API 5000 LC/MS/MS SYSTEM. Sensitivity, ruggedness, and simplicity. Together at last.

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1 API 5000 LC/MS/MS SYSTEM Sensitivity, ruggedness, and simplicity. Together at last. API 5000 LC/MS/MS SYSTEM

2 The API 5000 LC/MS/MS System is one of the most sensitive triple quadrupole mass spectrometers for complex bioanalytical samples. Designed to deliver the lowest limits of detection for the most demanding DMPK and ADME studies, the API 5000 System combines new QJet Ion Guide technology with the proven Turbo V Ion Source and a powerful new generation of Analyst and MultiQuant Software. The result is a robust, versatile, high-throughput system with industry-defining quantitative performance, reliability, and ease of use.

3 A new benchmark for quantitative small molecule analysis Ideal for small molecule analysis The API 5000 LC/MS/MS System gives you exceptional accuracy and precision for quantitative analysis. It also offers linearity over a wide dynamic range and exceptional sensitivity for routine quantitation of low abundance compounds in complex matrices with greater signalto-noise than even the API 4000 System. Intensity, cps Time, min This chromatogram acquired at unit resolution of both Q1 and Q3 demonstrates outstanding S/N for 10 fg of buspirone injected on-column. The data are taken from one of the eight replicate injections of the lowest concentration in the calibration curve shown in the figure at right. At this level, the %CV is an impressive 3.5. The API 5000 LC/MS/MS System is backed by an unmatched track record of experience and performance in pharmaceutical development applications. It is one of the most sensitive triple quadrupole systems we have ever produced and the most powerful.

4 Uncompromised performance and ease of use Get expert results even if you re not an expert Not only is the API 5000 one of the most powerful triple quads available, it is also one of the easiest to use. From automated methods development to quick, simple routine maintenance, the API 5000 System makes it easy for you to get the answers you need, even if you are just getting into mass spectrometry. Unattended, high-throughput operation The API 5000 System is engineered for continuous duty operation in high-throughput regulated laboratories. With a full complement of advanced automation features, it fits seamlessly into your workflow and requires limited user intervention. r= Area, counts Concentration, ng/ml Response = internal standard corrected d5-alprazolam 1 pg/µl MRM transition CV 2.5% = Alprazolam 2.5 pg/µl MRM transition CV 3.1% Internal Standard Area Ratio Injection Number Excellent linearity over a wide dynamic range is illustrated in this calibration curve of eight replicate injections at each concentration of buspirone. From the table, the statistics and accuracy demonstrate the unparalleled consistency and reproducibility required for quantitation in regulated environments. Over two days and 500 plasma sample injections, the API 5000 System demonstrates excellent reproducibility and stability for the compound and internal standard responses.

5 High throughput for the networked lab AB SCIEX combines one of the world s most sensitive mass spectrometers with the industry s leading software for robust, high performance quantitation. Analyst Software delivers a new standard in compliance, throughput, and ease of use. The best quantitation tools for rapid results: reduce your integration, method development, and data review time Rapid acquisition method development: automate your compound optimization and tuning routines Intelligent integration: new parameter-free algorithms reduce time spent revising and reviewing results The most comprehensive suite of features available for 21 CFR Part 11 compliance: complete, role-based security, auditing, and electronic records Centralized security management: power and flexibility in one easy-to-use console provide the finest security model for regulated labs Powerful auditing features: complete, easily managed auditing tools give you project-based auditing on your terms for all levels of compliance Direct network acquisition: eliminate manual file management and enhance your data security by acquiring data directly over your network Support for your workflow: from LIMS to processing tools, Analyst Software has the flexibility for fast integration into your lab environment Advanced Peak Deconvolution consistently integrates poorly resolved peaks across the concentration range by modeling the actual peak shape from the raw data. This allows the software to more reliably define the peak boundaries and provide more accurate integration. 2.5 pg/ul 5 pg/ul 12.5 pg/ul Peak of Interest Unresolved Interference Shoulder Tail

6 Technology in a class by itself Innovative Turbo V Ion Source Efficiently ionizes compounds and virtually eliminates crosscontamination, even with large sample loads. Embedded ceramic heater technology and improved gas dynamics contribute to the system s low detection limits and enable high-sensitivity quantitation over the wide range of flow rates used in pharmaceutical analyses. Quick-change ESI and APCI probes let you switch between ionization modes in seconds. Turbo V Ion Source Curtain Plate Orifice IQ1 Q1 Turbo V Source QJet Ion Guide Q0 High Pressure Cell ST ST2 IQ2 Curtain Gas Interface

7 Proprietary Curtain Gas Interface Reduces the need for routine maintenance and ensures maximum uptime and productivity by protecting the interface region and quadrupole analyzer from contamination. Revolutionary new QJet Ion Guide Replaces the skimmer in earlier triple quad designs, dramatically improving sensitivity. It captures more ions that enter the instrument, efficiently separates them from neutrals, and focuses them into Q0. Curtain Gas Interface Q2 Q3 CEM LINAC Collision Cell IQ3 ST3 EXIT LENS DETECTOR

8 You invest in our technology. We invest in your success. As the world leader in mass spectrometry, AB SCIEX solutions are backed by the industry s most extensive service and support organization. With a network of service professionals, experienced compliance specialists, and over 150 PhD application scientists worldwide, we are dedicated to supporting your technical needs and helping you get the most out of your AB SCIEX systems. AB SCIEX service professionals are recognized as the most highly qualified in the industry. They are certified on our instrument platforms through a rigorous 4-step certification program, with re-certification occurring every two years. This award-winning program helps to ensure that you receive the most efficient, highest-quality, and most up-to-date service available for AB SCIEX products and technology. Choose from flexible service plans and a variety of services for the right level of support for your laboratory s needs and budget. Our customer support network is available to provide expert assistance in the use and application of AB SCIEX products through a comprehensive range of services, including application support, technical service, and training. Whether you access our service and support team by phone, , on-site visits, or through our innovative remote monitoring technology, you can be confident that the AB SCIEX organization will be there for you. For more information, visit For Research Use Only. Not for use in diagnostic procedures AB SCIEX. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. AB SCIEX is being used under license /2010 Headquarters 353 Hatch Drive Foster City CA USA Phone International Sales For our office locations please call the division headquarters or refer to our website at