LDC-CEVA Cevac IBird. April 2015

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1 LDC-CEVA Cevac IBird April 2015

2 CONTENT 1. Presentation of LDC group 2. French Infectious Bronchitis epidemiology 3. Actions 4. Monitoring implementation 5. Results

3 LDC - Overview Brittany 10 sites employees 500 farmers Pays de Loire / Centre / Normandy 35 sites employees farmers Paris 2 sites 250 employees Aquitaine 2 sites 250 employees 100 fermers South-East 13 sites 2600 employees 1000 farmers A Group present in every poultry region in France Production sites in poultry production areas : Brittany, Loire Valley, Normandy, Burgundy, Aquitaine,

4 LDC - The Group Organization Poultry Division Convenience Division International (group Export) Upstream France Slaughterhouses France International Division POLAND SPAIN

5 LDC - Group Organization Eggs Grain Collect Poultry Poultry Direction : Gilles HUTTEPAIN

6 LDC Group organization The Products Upstream Division Turnover 610 M Number of farmers Surface m² Production of poultry In front of the Group needs 70 % Chicken 15 % Label rouge chicken 60 % Turkey 50 % Duck 40 % Guinea fowl 6 Tons Feed /year Poultry T From which processed T Collect Cereals/year T Eggs/boxes/year 325 millions eggs Label Rouge, Free range, & Organic 150 millions eggs Standards 475 millions eggs From which 50% under brands Loué & Le Gaulois

7 Direction : Denis LAMBERT LDC - The Group Organization LDC Sablé+ CEPA Cavol

8 LDC - The group Organization The Products Poultry division France Turnover : 2,1 Bn Volume : T Mix Product Poultry /week Mix customers Retail / HD 67 % Prof & Indust 25 % EXPORT 8 % Standard chickens Guinea fowls Label chickens + Org Rabbits Certified chickens Quails Turkeys Squabs Ducks Further-processed products T per week

9 LDC - Our Strategy : Innovations and Brands for a strong organic growth Poultry and convenience : synergy and complementarity in offers Convenience Further processed Cut ups Red Label Poultry Poultry

10 French Infectious bronchitis epidemiology 830 million broilers 43 million layers 45 millions turkeys 120 million ducks.multi species production Infectious bronchitis viruses are circulating. % of broiler production per department Need for a good viral protection

11 French Infectious bronchitis epidemiology Vaccination with Mass vaccine is done on 100 % of the birds, at the hatcheries. Field boosting with either 793 B or QX are applied according to veterinarian, integration or farmer decision related to IB perceived pressure or type of broilers produced (big birds). D0 D10-D14 D16-D20 IB Mass Strain (Hatchery) IB Variant Strain (Field vaccination) 793B/QX GUMBORO

12 French Infectious bronchitis epidemiology => Vaccination at the hatchery is not perceived as critical by the independant hatcheries => Field vaccination is usually applied by spray with no regular monitoring. Need for LDC -to get better control of vaccine & protection on the production. -to get better evaluation of epidemiological situation in real time 12

13 Actions : a new vaccination program Big birds Certified birds D0 D16-D20 IB Mass Strain + IB Variant strain (Cevac IBird). Hatchery GUMBORO HATCHERY IB Vaccination. Monitoring Implementation

14 Actions 230 different poultry houses In 2 regions from nov 14. Vaccination Mass & Cevac IBird spray day 1 7 different hatcheries Control of spray equipment & CHICK Program implementation 3 different organisations Monitoring of day 10 PVR & respiratory signs during growing period 3 different slaughterhouses

15 Monitoring implementation 230 different poultry houses In 2 regions from nov 14. Vaccination Mass & Cevac IBird spray day 1 7 different hatcheries Control of spray equipment & CHICK Program implementation 5 different organisations 3 different slaughterhouses Monitoring of day 10 PVR & respiratory signs during growing period Sampling & Analysis for epidemiological monitoring 10 Blood samples per flock : Biocheck Elisa Cecal tonsils: frozen and PCR analysis according to Elisa & farm results 15

16 Desvac In-Line sprayer Hatchery spray evaluation Desvac In-Line sprayer

17 Results : Safety 1 out of 230 flocks with mortality before 10 days, after veterinarian visit it was due to E coli infection 17

18 Results : IB ELISA MEAN TITERS

19 Results : IBV mapping October-December 2014, 28% of the farms are challenged by a field virus (T> 3000) No vaccination< < «normal»< 3000 Doubtfull > 3000, high CV Field infection > 3000, low CV

20 No vaccination< < «normal»< 3000 Doubtfull > 3000, high CV Field infection > 3000, low CV Results : IBV mapping December 14 February 15, 38% of the farms are challenged by a field virus (T> 3000)

21 Results : Some respiratory signs after 10 days in 9 out of 230 flocks : 1 IB titers OK 1 positive for ORT 1 positive for MS 2 IB PCR >0 no identification possible 4 IB PCR >0 for IBird, no conclusion other cause? 21

22 Conclusion Hatchery vaccination with Mass & Cevac IBird gave consistent seroconversion at slaughter It can be used as hatchery vaccination monitoring Hatchery spray application was improved with equipement setting & control Field virus circulation was detected between 28 % and 38% of the farms according to the period. Reinforced the need of a complete and well applied vaccination program in our production area. 22

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