HPLC Flash TLC GC SPE

Transcription

1 Chromatography Essentials HPLC Flash TLC GC SPE

2 Table of Contents HPLC Columns 5 HPLC Accessories 6 30 Flash Products 3 34 TLC Products GC Columns GC Accessories 46 5 SPE and Filtration Equipment Syringes Tubing 7 75 Vials 76 79

3 hplc columns & accessories Grace HPLC Column Solutions Address Today s Separation Challenges hplc columns This catalog features Grace s leading HPLC column technologies. You will find innovative chemistries, unsurpassed reliability, and hardware configurations that optimize your method requirements for speed, sensitivity, resolution or sample loading. Discover how our select range of small molecule columns, biomolecule-based offerings and ion chromatography technologies address your toughest separation challenges. Grace Featured Column Families: VisionHT Unique chemistries in sub to 0µm particles maximize performance and speed for UHPLC and HPLC. Everest High capacity for complex peptide mapping and proteomics applications. Alltima HP Premium quality, exceptionally stable columns, without phase bleed. ProZap High-speed protein and peptide separations. Prevail Compatible with 00% aqueous to 00% organic mobile phases. Vydac TP Popular, industry-standard bioseparations columns. GraceSmart High quality HPLC columns at an exceptional value. Alltech IC Wide variety for the separation of organic and inorganic anions and cations. Vydac MS Unique selectivity and recovery for protein isolation and characterization. Grace Expertise and Product Ownership Grace has been manufacturing silica and bonding it with unique chemistries for more than three decades. Our combined media production, column packing, and subμm particle expertise uniquely positions us to solve the challenges of modern chromatography laboratories. Partner with Grace and be assured our consistent, reliable chromatography products adhere to strict quality standards. Additional Column Brands Available While this catalog focuses on Grace leading HPLC columns, other popular brands are still available. Visit our website for a comprehensive listing. Alltima Adsorbosphere Genesis Grom Sil Certified in ISO 900, 4000 and 3485 procedures FDA CFR 80 for medical devices Full traceability from base silica to final product Extensive process control to ensure highest reproducibility Experienced global technical support and sales teams Allsphere Apex Apollo Brava Denali Econosphere Grom Sapphire Macrosphere Platinum ProSphere Vydac SP

4 hplc columns & accessories HPLC Column Hardware Formats HPLC Column Hardware Formats i.d. Format (mm) UHPLC Capillary Length (mm) System Compatibility Advantage Brand Availability VisionHT UHPLC system, LC/MS Capillary systems, LC/MS Ultra-low dead volume hardware, pressure rated to 8,000psig, allows 7X faster linear velocities. Highly specialized columns packed in small diameter fused silica tubing. Packed with subµm media, this hardware delivers the fastest separations with maximum efficiency. Extremely low sample dispersion offers best sensitivity. High-sensitivity and samplelimited applications. Alltima HP Vydac MS Everest hplc columns Expedite Low volume, highthroughput (HTP) systems Extremely short, low-volume columns that prevent sample diffusion. High-throughput and rapid resolution LC/MS applications packed with highly efficient.5µm media. VisionHT Alltima HP Prevail ProZap Microbore Standard HPLC optimized for low volume.mm diameter analytical columns requiring low volume systems. Low solvent consumption and improved sensitivity compared to traditional 4.6mm columns. VisionHT Alltima HP Prevail GraceSmart Vydac MS Everest IC Solvent Reducer Standard HPLC 3mm diameter analytical columns suitable for use on conventional HPLC. 50% solvent savings compared to traditional 4.6mm columns. No need to optimize system for low volume. VisionHT Alltima HP Prevail Vydac MS Vydac TP Analytical Standard HPLC Conventional 4.6mm i.d. with industry standard port configurations. Most common, traditional hardware format. VisionHT Alltima HP Prevail GraceSmart Vydac MS Everest Vydac TP IC Rocket Standard HPLC Large 7mm i.d. balances column volume with conventional LC system volume, and allows faster mobile phase flow rates. Ultra-fast separations on conventional HPLC systems. Unique hardware geometry reduces peak broadening and delivers excellent peak shapes. 5mL/min max flow rate reduces analysis time up to 80% compared to traditional analytical columns. VisionHT Alltima HP Prevail Preparative Preparative LC Large i.d. increases loading. Packed under high pressure for high efficiency LC purifications. VisionHT Vydac MS Vydac TP Capillary Guard UHPLC Guard 0 Guard system with reusable holder and disposable cartridges. Protects capillary columns and prolongs life. 5 Integral and stand-alone options. Protects UHPLC column investment, and prolongs life. Alltima HP Vydac MS Everest VisionHT All-Guard Guard system with reusable holder and disposable cartridges. Protects analytical and microbore columns and prolongs life. VisionHT Alltima HP Prevail Vydac MS Everest Vydac TP 3

5 hplc columns & accessories HPLC Phase Specifications and Usage hplc columns Columns for Small Molecules Brand VisionHT Phase C8 HighLoad Pore Size (Å) Surface Area (m /g) Carbon Load (%) Endcapped Chromatographic Properties Application/Benefit 0 0 Yes Ultra high-purity silica, fully bonded. C8 Basic Proprietary Ultra-high purity silica. Controlled silica surface exposure gives dual mode separation with polar and nonpolar analytes. C8 Classic Yes Lower carbon load. Slight silica exposure. C8 Polar No High silica exposure, low carbon load. Uniform coverage of inert vicinal silanols. HILIC 0 0 No Polar phase with shorter equilibration times. Shipped in ACN/Water. Silica 0 0 No Traditional normal phase for use in 00% organic mobile phases. General purpose for broad range compounds, classic selectivity, high-capacity for hydrophobic compounds. Alternate reversed-phase selectivity. High polar retention especially with compounds having two or more polar groups. Excellent sensitivity and peak shape for basic compounds, without the need for acidified mobile phases. Reversed-phase separations with reduced bonding optimized for speed. Some additional polar retention. Unique polar selectivity. Low carbon load gives fastest reversed-phase elution times while retaining polar compounds longer. Peak reversal compared to reversed-phase. Ideal for very polar compounds with high organic mobile phases for improved sensitivity by MS. For isomeric separation of non-aqueous compatible compounds by absorption chromatography. Alltima HP C Yes Classic reversed-phase retention and selectivity. Routine applications. C8 EPS Yes Greater retention and enhanced Reversed-phase applications where C8 is too peak symmetry for polar retentive. compounds. Alternate selectivity to traditional reversed-phases. C8 Hi-Load Yes Highest carbon load for superior High resolution for complex samples. retention and loadability. C8 AQ Yes 00% water wettable. Applications requiring high aqueous mobile phases. C8 Amide Yes Polar-embedded phase with extremely low bleed. Compatible with microbore. C Yes Lower capacity compared to C8 phases. Cyano Yes Extremely stable, long life and reproducible. Basic compounds in neutral to alkaline ph, MS applications. Reversed-phase applications where C8 is too retentive. Ideal for basic drug analysis. Silica No Highly polar phase. General purpose normal phase applications. L3 HILIC 0 30 No Hydrophilic interaction Very polar analytes that cannot be retained by L3 chromatography uses small amounts of water for increased sensitivity with microbore applications. reversed-phase. Prevail C8 Select Yes Stable in 00% aqueous to 00% organic mobile phases. Same benefits as Prevail C8, but less retentive for polar compounds. L C Yes Stable in 00% aqueous to 00% organic mobile phases. Flexibility to switch between varied mobile phase conditions to suit a variety of applications. Excellent sensitivity for microbore applications. C Yes Stable C8 phase. Use for highly hydrophobic compounds that L7 retain too strongly on C8. Phenyl Yes Lowest hydrophobic capacity. Selective for aromatic compounds in a variety of L mobile phase conditions. Cyano Yes General purpose cyano suitable Rugged normal phase applications. L0 for normal or reversed-phase use. Amino No Stable in 00% aqueous to Use for carbohydrates or as a weak anion L8 00% organic mobile phases. exchanger. Silica No Highly polar phase. General purpose normal phase applications. L3 Organic Acid Yes Highly efficient silica-based, Separate common organic acids with acid-stable. unsurpassed resolution, speed and sensitivity. Lower cost than polymeric columns. Carbohydrate No Extremely stable hybrid phase. Excellent for mono- and oligosaccharides and ES sugar alcohols. GraceSmart C Yes High purity silica with monomeric C8 bonding offers classical reversed-phase selectivity. Excellent value for general purpose, routine applications. USP L-code L L L L L3 L3 L L L L L L7 L0 L L 4

6 hplc columns & accessories Columns for Large Molecules / Life Science Pore Surface Carbon Brand Phase Size (Å) Area (m /g) Load (%) Endcapped Chromatographic Properties Application/Benefit Vydac MS 8MS C Yes Polymeric bonding, highest hydrophobic Use for simple enzymatic digests (< proteins) interaction and unique geometric selectivity. or biomolecules 0 5K MW. 38MS C Yes Monomeric bonding offers increased peptide interaction and generally yields higher peak counts. 08MS C Yes Lower hydrophobicity is better for larger biomolecules. 4MS C Yes Lower capacity than C8 or C8, suitable for hydrophobic proteins or when minimal organic is desired. 9MS Yes Lowest capacity, aromatic functional Diphenyl groups. Everest 38EV C Yes Maximum surface coverage for highest resolution of complex samples. Use for same applications as 8MS, but offers different C8 selectivity. Ideal for biomolecules 5 0K MW. Ideal for biomolecules >0K MW, undenatured intact proteins, antibodies, oligonucleotides, human growth hormone. Highly selective for proteins with aromatic side chains. Complex enzymatic digests (> proteins). ProZap C Yes Subµm, 500Å wide pore. Ideal for fast, intact protein or peptide analysis. L Vydac TP 8TP C Yes First generation polymeric C8 media with Small polypeptides 4 5K MW, enzymatic digest L unique selectivity. fragments, natural and synthetic peptides, multi-ring compounds. 38TP C Yes First generation monomeric C8 media. Use for same applications at 8TP, but offers L different C8 selectivity. 08TP C Yes Less hydrophobic than C8TP media. Polypeptides 0 0K MW. L7 4TP C Yes First generation C4 media. Glycoproteins, hemoglobin variants, histones, insulin variants, membrane proteins. L6 9TP Diphenyl Yes Lowest capacity first generation diphenyl media. Reversed-Phase Column Comparisons Polypeptides with aromatic side chains, large hydrophobic proteins, membrane-spanning peptides, lipid peptides, fusion proteins from inclusion bodies. USP L-code L Based on the widely accepted work and test procedures of Drs Lloyd Snyder and John Dolan,, 3, Grace developed this tool to show relative selectivity comparisons for commercially available columns. This graph plots the indicators for hydrophobicity versus the cation exchange capacity at ph 7. Hydrophobicity is often the primary analyte interaction and indicates overall capacity. The cation exchange capacity measures secondary interactions that take place and can greatly influence the retention of polar analytes. L L7 L6 L L L hplc columns VisionHT C8 Polar VisionHT C8 Basic Acquity HSS C8 SB Extended Polar Retention Prevail Amide Prevail C8 Universally Retentive Increasing Polar Retention Alltima HP C8 Amide Alltima HP C8 EPS Synergi Polar-RP Zorbax SB-AQ To find columns that will produce alternate separation profiles, choose points that are in opposite quadrants. Prevail Select C8 GraceSmart C8 Atlantis T3 VisionHT C8 Classic Hypersil GOLD aq Hypersil GOLD Acquity BEH Shield RP-8 Kinetex XB-C8 Gemini C8 Acquity HSS T3 Kinetex C8 Ascentis ACE 5 C8 RP-Amide XTerra MS C8 Luna C8 Zorbax SB C8 To find columns with similar selectivity, choose points that are close together. Inertsil ODS- Zorbax Rx-8 Hypersil ODS- Hypersil BDS C8 Pinnacle DB C8 Alltima HP C8 VisionHT C8 HighLoad XBridge C8 Symmetry C8 Zorbax Eclipse XDB-C8 Acquity HSS C8 Zorbax Extend C8 Kromasil 00 5 C8 XTerra C8 RP Alltima HP C8 High Load Low Capacity Increasing Hydrophobic Retention Traditional RP Selectivity Data courtesy of Lloyd Snyder and John Dolan. The Hydrophobic-subtraction Model of Reversed-phase Column Selectivity, L.R. Snyder, J.W. Dolan and P.W. Carr, J. Chromatogr. A, 060 (004) A New Look at the Selectivity of Reversed-phase HPLC Columns, L.R. Snyder, J.W. Dolan and P.W. Carr, Anal. Chem., 79 (007)

7 hplc columns & accessories Application-Based Column Selection Use this chart to identify a suitable column for your application. Traditional choice columns are well matched to the application. Alternative choice columns can provide different selectivity when the traditional choice column does not work. Legend: Traditional Choice hplc columns Application-Based Column Selection Pharmaceutical Neutraceutical / Natural Products Food & Beverage Environmental Biopharmaceutical/ Life Science Acidic Column VisionHT C8 HighLoad VisionHT C8 Basic VisionHT C8 Classic VisionHT C8 Polar VisionHT Silica Alltima HP C8 Alltima HP C8 EPS Alltima HP C8 Hi-Load Alltima HP C8 AQ Alltima HP C8 Amide Alltima HP C8 Cyano Prevail C8 Select Prevail C8 Prevail Phenyl Prevail Organic Acid Prevail Carbohydrate ES GraceSmart C8 Vydac 8MS C8 Vydac 38MS C8 Vydac 08MS C8 Vydac 4MS C4 Vydac 9MS Diphenyl Everest 38EV C8 ProZap C8 Vydac 8TP C8 Vydac 4TP C4 *For UHPLC applications. Basic Neutral Herbal Medicines Vitamins Dietary Supplement Carbohydrates Lipids Organic Acids Food Safety Agricultural Chemicals Industrial/Aromatics Simple Enzymatic Digests (< proteins) Complex Enzymatic Digests (> proteins) Proteins, Peptides, Biomolecules 0 5K MW * * * * * * Alternative Choice 5 0K MW >0K MW Undenatured, Intact Proteins Antibodies Oligonucleotides 6

8 hplc columns & accessories Empty HPLC Hardware Stainless Steel Analytical Column Hardware Stainless Steel Preparative Column Hardware Threaded Column Tube Frit/Insert Endfitting Column Tube Continuously Adjustable Piston/Frit Endfitting hplc columns Stainless steel analytical columns come complete with the threaded tube, two µm industry standard frit/inserts, and two stainless steel endfitting nuts. Optional 0.5µm porosity frit/ inserts are also available. Adapters and sealing rings are available for slurry packing. The packing adapter comes with a /4" precolumn tube to connect to /4" slurry reservoirs. Stainless Steel Analytical Column Hardware Specifications Material: 36 Stainless Steel Max. Temperature: 00ºC Max. Pressure: 0,000psig Thread Type: 0-3 UNF (CPI Standard Ports) Typical Use: Analytical HPLC Columns Stainless Steel Analytical Hardware and Accessories.mm i.d. 4.6mm i.d. Complete Columns 50mm mm mm mm mm 6558 Replacement Parts Endfitting (ea) Frit/Insert, µm (5/pk) Frit/Insert, 0.5µm (5/pk) 750 The easy-to-use preparative format has threaded endfittings and a continuously adjustable piston/frit. The piston/frit extends column life by compressing the packed bed to remove voids. Stainless Steel Preparative Column Hardware Specifications Material: 36 Stainless Steel, PEEK Max. Temperature: 00ºC Max. Pressure: 8000psig Thread Type: 0-3 UNF (CPI Standard Ports) Typical Use: Prep HPLC Columns Stainless Steel Preparative Hardware and Accessories i.d. Length Qty. Complete Columns 7mm 00mm ea mm 50mm ea mm 00mm ea mm 50mm ea 965 mm 70mm ea 9654 mm 00mm ea 9650 mm 50mm ea 965 mm 500mm ea 965 Replacement Piston/Frit, µm 7mm ea mm ea 9657 mm ea 9657 Stainless Steel Frits Stainless Steel Frit Specifications Material: 36 Stainless Steel Max. Temperature: 500ºC Max. Pressure: 0,000psig Typical Use: Column Inlet/Outlet Stainless Steel Frits Frit Diameter Total Diameter Thickness Porosity 5/pk /4" (6.350mm) /4" (6.350mm) 0.03" (0.79mm) 0.5µm 7085 /4" (6.350mm) /4"(6.350mm) 0.03" (0.79mm).0µm 705 /4" (6.350mm) /4" (6.350mm) 0.063" (.59mm).0µm /8" (9.55mm) 3/8" (9.55mm) 0.03" (0.79mm).0µm 745 Encased Metal-Free Frits Encased Metal-Free PEEK Frit Specifications Material: PEEK Max. Temperature: 00ºC Max. Pressure: 0,000psig Typical Use: Column Inlet/Outlet Encased Metal-Free PEEK Frits Frit Diameter Total Diameter Thickness Porosity 5/pk PEEK Frits with PEEK Ring 0.8" (4.6mm) /4" (6.4mm) 0.063" (.59mm) 5µm " (4.6mm) /4" (6.4mm) 0.063" (.59mm) 0µm 6907 technical assistance Contact Tech Support: [email protected] Online: 7

9 hplc columns & accessories hplc columns VisionHT Columns One Platform for UHPLC, HPLC and PREP Easy method transfer between UHPLC, HPLC and preparative chromatography Broad selectivity range unique complementary phase chemistries Maximize speed and resolution with sub μm on any LC system Separate complex samples with excellent resolution with VisionHT high performance columns. They are available with.5, 3, 5 and 0μm particle sizes and six different phase selectivities spanning the full polarity spectrum. The same base silica and bonding chemistries are used across all particle sizes therefore offering simple method transfer between UHPLC, HPLC and preparative systems. VisionHT Phase Specifications Pore Size (Å) Surface Area (m /g) Carbon Load (%) Endcapped Chromatographic Properties Phase C8 HighLoad 0 0 Yes Ultra-high purity silica, fully bonded. C8 Basic Proprietary Ultra-high purity silica. Controlled silica surface exposure gives dual mode separation with polar and non-polar analytes. C8 Classic Yes Lower carbon load. Slight silica exposure. C8 Polar No High silica exposure, low carbon load. Uniform coverage of inert vicinal silanols. HILIC 0 0 No Polar phase with shorter equilibration times. Shipped in ACN/Water. Silica 0 0 No Traditional normal phase for use in 00% organic mobile phases. Application/ Benefit General purpose for broad range compounds, classic selectivity, high-capacity for hydrophobic compounds. Alternate reversed-phase selectivity. High polar retention especially with compounds having two or more polar groups. Excellent sensitivity and peak shape for basic compounds, without the need for acidified mobile phases. Reversed-phase separations with reduced bonding optimized for speed. Some additional polar retention. Unique polar selectivity. Low carbon load gives fastest reversed-phase elution times while retaining polar compounds longer. Peak reversal compared to reversed-phase. Ideal for very polar compounds with high organic mobile phases for improved sensitivity by MS. For isomeric separation of non-aqueous compatible compounds by absorption chromatography. USP L-code L L L L L3 L3 Simple Method Transfer Optimizing and transferring methods between HPLC, UHPLC and preparative systems is not a simple and intuitive task. However, when the same base silica and bonding is available in sub-, 3, 5, and 0μm particles and in a variety of column formats, methods can be more easily transferred across system types and between laboratories, improving efficiency and productivity. Identical Selectivity Across All Particle Sizes Snyder-Dolan-Carr Coefficients for VisionHT C8 Basic Phase.5µm 3.0µm 5.0µm 0µm H S A B C.8 C7 References:. "The "Hydrophobic-subtraction" Model of Reversed-phase Column Selectivity", L.R. Snyder, J.W. Dolan and P.W. Carr, J. Chromatogr. A, 060 (004) "A New Look at the Selectivity of Reversed-phase HPLC Columns", L.R. Snyder, J.W. Dolan and P.W. Carr, Anal. Chem., 79 (007) Method Transfer x 50mm, 6 5µm,.mL/min. 5. Uracil. Benzyl alcoho 3. Benzonitrile 4. Anisole 5. Ethyl benzene 6. Hexaphenone 4.6 x 50mm, 3µm,.mL/min. 7 x 53mm, 3µm,.0mL/min.. x 50mm,.5µm, 0.mL/min. 7 x 53mm, 3µm, 5.0mL/min min. Column: VisionHT C8, HighLoad, Dimensions noted Mobile Phase: A: 30mm phosphate buffer, ph7 B: Acetonitrile Gradient: Time: %B:

10 hplc columns & accessories Broad Selectivity Range Four reversed-phase column chemistries offer complementary selectivity. The unique VisionHT C8 Polar and Basic phases retain polar compounds while the C8 HighLoad has a more traditional reversed-phase selectivity. Using phases with orthogonal selectivity gives confidence that if one phase does not produce the separation the other will. VisionHT Reversed-Phases Span the Full Polarity Range VisionHT C8 Polar Phase VisionHT C8 VisionHT Basic C8 Column Basic Strongest retention for polar compounds Key VisionHT columns Commercially available columns hplc columns Increasing Polar Retention VisionHT C8 Classic Column Classic retention for hydrophobic compounds VisionHT C8 HighLoad High-Load Column Increasing Hydrophobic Retention References:. "The "Hydrophobic-subtraction" Model of Reversed-phase Column Selectivity", L.R. Snyder, J.W. Dolan and P.W. Carr, J. Chromatogr. A, 060 (004) "A New Look at the Selectivity of Reversed-phase HPLC Columns", L.R. Snyder, J.W. Dolan and P.W. Carr, Anal. Chem., 79 (007) Different Selectivities of VisionHT Phases, Neutral Compounds. Benzonitrile. Anisole 3. 5,5-Diphenylhydantion 4. 5-Methyl salicylaldehyde VisionHT Classic VisionHT HighLoad VisionHT Basic VisionHT Polar min. Column: Various VisionHT Phases,.5µm,.0 x 50mm Mobile Phase: A: ACN B: Water Gradient: Time: 0 %B: 90 Flow Rate: 0.mL/min Detector: UV 0nm Different Selectivities of VisionHT Phases, Polar Compounds Acetaminophen. Caffeine 3. N,N'-Diethylacetamide 4. Benzonitrile min. Column: Various VisionHT Phases,.5µm,.0 x 50mm Mobile Phase: A: ACN B: Water Gradient: Time: 0 %B: 90 Flow Rate: 0.mL/min Detector: UV 0nm 4 VisionHT Classic VisionHT HighLoad VisionHT Polar VisionHT Basic 9

11 hplc columns & accessories Consistent and Reliable Performance Ultra-high mechanical strength silica and robust bonding chemistry minimize variations in capacity and selectivity. Media consistency combined with reproducible column packing methods delivers reliable performance and long column lifetime. hplc columns Reproducible Particle Technology Surface Area Mean = 07 RSD =.6% Pore Volume Mean = 0.5 RSD = 0.64% Pore Diameter Mean = 99.6 RSD =.5% 50 Consistent Column-to-Column Performance Biphenyl Nitrobenzene Amitriptyline Cinnamic Acid Toluene 6.00 Surface Area (m /g) Pore Diameter (Å) Pore Volume (cc/g) Capacity Factor Consecutive Batches Consecutive Batches VisionHT silica's surface area, pore volume, and pore diameter are highly reproducible. VisionHT media and advance packing methods produce columns with highly consistent capacity and selectivity. 0 0 Constant Performance after Routine Exposure to 6,000psig Pressure min. UHPLC columns rated to 6,000psig pressure limit Uracil (0.05mg/mL). Phenol (0.7mg/mL) 3. N,N-Diethyl-m-toluamide (0.6mg/mL) 4. Toluene (4.0mg/mL) INITIAL 3 4 min. 0 FINAL (After 0,000 column volumes at 6,000psig) Plates/Meter High-Pressure Stability Column Comparison, x 50mm* VisionHT Column VisionHT Column 5 VisionHT Column VisionHT Column 6 VisionHT Column 3 VisionHT Column 7 VisionHT Column 4 Competitor Sub µm Column Column Volumes Competitor Sub µm Column Column: Mobile Phase: Flow Rate: Detector: VisionHT C8 Polar,.5μm,.0 x 00mm ( 53960) (58:4) Acetonitrile:Water 0.mL/min UV at 0nm *After exposure to,000psig Before and after chromatograms show a consistent level of performance after exposure to 6,000psig pressures for 0,000 column volumes. Competitive columns lose performance over time under high-pressure conditions while VisionHT columns remain stable. 0

12 hplc columns & accessories Method Development Ultra MD Kits Experience Broad Selectivity Range of the VisionHT Phases Ultra MD kits include 4 phases with orthogonal selectivity to enable fast and efficient method development for the separation of very polar to very hydrophobic compounds. VisionHT Column Ultra MD Kits Phases Dimensions Ultra MD Kit C8 Classic, C8 Polar, x 00mm 5469 C8 HighLoad, C8 Basic Ultra MD Kit C8 Classic, C8 Polar, x 50mm C8 HighLoad, C8 Basic Ultra MD Kit 3 C8 Classic, C8 Polar, C8 HighLoad, C8 Basic x 50mm 5469 VisionHT Guard Columns Protect Your Column to Minimize Downtime and Reduce Cost Couple the zero-dead volume integral guard directly to any VisionHT column, with no loss in efficiency. hplc columns VisionHT Columns i.d. (mm) Length (mm) Particle Size (µm) C8 HighLoad C8 Basic C8 Classic C8 Polar HILIC Silica Format UHPLC Expedite Microbore Solvent Reducer Analytical Rocket Preparative Capillary Guard* All-Guard 3pk** *Requires Capillary holder, **Requires All-Guard Holder, 800. more info For detailed information on column formats, see page 3. technical assistance Contact Tech Support: [email protected] Online:

13 hplc columns & accessories hplc columns Alltima HP Columns Stability, Purity and Performance Better peak symmetry high-purity silica eliminates peak tailing problems Long column life exceptional column stability minimizes downtime and reduces cost Ideal for microbore or critical analysis low to no detectable column bleed Multiple selectivity options optimizes retention, resolution and analysis time Alltima HP columns combine our best phase chemistries with high-purity silica. The result is one product family with the selectivity and performance needed to help overcome your most challenging separation needs. Alltima HP Phase Specifications Phase Pore Size (Å) Surface Area (m /g) Carbon Load (%) Endcapped Chromatographic Properties C Yes Classic reversed-phase retention and selectivity. Application/ Benefit Routine applications. C8 EPS Yes Greater retention and enhanced peak Reversed-phase applications symmetry for polar compounds. Alternate where C8 is too retentive. selectivity to traditional reversed-phases. C8 Hi-Load Yes Highest carbon load for superior retention High resolution for complex and loadability. samples. C8 AQ Yes 00% water wettable. Applications requiring high aqueous mobile phases. C8 Amide Yes Polar-embedded phase with extremely Basic compounds in neutral to low bleed. Compatible with microbore. alkaline ph, MS applications. C Yes Lower capacity compared to C8 phases. Reversed-phase applications where C8 is too retentive. Cyano Yes Extremely stable, long life and Ideal for basic drug analysis. reproducible. Silica No Highly polar phase. General purpose normal phase applications. HILIC 0 30 No Hydrophilic interaction chromatography uses small amounts of water for increased sensitivity with microbore applications. Very polar analytes that cannot be retained by reversed-phase. USP L-code L L L L L L7 L0 L3 L3 The Alltima HP Column Family Demonstrates Low Bleed Alltima HP columns are among the lowest-bleed columns available, making them ideal for microbore applications. The C8 amide polar-embedded phase and the cyano phase have significantly lower column bleed than competitive columns. Column Bleed by ESI-MS Alltima HP vs. Competitive Columns Alltima HP Cyano Much More Stable than Other Cyanos Relative Abundance Level (X0 5 ) No Column 0.8 Alltech Alltima HP Column Competitor Columns Alltima HP Silica Alltima HP C8 Alltima HP C8 Hi-Load C8 MS C8 MS.9 Alltima HP C8 Amide.5.8 C8 Amide 3. C8 Amide % Changes in Retention ph.0 ph 7.5 Alltima HP CN Brand A CN Brand B CN Brand C CN Brand D CN Brand E CN Brand F CN QC D4 Test: 70:30 HO/CH3CN,.0mL/min, UV at 54nm, 5µL D4Mix, Column 4.6 x 50mm, C ph.0 Stability: Flushed with HSO4 ph.0:acetonitrile 50:50 at.0ml/min for 3 days ph 7.5 Stability: Flushed with 0mM KHPO4 ph 7.5:Acetonitrile 50:50 at.0ml/min for 3 days

14 hplc columns & accessories Selectivity Options in One Product Family Capacity Factor k' Alltima HP Column Selectivity Comparisons CN k' Toluene k' Butyl Benzoic Acid k' Amitriptyline C8 EPS C8 C8 Amide C8 C8 AQ C8 Hi-Load Acid, Base and Neutral Test Mix Min. Column: 4.6 x 50mm Mobile Phase: 50mM KHPO4, ph 3.0:CH3CN (40:60) Flow Rate:.0mL/min Detector: UV at 54nm Uracil. Pyridine 3. Phenol 4. N,N-diethyl-m-toluamide 5. 4-Butyl Benzoic Acid 6. Toluene C8 C8 EPS C8 Hi-Load hplc columns Alltima HP Columns i.d. (mm) Length (mm) Particle Size (µm) C8 C8AQ EPS C8 C8 Hi-Load C8 Amide C8 CN Silica HILIC Format Capillary Expedite Microbore Solvent Reducer Analytical Rocket Guard*3pk *Requires All-Guard Holder, 800. more info For detailed information on column formats, see page 3. technical assistance Contact Tech Support: [email protected] Online: 3

15 hplc columns & accessories hplc columns Prevail Columns Stable from 00% Organic to 00% Aqueous Exceptional bonded phase stability for long column life Excellent sensitivity with microbore and ELSD applications Prevail HPLC columns offer exceptional versatility for difficult separations. Traditional reversed-phase packings experience bonded phase collapse under highly aqueous (>95%) conditions. Prevail bonded phases remain fully extended in 00% aqueous and offer a high carbon load for exceptional retention at 00% organic. Retain both polar and nonpolar compounds simply by adjusting the mobile phase. Prevail Phase Specifications Phase Pore Size (Å) Surface Area (m /g) Carbon Load (%) Endcapped Chromatographic Properties Application/ Benefit C8 Select Yes Stable in 00% aqueous to Same benefits as Prevail C8, but slightly 00% organic mobile phases. more retentive. C Yes Stable in 00% aqueous to 00% organic mobile phases. Flexibility to switch between varied mobile phase conditions to suit a variety of applications. Excellent sensitivity for microbore applications. C Yes Stable C8 phase. Use for highly hydrophobic compounds that L7 retain too strongly on C8. Phenyl Yes Lowest hydrophobic capacity. Selective for aromatic compounds in a L variety of mobile phase conditions. Cyano Yes General purpose cyano Rugged normal phase applications. L0 suitable for normal or reversed-phase use. Amino No Stable in 00% aqueous to Use for carbohydrates or as a weak anion L8 00% organic mobile phases. exchanger. Silica No Highly polar phase. General purpose normal phase applications. L3 Organic Acid Yes Highly efficient silica-based, acid-stable. Separate common organic acids with unsurpassed resolution, speed and sensitivity. Lower cost than polymeric columns. Carbohydrate ES No Extremely stable hybrid phase. Excellent for mono- and oligosaccharides and sugar alcohols. USP L-code L L Switch Between 00% Aqueous and 00% Organic Mobile Phases on the Same Column Water-Soluble Vitamins (00% Aqueous) 9465 Fat-Soluble Vitamins and Tocopherols (00% Organic) 9405 Water-Soluble Vitamins (00% Aqueous) Thiamine. Ascorbic Acid 3. Nicotinic Acid 4. Pyridoxine 5. Niacinamide 6. Pantothenic Acid. Vitamin A. δ-tocopherol 3. γ-tocopherol 4. α-tocopherol (Vitamin E) 5. Vitamin D 6. Vitamin D3 7. Vitamin K Thiamine. Ascorbic Acid 3. Nicotinic Acid 4. Pyridoxine 5. Niacinamide 6. Pantothenic Acid wash with intermediate wash with intermediate Min Min Min. Column: Mobile Phase: Flow Rate: Detector: Prevail C8, 5µm, 4.6 x 50mm 5mM KHPO4 ph 3.0:CH3CN (97:3).0mL/min UV at nm Column: Prevail C8, 5µm, 4.6 x 50mm Mobile Phase: CH3CN:CH3OH (75:5) Flow Rate:.5mL/min Detector: UV at 0nm Column: Mobile Phase: Flow Rate: Detector: Prevail C8, 5µm, 4.6 x 50mm 5mM KHPO4 ph 3.0: CH3CN (97:3).0mL/min UV at nm 4

16 hplc columns & accessories Prevail Carbohydrate ES Columns Longer column life hybrid phase has the ruggedness of silica with the stability of a polymer Quieter baselines compared to traditional amino carbohydrate columns, even with gradients Reduce run times with more mobile phase choices Compatible with gradient ELSD applications Quiet Gradient Baselines Prevail Carbohydrate ES columns are excellent for isocratic separations with common detectors, but the column's real power shows with the ELSD and a solvent gradient. Enjoy the powerful selectivity, reduced run times and efficient peaks from gradients while seeing the quiet, stable baselines that maximize sensitivity. Better Resolution and Peak Shape Prevail Carbohydrate ES columns are versatile enough for mono- and oligosaccharides and sugar alcohols. They produce single peaks for reducing sugars at ambient temperatures, eliminating the need for column heating. hplc columns Enhanced Detector Sensitivity Through Flat Gradient Baselines Min Min. Column: Prevail Carbohydrate ES, 5µm, 4.6 x 50mm Mobile Phase: A: Acetonitrile B: Water Gradient: Time: 0 5 %B: Flow Rate:.0mL/min Detector: ELSD. Dextrose. Maltose 3. Maltotriose 4. Maltotetraose 5. Maltopentaose 6. Maltohexaose 7. Maltoheptaose 8. Maltooctaose 9. Maltononaose 0. Maltodecaose. Maltoundecaose. Maltododecaose 3. Maltotridecaose 4. Maltotetradecaose 5. Maltopentadecaose Robitussin Flu Syrup Min.. Sucralose. Fructose 3. Sorbitol 4. Glucose 5. Sucrose 6. Maltose Column: Prevail Carbohydrate ES, 5µm, 4.6 x 50mm Mobile Phase: A: Acetonitrile B: Water Gradient: Time: %B: Flow Rate:.0mL/min Detector: ELSD 0557 Prevail Columns i.d. (mm) Length (mm) Particle Size (µm) C8 C8 Select C8 Phenyl CN Amino Silica Organic Carbohydrate ES Format Expedite Microbore Solvent Reducer Analytical Rocket Guard* *Requires All-Guard Holder,

17 hplc columns & accessories hplc columns GraceSmart Columns High Quality at Exceptional Value High purity phases Efficient and reproducible separations Classic reversed-phase selectivity GraceSmart HPLC phases use high purity silica and are monomerically bonded with uniform coverage. This translates into symmetrical peaks for acids/bases and predictable reversed-phase selectivity. Whether for routine analysis or new method development, use GraceSmart columns to get premium performance at an exceptional value. GraceSmart Phase Specifications Pore Size (Å) Surface Area (m /g) Carbon Load (%) Endcapped Chromatographic Properties Phase C Yes High purity silica with monomeric C8 bonding offers classical reversed-phase selectivity. Application/ Benefit Excellent value for general purpose, routine applications. USP L-code L Consistent and Reproducible Our tightly controlled silica synthesis and bonding keep capacity factor and selectivity variations to a minimum for acidic, basic, and neutral compounds. Competitive Performance GraceSmart columns show similar or better efficiency and asymmetries for challenging base and acid components in comparison to industry leading columns. Capacity Factor Toluene N-Butylbenzoic Acid Anisole Highly Reproducible Batch Acetophenone P-Nitrophenol Amitriptyline Symmetry Plates / Columns,000 0,000 8,000 6,000 4,000, Nicotine Nicotine Efficiency Quinine Symmetry Quinine Diphenhydramine Diphenhydramine Nortriptyline Nortriptyline Competitor C8 Column Competitor C8 Column,4 Dichlorophenoxy Acetic Acid,4 Dichlorophenoxy Acetic Acid,4 Dichlorophenoxy Propionic Acid,4 Dichlorophenoxy Propionic Acid,4 Dichlorophenoxy Butyric Acid,4 Dichlorophenoxy Butyric Acid Competitor 3 C8 Column GraceSmart C8 Column 4 3 Carbamates Min Fenuron. Monuron 3. Fluometuron 4. Diuron 5. Methiocarb 6. Linuron 7. Neburon 3 4 Folium Ginkgo Min Ginkgolide C. Bilobalide 3. Ginkgolide A 4. Ginkgolide B Column: GraceSmart C8, 5µm, 4.6 x 50mm Mobile Phase: A: 0.05M KHPO4, ph 3. B: Acetonitrile Gradient: Time: 0 0 %B: Flow Rate:.0mL/min Detector: UV at 40nm Column: GraceSmart C8, 5µm, 4.6mm x 50 Mobile Phase: Methanol:Tetrahydrofuran:Water (5:0:65) Flow Rate:.0mL/min Detector: ELSD 6

18 hplc columns & accessories mau Column: Mobile Phase: Flow Rate: Detector: Amoxicillin (U.S.P.) Min. GraceSmart C8 Column Luna C8 Column Inertsil ODS-3 Column Hypersil BDS C8 Column Kromasil 00-5 C8 Column 0697 GraceSmart C8, 5µm, 4.6 x 50mm Isocratic, 0.05N Monobasic Potassium Phosphate ph 5:Acetonitrile (96:4).5mL/min UV at 30nm Min. 7 Standards Mix. Nicotine. Quinine 3. Diphenhydramine 4. Nortriptyline 5.,4, Dichlorophenoxy Acetic Acid 6.,4, Dichlorophenoxy Propionic Acid 7.,4, Dichlorophenoxy Butyric Acid Column: GraceSmart C8, 5μm, 4.6 x 50mm Mobile Phase: 50% MeCN/50% 50mM, KHPO4, ph 3 Flow Rate:.0mL/min Detector: UV at 54nm Ibuprofen (U.S.P.) Valerophenone. Ibuprofen hplc columns Hypersil BDS C8 Kromasil 00-5 C8 USP GraceSmart Luna Inertsil Spec C8 C8 ODS-3 N/A Ret. Time (min) Theoretical plates Tailing Factor k'. k' Min. Column: GraceSmart C8, 5µm, 4.6 x 50mm Mobile Phase: HO (adjusted with Phosphoric Acid to a ph of.5): Acetonitrile (65:35) Flow Rate: ml/min Detector: UV at 4nm 3 4 Water Soluble Vitamins Min Ascorbic Acid. Nicotinic Acid 3. Pyridoxamine 4. Niacinamide 5. Folic Acid 6. Vitamin B 7. Riboflavin GraceSmart Columns Format i.d. (mm) Length (mm) Particle Size (µm) C8 Microbore Analytical Column: GraceSmart C8, 5μm, 4.6 x 50mm Mobile Phase: A: 0.M NH4HPO4 Buffer, ph 4.5 B: Acetonitrile Gradient: Time: %B: Flow Rate:.0mL/min Detector: UV at 54nm more info For detailed information on column formats, see page 3. technical assistance Contact Tech Support: [email protected] Online: 7

19 hplc columns & accessories hplc columns Vydac MS Columns Unsurpassed Resolution, Sensitivity and Recovery for Biomolecules Unique selectivity reveals peaks otherwise masked by other C8 columns Excellent peak shape with little or no TFA High protein recoveries make scale-up easy Vydac MS columns are the latest development in the ongoing effort to provide the best reversed-phase HPLC columns for biomolecules. A proprietary surface treatment and bonding process give Vydac MS columns truly unique selectivity. A variety of reversed-phases makes this product line suitable for small peptides to large intact, undenatured proteins. Vydac MS Phase Specifications Phase Pore Size (Å) Surface Area (m /g) Carbon Load (%) Endcapped Chromatographic Properties 8MS C Yes Polymeric bonding, highest hydrophobic interaction and unique geometric selectivity. 38MS C Yes Monomeric bonding offers increased peptide interaction and generally yields higher peak counts. 08MS C Yes Lower hydrophobicity is better for larger biomolecules. 4MS C Yes Lower capacity than C8 or C8, suitable for hydrophobic proteins or when minimal organic is desired. 9MS Diphenyl Application/ Benefit Use for simple enzymatic digests (< proteins) or biomolecules 0 5K MW. Use for same applications as 8MS, but offers different C8 selectivity. Ideal for biomolecules 5 0K MW. Ideal for biomolecules >0K MW, undenatured intact proteins, antibodies, oligonucleotides, human growth hormone Yes Lowest capacity, aromatic functional groups. Highly selective for proteins with aromatic side chains. USP L-code L L L7 L6 L Unique Selectivity Improved selectivity for peptides on the Vydac MS columns results in better primary structure definition and easier identification of degradation products and other protein characteristics. Tryptic Digest of Fetuin Vydac 38MS C8 Column Competitor C8 Column Competitor C8 Column Min. Columns: All 300Å, 5µm, 4.6 x 50mm Mobile Phase: A: 0.% v/v trifluoroacetic acid in HO B: 0.085% v/v trifluoroacetic acid in Acetonitrile Gradient: Time: %B: Flow Rate:.0mL/min. Detector: UV at 5nm Sample: 30µL of a 6µg/µL digest (80µg total peptide load) Sample components interfere with the separation of peptides on the Competitor and columns, appearing as a chromatographic hump. The unique selectivity of Vydac MS columns solves separation problems. Unsurpassed Recovery and Peak Symmetry Many proteins are bound to the cell membrane and are very hydrophobic. Although hydrophobic proteins are particularly difficult to separate, Vydac MS columns provide excellent recovery and peak shape for these molecules. Transmembrane Protein p Min kd Protein. Non-myristolyated p4 3. Myristolyated p4 4. Triton X (Surfactant) Vydac 4MS C4 Column Competitor C4 Column Competitor C3 Column Competitor 3 C4 Column Competitor 4 C4 Column Columns: All 300Å, 4.6 x 50mm packed with 5µm except 4µm for Competitor Mobile Phase: A: 0.% v/v trifluoroacetic acid in HO B: 0.085% v/v trifluoroacetic acid in Acetonitrile Gradient: Time: %B: Flow Rate:.0mL/min. Detector: UV at 5nm Reptilian reovirus RRV p4 sample courtesy of Drs. Roberto J. de Antueno and Roy Duncan, Dalhousie University, Halifax, Nova Scotia. The Vydac column provides better performance for this highly hydrophobic membrane protein (RRV p4) and its fatty acid modified (myristolyated) form. 8

20 hplc columns & accessories High Protein Recovery Unique surface chemistry reduces adsorption of proteins for higher recoveries and increases mass loading, which makes Vydac MS columns ideal for preparative chromatography. Excellent Peak Shape with Little or No TFA TFA is UV absorbent and contributes background at low UV wavelengths. It is additionally problematic with electrospray MS where it interferes with ion generation and reduces MS sensitivity. Percent Recovery Vydac MS Column Loading Amount: µg of Cytochrome C Competitor Competitor Vydac MS C4 column provides more than 0% better recovery for Cytochrome C. Vydac MS Columns Competitive Recovery Comparisons i.d. (mm) Length (mm) Particle Size (µm) 8MS (C8) 38MS (C8) TFA Concentration Comparisons on 8MS Columns 0.6 AU % TFA 0.0% TFA 0.0% TFA 08MS (C8) Min. 4MS (C4) 4 9MS (diphenyl). Neurotensin 8 (fragment). Oxytocin 3. Angiotensin II 4. Neurotensin Format Capillary MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS5.50 4MS MS MS MS5.55 4MS MS MS MS5.55 4MS MS MS MS MS MS MS MS5.30 4MS MS MS MS5.35 4MS MS MS MS5.35 4MS MS MS MS MS MS MS MS5.50 4MS MS MS MS5.55 4MS MS MS MS5.55 4MS5.55 Microbore MS505 38MS505 08MS505 4MS MS50 38MS50 08MS50 4MS50 9MS MS55 38MS55 08MS55 4MS MS5 38MS5 08MS5 4MS5 Holder Solvent MS53 4MS53 Reducer Analytical MS5405 4MS MS540 38MS540 4MS MS545 38MS545 08MS545 4MS545 9MS MS54 38MS54 08MS54 4MS54 Preparative MS MS50 4MS MS00 4MS MS05 4MS MS0 4MS0 Guard GD5MS 38GD5MS 08GD5MS 4GD5MS GD54MS 38GD54MS 08GD54MS 4GD54MS 800 Prep Guard GCC50 4GCC GCC GCC530 4GCC hplc columns 9

21 hplc columns & accessories hplc columns Vydac Everest Columns High Resolution for Complex Peptide Digests Exceptionally high peak counts for better characterization Unique selectivity for both hydrophilic and hydrophobic peptides Excellent sensitivity with little or no TFA in the mobile phase Everest columns have unique selectivity and sensitivity, which are the result of bonding technology that improves C8 surface coverage and deactivates residual silanols. Previously, the best 300Å C8 chemistries have had carbon coverage in the.8 to 3.6µmol/m range. Everest C8 coverage is in excess of 4µmol/m and approximates the theoretical limit based on surface area. This increased shielding of the base silica increases column life and reduces the amount of TFA required to shield the silica. Vydac Everest Phase Specifications Pore Size (Å) Surface Area (m /g) Carbon Load (%) Endcapped Chromatographic Properties Phase 38EV C Yes Maximum surface coverage for highest resolution of complex samples. Application/ Benefit Complex enzymatic digests (> proteins). USP L-code L Tryptic Digest of Bovine Serum Albumin Everest Column 0 peaks Commercial Column A 94 peaks Commercial Column B 96 peaks Min. Column: All C8, 300Å, 5µm, 4.6 x 50mm Mobile Phase: A: 0.% TFA in Water B: 0.085% TFA in Acetonitrile Gradient: Time: %B: Flow Rate:.0mL/min Detector: UV at 5nm Everest columns outperform competitor columns by providing higher-resolution separations (average of 7% higher peak counts than competitor columns tested). Vydac Everest Columns i.d. Length Particle Size 38EV (C8) Format (mm) (mm) (µm) Capillary EV EV EV EV EV EV EV EV EV EV EV5.55 Microbore EV EV EV EV EV EV5 Analytical EV EV EV54 Bovine Serum Albumin with no TFA Tryptic Digest Intensity (x 0 6 cps) Min. Column: Vydac Everest C8, 300Å, 5µm, 0.3 x 50mm Mobile Phase: A: 0.% v/v Formic Acid in Water B: 0.% v/v Formic Acid in Acetonitrile Gradient: Time: %B: Flow Rate: 5µL/min Detector: MS + ESI The Everest column performs exceptionally well with no TFA in the mobile phase, ensuring excellent microbore sensitivity. Vydac Everest Guard Columns i.d. (mm) Length (mm) Particle Size (µm) 38EV (C8) Holder Format Capillary EV5C030 GR-370A Guard Guard GD5EV GD54EV 800 more info For detailed information on column formats, see page 3. technical assistance Contact Tech Support: [email protected] Online: 0

22 hplc columns & accessories Vydac ProZap C8 Columns Ultra Fast Protein and Peptide Separations Ten times faster bioseparations than traditional columns Ultra-high efficiency.5μm packing For use with conventional HPLC systems The combination of ProZap.5µm, 500Å packings and short Expedite hardware delivers not only faster sample throughput, but also low back pressures suitable for conventional LC systems. Under gradient conditions, longer columns only increase run time and do not increase resolution. Proteins adsorb at the head of the column and then desorb with the critical mobile phase concentration. Since proteins do not interact with the full length of the packed bed, short columns are sufficient for full resolution. Separate proteins of broad molecular weight ranges in less than one minute. Vydac ProZap C8 Phase Specifications Phase Pore Size (Å) Surface Area (m /g) Carbon Load (%) Endcapped Chromatographic Properties Application/ Benefit USP L-code C Yes Subµm, 500Å wide pore. Ideal for fast, intact protein or peptide analysis. L hplc columns Seven Proteins in Under One Minute 6 7. Ribonuclease (37ng). Bovine Insulin (37ng) 3. Cytochrome C (37ng) 4. Lysozyme (4ng) 5. Bovine Serum Albumin (37ng) 6. Carbonic Anhydrase (37ng) 7. Ovalbumin (37ng) Synthetic Peptides 0 Injections Contaminant peptides/impurities. RGAGGLGLGK-amide (883 Da) 3. Ac-RGAGGLGLGK-amide (96 Da) 4. Ac-RGVGGLGLGK-amide (954 Da) 5. Ac-RGVVGLGLGK-amide (996 Da) Min. Column: ProZap C8,.5µm,. x 0mm, 500Å Mobile Phase: A: 0.% TFA in Water B: 0.085% TFA in Acetonitrile Gradient: Time: %B: Flow Rate: 0.8mL/min 6 Detector: 3UV at 80nm 7 5 Antibodies in Sheep Serum Min.. Albumin. IgG Min. Column: ProZap.0.5 C8, 3.0.5µm, x 0mm, 4.5 Min. 500Å Mobile Phase: A: 0.% TFA in Water B: 0.085% TFA in 90:0 n-propanol:water Gradient: Time: %B: Flow Rate: 0.5mL/min Detector: UV at 80nm Min. Column: ProZap C8,.5µm,. x 0mm, 500Å Mobile Phase: A: 0.% TFA in Water B: 0.085% TFA in 80:0 Acetonitrile:Water Gradient: Time: %B: Flow Rate: 0.8mL/min Detector: UV at 5nm Vydac ProZap Columns i.d. Length Particle Size Format (mm) (mm) (µm) Expedite more info For detailed information on column formats, see page 3.

23 hplc columns & accessories hplc columns Vydac TP Columns The Industry Standard for Polypeptide Separations Exceptionally long column lifetime and negligible phase leaching Reliable protein purifications, scalable from analytical to preparative scale The 300Å pores of TP silica provides polypeptides complete access to the interior of the silica. The unique process by which we manufacture Vydac TP silica results in high purity with carefully controlled characteristics. It is the standard that has defined large pore HPLC silica for polypeptide separations for nearly two decades. Vydac TP Phase Specifications Pore Surface Carbon Phase Size (Å) Area (m /g) Load (%) Endcapped Chromatographic Properties Application/ Benefit 8TP C Yes First generation polymeric C8 Small polypeptides 4 5K MW, enzymatic digest fragments, L media with unique selectivity. natural and synthetic peptides, multi-ring compounds. 38TP C Yes First generation monomeric C8 Use for same applications at 8TP, but offers different L media. C8 selectivity. 08TP C Yes Less hydrophobic than C8TP Polypeptides 0 0K MW. L7 media. 4TP C Yes First generation C4 media. Glycoproteins, hemoglobin variants, histones, insulin L6 variants, membrane proteins. L 9TP Diphenyl Yes Lowest capacity first generation diphenyl media. Polypeptides with aromatic side chains, large hydrophobic proteins, membrane-spanning peptides, lipid peptides, fusion proteins from inclusion bodies. USP L-code Standard Human Apolipoproteins apoc-iiia. apoc-iiib 3. apoc-iia 4. apoc-iiic 5. apoc-i 6. apoc-iib 7. apoa-ia 8. apoa-ib 9. apoa-iic 0. apoa-iia. apoa-iib The standard was human insulin. Insulin Variants Chicken. Bovine 3. Ovine 4. Rabbit 5. Human 6. Porcine 7. Rat I 8. Rat II Min. Column: Vydac 8TP C8, 5µm, 4.6 x 50mm Mobile Phase: A: 5% ACN/0.% TFA in Water B: 58% ACN/0.% TFA in Water Gradient: Time: 0 33 %B: 0 00 Flow Rate:.mL/min Column Temp: 50 C Min. Column: Mobile Phase: Vydac 4TP C4, 5µm, 4.6 x 50mm A: 0.% TFA B: ACN with 0.% TFA Gradient: Time: 0 5 %B: 7 30 From J. Rivier and R. McClintock, J. Chrom. 68, 9 (983). Comparison of Polypeptide Separations on C8, C4, and Diphenyl Reversed-Phase Columns Tryptic Digest 8TP (C8). H. TH6 4TP (C4) 9TP (diphenyl) Min. Column: Vydac 08TP C8, 5µm, 4.6 x 50mm Mobile Phase: A: 0.% TFA B: ACN with 0.% TFA Gradient: Time: 0 60 %B: 0 30 Flow Rate:.0mL/min Min. Columns: Vydac 8TP C8, 4TP C4 and 9TP diphenyl, all 5µm, 4.6 x 50mm Gradient: Time: 0 30 %B: 0 90 Sample: 8-residue helical peptide () and six-helix template assembled synthetic protein ()

24 AU hplc columns & accessories Monomeric and Polymeric Bonded Phases Comparison 3,4 8TP54 Polymeric C8 8, TP54 Monomeric C8 4, Min.. GY. VYV 3. Neurotensin fragment 8 (pelyenkpr) 4. Ac-RGGGGLGLGK-NH 5. RGAGGLGLGK-NH 6. Ac-RGAGGLGLGK-NH 7. Ac-RGVGGLGLGK-NH 8. Oxytocin (CYIQNCPLG-NH) 9. Met Enkephalin (YGGFM) 0. Bradykinin (RPPGFSPFR). Ac-RGVVGLGLGK-NH. Neurotensin Fragment 8 3 (RRPYIL) 3. Angiotensin II (DRVYIHPF) 4. Leu Enkephalin (YGGFL) 5. Neurotensin (pelyenkprrpyil) 6. Angiotensin I (DRVYIHPFHL) Columns: Vydac 8TP and 38TP, both C8, 5µm, 4.6 x 50mm Gradient: Time: 0 30 %B: 0 40 Flow Rate:.0mL/min Detector: UV at 0nm 0.0 AU Purification of a Lipid Peptide 0.4mg Preparative Chromatogram 60 Min Min. Column: Vydac 9TP Diphenyl, 5µm, 4.6 x 50mm Mobile Phase: A: 9% n-propanol/0.5% HOAc/70.5% water B: 6% n-propanol/0.5% HOAc/38.5% water Gradient: Time: %B: Flow Rate: 0.75mL/min Detector: UV at 80nm Sample courtesy of Dr. Don Diamond (City of Hope National Medical Center, Duarte, Calif.). For a complete description of this method development, request Vydac Application Note #980. hplc columns A synthetic peptide containing 5 amino acid residues and two attached fatty acids was difficult to purify because of its limited solubility and tendency to aggregate. The lower retention of the Vydac 9TP54 diphenyl reversed-phase column was useful for purification of this peptide after it was found to be retained so strongly that it could not be eluted from a C4 reversed-phase. Vydac TP Columns i.d. (mm) Length (mm) Particle Size (µm) 8TP (C8) 38TP (C8) 08TP (C8) 4TP (C4) 9TP (Diphenyl) Format Nano TP505 38TP505 08TP505 4TP505 9TP TP50 38TP50 08TP50 4TP TP55 38TP55 08TP55 4TP55 9TP TP5 38TP5 08TP5 4TP5 9TP5 Microbore TP505 38TP505 08TP505 4TP505 9TP TP50 08TP50 4TP50 9TP TP55 38TP55 08TP55 4TP55 9TP TP5 38TP5 08TP5 4TP5 9TP5 Solvent Reducer TP535 38TP535 4TP535 9TP TP53 38TP53 08TP53 4TP53 9TP53 Analytical TP TP3405 4TP TP340 38TP340 08TP340 4TP TP TP TP5405 4TP5405 9TP TP540 38TP540 08TP540 4TP540 9TP TP545 38TP545 08TP545 4TP545 9TP TP54 38TP54 08TP54 4TP54 9TP54 Preparative TP505 4TP TP50 38TP50 08TP50 4TP50 9TP TP505 4TP TP005 4TP TP00 4TP TP05 4TP TP0 08TP0 4TP0 9TP TP05 Guard* GD5 38GD5 08GD5 4GD5 9GD GD54 38GD54 08GD54 4GD54 9GD54 *Requires All-Guard Holder, 800. more info For detailed information on column formats, see page 3. technical assistance Contact Tech Support: [email protected] Online: 3

25 hplc columns & accessories hplc columns Alltech IC Columns Separate Organic and Inorganic Anions and Cations Meets various EPA method requirements Columns available for both suppressed and non-suppressed conductivity Polymer-based columns have broad ph applicability Alltech Ion Chromatography Phase Specifications Phase Base Material Functional Group Anion Columns Allsep Anion Methacrylate Quaternary ammonium Novosep A- Polyvinyl alcohol Quaternary ammonium AN Polystyrenedivinylbenzene Dimethyl ethanol ammonium Anion/S Silica Quaternary ammonium Anion/R Polystyrenedivinylbenzene Trimethyl ammonium Cation Columns Universal Cation Universal Cation HR Specialty Columns Anion Exclusion Silica, 7µm Silica, 3µm Polystyrenedivinylbenzene Polybutadiene/ maleic acid copolymer Polybutadiene/ maleic acid copolymer ph Range Organic Modifier Limitations Application/Benefit % Meets requirements for EPA method part A. Use for inorganic anions, weak and strong acid ions, metal complexes and organic acids 3 0 0% Ideal for separation of seven common anions plus three oxyhalide anions in one run. Meets multiple EPA method requirements. 3 0 Use for inorganic anions, weak and strong acid ions, and organic acids % Use for inorganic anions with the exception of fluoride. Silica-based for symmetrical peak shapes. 0 00% Larger dimensions ideal for high-resolution separations. Use for inorganic anions, weak and strong acid ions % Separates Group I and II cations in one isocratic run. Separates transition metals without postcolumn reaction. EPA Method part A Conductivity Mode suppressed and non-suppressed 300.0, suppressed 300., 37.0, 36.0 suppressed and non-suppressed non-suppressed non-suppressed suppressed and non-suppressed % For high resolution applications suppressed and non-suppressed Sulfonate <0% acetonitrile, no methanol, <5% ethanol Use for organic acids and weak acid anions. Add small amounts of acetonitrile to decrease retention of hydrophobic molecules. Surfactant C8 Silica, 5µm C % Use for long chain or aromatic surfactants such as xylene sulfonate and dodecyl benzene sulfonate. non-suppressed non-suppressed Resolves Fluoride Away from the Water Dip 3033 Anions in High Ionic Strength Water by EPA Method Fluoride. Chloride 3. Nitrite 4. Bromide 5. Nitrate 6. Phosphate 7. Sulfate Fluoride. Chlorite 3. Bromate 4. Chloride 5. Nitrite-N 6. Bromide 7. Chlorate 8. Dichloroacetate 9. Nitrate-N 0. Phosphate-P. Sulfate Min Min. Column: Mobile Phase: Flow Rate: Detector: Allsep Anion, 7µm, 4.6 x 00mm 0.85mM NaHCO3/0.9mM NaCO3.mL/min Suppressed Conductivity Column: Mobile Phase: Flow Rate: Detector: Novosep A- Anion, 5µm, 4.0 x 50mm 3.6mM NaHCO3 0.8mL/min Suppressed Conductivity 4

26 hplc columns & accessories Separation of Sulfite and Sulfate Fluoride. Chloride 3. Nitrite 4. Bromide 5. Nitrate 6. Phosphate 7. Sulfite 8. Sulfate 3 4 EDTA and Other Anions 884. Nitrite. EDTA 3. Bromide 4. Nitrate hplc columns Min Min. Column: Mobile Phase: Flow Rate: Detector: AN, 9µm, 4.6 x 50mm.8mM NaHCO3,.8mM NaCO3.0mL/min Suppressed Conductivity Column: Mobile Phase: Flow Rate: Detector: Anion/S, 0µm, 4.6 x 00mm.5mM Sulfuric Acid.0mL/min UV at 0nm Weak Acid Anions 5697 Metal Ions Borate (5ppm). Silicate (0ppm) 3. Formate (0ppm) 4. Sulfide (0ppm) 5. Chloride (0ppm) 6. Cyanide (0ppm) 3 4. Lithium (0.ppm). Sodium (.5ppm) 3. Ammonium (.5ppm) 4. Potassium (.5ppm) Nickel (.5ppm) 6. Zinc (.5ppm) 7. Cobalt (.5ppm) 8. Magnesium (.0ppm) 9. Calcium (.0ppm) Min Min. Column: Mobile Phase: Flow Rate: Detector: Anion/R, 0µm, 4. x 50mm 4mM Sodium Hydroxide/ 0.mM Sodium Benzoate.5mL/min Conductivity Column: Mobile Phase: Flow Rate: Detector: Universal Cation, 7µm, 4.6 x 00mm mm Tartaric Acid/mM Oxalic Acid.0mL/min Conductivity Alltech Ion Chromatography Columns i.d. (mm) Length (mm) Allsep Anion Novosep A- AN Anion/S Anion/R Universal Cation Universal Cation HR Anion Exclusion Surfactant C8 Format Analytical ** ** 6909** Rocket Metal Free Guard* *Requires All-Guard Holder, 800. **These columns are 4.mm i.d. These columns are 4.0mm i.d. more info For detailed information on column formats, see page

27 hplc columns & accessories hplc accessories High-Pressure Fittings One-Piece High-Pressure Fittings Soft grip elastomer cushion makes hand-tightening comfortable PEEK or Graph-Tite material is inert and biocompatible One-piece integrated ferrule eliminates handling small pieces Fittings with No-Slip feature stay on tubing between connections One-Piece Fitting Specifications Material: PEEK or Graph-Tite (Carbon-Reinforced PEEK) Max. Temperature: 00 C Max. Pressure: 5000psig Thread Type: 0-3 UNF Typical Use: /6" o.d. Tubing Connections One-Piece High-Pressure Fittings, 0/pk Standard No-Slip Graphic Length Color Material Soft Grip One-Piece Fittings A Short Black Graph-Tite B Long Black Graph-Tite Finger-Tight One-Piece Fittings C Short Natural PEEK D Long Natural PEEK 335 Hex-Head One-Piece Fittings E Short Natural PEEK 336 E Short Black Graph-Tite 3347 F Long Natural PEEK 338 A B C D E F Valco High-Pressure Fittings Valco Fittings Specifications Material: 36 Stainless Steel, 303 Stainless Steel, PEEK Max. Temperature: 500ºC (SS) 00ºC (PEEK) Max. Pressure: 0,000psig (SS) 8000psig (PEEK) Typical Use: High-Pressure Tubing Connections Valco Nuts, Ferrules and Unions Graphic Qty. A /6" Stainless Steel Nut B /6" (Type 36) Stainless Steel Ferrule C /6" PEEK Ferrule D /6" Internal Union 0.5mm Bore ea 3073 D /6" Internal Union 0.50mm Bore ea 3074 D /6" Internal Union /6" Bore ea A B C D Two Piece High-Pressure Fittings Two-Piece Fitting Specifications Material: Wing-Nut: PEEK Hex-Head: 300 Stainless Steel Max. Temperature: Wing-Nut: 00 C Hex-Head: 500 C Max. Pressure: Wing-Nut: 6000psig Hex-Head: 0,000psig Thread Type: 0-3 UNF Typical Use: /6" o.d. Tubing Connections Two-Piece Fittings Graphic Qty. Two-Piece PEEK Wing-Nut Fitting with Ferrule A Natural Two-Piece PEEK Wing-Nut Fittings Replacement Ferrules B Ferrules Two-Piece Stainless Steel Hex-Head Fittings C /6" Nut, Short D /6" Nut, Long Two-Piece Stainless Steel Hex-Head Replacement Ferrule E /6" Ferrules A B C D E 6

28 hplc columns & accessories Low-Pressure Fittings Flange-Free Low-Pressure Fittings Convenient ferrule eliminates need for flanging Compatible with all /4-8 UNF ports Flanged Tubing Low-Pressure Fittings Use with /8" or /6" flanged tubing Easily tighten without a tool Flanged-Free Tubing Fitting Specifications Material: PEEK, Kel-F, ETFE Maximum Temperature: 00 C Maximum Pressure: /8" 500psig, /6" 000psig Thread Type: /4-8 UNF Typical Use: /6" or /8" o.d. Tubing Connection Flanged Tubing Fitting Specifications Material: PEEK with Stainless Steel Washers Maximum Temperature: 00 C Maximum Pressure: Limited by Tubing Strength Thread Type: /4-8 UNF Typical Use: Low-Pressure Plumbing Low-Pressure Fittings, 0/pk /6" o.d. Tubing /8" o.d. Tubing Graphic Material Flange-Free Finger-Tight Nuts A Natural PEEK Flange-Free Ferrules B Ferrules Kel-F B Ferrules ETFE Flanged Tubing Fittings (washer included) C Natural PEEK A B C hplc accessories Prefilters Direct-Connect Filter Kit Threads into column inlet for minimal dead volume Interchangeable tips connect to all column formats Inexpensive replaceable filter elements Soft Grip Fitting Stainless Steel Filter Housing Tips In-Line Filter Kit Available in metal-free or 36 stainless steel Comes with connecting fittings Replaceable filter element Metal-Free In-Line Filter Stainless Steel In-Line Filter Universal Mini Filters Low volume direct-connect design Ideal for LC/MS applications Depth filter for greater capacity without band spreading Patent #5, 55, 503; #5,730,943; #5,9,954. Rheodyne Column In-Line Filter Kit Use 3mm filter kit for 4.6mm columns Use.5mm filter kit for and mm columns Includes one holder, one filter, three fittings and two connecting tubes 6cm x 0.007" i.d. Prefilters Material Max. Temperature. Max. Pressure Thread Type Porosity Qty. Direct-Connect Filter Kit and Replacement Parts Direct Connect Filter Kit 36 SS, PTFE, and Kel-F 00 C 5000psig 0-3 UNF.0µm ea 8689 Housing 36 Stainless Steel 00 C 5000psig 0-3 UNF ea 7005 Filter Stainless Steel 00 C 5000psig 0-3 UNF.0µm 5/pk 8640 In-Line Filter Kit Metal-Free Filter Kit* PEEK, PAT, and Graph-Tite 00 C 5000psig 0-3 UNF.0µm ea 6850 Stainless Steel Filter Kit** 36 SS and Graph-Tite 00 C 5000psig 0-3 UNF.0µm ea 7000 Universal Mini Filters Mini Filter.µL Internal Volume 36 Stainless Steel 00 C 7000psig 0-3 UNF 0.5µm 5/pk 7766 Mini Filter.µL Internal Volume 36 Stainless Steel 00 C 7000psig 0-3 UNF.0µm 5/pk 7767 Mini Filter 0.3µL Internal Volume 36 Stainless Steel 00 C 7000psig 0-3 UNF.0µm 5/pk 7768 Rheodyne Column In-Line Filter Kits and Replacement Parts 3mm Filter Diameter Kit 36 Stainless Steel 500 C 7000psig 0-3 UNF 0.5µm ea 7335RV 3mm Filter Element 36 Stainless Steel 500 C 7000psig 0-3 UNF 0.5µm ea *Includes PEEK holder, PAT filter, and two soft grip fittings. **Includes 36 stainless steel holder, stainless steel filter, and two soft grip fittings. 7

29 hplc columns & accessories hplc accessories Adapters High-Pressure Adapters, Plugs, and Caps High-Pressure Adapters, Plugs and Caps Specifications Material: 300 Stainless Steel, PEEK Max. Temperature: 300 Stainless Steel: 500 C PEEK: 00 C Max. Pressure: 300 Stainless Steel: 0,000psig with Stainless Steel Ferrules PEEK: 8000psig Thread Type: 0-3 UNF Typical Use: /6" o.d. Tubing Adapters, Plugs, and Caps Graphic Bore Qty. Stainless Steel Adapters A Union, Female 0.0" ea 867 A Union, Waters 0.0" ea 9733 B Bulkhead Union ea 3505 C Tee ea 869 PEEK Adapters D Union 0.00" ea 34 E Tee 0.00" ea 343 Stainless Steel Plugs F Standard (7000psig) ea 3063 Stainless Steel Cap G Standard ea Low-Pressure Adapters and Plugs Chemically inert /4-8 threaded ports For use with Flange-Free or flanged fittings /4-8 Low-Pressure Adapters and Plugs Graphic Material Qty. Unions A Union PEEK ea 0088 B Bulkhead Union PEEK ea 3506 Tees C 0.8mm Bore Tee PEEK ea 0058 C.5mm Bore Tee PEEK ea 003 Crosses D 0.8mm Bore Cross PEEK ea 0059 D.5mm Bore Cross PEEK ea 005 Male /4-8 Adapters E Male /4-8 to Male Luer ETFE ea 009 F Male /4-8 to Female Luer ETFE ea 0055 Plugs G Natural PEEK H White Kel-F A B A B C C D D F E F G H G One-Piece Couplers One-Piece PEEK couplers have a non-wetted color-coded POM handle. Stop depth of the tip is fixed and provides a reliable connection when used with matching port configurations. E best value Easy-Flange Tool Uses mechanical force to form flanges no electricity or heat required Forms perfect flanges on /6" to /8" o.d. PTFE or thin-walled PEEK tubing Completely portable use it anywhere in your lab One-Piece Column Couplers Port Configuration Color Female Black 89 Waters Red 89 Male Red 893 Valco Blue 894 Easy-Flange Tool Easy-Flange Flanging Tool* Replacement Parts Clean-Cut Tubing Cutter 3590 *Includes: Clean-Cut tubing cutter and insert disks 35908, 35907, 35906, 3590,

30 hplc columns & accessories Valves and Sample Loops Rheodyne Model 775i Analytical Injectors "Make-Before-Break" (MBB) switching for uninterrupted flow Wider port angles provide improved fitting access Position sensing switch option Capable of injecting volumes from µl to 5mL with high precision Rheodyne Model 700 Analytical Injectors Rear-loading valves High volumetric precision Low volume flow passages Loop-Filler port accommodates needles from 0.08" to 0.08" diameter Loop Filler Port Accessory Analytical Sample Injectors Version 775i, Includes a 0µL PEEK Sample Loop SS , Includes a 0µL Sample Loop SS 700RV Rheodyne Sample Loops Metal Sample Loops Square cut and burr free tubing for flush connection to the valve Includes unswaged fittings and ferrules Coiled and configured for specific valves Metal-Free Sample Loops PEEK construction is inert and biocompatible Flexible tubing makes them easy to use Includes versatile RheFlex fittings hplc accessories Rheodyne Manual Switching Valves Rheodyne HPLC switching valves offer an endless variety of ways to simplify switching and injecting. Standard valves are supplied with 0.04" internal flow passages and are suitable for use with analytical and semi-preparative applications. Rheodyne Manual Switching Valves Version Bore -Position Valves 6-Port SS Standard 7000RV 3-Way SS Standard 7030RV Multi-Position Valves 6-Position 6-Port SS Standard 7060RV Sample Loops Analytical Injectors Metal 775/775i Metal 75/700 Metal 85/86 PEEK 9000/975 5µL SL µL SL µL SL µL SL µL* SL µL* SL µL* SL ml* SL ml* SL mL* SL *Also compatible with 85/86. Rheodyne Replacement Parts Replacement Parts for Rheodyne Injectors Rotor Seal for Stator for 700/ Rotor Seal for 75/76/775/775i ETFE Rotor Seal for Stator for 775/775i Stator Face Assembly for 775/775i Rotor Seal (Polyimide) for ETFE Rotor Seal for technical assistance Contact Tech Support: [email protected] Online: Rheodyne RheBuild Kits for Injection Valves Rheodyne RheBuild Kit Specifications Model Includes 75, 76, 775, 775i, 776 Standard Rotor Seal, Stator Face Assembly, Isolation Seal, Needle Guide, Needle Port Cleaner, Hex Keys and Instructions 700, 7000 Standard Rotor Seal, Isolation Seal, Hex Keys and Instructions Rheodyne RheBuild Kits 75, , 775i, ,

31 hplc columns & accessories hplc accessories Mobile Phase Management Solvent Filtration Apparatus The solvent filtration apparatus rapidly filters and degasses HPLC and IC mobile phases. Solvent Filtration Apparatus Solvent Filtration Apparatus L Flask, 300mL Funnel, 47mm Filter Support, 00 47mm Clamp, 0.µm Nylon Filters 00/pk L Flask, 500mL Funnel, 47mm Filter Support, mm Clamp, 0.µm Nylon Filters 00/pk Replacement Parts and Accessories L Flask (For 003) mL Glass Funnel (For 00) mL Glass Funnel (For 003) mm Fritted Base Support (For 00) mm Fritted Base Support (For 003) mm Nylon Filter Membranes, 0.µm, 00/pk 034 Mobile Phase Caps Most economical way to helium sparge and deliver HPLC mobile phases MP-Cap has three /4-8 threaded ports with throughholes*; tubing may be pushed through or connected with flanged fittings MP-Cap has three unthreaded holes* for tubing insertion *(x/8" and x/6") MP-Cap MP-Cap Mobile Phase Caps* Qty. Thread Size MP-Cap ea GL MP-Cap ea GL *Not intended for build-up of helium environment. Vacuum Filtration System Eliminate solvent transfer steps with direct filtration Interchange funnels and in-line filter cap handle any filtration volume Durable polypropylene construction 36 Stainless Steel Slip-On Inlet Filter Economical mobile phase inlet filtration Maximum temperature 500 C 36 Stainless Steel Slip-On Inlet Filters Porosity Qty. For 0.06" (.5mm) i.d. µm For 0.06" (.5mm) i.d. 0µm Base Base Metal-Free Inlet Filter Ideal for IC or biochromatography applications PEEK body with replaceable polyethylene filter elements Maximum temperature 00 C Filter Funnel System In-Line Filtration System Vacuum Filtration Systems L Filter Funnel System 6409 Includes: Base, Adapter Kit, Mesh Filter Support, L Funnel L Filter Funnel System 649 Includes: Base, Adapter Kit, Mesh Filter Support, L Funnel In-Line Filtration System 6407 Includes: Base, Adapter Kit, Mesh Filter Support, In-Line Filter Cap Replacement Mesh Filter Support, 47mm Stainless Steel 64 Metal-Free Inlet Filters Porosity Qty. For /8" o.d. 0µm ea 370 For /6" o.d. 0µm ea 37 Replacement Filter Elements 0µm

32 flash & tlc Reveleris X Flash Chromatography System Grace has revolutionized flash chromatography by combining decades of experience in silica gel and evaporative light scattering detection technologies to create the Reveleris X flash chromatography system. Patented RevealX detection technology accelerates purification by simultaneous peak collection, triggered by UV and Evaporative Light Scattering Detection (ELSD). CONFIDENCE Maximize Purity and Recovery of your Target with RevealX Detection Technology Synchronized detection and fraction collection from up to 4 detector signals (ELSD/UV/Vis) Detects the widest range of compounds Maximizes recoveries with only 50ul/min maximum sent to ELSD FLEXIBILITY Purify a Broad Range of Sample Types with Selectable High/Low Detection Sensitivity Detect chromophores, nonchromophores, and visible sample components from mg to kg quantities Purify complex samples from discovery through process R&D Easily switch between normal-phase and reversed-phase applications flash U. S. Patents Nos. 8,5,930 8,305,58 8,305,58 8,34,934 SIMPLICITY One-Screen Programming and Run Control with Intuitive Windows Interface, Accelerates System Set-up Quickly modify run parameters on a large single screen display Edit gradients in real time, using click and drag or table Auto-recognition of cartridges and trays reduces setup time and the risk of potential errors Visit Our Website to Learn More: Detailed specifications and system information Service and support program information Application notes and technical articles Product information Reveleris X Flash Chromatography System Reveleris X Flash Chromatography System Reveleris X Flash Chromatography System UV/Vis Optional Upgrade Reveleris X Navigator Feature Optional Upgrade technical assistance Contact Tech Support: [email protected] Online: 3

33 flash & tlc flash Flash Chromatography Flash chromatography is a simple, often automated, low to medium pressure liquid purification technique used to rapidly isolate desired compounds or reaction intermediates from simple or complex mixtures. Purifying these important components at sufficient quantity and purity can be challenging when performing a flash separation using bare silica. Selecting an appropriately bonded stationary phase with suitable alternate mobile phases may be necessary since: Mixtures vary in complexity, including the number of components, compound class, polarity, and molecular weight, etc. Bare silica may not be the optimal stationary phase for the compounds due to lack of retention, solubility, poor peak shape and compound stability, etc. Selecting the Optimum Stationary Phase Media phases differ in polarity according to the graphic below and changing stationary phases is a powerful tool to achieving successful flash separations. Polar Silica Si-OH Amino Diol C8 Si-CH NH Si-H(OH)CH (OH) SI-(CH ) 7 CH 3 Grace Flash Cartridges Non-Polar Media Selection Guide Phase Selection Guide Compound Class Si C8 Amino Diol Alkaloids X X X Amino Acids X Aromatics X X X X Carbohydrate X X Drug Intermediates X X Basic Drug Products X X X Flavonoids X Glycosides X X X Isomers X X Lipids X X Lignans X X Polyphenols X X Saponins X X Steroids X Tannins X Terpenes X X X Vitamins X X X X Xanthones X X X Peptides <5000mw X X Oligonucleotides <5000mw X Nucleosides/tides X Pegylated X X Secondary Metabolites X Tips For compound classes for which multiple media options are indicated, phase preference is further determined by the extent of sample component acidic/basic nature and overall polarity. For maximum resolution, reversed-phase C8 using aqueous mobile phase is often selected as the primary option, especially for polar samples having a strong affinity for silica. For difficult purifications of closely-related compounds, smaller particle media packings or higher pressure techniques may need to be employed to achieve separation. Larger, sensitive or charged molecules may require lower flow and pressures common for ion exchange, size exclusion or affinity chromatographic techniques (vaccines, DNA, intact proteins, and peptides >5KDa). Get the most out of your flash applications by choosing Grace flash cartridges, the only flash cartridges designed by a silica expert for ideal chromatographic performance. Grace has balanced small particle size media with high loading capacity and efficient packing methods to produce the perfect flash cartridge. Why Do Grace Cartridges Perform Better?. Small Particle Size A smaller particle size increases resolution. Tighter particle size distribution and minimal fines keep back pressure low.. High Purity Silica Grace's 30 years of chromatography experience combined with 80 years of silica manufacturing expertise has allowed Grace to produce optimized silica designed for flash chromatography. 3. Efficient Column Packing A tightly packed silica bed increases cartridge efficiency and prevents voids and channeling that cause poor resolution. Flash chromatography, like HPLC, benefits greatly from optimized packing procedures. 4. Strict Quality Control Grace flash cartridges are characterized with more than 5 tests from the silica to the packed cartridges to ensure consistent lot-to-lot performance. A quality assurance certificate is provided with each box of cartridges. 3

34 flash & tlc Reveleris Flash Cartridges Superior resolution Increased loading capacity High pressure (00 psig/4bar)* Auto-recognition by Reveleris flash system The silica packed in Reveleris cartridges is specifically designed to increase cartridge efficiency and therefore resolution of closely eluting compounds, in some cases greater than two times better resolving power over typical packings. Greater efficiency enables more peaks to be baseline resolved and results in increased sample purity as well as decreased fraction volumes. Additionally, by increasing sample loads on a given cartridge size and elevated pressure capability, sample throughputs and yields increase and overall purification costs are lowered. Luer design is compatible with most systems. Reveleris flash cartridge Loading Capacity Comparison Competitor flash cartridge. Toluene. Dimethyl Phthalate Amount of Sample Loaded 0.48g 3.84g 6.7g 9.g 9.6g flash Min Min. Cartridge: 40g silica cartridge Mobile Phase: Hexane/Ethyl Acetate (70/30) Flow Rate: 40mL/min Detection: UV at 54nm The Reveleris flash cartridge maintains baseline resolution up to 9.g, while a leading competitor cartridge only maintains resolution to 3.8g of load. Unique Chemistries for Challenging Applications While silica is the most popular choice for flash applications, other chemistries bring unique properties that can help resolve complex samples. Chemistry Ideal For Silica Conventional applications, polar compounds Silica HP High resolution for complex or difficult to purify samples Reversed-Phase (C8) Non-polar compounds C8-WP High resolution wide pore media for large molecule purification; ideal for peptides, proteins, lipids Amino Better peak shapes with polar and basic compounds; ideal for carbohydrates Diol Alternate selectivity for a variety of polarity types; ideal for lipids more info Visit to browse the latest applications using Reveleris flash cartridges. Reveleris Cartridges (up to 00psig*) Cartridge Size Reveleris Bonded Flash Cartridges (up to 00psig*) Cartridge Size Amino + Diol Silica C8 Silica HP Qty. Silica 4g Silica g Silica 4g Silica 40g Silica 80g Silica 0g Silica 0g* Silica 330g* C8-WP Qty. 4g ea g ea g ea g ea g ea g ea Also available in packages of 0. *0g and 330g have a pressure rating of 00 psig. 33

35 flash & tlc GraceResolv Flash Cartridges Choice of luer slip or lock outlet connection Universal luer fitting Higher resolution Increased loading capacity Reproducible performance Silica cartridge sizes 4g 500g flash GraceResolv cartridges are packed with a grade of Davisil silica designed to help maximize performance of today s flash instruments. GraceResolv LOK cartridges include a luer lock outlet and offer the same great perfomance benefits of GraceResolv cartridges. GraceResolv LOK cartridges are offered for use with instruments requiring both luer lock inlet and outlet connections. The cartridges are supplied with an adapter that allows connection to systems using a luer slip outlet for maximum flexibility. Luer design is compatible with most systems. Increase Resolution and Product Purity with GraceResolv Flash Cartridges Leading Competitor Cartridge Compound Not Resolved 3 4 Min.. Acetylacetone H 3 C CH 3 O O Methyl Paraben Pure Fraction 0.70 O Completely HO Resolved 0.60 OCH Cartridge Size: g Sample: Acetylacetone, Methyl Paraben dissolved in 95:5 Hexane:IPA with % TFA Mobile Phase: 80:0 Hexane:Ethyl Acetate Flow Rate: 36mL/min Detection: UV 54nm GraceResolv Flash Cartridge 3 4 Min. GraceResolv Flash Cartridges GraceResolv GraceResolv LOK Cartridge Size Qty. Silica 4g Silica g Silica 4g Silica 40g Silica 80g Silica 0g Silica 0g Silica 330g Silica 750g Silica 500g more info For additional information about flash chromatography, including application notes and technical posters, visit our website. technical assistance Contact Tech Support: [email protected] Online: 34

36 flash & tlc Flash Accessories and TLC Solid Loaders High pressure design Easy to use Ideal for low solubility loading Reveleris Flash Chromatography System Verification Kits Kits include 0 tubes with preabsorbed text mix and five g flash cartridges Solid Loaders Qty. Empty 3g Empty 5g Empty 40g Silica 3g Silica 5g Silica 40g Adjustable 3g Plunger ea Adjustable 5g Plunger ea 5485 Adjustable 40g Plunger ea g Frit Insertion Rod ea g Frit Insertion Rod ea g Frit Insertion Rod ea 5690 Reveleris Method Development TLC Plates and C8 Scouting Columns One of the first steps in scale-up of flash chromatography separations is to optimize the mobile phase. Two methods are commonly used to determine the optimum mobile phase composition. Resulting data is used to automatically generate flash gradient profiles in the Reveleris Navigator software. For normal phase purifications, thin layer chromatography (TLC) plates made with the same high purity silica as used in the Reveleris and GraceResolv cartridges are used to ensure the same selectivity and allow simple scale-up. For reversed-phase applications, Reveleris C8 scouting columns are the perfect method development tool to optimize mobile phase choice. Flash TLC Plates Size Qty. GraceResolv Silica TLC Plates 0 x 0cm Reveleris Silica TLC Plates 0 x 0cm Reversed-Phase C8 TLC Plate 0 x 0cm Silica Aluminum-backed TLC Plates (UV54) 0 x 0cm Silica Aluminum-backed TLC Plates (no UV) 0 x 0cm Reveleris System Verification Kits Reveleris System Verification Kit High Concentration Flash Test Mix Reveleris ies System Verification Kit Low Concentration Flash Test Mix Flash Cartridge Kits Optimized for specific application areas Method development packs Flash Cartridge Kits Reveleris Silica Cartridge Select Pack () 4g Si, () g Si, () 40g Si Radiochem Flash Kit (3) 4g Si, (3) 4g C8, (3) 4g Diol, (0) 3g Loaders Impurity Isolation Flash Kit () g C8, () g Diol, () 0g C8, () 0g Diol Natural Product Flash Kit (3) g C8, (3) g Si, (3) 3g Loaders Carbohydrate Flash Kit (3) g C8, (3) g Amino Lipid Flash Kit (3) g Si, (3) g C8, (3) g Diol Flash System Adapters Flash System Adapters Reveleris Flow Diverting Bypass Kit Bypasses the Column Position to Connect Large Cartridges to the Reveleris Flash System Includes Bypass Tube ( 86700), Fittings, and Tubing GraceResolv 750g Flash Cartridge Adapter Kit Adapts GraceResolv 750g Cartridge to the Reveleris Flash System Also Requires Flow Diverting Bypass Kit ( ) GraceResolv 500g Flash Cartridge Adapter Kit Adapts GraceResolv 500g Cartridge to the Reveleris Flash System Also Requires Flow Diverting Bypass Kit ( ) flash Reveleris C8 HPLC Columns Reveleris C8 HPLC Columns* Size Qty. C8 HPLC Column 4.6 x 50mm C8 HPLC Column 4.6 x 50mm C8 HPLC Column 4.6 x 50mm *For use with Reveleris Navigator Software. 35

37 flash & tlc tlc TLC Plates Adsorbosil Soft Layer TLC Plates Contains no organic binders Sample zones are easy to remove for further analysis Soft Layer Adsorbosil Prekotes TLC Plates Layer Size Thickness Qty. Soft Layer Prekotes, Glass-Backed Conventional Adsorbosil -Plus 0 x 0cm 50µm Adsorbosil -Plus P* 0 x 0cm 50µm Adsorbosil -Plus 0 x 0cm 50µm Adsorbosil -Plus P* 0 x 0cm 50µm 5 63 *P = With Fluorescent Indicator, 54 wavelength. Macherey-Nagel TLC Plates 60Å pore size, 5 to 7µm particle size Binder is organic, stable in most organic solvents and aggressive detection reagents Macherey-Nagel TLC Plates Size Layer Thickness Qty. Silica Gel Plates, Glass-Backed Conventional Layers, Silica Gel 60 SIL G-5 5 x 0cm 50µm SIL G-5 5 x 0cm 50µm SIL G-5 0 x 0cm 50µm SIL G-5 UV54 5 x 0cm 50µm SIL G-5 UV54 0 x 0cm 50µm SIL G-5 UV x 0cm 50µm Polyester-Backed TLC Plates Silica Gel 60 SIL G 0 x 0cm 50µm SIL G/UV54 4 x 8cm 50µm SIL G/UV 54 0 x 0cm 50µm SIL N-HR/UV54 0 x 0cm 00µm Analtech Uniplates TLC Plates Silica Gel H and HF Uniplates TLC Plates Soft Layer Contains no organic binder Contains no calcuim sulfate binder Hard Layer Contains an organic binder 80% water resistant Visualization by charring up to 50ºC Silica Gel H and HF Uniplates TLC Plates Layer H HF* Thickness Qty. Soft Layer, No Binders Conventional 0 x 0cm 50µm x 0cm 50µm Hard Layer, Organic Binder Conventional 0 x 0cm 50µm x 0cm 50µm *F-Fluorescent Indicator, 54nm. Silica Gel G and GF Uniplates TLC Plates Calcium Sulfate Binder Contains no organic binder Silica Gel G and GF Uniplates TLC Plates Layer G GF* Thickness Qty. Soft Layer, No Binders Conventional 0 x 0cm 50µm x 0cm 50µm *F-Fluorescent Indicator, 54nm. more info For additional information about flash chromatography, including application notes and technical posters, visit our website. technical assistance Contact Tech Support: [email protected] Online: 36

38 flash & tlc TLC Accessories TLC Tanks and Storage Latch-Lid TLC Tank Unique latching device assures tight seal between lid and tank No messy "grease" seals Latch-Lid Tank Size Latch-Lid Tank/Lid Unit 0 x 0cm Plates 754 Latch-Lid Tank/Lid Unit 0 x 0cm Plates 7536 Multiple Plate Development Rack Saves time and money TLC Plate Cutter Scores coated glass plates at any position Compact, portable, and easy to operate The TLC plate cutter has a high-quality carbide scriber mounted into a movable plastic head. It is designed to produce optimal scoring on the glass surface of 0 x 0cm TLC plates with a total thickness (glass + coating) of mm. A smaller template is included with the unit for ease in cutting 0 x 0cm plates. TLC Plate Cutter Qty. TLC Plate Cutter ea 7535 Replacement Scribers ea 7565 Replacement Scribers tlc Multiple Plate Development Rack Size Anodized Aluminum Rack 0 x 0cm Plates 705 Anodized Aluminum Rack 0 x 0cm Plates 7053 Preval Sprayers Compressed Gas Sprayer Compressed Gas Sprayers provide a constant pressure and uniform distribution of fine mist on your TLC plate. The pressure unit contains difluoroethane. Unit sprays up to 6oz of liquid. Caution: Wetted parts are made of polypropylene or nylon. Do not store with dip tube immersed in reagent. Compressed Gas Sprayer Qty. Complete Spray Unit with Glass Jar Replacement Glass Jar with Lid Replacement Compressed Gas Unit Sample Applicators Uncalibrated Disposable Micropipettes Uncalibrated disposable glass micropipettes are intended for rapid qualitative sample application on TLC plates. Thick walls make these pipettes strong and easy to handle. Overall length is about three inches. Capacity is approximately 8µL. Uncalibrated Disposable Micropipettes Qty. Glass Micropipettes Glass Micropipettes Drummond Wiretrol Micropipettes Combines the accuracy of a microsyringe with the ease and economy of a disposable pipette Spraying Accessories Disposable Spray Box Heavy duty cardboard with disposal bag provided Drummond Wiretrol Micropipettes /Capacity Qty..0 to 5.0µL (Calibrated) TLC Spray Supports Qty. Disposable Spray Box

39 gc columns & accessories Grace GC Capillary Column Families Alltech Heliflex Column High-Performance, Widest Selection of Phases Alltech Econo-Cap Column Best Value, Popular Phases Grace offers two lines of capillary columns to suit every application and budget; Alltech Heliflex and Alltech Econo-Cap. Heliflex columns are Grace's premium line of individually tested capillary columns. Econo-Cap columns are batch tested to dramatically reduce the price without sacrificing quality. Both the Heliflex and Econo-Cap column lines are manufactured under identical conditions using the same high-quality polyimide coated fused silica and immobilized stationary phases. In addition each are mounted on a rugged cage. tech tip Our GC capillary columns are temperature rated and the test certificate that you receive with your column will indicate the minimum and maximum operating temperatures. In some cases we will list two maximum temperatures one for isothermal conditions and a higher one for temperature programmed method. Alltech Standard Phase Cross Reference gc columns Standard Phase Cross Reference Composition Alltech Phases USP Designation Similar Phases 00% Dimethylpolysiloxane AT -ms G, G, G38 DB-ms, HP-ms, Rtx -ms, CP-Sil 5CB low-bleed/ms phases 00% Dimethylpolysiloxane AT -ht, AT -, EC - G, G, G , CP-Sil 5CB, DB-, DB-ht, HP-, HP-0, OV -, RSL-50, RSL-60, Rtx -, SE-30, SPB -, SPB -Sulfur, ULTRA-, SP-00, BP-, DC-00, PE-, ZB- phases 5% Phenyl equivalent Polysilphenylene-Siloxane AT -5ms DB-5ms, Rtx -5 Sil ms, BPX -5, 007-5ms, HP-5ms phases (5% Phenyl)- 95% methylpolysiloxane (0% Phenyl)- 80% methylpolysiloxane (35 Phenyl)- 65% Methylpolysiloxane (50 Phenyl)- 50% methylpolysiloxane (6% Cyanopropylphenyl)- 94% methylpolysiloxane (4% Cyanopropylphenyl)- 86% methylpolysiloxane Trifluoropropylmethyl polysiloxane Polyethyleneglycol Polyethyleneglycol Acid Modified AT -5, EC -5 AT -0, EC -0 AT -35ms AT -35 G7, G36 G8, G3 G4 007-, CP-Sil 8CB, DB-5, DB-5.65, DB-5ht, HP-5, HP-5ms, OV -5, PTE-5, PTE- 5QTM, PAS-5, RSL-00, Rtx -5, Rtx -5ms, SAC-5, SE -54, SPB -5, ULTRA-, XTI-5, SE -5, BP-5, PE-, ZB-5 phases 007-7, Rtx -0, SPB -0, VOCOL, PE-7 phases DB-35ms, Rtx -35, SPB -35, SUP-HERB, MDN-35, BPX -35, 007-, DB-35, DB-35ms, HP-35, RSL-300, Rtx -35, SPB -35, SPB -608, OV -, PE-35, SUP- HERB phases AT -50 G3 DB-7ms, H5-50+, Rtx -50, SP-50, SPB -50, SPB -7, BPX -50, DB-7ht, Rtx -65TG, BPX -50, CP-TAB-CB, 007-7, DB-7, DB-7ht, HP-7, HP-50+, Rtx - 50, SP-50, SP-50, SPB -50, CP-Sil 4CB, PE-7, ZB-50 phases AT -64 G43 DB-64, HP-64, Rtx -64, SPB -64, , ZB-64 phases AT , CP-Sil 9CB, DB-70, HP-70, OV -70, PAS-70, Rtx -70, SPB -70, BP-0, ZB-70 phases AT -0 G6 DB-0, RSL-400, Rtx -00, OV -0, OV -0, OV -5, QF-, SP-40 phases AT -WAXms AT -WAX AT -AquaWax EC -WAX AT -000, EC -000, AT -AquaWax-DA G4, G5 G6, G0, G39 G5, G35 DB-WAX, Rtx -WAX, Supelcowax -0, 007-CW, Carbowax 0M, CP-Wax 5CB, DB-WAXetr, DB-WAX, Rtx -WAX, HP-0M, HP-Wax, Innowax, Omegawax, Stabilwax, Supelcowax -0, Superox II, BP-0, ZB-WAX phases 007-FFAP, CP-Wax 58CB, DB -FFAP, HP -FFAP, Nukol, OV -35, SP-000, Stabilwax -DA, Superox -FA, BP- phases Alltech Specialty Phase Cross Reference Specialty Phases Cross Reference Composition Alltech Phases Similar Phases EPA Method 50. AT , , DB-50., DB-VRX, HP-64, HP-VOC, MST-50., MXT-64, MXT-Volatiles, OV -64, Rtx -50., Rtx -64, Rtx -Volatiles, VOCOL phases 38

40 gc columns & accessories Heliflex Capillary Columns High-performance individually tested columns 7" rugged cage Most complete selection of phases Custom columns available to suit your specific application Heliflex capillary columns are manufactured from the highest quality polyimide coated synthetic fused silica. They are mounted on a rugged, wear-resistant cage. Each Heliflex column is individually tested to guarantee column performance and comes with a test chromatogram, instruction manual, capillary end caps and a free ceramic column cutter. premium Heliflex Standard Capillary GC Columns AT - AT -5 AT -0 AT -35 AT -70 AT -Wax AT -000 Length i.d. Film 0m 0.3mm 0.0µm m 0.53mm 0.5µm m 0.53mm.0µm m 0.53mm.65µm m 0.3mm 0.5µm m 0.3mm.00µm 370 5m 0.53mm 0.5µm m 0.53mm.00µm m 0.53mm.0µm m 0.53mm.50µm m 0.5mm 0.0µm m 0.53mm.0µm m 0.5mm 0.5µm m 0.5mm 0.50µm m 0.5mm.00µm m 0.3mm 0.5µm m 0.3mm 0.30µm m 0.3mm 0.50µm m 0.3mm.00µm m 0.3mm 3.00µm m 0.3mm 5.00µm m 0.53mm 0.50µm m 0.53mm.00µm m 0.53mm.0µm m 0.53mm.50µm m 0.53mm.00µm 30m 0.53mm.65µm m 0.53mm 5.00µm m 0.5mm 0.0µm m 0.3mm 0.30µm m 0.5mm 0.5µm m 0.5mm.00µm m 0.3mm 0.0µm gc columns (continued on next page) 39

41 gc columns & accessories Heliflex Capillary Columns (continued) Heliflex Standard Capillary GC Columns AT - AT -5 AT -Wax AT -000 Length i.d. Film 60m 0.3mm 0.5µm m 0.3mm 0.30µm m 0.3mm 0.50µm m 0.3mm 5.00µm m 0.53mm.00µm m 0.53mm 5.00µm 380 Heliflex AT -ms and HT Capillary GC Columns Ms grade columns are low bleed phases suitable for use with Mass spectrometers or other sensitive detectors AT -HT columns are rated to a higher maximum temperature for use with high boiling components gc columns Heliflex AT -ms and HT Capillary GC Columns AT -ms AT -HT AT -5ms AT -Wax ms Length i.d. Film 5m 0.5mm 0.0µm m 0.5mm 0.5µm m 0.5mm 0.0µm m 0.5mm 0.5µm m 0.5mm 0.5µm m 0.3mm 0.5µm 5896 *With 5m integral uncoated guard. AT Special Capillary GC Columns AT Special Capillary GC Columns AT -50 AT -0 AT -AquaWax AT -AquaWax-DA Length i.d. Film 30m 0.5mm 0.5µm m 0.3mm 0.5µm m 0.53mm 0.50µm m 0.53mm.00µm m 0.53mm.0µm m 0.5mm 0.5µm m 0.3mm 0.5µm

42 gc columns & accessories Other Heliflex AT Capillary GC Columns Other Heliflex AT Capillary GC Columns Phase Length i.d. Film AT m 0.3mm.80µm 3756 AT m 0.3mm 3.00µm 3780 AT m 0.53mm 3.00µm 6889 AT m 0.53mm 3.00µm 3800 AT m 0.53mm 3.00µm 3937 AT m 0.3mm.80µm 3790 AT m 0.53mm 3.00µm 3793 AT -Amino Acid 5m 0.53mm.0µm 6864 Custom Capillary Columns To inquire about the possibility of a custom capillary column, contact your local Grace office with the following information:. Stationary phase required. Length and Internal Diameter 3. Film Thickness required Alltech Econo-Cap Capillary Columns Best value in capillary columns Batch tested to dramatically reduce price Ideal for aggressive applications Available in the most popular phases We ve taken our most popular capillary columns and perfected the manufacturing procedure to the point that reproducibility is assured. This allows us to test these columns in batches instead of individually, while still offering a guarantee. Batch testing significantly reduces cost in production without sacrificing quality. value gc columns Each Econo-Cap capillary column comes with a copy of the batch test results, an instruction manual, and capillary end caps. Econo-Cap Capillary GC Columns EC - EC -5 EC -Wax EC -000 Length i.d. Film Qty. 5m 0.53mm.0µm ea m 0.5mm 0.5µm ea m 0.5mm 0.5µm 6/pk m 0.3mm 0.5µm ea m 0.3mm 0.5µm 6/pk m 0.53mm.0µm ea m 0.53mm.0µm 6/pk technical assistance Contact Tech Support: [email protected] Online: 4

43 gc columns & accessories gc columns GC Packed Columns Custom Packed Columns These columns are 6' x /8" o.d. 304 premium grade, precleaned stainless steel (unless otherwise indicated). Each column is made with preconditioned, pretested packing and comes complete with fittings, packing quality control chromatogram, column tags, and conditioning instructions. Popular GC Packed Columns Column Dimensions Alumina F, 60/80 5' x /8" SS Tubing 5664PC 0% Carbowax 0M on Chromosorb 6' x /8" SS Tubing 005PC W-AW, 80/00 0% Carbowax 0M on Chromosorb 6' x /8" SS Tubing 06PC W-HP, 80/00 0.0% Carbowax 500 on Graphpac - 6' x /8" SS Tubing 8546PC GC, 80/00 Chromosorb 07, 80/00 6' x /8" TFE Tubing 9783 Chromosorb 750, 80/00 4' x /8" SS Tubing 4480 Gas Chrom 0, 80/00 6' x /8" SS Tubing 484PC Hayesep D, 80/00 6' x /8" SS Tubing 4487 Hayesep D, 00/0 0' x /8" Ni Tubing 708PC Hayesep D, 00/0 30' x /8" SS Tubing 7083PC Hayesep D, 00/0 0' x /8" SS Tubing 830PC Hayesep DB, 00/0 30' x /8" SS Tubing 836PC Hayesep Q, 80/00 8' x /8" SS Tubing 800PC Hayesep Q, 80/00 6' x /8" SS Tubing 80PC Hayesep Q, 00/0 6' x /8" SS Tubing 4489 Hayesep P, 60/80 8' x /8" SS Tubing 803PC Hayesep P, 80/00 6' x /8" SS Tubing 804PC Hayesep T, 60/80 3' x /8" SS Tubing 449 Hayesep T, 80/00 6' x /8" SS Tubing 83PC Molecular Sieve 5A (washed), 60/80 6' x /8" SS Tubing 4494 Molecular Sieve 5A (washed), 80/00 6' x /8" SS Tubing 5605PC Molecular Sieve 5A (washed), 80/00 0' x /8" SS Tubing 4495 Molecular Sieve 3X (washed), 80/00 3' x /8" SS Tubing 449 Molecular Sieve 3X (washed), 80/00 6' x /8" SS Tubing 5773PC Molecular Sieve 3X (washed), 80/00 0' x /8" SS Tubing 4493 Porapak P, 80/00 6' x /8" SS Tubing 4499 Porapak Q, 60/80 6' x /8" SS Tubing 700PC Porapak Q, 80/00 ' x /8" SS Tubing 70PC Porapak Q, 80/00 6' x /8" SS Tubing 70PC Porapak Q, 00/0 6' x /8" SS Tubing 70PC Porapak QS, 80/00 6' x /8" SS Tubing 79PC Porapak T, 80/00 6' x /8" SS Tubing 73PC 0% SE-30 on Chromosorb W-HP, 6' x /8" SS Tubing 43PC 80/00 Silica Gel Grade, 60/80 8' x /8" SS Tubing 565PC Silica Gel Grade, 80/00 8' x /8" SS Tubing % Silar 0C on Chromosorb W-HP, 6' x /8" SS Tubing 430PC 00/0 Tenax TA, 60/80 4' x /8" SS Tubing 4538 Tenax TA, 60/80 6' x /8" SS Tubing 4900PC Tenax TA, 80/00 6' x /8" SS Tubing 490PC Unibeads S, 60/80 6' x /8" SS Tubing 760PC Unibeads 3S, 60/80 6' x /8" SS Tubing 454 0% Silicone UCW-98 on Chromosorb W-HP, 80/00 6' x /8" SS Tubing 8493PC Custom Packed GC Columns are available in a variety of tubing types including stainless steel, PTFE, and Deactiglas tubing (silane treated glass). A (Arm ) Contact your local Grace office to request a quote. Please include your desired tubing, stationary phase, loading, and support. We may request S (Span) additional information about your instrument or dimensions of existing columns. A (Arm ) A (Arm ) A (Arm ) A A (Arm (Arm ) ) A (Arm A ) (Arm ) A (Arm ) A (Arm ) A (Arm ) Alltech CTR Concentric S (Span) Packed Columns A (Arm ) S (Span) S (Span) S (Span) S (Span) For Gas Analysis CTR I is ideal for separating O, N, CH4, CO, and CO at ambient conditions CTR III is ideal for argon analysis in the presence of O Useful where two separate chromatographic runs are required to analyze one sample Individually tested A CTR column is essentially a column within a column. This permits you to use two different packings for the analysis of your sample. The diagram shows the construction of the CTR column. Inner /8" o.d. Tube Containing Column Packing A Outer /4" o.d. Tube Containing Column Packing B Cut-away view of CTR column. technical assistance Contact Tech Support: [email protected] Online: CTR Columns Temp. Limit CTR III Argon Analysis Column 75 C 875 4

44 gc columns & accessories GC Packings and Supports Pretested GC Packings Temp. Limit Qty. Alumina F-, 60/ C 00g 5664 % AT -000 on Carbograph, 60/80 50 C 5g 733 5% Carbowax 0M on Chromosorb W-HP, 60-5 C 0g /00 0% Carbowax 0M on Chromosorb 60-5 C 0g 005 W-AW, 80/00 0% Carbowax 0M on Chromosorb 60-5 C 0g 06 W-HP, 80/00 Molecular Sieve 5A (washed), 60/ C 50g 5604 Molecular Sieve 5A (washed), 80/ C 50g 5605 Molecular Sieve 3X (washed), 60/ C 50g Molecular Sieve 3X (washed), 80/ C 50g % OV - on Chromosorb W-HP, 80/ C 0g 76 3% OV -7 on Chromosorb W-HP, 00/ C 0g 07 3% OV -7 on Chromosorb W-HP, 80/ C 0g 79 Porapak PS, 00/0 50 C 5g 64 Porapak Q, 00/0 50 C 5g 68 Silica Gel Grade, 60/ C 00g 565 Silica Gel Grade, 80/ C 00g 5653 Tenax TA, 60/ C 0g 0496 Tenax TA, 80/ C 0g 0497 Tenax GR, 60/ C 0g 4937 Unibeads S, 60/80 00 C 5g 75 Unibeads S, 60/80 00 C 5g 760 Unibeads 3S, 60/80 00 C 5g 764 Unibeads 3S, 80/00 00 C 5g 766 Pretested packings are supplied with a test chromatogram. Custom Coated Packings To request a quote on a custom coated packing, contact your local Grace office with the following information: Please call us with:. Solid support required. Mesh size 3. Stationary phase and % loading GC Supports Chromosorb Diatomite Supports Mesh Qty. Chromosorb A-NAW 0/30 454g 43 Chromosorb P-NAW 30/60 454g /60 454g /80 454g /00 454g /0 454g 308 Chromosorb P-AW 30/60 454g 34 60/80 454g 36 80/00 454g 37 Chromosorb P-AW-DMCS 80/00 454g 35 00/0 454g 36 Chromosorb W-NAW 30/60 50g /60 50g /00 50g 336 Chromosorb W-AW 30/60 50g /80 50g 347 Chromosorb W-AW-DMCS 80/00 50g 360 Chromosorb W-HP 80/00 50g /0 50g 366 Chromosorb 750 (AW-DMCS) 80/00 00g 400 Chromosorb T (Non-Diatomite) 30/60 5g 437 Chromosorb Diatomite Supports Specifications Chromosorb Diatomite Supports Series Surface Area Packed Density Recommended Liquid Phase Load Type A Flux-calcined.7m /g 0.48g/cc 5% G Flux-calcined 0.5m /g 0.58g/cc 5% P Calcined 4.0m /g 0.47g/cc 30% W Flux-calcined.0m /g 0.4g/cc 5% 750* Flux-calcined 0.75m /g 0.40g/cc % T** PTFE 7 8m /g 0.4g/cc % *Chromosorb 750 support material is the most inert and highly efficient in the series. This support material is acid-washed, DMCS treated, and carefully screened. **Chromosorb T is a non-diatomite support useful for the analysis of polar compounds such as water, hydrazine, sulfur dioxide, etc. Chromosorb Diatomite Supports Treatment Specifications Abbreviation Treatments NAW Untreated (non-acid washed) AW Acid washed (HCl is used) DMCS Dimethyldichlorosilane (silanized) HP High performance QC tested (acid washed, silanized, flux-calcined) gc packings & supports 43

45 gc columns & accessories GC Supports Molecular Sieves Molecular sieves are synthetic alkali metal alumino-silicates with various cations. They are used for the separation of fixed gases and drying of liquid or gas streams. They can be reactivated by heating at 50 C for hours; 300 C for 4 hours; or 350 C for hours. The washed molecular sieves offered here have been carefully cleaned of dust particles by washing with distilled water. The cleaned product is then activated and sealed in glass jars. Molecular Sieves Mesh Temp. Limit Qty. Molecular Sieve 3A (For Drying Applications) /6" Pellets 350 C 00g / C 00g / C 00g /0 350 C 00g Molecular Sieve 4A (For Drying Applications) 8/ Beads 350 C 00g 0556 /6" Pellets 350 C 00g / C 00g 56 80/ C 00g 564 Molecular Sieve 5A (For Drying Applications) /8" Pellets 350 C 00g 0564 /6" Pellets 350 C 00g / C 00g 5630 Molecular Sieve 3X (For Drying Applications) 8/ Beads 350 C 00g 0568 /6" Pellets 350 C 00g (KG) 057 (0573) 40/ C 00g / C 00g / C 00g 5638 Washed Molecular Sieve 5A (For Chromatography Applications) 40/ C 50g / C 50g / C 50g 5605 Washed Molecular Sieve 3X (For Chromatography Applications) 40/ C 50g / C 50g / C 50g /0 300 C 50g gc packings & supports GC Supports Other GC Supports GC Supports Mesh Temp. Limit Qty. Comments T-Port-F 80/00 50 C 50g 449 TFE support for very polar compounds. Activated Alumina F- 60/ C 00g 5664 Useful for light hydrocarbon analyses. 80/ C 00g 5666 Useful for light hydrocarbon analyses. Glass Beads Regular 60/80 5g 540 Can support loadings of up to 0.5%. 00/0 5g 544 Can support loadings of up to 0.5%. Glass Beads DMCS Treated 80/00 5g 548 Can support loadings of up to 0.5%. 00/0 5g 5430 Can support loadings of up to 0.5%. Silica Gel Grade 40/60 00g (KG) 5650 (0553) Used for analysis of fixed gases and light hydrocarbons. 60/80 00g (KG) 565 (05533) Used for analysis of fixed gases and light hydrocarbons. 80/00 00g (KG) 5653 (05534) Used for analysis of fixed gases and light hydrocarbons. 00/0 00g (KG) 5655 (05535) Used for analysis of fixed gases and light hydrocarbons. Silica Gel Grade 5 35/60 00g (KG) 5648 (05545) Used for analysis of fixed gases and light hydrocarbons. Unibeads S Å 60/80 5g 75 Spherical porous polymer beads. 80/00 5g 758 Spherical porous polymer beads. Unibeads S 60Å 60/80 5g 760 Comparable to Porasil A. 80/00 5g 76 Comparable to Porasil A. Unibeads 3S 00Å 60/80 5g 764 Comparable to Porasil B. 80/00 5g 766 Comparable to Porasil B. 44

46 gc columns & accessories GC Supports Graphitized Carbon Blacks Graphitised Carbon Blacks Mesh Temp. Limit Qty. Comments Carbograph 60/80 >500 C 0g 7 Equivalent to Carbopack B Support. 80/0 >500 C 0g 74 Equivalent to Carbopack B Support. Carbograph -TD 0/40 >500 C 5g 745 Equivalent to Carbopack C Support Suitable for thermal desorption work. Carbograph 60/80 >500 C 0g 76 Equivalent to Carbopack C Support. 80/00 >500 C 0g 78 Equivalent to Carbopack C Support. Carbograph -SC 40/60 5 C 0g 734 Similar to Carbopack BHT Support. Graphpac -GC Uncoated 60/80 5g 8536 Average surface area of 0-3 m /g Unique separation properties. GC Supports Porous Polymers Porous Polymers Mesh Temp. Limit Qty. Tenax TA (GC) 0/ C 0g / C 00g / C 0g / C 00g / C 0g / C 00g / C 500g / C 0g / C 00g 0497 Tenax GR 0/ C 0g / C 00g / C 0g / C 0g / C 500g / C 0g 4939 Hayesep A 80/00 65 C 75cc 89 00/0 65 C 75cc 80 0/40 65 C 75cc 8 Hayesep D 60/80 90 C 75cc 88 80/00 90 C 75cc 89 00/0 90 C 75cc 830 Hayesep DB 00/0 90 C 75cc 836 Hayesep N 80/00 65 C 75cc 86 Hayesep P 60/80 50 C 75cc 803 Hayesep Q 60/80 75 C 75cc /00 75 C 75cc 80 Hayesep R 60/80 50 C 75cc /00 50 C 75cc 807 Hayesep T 80/00 65 C 75cc 83 Porapak Q 50/80 50 C 6g /00 50 C 6g 70 00/0 50 C 6g 70 Porapak P 50/80 50 C 0g /00 50 C 0g 704 Porapak R 80/00 50 C 4g /0 50 C 4g 708 Porapak S 50/80 50 C 6g /00 50 C 6g 70 Porapak T 80/00 90 C 3g 73 Porapak N 50/80 90 C 9g 75 80/00 90 C 9g 76 00/0 90 C 9g 77 Porapak PS 80/00 50 C 0g 7 00/0 50 C 0g 73 GC Supports Chromosorb Century Series Porous Polymers Chromosorb 0 60/80 75/35 C* 50g 405 Chromosorb 0 80/00 50/300 C* 50g 409 Chromosorb 06 60/80 50/75 C* 50g 40 Chromosorb 07 80/00 50/75 C* 50g 44 *Isothermal/temp. program. Tenax -TA (GC) Polymer Tenax -TA porous polymer is based on,6-diphenyl-pphenylene Oxide. It has replaced Tenax -GC polymer and can be used as a packing and as a trapping material. Both the EPA and NIOSH specify the use of Tenax polymer in their standard methods. It is particularly useful for the analysis of high boiling compounds such as alcohols, polyethylene glycols, diols, phenols, monoamines and diamines, ethanolamines, aldehydes, ketones and chlorinated aromatics. Tenax -GR Polymer Tenax -GR polymer contains 3% graphitized carbon as an integral part of the material. This is not an admixture; the graphitized carbon is co-precipitated with the Tenax polymer. The resulting material gives higher breakthrough volumes for most materials, yet is less water retentive than Tenax -TA polymer. When using this material for packing GC columns, better peak symmetry is observed. Surface area is 4.m /g. HayeSep Porous Polymer Pre-purified porous polymers for GC Less bleed and shrinkage than other porous polymers Cleaned and conditioned to 00 C (65 C for N & T) HayeSep polymers were developed to solve room temperature separation of N, O, Ar and CO; ppm HO in the presence of HCl, Cl, etc. The HayeSep D polymer series better separates the fixed gases to the C hydrocarbons. Porapak Packings Porapak GC packings are cross-linked polymers that can be used directly in GC columns without a stationary phase coating. Acetone washing of Porapak GC packings improves their performance. Porapak GC Packing Specifications Series Polymer Composition Surface Area Polarity Q DVB*/Ethylvinylbenzene m /g Slightly Polar P DVB*/Styrene 00 00m /g Non-Polar R DVB*/Vinyl pyrrolidinone m /g Mod. Polar S DVB*/Vinyl pyridine m /g Mod. Polar T EGDM** 5 350m /g Polar N DVB*/Vinyl pyrrolidinone m /g Very Polar *Divinylbenzene. **Ethyleneglycoldimethacrylate. gc packings & supports 45

47 gc columns & accessories Septa Chromatogram Cross Reference Guide Actual Size Drawing Measurement /4" (6mm) Shimadzu*, SRI* Chromatographs 3/8" (9.5mm) 7/6" (mm) Antek, Finnigan 900, 9600, Gow- Mac, Agilent 5700, 5880 & 5900, IBM, Packard , Pye-Unicam (early models), Varian (except capillary injector), Tracor 550, 560 Chromatographs Carle, Agilent 5890,6850,6890. P-E Sigma, 900, 990, 390, 8300, 8400, 8500 Autosys, Varian (capillary injector), SPI, Varian 078/079 Chromatographs Microsep Low-Bleed PTFE-Lined Septa Maximum temperature of 50ºC Off-white silicone septa with yellow PTFE backing /" (.5mm) Fisons/Carlo Erba, Finnigan 9500, Agilent 5750 and earlier models, Packard 409-4, Tracor 0,, 540 Chromatographs /3" (7mm) Fisons/Carlo Erba 8000 Series, CE TRACE GC Chromatographs Microsep Low-Bleed PTFE-Lined Septa Size (o.d.) Qty. 3/8" (9.5mm) x /6" (mm) x /" (.5mm) x *Use cylindrical plug septa. gc accessories General Purpose Blue Septa Maximum temperature of 50 C Lifetime of up to 50 injections Suitable for most GC analysis General Purpose Blue Septa Size (o.d.) Qty. /4" (6mm) /8" (9.5mm) /6" (mm) /" (.5mm) " x " Sheet ea 658 XLB Septa Maximum temperature to 350 C Extremely low bleed Superior resealing properties Soft, easily penetrated by syringe needle XLB Septa Size (o.d.) Qty. /4" (6mm) /8" (9.5mm) /6" (mm) /" (.5mm) /3" (7mm) ThermoRed Septa Maximum temperature of 300 C Packed in glass jars under strict QC standards to protect from contaminants ThermoRed Septa Size (o.d.) Qty. /3'' (9mm) /8'' (9.5mm) /6'' (mm) 5 53 /" (.5mm) 5 54 /3'' (7mm) Plug Type (For Shimadzu and SRI) HT-X High-Performance Septa Maximum temperature of 350 C Long injection life over 00 injections HT-X High-Performance Septa Size (o.d.) Qty. 3/8" (9.5mm) /6" (mm) /3" (7mm) /" (.5mm) " x " Sheet ea

48 gc columns & accessories GC Ferrule Selection Ferrule Properties Material Temp. Limit Reusability Resealing Properties Suitability for Glass Temp. Prog. PTFE 50 C Good Excellent Excellent No Graphite 450 C Good Excellent Excellent Yes Vespel 350 C+ Excellent Good Good Yes VG 400 C Excellent Good Excellent Yes VG 400 C Excellent Good Excellent Yes What ferrule type do you need? Ferrule Type Use for 0.4mm Hole: Capillaries with i.d. of mm 0.5mm Hole: Capillaries with i.d. of mm 0.8mm Hole: Capillaries with i.d. of mm NO-HOLE: As plugs, or drill for special needs. PTFE and Graphite Ferrules Fitting Size Tubing Size Qty. PTFE Graphite Straight Ferrules " " 436 7/8" 7/8" /4" 3/4" /8" 5/8" 4357 Used when /" /" 0 SF-800-G the tubing o.d. 3/8" 3/8" 0 SF-600-T SF-600-G matches the nominal size /4" /4" 0 SF-400-T SF-400-G of the fitting 3/6" 3/6" 0 SF-300-T SF-300-G being used. /8" /8" 0 SF-00-T SF-00-G /6" /6" 0 SF-00-T SF-00-G Reducing Ferrules /4" /6" 0 RF-400/00-T RF-400/00-G /4" /8" 0 RF-400/00-T RF-400/00-G /4" 3/6" 0 RF-400/300-T 437 /8" /6" 0 RF-00/00-T RF-00/00-G /8".0mm 0 43 /8" 0.8mm 0 RF-00/0.8-G /8" 0.5mm 0 RF-00/0.5-G Used when /8" 0.4mm 0 RF-00/0.4-G the tubing o.d. /6".0mm 0 RF-00/.0-G is smaller than /6" 0.8mm 0 RF-00/0.8-G the nominal size of the fittings. /6" 0.5mm 0 RF-00/0.5-G /6" 0.4mm 0 RF-00/0.4-G No-Hole Ferrules 3/8" /4" 0 RF-400/0-T 438 /8" 0 RF-00/0-T 434 /6" 0 RF-00/0-T 430 Graphite Ferrules for HP/Agilent Nuts 0.5mm Hole mm Hole gc accessories Vespel and Vespel /Graphite Ferrules Fitting Size Tubing Size Qty. Straight Ferrules Reducing Ferrules Vespel VG /4" /4" 0 SF-400-V SF-400-VG /8" /8" 0 SF-00-V SF-00-VG /8" /8" /6" /6" 0 SF-00-V SF-00-VG /4" 3/6" 0 RF-400/300-V 400/300-VG /4" /8" 0 RF-400/00-V 400/00-VG /4" /6" 0 RF-400/00-V 400/00-VG /4" 0.8mm 0 400/0.8-VG /8" /6" 0 RF-00/00-V 00/00-VG /8" 0.8mm 0 00/0.8-VG /8" 0.4mm 0 00/0.4-VG /6" 0.9mm 0 RF-00/0.9-V 00/0.9-VG /6" 0.8mm 0 RF-00/0.8-V 00/0.8-VG /6" 0.5mm 0 RF-00/0.5-V 00/0.5-VG /6" 0.4mm 0 RF-00/0.4-V 00/0.4-VG /6" 0.3mm 0 00/0.3-VG 47

49 gc columns & accessories Swagelok Tube Fittings Swagelok tube fittings provide a leak-proof, torque-free seal at all tubing connections and eliminate costly, hazardous leaks in instrumentation and process tubing. tech tip CAUTION: Do not mix or interchange parts of tube fittings made by other manufacturers with Swagelok tube fitting parts. All the action in the fitting moves along the tube axially instead of with a rotary motion. Since no torque is transmitted from the fitting to the tubing, there is no initial strain which might weaken the tubing. Swagelok Fittings Dimensions Qty. Nuts Brass Qty. Stainless Steel /6" /8" /4" Front Ferrules Back Ferrules Crosses /6" /8" /4" /6" /8" /4" /8" ea 5503 ea 553 /4" ea 5503 ea 553 gc accessories Parker Fittings Most parts are available in brass or stainless steel and should be matched to the tubing, particularly in thermal applications. Parker Fittings Dimensions Qty. Nuts Ferrules Tees Unions Reducing Unions CPI Brass Qty. CPI Stainless Steel /6" 0/pk /pk /8" 0/pk /pk 458 /4" 0/pk /pk 459 /6" 0/pk /pk 4605 /8" 0/pk 406 0/pk 46 /4" 0/pk 406 0/pk 46 /6" ea 4075 ea 475 /8" ea 4053 ea /4" ea 4077 ea 477 /6" ea 4063 ea 463 /8" ea 4065 ea 465 /4" ea 4068 ea 468 /8" to /6" ea 4064 ea 464 /4" to /6" ea ea 466 /4" to /8" ea 6008 ea 608 3/8" to /4" ea ea

50 gc columns & accessories Moisture Traps Remove Moisture from Your Gas System Eliminate stationary phase degradation Increase column life Remove moisture from gas cylinders, regulators and gas lines with a Grace moisture trap. In addition to removing HO, most traps (except the ALL-Pure moisture trap and the Sodium Sulfate moisture trap) also remove HCl, SO, NO, HS, CO, oil and small aliphatics. Both indicating and non-indicating traps are available. Many traps can be regenerated and are refillable order refill kits separately. In-Line Style Traps* Capacity Efficiency Standard Moisture Trap Hydro-Purge II Trap Sodium Sulfate Moisture Trap *Length is measured from endfitting to endfitting. **Capacity based on 50% efficiency. These traps are disposable. Oxygen Traps ml 3mL ml 36mL <ppm <5ppb <0ppb Pressure Limit 00psig 5psig Glass Moisture Trap 6mL <0ppb 5psig ALL-Pure Moisture Trap 35mL <ppb 000psig Fitting Dimensions (L x o.d.) Indicator Refill Kit /8" " x.5" /4" Yes /8" " x " /4" /8" " x.5" /4" Yes /8" " x.5" /4" /8" " x.5" Yes /4" /8" " x.5" No /4" 7036 High-Pressure Gas Drier 7mL <ppm 800psig /4".5" x.5" No 83 BIG Moisture Trap 30mL <5ppb 50psig Protect Columns from Oxygen Contamination /8" 707 /4" 7.5" x.4" No 709 /" mL** <ppm 00psig /4" 9.0" x.5" Yes Prevent oxidation of column stationary phases with Grace oxygen traps. Use an oxygen trap to help maintain column performance and prolong column life. Choose an indicating trap to easily identify when trap is spent or to use in conjunction with larger capacity traps. gc accessories In-Line Style Traps Capacity Efficiency Pressure Limit Fitting Dimensions (L x o.d.) Indicator /8" 400 Oxy-Trap 33mL <ppb 00psig /4" 3.5" dia coil No 400 Large Oxy-Trap 06mL <ppb 00psig /4" 3-7/8" dia coil No 4003* Indicating Oxy-Trap 0mL <ppb 00psig /4"* 6" x.375" Yes 4004* Indicating Oxy-Purge 50mL <3ppb 00psig /8" " x.5" Yes /4" 456 Oxy-Purge N Trap 56mL <30ppb 50psig /8" 4636 " x.5" No /4" 4635 /8" 73 BIG Oxygen Trap 3000mL <50ppb 50psig /4" 7.5" x.4" No 75 /" 77 High-Pressure Oxy- Trap 456mL <ppb 800psig /4".5" x.5" No 85 *Reducing ferrules are supplied for installation into /8" lines. 49

51 gc columns & accessories Hydrocarbon, CO and Sulfur Traps Eliminate Detector Noise and Increase Baseline Stability Grace hydrocarbon traps adsorb trace amounts of hydrocarbons and organics from your gas stream. Most traps are packed with activated charcoal and adsorb organic compounds larger than methane. A) Alltech High-Pressure CO Adsorber 800psig pressure limit 7mL capacity Also removes NO B) Sulfur Purifier Also removes moisture, oxygen, C and above hydrocarbons, halocarbons and fluoro lubricants In-Line Style Traps* Size Efficiency Pressure Limit Fitting Dimensions (L x o.d.) Indicator Refill Kit Activated Charcoal Trap 0cc 3" x.5" <00ppb 00psig /4" No 400cc 4.5" x.0" 83 Hydrocarbon Trap 00cc <0ppb 50psig /8" " x.5" No /4" 4633 Hi-EFF Organic Trap 400cc <00ppb 00psig /4" 4.5" x " Yes (for HO only) /8" BIG Hydrocarbon Trap 750cc <5ppb 50psig /4" 7.5" x.4" No 703 /" 705 ALL-Pure Hydrocarbon Trap <3ppb 000psig /8" " x.5" No /4" 7038 High-Pressure Charcoal Trap 00cc <00ppb 800psig /4".5" x.5" No 87 A High-Pressure CO Adsorber 00cc 800psig /4".5" x.5" No 89 B Sulfur Purifier 50cc <ppb 300psig /8" " x.5" No 7006 *Efficiency applies to compounds >C4. Length is measured from endfitting to endfitting. These traps are disposable. gc accessories ALL-Pure Gas Purifiers One purifier removes multiple contaminants Replace three separate purifiers for oxygen, moisture, and hydrocarbons with a single ALL-Pure purifier ALL-Pure Gas Purifier Specifications Dimensions: 400cc, " L x.5" o.d. (50.8cm x 3.8cm) Max. Flow: 500mL/min Max. Pressure: 000psig Max. Temp: 80 C Mounting: Purifier must be mounted in vertical position. Purifier Selectivity and Purity Specifications* Purifier CO CO O HO Sulfur Compounds Non-Methane Hydrocarbons Helium Purifier: < < < < < <3 Hydrogen Purifier: < < < < < <3 Nitrogen Purifier: < < < < < <3 Air Purifier: < <3 Moisture Trap: < < Hydrocarbon Trap: <3 Oxygen Trap: < < *Outlet levels listed indicate ppb concentration based on 50ppm nominal inlet concentration level. ALL-Pure Gas Purifiers /8" Fitting /4" Fitting Helium Purifier Hydrogen Purifier Nitrogen Purifier Air Purifier Alltech Gas Purification Cartridge Systems Replace spent purifiers without shutting down your system Alltech gas purification cartridges allow replacement of spent cartridges in seconds without shutting down your GC system. Simply pull the spent cartridge off the baseplate and push a new one into place. A knurled locking ring keeps the cartridge locked in place. Four different purification cartridges are available. Multiple cartridges and baseplates can be plumbed together to customize a gas purification system for your application. Order each cartridge separately below. Knurled locking ring Convenient cartridge baseplate automatically stops gas flow when changing cartridges Gas Purification Cartridge Systems* Cartridge System Base Unit** 805 Moisture Trap Cartridge 8005 Organic Trap Cartridge 800 AT -Omni Cartridge 80 AT -Indicating Oxy Cartridge 805 *Baseplate must be purchased separately. **Reducing ferrules are supplied for installation into /8" gas lines. Includes /4" fittings. Removes oxygen, moisture, and hydrocarbons. Removes oxygen and small amounts of moisture. 50

52 gc columns & accessories Deactivated Injection Liners Deactivated Injection Liners and Length x o.d. Injection Liners for Agilent Instruments* Cup Splitter, 78.5 x 6.3mm Split/Splitless with Deactivated Quartz Wool, 78.5 x 6.3mm o.d., 4mm i.d. Splitless, 78.5 x 6.3mm o.d., 4mm i.d. Quartz Splitless, 78.5 x 6.0mm, mm i.d. Recessed Gooseneck with Deactivated Quartz Wool, 78.5 x 6.3mm, 4mm i.d. Recessed Gooseneck, 78.5 x 6.3mm, mm i.d. Direct, 78.5 x 6.3mm,.5mm i.d. Injection Liners for Varian Instruments Splitter with Deactivated Quartz Wool, 075/077, 7 x 6.3mm, 4mm i.d. Baffle Splitter, 075/077, 7 x 6.3mm, 4mm i.d. 0.8mm SPI 093/094, 54 x 4.6mm 0.5mm SPI 093/094, 54 x 4.6mm Injection Liners for PerkinElmer Instruments Split/Splitless Autosystem, 9 x 5.8mm, 4mm i.d. Injection Liners for Shimadzu Instruments Split 7A with Deactivated Quartz Wool, 95.5 x 5.0mm, 3mm i.d. Splitless 7A, 95.5 x 5.0mm, 3mm i.d. Deactivated SGE FocusLiners Deactivated SGE FocusLiners and Length x o.d. for Agilent Instruments* FocusLiner, 78.5 x 6.3 x 4mm i.d. Tapered FocusLiner, 78.5 x 6.3 x 4mm i.d. for Varian Injector Models 078/079 FocusLiner, 54 x 5 x 3.4mm i.d. Tapered FocusLiner, 54 x 5 x 3.4mm i.d. for Shimadzu 7A FocusLiner, 95 x 5 x 3.4mm i.d. Tapered FocusLiner, 95 x 5 x 3.4mm i.d. for Shimadzu 4/5A/6 Tapered FocusLiner, 99 x 5 x 3.4mm i.d. for PerkinElmer Autosystem FocusLiner, 86. x 4 x mm i.d. FocusLiner, 9 x 6. x 4mm i.d. Tapered FocusLiner, 9 x 6. x 4mm i.d. for ThermoQuest Model 8000/TRACE FocusLiner, 05 x 8 x 5mm i.d. *Also compatible with Varian model CP-77 Split/Splitless injection port. best value Diagram Diagram 5/pk 5/pk /pk 5/pk gc accessories 5

53 gc columns & accessories Gas Sampling Bags ALTEF Gas Sampling Bags High tensile strength resists puncture Low permeability minimizes sample loss ALTEF is a fluoropolymer that is an alternative to Tedlar for gas sampling applications FEP Gas Sampling Bags FEP bags have a 5mil film thickness making them suitable for harsh sampling conditions Bags are chemically resistant to the most severe corrosives ALTEF and FEP Gas Sampling Bags ALTEF Gas Sampling Bags FEP Gas Sampling Bag Nominal Size Volume Dual On-Off/Septum Port /4" SS Swagelok Dual On-Off/Septum Port /4" SS Swagelok 6" x 6" 0.6L " x 7".0L " x 9" L " x " 5L " x 9" 0L " x 8" 6L " x 4" 40L " x 30" 56L " x 30" 80L gc accessories Combo Valve Gas Sampling Bags On/off valve and septum in one fitting Save with convenient multi-packs best seller Cali-5-Bond 5-Layer Gas Sampling Bags Non-permeable Opaque to UV and visible light Inert, flexible, tough, and reusable Combo valve gas sampling bags give the convenience and ease of a barbed on/off valve and a septum port combined into one fitting. The valve fitting features a 3/6" stem, which is suitable for use with most /4" tubing, and a sampling port. Bags are available in ALTEF and FEP. Combo Valve Gas Sampling Bags Size Volume Port ALTEF Gas Sampling Bags 7 x 7" L N x 9" L N x " 5L N x 9" 0L N x 4" 5L N FEP Gas Sampling Bags 6 x 6" 0.6L N x 0".L N x ".5L N x 9" L N 4008 Cali-5-Bond gas sampling bags are constructed from inert 5.5mil thick material made from five layers of film. The layers are comprised of an inner layer of high-density polyethylene, followed by polyamide, an aluminum foil barrier, a polyvinylidene chloride layer and an outer layer of polyester. Bags are equipped with a barbed on-off valve and silicone septum for easy sampling and can be purged with nitrogen or pure air gas and reused. 5-Layer Gas Sampling Bags Nominal Size Volume Port Snout-Shaped Gas Sampling Bags 8 x 5" L Barbed Valve " Septa x 7" 5L Barbed Valve " Septa 45 6 x 6" 0L Barbed Valve " Septa 486 x 4" L Barbed Valve " Septa 454 Pillow-Shaped Gas Sampling Bags 8 x 8" L Barbed Valve " Septa x " L Barbed Valve " Septa x 9" 5L Barbed Valve " Septa 49 5

54 spe and filtration SPE Introduction SPE method development typically contains four steps: Step : Condition The conditioning step is composed of two substeps; the first activates the sorbent ligands, the second equilibrates the sorbent bed. Step : Load In the load step, sample is applied to the SPE device. Matrix and flow rate are optimized to quantitatively retain target analytes. Step 3: Wash In the wash step, choose a solvent that elutes impurities but retains target analytes. Often the second conditioning solvent is a suitable wash solvent. Step 4: Elute The elution step ideally removes all target analytes with minimal solvent to maximize sensitivity. Sometimes this requires a combination of solvents to break both the primary and secondary interactions. Reversed-Phase Extraction Procedure Mechanism: Bind moderately polar to non-polar compounds from a polar sample matrix. Normal Phase Extraction Procedure Mechanism: Bind polar compounds from a non-polar sample matrix. Ion-Exchange Extraction Procedure Mechanism: Bind charged (negative/anionic or positive/cationic) compounds. General Method Development Procedures Step Condition ~ 4 bed volumes Step Load Step 3 Wash ~ 6 bed volumes Methanol followed by water IPA followed by hexane Methanol:water (50:50) followed by low ionic strength (0.M) buffer Process sample at a flow rate of 5mL/min Process sample at a flow rate of 5mL/min Apply slowly: less than or equal to ml/min ion exchange kinetics are slower than reversedor normal phase Water or water:methanol (95:5) Hexane or hexane:ipa (98:) Methanol:low ionic strength (0.M) buffer (0:90) Step 4 Elute ~ 3 bed volumes Methanol or acetonitrile. May need to add strong acid or base to organic solvent to break secondary interactions. IPA, ethyl acetate, acetone, or hexane:ipa (50:50) High ionic strength (0.5M.0M) buffer or modify ph such that the analyte is uncharged. May need to add organic to break hydrophobic interactions. spe tech tip To calculate sorbent bed volume, use 50µL for every 00mg of sorbent. tech tip Retention capacity describes the total amount that an SPE sorbent will bind. This includes all compounds retained analytes of interest as well as the contaminants. tech tip The minimum elution volume recommended in the bed size chart above will offer best sensitivity, but more solvent may be required depending on the application. Solvent Polarity Chart Less Polar (Stronger Reversed-Phase Elution) Hexane Isooctane Cyclohexane Carbon tetrachloride Toluene Chloroform Methylene chloride Tetrahydrofuran Ethyl ether Acetone Acetonitrile Pyridine Isopropanol Ethanol Methanol Water Acetic acid More Polar (Stronger Normal Phase Elution) 53

55 spe and filtration SPE Sorbent Specifications Reversed-Phase (Non-Polar) Sorbent Specifications % Carbon Endcapped Average Particle Size Pore Size Features Benefits Packing Base Prevail C8 Silica.0% Yes 50µm 60Å 00% water wettable Hydrophilic/hydrophobic retention. Phase remains active even when completely dry. Can omit preconditioning step. Standard C8 Silica 6.0% Yes 50µm 60Å Low carbon load C8 General purpose phase. High-Flow C8 Silica 8.0% Yes 00µm 60Å Large particle Less flow resistance for faster flow rates of large volume sample. High-Capacity C8 Silica 7.0% Yes 50µm 60Å High carbon load Maximum capacity phase. Large Pore C8 Silica 4.0% Yes 50µm 50Å Larger than average pore size Ideal for compounds >500MW. Octyl (C8) Silica 4.5% Yes 50µm 60Å Less hydrophobic than C8 Less retention of highly hydrophobic compounds. Use when C8 is too retentive. Normal Phase (Polar) Sorbent Specifications Packing Base % Carbon Endcapped Average Particle Size Pore Size Features Benefits Silica (SI) Silica 50µm 60Å Highly polar surface Most common polar phase. spe Aminopropyl (NH) Silica 5.0% No 50µm 60Å Polar phase with slight anion exchange properties Ideal for carbohydrates or generally with analyses containing hydroxyl functional groups. Cyanopropyl (CN) Silica 6.0% Yes 50µm 60Å Unique selectivity Can be used in normal phase or reversedphase modes. Diol (OH) Silica 4.0% No 50µm 60Å Polar surface with minor hydrophobic retention Wets easily and offers more reproducibility. Florisil (FL) Magnesium Silicate 75 50µm 60Å Highly polar surface Referenced in many EPA methods. Ideally suited for pesticides and metals. Florisil -PR (FL-PR) Magnesium Silicate 75 50µm 60Å Specifically tested for chlorinated pesticides Ensures most inert batches suitable for highly active compounds. Alumina Neutral (AL-N) Aluminum Oxide 30µm 00Å Alumina washed with neutral surface Interacts with highly aromatic compounds and neutral hydroxyls. Specialty Sorbent Specifications Average Particle Size Additional Information Packing Base Benefits Carbograph Graphitized Carbon 38 5µm Retains polar organics in aqueous matrices. Ideally suited for acid, baseneutral extraction of pesticides and herbicides. SCX Styrene-DVB 50µm Hydrogen counter ion retains cations; exchange capacity.0 meq/ml SAX Styrene-DVB 50µm Acetate counter ion retains anions; exchange capacity.0 meq/ml Removes/concentrates basic compounds. Removes/concentrates acidic compounds. 54

56 spe and filtration Maxi-Clean SPE Cartridges Same bed dimensions as 4mL SPE columns Process a single cartridge by syringe or multiple cartridges by vacuum Stack different cartridges for multi-step extractions Maxi-Clean Reversed-Phase Cartridges Packing Bed Weight Qty. Prevail C8 300mg mg mg Standard C8 300mg mg mg mg mg mg High-Capacity C8 300mg Large Pore C8 300mg mg mg Octyl (C8) 300mg mg mg tech tip What is a Maxi-Clean cartridge? A Maxi-Clean cartridge is an alternative format for SPE. It uses the same high-quality packing materials as the Extract-Clean columns but has a polypropylene housing with both a female luer inlet and a male luer outlet tip. This allows use of positive pressure from a syringe or negative pressure from a vacuum manifold. 0µm polyethylene frits are placed at each end of the sorbent bed. The packing material is packed and compressed to improve or optimize flow characteristics. Maxi-Clean Drying Cartridges Packing Bed Weight Qty. Sodium Sulfate 3g more info For more information about SPE, visit our website. spe Maxi-Clean Normal Phase Cartridges Packing Bed Weight Qty. Silica (SI) 300mg mg mg mg mg mg Aminopropyl (NH) 300mg mg mg Florisil (FL) 300mg mg mg mg Florisil -PR (FL-PR) 300mg mg mg mg Alumina Neutral (AL-N) 300mg mg related products Looking for vacuum manifolds? See page 6 for vacuum manifolds to process samples. technical assistance Contact Tech Support: [email protected] Online:

57 spe and filtration Extract-Clean SPE Columns General Purpose SPE Columns with a Broad Selection of Phases and Sizes Extract-Clean Reversed-phase SPE Columns Standard C8 High-Capacity C8 Prevail C8 High-Flow C8 C8 Bed Weight Column Size Qty. 50mg.5mL mg.5mL mg 4.0mL mg 4.0mL mg 8.0mL mg 8.0mL mg 8.0mL mg 5mL mg 5mL ,000mg 75mL spe Extract-Clean Normal Phase SPE Columns Silica Aminopropyl Florisil Cyanopropyl Diol Bed Weight Column Size Qty. 50mg.5mL mg.5mL mg 4.0mL mg 4.0mL mg 8.0mL mg 8.0mL mg 8.0mL mg 5mL mg 5mL ,000mg 75mL Extract-Clean Ion Exchange SPE Columns SCX SAX Bed Weight Column Size Qty. 00mg.5mL mg 4.0mL mg 4.0mL mg 8.0mL related products Looking for vacuum manifolds? See page 6 for vacuum manifolds to process samples. technical assistance Contact Tech Support: [email protected] Online: 56

58 spe and filtration Specialty SPE Columns Carbograph SPE Columns Graphitized carbon retains polar organics in aqueous matrices Acid, base-neutral extraction of pesticides and herbicides 00m /g surface area Phase Separator SPE Columns Hydrophobic membrane allows fast and simple separation of organic and aqueous mixed samples. Phase Separator Columns Column Size Qty. 4.0mL mL mL Carbograph SPE Columns Bed Weight Column Size Qty. 50mg 4.0mL mg 8.0mL mg 8.0mL mg 5mL 0 0 Filter Columns Filter columns are SPE reservoirs with two frits at the outlet end. They remove particulate matter down to 0µm from samples. Filter Columns Qty..5mL Filter Columns mL Filter Columns mL Filter Columns mL Filter Columns spe Vydac BioSelect SPE Columns For Extraction, Concentration and Clean-up of Biological Samples Vydac BioSelect SPE columns use the same media as Vydac 300Å TP reversed-phase HPLC columns, giving Vydac SPE columns similar selectivity and recovery. Applications: Desalting of polypeptide solutions Concentration of proteins and peptides Removal of HF and cleavage products from cleavage solutions Removal of lipids and strongly bound proteins Vydac BioSelect SPE Columns Column Size Pk C4, 3µm ml 50 4SPE000 3mL 50 4SPE3000 C8, 3µm ml 50 8SPE000 3mL 50 8SPE3000 Solid-Phase Extraction Example A. 5% ACN B. 30% ACN ribonuclease C. 60% ACN myoglobin 0 0 Min. Column: Vydac 4TP5405, C4, 5µm, 4.6 x 50mm Gradient: 5 to 70% ACN over 0min, with 0.% TFA A 3mL SPE column was conditioned with ml of ACN followed by ml of 5% ACN/0.% TFA. Ribonuclease and myglobin (00mg each) were then loaded in 30% ACN/0.% TFA. The column was washed with ml of 5% ACN/0.% TFA to remove weakly bound compounds, then ml of 30% ACN/0.% TFA followed by ml of 60% ACN/0.% TFA. HPLC analysis of the 5% ACN wash (A) revealed only a small amount of ribonuclease. Most of the ribonuclease eluted in the 30% ACN wash (B). The myoglobin eluted almost entirely in the 60% ACN wash (C). 57

59 spe and filtration GracePure SPE Specifications Ideal for cost-conscious labs Concise offering of popular sorbents GracePure SPE products have a concise offering of sorbents suitable for a variety of applications. Whether pharmaceutical or petrochemical, these products deliver the selectivity and high recovery you expect. Reversed-phase (Non-Polar) Sorbents Specifications % Carbon Surface Area Particle Size Sorbent Support Endcapping C8-Max Silica 7.% Yes 58m /g 50µm 60Å Polymerically bonded 7% carbon load Pore Size Feature Benefit Application Type Highest binding capacity, best for complex samples or structurally diverse analytes. C8-Aq Silica.5% Yes 58m /g 50µm 60Å Hydrophilic endcapping Water-wettable C8 ideal for aqueous samples. Phase remains active even when completely dry. C8-Low Silica 6.5% Yes 58m /g 50µm 60Å Least hydrophobic C8 phase that easily releases C8 phase very hydrophobic compounds. C8-Fast Silica 7.0% Yes 58m /g 00µm 60Å Large 00µm particle Process large volume (>500mL) or viscous samples with fast flow rates. Drugs and their metabolites in serum and plasma, pesticides Desalting proteins, pharmaceuticals, hormones, pesticides, organics in water Surfactants, oils, antibiotics Aniline, pesticides, haloethers, phthalate esters, EPA 360, 360 spe Normal Phase (Polar) Sorbents Specifications Sorbent Support % Carbon Endcapping Surface Area Particle Size Pore Size Feature Benefit Application Type Silica Silica N/A N/A 58m /g 50µm 60Å Most polar phase Able to differentiate between structurally similar compounds. Aflatoxins, pesticides, steroids, structural isomers Amino Silica 4.3% No 58m /g 50µm 60Å Dual retention Retains polar compounds, or can act as a weak anion exchanger. Easily releases strong acids when SAX binds too strongly. Florisil Magnesium Silicate N/A No 0µm 60Å Alternates polar phase Large particle size processes large sample sizes quickly. Carbohydrates, dyes, lipids, mycotoxins, strong acids Environmental Ion-Exchange Sorbents Specifications Exchange Sorbent Support Capacity Counter Ion Anion-X 8% cross-linked styrenedivinylbenzene Cation-X 8% cross-linked styrenedivinylbenzene Particle Size Feature Benefit Application Type.5meq/g Acetate form 50µm Tetramethyl ammonium functional group on polymer base material.4meq/g Hydrogen form 50µm Benzene sulfonic acid functional group on polymer base material ph range from 4, with excellent exchange capacity. ph range from 4, with excellent exchange capacity. Anionic compounds: organic acids, fatty acids Cationic compounds: amines, amino acids technical assistance Contact Tech Support: [email protected] Online: 58

60 spe and filtration GracePure SPE Columns Base Material Properties GracePure base silicas are characterized multiple ways to ensure that the starting point for every batch of media is consistent. Sorbent Characterization Tight specifications promise clean and reproducible sorbent performance. An HPLC chromatogram offers a detailed look at selectivity that is not possible with recovery tests alone. Component Tests GC/FID shows that GracePure tubes are constructed from a highly inert grade of polypropylene to prevent extractable contamination. Polyethylene frits are thoroughly washed in organic solvent, which also eliminates extractables. Manufacturing Control GracePure SPE products are packed and assembled using custom-designed, precision equipment. Every manufacturing batch is guaranteed to have less than % bed weight variation and uniform flow rates. spe GracePure Reversed-Phase SPE Columns Sorbent Bed Weight Column Volume Qty. C8-Max 50mg ml 00pk mg ml 00pk mg 3mL 50pk mg 6mL 30pk mg 6mL 30pk mg 0mL 0pk mg 0mL 0pk 5454 C8-Aq 50mg ml 00pk mg ml 00pk mg 3mL 50pk mg 6mL 30pk mg ml 30pk mg 0mL 0pk 5453 C8-Low 00mg ml 00pk mg 3mL 50pk mg 3mL 50pk mg 6mL 30pk mg 6mL 30pk mg 0mL 0pk 5456 C8-Fast 500mg 3mL 50pk mg 6mL 30pk mg 0mL 0pk 5457 GracePure Normal Phase SPE Columns Sorbent Bed Weight Column Volume Qty. Silica 00mg ml 00pk mg 3mL 50pk mg 3mL 50pk mg 6mL 30pk mg 6mL 30pk mg ml 30pk mg 0mL 0pk mg 60mL 6pk Amino 500mg 3mL 50pk mg 6mL 30pk Florisil 500mg 3mL 50pk mg 6mL 30pk mg ml 30pk 545 GracePure Ion-Exchange SPE Columns Sorbent Bed Weight Column Volume Qty. Anion-X 00mg ml 00pk mg 3mL 50pk mg 3mL 50pk Cation-X 00mg ml 00pk mg 3mL 50pk mg 3mL 50pk more info For more information about SPE, visit our website. 59

61 spe and filtration IC-SPE Cartridges Eliminate matrix interferences before ion chromatography Seven chemistries solve specific problems The most difficult part of many IC applications is eliminating interfering components from the sample matrix. These interferences may co-elute or mask peaks of interest, overload the column, or shorten the column life by binding irreversibly to the column packing. The unique ion-exchange SPE cartridge eliminates many of these interferences,. R. Saari-Nordhaus, J.M. Anderson, Jr., and I.K. Henderson, Am. Lab. August (990) 9. I.K. Henderson, R. Saari-Nordhaus, and J.M. Anderson, Jr., J. Chromatogr. 546 (99) 6. spe Ion Chromatography Sorbent Specifications Particle Packing Base Counter Ion Size IC-OH Styrene- DVB IC-H IC-Ag IC-Ba IC-Na IC-Chelate Styrene- DVB Styrene- DVB Styrene- DVB Styrene- DVB Styrene- DVB Molecular Exclusion Limit Exchange Capacity Retains Applications Hydroxide 50µm 000 Daltons.0meq/mL Anions Exchanges anions for hydroxide. May be used to remove or concentrate anions from sample and to increase ph of acidic samples. Removes cations that form insoluble hydroxide salts. Hydronium 50µm 000 Daltons.0meq/mL Cations Exchanges cations for H +. May be used to remove or concentrate cations from sample and to reduce ph of basic samples. Silver 50µm 000 Daltons.0meq/mL Chloride Iodide Bromide Removes excess halides through formation of Ag-halide salts. Barium 50µm 000 Daltons.0meq/mL Sulfate Removes excess sulfate through formation of BaSO. Sodium 50µm 000 Daltons.0meq/mL Cations Exchanges cations for Na +. May be used to remove or retain cations from sample without changing the ph of the sample. Sodium 50µm 000 Daltons 0.4meq/mL Polyvalent metal ions Exchanges transition metals and divalent cations for Na +. May be used to remove or retain divalent cations and transition metals from sample. IC-RP Polystyrene 550µm Hydrophobic components Removes surfactants, organic acids and other organic substances. Inorganic ions pass through. Unextracted IC-H Neutralizes Hydroxide Trace Anions in Caustic. Fluoride. Chloride 3. Nitrate Extracted 3 Ion Chromatography Maxi-Clean Cartridges Packing Bed Weight Qty. IC-OH 0.5mL IC-OH.5mL IC-H 0.5mL IC-H.5mL IC-Ag 0.5mL IC-Ag.5mL IC-Ba 0.5mL IC-Ba.5mL IC-Na 0.5mL IC-Na.5mL IC-Chelate 0.5mL IC-Chelate.5mL IC-RP 0.5mL IC-RP.5mL Min Min. Column: Mobile Phase: Flow Rate: Detector: Anion column, 50 x 4.6mm.7mM NaHCO3 /.8mM NaCO3.mL/min Conductivity (Suppressed) 60

62 spe and filtration Reservoirs and Frits Select empty reservoirs and loose frits to pack your own custom SPE columns. Adapters and Caps Inlet caps fit SPE devices as indicated Outlet caps fit any column or cartridge with a male luer tip Bulk Reservoirs and Frits Qty. SPE Empty Reservoirs, Polypropylene.5mL mL mL mL mL mL Polyethylene Frits for Extract-Clean Reservoirs For.5mL Reservoir For 4.0mL Reservoir For 8.0mL Reservoir For 5.0mL Reservoir 00 4 For 5.0mL Reservoir For 75.0mL Reservoir Syringe Adapters Syringe adapters fit the tops of SPE columns allowing attachment of any male luer device. This lets you process an Extract-Clean column with a syringe or add an empty reservoir to increase sample volume capacity. Syringe Adapters Qty. Syringe Adapters for.5, 4.0, and 8.0mL Columns Syringe Adapters for 75mL Columns technical assistance Contact Tech Support: [email protected] Online: Caps Qty. Inlet Caps for.5ml Inlet Caps for 4.0mL Inlet Caps for 8.0mL Inlet Caps for 5.0mL Inlet Caps for 5.0mL Inlet Caps for 75.0mL Outlet Caps for Male Luers Bulk SPE Packings The same high-quality material we use to manufacture our Extract-Clean and Maxi-Clean SPE products. Bulk SPE Packings Qty. Reversed-phase Prevail C8 Silica (%) 00g 505 C8 (6%) 00g 50 C8 (7%) 00g 503 Normal Phase Silica 00g 5 Aminopropyl 00g 56 Florisil Silica 7g 568 Florisil -PR Silica 7g 565 Ion-Exchange SCX (Cation Exchange) 00g 50 SAX (Anion Exchange) 00g 50 Carbograph SPE 0/400 Mesh 5g 769 spe 6

63 spe and filtration SPE Vacuum Manifolds - and 4-port manifolds Glass chamber for visual monitoring Accepts standard male luer SPE devices I C Vacuum Manifold Components A. Glass Chamber B. Vacuum Valve and Gauge C. Polypropylene Lid D. Stopcock Valves E. Collection Rack Plates F. Support Posts for Collection Racks G. Retaining Clips for Collection Racks H. Lid Legs I. Manifold Inlet Caps Vacuum manifolds process multiple samples simultaneously, saving time and effort. Additional accessories and replacement parts, as well as stainless steel or PTFE needles are available separately. A E F H B E I G D -Port Manifold For up to samples Also includes one waste container 4-Port Manifold Process up to 4 samples spe -Port and 4-Port Manifold and Accessories -Port Qty. 4-Port Qty. Vacuum Manifold 035 ea 04 ea Replacement Parts Lid, Gaskets, and Stopcocks 00 ea 4 ea Vacuum Gauge, Valve, and Glass 304 ea 034 ea Chamber Collection Rack 58 ea 044 ea Gaskets 074 One-Way Stopcocks Drying Attachement - /4" barb 00 ea 4 ea Waste Container 0033 Replacement Parts for All Size Manifolds Qty. Vacuum Gauge and Valve ea 03 Retaining Clips for Collections Racks 9 Female Luer Inlet 00 Female Luer Inlet 4 30 Male Luer Outlet 0 Male Luer Outlet 4 30 Caps for Lid Inlets Lid Legs, Black Collection Rack Posts /8" i.d., 7/8" o.d.* Tygon Vacuum Tubing 0' 647 *Use with Vacuum Gauge and Valve displayed above. Manifold Needles PTFE needles are disposable and eliminate cross contamination. Stainless steel and polypropylene needles are also available. Stainless Steel PTFE Manifold Needles Qty. PTFE Needles PTFE Needles Valved PTFE Needles Valved PTFE Needles Stainless Steel Needles 400 Stainless Steel Needles Polypropylene Needles 4 Polypropylene Needles Polypropylene Valved PTFE 6

64 spe and filtration Filtration Choose from a Variety of Membranes and Devices for All Your Sample Filtration Needs Nylon is hydrophilic and has good solvent resistance PVDF is hydrophilic, highly solvent resistant, and low protein binding PTFE is hydrophobic with exceptional solvent resistance Regenerated Cellulose is hydrophilic with exceptional solvent resistance and low protein binding Syringe Filters Syringe Filter Specifications Max. Temp. Max. Pressure Typical Sample Volume Diameter Hold-Up Volume 4mm 00 C 75psig Less than 5µL Up to ml 3mm 60 C 00psig Less than 0µL Up to 5mL 7mm 00 C 5psig Less than 5µL Up to 50mL 5mm 60 C 00psig Less than 50µL Up to 50mL 30mm 00 C 90psig Less than 5µL Up to 00mL Syringe Filters Nylon PVDF PTFE Regenerated Cellulose Diameter Pore Size Qty. 4mm 0.µm mm 0.45µm mm 0.µm mm 0.45µm mm 0.µm mm 0.45µm mm 0.µm mm 0.45µm mm 0.µm mm 0.45µm filtration Centrifuge Filter Tubes Centrifuge Filter Tubes Nylon PVDF Regenerated Cellulose Size Max. Sample Volume Max. G-Force Pore Size Qty. ml 850µL 0,000xG 0.µm ml 850µL 0,000xG 0.45µm mL 5mL,500xG 0.µm mL 5mL,500xG 0.45µm related products Looking for pre-cut filter membrane discs? Visit our website. more info Visit our website for a chemical compatibilty chart by filter membrane type. 63

65 general chromatography Alltech 3300 ELSD Sensitive, Universal HPLC Detection Detection of difficult compounds has never been easier. Sensitive Low noise and excellent baseline stability Low nanogram detection limits Easy to Use Step-by-step method wizard automates detector setup Easy access to parts requiring cleaning or maintenance Small Footprint Small footprint minimizes bench space requirements.6" H x 0.3" W x 9.5" D (9.5cm H x 6.cm W x 49.5cm D) Universal Detection for a Broad Range of HPLC Applications ELSD shows what may be missing from your UV chromatogram ELSD obtains a more accurate representation of sample mass than UV/Vis equipment Alltech 3300 ELSD* 3300 ELSD 0V 40V *Patent pending. Service and Support Plant Oil Extract ELSD UV at 54nm Min. Column: Alltech Alltima C8, 3µm,. x 50mm ( 4385) Mobile Phase: A: 0.% Formic Acid B: Methanol Gradient: Time: 0 40 %B: Flow Rate: 0.5mL/min Column Temp: 30 C Our dedicated technical support professionals provide you with the comprehensive support you need, including a complete line of service options. Services for the Alltech 3300 ELSD Installation and Training Qualification IQ/OQ at Customer Site IQ/OQ/PQ at Customer Site INSTALL Q33000 Q Alltech ELSDs are designed and manufactured in accordance with the following standards: EN60- + A:95, EN5550: 998, FCC CFR 47 Part 5: 00, EN 636: 998, EN : A4:000 and EN : 995. Mephobarbital (500ppm). Secobarbital (500ppm) Column: Alltech Alltima HP C8 HL, 5µm, 4.6 x 50mm, ( 87698) Mobile Phase: Acetonitrile:Water (30:70) Flow Rate:.0mL/min Column Temp: 45 C 3300 ELSD Specifications Light Source: Detector Element: Temperature Range: Nebulizer Gas: Inlet Pressure: Typical Operating Range: Flow Control: Mobile Phase Flow Rate: Analog Output: Communications: User Interface: Power Requirements: Dimensions: Weight: IEC/FDA Classification: Manufacturing Standards: Warranty: : Mixture ELSD UV at 30nm Min Laser diode with collimating optics, 650nm, 30mW output, class IIIB Silicon photodiode Ambient to 0ºC in ºC increments Nitrogen preferred; Calibrated to 4.0L/min 60 80psig.0.0L/min Digital Mass Flow Control To 3.0mL/min V or 0mV full scale Inputs: TTL/contact closure for auto zero, gas shutoff, start, and standby Outputs: Contact Closure for pump shutdown in error conditions, general events Data Ports: USB, RS3, Ethernet Full-color Microsoft Windows -based Graphical LCD with alphanumeric keypad 0/40V, 50/60Hz.6" H x 0.3" W x 9.5" D (9.5cm H x 6.cm W x 49.5cm D) 30lb (6kg) Class Laser Product; Complies with CFR Subpart J Meets all applicable safety and EMC certifications; CE, UL, and CSA certified year parts and labor 64

66 general chromatography Alltech ELSD Nitrogen Generator Simplifies ELSD operation Produces a continuous supply of high purity nitrogen from compressed air Replaces inconvenient nitrogen cylinders Requires no electricity Parker Domnick Hunter G30 High Purity Nitrogen Generator Integral oil free compressor No house air required The Alltech ELSD nitrogen generator has a small, compact footprint that fits easily on the lab bench next to your ELSD or mounts on the wall nearby. ELSD Nitrogen Generator Specifications Nitrogen Purity: 99% Nitrogen Flow: 4L/min Min./Max. Operating Pressure: 60/5psig Dimensions: 6." H x 0.7" W x 3.4" D (40.9cm H x 7.cm W x 34.0cm D) Weight: 4.5lb (9.3kg) Compressed Air Requirements: Purity: Free of water, compressor oil (0.0mg/ m 3 ), hydrocarbons, and particulates (<0.µm) Temperature: 60 F (5 C) 0 F (43 C) Compressor Outlet Pressure: 90 45psig Compressed Air Flow: 43L/min minimum High Purity Nitrogen Generator Specifications Nitrogen Purity: >99.9% Nitrogen Flow: 4L/min Min./Max. Operating Pressure: 7.5psig Dimensions: 34.4" H x 3.6" W x 6." D (87.4cm H x 34.5cm W x 66.3cm D) Weight: 85. lb (84 kg) Parker Domnick Hunter G30 High Purity Nitrogen Generator High Purity Nitrogen Generator equipment Alltech ELSD Nitrogen Generator ELSD Nitrogen Generator Maintenance Kit 6005 Accessories 50ft /4" o.d. PTFE Tubing 336 /4" Brass Nuts, 0/pk 4059 /4" Brass Ferrules, 0/pk 406 /4" /4" MPT Brass Male Connection 639 /8" /4" MPT Brass Male Connection 635 Single-Stage Pressure Regulator, 0 00psig 889 JUN-Air Air Compressor with Dryer Model JUN-Air Air Compressor with Dryer Model OF30-5BD 8939 more info For more information about gas generators, visit our website. Accessories 50ft /4" o.d. PTFE Tubing 336 /4" Brass Nuts, 0/pk 4059 /4" Brass Ferrules, 0/pk 406 /4" /4" MPT Brass Male Connection 639 /8" /4" MPT Brass Male Connection 635 Single-Stage Pressure Regulator, 0 00psig 889 more info For more information about maintenance kits, contact your Grace office. technical assistance Contact Tech Support: [email protected] Online:

67 general chromatography Hydrogen Generators for GC Applications Parker Balston Hydrogen Generator Specifications Model HPEM-00 HPEM-65 HPEM-60 HPEM-50 H-50 H-300 Flow (cc/min): Use: Fuel Gas Fuel and Carrier Max. Number of FIDs: Max. Number of Injectors/FIDs: N/A Purity: % % Water Quality: DI Water NaOH Solution Purifier Type: Dessicant Palladium Regenerative Purifier: No No Maximum Pressure (psig): Dimensions (H x W x D): 7." x 3.5" x 7.9" (43.5cm x 34.cm x 45.6cm) " x " x 3" (58cm x 30cm x 33cm) Weight: 40lb (8kg) 58lb (6kg) Parker Balston Hydrogen Generators HPEM-00, 0/0V 8690 HPEM-65, 0/0V 8690 HPEM-60, 0/0V HPEM-50, 0/0V H-50, 0V 803 H-300, 0V 80 H-300, 0V 805 equipment Parker HydroGen Mate DI Water System Economically Provide DI Water to Your Hydrogen Generator Removes organics, phosphates, chlorine, and most ionizable constituents Quick and easy installation Easy-fill dispensing nozzle No electrical requirements The Parker HydroGen Mate DI water system provides high purity deionized water to all types of Parker hydrogen generators. The system is ready to install and includes prefiltration, two DI resin exchange cartridges, dispensing nozzle and a final filter. Parker HydroGen Mate DI Water System Complete DI Water System Maintenance Kit* *Includes two replacement cartridges and one replacement final filter. more info For more information about gas generators, visit our website. 66

68 general chromatography HPLC Pumps Alltech Model 46 and 30 HPLC Pumps 3 yr warranty Both pumps are available in stainless steel or biocompatible PEEK. Low-Cost, High-Performance Isocratic Pumps Both Pumps Feature: Built-in pulse dampener and rapid refill for pulse-free operation Self-flushing pump head for longer seal-life Automatic prime/purge system for easy priming Wide flow range for broad HPLC work, including semi-preparative applications EZChrom driver and cables included The Model 46 has These Additional Features: User-settable upper and lower pressure limits Pressure monitoring on the LCD Interfacing features for convenient pump control HPLC Pump Specifications Model 30 HPLC Pump 46 HPLC Pump Flow Range: 0.0 0mL/min 0.0 0mL/min Isocratic/Gradient: Isocratic Isocratic Pressure Monitoring: No Yes Max. Operating Pressure: 6000psig (SS), 5000psig (PEEK) 6000psig (SS), 5000psig (PEEK) Flow Precision: 0.% RSD 0.% RSD Flow Accuracy: ±.0% ±.0% Dimensions: 6" H x " W x 8" D (5cm H x 6cm W x 46cm D) 6" H x " W x 8" D (5cm H x 6cm W x 46cm D) Weight: 30lb (4kg) 30lb (4kg) Warranty: 3-year parts, excluding 3-year parts, excluding seals, check valves and seals, check valves and pulse dampener; -year pulse dampener; -year labor labor Drivers: EZChrom Elite EZChrom Elite more info For more information about additional pumps, visit our website. Alltech Model 46 and 30 HPLC Pumps Model 46 HPLC Pump PEEK, 85 65VAC, 47 63Hz 4650 Stainless Steel, 85 65VAC, 47 63Hz 4650 Model 30 HPLC Pump PEEK, 85 65VAC, 47 63Hz 3050 Stainless Steel, 85 65VAC, 47 63Hz 3050 Alltech Model 46 and 30 HPLC Replacement Parts Pump Head Kit, PEEK 0800 Pump Head Kit, Stainless Steel 0799 Piston Seal Kit 0680 Piston 486 Check Valve Kit, PEEK 604 Check Valve Kit, Stainless Steel 0679 Note: All replacement parts contain parts for one pump head only. equipment technical assistance Contact Tech Support: [email protected] Online:

69 general chromatography Alltech On-Line Degassing Systems Advanced degassing technology Product range meets every need and budget Upgrade capabilities available Flow rate instability and baseline noise are the likely result of dissolved air in the mobile phase. Eliminate these problems with the Alltech on-line degassing system. Unlike helium sparging, degassing online does not require extra mobile phase preparation steps. Save time and avoid the expense, inconvenience and hazards associated with helium tanks. Degassing System HPLC Pump Detector Low-permeation Tubing Injector HPLC Column Mobile Phase Eliminate extra mobile phase preparation steps by degassing online. Choice of Two Degasser Families: Elite Degasser Family In addition to the key degassing components, the Elite family incorporates a vacuum sensor, validation output and a four-year warranty. The sensor not only detects and alerts you to leaks, it also communicates with the vacuum pump. If a change in vacuum is detected due to mobile phase flow rate changes, the pump can compensate by changing its speed. The validation output records vacuum level to satisfy ISO and system validation requirements. Select Degasser Family The Select family has identical degassing efficiency to the Elite line since it contains the same key components. However, by omitting the vacuum sensor and validation output, and applying the industry standard one-year warranty, the Select family is available at an economical price. equipment Specification Elite Degasser Family Select Degasser Family Membrane Type: PTFE AF PTFE AF Vacuum Pump Type: Continuous-run, self-adjusting speed Continuous-run, one-speed Low-permeation Fittings and Tubing: Yes Yes Vacuum Sensor: Yes No Validation Output: Yes (5mVDC/mm Hg) No Warranty: 4-year -year Flow Range Choices: Micro: Analytical: Semi-prep: 0 ml/min, 95µL internal volume 0 5mL/min, 480µL internal volume 0 0mL/min, 95µL internal volume 0 ml/min, 95µL internal volume 0 5mL/min, 480µL internal volume 0 0mL/min, 95µL internal volume Universal Power Supply: Yes Yes Dimensions: 5" H x 3" W x 0" D (3cm H x 8cm W x 5cm D) 5" H x 3" W x 0" D (3cm H x 8cm W x 5cm D) On-Line Degassing Systems Elite Family Select Family Micro, 0 ml/min, 95µL Internal Volume Channel Channel Analytical, 0 5mL/min, 480µL Internal Volume Channel Channel Channel Channel Semi-prep, 0 0mL/min, 95µL Internal Volume Channel Channel Channel Channel Channel Upgrade Kits* Micro, 0 ml Analytical, 0 5mL 5905 Semi-prep, 0 0mL 5905 *Includes degassing channel, vacuum tubing, mounting screw, identification sticker, and instructions. Face Plates* For Channel A For Channel B For 3 Channel C For 4 Channel D *To replace an existing degassing channel, only a channel upgrade kit is necessary. To add more channels, a new face plate is also required. Upgrade Labor Charge* Labor *Channel upgrade kit and face plate must be ordered separately. 68

70 general chromatography Hamilton Syringes 000 Series Gastight Syringes Gas and Liquid Tight, ml to 00mL PTFE-tipped plungers Replaceable barrels and plungers 700 Series Microliter Syringes General Purpose Syringe, 5µL to 500µL Plungers are not interchangeable or replaceable Accurate to % of the syringe volume Hamilton 000 Series Gastight Syringes Model Volume Gauge Pt. Style Repl. Needle Removable Needle Syringes (RN), " Needle Length 00.0mL mL mL mL PTFE Luer Lock Syringes (TLL), " Needle Length 00.0mL * mL * mL * mL * mL * mL * mL * 8600 *Visit our website for a complete listing of replacement needles. 700 Series Gastight Syringes Gas and Liquid Tight, 0µL to 500µL PTFE-tipped plungers Replaceable barrels and plungers Hamilton 700 Series Gastight Syringes Model Volume Gauge Pt. Style Repl. Needle Removable Needle Syringes (RN), " Needle Length 70 0µL 6s µL s µL s µL s µL s µL PTFE Luer Lock Syringes (TLL)/Without Slots 75 50µL * µL * 80 For Rheodyne, Valco VISF-, Beckman /Altex, SSI (RNR) Valves, " Needle Length µL s µL s µL µL *Visit our website for a complete listing of replacement needles. SampleLock Syringes 000 and 700 Series syringes with twist valve Hamilton SampleLock Syringes Model Volume Gauge Pt. Style Repl. Needle SampleLock Syringes, " Needle Length µL s µL s ml mL mL Hamilton 700 Series Microliter Syringes Repl. Needle Model Volume Gauge Pt. Style Qty. Cemented Needle Syringes (N), " Needle Length 75 5µL 6s ea µL 6s 3 ea µL 6s ea µL 6s 3 ea µL 6s 5 ea µL s ea µL s 3 ea µL s 5 ea µL s ea µL s 3 ea µL s 3 ea µL s ea µL s 3 ea µL ea µL 3 ea Removable Needle Syringes (RN), " Needle Length 75 5µL 6s ea µL 6s ea µL s ea µL s ea µL ea Luer Tip Syringes (LT) 70 0µL * ea µL * ea µL * ea µL * ea 8070 *Visit our website for a complete listing of replacement needles. Save Money on Model 70 with Multi-Packs Economical syringes cost less in six packs Easy identification six numbered dots provide quick syringe identification Hamilton 700 Series Six-pack Syringes (" Needle Length) Repl. Needle Model Volume Gauge Pt. Style Qty. Cemented Needle Syringes (N), " Needle Length 70N 0µL 6s RN 0µL 6s Autosampler Syringes For the Agilent 7673 and 7683 Autosamplers Standard needle length is.7" Syringes for the Agilent 7673 and 7683 Autosamplers Model Volume Gauge Pt. Style Qty. Cemented Needle Syringes (ASN) 75 5µL 3s AS µL 6s AS µL 3s-6s AS ea µL 3s-6s AS µL 3s AS ea µL 3s AS µL 3s ea µL 6s AS ea µL 3s-6s AS ea µL 3s-6s AS best value syringes 69

71 general chromatography SGE Syringes Plunger Protection Syringes General Purpose Syringes with Reinforced Plunger, 5µL and 0µL Includes certificate of conformance to NIST Autosampler Syringes syringes SGE Plunger Protection Syringes Volume Gauge Length Pt. Style Repl. Needle Qty. Fixed Needle Syringes 5µL 6 5cm ea µL 6 5cm ea µL 6 5cm µL 6 5cm Removable Needle Syringes 5µL 6 5cm 857 ea µL 6 5cm 8594 ea µL 6 5cm Micro Syringes Plunger-in-needle, 0.5µL to 5µL Liquid and gas tight to 650 atmospheres* SGE Microvolume Syringes Volume Gauge Length Pt. Style Repl. Needle/Plunger Standard Barrel 8mm o.d. 0.5µL 3 7cm µL 3 7cm µl 3 5cm µl 3 7cm µl 3 7cm µl 6 7cm µL 3 5cm 855 5µL 3 7cm 8503 Standard Barrel with Repeating Adapter µl 3 5cm µl 3 7cm µl 6 7cm µL 3 5cm 859 5µL 3 7cm 853 *Use a repeating adapter to prevent plunger blow-out at elevated pressures. Gas Tight Syringes Gas and Liquid Tight, 0µL to 0mL SGE Gas Tight Syringes, Needles included Volume Gauge Length Pt. Style Repl. Needle Removable Needle 0µL 6 5cm µL 5 5cm µL 5 5cm µL 5 5cm µL 5 5cm µL 5 5cm ml 3 5cm mL 3 5cm mL 3 5cm mL 3 5cm more info For more information about additional syringes and adapters, visit our website. SGE Autosampler Syringes Volume Gauge Pt. Style Repl. Needle Qty. For Agilent 7673 Autosamplers Fixed Needle 5µL 3 ea µL µL 6 ea µL µL 3 ea µL µL 6 ea 854 0µL For PerkinElmer Autosystem Fixed Needle 5µL 3 ea µL 6 ea µL 3 ea 8549 Gas Tight Fixed Needle 5µL 3 ea µL 6 ea 8558 Removable Needle 0.5µL 3 ea µL ea 8554 For Shimadzu Autosamplers Models AOC4 and 7 Removable Needle 0.5µL 6 ea µL ea 857 For Varian Autosamplers Model 8000 Removable Needle 0µL 5 5 ea 8576 Models 8035, 800, 800 Removable Needle 0µL ea 8580 For CTC/Fisons AS00 and AS800 Autosamplers Fixed Needle (50mm) 0µL 6 ea µL 6 ea 8540 Fixed Needle (80mm) 0µL 6 ea Gas Tight Fixed Needle (50mm) 0µL 3 ea µL 3 ea 8540 Guided Plunger Syringes Extended Barrel Syringes, 5µL and 0µL Reinforced plunger SGE Guided Plunger Syringes Volume Gauge Length Pt. Style Repl. Needle Fixed Needle Syringes 5µL 6 5cm 850 0µL 6 5cm Removable Needle Syringes 5µL 6 5cm µL 6 5cm µL 6 7cm

72 general chromatography Color-Coded PEEK Tubing Chemically inert and biocompatible High strength polymer replaces stainless steel Easy to cut with razor blade or tubing cutters PEEK tubing is the ideal metal-free tubing. Its mechanical strength, chemical resistance, and biocompatibility make it appropriate for a wide variety of high pressure LC applications. Tubing is color-coded to indicate i.d. PEEK Tubing Specifications Material: PEEK Maximum Temperature: 00 C Maximum Pressure: Varies by Size, See Order Block Typical Use: High-Pressure Plumbing Striped PEEK Tubing No dye contacts fluid path Striped PEEK Tubing Color o.d. i.d. Max. Pressure Length Striped Red /6" 0.005" 7,000psig 0' (3.05m) ' (5.5m) 3575 Yellow /6" 0.007" 7,000psig 0' (3.05m) ' (5.5m) 3573 Blue /6" 0.00" 5,000psig 0' (3.05m) ' (5.5m) Orange /6" 0.00" 5,000psig 0' (3.05m) ' (5.5m) Green /6" 0.030" 4,000psig 0' (3.05m) ' (5.5m) 357 Grey /6" 0.040" 3,000psig 0' (3.05m) ' (5.5m) Black /6" 0.055",000psig 0' (3.05m) ' (5.5m) 3574 Solid Color PEEK Tubing Entire tube is colored for faster identification Solid Color PEEK Tubing Color o.d. i.d. Max. Pressure Length Solid Red /6" 0.005" 7,000psig 0' (3.05m) ' (5.5m) 357 Yellow /6" 0.007" 7,000psig 0' (3.05m) ' (5.5m) 3573 Blue /6" 0.00" 5,000psig 0' (3.05m) ' (5.5m) 3579 Orange /6" 0.00" 5,000psig 0' (3.05m) ' (5.5m) 3577 Green /6" 0.030" 4,000psig 0' (3.05m) ' (5.5m) 3575 Natural /8" 0.06" 5,000psig 0' (3.05m) ' (5.5m) 3577 /8" 0.080" 3,000psig 0' (3.05m) ' (5.5m) 3579 Flex-Connect PEEK Tubing Self-adjusting length Will not bend or kink Chemically inert and biocompatible Includes two -piece hex-head fittings tubing Striped Flex-Connect Tubing No dye contacts fluid path Striped Flex-Connect PEEK Tubing Color o.d. i.d. Max. Pressure Size Range* Striped Blue /6" 0.00" 5,000psig 0cm cm cm cm Orange /6" 0.00" 4,000psig 0cm cm cm cm Green /6" 0.030" 4,000psig 0cm cm cm cm *Size range indicates where coil is completely retracted and pulled to a comfortable distance. Excludes 6" straight piece on each end. Solid Color Flex-Connect Tubing Entire tube is colored for fast identification of i.d. Solid Color Flex-Connect PEEK Tubing Color o.d. i.d. Max. Pressure Size Range* Solid Blue /6" 0.00" 5,000psig 0cm cm cm cm 3586 Orange /6" 0.00" 4,000psig 0cm cm cm cm Green /6" 0.030" 4,000psig 0cm cm cm cm *Size range indicates where coil is completely retracted and pulled to a comfortable distance. Excludes 6" straight piece on each end. 7

73 general chromatography Low-Pressure Tubing PTFE Tubing Maximum inertness PTFE Tubing Specifications Material: PTFE Maximum Temperature: 00 C Maximum Pressure: Varies by Size, See Order Block Typical Use: Low Pressure Plumbing PTFE Tubing o.d. i.d. Max. Pressure Length /6" 0.007",600psig 0' (3.05m) /6" 0.007",600psig 50' (5.5m) /6" 0.00",500psig 0' (3.05m) 0064 /6" 0.00",500psig 50' (5.5m) /6" 0.00",00psig 0' (3.05m) 0033 /6" 0.00",00psig 50' (5.5m) /6" 0.030" 900psig 0' (3.05m) 003 /6" 0.030" 900psig 50' (5.5m) /6" 0.040" 600psig 0' (3.05m) 006 /6" 0.040" 600psig 50' (5.5m) 33 /8" 0.063" 900psig 0' (3.05m) 0063 /8" 0.063" 900psig 50' (5.5m) 3567 /8" 0.00" 300psig 0' (3.05m) 0096 /8" 0.00" 300psig 50' (5.5m) " 0.5" 300psig 0' (3.05m) " 0.5" 300psig 50' (5.5m) /4" 0.5" 900psig 50' (5.5m) 336 Low Permeation Tubing Eliminates regassing without sacrificing inertness Flexible, convenient, and versatile Easy to install and use This low-pressure tubing has a double wall to prevent mobile phase regassing. The inert inner PTFE wall carries the mobile phase. The non-wetted outer wall is made from a translucent, flexible polymer with extremely low gas permeability. This low permeation tubing cuts regassing rates to negligible levels, while preserving PTFE's chemical resistance, visibility, and handling properties. Use with low permeation fittings kit. Low Permeation Tubing Specifications Material: PTFE, Proprietary Non-Wetted Outer Layer Maximum Temperature: 00 C Maximum Pressure: 900psig Typical Use: Low-Pressure Plumbing Low Permeation Tubing o.d. i.d. Length /8".5mm 0' 4700 /8".5mm 50' 470 Low Permeation Fittings Kit /4-8 Low Permeation Fittings Kit* 470 *Includes 0 nuts, 0 ferrules, 0 inserts. tubing tech tip Choosing a tubing material for GC analysis. A number of options are available for packed column tubing. The most inert material is glass, which should be used for active compounds. Glass-lined tubing and AT Steel provide the inert surface of glass combined with the mechanical strength of a metal column. Metal tubing provides an economical and rugged column for suitable application. Passivated nickel tubing can frequently be used with active compounds such as phenols and amines. Stainless steel tubing is recommended for hydrocarbon, fixed gas, and solvent analyses where column inertness is less of a concern. PTFE tubing is extremely inert, but due to temperature limitations and poor column efficiency, PTFE is generally only recommended for the analysis of corrosive gases which are too reactive for glass. related products See page 75 for the Clean-cut tubing cutter designed to make clean square cuts on PEEK tubing. technical assistance Contact Tech Support: [email protected] Online: 7

74 general chromatography Stainless Steel Tubing Hi-EFF Tubing Hi-EFF grade stainless steel tubing is specially tempered for easy bending and is washed with acetone to remove any residual materials. Type 36 is recommended for plumbing LC systems. Stainless Steel Tubing This stainless steel tubing is the same high quality as the Hi-EFF tubing grade, but has not been washed with acetone. Hi-EFF and Standard Stainless Steel Tubing Specifications Material: 300 Series Stainless Steel Maximum Temperature: 500 C Maximum Pressure: Varies according to i.d. Typical Use: High-Pressure Plumbing Stainless Steel Tubing 0ft (3m)* 5ft (7.5m)* 50ft (5m)* Coil 00ft (60m)* Coil o.d. i.d. Hi-EFF Type 36 Stainless Steel /8" (3.8mm) 0.085" (.6mm) /4" (6.35mm) 0.0" (5.33mm) Type 304 Stainless Steel /8" (3.8mm) 0.085" (.6mm) /4" (6.35mm) 0.0" (5.33mm) Standard Type 36 Stainless Steel /3" (0.79mm) (0.0mm) /6 (.59mm) 0.007" (0.8mm) " (0.5mm) " (0.5mm) " (0.76mm) " (.0mm) " (.7mm) Type 304 Stainless Steel /8" (3.8mm) (.6mm) /4" (6.35mm) 0.0" (5.33mm) *Meters based on closest estimate. AT Steel Activity Tested Steel Tubing Delivers the Strength of Stainless Steel and the Inertness of Deactivated Fused Silica Flexible and strong Maximum temperature limit of 340 C 350 C A combination of chemical vapor deposition techniques and silicone chemistries transforms durable stainless steel tubing into a chromatographically inert material. AT Steel activity tested steel tubing is suitable for sample loops, transfer lines and capillary and packed GC columns. AT steel tubing may be cut with standard tubing cutters or high speed wheel cutters and can be rinsed with common solvents to remove particulates and contamination that have built up during use. tubing AT Steel Tubing i.d. 6ft Length 5ft Length 50ft Length 00ft Length /6" o.d. Tubing 0.00" " " " /8" o.d. Tubing 0.085" Straight Stainless Steel Tubing Type 304 stainless steel 6ft or 0ft lengths PTFE-Coated Stainless Steel Tubing Must be preconditioned at 50 C for three hours Straight Stainless Steel Tubing o.d. i.d. Length Qty. /8" (3.8mm) 0.085" (.6mm) 6ft ST /8" (3.8mm) 0.085" (.6mm) 0ft ST /4" (6.35mm) 0.0" (5.33mm) 6ft ST /4" (6.35mm) 0.0" (5.33mm) 0ft ST PTFE-Coated Stainless Steel Tubing o.d. i.d. Length /8" (3.8mm) 0.085" (.6mm) 50ft 34 /4" (6.35mm) 0.0" (5.33mm) 50ft

75 general chromatography Stainless Steel Tubing Glass-Lined Stainless Steel Tubing (GLT) Flow lines for corrosives GC/MS interfaces Capillary column connections PTFE-Lined Stainless Steel Tubing Ideal for use with reactive chemicals and for trace quantities of water Use with PTFE ferrules Glass-Lined Stainless Steel Tubing o.d. i.d. Length /6" (.59mm) 0.5mm 80cm 364 /4" (6.35mm) 4.0mm 80cm 349 PTFE-Lined Stainless Steel Tubing o.d. i.d. Length /8" (3.8mm) 0.070" (.78mm) 6ft 35 /8" (3.8mm) 0.070" (.78mm) 0ft 354 /4" (6.35mm) 0.80" (4.57mm) 6ft 356 Metal Tubing Aluminum Tubing Economical choice for GC gas lines Polymeric Tubing Maximum inertness PFA FEP Polypropylene Aluminum Tubing o.d. i.d. Length /8" (3.8mm) 0.065" (.65mm) 50ft /6" (4.76mm) 0.8" (3.5mm) 50ft 300 /4" (6.35mm) 0.90" (4.83mm) 50ft 30 3/8" (9.53mm) 0.35" (8.00mm) 50ft 30 Copper Tubing Economical choice for GC gas lines PFA (Perfluoroalkoxy) Tubing PFA (Perfluoroalkoxy) Tubing o.d. i.d. Length /6" (.59mm) 0.030" (0.76mm) 5ft /8" (3.8mm) 0.06" (.57mm) 5ft /4" (6.35mm) 0.56" (3.96mm) 5ft FEP (Fluorinated Ethylene Polypropylene) Tubing tubing Copper Tubing o.d. i.d. Length /8" (3.8mm) 0.065" (.65mm) 50ft /6" (4.76mm) 0.8" (3.5mm) 50ft /4" (6.35mm) 0.90" (4.83mm) 50ft /8" (9.53mm) 0.35" (8.00mm) 50ft Nickel Tubing Pure nickel 00, more inert than stainless steel Acid washed and rinsed for additional inertness FEP (Fluorinated Ethylene Polypropylene) Tubing o.d. i.d. Length /6" (.59mm) 0.030" (0.76mm) 5ft /8" (3.8mm) 0.06" (.57mm) 5ft /4" (6.35mm) 0.56" (3.96mm) 5ft 4574 Polypropylene Tubing Polypropylene Tubing o.d. i.d. Length /8" (3.8mm) 0.080" (.03mm) 50ft 303 /8" (3.8mm) 0.080" (.03mm) 00ft 30 related products Need fittings? See pages for GC fittings. Nickel Tubing o.d. i.d. 50-Foot Coil /6" (.59mm) 0.040" (.0mm) 3085 /8" (3.8mm) 0.083" (.mm) 3080 technical assistance Contact Tech Support: Online: 74

76 general chromatography PEEKsil Tubing Inert fused silica bonded to PEEK Square cut and polished tubing ends for zero dead volume connections Ideal for plumbing micro, capillary and nano LC systems SGE s PEEKsil tubing maintains the easy handling and convenience of PEEK tubing, but offers the tight i.d. tolerances that can only be offered with fused silica. Each PEEKsil tubing end is cut to an exact 90 angle and polished smooth to ensure a true zero dead volume connection. Tubing is color-coded to identify i.d. It is the ideal choice for plumbing capillary and micro LC systems where conventional /6" or /3" fittings are used. PEEKsil Tubing Specifications Material: Fused Silica-Lined PEEK Maximum Temperature: 00 C Typical Use: Plumbing Capillary LC Systems Maximum Pressure: 5,000psig Tubing Cutters Rotary Tubing Cutter Cuts /3" to /8" o.d. tubing burr-free Leaves tubing i.d. open Works with metal and glass-lined tubing Plier-type Tubing Cutters Cuts /6" o.d. tubing Ideal for cutting tubing in tight places Cuts metal or plastic tubing Ceramic Fused Silica Cutter Cleanly cuts fused silica tubing Small enough to keep handy in your pocket Just score and snap PEEKsil Tubing, /6 o.d. 0cm 5/pk 5cm 5/pk Length 0cm 5/pk 50cm /pk Color i.d. (mm) Orange Natural Black Red Purple Yellow Blue Grey Flex-wrap Tubing Wrap Holds parallel tubing or wiring together Ideal for gradient pumps or purging-gas lines Chemically inert 00% PTFE Electric Tubing Cutter Cut /6", /8" and /4" o.d. tubings with internal diameters as small as 0.008" Requires no lubricant or cutting fluid Assures square and burr-free cuts Clean-cut Tubing Cutter Use with PTFE, ETFE polymer, polyolefin, PEEK Make clean, square cuts without deforming inner diameters Cuts /3" to /8" o.d. Plastic Tubing Cutter Ideal for PEEK, ETFE polymer and other plastic tubing Cuts any size up to /4" o.d. tubing Flex-wrap Tubing Wrap, 0ft /8" /" Color Blue 3593 Orange 3594 Black 3596 White 3597 related products Need high-pressure fittings? See pages 6 7. Tubing Cutters Qty. Rotary Tubing Cutter ea 365 Replacement Cutting Wheels Plier-Type Tubing Cutter ea 369 Ceramic Fused Silica Cutter Electric Tubing Cutter 0 0/0 40VAC, 50/60Hz ea Replacement Parts and Accessories Dressing Tool for /8" and /4" o.d. Tubing ea 0-08 Replacement Cutting Wheels Replacement Dressing Tool for /6" o.d. Tubing ea 0-06 Clean-Cut Tubing Cutter ea 3590 Replacement Blade ea Plastic Tubing Cutter ea 306 Replacement Blade ea

77 general chromatography LC Certified Vials We understand how a bad vial can disrupt productivity in your lab. It is for this reason that we set out to deliver a vial that can give peace of mind and help you stay focused on your results. We trace each piece of the vial, cap and septa manufacturing to ensure they are of the highest grade. The manufacturing process is constantly monitored to maintain a contaminant free environment all the way through to the kit packaging. Benefits Helps ensure peaks are from your sample, not your vial Designed to high tolerances for optimal functioning Certificate of Analysis Material specifications as set by international standards (USP, EP, ASTM, ISO) HPLC test data from batch tested assembled vials & caps Grace Davison Discovery Sciences Vial Certificate of Analysis 7500 Grace Drive Columbia, MD 044, U.S.A. U.S. Toll Free: International Tel: Fax: [email protected] Web: Part Number: : Lot Number: Grace Quality ISO cgmp compliant Glass Material The glass below is certifi ed to meet the standards of the USP XXVI, The National Formulary XXII for Type I, II, & III glass, under Physical Test-Glass Containers, as well as the ASTM E-438 specifi cation for Type I, II, & III Class A & B. Clear vials are Type 33 expansion glass, Amber vials are Type 5. Dimensional Characteristics of Vial Height I.D. Neck* O.D. at Body 3mm +/-0.5mm 6.045mm +/-0.55mm.65mm +/-0.5mm *Not applicable to fused inserts ml.ml 300µL Cap Septum Material Dimension Physical Test Chemical Test PTFE / Silicone 9mm Seal and Bond Strength Test Level II GC headspace vials LC Certified x3mm Screw Thread Autosampler Vial Kit with Blue 9mm Bonded PTFE/Silicone Cap, 00/pk Non-Slit Septa Pre-Slit Septa ml Clear with Marking Spot ml Amber with Marking Spot mL Ultra Recovery Clear* µL Limited Volume Fused Glass Insert Clear with Marking Spot *For precision extractions with less than µl dead volume. Instrument Compatibility* Agilent Gilson Beckman Hitachi Dynatech PerkinElmer *Using x3mm autosampler vials. Shimadzu Spark Thermo Varian Waters HPLC Chemical Test of Vial and Cap Assembly Tested & Conforms to Grace Method 05 Testing performed by HPLC with a Grace VisionHT C8 5um Column (Part 55993) Using the proprietary protocol of Grace Method 05 (75/5/0.- ACN/HO/TFA) QC Approval: Date: F408 0/0 GRACE and GRACE DAVISON are trademarks, registered in the United States and/or other countries, of W. R. Grace & Co.-Conn. GRACE DAVISON DISCOVERY SCIENCES is a trademark of W. R. Grace & Co.-Conn. VISIONHT is a trademark of Alltech Associates, Inc. The information presented herein is derived from our testing and experience. It is offered for your consideration and verification. Since operating conditions vary significantly, and are not under our control, we disclaim all warranties on the results that may be obtained from the use of our products. W. R. Grace & Co.-Conn. and its subsidiaries can not be held responsible for any damage or injury occurring as a result of improper installation or use of its products. Grace reserves the right to change prices and/or specifications without prior notification. technical assistance Contact Tech Support: [email protected] Online: 76

78 general chromatography Screw Thread Vials Variety of styles for virtually every autosampler Economical kits simplify ordering x3mm R.A.M. Robotic Vial Specifications 3mm mm Neck Style: Screw Thread Required Closure: 9mm R.A.M. Screw Thread Caps Wide Mouth Opening: 9mm o.d., 6mm i.d. x3mm R.A.M. Robotic Screw Thread Vials, 00/pk Vials Clear Amber Clear with White Graduated Marking Spot Amber with White Graduated Marking Spot 9553 Limited Volume Vials 00µL Polypropylene Vials µL PMP (TPX) Vials Vial Kits Clear Vials, 9mm Cap, PTFE/Silicone Septa mm R.A.M.. Open-Hole Caps Black Cap, PTFE/Silicone Septa 9534 Blue Cap, PTFE/Silicone Septa Blue Cap, PTFE/Butyl Septa Black Cap, PTFE/Silicone/PTFE Septa 9538 Septa for 9mm R.A.M.. Caps PTFE/Silicone 40mil x3mm Screw Thread Std. Mouth Specifications x3mm Screw Thread Wide Mouth Specifications 3mm mm Neck Style: Wide Mouth Screw Thread Required Closure: 0/45mm Screw Thread Caps Wide Mouth Opening: 0mm o.d., 6mm i.d. x3mm Screw Thread Wide Mouth Vials, 00/pk Vials Clear 9833 Amber 9835 Clear with White Graduated Marking Spot 989 Amber with White Graduated Marking Spot 986 Limited Volume Vials 500µL Polypropylene Vials µL Polypropylene Vials Vial Kits Clear Vials, Black 0/45 Caps, PTFE Septa 9889 Clear Vials, Black 0/45 Caps, PTFE/Silicone Septa Amber Vials, Black 0/45 Caps, PTFE Septa 9874 Amber Vials, Black 0/45 Caps, PTFE/Silicone Septa 988 Preassembled 0/45 Open-Hole Caps Black Cap, PTFE/Silicone Septa with Slit 9703 Black Cap, PTFE/Silicone Septa Black Cap, Bonded Interseal PTFE/Silicone Septa 704 Solid Polypropylene 0/45 Top Caps with Septa Black Cap, PTFE Septa 984 Septa for 0/45 Caps Red PTFE/White Silicone 5/55mil 984 PTFE/Silicone with Slit 5/55mil 980 5x45mm Screw Thread Specifications 3mm mm Neck Style: Screw Thread Required Closure: 8/45mm Screw Thread Caps Standard Mouth Opening: 8mm o.d., 4mm i.d. x3mm Screw Thread Standard Mouth Vials, 00/pk Vials Clear 959 Amber 9594 Clear with White Graduated Marking Spot Amber with White Graduated Marking Spot Limited Volume Vials.mL Glass Tapered Bottom, Requires Sleeve µL Polypropylene Vials 96 00µL PMP (TPX) Microvials µL Glass Insert in Clear Plastic Vial 980 Vial Kits Clear Vials, Black 8/45 Caps, PTFE Septa 0mil Clear Vials, Black 8/45 Caps, PTFE/Silicone Septa 45mil 9575 Amber Vials, Black 8/45 Caps, PTFE/Silicone Septa 45mil 9579 Clear Vials, Flush-Fit 8/45 Caps, PTFE/Silicone Septa Preassembled 8/45 Polypropylene Open-Hole Caps Black Flush-Fit Cap, PTFE/Silicone Septa 9806 Black Cap, PTFE/Silicone Septa 9806 Black Cap, PTFE/Silicone Septa with Slit 9708 Black Cap, Unlined Solid 8/45 Top Caps Black Polypropylene Cap, PTFE Septa Septa for 8/45 Caps Red PTFE/White Silicone 5/55mil PTFE/Silicone with Slit 5/40mil mm 5mm Neck Style: Screw Thread Required Closure: 3/45 Screw Thread Caps 5x45mm Screw Thread Vials, 00/pk Vials Clear 980 Amber 980 Clear with White Graduated Marking Spot Limited Volume Vials.5mL Polypropylene Vials µL Plastic Vial with Glass Insert 985 Vial Kits Clear Vials, Open-Hole Caps, PTFE Septa 0mil Amber Vials, Open-Hole Caps, PTFE Septa 0mil Clear Vials, Open-Hole Caps, PTFE/Silicone Septa 0/50mil Amber Vials, Open-Hole Caps, PTFE/Silicone Septa 0/50mil 9804 Preassembled 3/45 Open-Hole Caps Black Cap, PTFE/Silicone Septa 65mil 9860 Black Cap, PTFE/Silicone Septa with Slit 60mil 9707 Solid 3/45 Top Caps with Septa Solid Black Cap with PTFE/F7 Septa Solid Green Phenolic Cap with PTFE Septa 9809 Solid White Cap with Interseal PTFE/Silicone Bonded Septa 9903 Septa for 3/45 Caps Red PTFE/Silicone 5/55mil PTFE/White Silicone 0/90mil 989 vials 77

79 general chromatography Crimp Top Vials Work with most autosamplers Variety of closure styles Economical kits simplify ordering x3mm Crimp Style Std. Mouth Specifications 3mm Neck Style: Crimp Top Recommended Closure: mm Aluminum Caps Optional Closures: mm Poly Crimp Seals x3mm Crimp Style Wide Mouth Specifications Neck Style: Crimp Top 3mm Recommended Closure: mm Aluminum Caps Optional Closures: mm Poly Crimp Seals mm Standard Mouth Opening: mm o.d., 4mm i.d. mm Wide Mouth Opening: mm o.d., 6mm i.d. x3mm Crimp Style Standard Mouth Vials, 00/pk Vials Clear 6600 Amber 95 Clear, White Graduated Marking Spot Amber, White Graduated Marking Spot Limited Volume Vials 300µL Polypropylene Microvial 990 Vial Kits Clear Vials, mm Aluminum Seals, PTFE/Butyl Septa 956 Amber Vials, mm Aluminum Seals, PTFE/Silicone Septa mm Aluminum Caps* Silver Cap, PTFE/Silicone Septa 3/37mil Silver Cap, PTFE/Silicone/PTFE Septa /36/mil Silver Cap, PTFE/Red Rubber Septa 40mil Red Cap, PTFE/Red Rubber Septa 40mil 9805 Blue Cap, PTFE/Red Rubber Septa 40mil Septa for mm Aluminum Seals PTFE/Silicone 5/55mil *Caps can also be used with x3mm Crimp Style Wide Mouth Vials. x3mm Crimp Style Wide Mouth Vials, 00/pk Vials Clear 983 Amber 9845 Clear, White Graduated Marking Spot 9800 Amber, White Graduated Marking Spot Limited Volume Vials.mL Glass Tapered Bottom Vial, Requires Sleeve µL Clear Glass LVI Vial, White Marking Spot µL Amber Glass LVI Vial, White Marking Spot 9568 Vial Kits Clear Vials, mm Aluminum Seals, PTFE/Silicone Septa 9849 Amber Vials, mm Aluminum Seals, PTFE/Silicone Septa mm Poly Crimp Seals* Clear Seal, PTFE/Silicone Septa with Starburst Clear Seal, PTFE/Silicone Septa 9534 Red Seal, PTFE/Silicone Septa 9593 Clear Seal, PTFE Septa 0mil 9530 Blue Seal, PTFE Septa 0mil 9535 *Seals can also be used with x3mm Crimp Style Standard Mouth Vials. vials x3mm Crimp Style Wide Mouth Specifications 3mm mm Neck Style: Snap Ring Recommended Closure: mm Snap Top Caps Optional Closures: mm Aluminum Caps mm Poly Crimp Seals Wide Mouth Opening: mm o.d., 6mm i.d. x3mm Snap Ring Style Wide Mouth Vials, 00/pk Vials Clear Amber Limited Volume Vials 300µL Glass Insert in Clear Glass Vial µL Polypropylene Vial µL TPX (PMP) Vial µL Polypropylene Vial µL Polypropylene Vial µL TPX (PMP) Microvial 968 Vial Kits Clear Vials, mm Snap Top Caps, PTFE/Silicone Septa with Split mm Snap Top Caps Clear Cap, Polyethylene Septa 0mil 9870 Clear Cap, PTFE/Silicone Septa 0mil Clear Cap, PTFE/Silicone Septa with Slit 9868 Clear Cap, PTFE/Silicone Septa with Starburst 985 Clear Cap, PTFE/Red Rubber Septa 40mil Clear Cap, PTFE Septa 0mil x45mm Crimp Style Vial Specifications 45mm 5mm Neck Style: Snap Seal 5x45mm Snap Seal Crimp Style Vials, 00/pk Vials Clear Amber mm Aluminum Seals Silver Seal, PTFE/Silicone Septa 9883 Silver Seal, PTFE/Silicone/PTFE Septa Silver Seal, White PTFE/Butyl Septa Gold Seal, Pharma-Fix Septa mm Septa for Aluminum Seals PTFE/Silicone 0/90mil more info Looking for a hand crimper? Visit our website for crimpers, decappers and other accessories. Recommended Closure: 3mm Aluminum Seals 78

80 general chromatography Headspace Vials Crimp top and screw thread styles available Choice of vial sizes from 5mL to 7mL 0mm Seals and Septa for Headspace Vials 0mm crimp top seals with assembled liners Magnetic seals to allow automatic pick up Polyethylene snap caps for use without crimping tools 0mm Polyethylene Seal Types of Aluminum Seals Flat Top, Flat Bottom, 0mL Flat Top, Round Bottom, 0mL Bevel Top, Flat Bottom, 0mL Center Hole Magnetic Caps Pressure Release (PR) Center Tear-Off Tear-Away 5mm Center Hole 8mm Center Hole Bevel Top, Round Bottom, 0mL Screw Top, Flat Bottom, 0mL Headspace Vials 00/pk Flat Top, Flat Bottom, Clear Glass, Long Neck.5x75.5mm, 0mL Vials x46mm, 0mL Vials 6655 Flat Top, Round Bottom, Clear Glass.5x75.5mm, 0mL Vials for CTC PAL, Long Neck x75.5mm, 0mL Vials for SPME** Bevel Top, Flat Bottom, Clear Glass 3x75mm, 0mL Vials x46mm, 0mL Vials 9879 x38mm, 6mL Vials 993 Bevel Top, Round Bottom, Clear Glass 3x75.5mm, 0mL Vials x38.5mm, 5mL Vials Shimadzu Headspace Vials 30x60mm, 7mL, Clear Vials Tekmar Headspace Vials 8x50mm, 9mL, Clear Vials, 5/pk* 98098* 8x65mm, ml, Clear Vials, 5/pk* 9804* Screw Thread Headspace Vials for CTC PAL*** 0mL Clear mL Amber mL Clear mL Amber *Correct quantity specified in description. **SPME Vial has thicker glass top so cap with thinner septum will crimp tightly. ***New units only. Older models have weak magnets. 0mm Seals and Septa for Headspace Vials Qty. Polyethylene Seal with PTFE/Silicone Septum 5/45mil Aluminum Seals, Open Center Hole with Septa Seal, PTFE/Silicone Septum 0/50mil Seal, PTFE/Silicone Septum 5mil Seal, PTFE/Chlorobutyl Septum Seal, Aluminum/Silicone Septum Seal, Black Butyl Rubber Septum Specialty Seals with Septa Seal with Pharma-Fix Septum PR Seal, PTFE/Silicone Septum 8mil PR Seal, PTFE/Gray Butyl Septum 8mil Magnetic, 5mm Center, PTFE/Silicone Septum 8mil Magnetic, 8mm Center, PTFE/Silicone Septum 8mil Magnetic, 5mm Center, PTFE/Gray Butyl Septum 8mil Magnetic Seal for SPME Vial, PTFE/Silicone Septum.5mm Aluminum Seals without Septa Standard Seals, 0mm Center Hole Tear-Away Seals, Silver Tear-Off Seals, Gold Tear-Off Seals, Green Tear-Off Seals, Red Tear-Off Seals, Blue Magnetic Tinplate Seal, 5mm Center Septa for 0mm Aluminum Seals Red Silicone Septum PTFE/Silicone Septum 0/90mil PTFE/Gray Butyl Septum Stoppers for 0mm Crimp Top Vials Natural Rubber Stoppers Butyl Rubber Stoppers Magnetic Screw Thread Caps with Septa for Screw Thread Headspace Vials White PTFE/Blue Silicone Septum,.3mm PTFE/Red Butyl Septum,.6mm Blue PTFE/White Silicone Septum,.5mm vials 79

81 product index product index -Port Vacuum Manifold 6 4-Port Vacuum Manifold ELSD 64 A Adapters, HPLC 8 Adapters, Flash System 35 Adapters, Syringe 6 Adapters and Caps, SPE 6 Adsorbosil TLC Plates 36 Air Purifiers 50 All-Guard Cartridges, HPLC Columns 3,, 3, 5, 3 All-Guard Cartridges, IC Columns 5 ALL-Pure Gas Purifiers 50 Alltech 3300 ELSD 64 Alltima HP Columns 6, 3 ALTEF Gas Sampling Bags 5 Analtech TLC Plates 36 AT Steel Tubing 73 Autosampler Syringes B Big Traps Bulk SPE Packings 6 C Cali-5-Bond Gas Sampling Bags 5 Capillary HPLC Columns and Guards Alltima HP 3 VisionHT Vydac Everest 0 Vydac MS 9 Carbograph SPE Columns 57 Carbograph GC Packings 45 Carbohydrate ES, Prevail HPLC 5 Carbon Dioxide (CO) Traps 50 Carbosphere GC Packings Centrifuge Filter Tubes 63 Chromosorb GC Supports 4 45 Columns, GC Columns, HPLC 5 Crimp Top Vials 78 CTR GC Packed Columns 4 Custom Capillary GC Columns 4 Custom Packed GC Columns 4 Custom GC Coated Packings 43 D Deactivated Injection Liners 5 Deactivated SGE FocusLiners 5 Degassers 68 Direct-Connect Filter Kit 7 E Easy-Flange Tool 8 Econo-Cap GC Columns 38, 4 Elite Degasser 68 ELSD, Alltech ELSD Nitrogen Generator 65 Empty HPLC Hardware 7 Encased Metal-Free Frits 7 Everest HPLC Columns 3, 5 6, 0 Expedite, HPLC Columns Alltima HP 3 Prevail 5 VisionHT Vydac ProZap Extract-Clean SPE Columns.. 56 F FEP Gas Sampling Bags 5 Ferrules, GC 47 Filters, Syringe 63 Filtration 63 Fittings, GC Fittings, HPLC 6 7 Flange-Free Low-Pressure Fittings.. 7 Flange Tubing Low-Pressure Fittings 7 Flash Accessories 35 Flash Cartridge Kits 35 Flash Cartridges 3 34 Flash Chromatography 3 Flash System Adapters 35 Flash TLC Plates 35 Flex-Connect PEEK Tubing 7 Flex-wrap Tubing Wrap 75 Frits, HPLC 7 G Gas Purification Cartridge Systems 50 Gas Purifiers 50 Gas Sampling Bags 5 GC Capillary Columns 38 4 GC Ferrules 47 GC Packed Columns 4 GC Packings and Supports GC Septa 46 GracePure SPE Columns GraceResolv Flash Cartridges 34 GraceSmart HPLC Columns 6, 6 7 Guard Columns, HPLC Columns Alltima HP 3 Prevail 5 VisionHT Vydac Everest 0 Vydac MS 9 Vydac TP 3 Guard Columns, IC Columns 5 H Hamilton Syringes 69 Hastelloy C Slip-On Inlet Filter 30 HayeSep Porous Polymer 45 Headspace Vials 79 Heliflex Capillary Columns Helium Purifier 50 High-Pressure Adapters, Plugs, Caps 8 High-Pressure Fittings, HPLC 6 High Purity Nitrogen Generator 65 HPLC Columns Application-Based Column Selection 6 Alltima HP 3 Empty HPLC Hardware 7 GraceSmart 6 7 Hardware Formats 3 Phase Specifications and Usage 4 5 Prevail 4 5 VisionHT 8 Vydac Everest 0 Vydac MS 8 9 Vydac ProZap Vydac TP 3 HPLC Pumps 67 Hydrocarbon Traps 50 Hydrogen Generators 66 Hydrogen Purifier 50 I IC Columns 3, 4 5 IC-SPE Cartridges 60 Inlet Filters, HPLC 30 In-Line Filter Kit 7 In-Line Style Traps 49 J JUN-Air Air Compressor 65 L LC Certified Vials 76 Low-Pressure Adapters and Plugs 8 Low-Pressure Fittings, HPLC 7 Low-Pressure Tubing 7 M Macherey-Nagel TLC Plates 36 Maxi-Clean SPE Cartridges 55 Metal-Free Inlet Filter 30 Method Development Ultra MD Kits.. Microbore, HPLC Columns Alltima HP 3 GraceSmart 7 Prevail 5 VisionHT Vydac MS 9 Vydac Everest 0 Vydac TP 3 Mobile Phase Management 30 Moisture Traps Molecular Sieves 44 N Nitrogen Generators 65 Nitrogen Purifier 50 O One-Piece Couplers 8 One-Piece High-Pressure Fittings 6 On-Line Degassing Systems 68 Oxygen Traps P Packed Columns, GC 4 Packings and Supports, GC Parker Fittings 48 Parker HydroGen Mate 66 PEEK Tubing. Color-Coded 7 PEEKsil Tubing 75 Phase Separator SPE Columns 57 Plugs, HPLC 8 Polymeric Tubing 74 Porapak Packings 45 Porous Polymers 45 Prefilters, HPLC 7 Preparative, HPLC Columns VisionHT Vydac MS 9 Vydac TP 3 Pretested GC Packings 43 Prevail HPLC Columns 6, 4 5 Prevail Carbohydrate ES Columns 5 Preval Sprayers 37 ProZap HPLC Columns 3, 5 6, PTFE Ferrules 47 Pumps, HPLC 67 Pump Replacement Parts 67 R Reservoirs and Frits 6 RevealX Detection Technology 3 Reveleris C8 HPLC Columns 35 Reveleris C8 Scouting Columns 35 Reveleris Flash Cartridges 33 Reveleris Flash Chromatography System Verification Kits 35 Reveleris Flash Systems 3 Reveleris Method Development TLC Plates 35 Rheodyne Column In-Line Filter Kit 7 Rheodyne Analytical Injectors 9 Rheodyne Manual Switching Valves 9 Rheodyne Replacement Parts 9 Rheodyne RheBuild Kits 9 Rheodyne Sample Loops 9 Rheodyne Switching Valves 9 Rocket, HPLC Columns Alltima HP 3 Prevail 5 VisionHT S Sample Loops, HPLC 9 Sample Applicators 37 Screw Thread Vials 77 Seals and Septa for Headspace Vials 79 Select Degasser 68 Septa, GC 46 SGE Syringes 70 Solid Loaders 35 Solvent Filtration Apparatus 30 Solvent Reducer, HPLC Columns Alltima HP 3 Prevail 5 VisionHT Vydac MS 9 Vydac TP 3 SPE 53 6 SPE Cartridges, IC 60 SPE Cartridges, Maxi-Clean 55 Specialty SPE Columns 57 SPE Columns, Extract-Clean 56 SPE Columns, GracePure SPE Sorbent Specifications 54 SPE Vacuum Manifolds 6 Spraying Accessories 37 Sulfur Traps 50 Swagelok Tube Fittings 48 Switching Valves, Rheodyne 9 Syringe Adapters 6 Syringe Filters 63 Syringes T Tenax Polymers 45 TLC Accessories 37 TLC Plates 36 TLC Plates, Flash 35 Tubing 7 75 Tubing Accessories 75 U UHPLC Columns, VisionHT UHPLC Guard, VisionHT Ultra MD Kits Universal Mini Filters, HPLC 7 V Vacuum Filtration System 30 Vacuum Manifolds, SPE 6 Valco High-Pressure Fittings 6 Valves and Sample Loops, HPLC 9 Vespel Ferrules 47 Vespel /Graphite Ferrules 47 Vials VisionHT 6, 8 VisionHT Guard Columns 3, Vydac BioSelect SPE Columns 57 Vydac Everest HPLC Columns 3, 5 6, 0 Vydac MS HPLC Columns 3, 5 6, 8 9 Vydac ProZap HPLC Columns 3, 5 6, Vydac TP HPLC Columns 3, 5 6, 3 80

82 W. R. Grace Grace is a leading global supplier of catalysts, packaging materials and specialty construction materials. The company s three industry-leading business segments are Grace Catalysts Technologies, Grace Materials Technologies and Grace Construction Products. To learn more about how Grace products enrich lives everywhere, visit our website Global Presence, Local Expertise In 9, Grace was the first company to commercialize silica gel. Through investment in research and strategic acquisitions, Grace continuously expands our product range and global reach. With key manufacturing sites in North and South America, Europe, and Asia, plus an extensive international sales and distribution network, separation scientists throughout the world can count on timely delivery and expert local technical service. Grace understands our customers needs no matter where they do business -- we ve made the language of separations science universal. Commitment to Quality and Safety Grace embraced Six Sigma methodologies in 996. A LEAN Six Sigma corporate culture provides the foundation for continuous improvement in processes and products. Grace s commitment to quality is demonstrated by our REACH, ISO 900, ISO 3485, Pharmaceutical Excipient GMP certifications, and SOCMA awards for EH&S management. Chromatography Essentials In this catalog, you will find the best of our analytical-scale HPLC and GC products, including columns and popular accessories. We also feature our extensive SPE offering and new high performance Flash cartridges. To see the full breadth of our product offering, including pharmaceutical intermediates, large-scale purification technologies and formulation excipients, visit our website.

83 GRACE, GRACE DAVISON and GENESIS are trademarks, registered in the United States and/or other countries, of W. R. Grace & Co.-Conn. ADSORBOSIL, ALLTECH, DEACTIGLAS, DENALI, EVEREST, HELIFLEX, REVELERIS and VYDAC are trademarks, registered in the United States and/or other countries, of Alltech Associates, Inc. ADSORBOSPHERE, ALLSEP, ALLSPHERE, ALLTIMA, ALL-GUARD, ALL-PURE, APEX, APOLLO, AT, BRAVA, CARBOSPHERE, CLEAN-CUT, DIRECT-CONNECT, EASY-FLANGE, ECONO-CAP, ECONOSPHERE, ELITE, EXPEDITE, EXTRACT-CLEAN, FINGER- TIGHT, FLANGE-FREE, FLEX-CONNECT, GAS CHROM, GRACEPURE, GRACERESOLV, GRACESMART, GRAPH- TITE, GROM SIL, GROM SAPPHIRE, HI-EFF, HYDRO-PURGE, MACROSPHERE, MAXI-CLEAN, NAVIGATOR, NO- SLIP, NOVOSEP, OXI-CLEAR, OXY-PURGE, OXY-TRAP, PLATINUM, PREVAIL, PROSPHERE, PRO-ZAP, ROCKET, SELECT and VISIONHT are trademarks of Alltech Associates, Inc. ACE is a trademark, registered in the United States and/or other countries, of Advanced Chromatography Technologies AN is a trademark of Transgenomic Inc. ASCENTIS is a trademark, registered in the United States and/or other countries, of Sigma-Aldrich Biotechnology LP. BALSTON and HYDROGEN MATE are trademarks, registered in the United States and/or other countries of, Parker Intangibles LLC. CALI-5-BOND is a trademark, registered in the United States and/or other countries, of Calibrated Instruments, Inc. Carbowax is a trademark, registered in the United States and/or other countries, of Union Carbide Corporation. CARBOGRAPH is a trademark of Lara Laboratori Analitici Di Ricerca Associati Srl Chromosorb is a trademark, registered in the United States and/or other countries, of Celite Corporation. Chemipack is a trademark, registered in the United States and/or other countries, of Getinge USA, Inc. PARKER and DOMNICK HUNTER are trademarks, registered in the United States and/or other countries, of Parker Hannifin Limited. DRUMMOND and WIRETROL are trademarks, registered in the United States and/or other countries, of Drummond Scientific Company. FLORISIL is a trademark, registered in the United States and/or other countries, of U.S. Silica Company. GASTIGHT and HAMILTON are trademarks, registered in the United States and/or other countries of, Hamilton Company. MICROLITER and SampleLock are trademarks of HAMILTON GEMINI, KINETEX, and LUNA are trademarks, registered in the United States and/or other countries of, Phenomenex, Inc. HASTELLOY is a trademark, registered in the United States and/or other countries, of Haynes International, Inc. Hayesep is a trademark, registered in the United States and/or other countries, of Hayes Separation, Inc. HYPERSIL is a trademark, registered in the United States and/or other countries, of Thermo Hypersil-Keystone, Inc. INERTSIL is a trademark, registered in the United States and/or other countries, of GL Sciences, Inc. KEL-F is a trademark, registered in the United States and/or other countries, of 3MCompany. KROMASIL is a trademark, registered in the United States and/or other countries, of EKA Chemicals. JUN-AIR is a trademark, registered in the United States and/or other countries, of Gast Manufacturing Inc. and GastGroup, Ltd. MICRO-SEP is a trademark, registered in the United States and/or other countries, of Saint-Gobain Performance Plastics Corporation. MICROSOFT WINDOWS is a trademark, registered in the United States and/or other countries, of Microsoft Corporation. OXI CLEAR is a trademark, registered in the United States and/or other countries, of LabClear. PAT, PHEODYNE, RHEBUILD are trademarks, registered in the United States and/or other countries, of Rheodyne, LLC. PEEKSIL is a trademark of SGE International Pty Ltd. PERKINELMER is a trademark, registered in the United States and/or other countries, of PerkinElmer, Inc. PREVAL SPRAYER is a trademark, registered in the United States and/or other countries, of CA Acquisition, LLC DBA/AKA/TA: DBA Chicago Aerosol.R.A.M. is a trademark of J.G. Finneran Associates, Inc. ROBITUSSIN is a trademark, registered in the United States and/or other countries, of Wyeth LLC. SGE is a trademark, registered in the United States and/or other countries, of SGE Analytical Science Pty. Ltd. SWAGELOK is a trademark, registered in the United States and/or other countries, of Swagelok Company. SHIMADZU is a trademark, registered in the United States and/or other countries, of Shimadzu Corporation. TEDLAR and VESPEL are trademarks, registered in the United States and/or other countries, of E.I. Du Pont De Nemours and Company. TENAX is a trademark, registered in the United States and/or other countries, Buchem B.V. THERMOQUEST is a trademark of ThermoQuest Corporation. TYGON is a trademark, registered in the United States and/or other countries, of Saint-Gobain Performance Plastics Corp. ULTRA MD is a trademark, registered in the United States and/or other countries of, Ultraceuticals Pty. Ltd. Unibeads is a trademark of Mitsubishi Chemical Company Uniplates is a trademark of Analtech, Inc. VALCO is a trademark, registered in the United States and/or other countries, of Valco Instrments Company, Inc. ACQUITY, ATLANTIS, WATERS and XTERRA is a trademark, registered in the United States and/or other countries, of Waters Investment Ltd. PORAPAK is a trademark of Waters Investment Ltd. X-BRIDGE is a trademark, registered in the United States and/or other countries, of Software Aktiengesellschaft This catalog is an independent publication and is not affiliated with, nor has it been authorized, sponsored, or otherwise approved by Agilent, Beckman, Dionex Corporation, Dynatech, Gilson, Hitachi, Spark, and Varian or any of the companies referenced above. This trademark list has been compiled using available published information as of the publication date of this brochure and may not accurately reflect current trademark ownership or status. Copyright 0 Alltech Associates, Inc. All rights reserved.

84 Global Headquarters W. R. Grace & Co.-Conn 7500 Grace Drive Columbia, Maryland 044 USA North America 05 Waukegan Road Deerfield Illinois Tel: Fax: [email protected] The Netherlands PB 6596 NL-480 HN Breda Netherlands Tel: +3 (0) Fax: +3 (0) [email protected] Russian Federation 8, Yamskogo Polya 3rd Street Moscow, 5040 Russia Tel: [email protected] China 9th Floor, K. Wah Center 00 Huai Hai Zhong Road Shanghai 0003 China Tel: Fax: France 33 Route de Gallardon BP Epernon France Tel: +33 (0) Fax: +33 (0) Mail: [email protected] Australia Kerr Court Rowville, Vic 378 Australia Tel: Fax: [email protected] Hong Kong Units 00-3, 0 / F AXA Centre 5 Gloucester Road Wanchai Hong Kong Tel: Fax: Japan Kohken New River Bldg 3F --8, Shinkawa Chuo-ku, Tokyo Japan Tel: Fax: Germany In der Hollerhecke Worms Germany Tel: +49( 0) 64/ Fax: + 49 (0) 64/ [email protected] Italy Via Trento Passirana di Rho MI Italy Tel: Fax: [email protected] New Zealand PO Box 0035 North Shore Mail Centre North Shore City, 0745 New Zealand Tel: Fax: [email protected] India 7, Commerce Centre, Paud Road, Kotharud Pune India Tel: [email protected] Belgium Brandstraat B-960 Lokeren Belgium Tel: +3 (0) Fax: +3 (0) [email protected] United Kingdom Unit 6, Boundary Way Kellet Road Industrial Estate Carnforth, LA5 9XP, UK Tel: +44 (0) Fax: +44 (0) [email protected]