Fantatstic Winter Offers From Roche Applied Science
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1 Fantatstic Winter Offers From Roche Applied Science Come in from the cold this winter and take 50% off the list price of 100 s of products from our extensive range of Enzymes for PCR and Restriction Enzymes. These offers are valid from 1 st October 2008 until 19 th December Roche Applied Science provides an extensive portfolio of Enzymes for PCR to meet all your amplifaction needs. For further details of these products or for help identifying the best products for your project please visit our NEW website at To take advantage of these heart warming offers, Simply send an quoting your contact details, the catalogue numbers and quantities of the items that you require to: Tooran.shahbazi@roche.com A special quotation number will be ed to you the same day. Place your order by telephone, fax, or stating your unique quotation number. FREEphone FREEfax burgesshill.ras@roche.com Terms and Conditions: Offer valid on orders received by telephone, fax or from the 01/10/08 18/12/08. Only orders clearly stating a valid quotation number are eligible to receive discount. Quotation number can not be applied retrospectively to an order. Online orders placed via the Roche Applied Science website are excluded from this promotion. Roche Applied Science may, at its sole discretion, terminate this offer at any time. For Full Terms and Conditions please visit Roche Diagnostics Ltd Charles Avenue Burgess Hill, RH15 9RY Reg:
2 Enzymes, Reagents and Kits for PCR Description Expand Cloning Kit 1 Kit (10 x Rxns, incl control) Human Genomic DNA 100µg Human tpa Control Primer Set 1 Set MgCl 2 Stock Solution 3 x 1ml PCR Buffer Set 2 x 2ml (2 x 1ml of each) PCR Buffer without MgCl 2, 10 x 3 x 1ml PCR Cloning Kit (blunt end) 1 kit (35 x Rxns & contol) PCR Core Kit 1 Kit (100 rxns, 50µl final vol.) PCR Core Kit PLUS rxns, 50µl Kit (50 final vol.) PCR ELISA (DIG Detection) 1 kit PCR ELISA (Dig Detection) 1 Kit (192 detection rxns) PCR ELISA (Dig labeling) 1 Kit (50 rxns) PCR Master 1 Kit (200 PCR rxns, 50µl final vol.) PCR Optimization Kit 1 Kit Pwo Master 2.5ml (10 x 250µl)(100 rxns 50µl final vol.) Rapid DNA Ligation Kit 1 Kit (40 DNA ligation rxns) Uracil-DNA Glycosylase 100U Uracil-DNA Glycosylase (Heatlabile) 100U Uracil-DNA Glycosylase (Heatlabile) 500U Water, PCR Grade 25ml (1x25ml) Water, PCR Grade 100ml (4x25ml) Water, PCR Grade 25ml (25x1ml)
3 Expand 20kb PLUS PCR 200U Expand HiFi PCR 100U Expand HiFi PCR 2,500U Expand HiFi PCR 500U Expand HiFi PCR 100 U Expand HiFi PCR 2,500U Expand HiFi PCR 500U Expand HiFi PLUS PCR 125U Expand HiFi PLUS 2,500U PCR Expand HiFi PLUS 500U PCR Expand HiFi PLUS PCR 125U Expand HiFi PLUS PCR 500U 500U Expand Long Range 175U (50 rxns) ,500U Expand Long Range (5 x 700U) 3500 U (1,000 rxns) Expand Long Range 700U (200 rxns) Expand Long Template PCR 150U Expand Long Template PCR 3,600U (10 x 360U) Expand Long Template PCR 720U (2 x 360U) Expand 20kb PLUS PCR 200U FastStart HiFi PCR 125U FastStart HiFi PCR 500U FastStart High Fidelity PCR 125U FastStart High Fidelity PCR 500U FastStart High Fidelity PCR 2,500U
4 FastStart PCR Master 10ml (8 x 1.25ml)(400 rxns 50µl final vol.) FastStart PCR Master 2ml (2 x 1.25ml)(100 rxns 50µl final vol.) FastStart PCR Master 10 x 5ml (2,000 rxns 50µl final vol.) FastStart Taq DNA Pol., 100U FastStart Taq DNA Polymerase 1,000U, (4 x 250U) FastStart Taq DNA Polymerase 2,500U, FastStart Taq DNA Pol., 500U 500 U FastStart Taq DNA Pol., 5,000U 5000 U (20 x 250U) FastStart Taq DNA Polymerase, 100U FastStart Taq DNA Polymerase, 1,000U (4 x 250U) FastStart Taq DNA Polymerase, 2,500U FastStart Taq DNA Polymerase, 50U FastStart Taq DNA Polymerase, 500U FastStart Taq DNA Polymerase, 5,000U (20 x 250U) GC RICH PCR 100U GC Rich PCR 100U High Fidelity PCR Master 1 Kit (200 rxns 50µl final vol.) Pwo DNA Polymerase 100U Pwo DNA Polymerase 500U Pwo Superyield DNA Polymerase 100U Pwo SuperYield DNA Polymerase 100U Pwo SuperYield DNA Polymerase 500U Taq DNA Polym. GMP Grade, (5 U/µL) 1,000U
5 Taq DNA Polym. GMP Grade, (5 U/µL) 1,000U Taq DNA Polymerase 2,500U Taq DNA Polymerase 5,000U (20x250 U) Taq DNA Polymerase (1u/µl) 1,000U (4 x 250U) Taq DNA Polymerase (1u/ul) 250 U 250U Taq DNA Polymerase 100U Taq DNA Polymerase 1,000U (4 x 250U) Taq DNA Polymerase 2,500U Taq DNA Polymerase 500U Taq DNA Polymerase 5,000U (20 x 250U) Taq DNA Polymerase, (1U/µl) 250U Taq DNA Polymerase (1U/µl) 1,000U (4x250U) Taq DNA Polymerase 100U Taq DNA Polymerase 500U Taq DNA Polymerase 1,000U (4 x 250U)
6 Restriction Enzymes and Buffers Description Buffers SuRE/Cut Buffer A 5 x 1ml SuRE/Cut Buffer B 5 x 1ml SuRE/Cut Buffer H 5 x 1ml SuRE/Cut Buffer L 5 x 1ml SuRE/Cut Buffer M 5 x 1ml SuRE/Cut Buffer Set for Restr. Enzymes 1 Set Restriction Enzymes Meganuclease I-Sce I 1,000U Aat II (1-5U/µl) 250U Acc I 500U Acy I (Aha II) 200U Afl III 100U Alu I (10U/µl) 2,000U Alu I (10U/µl) 500U Apa I (40U/µl) 20,000U Apa I (10U/µl) 5,000U Asp 700 (Xmn I) (10U/µl) 500U Asp 718 (10U/µl) 1,000U Asp 718 (10U/µl) 5,000U Asp 718, conc (40U/µl) 5,000U Asp EI (10U/µl) 200U Asp I (Tth 111 I) (10U/µl) 400U Ava I 1,000U Ava II 500U Avi II (10U/µl) 200U Bam HI (10U/µl) 10,000U Bam HI (10U/µl) 1,000U Bam HI (10U/µl) 2,500U Bam HI, conc. (40U/µl) 10,000U Bam HI,conc. (40U/µl) 50,000U Bbr PI (Pma CI) (10U/µl) 500U Bcl I, (10U/µl) 500U Bcl I, conc. (40U/µl) 2,500U Bfr I (Afl II) (10U/µl) 500U Bgl I, (10U/µl) 1,000U Bgl I, conc. (40U/µl) 5,000U Bgl II (10U/µl) 2,000U Bgl II (10U/µl) 500U Bgl II, conc. (40U/µl) 10,000U Bgl II, conc. (40U/µl) 2,000U Bln I (Avr II) (10U/µl) 200U Bln I (Avr II) (10U/µl) 1,000U
7 Bpu AI (10U/µl) 200U Bse AI (10U/µl) 200U Bsi WI (10U/µl) 300U Bsi YI (10U/µl) 200U Bsm I (10U/µl) 200U Bsp LU 11I (10U/µl) 200U Bss HII (10U/µl) 200U Bst 1107 I (10U/µl) 200U Bst EII (10U/µl) 500U Bst XI (10U/µl) 250U Bst XI (10U/µl) 1,250U Cell II (10U/µl) 200U Cfo I (Hha I) (10U/µl) 1,000U Cla I (10U/µl) 2,500U Cla I (10U/µl) 500U Cla I, conc. (40U/µl) 2,500U Dde I (10U/µl) 1,000U Dpn I (10U/µl) 1,000U Dpn I (10U/µl) 200U Dra I (10U/µl) 5,000U Dra II (1-3U/µl) 250U Dra III(1-5U/µl) 500U Ecl XI (Xma III) (10U/µl) 1,000U Eco 47III 100U Eco RI (10U/µl) 10,000U Eco RI (10U/µl) 5,000U Eco RI, conc. (40U/µl) 10,000U Eco RI, conc. (40U/µl) 50,000U Eco RII (Bst NI) (10U/µl) 200U Eco RV (10U/µl) 10,000U Eco RV (10U/µl) 2,000U Eco RV, conc. (40U/µl) 10,000U Fok I (1-5U/µl) 100U Hae III (10U/µl) 5,000U Hind II (3-10U/µl) 2,500U Hind III (10U/µl) 10,000U Hind III (10U/µl) 5,000U Hind III, conc. (40U/µl) 10,000U Hind III, conc. (40U/µl) 50,000U Hinf I (10U/µl) 1,000U Hinf I (10U/µl) 5,000U Hinf I, conc. (40U/µl) 20,000U Hpa I, (3-10U/µl) 100U Hpa I, (3-10U/µl) 500U Hpa II (10U/µl) 5,000U Hpa II, conc. (40U/µl) 5,000U Ita I (10U/µl) 200U
8 Kpn I (40U/µl) 10,000U Kpn I (10U/µl) 5,000U Ksp I (10U/µl) 1,000U Mae I (1-5U/µl) 250U Mae II (1-5U/µl) 50U Mae III (1-5U/µl) 250U Mae III (1-5U/µl) 50U Mlu I (10U/µl) 2,500U Mlu I (10U/µl) 500U Mlu I, conc. (40U/µl) 2,500U Mlu NI (10U/µl) 200U Mro I (Acc II) (>1-5U/µl) 100U Msp I (10U/µl) 5,000U Msp I, conc. (40U/µl) 5,000U Mun I (10U/µl) 200U Mva I (Bst NI) (10U/µl) 5,000U Mvn I (Fnu DII) (10U/µl) 200U Nae I (10U/µl) 1,000U Nar I (10U/µl) 1,000U Nco I (10U/µl) 1,000U Nco I (10U/µl) 200U Nco I, conc. (40U/µl) 1,000U Nde I (10U/µl) 1,000U Nde I (10U/µl) 200U Nde II (Mbo I) 1,000U Nhe I (10U/µl) 1,000U Nhe I (10U/µl) 200U Nhe I, conc. (40U/µl) 1,500U Not I (10U/µl) 1,000U Not I (10U/µl) 200U Not I, conc. (40U/µl) 1,000U Nru I (10U/µl) 1,000U Nsi I (10U/µl) 1,000U Nsi I (10U/µl) 200U Nsp I (10U/µl) 200U Pin AI, 1000 u Pin AI, 200 u Pst I (10U/µl) 10,000U Pst I (10U/µl) 3,000U Pst I, conc. (40U/µl) 10,000U Pvu I 100U Pvu I 500U Pvu II (10U/µl) 1,000U Pvu II (10U/µl) 5,000U Rca I (Bsp HI) 200U Rsa I (10U/µl) 1,000U Rsa I (10U/µl) 5,000U
9 Rsa I, conc. (40U/µl) 5,000U Rsr II (10U/µl) 200U Sac I (Sst I) (10U/µl) 1000U Sac I (Sst I) (10U/µl) 5,000U Sac I, conc. (40U/µl) 5,000U Sal I (10U/µl) 2,500U Sal I (10U/µl) 500U Sal I, conc. (40U/µl) 2,500U Sau3A I (1-5U/µl) 500U Sca I (10U/µl) 2,500U Sca I (10U/µl) 500U Sca I,conc. (40U/µl) 5,000U Sex AI (10U/µl) 200U Sfi I (10U/µl) 1,250U Sfi I, conc. (40U/µl) 5,000U Sfu I (Asu II) (10U/µl) 2,000U Sgr AI (10U/µl) 200U Sma I, (10U/µl) 1,000U Sma I, (10U/µl) 5,000U Sma I, conc. (40U/µl) 5,000U Sna BI (10U/µl) (10U/µl) Spe I (10U/µl) 200U Spe I (10U/µl) 1,000U Spe I, conc. (40U/µl) 1,000U Sph I (10U/µl) 200U Sph I (10U/µl) 2,500U Sph I (10U/µl) 500U Sph I, conc. (40U/µl) 2,500U Ssp I (10U/µl) 1,000U Ssp I, conc. (40U/µl) 1,000U Stu I (10U/µl) 2,500U Stu I (10U/µl) 500U Stu I, conc. (40U/µl) 2,500U Sty I (10U/µl) 2,000U Swa I (10U/µl) 200U Taq I (10U/µl) 10,000U Taq I (10U/µl) 2,500U Tru 9I (10U/µl) 1,000U Van 91 I (Pfl MI) 200U Xba I (10U/µl) 1,000U Xba I (10U/µl) 20,000U Xba I (10U/µl) 5,000U Xba I, conc. (40U/µl) 20,000U Xho I (10U/µl) 5,000U Xho I, conc. (40U/µl) 12,500U Xho I, conc. (40U/µl) 2,500U Xho II (1-5U/µl) 50U
10 Description Xma CI (10U/µl) 200U
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