TCI Chiral HPLC Column
|
|
- Roberta Eaton
- 8 years ago
- Views:
Transcription
1 TCI Chiral HPLC Column ~ Spiral Polymer New Chiral Stationary Phase ~ Tokyo Chemical Industry Co.,Ltd. Chromatography Department 29 Tokyo Chemical Industry Co., Ltd.
2 Features of TCI Chiral A unique new stationary phase Polymaleimide spiral polymer and chiral side group coated on Silicagel. Suitable for separating of a wide range compounds Carbonyls (Ketones, Esters, Carboxylic acids, N-Protected Amino acid), Alcohols and others. Performance for both normal phase and reversed phase. Superior column longevity Maximum pressure of 29psi (2MPa) High throughput analysis High column load capacity Affordable price 29 Tokyo Chemical Industry Co., Ltd.
3 Mechanism of Chiral Recognition 1. Steric recognition of the spiral polymer area 2. Steric recognition of the side group (substituent area) 3. Electrostatic interaction between the analyte and the side group 4. PI-PI and other interactions between the analyte and the side group 29 Tokyo Chemical Industry Co., Ltd.
4 Suitable Analytes Molecule size pka Typically better separation is obtained for smaller size compounds than for larger size compounds. Ex. MW~4 is better than ~8 Neutral and acidic compounds are more often successfully separated than basic compounds. Recognition, target functional groups 1: Esters, Lactones, Ketones 2: N-protected amino acids, Carboxylic acids 3: Alcohols, Diols, Phenols 4: thers 29 Tokyo Chemical Industry Co., Ltd.
5 Analytical Condition Normal Phase (Standard starting condition) n-hexane / IPA = 9/1 Elution time is long increase IPA ratio (ex.3%) Elution time is short decrease IPA ratio (ex.1%) Acidic analyte add.1% of Trifluoroacetic acid Basic analyte add.1% of Diethylamine Reversed Phase (Standard starting condition) Acetonitrile / Water = 2/8 Elution time is long increase acetonitrile ratio (ex.4%) Elution time is short decrease acetonitrile ratio (ex.1%) Acidic analyte add.1% of Trifluoroacetic acid Basic analyte choice buffer solution Non-usable solvents THF, Toluene, Halogenated solvents (Chloroform, etc.) 29 Tokyo Chemical Industry Co., Ltd.
6 N TCI Chiral Product Line TCI Chiral MB-S TCI Chiral BP-S TCI Chiral CH-S Column N N N N N Particle size 3μm, μm 3μm, μm 3μm, μm Retention Power (Normal Phase) Analyte High Medium Weak Carbonyl (Ketones, Esters, Carbonyl (Ketones, Esters, Carbonyl (Ketones, Esters, Lactones, Carboxylic Lactones, Carboxylic Lactones, Carboxylic acids, acids, Amides, Lactams, acids, Amides, Lactams), Amides, Lactams, Amino Amino acid derivatives), mono-alcohols, acid derivatives), mono-alcohols, Diols, Diols, Ethers, Epoxides, Diols, Ethers, Epoxides, Sulfoxides Heterocycles Heterocycles, Sulfoxides 29 Tokyo Chemical Industry Co., Ltd.
7 Stability Test Column : uv TCI Chiral BP-S Column Size : 4.6mmID mm 2 Mobile Phase : Hexane/IPA = 9/1 Detection : UV 4 nm 2 Flow Rate :.7 ml/min Temperature : 4 Sample : Benzoin 17 injection α Rs Injection:1 1 Injection:4 1 Injection:6 7 Injection:8 Injection: min 29 Tokyo Chemical Industry Co., Ltd.
8 Batch to Batch Reproducibility alpha Benzoin 1-Phenyl-1,2- ethandiol. 1st 2nd 3rd 4th th 6th 7th TCI Chiral MB-S (3μm) 29 Tokyo Chemical Industry Co., Ltd.
9 High Throughput Screening Column : TCI Chiral MB-S (3μm) Mobile Phase : Hexane/IPA = 9/1 Detection : UV 4 nm Temperature : 4 Sample : Benzyl DL-Mandelate It is possible to do one minute screening Using a short column and high flow rate. mv mmID mm Flow Rate: 1.mL/min mmID mm Flow Rate:.mL/min min Tokyo Chemical Industry Co., Ltd.
10 Column Load Capacity Test Analysis min Column Size : 4.6mmI.D. mm Flow Rate : 1. ml/min Sample Load:.mg Time(min) Load Capacity Test Analysis column Column Size : 4.6mmI.D. mm Flow Rate : 1. ml/min N-Cbz-proline Column Mobile Phase Temperature : 4ºC Detection : UV4nm Load Capacity Test Prep column N H : TCI Chiral MB-S (µm) : n-hexane/2-propanol/tfa =9/1/.1 Column Size : 2.mmI.D. mm Flow Rate : 9. ml/min 1 1 Sample Load: 2mg Sample Load: 4mg min Time(min) Time(min) 29 Tokyo Chemical Industry Co., Ltd.
11 Normal and Reversed Phase Application Column Size : 4.6mmID mm Detection : UV21nm Flow Rate : 1.mL/min Temperature : 4 H Butyl Lactate mv Normal Phase : Hexane/IPA=7/3(MB-S), 8 9/1(BP-S and CH-S) MB-S BP-S CH-S min min min Reversed Phase : Acetonitrile/Water=1/ MB-S BP-S CH-S min min min 29 Tokyo Chemical Industry Co., Ltd.
12 Normal and Reversed Phase Application Column Size : 4.6mmID mm Detection : UV21nm Flow Rate : 1.mL/min Temperature : 4 Normal Phase : Hexane/IPA=9/1 6 Glycidyl Butyrate MB-S 2 1 BP-S CH-S min min min Reversed Phase : Acetonitrile/Water=1/ MB-S 2 1 BP-S CH-S min min min 29 Tokyo Chemical Industry Co., Ltd.
13 N-Protected Alanine Application N-Acetyl N-BC N-CBZ N-FMC Alpha Rs N-Acetyl alanine Column : TCI Chiral BP-S (μm) Column Size : 4.6mmID mm Mobile Phase : Hexane/IPA/TFA=9//.1 Detection : UV21nm (N-Acetyl, N-BC) UV4nm (N-CBZ, N-FMC) Flow Rate : 1.mL/min Temperature : 4 N-BC alanine min min 1 1 N-CBZ alanine N-FMC alanine min min 29 Tokyo Chemical Industry Co., Ltd.
14 TCI Chiral and Natural Polymer Application Comparison Carvone 1,4-Di--benzylthreitol H H CH 2 CH 2 Ph H H CH 2 CH 7. 2 Ph min TCI Chiral MB-S min 7 6 Amylose type min TCI Chiral MB-S min Amylose type min TCI Chiral BP-S Amylose type min min TCI Chiral BP-S min Amylose type TCI Chiral CH-S min Cellulose type min min TCI Chiral CH-S min Cellulose type 29 Tokyo Chemical Industry Co., Ltd.
15 min min min TCI Chiral and Natural Polymer Application Comparison Guaiacol Glycerol Ether H H min min min min min Mevalonolactone min min TCI Chiral MB-S Amylose type 1 TCI Chiral MB-S Amylose type 1 H min TCI Chiral BP-S Amylose type 2 TCI Chiral BP-S Amylose type min TCI Chiral CH-S Cellulose type TCI Chiral CH-S Cellulose type 29 Tokyo Chemical Industry Co., Ltd.
16 TCI Chiral and Natural Polymer Application Comparison Butyl Lactate H Glycidyl p-toluenesulfonate S min min min min min min TCI Chiral MB-S Amylose type 1 TCI Chiral MB-S Amylose type min min min min min TCI Chiral BP-S Amylose type 2 TCI Chiral BP-S Amylose type min TCI Chiral CH-S Cellulose type TCI Chiral CH-S Cellulose type 29 Tokyo Chemical Industry Co., Ltd.
17 TCI Chiral and Natural Polymer Application Comparison Chloropropionic Acid Cl min TCI Chiral MB-S 7 H min 9 Amylose type Isopropyl-3-propionyl-2- oxazolidinone min TCI Chiral MB-S N min Amylose type min TCI Chiral BP-S min 1 Amylose type min TCI Chiral BP-S min Amylose type min TCI Chiral CH-S min Cellulose type min TCI Chiral CH-S min Cellulose type 29 Tokyo Chemical Industry Co., Ltd.
18 SFC Application CH C CH 2 H Benzyl DL-Mandelate Time(min.) Column : TCI Chiral MB-S (3µm) Column Size : 4.6mmID 1mm Temperature : 4ºC Pressure : 2MPa Mobile Phase : C 2 /IPA=9/1 Detection : UV4nm Flow Rate : 2.mL/min Sample : Benzyl DL-Mandelate.99mg/mL(IPA) Injection Vol. : µl Column Pressure: 2.4MPa SFC : Supercritical Fluid Chromatography 29 Tokyo Chemical Industry Co., Ltd.
19 Complete TCI Chiral Product Line Column Type TCI Chiral MB-S TCI Chiral BP-S TCI Chiral CH-S Particle Size 3μm μm 3μm μm 3μm μm 4.6mm mm S381 S384 S38 S38 S383 S mm 1mm S381 S384 S382 S38 S383 S mm mm S3811 S3841 S3821 S381 S3831 S3861 Cartridge type guard for 4.6mm S3849(1/Pack) S389(1/Pack) S3869(1/Pack) column (3.mm 1mm) 2.mm mm S3816 S3846 S3826 S386 S3836 S mm 1mm S3812 S3842 S3822 S382 S3832 S mm mm S3813 S3843 S3823 S383 S3833 S3663 Guard for 2. (or 4.6mm ) Column (1.mm 1mm ) S3819(3/Pack) S3829(3/Pack) S3839(3/Pack) 1.mm mm - S387 - S388 - S389 2.mm mm - S S S mm 1mm guard column is a cartridge type column and needs a holder. Holder:S14 29 Tokyo Chemical Industry Co., Ltd.
20 Guard Column For 4.6mmID column Cartridge type guard column Size : 3mm 1mm For 2.mmID column or clean sample analysis Size : 1mm 1mm Holder Connector Guard Column Main Column 29 Tokyo Chemical Industry Co., Ltd.
21 Compounds of Application 1 29 Tokyo Chemical Industry Co., Ltd.
22 Compounds of Application 2 29 Tokyo Chemical Industry Co., Ltd.
SPE and HPLC. Dr Iva Chianella Lecturer in Analytical Chemistry Cranfield Health +44 (0) 1234 758322. i.chianella.1998@cranfield.ac.
SPE and HPLC Dr Iva Chianella Lecturer in Analytical Chemistry Cranfield Health +44 (0) 1234 758322 i.chianella.1998@cranfield.ac.uk Solid-Phase Extraction- SPE Simple, fast and efficient sample preparation
More informationHPLC Column Comparison Screening Study for Reversed Phase Columns
HPLC Column Comparison Screening Study for Reversed Phase Columns Carmen T. Santasania, Eric M. Snyder, Matilal Sarker, and Tracy L. Ascah Supelco, 595 North Harrison Road, Bellefonte, PA 16823, USA T403058
More informationGuide to Reverse Phase SpinColumns Chromatography for Sample Prep
Guide to Reverse Phase SpinColumns Chromatography for Sample Prep www.harvardapparatus.com Contents Introduction...2-3 Modes of Separation...4-6 Spin Column Efficiency...7-8 Fast Protein Analysis...9 Specifications...10
More informationCarbohydrate Analysis: Column Chemistries and Detection
Carbohydrate Analysis: Column Chemistries and Detection Joe Romano Waters Corporation Carbohydrates in Feeds Methodology Forum AOAC 2007 Annual Meeting Anaheim, CA September 18, 2007 2007 Waters Corporation
More informationThermo Scientific Syncronis HPLC Columns. Technical Manual
Thermo Scientific Syncronis HPLC Columns Technical Manual Thermo Scientific Syncronis HPLC Columns When developing a new method, one of the most important goals for the chromatographer is to achieve a
More informationAffi-Prep Protein A Matrix Instruction Manual
Affi-Prep Protein A Matrix Instruction Manual Catalog Numbers 156-0005 156-0006 Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547 LIT-230 Rev B Table of Contents Section 1 Introduction...1
More informationCONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY
CONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY 9.1 POLICY This test method may be used to confirm the presence of zolpidem (ZOL), with diazepam-d 5 (DZP-d 5 ) internal standard, in
More informationHigh sensitivity assays using online SPE-LC-MS/MS -How low can you go? Mohammed Abrar Unilabs York Bioanalytical solutions, York, UK
High sensitivity assays using online SPE-LC-MS/MS -How low can you go? Mohammed Abrar Unilabs York Bioanalytical solutions, York, UK Background Unilabs YBS are a bioanalytical CRO based in York (Uk) Copenhagen
More informationAnalytical Test Report
Analytical Test Report Customer: Address (City, State): Purchase Order: Report Number: Project Number: Date Received: Date of Report: Test Location: Boulder, CO. Assay: Part Number: Amino Acids by HPLC
More informationFacile HPLC Separation of Peptides: Resolution of Chiral/Achiral Single Amino Acid Analogues using Teicoplanin-based Chiral Stationary Phases
Facile PLC Separation of Peptides: Resolution of Chiral/Achiral Single Amino Acid Analogues using Teicoplanin-based Chiral Stationary Phases J.T. Lee and Thomas E. Beesley Advanced Separation Technologies
More informationAutomated Method Development Utilizing Software-Based Optimization and Direct Instrument Control
Automated Method Development Utilizing Software-Based Optimization and Direct Instrument Control Dr. Frank Steiner, 1 Andreas Brunner, 1 Fraser McLeod, 1 Dr. Sergey Galushko 2 1 Dionex Corporation, Germering,
More informationBulk Materials for Preparative and Process Chromatography
Bulk Materials for Preparative and Process Chromatography Silica Based HPLC Bulk Media Characteristics Available in both reversed and normal phases High chemical stability for low leaching High loading
More informationThermo Scientific HyperSep Solid Phase Extraction Method Development Guide
chromatography Thermo Scientific HyperSep Solid Phase Extraction Method Development Guide The following guide provides considerations, tips and general guidelines for developing SPE methods using the Thermo
More informationIAM Chromatography. HPLC Separation Tools for Membrane Protein Purification and Drug Membrane Permeability Prediction
IAM Chromatography Immobilized Artificial Membrane (IAM) technology is an innovative approach to chromatography in which the chromatographic surface emulates the lipid environment of the cell membrane.
More informationETHYL LAUROYL ARGINATE
ETHYL LAURYL ARGINATE New specifications prepared at the 69th JECFA (2008), published in FA JECFA Monographs 5 (2008). An ADI of 0-4 mg/kg bw was established at the 69th JECFA (2008). SYNNYMS DEFINITIN
More informationThermo Scientific SOLA SPE cartridges and plates Technical Guide. Join the revolution. unparalleled performance
Thermo Scientific SOLA SPE cartridges and plates Technical Guide Join the revolution unparalleled performance Join the revolution next-generation SPE Thermo Scientific SOLA products revolutionize Solid
More informationLUMEFANTRINE Draft proposal for The International Pharmacopoeia (October 2006)
October 2006 RESTRICTED LUMEFANTRINE Draft proposal for The International Pharmacopoeia (October 2006) DRAFT FOR DISCUSSION World Health Organization 2006 All rights reserved. This draft is intended for
More informationProtocol: HPLC (amino acids)
Page 1 of 6 1. Chemicals Composition M MW gram volume (ml) product code 0-pthaldialdehyde C 8H 6O 2 134.13 Sigma P0657 Perchloric acid HClO 4 3.5 100.46 Dilute 70% stock solution 1:1 with demi to obtain
More informationThe Essential CHROMacademy Guide. Mobile Phase Optimization Strategies for Reversed Phase HPLC
The Essential CHROMacademy Guide Mobile Phase Optimization Strategies for Reversed Phase HPLC Webcast Notes Type your questions in the Submit Question box, located below the slide window You can enlarge
More informationChapter 28: High-Performance Liquid Chromatography (HPLC)
Chapter 28: High-Performance Liquid Chromatography (HPLC) Scope Instrumentation eluants, injectors, columns Modes of HPLC Partition chromatography Adsorption chromatography Ion chromatography Size exclusion
More informationDiffusion and Fluid Flow
Diffusion and Fluid Flow What determines the diffusion coefficient? What determines fluid flow? 1. Diffusion: Diffusion refers to the transport of substance against a concentration gradient. ΔS>0 Mass
More informationHEXANES. Insoluble in water, soluble in ether, alcohol, and acetone. Neutral to methyl orange (ph indicator) Not more than 0.
HEXANES Prepared at the 51st JECFA (1998), published in FNP 52 Add 6 (1998) superseding specifications prepared at the 14th JECFA (1970), published in NMRS 48B (1971) and in FNP 52 (1992). ADI "limited
More informationAnalysis of Vegetable Oils by High Performance Liquid Chromatography
Analysis of Vegetable Oils by High Performance Liquid Chromatography Using Evaporative Light Scattering Detection and Normal Phase Eluents Andrew J. Aubin, Cecilia B. Mazza, Donald A. Trinite, and Patricia
More informationAn Advanced Base Deactivated Capillary Column for analysis of Volatile amines Ammonia and Alcohols.
An Advanced Base Deactivated Capillary Column for analysis of Volatile amines Ammonia and Alcohols. Jaap de Zeeuw, Ron Stricek and Gary Stidsen Restek Corp Bellefonte, USA To analyze basic compounds at
More informationApplication Note. Determination of Nitrite and Nitrate in Fruit Juices by UV Detection. Summary. Introduction. Experimental Sample Preparation
Application Note Determination of Nitrite and Nitrate in Fruit Juices by UV Detection Category Food Matrix Fruit Juice Method HPLC Keywords Ion pair chromatography, fruit juice, inorganic anions AZURA
More informationSorbents. [ A] [ A] aq. Strong affinity = large K D. Digital chromatography: all-or. or-nothing LC mechanism to extremes K =
Theory of SPE 1 SPE Nonequilibrium,, exhaustive removal of chemical constituents from a flowing liquid sample via retention on a contained solid sorbent and subsequent recovery of selected constituents
More informationSolid Phase Extraction Products PAGE: 1. Introduction of Solid Phase Extraction (SPE) Why Choose Nano-Micro Tech SPE
Solid Phase Extraction Products PAGE: 1 Introduction of Solid Phase Extraction (SPE) SPE has been used increasingly in chemical analysis and purification broadly, and become the most popular technology
More informationFast and Accurate Analysis of Vitamin D Metabolites Using Novel Chromatographic Selectivity and Sample Preparation
Fast and Accurate Analysis of Vitamin D Metabolites Using Novel Chromatographic Selectivity and Sample Preparation Craig Aurand 1, David Bell 1, Anders Fridstrom 2, and Klaus Buckendahl 2 1 Supelco, Div.
More informationProtein Separation Technology
[ ] Protein Separation Technology Protein Separation Technology The analysis and characterization of protein samples requires the detection of small chemical differences between large molecules. Most often
More informationSimultaneous determination of L-ascorbic acid and D-iso-ascorbic acid (erythorbic acid) in wine by HPLC and UV-detection (Resolution Oeno 11/2008)
Method OIV-MA-AS313-22 Type II method Simultaneous determination of L-ascorbic acid and D-iso-ascorbic acid (erythorbic acid) in wine by HPLC and UV-detection (Resolution Oeno 11/2008) 1. Introduction
More informationThermo Scientific SOLAµ SPE Plates Technical Guide. Consistent excellence. for bioanalysis
Thermo Scientific SOLAµ SPE Plates Technical Guide Consistent excellence for bioanalysis SOLAµ - delivering reproducible low volume extractions. Everytime! Thermo Scientific SOLAµ plates are designed for
More informationChapter 5 Classification of Organic Compounds by Solubility
Chapter 5 Classification of Organic Compounds by Solubility Deductions based upon interpretation of simple solubility tests can be extremely useful in organic structure determination. Both solubility and
More informationFast, Reproducible LC-MS/MS Analysis of Dextromethorphan and Dextrorphan
Fast, Reproducible LC-MS/MS Analysis of and Kimberly Phipps, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 685 Key Words Accucore C18, dextromethorphan, dextrorphan, SOLA CX Abstract
More informationEXPERIMENT 5: DIPEPTIDE RESEARCH PROJECT
EXPERIMENT 5: DIPEPTIDE RESEARCH PROJECT Pre-Lab Questions: None. 64 I. Background Information DIPEPTIDE RESEARCH PROJECT Methods developed by organic chemists for the synthesis of biopolymers have had
More informationCopyright 2010 Pearson Education, Inc. Chapter Fourteen 1
An alcohol has an OH bonded to an alkyl group; a phenol has an OH bonded directly to an aromatic ring; and an ether has an O bonded to two organic groups. Chapter Fourteen 1 Ethyl alcohol, dimethyl ether,
More informationHigh-Throughput 3-D Chromatography Through Ion Exchange SPE
High-Throughput 3-D Chromatography Through Ion Exchange SPE Application Note 205 Luke Roenneburg and Alan Hamstra (Gilson, Inc.) Introduction 2-dimensional (2-D) separation is the separation of a sample
More informationMaterials for Pharmaceutical Manufacturing
Materials for Pharmaceutical Manufacturing GRACE WHITEPAPER Vydac 150HC Purification Media Greater Loading Capacity and Resolution for Improved Productivity in Peptide Purification Technical Development:
More informationA Novel Approach to Low Volume Sample Preparation
A Novel Approach to Low Volume Sample Preparation Tony Edge, Jon Bardsley, Ken Meadows, Luisa Pereira International Symposium on Chromatography Salzburg, Austria September 2014 1 The world leader in serving
More informationSUCRALOSE. White to off-white, practically odourless crystalline powder
SUCRALOSE Prepared at the 41st JECFA (1993), published in FNP 52 Add 2 (1993). Metals and arsenic specifications revised at the 63rd JECFA (2004). An ADI of 0-15 mg/kg bw was established at the 37th JECFA
More informationSIMULTANEOUS DETERMINATION OF TELMISARTAN AND HYDROCHLOROTHIAZIDE IN TABLET DOSAGE FORM USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
CHAPTER 5 SIMULTANEOUS DETERMINATION OF TELMISARTAN AND HYDROCHLOROTHIAZIDE IN TABLET DOSAGE FORM USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY CHAPTER 5 Simultaneous determination of Telmisartan
More informationAmino Acid Analyzer L-8900
Amino Acid Analyzer L-8900 VWR - Hitachi Your Partner in Amino Acid Analysis Hitachi has manufactured more than 1800 Amino Acid Analysers for over 40 years. The new Amino Acid Analyser Model L-8900 is
More informationα-cyclodextrin SYNONYMS α-schardinger dextrin, α-dextrin, cyclohexaamylose, cyclomaltohexaose, α- cycloamylase
α-cyclodextrin New specifications prepared at the 57th JECFA (2001) and published in FNP 52 Add 9 (2001). An ADI not specified was established at the 57th JECFA (2001). SYNONYMS α-schardinger dextrin,
More informationAnalysis of the Vitamin B Complex in Infant Formula Samples by LC-MS/MS
Analysis of the Vitamin B Complex in Infant Formula Samples by LC-MS/MS Stephen Lock 1 and Matthew Noestheden 2 1 AB SCIEX Warrington, Cheshire (UK), 2 AB SCIEX Concord, Ontario (Canada) Overview A rapid,
More informationHypercarbColumns 100% porous graphitic carbon for extended separation capabilities
Columns 00% porous graphitic carbon for extended separation capabilities Exceptional retention of very polar analytes Separates structurally related substances ph stable from 0 to Ideal for high temperature
More informationPurification of reaction mixtures using flash chromatography.
Purification of reaction mixtures using flash chromatography. This technical note details the use of ISOLUTE Flash chromatography columns for the purification of reaction mixtures. What is flash chromatography?
More information- - 1 Dr. Shulamit Levin, Analytical Consulting, Medtechnica
Ion xchange Chromatography Chromatographic rocess BA Mobile phase tationary hase A Dr. hulamit Levin Medtechnica B Distribution: K = C s/cm B A lution through the Column Chromatogram Ion xchange Theory
More informationTamsulosin Hydrochloride Capsules
. nal Revision Bulletin Official October 1, 2011 Tamsulosin 1 standard solution, and shake well. Centrifuge at 1500 rpm for 10 min, and use the supernatant, passing it if Tamsulosin Hydrochloride Capsules
More informationReversed Phase High Presssure Liquid Chromatograhphic Technique for Determination of Sodium Alginate from Oral Suspension
International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.2, No.2, pp 1634-1638, April-June 2010 Reversed Phase High Presssure Liquid Chromatograhphic Technique for Determination
More informationApplications of Organic Solvent Nanofiltration in the Process Development of Active Pharmaceutical Ingredients. Dominic Ormerod
Applications of rganic Solvent Nanofiltration in the Process Development of Active Pharmaceutical Ingredients Dominic rmerod Introduction A non-thermal solvent exchange. Removal of Excess reagents via
More informationfor SolEx TM HRP Cartridges SolEx TM HRP RSLC Columns
for SolEx TM HRP Cartridges SolEx TM HRP RSLC Columns Product Manual for SolEx TM HRP Cartridges and SolEx TM HRP RSLC Columns Page 1 of 15 Product Manual for SolEx TM HRP Cartridges SolEx HRP (P/N 074400)
More informationApplication Note. Increasing the activity of monoclonal antibody isoforms by MCSGP. Summary
Application Note Increasing the activity of monoclonal antibody isoforms by MCSGP Category Matrix Method Keywords Countercurrent chromatography, FPLC Antibodies MCSGP FPLC, Biobetters, MCSGP, countercurrent
More informationLC-MS/MS Method for the Determination of Docetaxel in Human Serum for Clinical Research
LC-MS/MS Method for the Determination of Docetaxel in Human Serum for Clinical Research J. Jones, J. Denbigh, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 20581 Key Words SPE, SOLA,
More informationAFFINITY CHROMATOGRAPHY
92 AFFINITY CHROMATOGRAPHY Tosoh bioscience ANALYSIS 93 AFFINITY CHROMATOGRAPHY AFFINITY CHROMATOGRAPHY afc PRODUCTS TSKgel BORONATE-5PW TSKgel CHELATE-5PW TSKgel TRESYL-5PW TOSOH FACT The Tosoh logo symbolizes
More informationDetermining the Structure of an Organic Compound
Determining the Structure of an Organic Compound The analysis of the outcome of a reaction requires that we know the full structure of the products as well as the reactants In the 19 th and early 20 th
More informationUnique Bonding Chemistry
columns Acclaim HPLC Columns Reversed-phase silica columns for pharmaceutical, nutraceutical, environmental, food, and life science applications in HPLC. High hydrophobicity, low polarity phases LC/MS
More informationHow To Test For Contamination In Large Volume Water
Automated Solid Phase Extraction (SPE) of EPA Method 1694 for Pharmaceuticals and Personal Care Products in Large Volume Water Samples Keywords Application Note ENV0212 This collaboration study was performed
More informationENrich SEC 70 ENrich SEC 650 High-Resolution Size Exclusion Columns Instruction Manual
ENrich SEC 70 ENrich SEC 650 High-Resolution Size Exclusion Columns Instruction Manual Catalog numbers 780-1070 780-1650 Please read these instructions before you use ENrich SEC high-resolution size exclusion
More informationOasis HLB Cartridges and 96-Well Plates
CONTENTS I. INTRODUCTION II. SAMPLE PRE-TREATMENT a. Biological Samples b. Solid Samples: Soil, Whole Foods, Tissue c. Aqueous Samples: Water, Beverages d. Non-Aqueous Liquid III. SOLID PHASE EXTRACTION
More informationDevelop a Quantitative Analytical Method for low (» 1 ppm) levels of Sulfate
Cantaurus, Vol. 7, 5-8, May 1999 McPherson College Division of Science and Technology Develop a Quantitative Analytical Method for low (» 1 ppm) levels of Sulfate Janet Bowen ABSTRACT Sulfate is used in
More informationApplication Note. Purifying common light-chain bispecific antibodies using MCSGP. Summary
Application Note Purifying common light-chain bispecific antibodies using MCSGP Category Matrix Method Keywords Analytes ID Continuous chromatography, biochromatography Antibodies MCSGP Bispecific antibody,
More informationMass Spec - Fragmentation
Mass Spec - Fragmentation An extremely useful result of EI ionization in particular is a phenomenon known as fragmentation. The radical cation that is produced when an electron is knocked out of a neutral
More informationApplication Note. Determination of Amino acids by UHPLC with automated OPA- Derivatization by the Autosampler. Summary. Fig. 1.
Application Note Determination of Amino acids by UHPLC with automated PA- Derivatization by the Autosampler Category Bio Analysis Matrix - Method UHPLC Keywords Proteinogenic Amino acids, Canonical Amino
More informationß-CYCLODEXTRIN SYNONYMS
ß-CYCLODEXTRIN Prepared at the 44th JECFA (1995), published in FNP 52 Add 3 (1995) superseding specifications prepared at the 41st JECFA (1993), published in FNP 52 Add 2 (1993). Metals and arsenic specifications
More informationEXAMINATION IN COURSE KJ 2053 CHROMATOGRAPHY
NTNU Norwegian University of Science and Technology Faculty of Natural Sciences and Technology Department of chemistry Page 1 (of 13) Inquiries concerning the contents of the test to : Assoc. professor
More information[ Care and Use Manual ]
ACQUITY UPLC CSH Columns Contents I. Getting started a. Column Connectors b. Column Installation c. Column Equilibration d. ecord Installation e. Initial Column Efficiency Determination f. VanGuard Pre-Columns
More informationAnalysis of Fat-Soluble Vitamin Capsules using UltraPerformance Convergence Chromatography (UPC 2 )
Analysis of Fat-Soluble Vitamin Capsules using UltraPerformance Convergence Chromatography (UPC 2 ) Andrew Aubin Waters Corporation, Milford, MA, USA A P P L I C AT ION B E N E F I T S Fast analysis of
More informationPeptide synthesis, radiolabelling and radiochemical analysis
SUPPLEMENTAL DATA MATERIALS AND METHODS Peptide synthesis, radiolabelling and radiochemical analysis Solid phase synthesis of peptides was carried out on using ABI 433A peptide synthesizer, on a preloaded
More informationAPPLICATIONS MANUAL. Ifosfamide in blood serum... 2. Ingredients in blood serum... 3. Organophosphorus pesticides in tea leaf... 5
1 APPLICATIONS MANUAL CONTENTS Ifosfamide in blood serum... 2 Ingredients in blood serum... 3 Organophosphorus pesticides in tea leaf... 5 Sudan i ii iii iv in chilli sauce... 6 Pah in water... 7 Phenols
More informationPipette Tips PACKARD BIOSCIENCE MultiPROBE II COMPATIBLE
User Guide for Reversed-Phase Pipette Tips PACKARD BIOSCIENCE MultiPROBE II COMPATIBLE Notice The information in this document is subject to change without notice and should not be construed as a commitment
More informationDrospirenone and Ethinyl Estradiol Tablets. Type of Posting. Revision Bulletin Posting Date. 31 July 2015 Official Date
Drospirenone and Ethinyl Estradiol Tablets Type of Posting Revision Bulletin Posting Date 31 July 2015 Official Date 01 August 2015 Expert Committee MonographsSmall Molecules 4 Reason for Revision Compliance
More informationPreliminary MFM Quiz
Preliminary MFM Quiz 1. The major carrier of chemical energy in all cells is: A) adenosine monophosphate B) adenosine diphosphate C) adenosine trisphosphate D) guanosine trisphosphate E) carbamoyl phosphate
More informationVALIDATION OF HPLC METHOD FOR DETERMINATION OF DOCOSAHEXAENOIC ACID (DHA) IN MULIIVITAMIN CAPSULE DIAH WIDOWATI*, ROS SUMARNY, ESTI MUMPUNI
VALIDATION OF HPLC METHOD FOR DETERMINATION OF DOCOSAHEXAENOIC ACID (DHA) IN MULIIVITAMIN CAPSULE DIAH WIDOWATI*, ROS SUMARNY, ESTI MUMPUNI Fakultas Farmasi Universitas Pancasila Jln. Srengseng Sawah,
More informationApplication Note. Separation of three monoclonal antibody variants using MCSGP. Summary
Application Note Separation of three monoclonal antibody variants using MCSGP Category Matrix Method Keywords Analytes ID Continuous chromatography, Biochromatography; FPLC Protein A-purified monoclonal
More informationThe Automated SPE Assay of Fipronil and Its Metabolites from Bee Pollen.
The Automated SPE Assay of Fipronil and Its Metabolites from Bee Pollen. Mark Crawford Single Presenter Name Abstract With the recent decline in hive bee populations this year the investigation of acaricides
More informationCH 102 Practice Exam 2 PCC-Sylvania
CH 102 Practice Exam 2 PCC-Sylvania True/False Indicate if the statement is true or false. 1.Tertiary alcohols are not easily oxidized. 2.Secondary alcohols can be oxidized to aldehydes. 3.Primary alcohols
More informationNew drugs of abuse? A case study on Phenazepam & Methoxetamine
New drugs of abuse? A case study on Phenazepam & Methoxetamine Presenter: Nadia Wong Co authors: Dr Yao Yi Ju & Alex Low Xuan Kai Analytical Toxicology Laboratory Clinical & Forensic Toxicology Unit Applied
More information[ Care and Use Manual ]
Analytical columns and cartridges i. Introduction Waters offers a large selection of analytical columns and cartridges packed with silica-based packing materials. Follow this instruction sheet to obtain
More informationAcids and Bases. but we will use the term Lewis acid to denote only those acids to which a bond can be made without breaking another bond
Acids and Bases. Brønsted acids are proton donors, and Brønsted bases are proton acceptors. Examples of Brønsted acids: HCl, HBr, H 2 SO 4, HOH, H 3 O +, + NH 4, NH 3, CH 3 CO 2 H, H CH 2 COCH 3, H C CH,
More informationSPE Reference Manual & Users Guide. Reference Manual & Users Guide
SM SPE Reference Manual & Users Guide SPE Reference Manual & Users Guide Reference Manual & Users Guide SPE Table of Contents Page No. 1. Objective and Purpose of This Guide 1 2. Solid Phase Extraction
More informationALCOHOLS: Properties & Preparation
ALLS: Properties & Preparation General formula: R-, where R is alkyl or substitued alkyl. Ar-: phenol - different properties. Nomenclature 1. ommon names: Name of alkyl group, followed by word alcohol.
More informationANALYSIS OF FOOD AND NATURAL PRODUCTS LABORATORY EXERCISE
ANALYSIS OF FOOD AND NATURAL PRODUCTS LABORATORY EXERCISE Determination of carbohydrates in foodstuff (LC/RID method) Exercise guarantor: Assoc. Prof. Karel Cejpek, Ph.D. CONTENT Required knowledge...
More informationUltraviolet Spectroscopy
Ultraviolet Spectroscopy The wavelength of UV and visible light are substantially shorter than the wavelength of infrared radiation. The UV spectrum ranges from 100 to 400 nm. A UV-Vis spectrophotometer
More informationGel Filtration Standard
Gel Filtration Standard Instruction Manual Catalog # 151-1901 Table of Contents Section 1 Introduction... 1 1.1 Instructions... 1 1.2 Recommended Volume of Standard... 2 1.3 Shelf Life... 5 1.4 Storage...
More informationHow To Use An Acquity Qda Detector
Mass-Directed Isolation of a Synthetic Peptide Using the ACQUITY QDa Detector Jo-Ann M. Jablonski and Andrew J. Aubin Waters Corporation, Milford, MA, USA APPLICATION BENEFITS The ACQUITY QDa Detector
More informationPaper interaction with fountain solution during multi-color offset printing
Paper interaction with fountain solution during multi-color offset printing F. Ciolacu, Elena Bobu PULP, PAPER AND PRINTING DEPARTMENT GH.ASACHI TECHNICAL UNIVERSITY OF IASI ROMANIA Offset printing Printing
More informationChia-Chuan Chang, Pennaka Hari Kishore, Shoei-Sheng Lee*
Chemical investigation of Phyllanthus maderaspatensis by hyphenation of liquid chromatography-solid phase extraction-nuclear magnetic resonance spectroscopy Chia-Chuan Chang, Pennaka Hari Kishore, Shoei-Sheng
More informationSupporting Information. Minimum active structure of insulin-like. peptide 5 (INSL5)
Supporting Information Minimum active structure of insulin-like peptide 5 (INSL5) Alessia Belgi 1,2, Ross A.D. Bathgate *1,2,3, Martina Kocan *4, Nitin Patil 1,5, Suode Zhang 1, Geoffrey W. Tregear 1,2,
More informationInfrared Spectroscopy 紅 外 線 光 譜 儀
Infrared Spectroscopy 紅 外 線 光 譜 儀 Introduction Spectroscopy is an analytical technique which helps determine structure. It destroys little or no sample (nondestructive method). The amount of light absorbed
More informationStandard Analytical Methods of Bioactive Metabolitesfrom Lonicera japonica Flower Buds by HPLC-DAD and HPLC-MS/MS
Standard Analytical Methods of Bioactive Metabolitesfrom Lonicera japonica Flower Buds by HPLCDAD and HPLCMS/MS Jongheon Shin College of Pharmacy Seoul National University Summary The standard analytical
More informationMethod Development for Size-Exclusion Chromatography of Monoclonal Antibodies and Higher Order Aggregates
Method Development for Size-Exclusion Chromatography of Monoclonal Antibodies and Higher Order Aggregates Paula Hong and Kenneth J. Fountain Waters Corporation, 34 Maple St., Milford, MA, USA APPLICATION
More informationLecture Overview. Hydrogen Bonds. Special Properties of Water Molecules. Universal Solvent. ph Scale Illustrated. special properties of water
Lecture Overview special properties of water > water as a solvent > ph molecules of the cell > properties of carbon > carbohydrates > lipids > proteins > nucleic acids Hydrogen Bonds polarity of water
More informationReversed phase C-18 chromatography: From analytical scale to preparative scale
Reversed phase C-18 chromatography: From analytical scale to preparative scale Veronica Thomason*, Jeffrey V. Mitten, and Paul Bellinghausen Teledyne Isco, Inc. P.O. Box 82531 Lincoln, NE 68501 2 Abstract
More informationAdvantages of Polar, Reversed- Phase HPLC Columns for the Analysis of Drug Metabolites
Advantages of Polar, Reversed- Phase HPLC Columns for the Analysis of Drug Metabolites Carmen T. Santasania and David S. Bell SUPELCO, 595 North Harrison Road, Bellefonte, PA 16823 T404045 GUS Abstract
More informationSimultaneous determination of aspartame, benzoic acid, caffeine, and saccharin in sugar-free beverages using HPLC
Simultaneous determination of aspartame, benzoic acid, caffeine, and saccharin in sugar-free beverages using HPLC Mackenzie Ree and Erik Stoa Department of Chemistry, Concordia College, 901 8 th St S,
More informationPrepTip. Reverse Phase PrepTip User Guide
PrepTip Reverse Phase PrepTip User Guide All text, photographs and illustrations are copyrighted by Harvard Apparatus, Inc. 2004. PrepTip is a trademark of Harvard Apparatus, Inc. Harvard Apparatus 84
More informationSize Exclusion Chromatography
Size Exclusion Chromatography TOYOPEARL Resins for SEC TOYOPEARL TOYOPEARL TOYOPEARL HW-55 TOYOPEARL HW-65 TOYOPEARL TOSOH BIOSCIENCE TOSOH BIOSCIENCE LLC 56 Keystone Drive Montgomeryville, PA 896-967
More informationBuy your Flash columns from a company that actually manufactures the packings. Get consistent results because there s uniformity from batch to batch.
Disposable Columns for Flash Chromatography Spe-ed Flash columns have been designed and manufactured to meet the critical needs of researchers who are using automated organic purification instruments.
More informationOrganic Functional Groups Chapter 7. Alcohols, Ethers and More
Organic Functional Groups Chapter 7 Alcohols, Ethers and More 1 What do you do when you are in Pain? What do you do when you are in a lot of pain? 2 Functional Groups A functional group is an atom, groups
More information