Thermo Scientific Dharmacon sirna Libraries

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1 Thermo Scientific Dharmacon sirna Libraries Scientific innovation in sirna potency, specificity and delivery Superior sirna in comprehensive collections for reliable screens Market leading products for guaranteed, high-confidence results

2 Innovative products empower RNAi screening excellence Thermo Scientific sigenome, ON-TARGETplus, and Accell pre-designed sirna products are founded on our years of ground-breaking, published scientific discoveries that solve real-world problems in RNAi. sigenome : most trusted for superior silencing since 22. The most widely used, most cited sirna in the world; recently revised to reflect updated sequence database entries ON-TARGETplus : the standard for specificity. The only sirna with a unique, patented dual-strand modification pattern for unmatched specificity, featuring a seed region modification to minimize seed-related off target effects Accell : the breakthrough in delivery that changes the world of RNAi. Modified sirna for delivery into difficult-to-transfect cells without viral vectors, transfection reagents, or instrumentation Target mrna Levels (%) Target mrna Levels (%) 1 1 Screening with Thermo Scientific sigenome sirna HeLareagents provides effective target silencing >9% knockdown 8-89% knockdown 75-79% knockdown 7-74% knockdown <7% knockdown > 9% 8-89% 75-79% 7-74% < 7% Target Target Genes MCF1a HeLa MCF1a Effective silencing achieved for 44 kinase genes when screened with sirna reagents in two cell lines. Under screening conditions these overall results provide validation of sirna design and technology for effective target knockdown. HeLa and MCF1a cells were transfected with 5 nm of each sirna using Thermo Scientific DharmaFECT 1 transfection reagent. Target mrna levels were determined by branched DNA assay (Panomics Quantigene Reagent System). The pie chart represents the summary of silencing achieved for the entire data set (88 total data points). The bar graph is a representative sample of genes assayed. ON-TARGETplus reagents provide the most specific silencing available # Off-Target Genes Individual sirna ON-TARGETplus Individual sirna ON-TARGETplus ON-TARGETplus modifications reduce the overall number of off-targets, and pooling reduces them even further. Off-targets induced by the indicated sirna reagents targeting 2 human genes were quantified by gene expression microarray analysis (Agilent 22K Platform) and are represented in this boxplot (genes down-regulated by two-fold or more). Each box represents the middle 5% of the data set. Horizontal line in box: Median value of the data set. Vertical bars: Minimum and maximum data values. A B Accell sirna delivers silencing to difficult-to-transfect cells SH-SY5Y Accell GAPD SH-SY5Y Jurkat Jurkat Neuroblastoma cells (SH-SY5Y; panel A) and T-lymphocyte cells (Jurkat; panel B) were treated with 1 µm Accell Red Non-targeting sirna (Cat # D-196-1) and 1 µm Accell human GAPDH Control pool (Cat # D-193-1) in Accell delivery media (Cat# B-5) for 72 hours. Live cells were imaged by epifluorescence to demonstrate effective cellular uptake of the labeled control (Red; cytoplasm = Accell NTC Red and Blue; nucleus = Hoechst). GAPDH knockdown was assessed by ABgene Verso SYBR Green qrt-pcr Kit. The average expression levels of three biological replicates is shown. Beta-Actin was used as a housekeeping gene for normalization. Expression levels were further normalized to NTC. mrna Expression (%) mrna Expression (%) Accell GAPDH Accell GAPD Accell GAPDH

3 Novel design and pooling technologies advance sirna specificity Our sirna design algorithms incorporate strategies described by our product scientists in peerreviewed publications 1, 2. These groundbreaking methods enhance specificity of silencing by reducing off-target effects caused by the sirna seed region: Advanced design filters to exclude sirnas with conserved microrna seed-region motifs Preference for lower-frequency seed regions for minimization of off-target effects sirna designs with low-frequency seed regions ensure fewer off-targets Number of Off-Targets sirnas with low seed frequency have a significantly lower number of offtargets than sirnas with medium or high frequency seeds. Five sirnas with low, medium, or high frequency seed regions were transfected into HeLa cells and their associated off-target signatures assessed via global expression profiling (Agilent 22K platform). sirna sequences were constant in the duplex at positions corresponding to 1 and 8-19 in the antisense strand; only the seed regions (positions 2-7) were altered (see below). Low frequency seeds: <35 occurrences in the HeLa transcriptome. Medium frequency: occurrences. High frequency: >38 occurrences. Figure adapted from Anderson et.al. See published citation for full experimental details. 2 Low Medium High Seed Frequency Class 3 seed region of antisense strand (positions 2-7) 5' 3' Antisense Strand Sense Strand 3' 5' Birmingham, A., et al. 3 -UTR seed matches, but not overall identity, are associated with RNAi off -targets, Nature Methods. 3.3 (26): Anderson, et al.: Experimental validation of the importance of seed frequency to sirna specificity. RNA 14.5 (28). All Thermo Scientific sirna libraries are available as sirna reagents Pooling of sirnas better mimics the natural RNAi pathway and when combined with Thermo Scientific SMARTselection design, pooling provides advantages for both potency and specificity. Avoid unknown SNP regions and reduce false negatives by targeting four mrna regions at once The only method for reducing the effective concentration of a single sirna (a widely accepted strategy for reducing off-target effects) without compromising overall silencing efficiency sirna reagents improve specificity when retaining high potency Target mrna sirna 1 sirna 2 sirna 3 sirna 4 AAAA Use of a single, highly functional reagent will save screening time and money Target sirna 1 sirna 2 sirna 3 sirna 4 sirna Microarray analysis demonstrates potency and reduced off-targets. Off-target signatures and target silencing of sirna reagent and its four constituent sirnas, from total RNA of HeLa cells 24 hours posttransfection. Green indicates mrna reduced by 2-fold or more (Agilent 22K Platform).

4 The trusted partner of choice for more genome-wide screens Thermo Scientific Human and Mouse Druggable Genome and Whole Genome sirna libraries have been placed in leading research institutions worldwide. Landmark publications demonstrate the contribution of these important research tools to the basic understanding of disease states. 3,4 Druggable Genome includes highly relevant targets for superior screening results Kinases Ubiquitin conjugation (subsets 1, 2, 3) Proteases Drug Targets Enzymes Transcription Regulators GPCR Ion channels Phosphatases Autophagy Anti-apoptosis Cell Cycle Apoptosis Signaling Nuclear Receptors Senescence Translation Regulator DNA Repair Transporters Transmembrane Receptors GPCR Signaling The Human Druggable Genome sirna libraries consist of high-confidence gene targets based on recent publications that characterize potential druggable targets. The collection was assembled with use of innovative bioinfomatics tools to analyze the latest genetic and ontological information from multiple databases. 3 Brass, L. et al. Identification of host proteins required for HIV infection through a functional genomic screen Science (28): /science Tang, W. et al A genome-wide RNAi screen for Wnt/B-Catenin pathway components identifies unexpected roles for TCF transcription factors in cancer PNAS 15:28; (28): A broad selection to meet your RNAi screening needs Thermo Scientific sirna libraries are available as reagents or Set of Four individual sirnas in collections arranged by gene family and function. Please inquire if your desired library or product type is not indicated in the table below. HUMAN MOUSE sigenome ON-TARGETplus Accell Duet sigenome ON-TARGETplus Accell GPCR x Kinase x Ion Channels x Proteases x Phosphatases x Ubiquitin conjugation subset # x Ubiquitin conjugation subset # x Ubiquitin conjugation subset # x Nuclear receptors x 46 Deubiquitinating enzymes x Tyrosine kinases Cell cycle regulation Membrane trafficking Transcription factors 1,53 1,44 Drug Targets 4,795 4,786 4,799 x 7,1 Druggable Genome 7,59 7,567 7,587 x 1,6 6,757 Genome 1,582 1,537 x 19,61 Gene number may fluctuate as libraries undergo revision or when gene records are updated. Check for up-to-date product information and additions to our growing selection.

5 Rapid sirna screening - Simplified Patent-pending Thermo Scientific Reverse Transfection Format (RTF) sirna libraries are ideal for the lab that wants to screen with sirna, but doesn t have high-throughput capabilities. No sirna dispensing required; just resuspend the sirna and add your cells. sirna and controls provided in six single-use replicate plate sets - ready to use at 6.25 pmol per well Pre-defined Optimization Plates available to determine the best experimental conditions Available in white- or black-walled plates to support fluorescent or luminescent assays Duet sirna Libraries raise confidence by the power of 2 Thermo Scientific Duet libraries are paired human collections of sigenome and ON-TARGETplus sirna reagents, providing knockdown by the two most reliable sirna reagents available. Raise confidence in primary screen results with two sirna reagents per gene Move higher confidence hits through the screening pipeline faster Leverage ON-TARGETplus premium specificity in the primary screen to reduce false positives Primary Screen To identify potential gene targets Individual sirnas Duet sigenome & ON-TARGETplus Hit Stratification Phenotype confirmation with multiple reagents Individual sirnas Second Additional sirnas Target Validation Multiple assays Additional cell types Phenotype rescue Pathway mapping In silico analysis Duet sirna libraries support high-confidence screening workflows Offering more guaranteed sirna reagents than any other provider to enable the broadest range of sirna screening strategies. By leveraging the highefficiency silencing of market-leading Dharmacon sirna reagents, the execution of a Duet sirna screen with two unique sirna reagents (sigenome and ON-TARGETplus) increases overall primary hit quality and reduces follow-up experimental time and effort. Custom libraries for unique experimental requirements Don t see a gene collection of interest? Ready to follow-up on hits from a previous screen? Submit your gene list for custom library fulfillment in one of the following 96-well formats (2-well minimum order): One or more aliquots of.1-5 nmol sirna reagent per well Eight or more single-use RTF plates Submit an online quote request at For additional information or assistance in placing custom sirna library orders, contact Technical Support at techservice.genomics@thermofisher.com or your local sales representative.

6 Thermo Scientific Dharmacon sirna Libraries For further information visit: Technical Support: US: Europe: Ordering Information: North America 265 Crescent Dr., Suite 1 Lafayette, CO 826 Toll free: Tel: Fax: Belgium Tel: Fax: France Tel: Fax: Germany Tel: Fax: The Netherlands Tel: Fax: United Kingdom Tel: Fax: Switzerland Tel: Fax: Other Countries: Technical support: US Literature # 2-8-G-2-U Euro Literature # PB Thermo Fisher Scientific Inc. All rights reserved. QuantiGene is a trademark of Bayer. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.