Nathalie Le Roy. Doctor in Biology

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1 : Scientific Centre of Monaco 8, quai Antoine Ier Monaco : : nleroy@centrescientifique.mc Date of birth: 04/04/1983 Nathalie Le Roy Doctor in Biology Molecular biology, Biochemistry, Evolution, Biomineralization CURRENT POSITION PhD/Postdoc in marine biology Scientific Centre of Monaco SKILLS BIOCHEMISTRY MOLECULAR BIOLOGY Extraction of proteins, Electrophoresis, coloration of gels, Immuno labelling (Western blot & immunogold), Tests of enzymatic activity Primer design, Extraction of RNA & DNA RT PCR, 5 & 3 RACE, qpcr, Ligation, construction & screening of cdna libraries, WMISH (whole mount in situ hybridization) MICROSCOPY BIOINFORMATIC Scanning Electronic Microscopy Natural & fluorescent lights microscopy Confocal Laser Scanning Microscopy (initiation) BLAST in databases, in silico analyses, Sequence alignments (Se Al & ClustalW), Phylogenetic analyses (PhyML & MrBayes), ProTest & GeneDoc FIELD/DISSECTION INFORMATIC Fishing, Vertebrates & invertebrates dissection Photoshop, Illustrator, Word, Excel, Powerpoint CELL CULTURE/MICROBIOLOGY LANGUAGES Culture of mouse C2C12 (initiation) & transformation/culture of E. coli English (reading, writing and speaking) Spanish (college level) 2

2 PhD Thesis PhD thesis: Carbonic anhydrase in calcium carbonate biomineralizations: activity, expression and evolution. Supervisor: Dr. Frédéric Marin Laboratory: Biogeosciences, University of Burgundy (UB), Dijon, France Mention: with high honours EDUCATION Master degree in Systematic, Evolution and Palaeontology University of Pierre et Marie Curie (UPMC, Paris, France) BsC in Life Sciences, Ecology and Evolution UPMC (Paris, France) MASTER INTERNSHIPS 2007 (6 month) M2 Master internship: Bonegrowthratepredictioninextinctamniotesusing a paleobiological model. Supervisor: Pr. Jorge Cubo / UPMC (Paris, France) 2006 (3 month) M1 Master internship: Relationship between the metabolic rate and the bone growth rate in extant amniotes. Supervisors: Pr. J. Cubo & Dr. L. Montes / UPMC (Paris, France) SCIENTIFIC COLLABORATIONS 2012 (2 weeks) Internship for in situ in toto hybridization initiation with Dr. Daniel Jackson / University of Gottingen (Germany) 2010 (10 days) Mission in Cuba for carbonate environments studies with Pr. Emmanuelle Vennin & Dr. Christophe Durlet / UB (Dijon, France) 2009 (3 months) Internship in molecular biology with Dr. Jean Yves Sire / UPMC (Paris, France) 2008 (1 month) Internship in molecular biology with Dr. Sergio Buccary / University of Chile (Santiago, Chile) TEACHING & VULGARIZATION Sept to Jan February to April 2011 Junior lecturer in biology / University of Limoges (France) Temporary teacher in palaeontology / UB (Dijon, France) 2009 (4 month) Formation assistant of a PhD student (Paula Silva) / UB (Dijon, France) August 2008 Supervisor of a BsC student (Cédric Tallobre) / UB (Dijon, France) Expérimentarium Scientific vulgarization / UB (Dijon, France) 3

3 LIST OF PUBLICATIONS A) Publications with peer review process (1) Luquet, G., Fernández, M.S., Badou, A., Guichard, N., Le Roy, N., Corneillat,M.,Alcaraz,G.and Arias, J.L. (2013). Comparative ultrastructure and carbohydrate composition of gastroliths from astacidae, cambaridae and parastacidae freshwater crayfish (Crustacea, Decapoda). Biomolecules, 3: (2) Legendre, L., Le Roy, N., Martinez Maza, C., Montes, L., Laurin, M.and Cubo, J. (2013). Phylogenetic signal in bone histology of amniotes revisited. Zoologica Scripta, 42: (3) Le Roy, N., Marie, B., Gaume, B., Guichard, N., Delgado, S., Zanella Cléon, I., Becchi, M., Auzoux Bordenave, S., Sire, J. Y. and Marin, F. (2012). Identification of two carbonic anhydrases in the shellforming mantle of the European abalone Haliotis tuberculata (Gastropoda, Haliotidae): phylogenetic implications. J Exp Zool B., 318: (4) Ramos Silva, P., Ben Hamada, S., Le Roy, N., Marie, B., Guichard, N., Zanella Cléon, I., Plasseraud, L., Corneillat, M., Alcaraz, G., Kaandorp, J. and Marin, F. (2012). Novel molluskan biomineralization proteins retrieved from proteomics: a case study with Upsalin. ChemBioChem, 13(7): (5) Cubo, J., Le Roy, N., Martinez Maza, C. and Montes, L. (2012). Evolution of bone growth rate in Archosaurs. Paleobiology 38: (6) Marin, F., Le Roy, N. and Marie, B. (2012). The formation and mineralization of mollusk shell. Frontiers in Biosciences S4: (7) Marie, B., Le Roy, N., Zanella Cléon, I., Becchi, M. and Marin, F. (2011). Molecular evolution of mollusc shell proteins: Insights from proteomic analysis of the edible mussel Mytilus.JMolEvol,72: (8) Marie, B., Zanella Cléon, I., Le Roy, N., Becchi, M., Luquet, G. and Marin, F. (2010). Proteomic analysis of the acid soluble nacre matrix of the bivalve Unio pictorum: detection of novel carbonic anhydrase and putative protease inhibitor proteins. ChemBioChem, 11: (9) Montes, L., Le Roy, N., Perret, M., Buffrenil, V. de, Castanet, J. and Cubo, J. (2007). Relationships between bone growth rate, body mass and resting metabolic rate in growing amniotes: a phylogenetic approach. Biol J Linn Soc, 92(1): B) Chapter of book Marin, F., Marie, B., Ben Hamada, S., Silva, P., Le Roy, N., Guichard, N., Wolf, S., Montagnani, C., Joubert, C., Piquemal, D., Saulnier, D., Gueguen, Y. (2013). Shellome : proteins involved in mollusc shell biomineralization diversity, functions. In Recent Advances in Pearl Research. Edited by Watabe S, Maeyama K, Nagasawa H. p

4 C) Publications in proceedings with peer review process (1) Marin, F., Marie, B., Ben Hamada, S., Silva, P., Le Roy, N., Guichard, N., Wolf, S., Montagnani, C., Joubert, C., Piquemal, D., Saulnier, D., Gueguen, Y. (2011). Shellome : proteins involved in mollusc shell biomineralization diversity, functions. In Recent advances in pearl research Proceedings of the International Symposium on Pearl Research, The University of Tokyo, Tokyo, Japan, October 4 6, P (2) Luquet, G., Le Roy, N., Zanella Cléon,I.,Becchi,M.,Bucarey,S.,Fernandez,M.S.,Arias,J.L.,Guichard, N., Marie, B., Marin, F. (2009). Characterization of crustacyanin A2 subunit as a component of the organic matrix of gastroliths from the crayfish Cherax quadricarinatus. In: Structure property relationships in biomineralized and biomimetic composites (eds Kisailus D, Estroff L, Gupta H, Landis W, Zavattieri P), MRS symposium Proceedings, San Fransisco, USA, April 13 17, 1187: 3 8. (3) Marie, B., Le Roy, N., Marie, A., Dubost, L., Milet, C., Bédouet, L., Becchi, M., Zanella Cléon, I., Jackson, D., Degnan, B., Luquet, G., Marin, F. (2009). Nacre evolution: A proteomic approach. In: Structureproperty relationships in biomineralized and biomimetic composites (eds Kisailus D, Estroff L, Gupta H, Landis W, Zavattieri P), MRS symposium Proceedings, San Fransisco, USA, April 13 17, 1187: NATIONAL & INTERNATIONAL CONGRESS (1) Le Roy N, Jackson D, Ramos Silva P, Marie B, Marin F (2013). Evolution of metazoan carbonic anhydrases and their recruitment to roles in CaCO 3 biomineralization. 12 th International Symposium on Biomineralization, Freiberg, Allemagne, p23. (oral communication) (2) Le Roy N, Jackson D, Ramos Silva P, Marie B, Marin F (2013). Evolution of metazoan carbonic anhydrases and their recruitment to roles in CaCO 3 biomineralization. 2 nd workshop of the COST Action TD0903, Bologne, Italie. (oral communication) (3) Le Roy N, Gaume B, Guichard N, Auzoux Bordenave S, Sire J Y, Marin F (2012). Carbonic anhydrase, CaCO 3 biomineralization, and metazoan evolution. 22 nd Goldschmidt Geochemistry Conference, Montreal, Canada, p346. (4) Le Roy N, Silva P, Marie B, Marin F (2011). The evolution of the α carbonic anhydrase family: the particular case of calcium carbonate biomineralizations in non vertebrate metazoans. 13 th Congrès Français de Sédimentologie, Dijon, France, p342. (oral communication) (5) Le Roy N, Gaume B,Marie B,Guichard N,Delgado S,Zanella Cléon I, Becchi M, Auzoux Bordenave S, Sire J Y, Marin F (2011). Identification de deux anhydrases carboniques dans le manteau de l ormeau européen Haliotis tuberculata (Gastropoda, Haliotidae) : implications phylogénétiques. 13 th Journée Française de Biologie des Tissus Minéralisés, Paris, France, p17. (oral communication) (6) Le Roy N, Marie B, Gaume B, Guichard N, Auzoux Bordenave S, Delgado S, Sire J Y, Marin F (2010). Carbonic anhydrase in the biomineralization of the shell of the gastropod Haliotis tuberculata (European ormer). Gordon Research Conference on Biomineralization, Colby Sawyer College, New London, NH, USA. (poster) 5

5 (7) Le Roy N, GaumeB,GuichardN,Auzoux Bordenave S, Marie B, Luquet G, Marin F (2010). L anhydrase carbonique dans la biominéralisation de la coquille du gastéropode Haliotis tuberculata. 12 th Journée Française de Biologie des Tissus Minéralisés, Saint Etienne. Abstract book, p63. (poster award) (8) Le Roy N, GaumeB,GuichardN,Auzoux Bordenave S, Marie B, Luquet G, Marin F (2010). Carbonic anhydrase (CA) in the shell biomineralization process of the gastropod Haliotis tuberculata.1 st workshop of the COST Action TD0903, Pula, Croatia. (oral communication) (9) Le Roy N, Guichard N, Marie B, Marin F, Luquet G (2009). Anhydrase carbonique et biominéralisation carbonatée chez les crustacées et les bivalves. 15 th Forum des Jeunes Chercheurs, University of Burgundy, France. (poster award) (10) Le Roy N, Bucarey S, Zanella Cléon I, Becchi M, Guichard N, Fernandez MS, Arias JL, Marie B, Marin F, Luquet G (2009). Analyse structurale et moléculaire des gastrolithes, structures de stockage de calcium, chez l écrevisse Cherax quadricarinatus.11 th Journée Française de Biologie des Tissus Minéralisés, Nice, France, p27. (poster award) (11) Le Roy N & Cubo J (2007). A predictive model of paleobiological estimation of bone growth rate from bone tissu types in extinct amniotes. 8 th International Congress of Vertebrate Morphology, Paris, France, p1098. (poster) INTERESTS Sports: Swimming; Art: Drawing, painting; Literature and Cinema: Science fiction Music: Electric and Acoustic Guitar Snorkelling: coral barrier reef in Cuba (2010); Great Barrier Reef in Australia (2012; Heron island and Againcourt reef) Association:MemberofShark Angels France (protection of sharks) 6