Office Chromatography
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1 Office Chromatography Gertrud Morlock & Wolfgang Schwack
2 Planar Chromatography 1938 TLC Thin-layer chromatography 1975 HPTLC High-performance TLC 2001 UTLC UltraTLC not known! J. Sherma, G. Morlock, J. Planar Chromatogr. 21 (2008)
3 The very first start
4 printing pictures on TLC foils
5 at a perfect spacial resolution Scans from 0,6 to 1,5 mm
6 ... or the magic dinner card for HPTLC 2006 The most frequently asked question at this evening was: What is the menu? It was always pointed to the fresh HPTLC plates on each dinner table It took a while until a scientist started to heat the HPTLC plate as a plate heater was noticed. Then, other followed to do the same. The sweetener sucrose was not in the meal, but on a ß-naphthol-impregnated plate the letters were still readable when visualized after 3 days of printing the diffusion was not caused due to the alcohol consumption of the scientists
7 ... definitely Chinese scientists managed it! We found the proof later in the Chinese web The personal label was also printed on a TLC foil The stained TLC foil
8 Can one use the printer for printing of reagents? First Conny Stiefel
9 What are the advantageous to given solutions? Our students spraying cabinet
10 Performance of the reagent printer Analysis of taurine in energy drinks Red Bull samples Calibration ng Printed zone Taurine Samples
11 Flexibility of the reagent printer Track specific Substance window specific In situ prechromatographic derivatization
12 Get married with a reagent printer G. Morlock, C. Stiefel, W. Schwack J. Liq. Chromatogr. Relat. Technol. 30 (2007)
13 CBS 100 (2008) 9
14 Again found in the Chinese web
15 Printing bacteria on the plate Vibrio Fischeri still alive congratulate:
16 Sometimes one should be few months earlier!
17 Our next working station Second Claudia Oellig
18 Printing samples quantitatively? Applicator Inkjet printer Piezoelectric dispenser Pneumatic spray Deposition volume nl nl µl Application linearity R 2 (max. %RSD) Precision %RSD ( S/N) Spots Bands Spots Bands (3.2%) (2.3%) (6.4%) (1.3%) n/a (5.1%) % (6) % (9) % (9) % (10) n/a % (21)
19 Office Chromatography = printer & scanner
20 UTLC plates G. Morlock, W. Schwack, TrAC, in preparation
21 UTLC plates UTLC Plate M-UTLC NS-UTLC Stationary phase Monolithic silica gel, 10 µm thick Nanostructured silica, 5 µm thick Monolithic R 2 Linearity" (max. %RSD) Nanostructured Precision %RSD ( S/N) LOQ range (amount/band) (1.3%) % (10) ng (1.3%) % (16) ng
22 Development within a minute
23 Ultrathin plate (UTLC)
24 Nanostructured UTLC plates Re-use of plate (5 x) Sample flow Solvent flow 20 samples/min 3 s/sample
25 by the nanostructure designers Jim Steven & Louis Bezuidenhout
26 Office Chromatography The whole concept is even more Miniaturized planar chromatography using office peripherals, G. Morlock, C. Oellig, L. Bezuidenhout, M. Brett & W. Schwack, Anal. Chem in print
27 Office Chromatography - next steps At its infancy we appreciate funding! Plate design in co-operation with the group of Prof. Dr. Brett Apparatus we appreciate co-operations! Hyphenations Reaching the water source you have to swim against mainstream. Konfuzius
28 Thanks to Prof. Dr. M. Brett Louis Bezuidenhout Steven Jim Prof. Dr. W. Schwack CAMAG, CH Merck, D Jeol, F Bruker Daltonics, D Claudia Oellig
29 News on Office Chromatography! HPTLC 2011, Basel : 6 th -8 th July
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