InFlux - software parameters and fluorochromes

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1 InFlux - software parameters and fluorochromes 642nm Red & Sortware Parameter Name 530/40 FITC, Alexa 488, egfp, CFSE, Dye Cycle Green, YFP 670/30 PE-Cy5 (also tricolour or cychrome ), 7AAD 710/50 PerCP-Cy5.5, DRAQ5, Dye Cycle Ruby Alexa 405, CFP, Pacific Blue, DAPI, Hoechst (blue), Celltrace Violet, Dye Cycle 460/50 Violet, BV421, VPD450, Calcein Violet 450, Fixable Viability Dye efluor 450, Cell Proliferation Dye efluor /35 Qdot 525, BV510, BD Horizon V LP Qdot655, BV650, Hoechst Red 585/29 PE, Alexa Fluor /20 PE-Texas Red, M-Cherry, M-RFP, Mars, PI 670/30 PE-Cy5 (also t (although aricolour or cychrome ), 7AAD, E2-Crimson, M- Plum 710/50 PE-Cy5.5, DRAQ7, AF LP PE-Cy7, DRAQ7 670/30 APC, Alexa 633, Alexa 647, TO-PRO-3, Cy5, DRAQ5, DRAQ7, Dye Cycle Ruby 720/40 APC-Cy5.5, DRAQ7, Dye Cycle Ruby, AF LP APC-Cy7, APC-H7, DRAQ7, Alexa Fluor 750 Filter modification in the Influx is difficult, consult staff if you need anything not listed here.

2 Aria FUSION - software parameters and fluorochromes Diva Parameter Name 530/30 530/30 blue FITC, Alexa 488, egfp, CFSE, Dye Cycle Green, YFP 695/40 695/40 blue PerCP, PerCP-Cy5.5, PerCP-eFluor710, DRAQ5, Dye Cycle Ruby 670/30 670/30 red APC, Alexa 633, Alexa 647, TO-PRO-3, Cy5, DRAQ5, DRAQ7, Dye Cycle Ruby 730/45 730/45 red APC-Cy5.5, Alexa 700, DRAQ7, Dye Cycle Ruby 780/60 780/60 red APC-Cy7, DRAQ7, Alexa Fluor 750 Alexa 405, CFP, Pacific Blue, DAPI, Hoechst (blue), 450/50 450/50 violet Celltrace Violet, Dye Cycle Violet, BV421, VPD450, Calcein Violet 450, Fixable Viability Dye efluor 450, Cell Proliferation Dye efluor /50 525/50 violet Qdot 525, BV510, BD Horizon V /20 610/20 violet Qdot 605, BV605, efluor605nc 660/20 660/20 violet Qdot 655, BV /50 710/50 violet BV /60 780/60 violet BV /15 582/15 yellow PE, Alexa Fluor /20 610/20 yellow PE-Texas Red, M-Cherry, M-RFP, Mars, PI 670/14 670/14 yellow PE-Cy5 (also tricolour or cychrome ), 7AAD, E2- Crimson, M-Plum 710/50 710/50 yellow PE-Cy5.5, DRAQ7, AF /60 780/60 yellow PE-Cy7, DRAQ7 Filters may be modified; we do have a stock of other filters available. Consult staff if your fluorochrome is not listed.

3 Aria III - software parameters and fluorochromes Diva Parameter Name 530/30 530/30 blue FITC, Alexa 488, egfp, CFSE, Dye Cycle Green, YFP 695/40 695/40 blue PerCP, PerCP-Cy5.5, PerCP-eFluor710, DRAQ5, Dye Cycle Ruby 582/15 582/15 yellow PE, Alexa Fluor /20 610/20 yellow PI, PE-Texas Red, M-Cherry, M-RFP, Mars 670/14 670/14 yellow PE-Cy5 (also tricolour or cychrome ), 7AAD, E2-Crimson, M-Plum 710/50 710/50 yellow PE-Cy5.5, DRAQ7, PE- AF /60 780/60 yellow PE-Cy7, DRAQ7 660/20 660/20 red APC, Alexa 633, Alexa 647, TO-PRO-3, Cy5, DRAQ5, DRAQ7, Dye Cycle Ruby 730/45 730/45 red APC-Cy5.5, DRAQ7, Dye Cycle Ruby, AF /60 780/60 red APC-Cy7, Alexa Fluor 750, DRAQ7, APC- H7 Alexa 405, CFP, Pacific Blue, DAPI, Hoechst (blue), Celltrace 450/50 450/50 violet Violet, Dye Cycle Violet, BV421, VPD450, Calcein Violet 450, Fixable Viability Dye efluor 450, Cell Proliferation Dye efluor 450, VioBlue, Horizon V /50 510/50 violet Qdot 525, BV510, BD Horizon V500, Fixable Viability Dye efluor 506, VioGreen, AmCyan 610/20 610/20 violet BV605, efluor 605nc, Qdot605 Filters may be modified; we do have a stock of other filters available. Consult staff if your fluorochrome is not listed.

4 AVALON - software parameters and fluorochromes ProSort name 520/35 FL1 FITC, Alexa 488, egfp, CFSE, Dye Cycle Green, YFP 575/15 FL2 PE, RFP 615/20 FL3 PI, PE-TxRed, mcherry 650LP FL4 PECy5, PECy7, PerCP, PerCPCy5.5, APC Filters may be modified; we do have a stock of other filters available. s on the Avalon are co-linear so certain combinations of fluorochromes may not be possible, consult staff if in doubt.

5 MoFlo - software parameters and fluorochromes Summit name 530/40 FL1 FITC, Alexa 488, egfp, CFSE, Dye Cycle Green, YFP 580/30 FL2 PerCP, PerCP-Cy5.5, PerCP-eFluor710, DRAQ5, Dye Cycle Ruby FL3 670/20 FL4 PECy5 787/43 FL5 PECy7 355nm Ultraviolet 660/20 FL6 APC, Alexa 633, Alexa 647, TO-PRO-3, Cy5, DRAQ5, DRAQ7, Dye Cycle Ruby 748LP FL7 APC-Cy7 Alexa 405, CFP, Pacific Blue, DAPI, Hoechst (blue), Celltrace 424/44 FL8 Violet, Dye Cycle Violet, BV421, VPD450, Calcein Violet 450, Fixable Viability Dye efluor 450, Cell Proliferation Dye efluor 450, VioBlue, Horizon V LP FL9 BUV785, Hoechst Red Filters may be modified; we do have a stock of other filters available. The MoFlo is the most configurable of the cytometers in the FACS Lab. Filters and numbers of detectors per laser are able to be modified, consult staff if you need advice.