin Column Plant Genomic DNA Mini-Preps Kit 50 Preps BS626 EZ-10 Spin Column Plant Genomic DNA Mini-Preps Kit 250 Preps PT92035 EZ-500 Spin Column Plant Genomic DNA Maxi-Preps Kit 4 Preps PT92036 EZ-500 Spin Column Plant Genomic DNA Maxi-Preps Kit 20 Preps BS Well Plate Plant Genomic DNA Mini-Preps Kit 2X Plates ST82316 EZ-10 Spin Column Soil DNA Mini-Preps Kit 50 Preps BS8401 One-Tube Tissue DNA Extraction Kit 100 Preps BS8402 One-Tube Tissue DNA Extraction Kit 500 Preps AT4780 Rapid Animal Genomic DNA Extraction Kit 10 Preps AT4781 Rapid Animal Genomic DNA Extraction Kit 50 Preps AT4782 Rapid Animal Genomic DNA Extraction Kit 250 Preps BS427 EZ-10 Spin Column Animal Genomic DNA Mini-Preps Kit 50 Preps BS628 EZ-10 Spin Column Animal Genomic DNA Mini-Preps Kit 250 Preps PT92034 EZ-500 Spin Column Animal Genomic DNA Maxi-Preps Kit 4 Preps BS Well Plate Animal Genomic DNA Mini-Preps Kit 2X Plates BS Well Plate Animal Genomic DNA Mini-Preps Kit 5X Plates BS8403 One-Tube Clinical Sample DNA Extraction Kit 100 Preps BS8404 One-Tube Clinical Sample DNA Extraction Kit 500 Preps BT4782 Rapid Blood Genomic DNA Extraction Kit 50 Preps BS483 EZ-10 Spin Column Blood Genomic DNA MiniPreps Kit 50 Preps BS684 EZ-10 Spin Column Blood Genomic DNA MiniPreps Kit 250 Preps BT Well Plate Blood Genomic DNA Mini-Prep Kit 2 Plates BT Well Plate Blood Genomic DNA Mini-Prep Kit 5 Plates VT4785 One Tube Viral DNA Isolation Buffer 50 Preps VT4786 One Tube Viral DNA Isolation Buffer 100 Preps VT81812 EZ-10 Spin Column Viral DNA Mini-Preps Kit 50 Preps VT81813 EZ-10 Spin Column Viral DNA Mini-Preps Kit 250 Preps VT Well Plate Viral DNA Mini-Preps Kit 2X Plates T71612 One-Tube Swab DNA Extraction Kit 20 Preps T71613 One-Tube Swab DNA Extraction Kit 100 Preps T71614 One-Tube Swab DNA Extraction Kit 500 Preps 2

3 BS8407 One-Tube Hair DNA Extraction Kit 50 Preps BS88503 ONE-4-ALL Genomic DNA Miniprep Kit 50 Preps BS88504 ONE-4-ALL Genomic DNA Miniprep Kit 100 Preps BS88505 ONE-4-ALL Genomic DNA Miniprep Kit 250 Preps DNA/RNA/Protein Isolation Kit Code Description Size BS88003 All-In-One DNA/RNA/Protein Miniprep Kit 50 Preps BS88203 All-In-One DNA/RNA Miniprep Kit 50 Preps RNA Isolation Kit Code Description Size BS409A One Step RNA Reagent 25 ml BS410A One Step RNA Reagent 100 ml BS1361 EZ-10 Spin Column Total RNA Mini-preps Kit 50 Preps BS8625 Rapid Bacteria RNA Isolation Kit 50 Preps BS583 EZ-10 Spin Column Total RNA Mini-Preps Super Kit 20 Preps BS584 EZ-10 Spin Column Total RNA Mini-Preps Super Kit 100 Preps BS784 EZ-10 Spin Column Total RNA Mini-Preps Super Kit 250 Preps BS Well Plate Bacterial Total RNA Mini-Preps Super Kit 2X Plates BS Well Plate Bacterial Total RNA Mini-Preps Super Kit 5X Plates BS91315 EZ-10 Spin Column RNA Cleanup & Concentration Kit 50 Preps BRC Well Plate RNA Cleanup & Concentration Kit 2x Plates FT71416 Rapid Fungal RNA Extraction Kit 50 Preps BS91915 EZ-10 Spin Column Fungal RNA Mini-Preps Kit 50 Preps PT4191 Rapid Plant RNA Isolation Kit 50 Preps BS82314 EZ-10 Spin Column Plant RNA Mini-Preps Kit 50 Preps VT4184 Rapid Viral RNA Extraction Kit 50 Preps VT82112 EZ-10 Spin Column Viral Total RNA Extraction Kit 50 Preps AT4181 Rapid Animal Total RNA Extraction Kit 50 Preps AT4182 Rapid Animal Total RNA Extraction Kit 250 Preps BS82312 EZ-10 Spin Column Animal Total RNA Mini-Preps Kit 50 Preps BS82322 EZ-10 Spin Column Animal Total RNA Mini-Preps Kit 250 Preps BT4182 Rapid Blood RNA Isolation Kit 10 Preps BT4183 Rapid Blood RNA Isolation Kit 50 Preps BT4184 Rapid Blood RNA Isolation Kit 250 Preps BS82313 EZ-10 Spin Column Blood RNA Mini-Preps Kit 50 Preps MT91928 mrna Purification Kit from Total RNA 25 Preps BS88583 EZ-10 Total RNA Miniprep Kit 50 Preps BS88253 Rnase-Free DNAse Set 50 Preps BS88133 EZ-10 DNAway RNA Miniprep Kit 50 Preps 3

4 BS88136 EZ-10 DNAway RNA Miniprep Kit 250 Preps More Kits Code Description Size 9K Gel Extraction Kit (100 preps) 100 Preps 9K Gel Extraction Kit (200 preps) 200 Preps 9K PCR Purification Kit (100 preps) 100 Preps 9K PCR Purification Kit (200 preps) 100 Preps 9K Gel and PCR Extraction System (100 preps) 100 Preps 9K Gel and PCR Extraction System (200 preps) 200 Preps 9K Plasmid Miniprep Kit (100 preps) 100 Preps 9K Plasmid Miniprep Kit (200 preps) 200 Preps 9K Genomic DNA Extraction. Blood Genomic DNA Prep Kit (400Rx) 400 Rxn 9K Genomic DNA Extraction. Bacteria Genomic DNA Prep Kit (400Rx) 400 Rxn 9K Genomic DNA Extraction. Plant Genomic DNA Prep Kit (400Rx) 400 Rxn 9K Animal Tissue & Fungi Genomic DNA Extraction Prep Kit (400Rx) 400 Rxn 9K Yeast Genomic DNA Extraction Prep Kit (400Rx) 400 Rxn 9K Genomic DNA Extraction. Blood Genomic DNA Prep Kit (3300Rx) 400 Rxn 9K Genomic DNA Extraction. Bacteria Genomic DNA Prep Kit (3300Rx) 3300 Rxn 9K Genomic DNA Extraction. Plant Genomic DNA Prep Kit (3300Rx) 3300 Rxn 9K Animal Tissue & Fungi Genomic DNA Extraction Prep Kit (3300Rx) 3300 Rxn 9K Yeast Genomic DNA Extraction Prep Kit (3300Rx) 3300 Rxn 9K Plasmid DNA Maxiprep (10 preps) 10 Preps 9K Plasmid DNA Maxiprep (30 preps) 30 Preps 9K Plasmid DNA Midiprep (25 preps) 25 preps Columns & 96 Well Plates Code Description Size SD5005 EZ-10 Column & collection tube (blue tube, clear ring, clear collection) 100 SD5005- EMPTY EZ-10 Empty Column without filter, without collection tube & O-ring 1000 SD5008 EZ-10 RNA Column & collection tube(clear tube, clear ring, clear collection) 100 SD5003 EZ-200 Column & collection tube (set) 12 SD5004 EZ-500 Column & collection tube (set) 12 SD well filter plate (960ul each well) 12 SD5006- EMPTY 96 well filter plate (no filter) 12 SD well DNA plate with membrane (960ul each well) 12 SD well RNA plate with membrane (960ul each well) 12 SD well sample storage plate (200ul each well), 10/PK 1PK SD Well Vacuum System 1 SD5012 Medi preps or Maxi preps Column Plug 1 BR480-N 48 WELL 4ML U-SHAPED DEEP-WELL PLATES, 10/BAG 1PK BR581-96N 96 WELL 2ML DEEP PLATE, NATURAL,EDGE FILLED, 10PLATES/BAG 1PK 4

5 Bio Basic Inc. EZ-Kits Overview Bio Basic Inc. supplies a remarkable variety of molecular biology kits, including Plasmid Miniprep, Gel Extraction, PCR Product Purification, and a complete line of Genomic DNA and RNA isolation kits offered in both spin column and 96-well plate formats! Although the detailed protocol for each kit varies, the general three-step principle remains the same: (1) BIND, (2) WASH, (3) ELUTE. Bio Basic s EZ-Membrane allows for fast and efficient purification, regardless of sample type, thereby providing you with high yields of purified product with minimal handling and effort. Spin Column Format EZ-Protocol 96-Well Plate Format (1) BIND Starting material is bound to Bio Basic s EZ-Membrane in spin column or plate format. (2) WASH The bound material is washed to remove impurities, buffers, and other unwanted materials. (3) ELUTE The purified product (DNA/RNA/Protein) is eluted from the membrane and collected for downstream application! 5

6 Plasmid DNA, Gel Extraction & PCR Products Purification Kits EZ-10 Spin Column PCR Products Purification Kit This kit utilizes membrane technology which selectively adsorbs up to 20ug of PCR products or DNA fragments in the presence of a specialized binding buffer. Primers (<40-mer), nucleotides, enzymes, mineral oil and all other impurities are unable to bind and are eliminated from the column. PCR products or DNA fragments are eluted readily in elution buffer. PCR products and DNA fragments can be used readily in all downstream applications including digestion, cloning and DNA sequencing. The kit can also be used for concentrating DNA fragments as well as removal of impurities such as salts and proteins. 1. Fast. Using a rapid spin column format, the entire procedure take less than 10 minutes. 2. High Recovery. For fragments of 100 bp-10 kb, >85% of DNA will be recovered. 3. High Quality of DNA. Purified PCR products and DNA fragments can be used readily in all downstream applications including digestion, cloning and DNA sequencing. 4. Non-Toxic. No phenol/chloroform is required. 5. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. BS Preps BS Preps BS Preps Buffer B3 16ml 32ml 80ml Wash Solution 12ml 24ml 60ml Elution Buffer 5ml 10ml 25ml EZ-10 Column ml Collection Tube Gel electrophoresis of PCR products purified using EZ-10 Spin Column PCR Products Purification Kit. 96-Well Plate PCR Products Purification Kit This kit provides a simple and efficient high throughput technique for the cleanup of PCR products and DNA fragments from gel extraction and enzymatic reaction solutions. PCR products or enzymatic reactions are mixed with a specialized binding buffer and pass through 96 columns of the plate. Impurities such as enzymes, salts, nucleotides and primers (< 40-mer) are washed away. Pure DNA is eluted with a small volume of low-salt elution buffer or water, and is ready for downstream applications including DNA sequencing and cloning. 1. Fast. Using a rapid spin column and 96-well high throughput format, the entire procedure take less than 30 minutes. 2. High Recovery. For fragments of 100 bp-10 kb, >85% of DNA will be recovered. 3. High Quality of DNA. Purified PCR products and DNA fragments can be used readily in all downstream applications including digestion, cloning and DNA sequencing. 4. Non-Toxic. No phenol/chloroform is required. 5. Direct manufacturer with most competitive price advantage. BS x 96 well Plates Buffer B3 60ml 2X75ml Wash Solution 2X35ml 4X48ml Elution Buffer 12ml 30ml EZ Well Plate 2 5 Deep Well Collection Plate Storage Plate 2 5 Sealing film 8 20 Complete 1 BS365 5 x 96 well Plates Reproducible yields of PCR products purified using the 96-Well Spin Column PCR Products Purification Kit. Lane 1: DNA marker. Lanes 2-5: Crude PCR products. Lanes 6 to 25: Purified PCR products. Recoveries and yields are approximately 80%. 6

7 EZ-10 Spin Column DNA Cleanup Mini-Preps Kit This kit provides a simple and efficient method for the purification of DNA fragments from various enzymatic reactions, including cdna synthesis, restriction digestions, and PCR, among others. It is also well-suited for desalting a solution of DNA, as well as the removal of residual organic solvents or unincorporated nucleotides or primers (<40-mer) from reaction mixtures. The kit utilizes spin column technology which selectively adsorbs up to 20ug of DNA in the presence of specialized binding buffers. All impurities that do not bind are eliminated from the column. DNA fragments can be eluted readily with elution buffer. Purified DNA fragments and PCR products can be used for most downstream applications including sequencing and cloning. 1. Fast. Using a rapid spin column format, the entire procedure takes less than 10 minutes. 2. High Recovery. For fragments of 100 bp-10 kb, >85% of DNA will be recovered. 3. High Quality of DNA. Purified PCR products and DNA fragments can be used readily in all downstream applications, including digestion, cloning and sequencing. 4. Non-Toxic. No phenol/chloroform is required. 5. Direct manufacturer with most competitive price advantage. BS Preps BS Preps BS Preps Clean Up Solution 20ml 40ml 4X25ml Wash Solution 20ml 2X20ml 2X40ml Elution Buffer 5ml 10ml 25ml EZ-10 Column ml Collection Tube Gel electrophoresis of PCR products purified using EZ-10 Spin Column DNA Cleanup Mini-preps Kit. 96-Well Plate DNA Cleanup Mini-Preps Kit This kit provides a simple and efficient high throughput method for the cleanup of PCR products and other DNA fragments from gel extraction solutions and enzymatic reactions. The kit utilizes a simple bind-wash-elute procedure. PCR products or other enzymatic reactions are mixed with a specialized binding buffer and pass through 96 columns of the plate. Whereas DNA binds to the column, impurities such as enzymes, salts, nucleotides and primers (< 40-mer) are washed away. Pure DNA can be eluted in a small volume of the low-salt elution buffer or water, ready for use in all downstream applications including DNA sequencing and cloning. Phenol/chloroform extraction and ethanol precipitation are not required. Recovering yield is typically 60-80%. Up to 20ug of purified DNA fragments in each well can be obtained. It is suitable to recover 100 bp-40 kb DNA fragments. 1. Fast. Using a rapid spin column and 96-well high throughput format, the entire procedure takes less than 30 minutes. 2. High recovery. For fragments of 100 bp-10 kb, >85% of DNA will be recovered. 3. High quality of DNA. Purified PCR products and DNA fragments can be used readily in all downstream applications including digestion, cloning and DNA sequencing. 4. Non-Toxic. No phenol/chloroform is required. 5. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. BS x 96 well Plates Clean Up Solution 60ml 2X75ml Wash Solution 2X35ml 4X48ml Elution Buffer 12ml 30ml EZ Well Plate 2 5 Deep Well Plate Well Storage Plate 2 5 Sealing film BS369 5 x 96 well Plates Reproducible yields of high quality PCR products purified using the EZ well Plate DNA Cleanup Mini-Prep Kit. 7

8 EZ-10 Spin Column DNA Gel Extraction Kit This kit provides a simple and rapid method for the purification of DNA fragments from agarose gel. It is also well-suited for desalting, as well as the removal of residual organic solvents or unincorporated nucleotides or primers (<40-mer) from reaction mixtures containing DNA. The kit utilizes a spin column technology which selectively adsorbs up to 20ug of DNA fragments in the presence of specialized binding buffers. Nucleotides, enzymes, mineral oil and other impurities do not bind to the column and are eliminated. DNA fragments can be eluted readily in elution buffer and used for DNA sequencing, restriction digestion, and other downstream applications. Recovering yield ranges from 60 to 80%. No phenol/chloroform extraction or ethanol precipitation is required. 1. Fast. Using a rapid spin column format, the entire procedure takes less than 15 minutes. 2. High Recovery. Yield ranges from 60 to 80%. 3. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. BS Preps BS Preps BS Preps Binding Buffer II 50ml 2X50ml 5X50ml Wash Solution 20ml 2X20ml 2x40ml Elution Buffer 5ml 10ml 25ml EZ-10 Column ml Collection tube DNA fragments purified from an agarose gel using the Gel Extraction Kit. EZ-10 Spin Column Plasmid Mini-Preps Kit This kit provides a simple and efficient method for the purification of plasmid DNA from bacterial culture. Plasmid DNA is selectively adsorbed by the EZ-10 column, while impurities such as proteins, salts and nucleotides are washed away. In order to maximize the recovery yield of plasmid DNA, a color indicator VisualLyse - is added to the buffer, which prevents insufficient or over-lysis during the lysis and neutralization step. Note: This kit is designed for the preparation of up to 20ug of plasmid DNA. If larger amounts are required, plasmid Maxi preps are recommended. For low copy plasmid, BS4139, BS4149 and BS6149 are recommended. Solutions are doubled in low copy plasmid kits. 1. Fast. Using a rapid spin column format, the entire procedure takes only 12 minutes. 2. High Purity of DNA. OD260/OD280 ratio of purified DNA is , and can be used directly in most downstream applications such as sequencing, PCR, transformation, restriction digestion and ligation. 3. Non-toxic. No phenol/chloroform extraction or ethanol precipitation is required. 4. Highest yield is guaranteed by using VisulLyse reagent. 5. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature.. BS Preps BS Preps BS Preps Solution I 6ml 12ml 30ml Solution II* 12ml 24ml 60ml Solution III 25ml 50ml 125ml Wash Solution** 20ml 2X20ml 2X40ml Elution Buffer 5ml 10ml 25ml RNase A (10mg/ml) 120ul 240ul 600ul VisualLyse 60ul 120ul 300ul EZ-10 Column ml Collection Tube *Solution II may form a precipitate upon storage. If necessary, dissolve the precipitate by warming at 37 0 C. ** Before use for the first time, add four volumes of 96%-100% ethanol to Wash Solution (v/v). Gel electrophoresis of plasmid DNA purified using the EZ-10 Plasmid DNA Mini-preps Kit. 8

9 Ultra-Fast Spin Column Plasmid DNA Mini-Preps Kit This kit facilitates single-step plasmid DNA purification from bacterial cultures. Using a one buffer system as opposed to the traditional three, the kit releases plasmid DNA rapidly from your sample. Although the yield of the recovered plasmid DNA is slightly lower than that from regular kits, it is much faster and simpler to use. Bacterial cultures are lysed and the lysates are directly loaded on to the spin column. Plasmid DNA is selectively bound onto the EZ-10 column and other impurities are washed away. Pure plasmid DNA is eluted in low-salt buffer or water, and can be readily used for many downstream applications. This kit is suitable for the preparation of up to 8ug of pure plasmid DNA. 1. Fast and Simple: One step lysis of cell cultures. The entire procedure takes less than 10 minutes. 2. High Purity of DNA: OD260/OD280 of plasmid DNA purified using this kit is >1.8. Purified plasmid DNA can be used for many downstream applications, including DNA sequencing, cloning, PCR, transformation and restriction digestions. 3. Non-toxic: phenol/chloroform extraction and ethanol precipitation are not required. 4. Direct manufacturer with most competitive price advantage. Transportation: At ambient room temperature. Lysis Buffer UF Buffer DW1 Universal Wash Solution Elution Buffer BS Preps 50ml 24ml 12ml 5ml EZ-10 Column ml Collection Tube 50 Plasmid DNA purified using the Ultra-Fast EZ-10 Spin Column Plasmid DNA Mini-preps Kit. EZ-500 Spin Column Plasmid DNA Maxi-Preps Kit This kit provides a simple and efficient method for purifying large amounts of plasmid DNA. Up to 500ug of plasmid DNA is selectively adsorbed on the 25ml EZ-500 column. Impurities, including proteins, salts and nucleotides, are eliminated from the column and washed away. Plasmid DNA can be eluted in 3ml of Elution buffer. No phenol/chloroform or ethanol precipitations are required. DNA purified using the kit can be used in many downstream applications including transformation, DNA sequencing, PCR, and Southern-blotting. In order to obtain a maximal recovery yield, a color indicator - VisualLyse - is added to the buffer which prevents insufficient or over-lysis during the lysis and neutralization step. Specification: Sampling Yield Handling Time 100~250 ml of bacteria culture for high copy plasmids up to 500 mg ~2 hours 200~400 ml of bacteria culture for low copy plasmids for high-copy plasmid Transportation: At ambient temperature. BS4654 BS466 4 Preps 20 Preps MAXI 1 Resuspension Solution 55 ml 2X110 ml MAXI 2 Cell Lysis Solution 55 ml 2X110 ml MAXI 3 Neutralization Solution 55 ml 2X110 ml MAXI 4 Equilibration Solution 54 ml 2X135 ml MAXI 5 Washing Solution 4X55 ml 4X275 ml MAXI 6 Elution Solution 54 ml 2X135 ml RNase A (50 mg / ml) 88 ml 440 ml MAXI Column 4 20 Collection Tube Solutions provided in this kit contain irritants, wear gloves and lab coat when handling. 2. Briefly spin RNase A tube to remove drops from the inside of the lid. Transfer tube content into MAXI 1 Resuspension Solution bottle. Add 250µL of MAXI 1 Resuspension Solution into RNase A tube, rinse tube inside and transfer back into MAXI 1 Resuspension Solution bottle. Store at 4 C. 3. Check MAXI 2 Cell Lysis Solution before use. Warm MAXI 2 Cell Lysis Solution at 37 C if any precipitation formed. Prevent vigorous shaking of the MAXI 2 Cell Lysis Solution. 4. To avoid acidification of MAXI 2 Cell Lysis Solution from CO 2 in the air, close the bottle immediately after use. 9

10 96-Well Plate Plasmid DNA Mini-Preps Kit This kit provides a rapid, high-throughput method for 96-well plasmid DNA purification. Plasmid DNA is selectively adsorbed on silica membranes in the 96-well plate. Proteins, salts, nucleotides and other contaminants are subsequently eliminated from the column. Purified plasmid DNA can then be eluted in a small volume of elution buffer. In order to obtain maximal yield, a color indicator VisualLyse - is added to the buffer which prevents insufficient or over-lysis during the lysis and neutralization step. Purified DNA can be used directly in a variety of applications including sequencing, PCR and Southern-blotting. Note: This kit is used for the preparation of a maximum of 20ug of pure plasmid DNA per well. 1. Fast. Using the rapid spin column and high-throughput format, the entire procedure takes only 30 minutes. 2. High DNA Yield. Up to 20μg DNA in each well can be obtained. 3. High Purity of DNA. OD260/OD280 ratio of purified DNA is typically Non-toxic. No phenol/chloroform extraction or ethanol precipitation is required. 5. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. BS plates BS415 5 plates Solution I 25ml 60ml Solution II* 50ml 120ml Solution III 90ml 210ml Wash Solution** 2X35ml 4X48ml Elution Buffer 15ml 30ml RNase A (10mg/ml) 0.5ml 1.2ml VisualLyse 240ul 600ul EZ Well Plate 2 5 Deep Well Collection Plate Storage Plate 2 5 Reproducible yields of plasmid DNA purified using the 96- Well Plate Plasmid DNA Mini-preps Kit. Sealing film *Solution II may form a precipitate upon storage. If necessary, dissolve the precipitate by warming at 37 C. ** Before use for the first time, add four volumes of 96%-100% ethanol to Wash Solution. Ultra-Fast 96-Well Plate Plasmid DNA Mini-Preps Kit This kit is designed for one-step plasmid DNA purification from bacterial culture using a rapid and high throughput spin column format. Using a one buffer solution and replacing the traditional three, the kit releases plasmid DNA rapidly from bacterial culture. Although the yield of the recovered plasmid DNA is slightly lower than that from regular 96-Well Plate Kits, it is simpler and faster. Bacterial cultures are lysed and the lysates are directly loaded to each column of the 96-Well plate. Plasmid DNA is selectively bound onto the 96-well plate and other impurities are washed away. Pure plasmid DNA is eluted in Elution buffer or water, and can be readily used for most downstream applications. This kit is used for preparation of up to 6-8ug of pure plasmid DNA. 1. Fast & Simple. Using one step lysis spin column and 96-well high throughput format, the entire procedure takes only 20 minutes. 2. High Purity of DNA. OD260/OD280 of plasmid DNA purified by this kit is >1.8. Purified plasmid DNA can be used for many downstream applications such as DNA sequencing, cloning, PCR, transformation and restriction digestions. 3. Non-toxic. No phenol/chloroform extraction and ethanol precipitation are required. Transportation: At ambient temperature. BS plates BS plates Lysis-Buffer-UF 200ml 2X250ml Buffer DW1 100ml 2X120ml Universal Wash Solution II 48ml 2X48ml Elution Buffer 20ml 50ml EZ Well Plate 2 5 Deep Well Collection Plate Storage Plate 2 5 Sealing film

11 96-Well Plate Single Colony Plasmid DNA Mini-Preps Kit This kit provides a simple, efficient and high throughput method for plasmid DNA purification. Plasmid DNA and DNA fragments are selectively adsorbed onto the EZ-10 columns of the 96-well plate. Other impurities such as proteins, salts and nucleotides are washed away. Plasmid DNA can then be eluted in a small volume of elution buffer. Purified plasmid DNA can be used for many downstream applications, including PCR, DNA sequencing, enzyme digestion and transformation. Note: the diameter of each colony should be >2 mm. 1. Fast. Using a rapid spin column and 96-well high throughput format, the entire procedure takes 20 minutes. 2. Direct amplification from colonies, or glycerol stock solutions, no overnight culture is required. 3. Suitable for fast and large number screening. 4. Compatible with all downstream applications including PCR, restriction analysis and DNA sequencing. 5. Economic. Transportation: at ambient room temperature. PT plates PT plates Lysis-Buffer-UF 200ml 2X250ml Buffer DW1 100ml 2X120ml Universal Wash Solution II 48ml 2X48ml Elution Buffer 20ml 50ml EZ Well Plate 2 5 Deep Well Collection Plate Storage Plate 2 5 Sealing film puc plasmid DNA purified using the 96-well single colony plasmid DNA MiniPrep Kit. EZ-10 Spin Column Endotoxin-Free Plasmid DNA Mini-Preps Kit This kit provides a simple and efficient method for high quality endotoxin-free plasmid DNA purification from bacterial culture. Greater than 90% of the endotoxin can be removed using our Endotoxin-Be-Gone reagent. If large scale preparation is needed, EZ-500 Column can be used (SK1249 & SK1250). Purified endotoxin-free plasmid DNA is suitable for transfection and other downstream applications. 1. Fast and convenient. Endotoxin is removed at the bacteria stage and the remaining steps are regular plasmid DNA extraction steps. 2. Efficient. A single treatment removes 90% of the endotoxin. Repeat procedure for greater efficiency. Purified plasmid DNA can be used for many downstream applications including transfection. 3. Economic. Solution I Solution II Solution III Wash Solution Elution Buffer Liquid Endotoxin Eliminator EZ-10 Column 50 RNase A Solution (10mg/ml) 2.0ml Collection Tube 50 BS Preps 6ml 12ml 25ml 20ml 5ml 5ml 120μl 11

12 EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit This kit provides a large-scale, simple and efficient method for high quality endotoxin-free plasmid DNA purification from bacterial culture. Up to 500ug of endotoxin-free plasmid DNA may be obtained per maxi prep. Greater than 90% of the endotoxin can be removed using bacterial endotoxin erasol at bacteria stage. Purified endotoxin-free plasmid DNA can be used for transfection and other downstream applications. 1. Fast. Using a maxi-prep spin-column format, the entire procedure takes only 40 minutes. 2. High Quality of DNA. >90-99% of endotoxin can be removed. 3. Economic. Store: at room temperature. The kit is stable for 1 year. Buffer Solution I with RNase A should be kept at 2-8 C, valid for 6 months. SK Maxi-Preps SK Maxi-Preps Solution I 100ml 2 X 240 ml Solution II 200ml 4 x 240ml Solution III 2X150ml 6 x 250ml Wash Solution 10ml 48ml Elution Buffer 8ml 40ml Liquid Endotoxin Eliminator 8ml 40ml RNase A Solution (10mg/ml) 2ml 10ml EZ-500 Column ml Collection Tube EZ-10 Spin Column Yeast Plasmid DNA Mini-Preps Kit The kit provides a simple and efficient Spin Column approach for Mini plasmid DNA purification from yeast. Plasmid DNA of lysed yeast cells is selectively bound onto the column. Other impurities such as proteins and salts do not bind to the column and are eliminated in the flow through. Purified plasmid DNA can be used for PCR, transformation, restriction digestion and many other downstream applications.1ug of plasmid DNA can be purified from 5ml yeast cells. 1. Fast. Using a rapid spin-column format, the entire procedure takes approximately 2 hours. 2. High Quality of DNA. OD260/OD280 of purified DNA is generally > Compatible with most downstream applications such as PCR, restriction digestion and hybridization. 4. Non-Toxic. Phenol/chloroform extractions or ethanol precipitation is not required. 5. Direct manufacturer with most competitive price advantage. Transportation: at ambient temperature BS Preps BS Preps BS Preps Buffer P1 3ml 15ml 75ml Buffer P2 3ml 15ml 75ml Buffer P3 4ml 20ml 100ml Buffer DW1 5ml 25ml 125ml Universal Wash Buffer 3ml 12ml 60ml Elution Buffer 1ml 5ml 25ml RNase A Solution (10mg/ml) 25ul 120ul 600ul Snailase 60mg 300mg 1500mg Snailase Storage Buffer 0.5ml 2.5ml 12.5ml Snailase Reaction Buffer 7ml 35ml 175ml EZ-10 Spin Column ml Collection Tube

13 Genomic DNA Purification & Extraction Kits EZ-DNA Reagent EZ-DNA Reagent is a ready-to-use reagent for the isolation of genomic DNA from various samples, including animal tissue, cultured cells, and bacteria. The method is based on the use of an optimized guanidine-detergent solution, which lysis the tissue quickly and selective precipitates DNA from a cell lysate. During the isolation step, tissue or cells are lysed in EZ-DNA Reagent and the genomic DNA is precipitated from the lysate with ethanol. Following an ethanol wash, purified DNA is dissolved in water, TE buffer or 8mM NaOH. The protocol allows for fast isolation of genomic DNA from a large number of samples of small or large volume. The whole procedure takes about minutes with DNA recovery of 70-90%. There is no need for overnight digestion, phenol extraction. Isolated DNA can be used directly for most applications such as Southern analysis, molecular cloning and PCR. 1. Fast. The entire procedure takes only minutes. 2. Simple. One-Tube procedure, no phenol/chloroform extraction is required. 3. Versatile. Suitable for wide range of samples including animal tissue, cultured cells, and bacteria. 4. Easy to scale up. 5. Economic. Transportation: t ambient temperature. BS preps EZ-DNA Reagent 100ml DNA extraction from various species using EZ-DNA Reagent. M. DNA marker. Lane 1-3: Genomic DNA from Bacteria. Lane 4-6: Genomic DNA from Rat. Lane 7-9: Genomic DNA from Yeast. One-Tube Bacteria Genomic DNA Isolation Kit The kit is designed for rapid small-scale extraction of high quality genomic DNA from a variety of Gram-negative and Gram-positive bacteria. Purified DNA can be used for most downstream applications, including PCR, restriction digestion, and hybridization. 1. Fast. Te entire procedure takes less than 10 minutes. 2. Simple. No phenol/chloroform extraction or ethanol precipitation is required. 3. Economic. Transportation: At ambient temperature. BS Preps BS Preps Lysis-Buffer-B 10ml 50ml Universal Buffer NST 10ml 50ml 1 DNA isolated from various bacteria. M: DNA marker. Lane 1-3: Escherichia coli (Gram-Negative). Lane 4-6: Staphyloccocus aureus (Gram-Positive). Rapid Bacteria Genomic DNA Isolation Kit The kit is designed for rapid small-scale extraction of high quality genomic DNA from a variety of Gram-negative or Gram-positive bacteria. Purified DNA can be used for many downstream applications such as PCR, restriction digestion, and hybridization. 1. Fast. It takes less than 30 minutes. 2. Simple. 3. Economic. Transportation: Room temperature Universal Digestion Buffer Buffer PB BS Preps 25ml 12ml 13

14 TE Buffer 10ml EZ-10 Spin Column Bacterial Genomic DNA Mini-Preps Kit This kit provides a simple and convenient silica membrane-based technique to isolate high quality DNA from both Gram-negative and Grampositive bacteria. Total hands-on preparation time is less than 30 minutes. Bacteria samples are lysed in an optimized buffer. Released DNA binds specifically to the column while contaminants are removed in the flowthrough. PCR inhibitors such as divalent cations and proteins are completely removed in two efficient wash steps. Pure DNA is then eluted in either water or a low-salt buffer provided with the kit. The kit is also suitable for isolation of bacterial genomic DNA from colonies on a dish. Purified genomic DNA is kb in length. Purified DNA is suitable for downstream applications such as restriction endonuclease digestions, PCR, and other applications. 1. Fast. Using a rapid spin-column format, the entire procedure takes approx 20 minutes. 2. High yield. 5-20ug of bacterial genomic DNA can be obtained from 1ml ( cells) of overnight culture. 3. High quality of DNA. OD260/280 ratio of purified DNA is generally > Non-Toxic. No phenol extraction or ethanol precipitation is required. 5. Economic Transportation: At ambient temperature. BS Preps BS Preps Digestion Buffer 20ml 100ml Wash Solution 12ml 2X30ml Elution Buffer 5ml 25ml Proteinase K 2mg 10mg EZ-10 Spin Column ml Collection Tube DNA isolated from various bacteria. M: DNA Marker Lane 1-3: Escherichia coli (Gram Negative) Lane 4-6: Staphyloccocus aureus (Gram Positive) 96-Well Plate Bacterial Genomic DNA Mini-Preps Kit The kit provides a simple and convenient high throughput approach to isolate high quality DNA from both Gram-negative and Gram-positive bacteria. DNA in a cell lysate is selectively adsorbed on to the EZ-10 column while impurities such as proteins, salts and nucleotides are washed away. No phenol/chloroform extraction or ethanol precipitation is required. Purified genomic DNA can be up to 50 kb in length. Genomic DNA purified can be used for PCR, and other downstream applications. 1. Suitable for Gram-positive and Gram-negative bacteria. 2. Fast. Using a high throughput 96-well plate format. 3. High purity of DNA. A260/280 ratio of purified DNA is generally > Compatible with downstream applications. Transportation: At ambient temperature. SK Plates SK Plates Digestion Buffer 160ml 2X200ml TE (ph=8.0) 60ml 150ml Wash Solution 50ml 3X48ml Elution Buffer 12ml 30ml Proteinase K 8mg 20mg 96-Well Plate 2 5 Deep Well Collection Plate Well Storage Plate 2 5 Sealing film E. coli genomic DNA purified using the 96-well plate mini-preps kit. Shown on 0.8% agarose with DNA ladders. 14

15 EZ-500 Spin Column Bacteria Genomic DNA Maxi-Preps Kit This kit provides a simple and convenient technique for large-scale isolation of high quality DNA from both Gram-negative and Gram-positive bacteria. Up to 500ug of DNA is selectively bound to EZ-500 spin column. Other impurities present in the lysate, including proteins and salts, do not bind on the column and are eliminated in the flow through. No phenol extraction or ethanol precipitation is required. The kit is also suitable for the isolation of bacterial genomic DNA from colonies grown on a dish. Purified genomic DNA can be up to 50 kb in length. Purified DNA is suitable for downstream applications such as restriction endonuclease digestions, PCR, and other applications. For isolation of DNA from Gram positive bacteria, bacteria should be pre-treated with a buffer containing lysozyme. 1. Suitable for both Gram-positive and Gram-negative bacteria. 2. High purity of DNA. Purified DNA has an A260/280 ratio of between 1.6 and Compatible with many downstream applications. 4. Direct manufacturer with most competitive price advantage. PT Preps Universal Digestion Buffer 40ml Universal Buffer BD 50ml Universal PW Solution 18ml Universal Wash Solution 7.5ml CE Buffer ( ph 9.0) 15ml Proteinase K (10mg/ml) 5ml Maxi Columns 4 50ml Collection Tube M E.coli genomic DNA purified using the EZ-500 Spin Column Bacteria DNA Maxi-Preps Kit. Shown on a 0.8% agarose gel. M: DNA Marker 1-7: genomic DNA from E.coli Rapid Yeast Genomic DNA Extraction Kit This kit is designed for rapid, small-scale extraction of high quality genomic DNA from yeast. Yeast cell walls are digested by Snailase (or lyticase, zymolyase) and the membrane is lysed in the presence of proprietary buffers. DNA is then precipitated and washed with alcohol. Purified DNA can be used for many downstream applications, including PCR, restriction digestion, and hybridization. 1. Fast. The entire procedure takes less than 40 minutes. 2. Easy to use. 3. Economic. BS Preps Universal Digestion Buffer Buffer PY TE Buffer Snailase Reaction Buffer Snailase Storage Buffer Snailase 25ml 12ml 10ml 75ml 5ml 600mg Genomic DNA isolated from Saccharomyces cerevisiae Lane 1-3: Genomic DNA from Saccharomyces cerevisiae. 15

16 EZ-10 Spin Column Yeast Genomic DNA Mini-Preps Kit The kit provides a simple and convenient method for the isolation of high quality yeast DNA. DNA present in cell lysate is selectively bound to the spin column while other impurities such as proteins and salts do not bind and are eliminated in the flow through. Purified genomic DNA is kb in length, and is suitable for many downstream applications, including restriction endonuclease digestion, PCR, and cloning. 1. Fast. Using a rapid spin-column format, the entire procedure takes approx 30 minutes. 2. High Quality of DNA: OD260/OD280 of purified DNA is generally Non-Toxic. No phenol extraction or ethanol precipitation is required. 4. Economic. Transportation: At ambient temperature including Proteinase K and Snailase. BS Preps Universal Digestion Buffer 12ml Universal Buffer PY 6ml Universal Buffer BD 12ml Universal PW Solution 18ml Universal Wash Solution 7.5ml CE Buffer ( ph 9.0) 15ml Proteinase K 1.2ml Snailase Reaction Buffer 75ml Snailase Storage Buffer 5ml Snailase 600mg EZ-10 Spin Column ml Collection Tube 50 Genomic DNA isolated from Saccharomycetaceae 96-Well Plate Yeast Genomic DNA Mini-Preps Kit This kit provides a simple and convenient, high-throughput approach to isolate high quality DNA from yeast. DNA present in cell lysate is selectively adsorbed onto the EZ-10 column, while impurities such as proteins, salts and nucleotides are washed away. Purified genomic DNA can be up to 50 kb in length, and can be used for a wide array of downstream applications, including PCR and restriction endonuclease digestions. 1. Fast. using the rapid spin-column and high throughput format, the entire procedure takes only 2 hours. 2. High quality of DNA. OD260/280 of purified DNA is generally Non-toxic. No phenol extraction or ethanol precipitation is required. 4. Economic. Transportation: at ambient temperature (including Proteinase K and Snailase). BS Plates Universal Buffer Digestion Universal Buffer PY Universal Buffer BD Universal PW Solution Universal Wash Solution CE Buffer (ph 9.0) Proteinase K Snailase Reaction Buffer Snailase Storage Buffer Snailase 96-Well EZ Plate 2 96-Well Collection Plate 6 96-Well Storage Plate 2 Sealing film 8 48ml 24ml 48ml 72ml 30ml 15ml 4.8ml 300ml 20ml 2.4 g Saccharomyces cerevisiae genomic DNA purified using the 96-well plate Yeast Genomic DNA Mini-Prep Extraction Kit. 16

17 Rapid Fungal Genomic DNA Isolation Kit This kit provides a simple and efficient method for the isolation of total DNA (genomic and mitochondrial) from fungal species. The size of genomic DNA purified by this procedure is around kb. Purified DNA can be readily used in a number of downstream applications including RT-PCR, SNP analysis, and restriction digestion. 1. Fast. the entire procedure takes less than 40 minutes. 2. Easy. 3. Economic. Universal Digestion Buffer Universal Buffer PF TE Buffer FT ml 12ml 10ml DNA isolated from various fungal species M: Marker Lane 1-2: Penicillium spp. Lane 3-4: Pleurotus ostreatus. EZ-10 Spin Column Fungal Genomic DNA Mini-Preps Kit This kit provides a simple and convenient method for the isolation of high quality DNA from various fungal species. DNA present in a cell lysate is selectively bound to the spin column, while other impurities such as proteins, salts, and nucelotides do not bind and are eliminated in the flow through. No phenol extraction or ethanol precipitation is required. Purified genomic DNA is kb in length, and is suitable for downstream applications, including restriction digestions, cloning, and PCR. 1. Fast and Simple. Using a spin-column format, the entire procedure takes only 30 minutes. 2. High quality of Fungal DNA. 3. Economic. FT82012 Universal Digestion Buffer Universal Buffer PF Universal Buffer BD Universal PW Solution Universal Wash Solution TE Buffer (ph 8.0) Proteinase K 12ml 6ml 12ml 18ml 7.5ml 10ml EZ-10 Spin Column 50 2-ml Collection Tube ml Price $ Genomic DNA isolated from various fungi Lane 1-3: Penicillium spp. genomic DNA Lane 4-6: Pleurotus ostreatus. DNA EZ-500 Spin Column Fungal Genomic DNA Maxi-Preps Kit This kit provides a simple and efficient method for the isolation of genomic DNA from a variety of fungal species. DNA present in a cell lysate is selectively adsorbed in the column while other impurities are eliminated. Average DNA yield is ~10-100ug per preparation. Average size of the genomic DNA purified by this procedure is around kb. No phenol/chloroform extraction, ethanol precipitation, or liquid nitrogen homogenization is required in this protocol. Purified fungal genomic DNA can be used for PCR, SNP, RAPD, RFLP, AFLP, Southern blotting, transformation and other applications. 1. Fast. 2. High Quality of DNA. OD260/280 ratio of purified fungal genomic DNA is generally > Suitable for various filamentous fungal species including S. cerevisiae, S. pombe, Pichia pastoris, Candida albicans, Aspergillus niger, Collectotrichum musae, and Fusarium oxysporum. PT preps Universal Digestion Buffer 50ml Universal Buffer PF 25ml Universal Buffer BD 50ml Universal PW Solution 18ml Universal Wash Solution 7.5ml TE Buffer (ph 8.0) 10ml Proteinase K 5ml EZ-500 Spin Columns 4 Purified fungal genomic DNA run on a 0.7% agarose gel.

18 50-ml Collection Tube 4 Rapid Plant Genomic DNA Isolation Kit This kit is designed for the rapid isolation of genomic DNA from plant leaves and seeds. Samples may be fresh, frozen, or dried. The whole procedure takes approximately 15 minutes and the kit is suitable for screening and high throughput extraction. No phenol/chloroform extraction or ethanol precipitation is required. Purified Genomic DNA can be used for RT-PCR, cloning and other downstream applications. 1. Fast. The entire procedure takes less than 30 minutes. 2. Easy. 3. Direct manufacturer with most competitive price advantage. PT preps PT preps Buffer PCL 50ml 250ml Buffer PP 24ml 120ml TE Buffer 20ml 100ml 1 High molecular genomic DNA isolated from various plant species using the Rapid Plant DNA Kit. Lane 1-2: Genomic DNA from Nicotiana tabacum Lane 3-4: Genomic DNA from Arabidopsis thaliana Lane 5-6: Genomic DNA from Oryza sativa M: DNA marker. EZ-10 Spin Column Plant DNA Mini-Preps Kit This kit provides a simple and efficient method for the isolation of plant genomic DNA. Samples may be fresh, frozen, or dried. DNA in plant tissues & cells is selectively adsorbed on the spin column, while other impurities such as proteins, salts, and lipids do not bind to the column and are washed away. Average size of genomic DNA purified by this procedure is ~30-50 kb. Purified genomic DNA can be used directly in PCR, Southern Blotting, RAPD, RFLP, and many more. 1. Fast. Using a rapid spin-column format, the entire procedure takes less than 30 minutes. 2. High Purity of DNA. OD260/280 of purified DNA is generally > Compatible with many downstream applications such as PCR, restriction digestions, real-time PCR, multiplex PCR, RAPD, RFLP, AFLP, Southern Blotting and microsatellite analysis. 4. Suitable for a wide variety of plant species and tissue types including some recalcitrant specimens. BS425 BS626 2 RNase A (10mg/ml) 150ul 750ul PCL Solution 15ml 75ml PP Solution 2ml 10ml PB Solution 20ml 100ml Wash Solution 12ml 2X30ml Elution Buffer 5ml 25ml EZ-10 Spin Column ml Collection Tube Genomic DNA isolated from various plant species using the EZ-10 Plant DNA mini-prep kit. Lane 1-2: Nicotiana tabacum Lane 3-4: Arabidopsis thaliana Lane 5-6: Oryza sativa Lane 7-8: Platycladus orientalis Lane 9-10: Wisteria sinensis M: DNA marker 18

19 EZ-500 Spin Column Plant Genomic DNA Maxi-Preps Kit PT preps/ PT preps The kit is designed for the isolation of large quantities of genomic DNA from a wide variety of plant species. Samples may be fresh, frozen, or dried. DNA in plant tissues and cells are selectively adsorbed on the EZ-500 maxi-prep spin column, while other impurities such as proteins, salts, and lipids do not bind to the column and are washed away. Up to 500ug of plant DNA can be obtained per preparation. Average size of DNA purified using this procedure is ~30-50 kb. Purified genomic DNA can be used directly for PCR, Southern Blotting, RAPD, and more. 1. High Purity of DNA. OD260/280 of purified DNA is generally > Compatible with many downstream applications, including such as PCR, RAPD, RFLP, AFLP, Southern Blotting and microsatellite analysis. 3. Suitable for a wide variety of plant species and tissue types including some very recalcitrant specimens. Transportation: At ambient temperature. E.coli genomic DNA purified using the EZ-500 Spin Column Plant DNA Maxi-Preps Kit. Shown on a 0.8% agarose gel. M: DNA Marker 1-7: genomic DNA from E.coli M 96-Well Plate Plant Genomic DNA Mini-Preps Kit This kit provides a rapid and convenient high-throughput technique for the preparation of high quality genomic DNA from various plant species. DNA in a plant lysate is selectively adsorbed on each column of the plate while other impurities such as proteins, salts, and lipids are eliminated from the column. Each column can adsorb up to 20ug of DNA. No phenol/chloroform extraction or ethanol precipitation is required. Purified plant genomic DNA is kb, and is suitable for an array of downstream applications including PCR, Southern blotting, microsatellite analysis, AFLP, RFLP, and RAPD. 1. Fast. Using a spin column format, the entire procedure takes less than 2 hours. 2. High purity of DNA. OD260/280 of purified DNA is generally > Compatible with many downstream applications. 4. Suitable for a wide variety of plant species and tissue types including some very recalcitrant specimens. Universal Buffer PCB Universal Buffer BD Universal PW Solution Universal Wash Solution TE Buffer (ph 8.0) 96-Well EZ Plate 2 96-Well Collection Plate 6 96-Well Storage Plate 2 Sealing film 8 BS Plates 130ml 50ml 72ml 30ml 40ml Plant genomic DNA purified using the 96-well Plate DNA Mini-Prep Kit. 19

20 EZ-10 Spin Column Soil DNA Mini-Preps Kit This kit provides PCR-inhibitor free DNA through a convenient and rapid method for the detection of microorganisms from soil, sand and fecal samples. These samples are considered challenging as they contain rich humic acid that can interfere with the PCR reaction. The kit removes all traces of humic acid using an optimized buffer during the spin column procedure. Briefly, the soil sample is lysed using lysis buffer (Buffer SCL). DNA is then bound to an EZ-10 column in the presence of chaotropic salt. Under these conditions only DNA will bind to the membrane while most of the contaminating RNA, proteins, and cell debris are removed in the flow through. Purified inhibitor-free DNA is then eluted into ul of the provided TE buffer or water. Total genomic DNA can be isolated and purified from various microorganisms found in soil, including bacteria, fungi and algae. Purified humic acid-free DNA is of the highest quality and is fully compatible with PCR applications or other downstream applications. The molecular size of the purified DNA is around kb. Average DNA yields are 5-20ug per gram of soil sample. 1. Fast. Using a rapid spin column format, the entire procedure takes 30 minutes. 2. Versatile. All types of soil samples can be processed with this kit, including sand and fecal samples. 3. High Quality of DNA. OD260/OD280 of purified DNA is generally The purified DNA is almost free from all inhibitors including humic acid. 4. Economical ST82316 SCL Solution SP Solution SB Solution Wash Solution Elution Buffer 25ml 25ml 40ml 12ml 5ml Spin Column 50 2ml Collection Tube 50 DNA purified from soil samples. One-Tube Tissue DNA Extraction Kit This kit facilitates rapid isolation of genomic DNA from a wide array of sample types, including animal tissue, mouse tail and ear clips, human hair or saliva, and fish and insect tissue. There is no need for phenol extraction, overnight digestion, DNA precipitation or column purification; the lysate can be used in PCR directly. One-tube extraction kit minimizes the cross-contamination between samples. The procedure takes less than 15 minutes, and the kit is suitable for high throughput PCR screening of large numbers of samples. For other applications, Rapid Animal DNA Isolation Kit and EZ-10 Spin Column Animal DNA Mini-preps Kit (codes: BS427 AT4781; AT4780; BS428) are recommended. Samples recommended: cm mouse tail; or mm mouse ear punch; or 2-5mg piece of tissue; or 1-10 hairs with roots; or 20µl saliva; or 2-3 mm 2 piece of zebrafish fin. 1. Fast. The entire procedure takes less than 15 minutes. 2. High Quality of DNA. OD260/OD280 of purified DNA is generally Versatile. Compatible with tissues from mice, fish and insects, human hair, saliva, swab, blood clot and so on. 4. Easy to scale up. 5. Economic. BS Preps BS Preps Lysis-Buffer-T 20ml 100ml Proteinase K 2ml 10ml Universal Buffer NST 20ml 100ml 1 PCR amplification using DNA isolated by One-Tube Tissue DNA Extraction Kit as template. Lane 1-3: template DNA from Rat muscle Lane 4-6: template DNA from Rat liver 20