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1 Curriculum Vitae Identification Full Name: Miguel Ângelo Rodrigues Larguinho National Identity Card: Birth place: Almada Birth date: 17/09/1984 Nationality: Portuguese Marital status: Single Living Address: Rua Miguel Russel, Nº16-1ºB, Quinta da Marialva, CORROIOS Cell phone: Driving License: SE Academic Degree Title: Molecular and Cellular Biology Graduation Year: 2007 Classification: 16 out of 20 Academic Degree: Licenciatura (4-year degree) Institution: Faculty of Science and Technology/University NOVA de Lisboa Scientific and professional activities Period Category Institution April ongoing PhD student CIGMH/Life Science Dept. and REQUIMTE/ Chemistry Dept., Faculty of Science and Technology/University NOVA de Lisboa September 2009 March 2010 Research assistant CIGMH/Life Science Dept., Faculty of Science and Technology/University NOVA de Lisboa July 2008 July 2009 Research assistant CIGMH/Life Science Dept., Faculty of Science and Technology/University NOVA de Lisboa
2 Research interests Area of scientific activity: Bionanotechnology, nanodiagnostics, toxicology, HPLC, MALDI- TOF-MS. Fields of specialisation: Bionanotechnology, gold nanoparticles, detection of biological molecules, nanodiagnostics, DNA adducts, MALDI-TOF-MS, toxicology. Present research interests: Bionanotechnology, detection of biological molecules, nanodiagnostics, biosensors, therapy, nanotoxicology, genetic and aquatic toxicology, cell biology and genetics, mass spectrometry. Publications Scientific Papers M. Larguinho, S. Santos, J. Almeida, P.V. Baptista (2014) DNA adduct identification using gold-aptamer nanoprobes. To be Submitted. M. Larguinho, D. Correia, M.S. Diniz, P.V. Baptista (2014) Evidence of one-way flow bioaccumulation of gold nanoparticles across two trophic levels. Journal of Nanoparticle Research. Submitted for revision. D.V. Luis, J. Silva, A.I. Tomaz, R.F.M. de Almeida, M. Larguinho, P.V. Baptista, L.M.D.R.S. Martins, T.F.S. Silva, P.M. Borralho, C.M.P. Rodrigues, A.S. Rodrigues, A.J.L. Pombeiro, A.R. Fernandes. Insights into the mechanisms underlying the anti-proliferative potential of a Co(II) coordination compound bearing 1,10-phenanthroline-5,6-dione: DNA and protein interaction studies. Journal of Biological Inorganic Chemistry. Just Accepted. M. Larguinho, P.M. Costa, G. Sousa, M.H. Costa, M.S. Diniz, P.V. Baptista (2014) Histopathological findings on Carassius auratus hepatopancreas upon exposure to acrylamide: correlation with genotoxicity and metabolic alterations. Journal of Applied Toxicology (doi: /jat.2936). J. Conde, M. Larguinho, A. Cordeiro, L.R. Raposo, P.M. Costa, S. Santos, M.S. Diniz, A.R. Fernandes, P.V. Baptista (2014) Gold-nanobeacons for gene therapy: evaluation of genotoxicity, cell toxicity and proteome profiling analysis. Nanotoxicology 8: (doi: / ).
3 M. Larguinho, J.L. Capelo, P.V. Baptista (2013) Fast nucleotide identification through fingerprinting using gold nanoparticle-based surface-assisted laser desorption/ionisation. Talanta 105: (doi: /j.talanta ). M. Larguinho and P.V. Baptista (2012) Gold and silver nanoparticles for clinical diagnostics - From genomics to proteomics. Journal of Proteomics 75: (doi: /j.jprot ). C. Nuñez, J. Fernandez-Lodeiro, M.S. Diniz, M. Larguinho, J.L. Capelo, C. Lodeiro (2011) Synthesis and photophysical studies of two luminescent chemosensors based on cathecol and 8- Hydroxyquinolone chromophores, and their complexes with group 13 metal ions. Inorganic Chemistry Communications 14: (doi: /j.inoche ). G. Doria, M. Larguinho, J.T. Dias, E. Pereira, R. Franco, P.V. Baptista (2010) Gold-silver alloy nanoprobes for one-pot multiplex DNA detection. Nanotechnology 21: (doi: / /21/25/255101). M. Larguinho, H.M. Santos, G. Doria, H. Scholz, P.V. Baptista, J.L. Capelo (2010) Development of a fast and efficient ultrasonic-based strategy for DNA fragmentation. Talanta 81: (doi: /j.talanta ). Book chapters M. Larguinho, S. Figueiredo, A. Cordeiro, F.F. Carlos, M. Cordeiro, P. Pedrosa, P.V. Baptista (2014) Nanoparticles for diagnostic and imaging. Frontiers in Nanomedicine vol I. Bentham Science. To be Submitted. M. Larguinho, J.L. Capelo, P.V. Baptista (2013) Nanoparticles for mass spectrometry applications. Handbook of Nanoparticles, Synthesis, Functionalization and Surface Treatment. Springer-Verlag GmbH, Germany. Submitted for consideration. Communications Oral communications
4 G. Doria, J. Conde, M. Larguinho, P. Baptista (2010) Multiplex genetic characterization via noble metal nanoprobes. Trends in NanoTechnology th -10 th September Braga, Portugal (oral presentation by GD). G. Doria, M. Larguinho, J.T. Dias, E. Pereira, R. Franco, P. Baptista. AuAg-alloy-nanoprobes for specific nucleic acid detection. Nanotech Conference & Expo June 21 st -24 th Anaheim, USA (oral presentation by GD). M. Larguinho. Bionanotechnology for nanodiagnostics the emergence of SALDI-TOF for biological analysis. XI Jornadas de Biologia Aplicada. 28 th -30 th April Braga, Portugal (oral presentation by ML). Posters in conferences M. Larguinho, A. Cordeiro, P.M. Costa, M.S. Diniz, P.V. Baptista (2013) Histopathologic effects of waterborne acrylamide in Mytilus galloprovincialis. Microscopy in Research th -10 th December Caparica, Portugal. J. Conde, M. Larguinho, A. Cordeiro, L.R. Raposo, P.M. Costa, S. Santos, M.S. Diniz, A.R. Fernandes, P.V. Baptista (2013) Gold-Nanobeacons for gene silencing: evaluation of the effect and toxicity of a new nanotheranostics tool. European Human Genetics Conference th - 11 th June Paris, France. M. Larguinho, D. Correia, M.S. Diniz, P.V. Baptista (2013) Gold nanoparticle bioaccumulation and nanotoxicity in marine environments. II National Encounter in Nanotoxicology. 2 nd -3 rd April Lisbon, Portugal. A. Cordeiro, M. Larguinho, J. Conde, L.R. Raposo, P.M. Costa, S. Santos, M.S. Diniz, A.R. Fernandes, P.V. Baptista (2013) Analysing the toxicological potential of an antisense Aunanobeacon system for gene therapy. II National Encounter in Nanotoxicology. 2 nd -3 rd April Lisbon, Portugal. M. Larguinho, J.L. Capelo, P.V. Baptista (2012) Colorimetric DNA adduct identification using aptamer-gold nanoprobes. GOLD The 6th International Conference on gold science, technology and its applications. 5 th -8 th September Tokyo, Japan.
5 M. Larguinho, J.L. Capelo, P.V. Baptista (2012) DNA adduct identification using gold nanoparticles for surface-assisted laser desorption/ionisation time-of-flight mass spectrometry. XXXVII Jornadas Portuguesas de Genética. 28 th -30 th May Lisbon, Portugal. M. Larguinho, C. Lodeiro, J.L. Capelo, P. Baptista (2011) Nucleotide identification using nanoparticles for surface-assisted laser desorption/ionisation. International workshop on nanoplasmonics for energy and the environment. 8 th -10 th June Sanxexo, Spain. G. Doria, J. Conde, M. Larguinho, P. Baptista (2010) Multiplex genetic characterization via noble metal nanoprobes. Trends in NanoTechnology th -10 th September Braga, Portugal. M. Larguinho, G. Doria, J.L. Capelo, P.V. Baptista (2010). Speeding up DNA purification for gold nanoprobe-based detection assays. Trends in Nanotechnology th -10 th September Braga, Portugal. M. Larguinho, H.M. Santos, G. Doria, J.L. Capelo, P.V. Baptista (2009). Ultrasonic fragmentation of DNA and its importance in sample treatment for detection of specific nucleotide sequences. Microbiotec th -30 th November Vilamoura, Portugal. Maria João Barreira, Cristina M. M. Almeida, Miguel Larguinho, Paula Alvito (2008). Patulin in baby foods: optimization and validation of the SPE-HPLC-UV method. The World Mycotoxin Forum. 17 th -18 th November Noordwijk, The Netherlands. M. Larguinho, B. Pedras, T. Avilés, M.M.M. Raposo, B. Covelo, C. Lodeiro (2006). Rigid and Flexible Schiff-base Bithiophene-Crown Ether ligands for metal ion detection. Synthesis and characterization. XX National Encounter, Portuguese Chemistry Society. 14 th -16 th December Caparica, Portugal. B. Pedras, M. Larguinho, C. Lodeiro, A.J. Parola, T. Avilés, M. Raposo, R.F. Batista, S.P. Costa (2006). Synthesis of Bithiophene Derivatives as Emissive Devices. 4 th National Encounter of REQUIMTE. 31 st March - 1 st April Fátima, Portugal. Participation in research projects Research assistant: Project PTDC/BIO/66514/2006: "Sensitive and selective detection of DNA/RNA based on functionalised gold nanoparticles application to the detection of
6 pathogen agents; detection of mutations and RNA quantification. Supervision by Prof. Pedro Viana Baptista. Research assistant: Project PTDC/SAU-BEB/66511/2006: Nanodiagnostics: gold nanoprobes for molecular identification - application to the detection of pathogen agents; detection of mutations and RNA quantification. Supervision by Prof. Pedro Viana Baptista. Other skills/activities - Scientific training in the laboratory of nutrition, toxicology and contaminants (INSA). Development of analytical methods (HPLC and LC-MS) for identification and quantification of the mycotoxin patulin in apple products. Supervision: Dr. Paula Alvito, MSc Maria Barreira. - Scientific training in the plant genetics laboratory (ISA - UTL). Learning of cell biology techniques and fluorescence microscopy towards the study of cell epigenetic machinery and chromatin organisation patterns in several Arabidopsis tissues. Supervision: Prof. Nuno Neves, Prof. Wanda Viegas and MSc Márcia Rosa. - Scientific training in the photochemistry laboratory (FCT - UNL). Chemical synthesis and characterisation of organic compounds for development of chemosensors. Supervision: Prof. Carlos Lodeiro Espiño. - Workshop Advanced course in microalgae culturing and maintenance. 9 th -13 th September BioPlant, University of Aveiro, Portugal. - Workshop Introduction to forensic sciences. 29 th September Coimbra, Portugal. - Level I trainer/coach in shaolin Kung Fu and Tai Chi Chuan (YMAA Portugal). Language Native language: Portuguese Other languages (according to Common European Framework of Reference): Language Reading Writing Conversation English Proficient Proficient Proficient Spanish Basic Basic Independent
7 French Basic Basic Basic Japanese Basic Basic Basic
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