PREPARING DIGITAL IMAGES FOR PUBLICATION

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1 Introductory and Intermediate Users Workshop PREPARING DIGITAL IMAGES FOR PUBLICATION UCLA Molecular Biology Interdepartmental Graduate Program May 14, 2008 Michael Hepp Technology Strategist The Sheridan Group Journals

2 Topics General Submission Guidelines for the following journals: Nature Publishing Group Cell Press Genetics Journal of Cell Biology Journal of Experimental Medicine Journal of General Physiology Journal of Lipid Research Biophysical Journal The Plant Cell Plant Physiology American Journal of Botany Journal of Neuroscience 2

3 Topics Imaging Foundations RGB Image Submission and Color Management A Few Helpful Tips 3

4 Vector Images 4

5 Vector Images Vector graphics are made up of lines and curves called vectors. Created in drawing programs such as Illustrator, Freehand, CorelDraw, Word, PowerPoint. Vector graphics are resolution-independent Fonts need to embedded or converted to outlines or paths. Lines minimum of.25 pts. 5

6 Raster Images 6

7 Raster Images Raster images are made up of pixels. Created by capture devices such as digital cameras or processed in image editing programs such as Photoshop. Raster images are resolution dependent. Text and lines are raster elements and are not editable 7

8 Resolution 72 ppi 300 ppi 8

9 Effective Resolution Actual resolution factored for scaling 9

10 Printing Process Halftone Screening 10

11 Printing Process Halftone Screening 11

12 Types of Images Line Art Halftones Combinations 12

13 Line Art Images (Bitmap) Images comprised of black and white elements only 1000 ppi minimum resolution 1200 ppi preferred for thin line reproduction 13

14 Line Art Images (Bitmap) 14

15 Line Art Images (Bitmap) 1000 ppi 600 ppi 300 ppi 15

16 Halftone Image only Grayscale or color 300 ppi minimum resolution 16

17 Halftone 17

18 Combination (Combo) Halftone and line art combined Grayscale or color 600 ppi minimum resolution ppi maximum 18

19 Combination (Combo) 19

20 File formats TIFF Tagged Image File Format EPS Encapsulated PostScript JPEG Joint Photographic Experts Group GIF Graphics Interchange Format PDF Portable Document Format MS OFFICE DOC, PPT, XLS 20

21 Recommendations TIFF EPS Also accetpable, if prepared correctly: JPEG (compression) PDF (Distiller Settings) *Microsoft Office (Guidelines and settings) 21

22 Fonts and font embedding Use standard font families when labeling images and be consistent throughout your images Helvetica or Ariel Times Roman Symbol Mathematical Pi European Pi 22

23 Font embedding When submitting vector files either embed the fonts (when possible) or convert to outlines 23

24 Font embedding Adobe Illustrator 24

25 Font embedding Microsoft PowerPoint 25

26 File compression Lossless Compression LZW, ZIP, CCITT G4 Lossy Compression JPEG 26

27 File compression Lossless techniques JPEG compression 27

28 Color Mode Author Supplied CMYK Author Supplied RGB Sheridan Converted CMYK 28

29 RGB Submission Most journals requires the submission of images in RGB color mode Easier for authors Color managed conversion to CMYK Opportunity for full gamut RGB online 29

30 ICC Profiles Describe a devices ability to render color Necessary for accurate conversion to CMYK 30

31 ICC profiles Adobe Ricoh 31

32 Embedding ICC profiles Adobe Photoshop Adobe Illustrator 32

33 Preparation Tips 33

34 Find balance of quality/size IMAGE SIZE: 3 x ppi RGB EPS 24.7MB RGB TIFF with NO compression 8.9MB RGB TIFF with LZW 3.3MB RGB JPEG, Quality KB IMAGE SIZE: 3 x ppi RGB TIFF with LZW 1.6MB 34

35 Print quality digital photos Choose settings that maximize quality JPEG, TIFF, and RAW formats JPEG quality settings = compression Balance quality with file size RAW format is the new digital negative Capture in RAW, convert to TIFF 35

36 Alternate applications Adobe Photoshop Elements Web-based Image Editors (Picnik, PS Express) Open Source applications (Gimp) *Just be aware of lack of color management support 36

37 Screen Captures 37

38 Screen Recording 38

39 Avoid Pattern Fills Do not use pattern or textured fills in graphics. Use solid fills or percentage screens. A 20% difference in percent screens is most effective for differentiation. Extremity Mean Image Intensity as a Percentage of Bladder Mean Intensity Percentage Intensity as Part of the Bladder Intensity CJ2R 2RC003 Monkeys Average Control Monkeys Pre 3 Month 6 Month 14 Month 39

40 Crop Excess White Space Use Adobe Photoshop s Trim Feature 40

41 Online Preflight 41

42 Presentation available at: Site: ftp.dartmouthjournals.com Username: digexp Password: 619exp 42

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