Porto, December 2013 ABSTRACT. Curriculum Vitae Ana Raquel Pinho Freitas

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1 Curriculum Vitae Ana Raquel Pinho Freitas Porto, December 2013 ABSTRACT Ana R. Freitas Fernandes, 32 years, natural of Porto, graduated in Pharmaceutical Sciences by the Faculty of Pharmacy, University of Porto in She obtained a PhD degree in Microbiology with maximum classification by the Faculty o f Pharmacy/University of Porto in close collaboration with Hospital Universitario Ramón y Cajal, Madrid/Spain, in She has published 16 papers in international peer review indexed journals with an accumulated impact factor of 64 (average of 4.3/publication) and 173 citations (average of 12/publication; current Index H of 7). 44 researchers from 9 countries were co- authors in these papers. She has additionally published one book chapter with authors international leaderships in the topic, 3 papers in non- indexed national journals, 32 abstracts from considered conference proceedings in international peer review journals, and 24 nucleotide sequences. She participated in 9 Research Projects, including 4 funded by the European Union, and has been recently granted by the European Society of Clinical Microbiology and Infectious Diseases with an ESCMID Research Grant as Principal Investigator. She has 87 communications (68 posters/19 oral presentations) presented in national (13) and international (74) conferences. She attended to 44 scientific events, which occurred in different EU countries and the USA (24) or in Portugal (20). She is reviewer for international journals highly recognized in the field of Microbiology and antibiotic resistance (e.g. Journal of Antimicrobial Chemotherapy, IF 5,068). She was invited as keynote speaker for 2 national lectures and as examiner for a th master thesis. She was one of the main local organizers in the event 8 ESCMID Summer School (Porto, 2009) annually organized by ESCMID. During her PhD, she supervised several undergraduate students in the Master of Pharmaceutical Sciences providing their integration in the ongoing research projects. She received 8 awards related with the high scientific quality of the communications presented in foreign countries. Her PhD research work has focused on the molecular epidemiology and ecology of multidrug- resistant Enterococcus strains recovered from different origins and countries, having greatly contributed to the better understanding of the population structure and the ecology of vancomycin- resistant plasmids. Achievement of different technical skills and competences include processing of clinical/animal/food samples, antibiotic susceptibility methods, molecular methods (e.g. PCR techniques, pulsed- field gel electrophoresis, multilocus sequence typing, southern blot) and analysis of and DNA fingerprinting data using different software programs and databases (BLAST, ClustalW, Bionumerics). sequence During her scientific formation, she has been trained to test, optimize and adjust laboratory procedures, to write her own manuscripts, and had the opportunity to help in the design and preparation of scientific projects/reports. The participation in different international and national scientific meetings, the active collaboration with different leaderships in different fields contributed to acquire scientific maturity and independence skills. international 1

2 Personal details Name: Ana Raquel Pinho Freitas Address: Rua Adélia Ferreira Santos Carvalho, n.º 112, Maia, Portugal Birth date: 14 January 1981 Nationality: Portuguese Phone: E- mail: Academic qualification PhD in Microbiology, Faculty of Pharmacy, University of Porto. Porto, Portugal. Thesis: Ecology and evolution of Antimicrobial Resistance in Enterococcus: A multilayered molecular approach with emphasis in plasmid diversity. In collaboration with Hospital Ramón y Cajal, Madrid, Spain Graduation in Pharmaceutical Sciences, Faculdade de Farmácia da Universidade do Porto. Porto, Portugal. Actual position Researcher collaborator of REQUIMTE ( Department of Microbiology. Faculty of Pharmacy, University of Porto, Portugal. List of publications 1. Scientific papers in international peer- reviewed journals 1. Silveira E, Freitas AR, Antunes P, Barros M, Campos J, Coque TM, Peixe L, Novais C Co- transfer of resistance to high concentrations of copper and first line antibiotics among Enterococcus from different origins (human, animal, environment and food) and clonal lineages. Journal of Antimicrobial Chemotherapy. In press. 2. Novais C, Freitas AR, Silveira E, Antunes P, Silva R, Coque TM, Peixe L Spread of multidrug- resistant Enterococcus to animals and humans: an underestimated role for the pig farm environment. Journal of Antimicrobial Chemotherapy. 68(12): Freitas AR, Novais C, Tedim AP, Francia MV, Baquero F, Peixe L, Coque TM Microevolutionary Events Involving Narrow Host Plasmids Influences Local Fixation of Vancomycin- Resistance in Enterococcus. PLoS One. 8(3):e Novais C, Freitas AR, Silveira E, Baquero F, Peixe L, Roberts AP, Coque TM A tet(s/m) hybrid from CTn6000 and CTn916 recombination. Microbiology. 158(Pt 11): Novais C, Freitas AR, Silveira E, Baquero F, Peixe L, Roberts AP, Coque TM Different genetic supports for the tet(s) gene in enterococci. Antimicrobial Agents and Chemotherapy. 56(11): Valdezate S, Miranda C, Navarro A, Freitas AR, Cabrera J, Carrasco G, Coque TM, Jiménez- Romano E, Saéz- Nieto JA Clonal outbreak of multidrug- resistant ST17 Enterococcus 2

3 faecium harbouring an Inc18- like::tn1546 plasmid in a haemo- oncology ward of a Spanish hospital. Journal of Antimicrobial Chemotherapy. 67(4): Freitas AR, Coque TM, Novais C, Hammerum AM, Lester CH, Jensen LB, Zervos MJ, Donadedian S, Francia MV, Baquero F, Peixe L Human and swine hosts share vancomycin- resistant Enterococcus faecium CC17, CC5, and Enterococcus faecalis CC2 clonal clusters harboring Tn1546 on indistinguishable plasmids. Journal of Clinical Microbiology. 49(3): Freitas AR, Novais C, Correia R, Monteiro M, Coque TM, Peixe L Non- susceptibility to tigecycline in enterococci from hospitalized patients, food products and community sources. International Journal of Antimicrobial Agents. 38: Werner G, Freitas AR, Coque TM, Sollid JE, Lester C, Hammerum AM, Garcia- Migura L, Jensen LB, Francia MV, Witte W, Willems RJ, Sundsfjord A Host range of enterococcal vana plasmids among Gram- positive intestinal bacteria. Journal of Antimicrobial Chemotherapy. 66(2): Laverde Gomez JA, van Schaik W, Freitas AR, Coque TM, Weaver KE, Francia MV, Witte W, Werner G A multiresistance megaplasmid plg1 bearing a hyl Efm genomic island in hospital Enterococcus faecium isolates. International Journal of Medical Microbiology. 301(2): Macedo AS, Freitas AR, Abreu C, Machado E, Peixe L, Sousa JC, Novais C Characterization of antibiotic resistant enterococci from untreated waters for human consumption in Portugal. International Journal of Food Microbiology. 145(1): Freitas AR, Tedim AP, Novais C, Ruiz- Garbajosa P, Werner G, Laverde- Gomez JA, Cantón R, Peixe L, Baquero F, Coque TM Global spread of the colonization- virulence hyl Efm gene in megaplasmids of CC17 Enterococcus faecium polyclonal sub- cluster. Antimicrobial Agents and Chemotherapy. 54(6): Ratkai C, Peixe LV, Grosso F, Freitas AR, Antunes P, Fodor E, Hajdú E, Nagy E Successful application of the DiversiLab repetitive- sequence- based PCR typing system for confirmation of the circulation of a multiresistant Pseudomonas aeruginosa clone in different hospital wards. Diagnostic Microbiology and Infectious Disease. 67(2): Freitas AR, Novais C, Ruiz- Garbajosa P, Coque TM, Peixe L Dispersion of multidrug resistant Enterococcus faecium belonging to major clonal complexes in different Portuguese settings. Applied and Environmental Microbiology. 75(14): Freitas AR, Novais C, Ruiz- Garbajosa P, Coque TM, Peixe L Clonal expansion within CC2 and dissemination of vancomycin- resistant plasmids in different Enterococcus faecalis clonal lineages of Portugal. Journal of Antimicrobial Chemotherapy. 63(6): Novais C, Freitas AR, Sousa JC, Baquero F, Coque TM, Peixe L Diversity of Tn1546 and its role in the dissemination of vancomycin- resistant enterococci in Portugal. Antimicrobial Agents and Chemotherapy. 52(3): Scientific papers in Portuguese journals Silveira E, Gomes F, Freitas AR, Silva R, Sousa JC, Coque TM, Peixe L, Novais C High prevalence of tcrb, a copper resistance gene, in antibiotic- resistant enterococci from swine and piggeries environment. Revista da Faculdade de Ciências da Saúde, Universidade Fernando Pessoa. 3

4 Silveira E, Freitas AR, Peixe L, Novais C Environmental spread of antibiotic molecules, antibiotic resistant bacteria and genes: jigsaw pieces of a public health problem. Revista da Faculdade de Ciências da Saúde, Universidade Fernando Pessoa. Nº.6: Nogueira A, Dias A, Silva R, Freitas AR, Sousa JC, Peixe L, Novais C Contribuição das suiniculturas na selecção e disseminação de Enterococcus spp resistentes às tetraciclinas. Revista da Faculdade de Ciências da Saúde, Universidade Fernando Pessoa. Nº.5: Book Chapters Coque TM, Freitas AR, Novais C, Peixe L, Baquero F Mobile Genetic Elements and Lateral Genetic Transfer in Enterococci. In: Enterococcus and Safety. Series: Advances in Food Safety and Food Microbiology. Semedo- Lemsaddek T, Barreto- Crespo MT, Tenreiro R (eds). Nova Publishers. 4. Abstracts submitted to ECCMID and published in the Journal Clinical Microbiology and Infection 1. Freitas AR, Tedim AP, Novais C, Ruiz- Garbajosa P, Peixe L, Baquero F, Coque TM Global dissemination of vancomycin- resistant VanB Enterococcus faecium causing outbreaks in different countries is mainly associated with chromosomal Tn1549/5382- like platforms. Clinical Microbiology and Infection. 18 (Issue S3): S329 (Abstr. P1290). 2. Novais C, Antunes P, Fonte J, Campos J, Silveira E, Grosso F, Machado E, Freitas AR, Silva R, Coque TM, Peixe L Occurrence of antibiotic- resistant enterococci from antibiotic- free trout aquacultures. Clinical Microbiology and Infection. 18 (Issue S3): S45 (Abstr. O302). 3. Silveira E, Freitas AR, Antunes P, Coque TM, Peixe L, Novais C Spread of large conjugative plasmids carrying antibiotic, copper and mercury resistance genes among Enterococcus from different sources. Clinical Microbiology and Infection. 18 (Issue S3): S447 (Abstr. P1631). 4. Antunes P, Campos J, Novais C, Machado E, Mourão J, Grosso F, Quinteira S, Freitas AR, Coque TM, Peixe L Aquacultures as reservoirs of pathogenic bacteria and clinically relevant antibiotic resistance genes. Clinical Microbiology and Infection. 18 (Issue S3): S448 (Abstr. P1632). 5. Freitas AR, Ruiz- Garbajosa P, Francia MV, Novais C, Zervos MJ, Donabedian S, Peixe L, Baquero F, Coque TM Spread of vancomycin- resistance among Enterococcus faecalis: the influence of pad1 and Inc18- plasmids ( ). Clinical Microbiology and Infection. 14 (Issue S4): S59 (Abstr. O301). 6. Tedim AP, Freitas AR, Baquero F, Coque TM Fitness cost of widely disseminated conjugative plasmids carrying Tn1546- vana among Enterococcus faecium and Enterococcus faecalis. 14 (Issue S4): S43 (Abstr. O214). 7. Quinteira S, Novais C, Antunes P, Campos J, Freitas AR, Abdukadir A, Monteiro N, Peixe L Tracking antibiotic resistance along the Silk Road. Clinical Microbiology and Infection. 14 (Issue S4): S58 (Abstr. O300). 8. Silveira E, Freitas AR, Coque TM, Peixe L, Novais C Co- dissemination of antibiotic and copper resistance genes in large conjugative plasmids of enterococci from different ecological niches. Clinical Microbiology and Infection. 14 (Issue S4): S (Abstr. R2336). 9. Novais C, Escada R, Freitas AR, Silveira E, Coque TM, Peixe L High incidence of horizontal transfer of ampicillin resistance (pbp5) among CC17 Enterococcus faecium. Clinical Microbiology and Infection. 14 (Issue S4): S129 (Abstr. P619). 10. Tedim AP, Miranda AC, Ruiz- Garbajosa P, Freitas AR, Francia MV, Baquero F, Coque TM Molecular characterisation of Enterococcus faecalis plasmids from clinical isolates in Spain. Clinical Microbiology and Infection. 14 (Issue S4): S43 (Abstr. O214). 11. Freitas AR, Tedim- Pedrosa A, Novais C, Peixe L, Baquero F, Coque TM Diversity of hyl plasmids among international CC17 Enterococcus faecium strains, Clinical Microbiology and Infection. 16 (Issue s2): S274- S275 (Abstr. 1008). 4

5 12. Freitas AR, Novais C, Cunha A, Silveira E, Peixe L, Coque TM Plasmid analysis of vancomycin- resistant enterococci isolates from Portuguese poultry and healthy humans. Clinical Microbiology and Infection. 16 (Issue s2): S275 (Abstr. 1009). 13. Novais C, Freitas AR, Baquero F, Peixe L, Roberts AP, Coque TM Diversity of CTn6000 among enterococci from different ecological niches. Clinical Microbiology and Infection. 16 (Issue s2): S273 (Abstr. 1004). 14. Silveira E, Freitas AR, Coque TM, Peixe L, Novais C Co- dispersion of copper and antibiotic resistance genes among enterococci from different ecological niches, Portugal. Clinical Microbiology and Infection. 16 (Issue s2): S245 (Abstr. 921). 15. Antunes P, Mourão J, Freitas AR, Peixe L Population structure of multidrug- resistant non- typhoidal Salmonella enterica isolates from Portugal. Clinical Microbiology and Infection. 16 (Issue s2): S371- S372 (Abstr. 1315). 16. Freitas AR, Novais C, Silva RM, Coque TM, Peixe L International spread of vancomycin- resistant Enterococcus faecium (CC17, CC5) and Enterococcus faecalis CC2 among animals and humans. Clinical Microbiology and Infection. 15 (Issue s4): S213 (Abstr. 840). 17. Freitas AR, Novais C, Francia MV, Peixe L, Coque TM Plasmid analysis of vancomycin- resistant Enterococcus faecium isolates from hospitals and aquatic environments in Portugal ( ). Clinical Microbiology and Infection. 15 (Issue s4): S213- S214 (Abstr. 841). 18. Novais C, Freitas AR, Barros C, Baquero F, Peixe L, Coque TM Portuguese healthy human volunteers are reservoirs of conjugative transposons associated with tetracycline and/or erythromycin resistance. Clinical Microbiology and Infection. 15 (Issue s4): S247 (Abstr. 948). 19. Novais C, Freitas AR, Baquero F, Peixe L, Coque TM Spread of EfcTn1 in enterococci from different ecological niches. Clinical Microbiology and Infection. 15 (Issue s4): S246- S247 (Abstr. 947). 20. Novais C, Freitas AR, Francia MV, Peixe L, Baquero F, Coque TM Characterization of Gram- positive conjugative transposons among international epidemic vancomycin- resistant isolates of Enterococcus faecalis and Enterococcus faecium. Clinical Microbiology and Infection. 15 (Issue s4): S247 (Abstr. 949). 21. Tedim- Pedrosa A, Freitas AR, Ruiz- Garbajosa P, Baquero F, Coque TM Plasmid analysis of ampicillin- resistant and ampicillin- susceptible Enterococcus faecium causing bacteraemia in a Spanish university hospital ( ). Clinical Microbiology and Infection. 15 (Issue s4): S211- S212 (Abstr. 835). 22. Freitas AR, Novais C, Ruiz- Garbajosa P, Coque TM, Peixe L Population Structure of Enterococcus isolates from Portugal. Clinical Microbiology and Infection. 14 (Suppl. 7): S483 (Abstr. 1665). 23. Freitas AR, Francia MV, Peixe L., Ruíz- Garbajosa P, Novais C, Zervos M, Baquero F, Coque TM Plasmid Characterization of Vancomycin Resistant Enterococcus faecalis Strains from Different Continents ( ). Clinical Microbiology and Infection. 14 (Suppl. 7): S624 (Abstr. 2112). 24. Freitas AR, Francia MV, Peixe L, Novais C, Jensen L, Willems R, Baquero F, Coque TM Plasmid characterization of vancomycin resistant Enterococcus faecium strains from different continents ( ). Clinical Microbiology and Infection. 14 (Suppl. 7): S623 (Abstr. 2111). 25. Novais C, Freitas AR, Moreira L, Francia MV, Baquero F, Peixe L., Coque T.M Characterization of Gram Positive Conjugative Transposons in Enterococci from different sources by a Multiplex- PCR assay. Clinical Microbiology and Infection. 14 (Suppl 7): S331 (Abstr. 1190). 26. Freitas A, Novais C, Coque TM, Peixe L Interhospital dissemination of glycopeptide resistance among enterococcal clinical isolates from Portugal is associated to spread of epidemic pmg1- like plasmids carrying diverse Tn1546 variants. Clinical Microbiology and Infection. 13 (Suppl. 1): S165 (Abstr. 688). 27. Novais C, Freitas A, Coque TM, Peixe L Multi- drug resistant enterococci among Portuguese swine after growth promoter ban. Clinical Microbiology and Infection. 13 (Suppl. 1): S6 (Abstr. O24). 5

6 5. Nucleotide sequences published in GenBank Nucleotide sequence accession numbers HM HM565236, HM HM043744, and JN JN Participation in scientific projects 1. INTERNATIONAL - Funded by the European Union / Spain 1. ESCMID Research Grant Identification of the risk factors promoting the emergence of Enterococcus faecium at the interphase between humans, foodborne animals and the environment ( ). Partners: REQUIMTE- Microbiology, Faculty of Pharmacy, University of Porto; Hospital Universitario Ramón y Cajal, Madrid, Spain; Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto. Financiamento: European Society of Clinical Microbiology and Diseases (ESCMID). Type of participation: Principal Investigator (PI) 2. Evolution and Transfer of Antibiotic Resistance (EVOTAR) Referência: (Call HEALTH ). Financiado pela União Europeia - 7.º Programa Quadro. Rob Willems (Coordenador do projecto, Utrecht, Holanda). Fernando Baquero (Coordenador do grupo, Madrid, Espanha). Parceiros: University Medical Centre Utrecht, Holanda; Wageningen University, Wageningen, Holanda; Instituto Ramón y Cajal de Investigación Sanitaria, Madrid, Espanha; Universidad Complutense de Madrid, Espanha; Agencia Estatal Consejo Superior de Investigaciones Científicas (CSIC), Espanha; University of Copenhagen, Dinamarca; Technical University of Denmark; Biomar, León, Espanha; University of Paris Diderot Medical school, França; The University of Edinburgh, Reino Unido; University of Cantabria, Santander, Espanha; University of Siena, Italy; Institut Pasteur, Paris, França; Microdish, Utrecht/Wageningen, Holanda. Type of participation: Post- Doc Researcher 3. Influencia de los elementos de transferência genética horizontal en la selección y persistência del complejo clonal 17 de Enterococcus faecium: implicaciónes en la selección clonal de patogenos Reference: FIS- PS09/ Financiado por Fondo de Investigaciones Sanitarias, Instituto de Salud Carlos III, Ministerio de Ciencia e Innovacion, Espanha. Type of participation: External consultant 4. Approaches to control multi- resistant enterococci: studies on molecular ecology, horizontal gene transfer, fitness and prevention (ACE)* Reference: ACE- LSHE Financiado pela União Europeia - 6.º Programa Quadro. Rob Willems (Coordenador do projecto, Utrecht, Holanda). Fernando Baquero (Coordenador do grupo, Madrid, Espanha). Partners: University Medical Centre Utrecht, Holanda; Statens Serum Institute, Dinamarca; Danish Technical University, Dinamarca; University of Tromso, Noruega; Norwegian University of Life Sciences, Noruega; Robert Koch Institute, Alemanha; University Medical Center Freiburg, Alemanha; Hospital Ramón y Cajal, Espanha; National Medicines Institute, Polónia; Winclove BV; IQ Corporation BV; Biomade Technology BV. Type of participation: Member of team as PhD student * On behalf of this project, she has the opportunity to present her results in the different scientific mettings organized by the partners of the different countries: 1.1. Freitas AR. The impact of plasmids in the evolution of CC17 Enterococcus faecium. ACE WP2 meeting - Sixth Framework Programme on Research, Technological Development and demonstration de Agosto, Varsóvia, Polónia. 6

7 1.2. Freitas AR. Summary of a detailed plasmid analysis on Enterococcus isolates from hospitals of different countries. ACE WP3/4 meeting - Sixth Framework Programme on Research, Technological Development and demonstration. 1-2 de Dezembro, Copenhaga, Dinamarca Freitas AR. Plasmid characterization of vancomycin resistant Enterococcus faecium and Enterococcus faecium strains causing outbreaks in different countries. ACE Annual meeting - Sixth Framework Programme on Research, Technological Development and demonstration de Abril, Barcelona, Espanha Freitas AR. Characterization of vancomycin- resistant plasmids in clinical enterococci isolates from Portugal. ACE WP3 meeting - Sixth Framework Programme on Research, Technological Development and demonstration de Outubro, Madrid, Espanha. 2. NATIONAL Funded by national agencies 1. Chelators for novel therapeutic strategies. CCDR- N (Comissão de Coordenação da Região Norte) - Quadro de Referência Estratégica Nacional (QREN; ). Referência: NORTE FEDER Type of participation: Researcher 2. Is aquaculture raising more than fish? Detecting antimicrobial resistance in an understudied ecological niche Financiado pela Fundação para a Ciência e Tecnologia (FCT). Referência: PTDC/AAC- AMB/103386/2008. Parceiros: REQUIMTE. Lab. de Microbiologia. Faculdade de Farmácia da Universidade do Porto; Lab. Bromatologia. Faculdade de Farmácia da Universidade de Coimbra. Responsável pelo projecto: Luísa Vieira Peixe. Type of participation: Researcher 3. Selection of antibiotic resistant bacteria: a role for mercury? Projectos Pluridisciplinares Iniciação à Investigação Universidade do Porto/Santander Totta. Parceiros: REQUIMTE- Microbiology, Faculty of Pharmacy, University of Porto; Faculty of Food and Nutrition Sciences, University of Porto, Portugal. Responsável pelo projecto: Carla Novais. 4. Bacterial resistance to copper and antibiotics: two sides of the same problem in animal production setting? Financiado por Projetos Pluridisciplinares Iniciação à Investigação UP/Santander Totta. Parceiros: REQUIMTE. Lab. de Microbiologia. Faculdade de Farmácia da Universidade Porto; Faculdade de Ciências da Nutrição e Alimentação da Universidade do Porto. Responsável pelo projecto: Carla Novais. Type of participation: Member of team as PhD student 5. Population structure of Salmonella nontyphoidal causing human infections by multilocus sequence typing Financiado por Projetos Pluridisciplinares Iniciação à Investigação UP/Santander Totta. Parceiros: REQUIMTE. Lab. de Microbiologia. Faculdade de Farmácia da Universidade Porto; Faculdade de Ciências da Nutrição e Alimentação da Universidade do Porto. Responsável pelo projecto: Luísa Vieira Peixe. Type of participation: Member of team as PhD student 7

8 Scientific meetings 1. Communications (posters/orals) with scientific refereeing 1.1. Oral presentations in international meetings 1. Novais C, Antunes P, Fonte J, Campos J, Silveira E, Grosso F, Machado E, Freitas AR, Silva R, Coque TM, Peixe L Occurrence of antibiotic- resistant enterococci from antibiotic- free trout aquacultures. 22 nd European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). London, UK. 2. Freitas AR, Ruiz- Garbajosa P, Francia MV, Novais C, Zervos MJ, Donabedian S, Peixe L, Baquero F, Coque TM Spread of vancomycin- resistant among Enterococcus faecalis: the influence of pad1 and Inc18- plasmids ( ). 21 th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Milan, Italy. 3. Tedim AP, Freitas AR, Baquero F, Coque TM Fitness cost of widely disseminated conjugative plasmids carrying Tn1546- vana among Enterococcus faecium and Enterococcus faecalis. 21 th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Milan, Italy. 4. Quinteira S, Novais C, Antunes P, Campos J, Freitas AR, Abdukadir A, Monteiro N, Peixe L Tracking antibiotic resistance along the Silk Road. 21 th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Milan, Italy. 5. López M, Tedim AP, Freitas AR, Baquero F, Torres C, Coque TM Diversidad y evolución de Enterococcus faecium- vana ST78 en diferentes países ( ). XV Reunión de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC). Malaga, Spain. 6. Tedim AP, Freitas AR, Baquero F, Coque TM Coste biológico de plásmidos de resistencia a la vancomicina (Tn1546- vana) en Enterococcus faecium y Enterococcus faecalis. XV Reunión de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC). Malaga, Spain. 7. Freitas AR, Ruiz- Garbajosa P, Francia MV, Novais C, Zervos MJ, Donabedian S, Peixe L, Baquero F, Coque TM Influence of pad1 and Inc18- plasmids in the worldwide spread of vancomcycin- resistant Enterococcus faecalis outbreak strains ( ). Comunicação oral S5:3. 3 rd ASM Conference on Enterococci. Portland, Oregon, USA. 8. Freitas AR, Tedim AP, Novais C, Ruiz- Garbajosa P, Cantón R, Peixe L, Baquero F, Coque TM Diseminación global de megaplásmidos portadores de hyl Efm en cepas internacionales de Enterococcus faecium CC17. XIV Reunión de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC). Barcelona, Spain. 9. Coque TM, Ruiz- Garbajosa P, Tedim AP, Freitas AR, Baquero F, Bou G, Campos J, Cantón R, Francia MV, Liñares J, Martínez L, Oteo J, Rodríguez CM, Torres C, Valdezate S, Zamarrón P Estructura poblacional de Enterococcus faecium resistente a vancomicina (EfmRV) en hospitales españoles ( ). XIV Reunión de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC). Barcelona, Spain. 10. Freitas AR, Tedim- Pedrosa A, Novais C, Peixe L, Baquero F, Coque TM Diversity of hyl plasmids among international CC17 Enterococcus faecium strains ( ). ESCMID Conference on Enterococci: from Animals to Man. Barcelona, Spain. 11. Freitas AR, Francia MV, Peixe L, Novais C, Pires J, Tedim- Pedrosa A, Jensen L, Willems R, Baquero F, Coque TM, Peixe L Population structure of international vancomycin resistant Enterococcus faecium (VREfm) strains causing outbreaks. ESCMID Conference on Enterococci: from Animals to Man. Barcelona, Spain. 12. Ruiz- Garbajosa P, Freitas AR, Zervos M, Donabedian S, Cantón R, Baquero F, Coque TM Estructura poblacional y análisis molecular comparativo de Enterococcus faecalis resistente a vancomicina asociados a brotes nosocomiales en Europa y América. XIII Reunión de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC). Madrid, Spain. Award of best oral presentation. 13. Novais C, Freitas AR, Coque TM, Peixe L Multi- drug resistant enterococci among Portuguese swine after growth promoter ban. 17 th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Munich. Germany. 8

9 1.2. Oral presentations in national meetings 1. Novais C, Freitas AR, Coque TM, Nogueira A, Silva R, Dias A, Sousa JC, Peixe L Portuguese piggeries as reservoir of antibiotic multi- resistant enterococci and International spread of vancomycin- resistant Enterococcus faecium (CC17, CC5) and Enterococcus faecalis (CC2) among animals and humans. I Encontro Parcerias para a Investigação. Vairão, Portugal Posters in international meetings 1. Tedim- Pedrosa A, Tobes R, Manrique M, Freitas AR, Peixe L, Baquero F, Coque TM Ecogenetics of antibiotic resistance plasmids of Enterococcus faecium (Efm) and Enterococcus faecalis (Efc). International Plasmid Biology Conference Santander, Spain. 2. Silveira E, Freitas AR, Barros M, Pinho H, Antunes P, Coque TM, Peixe L, Novais C Large conjugative enterococcal plasmids from different sources carry antibiotic resistance genes and a diversity of mera (mercury) genes spread among Gram- positive bacteria. 3rd ASM Conference on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens in Animals, Humans and the Environment. Aix- en- Provence, France. 3. Novais C, Antunes P, Silveira E, Campos J, Fonte J, Grosso F, Machado E, Freitas AR, Coque TM, Peixe L Influx of Enterococcus carrying antibiotic, copper and virulence resistance genes in antibiotic- free trout aquacultures by river water and feed. 3rd ASM Conference on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens in Animals, Humans and the Environment. Aix- en- Provence, France. 4. Silveira E, Freitas AR, Antunes P, Coque TM, Peixe L, Novais C Spread of large conjugative plasmids carrying antibiotic, copper and mercury resistance genes among Enterococcus from different sources. 22 nd European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). London, UK. 5. Freitas AR, Tedim AP, Novais C, Ruiz- Garbajosa P, Peixe L, Baquero F, Coque TM Global dissemination of vancomycin- resistant VanB Enterococcus faecium causing outbreaks in different countries is mainly associated with chromosomal Tn1549/5382- like platforms. 22 nd European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). London, UK. 6. Antunes P, Campos J, Novais C, Machado E, Mourão J, Grosso F, Quinteira S, Freitas AR, Coque TM, Peixe L Aquacultures as reservoirs of pathogenic bacteria and clinically relevant antibiotic resistance genes. 22 nd European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). London, UK. 7. Silveira E, Freitas AR, Coque TM, Peixe L, Novais C Dissemination of two copper resistance genes (tcrb and CueO) in multidrug resistant Enterococci from different sources. 4th FEMS Congress of European Microbiologists. Geneve, Switzerland. 8. López M, Tedim AP, Freitas AR, Baquero F, Torres C, Coque TM Diversity within VanA Enterococcus faecium ST78 (CC17) in different countries ( ). 4th FEMS Congress of European Microbiologists. Geneve, Switzerland. 9. Novais C, Escada R, Freitas AR, Silveira E, Coque TM, Peixe L High incidence of horizontal transfer of ampicillin resistance (pbp5) among CC17 Enterococcus faecium. 21 th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Milan, Italy. 10. López M, Tedim AP, Freitas AR, Baquero F, Torres C, Coque TM Diversidad y evolución de Enterococcus faecium- vana ST78 en diferentes países ( ). XV Reunión de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC). Malaga, Spain. 11. Tedim AP, Freitas AR, Baquero F, Coque TM Coste biológico de plásmidos de resistencia a la vancomicina (Tn1546- vana) en Enterococcus faecium y Enterococcus faecalis. XV Reunión de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC). Malaga, Spain. 12. Werner G, Freitas AR, Coque TM, Sollid JE, Lester C, Hammerum AM, Garcia- Migura L, Jensen LB, Francia MV, Witte W, Willems RJ, Sundsfjord A Transferability of enterococcal vana plasmids among Gram- positive, intestinal bacteria. International Conference on Antimicrobial Research (ICAR). Valladolid, Spain. 9

10 13. Antunes P, Mourão J, Freitas AR, Peixe L Molecular characterization of multidrug- resistant nontyphoidal Salmonella enterica isolates from Portugal. 9 th International Meeting on Microbial Epidemiological Markers (IMMEM). Wernigerode, Germany. 14. Tedim AP, Miranda AC, Ruiz- Garbajosa P, Freitas AR, Francia MV, Baquero F, Coque TM Plasmid diversity among Enterococcus faecalis clinical isolates from Spain ( ). Poster 73A. 3 rd ASM Conference on Enterococci. Portland, Oregon, USA. 15. Freitas AR, Ruiz- Garbajosa P, Francia MV, Novais C, Zervos MJ, Donabedian S, Peixe L, Baquero F, Coque TM Influence of pad1 and Inc18- plasmids in the worldwide spread of vancomcycin- resistant Enterococcus faecalis outbreak strains ( ). Poster 54B. 3 rd ASM Conference on Enterococci. Portland, Oregon, USA. 16. Freitas AR, Tedim AP, Francia MV, Jensen LB, Novais C, Ruiz- Garbajosa P, Willems R, Peixe L, Baquero F, Coque TM Plasmid diversity among Enterococcus faecium belonging to the polyclonal sub- cluster CC17: their impact in the spread of vancomycin resistance worldwide ( ). Poster 72B. 3 rd ASM Conference on Enterococci. Portland, Oregon, USA. 17. Novais C, Freitas AR, Baquero F, Peixe L, Roberts AP, Coque TM Spread of tets among enterococci is associated with CTn6000 and plasmids. Poster 61A. 3 rd ASM Conference on Enterococci. Portland, Oregon, USA. 18. Novais C, Freitas AR, Francia MV, Peixe L, Baquero F, Coque TM Distribution of Gram Positive Conjugative Transposons among vancomycin- resistant Enterococcus faecalis and Enterococcus faecium associated with international hospital outbreaks. Poster 71A. 3 rd ASM Conference on Enterococci. Portland, Oregon, USA. 19. Novais C, Freitas AR, Baquero F, Peixe L, Coque TM Enterococci from human, animal and environmental sources are reservoir of Gram Positive conjugative transposons. Poster 70B. 3 rd ASM Conference on Enterococci. Portland, Oregon, USA. 20. Freitas AR, Tedim AP, Novais C, Ruiz- Garbajosa P, Cantón R, Peixe L, Baquero F, Coque TM Diseminación global de megaplásmidos portadores de hyl Efm en cepas internacionales de Enterococcus faecium CC17. Poster 531. XIV Reunión de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC). Barcelona, Spain. 21. Coque TM, Ruiz- Garbajosa P, Tedim AP, Freitas AR, Baquero F, Bou G, Campos J, Cantón R, Francia MV, Liñares J, Martínez L, Oteo J, Rodríguez CM, Torres C, Valdezate S, Zamarrón P Estructura poblacional de Enterococcus faecium resistente a vancomicina (EfmRV) en hospitales españoles ( ). Poster 532. XIV Reunión de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC). Barcelona, Spain. 22. Novais C, Freitas AR, Baquero F, Peixe L, Roberts AP, Coque TM Diversity of CTn6000 among enterococci from different ecological niches. Poster th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Vienna, Austria. 23. Silveira E, Freitas AR, Coque TM, Peixe L, Novais C Co- dispersion of copper and antibiotic resistance genes among enterococci from different ecological niches, Portugal. Poster th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Vienna, Austria. 24. Antunes P, Mourão J, Freitas AR, Peixe L Population structure of multidrug- resistant non- typhoidal Salmonella enterica isolates from Portugal. Poster th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Vienna, Austria. 25. Freitas AR, Tedim- Pedrosa A, Novais C, Peixe L, Baquero F, Coque TM Diversity of hyl plasmids among international CC17 Enterococcus faecium strains, Poster th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Vienna, Austria. 26. Freitas AR, Novais C, Cunha A, Silveira E, Peixe L, Coque TM Plasmid analysis of vancomycin- resistant enterococci isolates from Portuguese poultry and healthy humans. Poster th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Vienna, Austria. 27. Freitas AR, Tedim- Pedrosa A, Novais C, Peixe L, Baquero F, Coque TM Diversity of hyl plasmids among international CC17 Enterococcus faecium strains ( ). ESCMID Conference on Enterococci: from Animals to Man. Barcelona, Spain. 28. Freitas AR, Francia MV, Peixe L, Novais C, Pires J, Tedim- Pedrosa A, Jensen L, Willems R, Baquero F, Coque TM, Peixe L Population structure of international vancomycin resistant Enterococcus faecium (VREfm) strains causing outbreaks. ESCMID Conference on Enterococci: from Animals to Man. Barcelona, Spain. 10

11 29. Tedim- Pedrosa A, Freitas AR, Ruiz- Garbajosa P, Francia MV, Baquero F, Coque TM Plasmid characterization of ampicillin resistant and ampicillin susceptible Enterococcus faecium causing bacteraemia in a Spanish University hospital in the last 14 years ( ). ESCMID Conference on Enterococci: from Animals to Man. Barcelona, Spain. 30. Novais C, Freitas AR, Silveira E, Baquero F, Peixe L, Coque TM Distribution of Gram positive conjugative transposons among enterococci from human, animal and environmental sources. ESCMID Conference on Enterococci: from Animals to Man. Barcelona, Spain. 31. Gomes F, Silva R, Freitas AR, Sousa JC, Coque TM, Peixe L, Novais C Co- selection of copper and antibiotic resistance genes in enterococci from piggeries, Portugal. 3 rd FEMS Congress of European Microbiologists. Gothenburg, Sweden. 32. Branquinho F, Freitas AR, Soares L, Amorim M, Gião M, Pereira C, Pintado M, Peixe L Genomic diversity and relationship of Bacillus pumilus isolates by PFGE fingerprinting. 3 rd FEMS Congress of European Microbiologists. Gothenburg, Sweden. 33. Freitas AR, Novais C, Silva RM, Coque TM, Peixe L International spread of of vancomycin- resistant Enterococcus faecium (CC17, CC5) and Enterococcus faecalis CC2 among animals and humans. Poster th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Helsinki, Finland. 34. Freitas AR, Novais C, Francia MV, Peixe L, Coque TM Plasmid analysis of vancomycin- resistant Enterococcus faecium isolates from hospitals and aquatic environments in Portugal ( ). Poster th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Helsinki, Finland. 35. Novais C, Freitas AR, Barros C, Baquero F, Peixe L, Coque TM Portuguese healthy human volunteers are reservoirs of conjugative transposons associated with tetracycline and/or erythromycin resistance. Poster th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Helsinki, Finland. 36. Novais C, Freitas AR, Baquero F, Peixe L, Coque TM Spread of EfcTn1 in enterococci from different ecological niches. Poster th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Helsinki, Finland. 37. Novais C, Freitas AR, Francia MV, Peixe L, Baquero F, Coque TM Characterization of Gram- positive conjugative transposons among international epidemic vancomycin- resistant isolates of Enterococcus faecalis and Enterococcus faecium. Poster th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Helsinki, Finland. 38. Tedim AP, Freitas AR, Ruiz- Garbajosa P, Baquero F, Coque TM Plasmid analysis of ampicillin- resistant and ampicillin- susceptible Enterococcus faecium causing bacteraemia in a Spanish university hospital ( ). Poster th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Helsinki, Finland. 39. Freitas AR, Novais C., Ruiz- Garbajosa P., Francia M.V., Silva R.M., Baquero F., Peixe L., Coque T.M Genetic Dynamics of a Successful Enterococcus faecalis Clone Persistently Recovered from European Hospitals during the Last Decade ( ). 48 th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC). Washington, USA. 40. Freitas AR, Francia MV, Peixe L, Novais C, Jensen L, Willems R, Baquero F, Coque TM Plasmid analysis of international vancomycin resistant Enterococcus faecium strains causing outbreaks in the last 20 years ( ). International Plasmid Biology Conference (IPBC). Gdansk, Poland. 41. Freitas AR, Francia MV, Peixe L, Ruiz- Garbajosa P, Novais C, Baquero F, Coque TM Plasmid analysis of international vancomycin resistant Enterococcus faecalis strains ( ). International Plasmid Biology Conference (IPBC). Gdansk, Poland. 42. Novais C, Freitas AR, Coque TM, Nogueira A, Silva R, Dias A, Sousa JC, Peixe L Portuguese piggeries as reservoir of antibiotic multi- resistant enterococci. 1 st ASM Conference on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens. Copenhagen, Denmark. 43. Freitas AR, Novais C, Ruíz- Garbajosa P, Coque TM, Peixe L Molecular characterization of Enterococcus isolates from Portugal. 8 th International Meeting on Microbial Epidemiological Markers (IMMEM). Zakopane, Poland. 44. Ruiz- Garbajosa P, Freitas AR, Zervos M, Donabedian S, Cantón R, Baquero F, Coque TM Estructura poblacional y análisis molecular comparativo de Enterococcus faecalis resistente a vancomicina asociados a brotes nosocomiales en Europa y América. XIII Reunión de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC). Madrid, Spain. 11

12 45. Freitas AR, Novais C, Ruíz- Garbajosa P, Coque TM, Peixe L Population Structure of Enterococcus isolates from Portugal. 18 th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Barcelona, Spain. 46. Freitas AR, Francia MV, Peixe L, Ruíz- Garbajosa P, Novais C, Zervos M, Baquero F, Coque TM Plasmid Characterization of Vancomycin Resistant Enterococcus faecalis Strains from Different Continents ( ). 18 th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Barcelona, Spain. 47. Freitas AR, Francia MV, Peixe L, Novais C, Jensen L, Willems R, Baquero F, Coque TM Plasmid characterization of vancomycin resistant Enterococcus faecium strains from different continents ( ). 18 th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Barcelona, Spain. 48. Novais C, Freitas AR, Moreira L, Francia MV, Baquero F, Peixe L, Coque TM Characterization of Gram Positive Conjugative Transposons in Enterococci from different sources by a Multiplex- PCR assay. 18 th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Barcelona, Spain. 49. Freitas AR, Novais C, Coque TM, Peixe L Persistence of vancomycin- resistance in Portuguese poultry setting despite growth ban interventions: Horizontal transfer plays a relevant role? 4 th Congress of the European Society for Emerging Infections. Lisbon, Portugal. 50. Freitas AR, Novais C, Coque TM, Peixe L In Vitro Activity of Tigecycline against enterococci isolates from different sources of Portugal. 47 th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC). Chicago, USA. 51. Freitas AR, Novais C, Coque TM, Peixe L Interhospital dissemination of glycopeptide resistance among enterococcal clinical isolates from Portugal is associated to spread of epidemic pmg1- like plasmids carrying diverse Tn1546 variants. 17 th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Munich, Germany. 52. Freitas AR, Novais C, Coque TM, Peixe L Large conjugative plasmids associated to the spread and persistence of vancomycin resistant enterococci in Portuguese hospitals. 2 nd FEMS Congress of European Microbiologists. Madrid. Spain Posters in national meetings 1. Mendes M, Novais C, Antunes P, Campos J, Freitas A, Abdukadir A, Peixe L, Monteiro N, Quinteira S Tracking antibiotic resistance along the Silk Road. V Encontro de Jovens Investigadores da Universidade do Porto. Porto, Portugal. 2. Dias L, Silveira E, Antunes P, Freitas A, Peixe L, Novais C. Bacterial resistance to copper and antibiotics: two sides of the same problem in the animal production setting? V Encontro de Jovens Investigadores da Universidade do Porto. Porto, Portugal. 3. Silveira E, Freitas AR, Antunes P, Coque TM, Peixe L, Novais C Spread of large plasmids carrying antibiotic and copper resistance genes among enterococci from different ecological niches. Congresso Nacional da Sociedade Portuguesa de Microbiologia e Sociedade Portuguesa de Biotecnologia (MicroBiotec 11). Braga, Portugal. 4. Silveira E, Freitas AR, Antunes P, Coque TM, Peixe L, Novais C Estará o uso de cobre a contribuir para a selecção de bactérias resistentes aos antibióticos na produção animal e em humanos? 4ª Reunião Anual PortFIR - Gestão da Informação para a Nutrição e Segurança Alimentar. Lisbon, Portugal. 5. Mourão J, Pires J, Nunes H, Silva A, Costa C, Freitas AR, Peixe L, Antunes P Population structure of nontyphoidal Salmonella causing human infections by Multi- Locus Sequence Typing. IV Encontro de Jovens Investigadores da Universidade do Porto. Porto, Portugal. 6. Branquinho FR, Amorim MM, Freitas AR, Pintado MM, Peixe L PFGE typing methodology and proteomic analysis for assessing Bacillus pumilus diversity. Congresso Nacional da Sociedade Portuguesa de Microbiologia e Sociedade Portuguesa de Biotecnologia (MicroBiotec 09). Vilamoura, Portugal. 7. Freitas AR, Novais C, Coque TM, Peixe L International spread of vancomycin- resistant Enterococcus faecium (CC17, CC5) and Enterococcus faecalis (CC2) among animals and humans. I Encontro Parcerias para a Investigação. Vairão, Portugal. 12

13 8. Silva RM, Freitas AR, Novais C, Coque TM, Peixe L Population structure of Enterococcus isolates from Portuguese hospitals ( ). II Encontro de Jovens Investigadores da Universidade do Porto. Porto, Portugal. 9. Freitas AR, Novais C, Coque TM, Peixe L Vancomycin- resistant enterococci widely spread in Portuguese hospitals are associated with vana plasmids carrying diverse Tn1546 variants. Poster. Congresso Nacional da Sociedade Portuguesa de Microbiologia e Sociedade Portuguesa de Biotecnologia and XXXIII Jornadas Portuguesas de Genética (Micro 07- Biotec 07- XXXIII JPG). Lisbon, Portugal. 10. Novais C, Freitas AR, Coque TM, Sousa JC, Peixe L Contribution of Portuguese piggeries to dissemination of multi- drug resistant enterococci in environment. Poster. Congresso Nacional da Sociedade Portuguesa de Microbiologia e Sociedade Portuguesa de Biotecnologia and XXXIII Jornadas Portuguesas de Genética (Micro 07- Biotec 07- XXXIII JPG). Lisbon, Portugal. 11. Freitas AR, Coque TM, Peixe L Ecology and evolution of local genetic elements related with antimicrobial resistance in Enterococcus: study of the factors responsible for stability and dissemination of multiresistant strains in distinct metagenomes. Encontro REQUIMTE Rede de Química e Tecnologia. Fatima, Portugal. 2. Attendance to congresses/symposium/scientific meetings th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Organizado por: European Society of Clinical Microbiology and Infectious Diseases (ESCMID) April Vienna, Austria. 2. Biofilmes bacterianos Mecanismos de formação, prevenção e tratamento. 5 May Doctoral Programme of Faculty of Pharmacy, University of Porto, Portugal. 3. Clones, genes e elementos genéticos móveis: peças do puzzle da resistência a antibóticos. In: Clones, genes e elementos genéticos móveis: peças do puzzle da resistência a antibióticos March Doctoral Programme of Faculty of Pharmacy, University of Porto, Portugal. 4. Final Meeting of the European Scientific Project ACE - Sixth Framework Programme on Research, Technological Development and demonstration January Amsterdam, The Netherlands. 5. V Congresso Cientifico Antibioterapia: uma abordagem complementar" November Associação de Estudantes da Faculdade de Farmácia do Porto, Portugal. 6. Microbiome: deciphering the last organ of human body. 18 th Scientific Symposium organized by Fundación Lilly November Madrid, Spain. 7. ESCMID Conference on Enterococci: from Animals to Man. European Society of Clinical Microbiology and Infectious Diseases (ESCMID) November Barcelona, Spain. 8. I Reunión de la Red Española de Elementos Extracromosómicos (REDEEX). Spanish Network for the Study of Plasmids and Extrachromosomal Elements (REDEEX) September Las Navas del Marqués, Ávila, Spain. 9. WP2 Meeting of the European Scientific Project ACE - Sixth Framework Programme on Research, Technological Development and demonstration August Warsaw, Poland th ESCMID Summer School. European Society of Clinical Microbiology and Infectious Diseases (ESCMID) July Porto, Portugal th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Organizado por: European Society of Clinical Microbiology and Infectious Diseases (ESCMID) May Helsinki, Finland. 12. WP3/4 Meeting of the European Scientific Project ACE - Sixth Framework Programme on Research, Technological Development and demonstration. 1-2 December Copenhagen, Denmark th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC). American Society for Microbiology (ASM) October Washington, USA 14. Bacterial Identification, Typing and Detection (Workshop). Instituto de Biologia Molecular e Celular (IBMC). 30 June Porto, Portugal. 13

14 15. 8 th International Meeting on Microbial Epidemiological Markers (IMMEM). Co- patrocinado por: American Society for Microbiology (ASM) e European Society of Clinical Microbiology and Infectious Diseases (ESCMID) May Zakopane, Poland. 16. Annual Meeting of the European Scientific Project ACE - Sixth Framework Programme on Research, Technological Development and demonstration April Barcelona, Spain st European Day of Fighting Infection. European Society of Clinical Microbiology and Infectious Diseases (ESCMID). 23 April Barcelona, Spain th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Organizado por: European Society of Clinical Microbiology and Infectious Diseases (ESCMID) April Barcelona, Spain. 19. Environmental changes, microbial systems and infections. 12 th Scientific Symposium organized by Fundación Lilly November Madrid, Spain. 20. WP3 Meeting of the European Scientific Project ACE - Sixth Framework Programme on Research, Technological Development and demonstration. October Madrid, Spain th Congress of the European Society for Emerging Infections. 30 Setember - 3 October Lisbon, Portugal th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC). American Society for Microbiology (ASM) September Chicago, USA. 23. First Meeting of the European Scientific Project ACE - Sixth Framework Programme on Research, Technological Development and demonstration. April Tromso, Norway th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Organizado por: European Society of Clinical Microbiology and Infectious Diseases (ESCMID). 31 March - 3 April Munich, Germany nd FEMS Congress of European Microbiologists. Federation of European Microbiological Societies. 4-8 July Madrid, Spain. 26. Course of Antibiotherapy (18 hours). Department of Microbiology, Faculty of Health Sciences, University Fernando Pessoa. May Porto, Portugal. 27. Seminary of Biological Safety. 28 April Faculty of Medicine, University of Coimbra, Portugal º Encontro da Rede de Química e Tecnologia REQUIMTE Fátima, Portugal. 3. Comunications as invited speaker 1. Freitas AR. O impacto da transferência genética horizontal em Enterococcus In: Clones, genes e elementos genéticos móveis: peças do puzzle da resistência a antibióticos. Doctoral Programme of Faculty of Pharmacy, University of Porto, Portugal. 2. Freitas AR. Enterococcus spp.: comensal ou patogénico? II Edição de Seminários em Microbiologia do Curso de Mestrado em Microbiologia Clínica. Faculty of Health Sciences, University Fernando Pessoa. Porto, Portugal. 4. Events organization Local organization and secretariat support of the 8th ESCMID Summer School held in Porto, Portugal during July

15 Experience as scientific adviser * Scientific initiation in undergraduate students at Faculty of Pharmacy, University of Porto on behalf of the project Scientific Research in Pre- graduation : - Ana Isabel Cunha. Caracterização molecular de Enterococcus spp. resistentes à vancomicina de origem animal: haverá identidade com os isolados obtidos de humanos? Graduate Thesis, Estrutura populacional de Enterococcus spp. resistences à vancomicina de origem animal, Rosa Maria Silva. Caracterização molecular de isolados de Enterococcus spp. de hospitais Portugueses: estrutura populacional e diversidade de plasmídeos Rosa Alves Correia. Actividade in vitro da tigeciclina em Enterococcus spp. de diferentes origens Márcia Monteiro. Actividade in vitro da tigeciclina em Enterococcus spp. de diferentes origens João Pires. Characterização de plasmídeos em Enterococcus faecium resistentes à vancomicina de hospitais Portugueses ( ) * Supervisor of the Pharmacetutical Sciences licenciate degree conclusion work at Faculty of Pharmacy, University of Porto - Vanessa Azevedo Insuficiência Venosa: da sensação de pernas cansadas à patologia crónica. - Sofia Mateus Dermatite Atópica. Other merits 1. Travel grant attributed by the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) for assistance of the 22 nd European Congress of Clinical Microbiology and Infectious Diseases. March- April London, UK. 2. Attendance grant attributed by the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) for assistance of the ESCMID Conference on Enterococci: from animals to man. November Barcelona, Spain. 3. Travel grant attributed by the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) for assistance of the 19 th European Congress of Clinical Microbiology and Infectious Diseases. May Helsinki, Finland. 4. Travel grant attributed by the American Society of Microbiology (ASM) for assistance of the 48 th Interscience Conference on Antimicrobial Agents and Chemotherapy. September Washington, USA. 15

16 5. Award for the best scientific oral communication attributed by the Sociedad Española de Enfermedades Infeccionas y Microbiología Clínica (SEIMC) in the XIII Congreso de la Sociedad Española de de Enfermedades Infeccionas y Microbiología Clínica. May Madrid, Spain. 6. Attendance grant attributed by the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) for assistance of the 8 th International Meeting on Microbial Epidemiological Markers. May Zakopane, Poland. 7. Travel grant attributed by the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) for assistance of the 18 th European Congress of Clinical Microbiology and Infectious Diseases. April Barcelona, Spain. 8. Travel grant attributed by the American Society of Microbiology (ASM) for assistance of the 47 th Interscience Conference on Antimicrobial Agents and Chemotherapy. September Chicago, USA. Other activities Reviewer for Journal of Antimicrobial Chemotherapy Microbial Drug Resistance Scandinavian Journal of Infectious Diseases Journal of Global Antimicrobial Resistance Journal of Infection in developing countries 16