KI8 >e jijwhj#?j ¹ Wcfb_\o edbo m^w j oek mwd j$

Transcription

1 H[lebkj_ed_p[ b_\[ KI8 >e jijwhj#?j ¹ Wcfb_\o edbo m^w j oek mwd j$ Affymetrix is proud to offer premium USB HotStart-IT qpcr reagents. And for a limited time, get a special discount of 50% off select products. Increase the sensitivity, consistency, and specificity of your real-time PCR and get the same exceptional service you expect from USB. Je ] [ j +& e\\ or to learn more about USB HotStart-IT qpcr reagents, call or visit In Europe: +49(0) or visit Refer to promo code 29H-1300 to take advantage of this offer. BioSupplyNet.com is your one-stop directory source for life science laboratory supplies and services. Concise and user-friendly, BioSupplyNet.com provides direct access to over 6500 companies and 20,000 products. FEATURES: Download FREE protocols from Cold Spring Harbor Protocols Search for the latest kits and catalogs See the latest products and special promotions Sign up for a free monthly newsletter detailing new protocols and products Visit our updated career center

2 H[lebkj_ed_p[ b_\[ KI8 >e jijwhj#?j ¹ Wcfb_\o edbo m^w j oek mwd j$ Affymetrix is proud to offer premium USB HotStart-IT qpcr reagents. And for a limited time, get a special discount of 50% off select products. Increase the sensitivity, consistency, and specificity of your real-time PCR and get the same exceptional service you expect from USB. Je ] [ j +& e\\ or to learn more about USB HotStart-IT qpcr reagents, call or visit In Europe: +49(0) or visit Refer to promo code 29H-1300 to take advantage of this offer. BioSupplyNet.com is your one-stop directory source for life science laboratory supplies and services. Concise and user-friendly, BioSupplyNet.com provides direct access to over 6500 companies and 20,000 products. FEATURES: Download FREE protocols from Cold Spring Harbor Protocols Search for the latest kits and catalogs See the latest products and special promotions Sign up for a free monthly newsletter detailing new protocols and products Visit our updated career center

3 2009 Research Antibody Catalog The Power to Question FR O M SAN TA CR UZ B I OT ECH N O LO GY, I N C. Genes & Development I N T R O D U CI N G T H E N E W Leading Worldwide Source for: monoclonal and polyclonal antibodies sirna and shrna (plasmid and lentivirus) products gene specific transfected cell lysates phospho-specific antibodies secondary antibodies and support products Volume 23 No. 7 April 1, 2009 A JOURNAL OF CELLULAR AND MOLECULAR BIOLOGY Linking autophagy and cellular senescence Website updated daily with new antibodies, sirna/ shrna products, transfected lysates, support data and product citations. 23(7): G & ed e v enl o p mee n t s Headquarters TOLL FREE: TEL: FAX: customercare@scbt.com European Support TOLL FREE: FAX: europe@scbt.com JAPAN TOLL FREE: (010) S. KOREA TOLL FREE: N. CHINA TOLL FREE: Santa Cruz Biotechnology, Inc. and the Santa Cruz Biotechnology, Inc. logo are registered trademarks of Santa Cruz Biotechnology, Inc. PRESS Cold Spring Harbor Laboratory Press G&D 200 S. CHINA TOLL FREE: April 1, 2009 Asian Support Also in this issue: p53 regulation by microrna-125b Targeting Rho1 during cytokinesis 9 PHONE:

4 Exiqon microrna services give you access to our proven mircury LNA array technology for reliable, robust results from every sample with as little as 200ng total RNA. You also get the broadest coverage of micrornas, including 435 human micrornas available nowhere else. For validation, our PCR Services will reliably quantitate any microrna, even AT-rich sequences and 3 -end variants. Hire the microrna experts and get scientific partnership that will exceed your expectations from consultation and analysis to final report. Hire Exiqon. Learn more Visit mircury LNA microrna Array mir-7b mir-7b mir-15b mir-15b mir-21 mir-21 mir-23b mir-23b mir-192 mir-192 mir-203 mir-203 mir-204 mir Two-way clustering of mircury LNA microrna Array and Real-time PCR data generate very similar heat maps. This is illustrated by the fact that the nine cell lines are clustered in the same way NOTICE TO PURCHASER: LIMITED LICENSE. Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. SYBR Green is a trademark of Invitrogen So why risk your samples with some big name assay when you can engage Exiqon for expert microrna services? mircury LNA microrna PCR System Cell line A Cell line B Cell line C Cell line D Cell line E Cell line F Cell line G Cell line H Cell line I Nobody knows micrornas better than Exiqon. And no microrna profiling or PCR system is as rigorous or as feature-rich as a system based on Exiqon s exclusive Locked Nucleic Acid (LNA ) technology. Reliable and robust publication-ready data Cell line A Cell line B Cell line C Cell line D Cell line E Cell line F Cell line G Cell line H Cell line I microrna discovery experts that will exceed even your rigorous expectations

5 The online source of trusted techniques in molecular and cell biology Contains cutting-edge and classic protocols presented step-by-step with cautions and troubleshooting Frequently updated and annotated Interactive, customizable, and fully searchable Subject Coverage Antibodies Bioinformatics/Genomics Cell Biology Chromatography Computational Biology DNA Delivery/Gene Transfer Electrophoresis Emerging Model Organisms Genetics High-Throughput Analysis Imaging/Microscopy Immunology Laboratory Organisms Molecular Biology Neuroscience Newly Added Protocols Plant Biology Polymerase Chain Reaction (PCR) Proteins and Proteomics RNA Interference (RNAi)/siRNA Stem Cells Transgenic Technology C old Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques. For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology. Now that access has moved online. Visit Cold Spring Harbor Protocols today and discover a rich, interactive source of new and classic research techniques. The site is fully searchable, with many tools that can be customized by users, including topic-based alerting and personal folders. Through a web-based editorial process, users also have the opportunity to add refereed comments to each protocol. Links in the online protocols offer additional resources and step-bystep instructions print out in a convenient form, complete with materials, cautions, and troubleshooting advice. Each protocol is citable, presented, and edited in the style that has made Molecular Cloning, Antibodies, Cells, and many other Cold Spring Harbor manuals essential to the work of scientists worldwide. The current collection of more than 1000 protocols is continuously expanded, updated, and annotated by the originators and users of the techniques. NEW to CSH Protocols: Emerging Model Organisms, a full-fledged guide to the use of new model systems in the laboratory, covering husbandry, genetics, genomics and basic protocols. CSH Protocols is created by Cold Spring Harbor Laboratory Press in association with HighWire Press of Stanford University. Executive Editor: Dr. David Crotty ISSN / online, monthly Available exclusively via institutional site license For pricing information or to request a free trial, contact us at: Phone: (Continental US and Canada) or (all other locations) Fax: cshpress@cshl.edu Website: Write: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY

6 Gordon Research Conferences For over 75 years, GRC's high-quality, cost-effective meetings have been recognized as the world's premier scientific conferences, where leading investigators from around the globe discuss their latest work and future challenges in a uniquely informal, interactive format. If you can attend only one meeting in 2009, why not visit the Frontiers of Science? Apply to a GRC now and see why attendees consistently rate them "the best conference I've attended this year". Apoptotic Cell Recognition & Clearance Worm-Fly-Vertebrate-Human Clearance & Failure June 28 - July 3, 2009 Colby-Sawyer College, New London, NH Hormone Action In Development & Cancer Hormonal Regulation Of Development & Cancer: Common Signaling Pathways July 26-31, 2009 Holderness School, Holderness, NH CAG Triplet Repeat Disorders (Gordon-Kenan Research Seminar) May 30-31, 2009 Waterville Valley Resort, Waterville Valley, NH -and- CAG Triplet Repeat Disorders May 31 - June 5, 2009 Waterville Valley Resort, Waterville Valley, NH Nucleic Acids May 31 - June 5, 2009 University of New England, Biddeford, ME Nucleosides, Nucleotides & Oligonucleotides July 5-10, 2009 Salve Regina University, Newport, RI Cell Growth & Proliferation July 5-10, 2009 Colby College, Waterville, ME Developmental Biology June 21-26, 2009 Proctor Academy, Andover, NH Human Genetics & Genomics July 19-24, 2009 University of New England, Biddeford, ME Phosphorylation & G-Protein Mediated Signaling Networks June 7-12, 2009 University of New England, Biddeford, ME Stem Cells & Cancer Molecular Mechanisms Controlling Normal And Cancer Stem Cells September 13-18, 2009 Les Diablerets Conference Center, Les Diablerets, Switzerland For program information, fees and site/travel information, please visit our web site: