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1 G r a s p t h e P r o t e o m e Plant Cell Lysis Is your plant cell lysis leafing you out in the cold? The P-PER Reagent Kit (#89803) extracts maximum active protein from stem, root, seed and leaves in 10 minutes without liquid nitrogen. You can trust the experts at to know how to get maximum protein extraction from your sample. We have been the protein people for over 50 years, providing the best solutions to extract, purify and quantify proteins from many types of cell lines and tissue. Leaf Seed* MW (kd) Tobacco Corn Leaves Arabidopsis Soybean Corn Kernel P-PER Reagent Kit Highlights: Convenient disrupts cells without harsh mechanical methods in 10 minutes Compatible downstream applications include 1-D and 2-D gel electrophoresis, Western blotting, activity assays, and protein affinity purifications Quantifiable use the BCA Protein Assay Kit, Reducing Agent Compatible Ready-to-use extract does not require filtration through cheesecloth or Miracloth Provides active proteins extracted proteins are functional New! Poppers Cell Lysis Solutions Handbook Log on to our web site or call us to request your FREE handbook (# ). Outside the U.S., contact Perbio Science or your local distributor Protein extracted (mg)/ total tissue weight (mg) Competitor S Competitor S Competitor S Fresh leaf tissue and seed were lysed and extracted according to the P-PER Kit (Product # 89803) protocol, a competitor s protocol and a literature-based (home brew) protocol. Samples were normalized (weight tissue/volume extract), resolved on a 10% Bis-Tris gel and stained with Imperial Protein Stain (Product # 24615). Samples were also quantified using the BCA Protein Assay Kit, Reducing Agent Compatible (Product # 23250). *The Competitor S kit is not recommended for dried seed. Belgium & Dist.: Tel euromarketing@perbio.com Tel: or Fax: Customer Assistance CS@piercenet.com Outside the United States, visit our web site or call to locate your local Perbio Science branch office (below) or distributor France: Germany: Hong Kong: The Netherlands: Tel Tel Tel Tel euromarketing@perbio.com de.info@perbio.com SalesHK@perbio.com euromarketing@perbio.com China: Tel support@perbio.com.cn United Kingdom: Tel uk.info@perbio.com Biotechnology, Inc., products are supplied for laboratory or manufacturing applications only. BCA, P-PER and Imperial are trademarks of Biotechnology, Inc. U.S. patents pending on P-PER Technology, Imperial Protein Stain and Reducing Agent-compatible BCA Technology. Switzerland: Tel euromarketing@perbio.com

You can trust the experts at to know how to get maximum protein extraction from your sample.

2 ANNOUNCEMENT Plant Physiology Adopts Incentives for Concise Articles Beginning with submissions on January 1, 2006, Plant Physiology is establishing a limit of 10 journal pages for research articles. While longer papers are permitted at a higher page charge, we believe that the 10-page target is a positive incentive for tight composition, reduced repetition, and appropriate use of supplemental data files. Beginning with submissions on January 1, page charges are $75 per page for the first 10 pages ($55 per page if the corresponding author is a member of ASPB), and the charges for the 11th page and above are doubled. An online calculator is available from the Instructions for Authors ( misc/ifora.shtml) and in the submission system that will provide an estimated final page count for your article, should it be accepted. For more information, please see the Editorial in the December 2005 issue ( Cite Track Alerts Cite Track Alerts is a free service providing -based alerts when new articles matching your search criteria are published in Plant Physiology Online, or when designated Plant Physiology Online articles are cited by new articles from a designated set of journals ( help/ijlinks.dtl). Sign Up Now!!!

3 The Arabidopsis Book The American Society of Plant Biologists is pleased to publish The Arabidopsis Book (TAB), a dynamic, fully electronic compilation of chapters co-edited by Chris Somerville, Elliot Meyerowitz, Jeff Dangl, and Mark Stitt and available free of charge on the Internet. TAB offers a new model for scientific publishing. Each of the chapters in the book reviews in detail an important aspect of the plant Arabidopsis thaliana, and the content continually evolves as new information becomes available, making TAB the most comprehensive and current work on Arabidopsis. ASPB is providing funds for the posting and maintenance of TAB on the Internet as a public service. All chapters and updates are hosted in partnership with BioOne ( in both HTML and PDF formats.

Each of the chapters in the book reviews in detail an important aspect of the plant Arabidopsis thaliana, and the content continually evolves as new information becomes available, making TAB the most

4 Plant Physiology Announces a Reactive Oxygen Species Special Issue Plant Physiology is planning a special issue devoted to Reactive Oxygen Species in June All excellent papers covering reactive oxygen species signaling and metabolism, oxidative stress homeostasis, and redox signaling research in plants will be considered. Authors interested in contributing to this special issue should indicate this in their cover letter when submitting papers online at Manuscripts to be considered for the special issue should be submitted no later than January 31, For additional information, please contact Editor-in-Chief Donald Ort (d-ort@uiuc.edu) or Special Issue Editors Julia Bailey-Serres (serres@ucr.edu) and Ron Mittler (ronm@unr.edu). etocs Plant Physiology's FREE etoc service allows anyone who registers to be notified via when new content goes online. You may choose to receive any or all of the following: Notification that a new issue of Plant Physiology is online Planned tables of contents for future issues Complete table of contents for new issues Special Announcements from ASPB SIGN UP NOW!!!

Authors interested in contributing to this special issue should indicate this in their cover letter when submitting papers online at http://submit.plantphysiol.org/.

5 Data That Speaks Volumes The LI-6400 is the most referenced photosynthesis system in scientific literature, and for good reason. Used worldwide for plant research, the LI-6400 provides unmatched integration and proven ruggedness and reliability. For more information on the LI-6400, including a product tour, click on LI-COR Biosciences 4421 Superior Street Box 4425 Lincoln, Nebraska USA North America: International: Fax envsales@licor.com LI-COR GmbH (Germany) +49 (0)

For more information on the LI-6400, including a product tour, click on www.licor.

6 Plant Biology Joint Annual Meeting of the American Society of Plant Biologists and the Canadian Society of Plant Physiologists Société Canadienne de Physiologie Végétale Hynes Convention Center Boston, Massachusetts August 5-9, 2006 SYMPOSIA Plants Mitigating Global Change Stephen P. Long, University of Illinois Legumes: Genomes to Biology Douglas R. Cook, University of California-Davis Ion Channels and Cellular Signaling Julian I. Schroeder, University of California-San Diego Gibbs Medal Symposium: Genome Scale Biology Joseph R. Ecker, The Salk Institute President's Symposium: Plant Responses to the Environment Michael F. Thomashow, Michigan State University For more information: American Society of Plant Biologists Telephone: Fax: Web Site: Photo Credit: Greater Boston Convention & Visitors Bureau

Cook, University of California-Davis Ion Channels and Cellular Signaling Julian I. Schroeder, University of California-San Diego Gibbs Medal Symposium: Genome Scale Biology Joseph R.

Biochemistry & Molecular Biology of Plants

Biochemistry & Molecular Biology of Plants Add Color! Free online color is available for all papers submitted to The Plant Cell. This new feature allows you to have figures appear in color in the online version of your article but in black and