Needle Free Serum. NF-13 Complex: the needle-free collagen and elastin filler
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1 Needle Free Serum : the needle-free collagen and elastin filler Needle Free Serum is clinically proven to deliver equivalent results to one injection of collagen filler in just two weeks of regular use. Upon aging, cells are no longer able to produce collagens and elastin effectively. By providing a new source of bio-energy this production is reactivated. boosts senescent fibroblasts to: 19% Increase in elastin production after 11 days 179% Increase in collagen I production, and 19% Increase in collagen III production after 11 days 5% decrease in skin laxity after days Improved skin clarity for rosacea patients due to a reduction in capillary branching, length and clarity within 1 weeks Firmness increased by 13 times in 15 days Reduces wrinkles depth by 15%, and volume by 13% in only 15 days % inhibition of MMP enzyme activity (types 1 and ) 5% increase in hyraluronic acid synthesis in vitro tests Improvements sustained after two months of treatment
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3 Biological activity Production of total collagen, collagens I and III (ex vivo tests) The action of on total collagen production was evaluated on skin explants from a 7 years old donor. Skin explants were either untreated or treated with.5% of at day, day 1, day, day and day. Explants were then sampled and analyzed at day, day and day 11. Total collagen was detected with staining assessed by microscopical observation and image analysis. Total collagen Results: used at.5% increases the quantity of total collagen by.% in 11 days. Percentage by total collagen (%) %.5% 75 Day 1 Day Improvement of fibroblasts vitality Production of Pro-collagen I in vitro Improvement of mean viability (% improvement versus untreated cells) 17 3 Concentration of 5 Pro-collagen I in ng/ml (% improvement versus untreated cells) 3 1 Young fibroblasts Aged fibroblasts.%.% %.1%.5% % % of Results: dramatically increases the vitality of young and aged fibroblasts. Aged fibroblasts are enabled to function property again. Results: increases the synthesis of Pro-collagen I in a dose dependent way. Aged fibroblasts show even better ratio of production compared to young fibroblasts. p<.1 compared to untreated, Student t-test p<.1 compared to untreated, Student t-test MMP enzymatic activity (% control) Inhibition of MMP Enzymatic Activity NF-13 concentration MMP-9 MMP- Improvement factor Mean reduction in mild to moderate rosacea from baseline at 1 weeks a Clarity b branching c length a p<.3 - b p<. - c p<. Placebo NF-13 inhibits more than % of MMP enzymes activity (Types and 9). The MMP-1 enzyme is also inhibited by NF volunteers (7 enrolled) with mild to moderate rosacea, visible telangiectases. Randomized split-face, double-blind trial, 1 weeks with a 5% cream or placebo.
4 Biological activity studying the total collagen, two different types of collagens were quantitatively evaluated by immunostaining: collagen I (in green fluorescent), and collagen III (in dark rose), also known as the youth collagen. Collagen type I Collagen type III (Youth Collagen) Percentage by collagen I (%) % Percentage by collagen 3 III (%) +19% 1 1 Day Day Day Day p<.1 compared to untreated, Student t-test p<.1 compared to untreated, Student t-test Untreated.5% Untreated.5% Results: at.5% significantly increases collagen I and collagen III synthesis by +179% and +19% respectively after 11 days, versus untreated. Upon aging, the ratio of collagen I / collagen III is increased. With Neodermyl at.5%, this ratio is decreased to the level of a younger skin: it moves from. to.55. Production of elastin (ex vivo tests) The action of on tropoelastin and elastin production was evaluated by following the same protocol as for collagen I and III. Tropoelastin (in green fluorescent) and elastin (in green fluorescent) were detected by immunostaining assessed by microscopical observation and image analysis. Percentage 1 by 1 tropoelastin 1 (%) Tropoelastin Untreated Day Elastin Percentage 1 1 by +57% 1 elastin (%) %.5% Day Day Day Day Untreated Day.5%.5%.5% p<.1 compared to untreated, Student t-test p<.5 compared to untreated, Student t-test Results: significantly increases tropoelastin and elastin production by +57% and +19% respectively after 11 days, versus untreated.
5 Efficacy Fast improvement of firmness and elasticity (clinical) The two parameters R and R5 were measured by Cutometry to evaluate the performance of at 1% on firmness and elasticity. Improvement of skin firmness (R) (% versus T) Firmness (R) x13 x1 Elasticity (R5) Improvement 1 of skin elasticity (R5) 1 (% versus T) +% 1 +% Day 15 Day Day 15 Day Placebo 1% Placebo 1% p<.1 compared to placebo, Student t-test p<.1 compared to placebo, Student t-test p<.1 compared to T, Student t-test Results: Using at 1% increases firmness by 13 times and elasticity by 1. times in only 15 days. These improvements of skin parameters are sustained after two months of treatment. Wrinkles profiles confirm the benefits of using at 1%. 15 days, the results are outstanding: Wrinkle profile Untreated Day Volunteer number 13-7 years old - crow's feet area Wrinkle profile 1% Day 15 Results: The skin is smooth and wrinkles are visibly reduced after only 15 days of treatment with use at 1%. delivers equivalent results to one injection of collagen filler in just weeks: -15% of wrinkles reduction (source American Society of Plastic Surgeons 13).
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