Comprehensive Examinations for the Program in Bioinformatics and Computational Biology
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1 Comprehensive Examinations for the Program in Bioinformatics and Computational Biology The Comprehensive exams will be given once a year. The format will be six exams. Students must show competency on all six parts and a high degree of competency on four of the six parts. Students will be allowed two attempts to successfully pass the exams. Protein Structure Analysis Complexity of protein structure and protein folding Protein structure verification and validation Secondary structure assignment and prediction Protein structure classification Comparative modeling and fold recognition Protein structure prediction and structural genomics Protein engineering Protein structure databases Suggested primary sources reading at Suggested text: Structural Bioinformatics (Philip Bourne and Helge Weissig, Eds), John Wiley &
2 Sons, Research Methods Descriptive Statistics Probability Discrete Probability Distributions Continuous Probability Distributions Estimation Hypothesis Testing: One- Sample Inference Hypothesis Testing: Two- Sample Inference Multisample Inference Hypothesis Testing: Categorical Data Regression and Correlation Methods Nonparametric Methods Suggested Text: Fundamentals of Biostatistics Bernard Rosner Biological Sequence Analysis Pairwise alignment: Scoring matrixes. Gap penalties. Dynamic programming. Global vs. local alignment: Needleman-Wunsch, Smith-Waterman. Database search algorithms: FASTA, BLAST. Statistical significanse of scores. Markov chains and hidden Markov models: Profile HMMs for sequence families. Deriving profile HMMs from multiple
3 alignments. The HMMER system. Multiple sequence alignment: Scoring functions. Progressive alignment methods. CLUSTALW. Multiple alignment by profile HMM training. Phylogenetic analysis: Distance methods; Parsimony; Maximum likelihood. Gene-finding: Search by content; Search by signals. Suggested Texts: Biological Sequence Analysis by Durbin, Eddy, Krogh and Mitchison (Chapters 1, 2, 3, 5, 6, 7, ). Computational Molecular Biology by Clote and Backhofen Numerical Methods for Bioinformatics Solution of Nonlinear Equations Solution of Simultaneous Linear Algebraic Equations Solution of Matrix Eigenvalue Problem Curve Fitting and Interpolation Random Number Generators Monte Carlo Methods Numerical Differentiation Numerical Integration Ordinary Differential Equations: Initial-Value Problems Ordinary Differential Equations: Boundary-Value Problems Partial Differential Equations Optimization Finite-
4 Element Method Suggested Text: Applied Numerical Methods for Engineers and Scientists By Singiresu S. Rao Systems Biology enzyme-substrate kinetics (Michaelis Menten Kinetics, Law of Mass Action) protein synthesis DNA replication and transcription cellular respiration (glycolysis and oxidative metabolism). cellular ionic homeostasis and excitable membranes (hodgkin huxley model, ion channels, pumps, exchangers). cellular signaling (G-proteins, IP3, Calcium mobilization, ligand receptor dynamics) Suggested text: Molecular Cell Biology, 4th ed; Harvey, Lodish et al. Molecular Biology of the Cell by Bruce Alberts Although there is no specific exam named,
5 students should be knowledgeable in the area of molecular, cellular and biochemistry of the cell since this information is essential for understanding the field of Bioinformatics and Computational Biology. Nucleic acid -chemistry -biochemistry Molecular biology - history of the field - Central Dogma -recent revisions to CD -What is a gene? DNA replication -molecular biology -biochemistry - biotechnology -history of the field Genome sequence analysis - biotechnology -bioinformatics of sequence determination -history of the field -computational resources for analysis Suggested Texts: Molecular Cell Biology, 4th ed; Harvey Lodish et al pdf (Fall 2015 Dr. Aman Ullah)
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