7 th SENFTENBERGER INNOVATIONSFORUM MULTIPARAMETERANALYTIK
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1 7 th SENFTENBERGER INNOVATIONSFORUM MULTIPARAMETERANALYTIK April 18 19, 2013 Hochschule Lausitz (FH) Senftenberg Germany
2 Organized by: In cooperation with: Supported by:
3 7 th Senftenberger Innovationsforum Multiparameteranalytik April 18 19, 2013 Hochschule Lausitz (FH) Senftenberg Trends in Microanalytical Systems and Chip-based Bioanalysis The forum Trends in Microanalytical Systems and Chip-based Bioanalysis will be organized by Lausitz University of Applied Sciences, BioResponse e. V. together with the Center for Molecular Diagnostics and Bioanalysis (ZMDB) as the 7. Senftenberger Innovationsforum Multiparameteranalytik. The scope of the workshop comprises microanalytical systems and chip-based bioanalysis with a focus on the real-time monitoring of amplification reactions and related multiplex technologies. Das 7. Senftenberger Innovationsforum Multiparameteranalytik Trends in Microanalytical Systems and Chip-based Bioanalysis wird von der Hochschule Lausitz (FH), dem BioResponse e.v. und dem Zentrum für Molekulare Diagnostik und Bioanalytik (ZMDB) veranstaltet. Die Veranstaltung präsentiert aktuelle Entwicklungen im Bereich der mikroanalytischen Systeme und chipbasierten Bioanalytik. Der thematische Fokus liegt auf der Echtzeitanalyse von Amplifikationsreaktionen und verwandten Multiplextechnologien. Accompanying Exhibitions: Posters, scientific demonstrators as well as industrial products and services will be presented. Call for papers Selected papers will be published in a special issue of Microchimica Acta (Springer). The selection will be performed by peer review procedure. Conference language: English Participation is free of charge. Prior registration and further details are available at:
4 7 th Senftenberger Innovationsforum Multiparameteranalytik April 18 19, 2013 Hochschule Lausitz (FH) Senftenberg Programme April 18 Welcome Adress [Chair: Günter Peine] 9:30 Günter Schulz [Lausitz University of Applied Sciences Senftenberg] 9:35 Hans-Peter Hiepe [Federal Ministry of Education and Research (BMBF) Berlin] Key Note Lectures Chip-based Amplification [Chair: Werner Lehmann] 10:00 The use of -omic technologies for transcriptional biomarker development Michael W. Pfaffl [Technische Universität München Munich] 10:40 Coffee 11:00 New luminescent sensing materials for microfluidic applications Ingo Klimant [Graz University of Technology Graz] Chip-based Amplification [Chair: Christine Mißler] 11:40 Multiplex microbead real-time PCR with microscope imaging technology read-out. Stefan Rödiger [Lausitz University of Applied Sciences Senftenberg] 12:05 Videoscan 2.0 a new bioanalytical platform Werner Lehmann [Attomol GmbH Bronkow] 12:30 Miniaturized diagnostic systems for infectious disease control and pathways to the market Dirk Kuhlmeier [Fraunhofer Institute for Cell Therapy and Immunology IZI Leipzig] 12:55 Microarray and microfluidic based point of care diagnostics Ralf Ehricht [Alere Technologies GmbH Jena] 13:20 Poster Exhibition and Lunch
5 Real-Time PCR LoopTag for Mutation and Pathogen Detection ATTOMOL GmbH was founded in 1997 as a biochemically and molecular biologically focused company in the South of Brandenburg. We develop and produce medical diagnostic tests for the business segments of molecular genetics, infectious and autoimmune diseases. Assay features: melting curve analysis equal PCR protocols less time and labour adapted for LightCycler Mutation Detection Assays Factor II 20210G>A Factor V Leiden MTHFR 677C>T, 1298A>C Lactose intolerance C>T, G>A Melting curve analysis for identification of mutations: Genotypes homozygous wild type (blue) heterozygous (green) homozygous mutation (red) -(d/dt)fluorescence (640/530) 0,04 0,035 0,03 0,025 0,02 0,015 0,01 0, ,005-0,01-0, Temperature ( C) Pathogen Detection Assays Borrelia burgdorferi (sensu lato) Mycobacterium tuberculosis complex B. pertussis / B. parapertussis Amplification curves indicate different amounts of pathogens in the samples.
6 7 th Senftenberger Innovationsforum Multiparameteranalytik April 18 19, 2013 Hochschule Lausitz (FH) Senftenberg Programme April 18 Speed Lectures [Chair: Stefan Rödiger] 14:40 Isothermal nucleic acid amplification as an alternative approach for molecular point of care testing Sebastian Kersting [Fraunhofer Institute for Biomedical Engineering IBMT Potsdam] 14:50 Selection and validation of qpcr reference genes examplified for PBMCs in vaccine development Lars Radke [University of Applied Sciences Wildau Wildau] 15:00 Simultaneous detection of anti-neutrophil cytoplasmic antibodies and reactivity to corresponding antigenic targets by a novel indirect immunofluorescence test Mandy Sowa [Lausitz University of Applied Sciences Senftenberg] 15:10 Coffee Poland as Partner in Research and Development [Chair: Wolfgang Korek] 15:40 HPV analysis in head and neck cancer along with precancerous conditions various methods and algorithms Agata Jósefiak [Centrum Badan DNA sp. z o.o. Poznan] 16:00 Complex problems - multiparameter solutions: No better way? Jurek Superata [AfiSen sp. z o.o. Krakow] Evening Lecture and Get Together 16:10 Günter Schulz [Lausitz University of Applied Sciences Senftenberg] 16:20 Twenty years of microfluidic PCR: An academic curiosity or indispensable tool? Andrew demello [Swiss Federal Institute of Technology Zurich] 17:00 Get Together
7 KLIDES Platform technology for multiplex diagnostics using digital multicolor immunofluorescence MEDIPAN GMBH Ludwig-Erhard-Ring Dahlewitz Tel: FAX: info@medipan.de
8 7 th Senftenberger Innovationsforum Multiparameteranalytik April 18 19, 2013 Hochschule Lausitz (FH) Senftenberg Programme April 19 Keynote Lecture 09:00 Molecular diagnostics of infectious diseases Frank T. Hufert [University Medical Center Göttingen Göttingen] MEDIPAN Lecture: Biomolecular Applications 09:40 MicroRNA profiling as a new tool in personalized medicine Dirk Laßner [Institut Kardiale Diagnostik und Therapie GmbH Berlin] Biomolecular Applications [Chair: Dirk Roggenbuck Peter Schierack] 10:10 Multiplex SNP genotyping of tumorcell DNA for KRAS mutations by allele specific real-time PCR on a centrifugal microfluidic disk segment "GeneSlice" Oliver Strohmeier [Institut für Mikro- und Informationstechnik HSG-IMIT Villingen-Schwenningen] 10:35 Surface-functionalized fluorescent particles as tools for bioanalytics Characterization strategies and different applications Ute Resch-Genger [Federal Institute for Materials Research and Testing (BAM) Berlin] 11:00 Coffee 11:30 Innovative chip-based technologies for bioanalytical applications Karina Weber, Dana Cialla [Friedrich-Schiller University Jena and Institute of Photonic Technology Jena] 11:55 The ne[x]d Project, an automated Lab-on-a-Chip system for molecular diagnostics of infectious diseases Ralf Himmelreich [IMM Institut für Mikrotechnik Mainz GmbH Mainz] 12:20 Cost-effective geometric multiplex mediator probe PCR Simon Wadle [Institut für Mikro- und Informationstechnik HSG-IMIT Villingen-Schwenningen] Closing Remarks 12:45 Christian Schröder [Lausitz University of Applied Sciences Senftenberg] Werner Lehmann [Attomol GmbH Bronkow]
9 REAL-TIME PCR PROBES YOU CAN RELY ON More than 20 years experience in DNA synthesis! Large selection of dye combinations suitable for Real-time PCR 5 -, 3 - or internal labels High purity probes and thorough quality control We were always fully satisfied with the exceptional quality, purity and reliability of the amount of unlabeled and labeled oligonucleotides as well as with the customer support provided by IBA. Prof. Dr. Markus Sauer, Biotechnology & Biophysics, University Würzburg, Germany. Our conference special: Real-time PCR T-scale (Test scale) for EUR 49 only*! Visit our booth at the 7th Senftenberger Innovationsforum and test our quality! * details at the booth or at oligo@iba-lifesciences.com IBA GmbH Rudolf-Wissell-Str Goettingen Germany Phone Fax oligo@iba-lifesciences.com
10 7 th Senftenberger Innovationsforum Multiparameteranalytik April 18 19, 2013 Hochschule Lausitz (FH) Senftenberg Scientific Exhibition Presentation of Demonstrators from Science AKLIDES Platform Technology The AKLIDES system is a platform technology for the multiplex diagnostics using digital multi-color immunofluorescence. It represents the world's first system for the automatic and objective analysis of immunofluorescence testing in routine diagnostics. There also exists an application for the automatic determination of DNA double-strand breaks via γh2ax foci. Fraunhofer ivd-platform The ivd-platform is a system, which enables measurements of diagnostic parameters directly at the patient's bedside. The system contains a credit card-sized cartridge and a base-unit. For usage, a drop of blood has to be applied to the cartridge, which will then be inserted into the base unit. After 15 minutes the results are displayed, enabling a fast therapeutic decision. Ne[X]D-System The ne[x]d Project resembles an automated Lab-on-a-Chip system for molecular diagnostics of infectious diseases. The system consists of a small size processing device and a microfluidic-based disposable cartridge which integrates the whole sample processing chain from sample extraction and amplification to final pathogen detection. A proof of the entire concept was shown for detection of Neisseria/Chlamydia infections and MRSA infections.
11 VideoScan Technology VideoScan 1.1 is a highly versatile fluorescence microscopic platform for the multiplex real-time detection of biomolecules. Currently, up to 8 cavities can be used for tracking of changes under dynamic heating conditions, which makes it a useful tool for multiplex PCR applications, nucleic acid hybridization and melting curve analysis. Poland as Partner in Research and Development DNA Research Center Ltd. (DNARC) DNARC is an innovative biotechnology company creating and providing services in genetic medical diagnostics, microbiology and veterinary domains, based on modern molecular technologies (NGS, microarray, real-time PCR, LAMP). Afisen Present activities focus on the development of innovative chemosensors and biosensors. We are interested in partnership in R&D projects, especially in the biomedical field, concerning bio-sensors, sepsis, oxidative stress and antioxidative defence.
12 Contact TSB Innovationsagentur Berlin GmbH Zentrum für Molekulare Diagnostik und Bioanalytik (ZMDB) Dr. Günter Peine Director Management and Coordination r z hahne@zmdb.de Veranstaltungsort Venue Hochschule Lausitz (FH) Konrad-Zuse-Bau Großenhainer Straße Senftenberg Germany Anfahrt Directions Weitere Informationen Further Information The Center for Molecular Diagnostics and Bioanalysis (ZMDB) is a Flagship Project of the Joint Innovation Strategy Berlin-Brandenburg at the TSB Innovationsagentur Berlin GmbH. The ZMDB is funded by the states of Berlin and Brandenburg and co-financed by the European Regional Development Fund (ERDF) of the European Union. An investment in your future!
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