1 American Journal of Botany 89(11): OCCURRENCE AND PHYLOGENETIC SIGNIFICANCE OF LATEX IN THE MALPIGHIACEAE 1 ANDREA S. VEGA, 2,5 MARIA A. CASTRO, 3,5 AND WILLIAM R. ANDERSON 4,5 2 Instituto de Botánica Darwinion, Labardén 200, C. C. 22, B1642HYD Buenos Aires, Argentina; 3 Laboratorio de Anatomía Vegetal, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA Buenos Aires, Argentina; and 4 University of Michigan Herbarium, Suite 112, 3600 Varsity Drive, Ann Arbor, Michigan USA Latex and laticifers are reported for the first time in the genera Galphimia and Verrucularia (Malpighiaceae), with description and illustration of the leaf and stem anatomy of both genera. Those genera and the other two in which latex is known (Lophanthera and Spachea) constitute a single tribe, Galphimieae, that is at or near the base of the family s phylogeny, which suggests that latex in the Malpighiaceae may indicate an ancestor shared with the Euphorbiaceae. Key words: Galphimia brasiliensis; Galphimieae; latex; laticifers; Malpighiaceae; Verrucularia glaucophylla. The angiosperm family Malpighiaceae includes approximately 65 genera and 1250 species in the world and is distributed in tropical and subtropical regions of both hemispheres (W. R. Anderson, unpublished data). Nearly 950 species are endemic to the New World, northern South America being the major center of diversity (Anderson, 1979). The monophyly of the Malpighiaceae is supported by morphological characters (Anderson, 1979, 1990) and has recently been confirmed with molecular data (Chase et al., 1993; Cameron, Chase, and Anderson, 1995; Wurdack and Chase, 1996). Recent molecular studies of the Malpighiaceae did not provide convincing bases for dividing it into a small number of monophyletic subfamilies or tribes that reflect obvious morphological synapomorphies (Cameron et al., 2001; Davis, Anderson, and Donoghue, 2001), but they did confirm the monophyly of the tribe Galphimieae Nied. This tribe, as circumscribed by Anderson (1978), comprises four genera: Galphimia Cav., Lophanthera A. Juss., Spachea A. Juss., and Verrucularia A. Juss. Anderson (1981, 2001) mentioned the presence of milky latex in the stems of Lophanthera and Spachea, and the present paper documents the occurrence of latex in the other two genera, Galphimia and Verrucularia. Galphimia includes approximately ten species of tropical and subtropical America, with a disjunct distribution (Niedenzu, 1928). Most of the species grow in Mexico and Central America and only one, Galphimia brasiliensis (L.) A. Juss., is found in South America, in northeastern Argentina, southern Brazil, Bolivia, Paraguay, and Uruguay. This species has leaf and calyx glands, and the structural and ultrastructural anatomy of these glands was studied by Castro, Vega, and Múlgura (2001). The genus Verrucularia A. Juss. includes two species, V. glaucophylla A. Juss. and V. piresii W. R. Anderson. Verrucularia glaucophylla has only calyx glands and is endemic to Bahia, Brazil (Niedenzu, 1928). 1 Manuscript received 22 January 2002; revision accepted 21 May The authors thank André M. Amorim for his valuable help in collecting materials of Verrucularia glaucophylla and graphic designer Tomás E. Aversa for his technical assistance. 5 Authors for reprint requests ( fcen.uba.ar; MATERIALS AND METHODS Plant materials Fresh and fixed materials of Galphimia brasiliensis (L.) A. Juss. and Verrucularia glaucophylla A. Juss. were studied. Herbarium specimens were deposited in the herbarium of the Instituto de Botánica Darwinion (SI). Studied materials: Galphimia brasiliensis: ARGENTINA. Prov. Entre Ríos. Dpto. Colón: Parque Nacional El Palmar, Los Loros, 14/III/1998, Múlgura & Vega 1991; 4/XII/1998, Múlgura & Vega Dpto. Concordia: Parque Rivadavia, near José Hernández monument, 15/III/1998, Múlgura & Vega Verrucularia glaucophylla: BRAZIL. Bahia. Municipio de Morro do Chapéu, Cachoeira do Ferro Doido, ca m, 4/V/2001, Amorim et al (CEPEC, SI). Anatomical studies Leaves and stems of G. brasiliensis and V. glaucophylla were fixed in FAA (formalin : ethanol : acetic acid) and then stored in 70% ethanol. Part of each sample had been previously treated with sodium hypochlorite to eliminate contents. All materials were dehydrated in an ethanol series and embedded in paraffin after infiltration in a vacuum oven. Transverse and longitudinal sections 10 m thick were cut on a rotary microtome. Sections were double stained with safranin-fast green (D Ambrogio de Argüeso, 1986). Light microscope studies were made using a ZEISS Phomi III microscope (Zeiss, Oberkochen, Germany) and black and white Kodak Tmax 25 ASA film. RESULTS Field work During field work in Entre Ríos province (Argentina), the presence of latex in G. brasiliensis was observed. The latex is milky and was detected in stems and blades (Figs. 1 2). During the preparation of the present paper, Ch. Anderson (personal communication) told us about one collection of G. brasiliensis from Bolivia (Ortíz S. 7, MICH) whose label reported it to produce latex. Collectors of herbarium specimens of V. glaucophylla have never reported observing latex in the field, but laticifers were detected by anatomical techniques in stems and blades of that species. Anatomical studies Galphimia brasiliensis The blade in transverse and longitudinal sections is shown in Figs The adaxial epidermis is one-layered, the cells quadrangular to rectangular, with a thick outer tangential cell wall and cuticle. The papillate abaxial epidermis has a thin outer tangential cell wall. Trichomes are absent. Stomata are present in both surfaces and are more abundant in the abaxial epidermis. The 1725
2 1726 AMERICAN JOURNAL OF BOTANY [Vol. 89 Figs Galphimia brasiliensis (L.) A. Juss Field photographs of latex in petiole and blade, respectively. Figs Blade in transverse section, with laticifers immersed in spongy parenchyma. 3. Cleared section. Bar 50 m. 4. Uncleared section. Bar 50 m. 5. Blade in longitudinal section showing an articulated and unbranched laticifer. Bar 50 m. Figs Stem. 6. Transverse section. Bar 50 m. 7. Longitudinal section. Bar 50 m. ABE, abaxial epidermis; ADE, adaxial epidermis; B, blade; C, cuticle; CH, chlorenchyma; CO, cortex; L, laticifer; LX, latex; P, petiole; PH, phloem; PI, pith; PP, palisade parenchyma; S, stomata; SP, spongy parenchyma; VB, vascular bundles; X, xylem. dorsiventral mesophyll includes one layer of palisade parenchyma, composed of axially elongated cells slightly attached to one another and representing one-third of the total thickness. The spongy parenchyma has conspicuous intercellular spaces in which are immersed abundant laticifers distributed in the proximity of collateral vascular bundles. The laticifers were easily detected in transverse and longitudinal sections of the blades (Figs. 3 5) and stained intensively in uncleared sections (Fig. 4). In transverse section, laticifers are circular, ( 35.1) m in diameter, numerous, with a parallel and regular pattern in relation to the axis of the leaf and (51.3 ) m apart (Fig. 3 4). In longitudinal section, laticifers are tubular, articulated, not ramified, and not anastomosing (Fig. 5). The cells of the laticifers are m long, elongated, with dilated extremes like a trumpet and semi-thick terminal walls. The stem in transverse and longitudinal sections is illustrated in Figs The stem is circular in shape and is bounded by a one-layered epidermis with stomata. The cortex consists of subepidermal spongy chlorenchyma and parenchyma tissue, then secondary phloem and xylem and a conspicuous pith of parenchyma cells. Laticifers are distributed in the cortex and are scarcely recognizable due to their similarity to parenchyma cells (Fig. 7). Verrucularia glaucophylla The blade in transverse and longitudinal sections is shown in Figs The adaxial epidermis is one-layered, the cells quadrangular, with a thick outer tangential cell wall and cuticle. The conspicuously papillate abaxial epidermis exhibits one simple or occasionally bifurcate papilla on each cell, and a thick outer tangential cell wall. Trichomes are absent. Stomata are present in the abaxial epidermis overarched by adjacent papillate epidermal cells. The mesophyll is dorsiventral, including one-layered palisade parenchyma, which represents about one-half of the total thickness. The cells of typical palisade parenchyma are axially elongated and attached to one another. The spongy parenchyma has conspicuous intercellular spaces. Abundant laticifers are immersed between the palisade and spongy parenchyma, in the prox-
3 November 2002] VEGA ET AL. LATEX IN MALPIGHIACEAE 1727 Figs Verrucularia glaucophylla A. Juss. 8. Blade in transverse section showing laticifers immersed in the mesophyll. Bar 50 m. Figs Blade in paradermal section. 9. Bar 50 m. 10. Bar 100 m. Figs Stem in transverse section. 11. Bar 100 m. 12. Cuticle, epidermis, incipient periderm, and cortical parenchyma in detail. Bar 20 m. 13. Secondary xylem and central pith of parenchyma showing a peripheral starchy cell zone in detail. Bar 20 m. 14. Stem in longitudinal section showing cortical laticifers (arrow). Bar 25 m. ABE, abaxial epidermis; ADE, adaxial epidermis; C, cuticle; CO, cortex; F, phloem fibers; L, laticifers; PH, phloem; PE, periderm; PP, palisade parenchyma; S, stomata; SE, stem epidermis; SP, spongy parenchyma; ST, starchy parenchyma cells; SX, secondary xylem; T, trichomes.
4 1728 AMERICAN JOURNAL OF BOTANY [Vol. 89 imity of collateral vascular bundles. The laticifers were easily detected in both transverse and longitudinal sections of the blades (Figs. 8 10). In transverse section, the laticifers are circular, (31.2 ) ( 62.4) m in diameter, numerous, and (52 ) ( 176.8) m apart, with a parallel and irregular pattern in relation to the axis of the leaf (Fig. 8). In longitudinal section, the laticifers are tubular, articulated, not ramified, and not anastomosing. The cells of the laticifers are (369.2 ) ( 728) m long, uniform in diameter throughout their length (Fig. 9). In paradermal section the laticifers show a complex sinuous pattern (Fig. 10). The stem in transverse and longitudinal sections is illustrated in Figs The stem in early stages of secondary growth is circular in shape and surrounded by a one-layered epidermis with stomata (Fig. 11). The epidermal cells are radially elongated, with a thick outer tangential wall and cuticle. There is an incipient subepidermal periderm composed of 3 4 layers of cells (Fig. 12). The cortex contains 5 8 layers of parenchyma cells with laticifers and scarce starch and calcium oxalate druses. The vascular cylinder is composed of partially lignified phloem fibers, secondary phloem, and xylem. A central pith of parenchyma shows a peripheral zone of starchy cells (Fig. 13). Laticifers, present in the cortex, are easily recognized in longitudinal section (Fig. 14) but scarcely recognizable in transverse section because of their similarity to the parenchyma cells in which they are immersed. DISCUSSION Anderson (1978) restricted the tribe Galphimieae Nied. to four genera (Galphimia, Lophanthera, Spachea, and Verrucularia) on the basis of morphological characters. Cameron et al. (2001), using molecular data from the same four genera, found 100% bootstrap support for the tribe Galphimieae as restricted by Anderson, and Davis, Anderson, and Donoghue (2001) reported similar support, although their study lacked material of Verrucularia. Latex has previously been reported from only two genera of the Malpighiaceae, Lophanthera and Spachea (Anderson, 1981, 2001). The present study constitutes the first report of latex in Galphimia and Verrucularia and the first description to be published of the laticifers in this family. The fact that laticifers and latex are present in all four genera of the tribe Galphimieae provides a synapomorphy giving additional support to the monophyly of the tribe. There is an alternate, more interesting way to interpret the presence of latex in the tribe Galphimieae. The family Malpighiaceae was included in a large clade recently named Malpighiales (APG, 1998), with Violaceae, Passifloraceae, Linaceae, Clusiaceae, Peridiscaceae, and Euphorbiaceae, among others (Chase et al., 1993; Savolainen et al., 2000; Soltis et al., 2000). Anderson (1990) suggested that Lophanthera may be basal in the family, and the trees published recently (Cameron et al., 2001; Davis, Anderson, and Donoghue, 2001) place the tribe Galphimieae at or near the base of the family s phylogeny. It is therefore tempting to speculate that the presence of latex in this one tribe of Malpighiaceae constitutes a character linking the family to the Euphorbiaceae. In that interpretation, latex in the basal genera of the Malpighiaceae may be a symplesiomorphy inherited from a common ancestor shared with the Euphorbiaceae sensu stricto, i.e., the subfamilies Euphorbioideae and Crotonoideae of Webster (1975). Those are the groups of Euphorbiaceae that have laticifers, and they also share with the Malpighiaceae the character of having only one ovule in each locule of the ovary. The Euphorbioideae further resemble the Malpighiaceae in having simple leaves and simple hairs, their biglandular bracts recall the gland-bearing leaves and sepals of the Malpighiaceae, and their pollen is tricolporate, as in the basal clades of Malpighiaceae (Webster, 1975; Davis, Anderson, and Donoghue, 2001). The laticifers in the Euphorbioideae are non-articulated, whereas in the Crotonoideae, which in most characters are less like the Malpighiaceae, the laticifers are articulated like those reported here for the Galphimieae. Rudall (1987) argued that articulated and non-articulated laticifers probably evolved from a common ancestor in the Euphorbiaceae, and the evidence from the Malpighiaceae would seem to support the presence of articulated laticifers in the common ancestor of Euphorbiaceae sensu stricto and Malpighiaceae. Because the resolution of the phylogeny at the base of the Malpighiaceae is still rather labile, it would be best to regard the above phylogenetic speculations as preliminary, but they do point the way to future studies. It would be worthwhile to seek laticifers in the genera identified in the recent molecular studies (Cameron et al., 2001; Davis, Anderson, and Donoghue, 2001) as most closely related to the Galphimieae: Acmanthera Griseb., Blepharandra Griseb., Byrsonima L. C. Rich. ex Kunth, Coleostachys A. Juss., Diacidia Griseb., and Pterandra A. Juss. The fact that latex is unknown in those genera does not mean they do not have laticifers; latex had not been reported from Verrucularia, either, and we discovered its laticifers only because one of us (W. R. Anderson) predicted their existence on the basis of that genus s position in the tribe Galphimieae. If laticifers are basal in the Malpighiaceae, a symplesiomorphy shared with an outgroup like Euphorbiaceae, one would predict that laticifers, even if non-functional, may have been retained in some of the other genera near the base of the family s phylogeny. LITERATURE CITED ANDERSON, W. R [ 1977 ]. Byrsonimoideae, a new subfamily of the Malpighiaceae. Leandra 7: ANDERSON, W. R Floral conservatism in neotropical Malpighiaceae. Biotropica 11: ANDERSON, W. R Malpighiaceae. In The botany of the Guayana Highland: Part XI. Memoirs of the New York Botanical Garden 32: ANDERSON, W. R The origin of the Malpighiaceae the evidence from morphology. Memoirs of the New York Botanical Garden 64: ANDERSON, W. R Malpighiaceae. In P. E. Berry, K. Yatskievych, and B. K. Holst [eds.], Flora of the Venezuelan Guayana, vol. 6, Missouri Botanical Garden Press, St. Louis, Missouri, USA. APG [ANGIOSPERM PHYLOGENY GROUP] An ordinal classification for the families of flowering plants. Annals of the Missouri Botanical Garden 85: CAMERON, K. M., M. W. CHASE, AND W. R. ANDERSON Comparison of molecular trees from rbcl and matk analyses of Malpighiaceae. American Journal of Botany 82(supplement): 117 (Abstract). CAMERON, K. M., M. W. CHASE, W.R.ANDERSON, AND H. G. HILLS Molecular systematics of Malpighiaceae: evidence from plastid rbcl and matk sequences. American Journal of Botany 88: CASTRO, M. A., A. S. VEGA, AND M. E. MÚLGURA Structure and ultrastructure of leaf and calyx glands in Galphimia brasiliensis (Malpighiaceae). American Journal of Botany 88: CHASE, M. W., ET AL Phylogenetics of seed plants: an analysis of nucleotide sequences from the plastid gene rbcl. Annals of the Missouri Botanical Garden 80: D AMBROGIO DE ARGÜESO, A Manual de Técnicas en Histología Vegetal. Hemisferio Sur S.A., Buenos Aires, Argentina.
5 November 2002] VEGA ET AL. LATEX IN MALPIGHIACEAE 1729 DAVIS, C. C., W. R. ANDERSON, AND M. J. DONOGHUE Phylogeny of Malpighiaceae: evidence from chloroplast ndhf and trnl-f nucleotide sequences. American Journal of Botany 88: NIEDENZU, F Malpighiaceae. In A. Engler [ed.], Das Pflanzenreich IV, 141. W. Engelmann, Leipzig, Germany. RUDALL, P. J Laticifers in Euphorbiaceae a conspectus. Botanical Journal of the Linnean Society 94: SAVOLAINEN, V., M. W. CHASE, S.B.HOOT, C.M.MORTON, D.E.SOLTIS, C. BAYER, M. F. FAY, A. Y. DE BRUIJN, S. SULLIVAN, AND Y. L. QIU Phylogenetics of flowering plants based upon a combined analysis of plastid atpb and rbcl gene sequences. Systematic Biology 49: SOLTIS, D.E.,ET AL Angiosperm phylogeny inferred from 18S rdna, rbcl, and atpb sequences. Botanical Journal of the Linnean Society 133: WEBSTER, G. L Conspectus of a new classification of the Euphorbiaceae. Taxon 24: WURDACK, K. J., AND M. W. CHASE Molecular systematics of Euphorbiaceae sensu lato using rbcl sequence data. American Journal of Botany 83(supplement): 203 (Abstract).
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