Technical Note. Roche Applied Science. No. LC 19/2004. Color Compensation

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "Technical Note. Roche Applied Science. No. LC 19/2004. Color Compensation"

Transcription

1 Roche Applied Science Technical Note No. LC 19/2004 Purpose of this Note Color The LightCycler System is able to simultaneously detect and analyze more than one color in each capillary. Due to overlap of the emission spectra of the dyes, one channel picks up signals (crosstalk) from a dye measured by another channel. This bleed-over of fluorescence signal can result in data that cannot be interpreted. To correct the bleed-over, color compensation can be applied either during an experimental run or before data analysis. When color compensation is activated, LightCycler algorithms use the data from a so-called ccc file (LightCycler SW 3.5) or ccc object (LightCycler SW 4.0) to compensate for the fluorescence crosstalk. This Technical Note will show you how to create color compensation for dual color analyses with LC Red 640 and LC Red 705. In addition, it describes the color compensation procedure for multiplex assays. Note: Color compensation for multiplex assays can only be done on the LightCycler 2.0 Instrument. Table of Contents Basic Information about Color 2 Generating Color 4-6 Color for Dual Color Applications that Use LightCycler Red 640 and LightCycler Red 705 Creating Color Files with SW 3.5 Creating Color Objects with SW 4.0 Color for Customized Applications 7 Applications Using Customized Dual Color Hybridization Probes 8 Multicolor Hybridization Probe Applications 8-10 Application Example 11 Note: Methods of color compensation for Dual Color Hydrolysis Assays (conventionally called TaqMan Assays) will be found in Technical Note No. LC 21, Hydrolysis Probe ( TaqMan ) Assays with the LightCycler System.

2 1. Basic Information about Color Dyes and Detection Channels Spectral Overlap The LightCycler System can detect two or more different sequences in one sample, e.g., both a target and an internal control. Such assays require two specific sets of hybridization probes, each labeled with different FRET (Fluorescence Resonance Energy Transfer) acceptor dyes. Example: For dual color detection, one of the two hybridization probe sets uses LightCycler Red 640 as acceptor dye; the other uses LightCycler Red 705. Both acceptor dyes are excited by the donor dye fluorescein. These red dyes have different emission spectra, which allows the LightCycler Instrument to detect the distinctive signal from each set in different fluorimeter channels. The LightCycler 2.0 Instrument has additional detection channels that make it possible to perform multicolor (e.g. multiplex hybridization probe) assays with four different acceptor dyes: LightCycler Red 610, LightCycler Red 640, LightCycler Red 670 and LightCycler Red 705. Note: For more details of hybridization probe chemistry please read Technical Note No. LC 18/2004, Assay Formats for Use in Real-Time PCR. Although the optical filters of each detection channel are optimized for different emission maxima, all fluorescent dyes currently available have emission spectra with long tails, leading to a spectral overlap. As depicted in Figure 1, the emission spectrum of the donor dye (fluorescein) slightly overlaps the emission spectrum of LightCycler Red 640, which, in turn, overlaps the LightCycler Red 705 emission spectrum. Figure 1: Overlap of emission spectra from fluorescent dyes used in the LightCycler System. The gray vertical bars in Figure 1 show the width of the detection windows in the 530 nm, 640 nm and 705 nm channels of the LightCycler. The hatched areas below these bars represent the bleed-over (overlap) of the fluorescein and LightCycler Red 640 signals into other channels. There is especially significant overlap of the 640 nm signal into the 705 nm channel. Calibration with Color 2 You can easily correct the spectral overlap described above by calibrating the instrument with the LightCycler Color Set (Cat. No , for dual color applications that use LightCycler-Red 640 and LightCycler-Red 705) or by customized calibration (for applications that use the additional channels of the LightCycler 2.0 Instrument). During a calibration run, the LightCycler Instrument measures the fluorescence of each dye in all channels and generates an instrument-specific color-compensation file (or object). Later, the software automatically uses this so-called ccc file or ccc object to reassign the fluorescence in each channel to the appropriate dye. The net result is detection of only one dye signal in each channel. Note: Color compensation is only necessary when you run an experiment with two or more different dyes in one capillary. Color compensation is not required when the experiment uses only a single dye (e.g., SYBR Green I or LightCycler Red 640).

3 1. Basic Information about Color continued Using Factors Influencing the Relative Crosstalk Compensating for Temperature Compensating for Optical System Minimizing Effects of Coupling Chemistry Color compensation files or objects are required for accurate data analysis of dual color/multicolor assays. They may also be used to correct on-line data display during the LightCycler Instrument run. The following factors strongly influence the degree of crosstalk: temperature properties of the optical system coupling chemistry of the fluorescence dyes On the other hand, neither the final reaction volume nor any of the components of LightCycler reagents and kits that are optimized for PCR and RT-PCR significantly influence crosstalk. Note: For multicolor applications with more than two dyes and applications with adjacent fluorescent dyes (e.g. LightCycler Red 640 combined with LightCycler Red 670), the oligonucleotide sequence of the probes can influence (and usually increase) crosstalk. Thus, we recommend customized, assay-specific color compensation runs for such assays. The intensity and the spectrum of emissions from a fluorescent dye are strongly temperature dependent. Thus the temperature chosen for data acquisition (fluorescent measurement) during a PCR run significantly influences the crosstalk. This is particularly important during analysis of data measured at different temperatures (e.g., during a melting curve analysis). To provide crosstalk compensation at all temperatures, a color compensation calibration run must include a temperature gradient step (40 C 95 C) in which data from all fluorescent dyes are continuously measured in all channels. Optical systems (containing e.g. several optical filters, lenses and dichroic mirrors) will always show minor variations from instrument to instrument. This is a property of all optical systems, not just the optics in the LightCycler Instrument. If you look at raw data rather than calculated data, you can see these minor variations. Moreover, these hardware variations influence crosstalk values. Therefore, you must generate and store an instrument-specific color compensation file before starting multicolor applications on that LightCycler Instrument. The chemistry used to couple fluorescent dyes to probes can influence crosstalk. To minimize these effects, use specific labeling reagents that are available from Roche Applied Science, e.g. LightCycler Fluorescein CPG. Alternatively, if you let licensed oligonucleotide synthesis companies make your labeled probes, they will use these same high quality labeling reagents. Note: For details, see Biochemica No. 1/2001, p

4 2. Generating Color Different Possibilities for Color Color Run You can create an instrument-specific color compensation file (or object) for: Dual color applications that use LightCycler Red 640 and LightCycler Red 705 Note: Prepare this ccc file/object with the ready-to-use LightCycler Color Set. The LightCycler Software provides pre-defined protocols for this color compensation run. For details, see sections and Customized dual color applications that use HybProbe probes labeled with other dye combinations Customized multicolor applications (e.g., multiplex PCR or multicolor detection) Note: Applications involving dyes other than Red 640 and Red 705 can only be used in the LightCycler 2.0 Instrument. The temperature profile used in a color compensation protocol always includes a Heating Step, a Cycling Step, a Temperature Gradient Step and a Cooling Step. The cycling step mimics a typical PCR, including data acquisition. However, the data required for color compensation are taken from the temperature gradient step. In this step, after a brief denaturation (95 C), the protocol slowly increases the temperature, in increments of 0.2 /sec, from 40 C to 95 C while continuously acquiring data (see Fig. 2). After the calibration run, the LightCycler Software saves the data generated as a normal experimental file. For these data to be used for color compensation, you must first convert the data into a ccc file/object and save it again. Figure 2: Temperature profile that includes a temperature gradient step 4

5 2.1 Color for Dual Color Applications that Use LightCycler Red 640 and LightCycler Red 705 Overview: Dual Color with the Color Set The following sections describe how to prepare a color compensation file for a dual color experiment (using LC 640 and LC 705) on two different versions of the LightCycler Software, SW 3.5 and SW 4.0. You only need to use the LightCycler Color Set once to generate a color compensation file or color compensation object that can be used for all dual color (LC 640/LC 705) experiments on that instrument. The only times you need to generate a new ccc file/object are when you update the software or after the optical system has been repaired. The set contains four vials of ready-to-use solutions: Vial 1: standard buffer Vial 2-4: standard oligonucleotides labeled with either fluorescein (3 end; Vial 2), LightCycler Red 640 (5 end; Vial 3) or LightCycler Red 705 (5 end; Vial 4) Creating Color Files with SW 3.5 Running a Color Experiment Creating Color Files with SW 3.5 For details on running the color compensation experiment, see the pack insert of the LightCycler Color Set (new Cat. No ) and the LightCycler Operator s Manual. To perform the color compensation calibration experiment, use the calibration.exp file, which you can find in the Demo Protocol folder under Demo Data. It contains all the necessary sample information. After the calibration run, the LightCycler software automatically displays the LightCycler Data Analysis module. To convert the acquired data: Use the Select a Program function to select the melting curve segment (segment 3) of the temperature profile. (On the display, the software will place green cursors around the temperature gradient step.) Then select Calibration from the pull-down Color menu. Save the Color data by naming the calibration file and placing it in the proper folder. (The software automatically adds the.ccc extension to the file.) 5

6 2.1.1 Creating Color Files with SW 3.5 continued Applying Color Files You can activate the stored color compensation file (*.ccc file) at several points in the LightCycler Protocol to provide color compensation for raw data: On-line color compensation You can load the *.ccc file during a run on the LightCycler Instrument to correct the on-line display of data. Just click on Choose CCC File and select the appropriate file. Color compensation for data analysis You can load and activate the *.ccc file to compensate for crosstalk between channel 2 and channel 3 during dual color analysis. Before starting this analysis, just select Load Calibration Data on the Color menu, then select the appropriate file Creating Color Objects with SW 4.0 Running a Color Experiment Creating Color Objects with SW 4.0 For details on running the color compensation experiment, see the pack insert of the LightCycler Color Set (new Cat. No ) and the LightCycler Operator s Manual. To create the color compensation object, use the macro for automatic execution of the calibration, which is included in the Roche folder under Templates and Macros / Experiment Macros / Demo Macros / Color Set (Cat No ). Follow the instructions provided by the Experiment Kit Wizard. After the calibration run, select Color from the Analysis menu and save the data as a ccc object: Click the Save CC Object button. Place the object in the Special Data\CCC folder under your user name. 6

7 2.1.2 Creating Color Objects with SW 4.0 continued Applying Color Objects You can apply the stored color compensation object to all dual color HybProbe experiments which use the same dye combination (Red 640/Red 705) by doing the following: Click on Color in either the Run or the Analysis module, then choose the appropriate object under Select Color. When a dialog box opens and displays all compensated channels, click OK. Note: Do not deselect the pre-defined channels. Uncompensated channels in an experimental data set are not displayed during analysis. Result: The analysis graphs will now be redrawn using the compensated data. Notice that the Color menu label now says On. 2.2 Color for Customized Applications Introduction LightCycler 2.0 Instrument Properties You will need to develop a customized color compensation object if you want to perform an assay with: a HybProbe pair that uses different fluorophores (e.g. LC Red 610/LC Red 670) than those in the LightCycler Dual Color Set, or more than two colors of fluorophore. (Most Roche parameter-specific assay kits that use multicolor analysis include color compensation reagents that can be used to develop a customized object.) Due to assay requirements (e.g., 6-channel photometer), you must generate these compensation objects on the LightCycler 2.0 Instrument. The photometer in the LightCycler 2.0 Instrument can detect 6 different colors in distinct channels. The spectral distance of these channels is approx. 30 nm, much narrower than the nm distance in 3-channel LightCycler Instruments. Consequently crosstalk between channels is significantly higher; there may be signal bleed-over not only from a short wavelength into a longer wavelength, but also vice-versa (see Figure 3). Figure 3: Crosstalk between emission spectra on the 6-channel photometer 7

8 2.2 Color for Customized Applications continued Factors Influencing the Relative Crosstalk Values A multicolor experiment demands more stringent color compensation since it involves more colors and the distances between emission peaks of the fluorescent dyes are much shorter. In such specific applications crosstalk is also affected by the oligonucleotide sequences of the probes. However, as with dual color assays, the major factors influencing relative crosstalk values for multicolor experiments are: temperature properties of the optical system coupling chemistry of fluorescence dyes Applications Using Customized Dual Color Hybridization Probes Comments on Customized Dual Color HybProbe Assays Dual color hybridization probe assays usually use the combination of LC Red 640 and LC Red 705 fluorophores. For these, you can use a universal color compensation file or object created with the LightCycler Color Set. However, if you are using dual color assays with any other fluorophores (e.g., with LC Red 610 and LC Red 670), you must develop a color compensation object by the same procedure you would use for multicolor applications (as detailed below). Since this is a time-consuming process, we do not recommend customizing dual color hybridization probe assays. Instead, just use the LC Red 640/LC Red 705 dye combination Multicolor Hybridization Probe Applications Introduction Setting up a Multicolor Experiment If you use a multicolor application (e.g., multiplex PCR or multicolor detection), you must generate a parameter-specific color compensation object for each experiment, since the emission spectra are close to each other and can be influenced by each parameter. This is true for Roche Kits (which include reagents for color compensation) as well as for your own applications. Note: Color compensation is independent of final volume. If you need a color compensation object for an experiment with 100 µl samples, you can still use a 20 µl capillary to do the compensation experiment. To minimize the work involved, we recommend that you use the same probes in the color compensation experiment that you will use for detecting and analyzing the experimental samples. For customized multicolor compensation prepare capillaries containing: Blank For the blank, you can use water, but we highly recommend using a buffer (50 mm Tris, ph 8.3, with 0.25 mg/ml BSA). This buffer is also suitable for diluting and storing the primers and probes. Fluorescein-labeled probes as calibrator The final concentration of fluorescein-labeled probes in this capillary should be µm; use an equimolar mixture of all the different fluorescein-labeled probe sequences. 8

9 2.2.2 Multicolor Hybridization Probe Applications continued LC-Red-labeled probes as calibrators Each LC-Red-labeled probe should have its own capillary. (If you have more than one probe with the same LC-Red dye, use an equimolar mixture of all those probes in the same capillary.) Due to absence of a FRET partner, the signal intensity of the probe will be low. Therefore, use a final probe concentration of at least 1 µm. Note: You can include up to 6 capillaries (blank, Fluorescein, LC Red 610, LC Red 640, LC Red 670 and LC Red 705) in the run. Special Considerations Creating Color Objects Since the spectral distance between channels in the LightCycler 2.0 Instrument is short, channels might, in rare cases, pick up faint signals from neighboring channels, even after color compensation is activated. These signals are usually very small and are just slightly above background noise. To eliminate these artifacts, slowly reduce (by stepwise titration) the final concentration of Hybridization Probes in the color compensation run to the concentration used in the experimental assay (e.g., 0.2 µm each). Alternatively, use the non-automated analysis method (Fit Points) to manually raise the noise band. The multicolor compensation protocol is identical to that of the LightCycler Color Set. If you are an expert user, you may copy the macro from the Demo Macro folder (see 2.1.2), save it under a new name, and then change the settings in the Sample List (under Capillary View). Important: If a multicolor compensation object is to work properly, you must edit the analysisspecific sample list in the Color module. Most importantly, you must define the dominant channels (Figure 4). Figure 4: Using and editing a macro for multicolor color compensation Before starting the run, place the reagent capillaries into the carousel in the order listed above. Always place the capillary with buffer in the first position on the carousel. When the experiment is finished, select Color from the Analysis menu and then Save the CC Object (as in section 2.1.2). 9

10 2.2.2 Multicolor Hybridization Probe Applications continued Applying Color to a Multicolor Hybridization Probe Experiment You can now apply the parameter-specific color compensation object to the corresponding multicolor experiments. To activate the color compensation, click on Color in either the Run or the Analysis module, and choose the appropriate object under Select Color. Note: Select the appropriate channels for multicolor compensation, then click OK. Important: This color compensation will be valid for all experiments performed with the identical parameters. However, if you change the sequences of the hybridization probes, you will need to generate a new color compensation object. Even if you order a new batch of identical probes, it may be wise to re-run the color compensation experiment and compare the results obtained with the freshly generated ccc object to those obtained with the old ccc object. Automated Identification of Specific Color Objects by Using the "Color Comp ID Number The LightCycler SW version 4.0 and higher provides the option for an automated selection of a specific color compensation object. In the Capillary View under Samples there are several boxes offering room for additional run and/or sample information. The box for the color compensation identnumber (Color Comp ID) can be used to link an analysis file to the respective parameter-specific color compensation object. Especially when working with Macros, this ID number is highly convenient for the correct identification of color compensation objects. Example: Using the Demo Macro Color Set (Cat No ) for a dual color calibration run (LC Red 640 and 705), this Macro automatically labels the generated ccc.object with the Color Comp ID Any dual color run using this ccc.object can be automatically guided to this ccc.object by entering the identical number into the Color Comp ID box. The same function is available for customized applications. The ID box accepts any numbers, letters or letter-figure combinations. 10

11 3. Application Example Melting Curve Analysis without Color Figure 5: This example (from the LightCycler Multicolor Demo Set) shows a melting peak analysis in channel 670 of a target detected with the LightCycler Red 670 HybProbe set. Color was off, therefore no ccc object was applied to the results. Additional peaks seen in this channel arise from crosstalk generated by other probes that were labeled with LC Red 610, 640 and 705. These additional melting peaks prevent correct analysis of the data. Melting Curve Analysis with Color Figure 6: The identical data set from Figure 5 was analyzed in the Tm Calling module with Color on and the appropriate ccc object selected. The analysis of the target in channel 670 is now free of any interference from spectral overlap, and only the specific melting peak is visible. This data can now be accurately interpreted. Trademark: LightCycler is a trademark of a member of the Roche Group. The technology used for the LightCycler System is licensed from Idaho Technology Inc., Salt Lake City, UT, USA. SYBR is a registered trademark of Molecular Probes, Inc. 11

12 Roche Diagnostics GmbH Roche Applied Science Mannheim Germany

Fluorescent dyes for use with the

Fluorescent dyes for use with the Detection of Multiple Reporter Dyes in Real-time, On-line PCR Analysis with the LightCycler System Gregor Sagner, Cornelia Goldstein, and Rob van Miltenburg Roche Molecular Biochemicals, Penzberg, Germany

More information

Technical Note. Roche Applied Science. No. LC 18/2004. Assay Formats for Use in Real-Time PCR

Technical Note. Roche Applied Science. No. LC 18/2004. Assay Formats for Use in Real-Time PCR Roche Applied Science Technical Note No. LC 18/2004 Purpose of this Note Assay Formats for Use in Real-Time PCR The LightCycler Instrument uses several detection channels to monitor the amplification of

More information

COMPENSATION MIT Flow Cytometry Core Facility

COMPENSATION MIT Flow Cytometry Core Facility COMPENSATION MIT Flow Cytometry Core Facility Why do we need compensation? 1) Because the long emission spectrum tail of dyes causes overlap like with the fluorophores FITC and PE. 2) For sensitivity reasons,

More information

Optimizing Performance of the Transcreener ADP Assay for the BioTek Synergy 2 and 4 Multi-Mode Microplate Readers

Optimizing Performance of the Transcreener ADP Assay for the BioTek Synergy 2 and 4 Multi-Mode Microplate Readers Optimizing Performance of the Transcreener ADP Assay for the BioTek Synergy 2 and 4 Multi-Mode Microplate Readers Brad Larson 1, Karen Kleman-Leyer 1, Xavier Amouretti 2 1 BellBrook Labs, Madison, WI,

More information

Essentials of Real Time PCR. About Sequence Detection Chemistries

Essentials of Real Time PCR. About Sequence Detection Chemistries Essentials of Real Time PCR About Real-Time PCR Assays Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data is therefore collected

More information

RealStar HBV PCR Kit 1.0 11/2012

RealStar HBV PCR Kit 1.0 11/2012 RealStar HBV PCR Kit 1.0 11/2012 RealStar HBV PCR Kit 1.0 For research use only! (RUO) Product No.: 201003 96 rxns INS-201000-GB-02 Store at -25 C... -15 C November 2012 altona Diagnostics GmbH Mörkenstraße

More information

Real-Time PCR Vs. Traditional PCR

Real-Time PCR Vs. Traditional PCR Real-Time PCR Vs. Traditional PCR Description This tutorial will discuss the evolution of traditional PCR methods towards the use of Real-Time chemistry and instrumentation for accurate quantitation. Objectives

More information

2.500 Threshold. 2.000 1000e - 001. Threshold. Exponential phase. Cycle Number

2.500 Threshold. 2.000 1000e - 001. Threshold. Exponential phase. Cycle Number application note Real-Time PCR: Understanding C T Real-Time PCR: Understanding C T 4.500 3.500 1000e + 001 4.000 3.000 1000e + 000 3.500 2.500 Threshold 3.000 2.000 1000e - 001 Rn 2500 Rn 1500 Rn 2000

More information

Real-time PCR: Understanding C t

Real-time PCR: Understanding C t APPLICATION NOTE Real-Time PCR Real-time PCR: Understanding C t Real-time PCR, also called quantitative PCR or qpcr, can provide a simple and elegant method for determining the amount of a target sequence

More information

Application Note. Single Cell PCR Preparation

Application Note. Single Cell PCR Preparation Application Note Single Cell PCR Preparation From Automated Screening to the Molecular Analysis of Single Cells The AmpliGrid system is a highly sensitive tool for the analysis of single cells. In combination

More information

Real-time qpcr Assay Design Software www.qpcrdesign.com

Real-time qpcr Assay Design Software www.qpcrdesign.com Real-time qpcr Assay Design Software www.qpcrdesign.com Your Blueprint For Success Informational Guide 2199 South McDowell Blvd Petaluma, CA 94954-6904 USA 1.800.GENOME.1(436.6631) 1.415.883.8400 1.415.883.8488

More information

Stratagene QPCR Mouse Reference Total RNA

Stratagene QPCR Mouse Reference Total RNA Stratagene QPCR Mouse Reference Total RNA Instruction Manual Catalog #750600 Revision C.0 For Research Use Only. Not for use in diagnostic procedures. 750600-12 LIMITED PRODUCT WARRANTY This warranty limits

More information

Getting Started Guide

Getting Started Guide Primer Express Software Version 3.0 Getting Started Guide Before You Begin Designing Primers and Probes for Quantification Assays Designing Primers and Probes for Allelic Discrimination Assays Ordering

More information

QIAGEN Rotor Gene Q: Software V2.0

QIAGEN Rotor Gene Q: Software V2.0 QIAGEN Rotor Gene Q: Software V2.0 Instrument Setup Instructions for RT 2 Profiler PCR Arrays Preparation Before the Experiment (Presetting the Machine will save time for your run): Please make note of

More information

Software Manual - Part IIIa: MARS Data Analysis. Version 2.10

Software Manual - Part IIIa: MARS Data Analysis. Version 2.10 Software Manual - Part IIIa: MARS Data Analysis Version 2.10 OPTIMA Software Manual - Part IIIa: MARS Data Analysis BMG LABTECH This manual was designed to guide users through the software features. Although

More information

Highly specific and sensitive quantitation

Highly specific and sensitive quantitation PRODUCT ULLETIN SYR Select Master Mix SYR Select Master Mix Highly specific and sensitive quantitation SYR Select Master Mix offers advanced performance at an affordable price. SYR Select Master Mix is

More information

Plexor Systems Instrument Setup and Data Analysis for the Applied Biosystems 7300 and 7500 Real-Time PCR Systems

Plexor Systems Instrument Setup and Data Analysis for the Applied Biosystems 7300 and 7500 Real-Time PCR Systems Technical Manual Plexor Systems Instrument Setup and Data Analysis for the Applied Biosystems 7300 and 7500 Real-Time PCR Systems INSTRUCTIONS FOR USE OF PRODUCTS A4011, A4021, A4031, A4041, A4051 AND

More information

HBV Quantitative Real Time PCR Kit

HBV Quantitative Real Time PCR Kit Revision No.: ZJ0002 Issue Date: Aug 7 th, 2008 HBV Quantitative Real Time PCR Kit Cat. No.: HD-0002-01 For Use with LightCycler 1.0/LightCycler2.0/LightCycler480 (Roche) Real Time PCR Systems (Pls ignore

More information

QuantStudio 3D AnalysisSuite Software

QuantStudio 3D AnalysisSuite Software USER GUIDE QuantStudio 3D AnalysisSuite Software for use with QuantStudio 3D Digital PCR System Publication Number MAN0008161 Revision 1.0 For Research Use Only. Not for use in diagnostic procedures. For

More information

BD FACSComp Software Tutorial

BD FACSComp Software Tutorial BD FACSComp Software Tutorial This tutorial guides you through a BD FACSComp software lyse/no-wash assay setup run. If you are already familiar with previous versions of BD FACSComp software on Mac OS

More information

JBS FUNDAMENT Thermofluor Screen

JBS FUNDAMENT Thermofluor Screen Cat. No. CS-330 Amount 1 Kit For in vitro use only. Quality guaranteed for 3 months. Store at 4 C. Application Screen for thermal stability of proteins as a function of the FUNDAMENTAL variables ph and

More information

amplification tech Optical Design of CFX96 Real-Time PCR Detection System Eliminates the Requirement of a Passive Reference Dye

amplification tech Optical Design of CFX96 Real-Time PCR Detection System Eliminates the Requirement of a Passive Reference Dye amplification tech note 6047 Optical Design of CFX96 Real-Time PCR Detection System Eliminates the Requirement of a Passive Reference Dye Liz Jordan and Richard Kurtz, Gene Expression Division, io-rad

More information

Dye-Blob message: Example: Generally, this is due to incomplete excess dye removal of the cycle sequence reaction.

Dye-Blob message: Example: Generally, this is due to incomplete excess dye removal of the cycle sequence reaction. When sequence data is uploaded to ilab, an email is sent notifying the user that data is ready. The staff of the DNA facility has the ability to edit this message to include specific remarks about how

More information

ENVI Classic Tutorial: Atmospherically Correcting Multispectral Data Using FLAASH 2

ENVI Classic Tutorial: Atmospherically Correcting Multispectral Data Using FLAASH 2 ENVI Classic Tutorial: Atmospherically Correcting Multispectral Data Using FLAASH Atmospherically Correcting Multispectral Data Using FLAASH 2 Files Used in this Tutorial 2 Opening the Raw Landsat Image

More information

Determining the Quantity of Iron in a Vitamin Tablet. Evaluation copy

Determining the Quantity of Iron in a Vitamin Tablet. Evaluation copy Determining the Quantity of Iron in a Vitamin Tablet Computer 34 As biochemical research becomes more sophisticated, we are learning more about the role of metallic elements in the human body. For example,

More information

Immunophenotyping peripheral blood cells

Immunophenotyping peripheral blood cells IMMUNOPHENOTYPING Attune Accoustic Focusing Cytometer Immunophenotyping peripheral blood cells A no-lyse, no-wash, no cell loss method for immunophenotyping nucleated peripheral blood cells using the Attune

More information

Idaho Technology Food Security Systems. System Components. Idaho Technology. Food Security and Pathogen Detection

Idaho Technology Food Security Systems. System Components. Idaho Technology. Food Security and Pathogen Detection Incorporated in 1990, has years of experience developing exciting instruments, software at Inc. Part of s Incorporated in 1990, has years of experience developing exciting instruments, software at Inc.

More information

Qubit dsdna HS Assay Kits

Qubit dsdna HS Assay Kits Qubit dsdna HS Assay Kits For use with the Qubit 2.0 Fluorometer Table 1. Contents and storage information. Material Amount Concentration Storage Stability Qubit dsdna HS Reagent (Component A) 250 µl or

More information

INSTRUCTION Probemaker

INSTRUCTION Probemaker INSTRUCTION Probemaker Instructions for Duolink In Situ Probemaker PLUS (Art. no. 92009-0020) and Duolink In Situ Probemaker MINUS (Art. no. 92010-0020) Table of content 1. Introduction 4 2. Applications

More information

TaqMan Fast Advanced Master Mix. Protocol

TaqMan Fast Advanced Master Mix. Protocol TaqMan Fast Advanced Master Mix Protocol For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice. APPLIED

More information

Supporting Information. The G-triplex DNA could function as a new variety of DNA peroxidase

Supporting Information. The G-triplex DNA could function as a new variety of DNA peroxidase Supporting Information The G-triplex DNA could function as a new variety of DNA peroxidase Shaoru Wang +, Boshi Fu +, Shuang Peng, Xiaoe Zhang, Tian Tian*, Xiang Zhou* College of Chemistry and Molecular

More information

LEGENDplex Data Analysis Software

LEGENDplex Data Analysis Software LEGENDplex Data Analysis Software Version 7.0 User Guide Copyright 2013-2014 VigeneTech. All rights reserved. Contents Introduction... 1 Lesson 1 - The Workspace... 2 Lesson 2 Quantitative Wizard... 3

More information

Guide to using the Bio Rad CFX96 Real Time PCR Machine

Guide to using the Bio Rad CFX96 Real Time PCR Machine Guide to using the Bio Rad CFX96 Real Time PCR Machine Kyle Dobbs and Peter Hansen Table of Contents Overview..3 Setup Reaction Guidelines 4 Starting up the Software 5 Setup Protocol on Software 6 Setup

More information

Compass Software for Peggy User Guide

Compass Software for Peggy User Guide page 1 Compass Software for Peggy User Guide Copyright 2013 ProteinSimple. All rights reserved. ProteinSimple 3040 Oakmead Village Drive Santa Clara, CA 95051 Toll-free: (888) 607-9692 Tel: (408) 510-5500

More information

Introduction to Quantitative PCR

Introduction to Quantitative PCR Introduction to Quantitative PCR Methods and Applications Guide Introduction to Quantitative PCR Methods and Applications Guide IN 70200 D US and Canada Orders: 800-227-9770 x3 Technical Service: 800-227-9770

More information

Turning HTE Agilent LC/MS Data to Knowledge in 1hr

Turning HTE Agilent LC/MS Data to Knowledge in 1hr With permission Reaction Analytics Inc. 2011 Page 1 Turning HTE Agilent LC/MS Data to Knowledge in 1hr Neal Sach With permission Reaction Analytics Inc. 2011 Page 2 Contents Acquiring Data Fast demo method

More information

The Scheduled MRM Algorithm Enables Intelligent Use of Retention Time During Multiple Reaction Monitoring

The Scheduled MRM Algorithm Enables Intelligent Use of Retention Time During Multiple Reaction Monitoring The Scheduled MRM Algorithm Enables Intelligent Use of Retention Time During Multiple Reaction Monitoring Delivering up to 2500 MRM Transitions per LC Run Christie Hunter 1, Brigitte Simons 2 1 AB SCIEX,

More information

SmartFlare RNA Detection Probes: Principles, protocols and troubleshooting

SmartFlare RNA Detection Probes: Principles, protocols and troubleshooting Technical Guide SmartFlare RNA Detection Probes: Principles, protocols and troubleshooting Principles of SmartFlare technology RNA detection traditionally requires transfection, laborious sample prep,

More information

Scott Reierstad. Field Applications Scientist

Scott Reierstad. Field Applications Scientist Real-time PCR Training Scott Reierstad Field Applications Scientist Topics we ll cover Quick introduction to real-time PCR ABI-supported real-time chemistries Instrumentation Quantitation via Standard

More information

Quick Start Guide Chromeleon 7.2

Quick Start Guide Chromeleon 7.2 Chromeleon 7.2 7229.0004 Revision 1.0 July 2013 Table of Contents 1 Introduction... 1 1.1 About this Document... 1 1.2 Other Documentation... 2 2 Using Chromeleon... 3 2.1 Overview... 3 2.2 Starting Chromeleon...

More information

Eco TM 48 Real Time PCR System. Fastest block-based qpcr. Four colour multiplexing. Supports all chemistries. Fast cycling protocols

Eco TM 48 Real Time PCR System. Fastest block-based qpcr. Four colour multiplexing. Supports all chemistries. Fast cycling protocols A Bibby Scientific Company Eco TM 48 Real Time PCR System Fast cycling Fastest block-base HRM functionality Four colour multiplexing MIQE compliant Easy to use software Supports all chemistries Small footprint

More information

Quando si parla di PCR quantitativa si intende:

Quando si parla di PCR quantitativa si intende: Quando si parla di PCR quantitativa si intende: A. Una PCR che produce grandi quantità di DNA B. Una PCR che emette quanti di luce C. Una PCR che quantifica il numero di molecole stampo presenti all inizio

More information

BrainMaster tm System Type 2E Module & BMT Software for Windows tm. Helpful Hints

BrainMaster tm System Type 2E Module & BMT Software for Windows tm. Helpful Hints . BrainMaster tm System Type 2E Module & BMT Software for Windows tm Helpful Hints 1995-2004 BrainMaster Technologies, Inc., All Rights Reserved BrainMaster and From the Decade of the Brain are registered

More information

Supporting information. Molecular Encoder - Decoder Based on Assembly of Graphene with Dye-labeled DNA

Supporting information. Molecular Encoder - Decoder Based on Assembly of Graphene with Dye-labeled DNA Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2014 Supporting information Molecular Encoder - Decoder Based on Assembly of Graphene with Dye-labeled

More information

Path-ID Multiplex One-Step RT-PCR Kit

Path-ID Multiplex One-Step RT-PCR Kit USER GUIDE Path-ID Multiplex One-Step RT-PCR Kit TaqMan probe-based multiplex one-step real-time RT-PCR detection of RNA targets Catalog Numbers 4428206, 4428207, 4440022 Publication Part Number 4440907

More information

Experiment #5: Qualitative Absorption Spectroscopy

Experiment #5: Qualitative Absorption Spectroscopy Experiment #5: Qualitative Absorption Spectroscopy One of the most important areas in the field of analytical chemistry is that of spectroscopy. In general terms, spectroscopy deals with the interactions

More information

Methods and Application Guide. Introduction to Quantitative PCR

Methods and Application Guide. Introduction to Quantitative PCR Methods and Application Guide Introduction to Quantitative PCR Introduction to Quantitative PCR Methods and Application Guide Stratagene USA and Canada Order: 800-424-5444 x3 Technical Services: 800-894-1304

More information

Data Analysis on the ABI PRISM 7700 Sequence Detection System: Setting Baselines and Thresholds. Overview. Data Analysis Tutorial

Data Analysis on the ABI PRISM 7700 Sequence Detection System: Setting Baselines and Thresholds. Overview. Data Analysis Tutorial Data Analysis on the ABI PRISM 7700 Sequence Detection System: Setting Baselines and Thresholds Overview In order for accuracy and precision to be optimal, the assay must be properly evaluated and a few

More information

Absolute Quantification Getting Started Guide

Absolute Quantification Getting Started Guide 5 cdna Reverse Primer Oligo d(t) or random hexamer Synthesis of 1st cdna strand 3 5 cdna Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Absolute Quantification Getting Started Guide Introduction

More information

FluorChem Q. A Quantitative Western Blot Imaging Solution

FluorChem Q. A Quantitative Western Blot Imaging Solution FluorChem Q A Quantitative Western Blot Imaging Solution Contents FluorChem Q Innovative Design The FluorChem Q is a total solution for quantitative fluorescent and chemiluminescent Western blot imaging

More information

MS Access Lab 2. Topic: Tables

MS Access Lab 2. Topic: Tables MS Access Lab 2 Topic: Tables Summary Introduction: Tables, Start to build a new database Creating Tables: Datasheet View, Design View Working with Data: Sorting, Filtering Help on Tables Introduction

More information

Manual Analysis Software AFD 1201

Manual Analysis Software AFD 1201 AFD 1200 - AcoustiTube Manual Analysis Software AFD 1201 Measurement of Transmission loss acc. to Song and Bolton 1 Table of Contents Introduction - Analysis Software AFD 1201... 3 AFD 1200 - AcoustiTube

More information

PATHOGEN DETECTION SYSTEMS BY REAL TIME PCR. Results Interpretation Guide

PATHOGEN DETECTION SYSTEMS BY REAL TIME PCR. Results Interpretation Guide PATHOGEN DETECTION SYSTEMS BY REAL TIME PCR Results Interpretation Guide Pathogen Detection Systems by Real Time PCR Microbial offers real time PCR based systems for the detection of pathogenic bacteria

More information

ArC Amine Reactive Compensation Bead Kit

ArC Amine Reactive Compensation Bead Kit ArC Amine Reactive Compensation Bead Kit Catalog no. A1346 Table 1. Contents and storage information. Material Amount Composition Storage Stability ArC reactive beads (Component A) ArC negative beads (Component

More information

Amino Acid Analyzer L-8900

Amino Acid Analyzer L-8900 Amino Acid Analyzer L-8900 VWR - Hitachi Your Partner in Amino Acid Analysis Hitachi has manufactured more than 1800 Amino Acid Analysers for over 40 years. The new Amino Acid Analyser Model L-8900 is

More information

Genolution Pharmaceuticals, Inc. Life Science and Molecular Diagnostic Products

Genolution Pharmaceuticals, Inc. Life Science and Molecular Diagnostic Products Genolution Pharmaceuticals, Inc. Revolution through genes, And Solution through genes. Life Science and Molecular Diagnostic Products www.genolution1.com TEL; 02-3010-8670, 8672 Geno-Serum Hepatitis B

More information

Application Note. Biotechnology Explorer Crime Scene Investigator PCR Basics. Kit: A Real-Time PCR Extension

Application Note. Biotechnology Explorer Crime Scene Investigator PCR Basics. Kit: A Real-Time PCR Extension Biotechnology Explorer Crime Scene Investigator PCR Basics Kit: Table of Contents Introduction.............................................. 2 Learning Objectives......................................

More information

P i Per Pyrophosphate Assay Kit

P i Per Pyrophosphate Assay Kit P i Per Pyrophosphate Assay Kit Table 1. Contents and storage information. Material Amount Concentration Storage Stability Amplex Red reagent (Component A, MW = 257) 5 vials, 0.26 mg each DMSO, anhydrous

More information

Front face fluorescence analysis to monitor food process contaminants

Front face fluorescence analysis to monitor food process contaminants Front face fluorescence analysis to monitor food process contaminants Fluoralys technology Fluorescence is a natural phenomenon of light emission when a molecule has received light energy, with 10% of

More information

Products - Microarray Scanners - SpotLight Two-Color Microarray Fluorescence Scanners New! SpotLight 2 now available!

Products - Microarray Scanners - SpotLight Two-Color Microarray Fluorescence Scanners New! SpotLight 2 now available! Products - Microarray Scanners - SpotLight Two-Color Microarray Fluorescence Scanners New! SpotLight 2 now available! Arrayit SpotLight Two-Color Microarray Scanners provide the market s most affordable

More information

Universidade Estadual de Maringá

Universidade Estadual de Maringá Universidade Estadual de Maringá Disciplina: Biologia Molecular Sequenciamento de ácidos nucléicos Profa. Dra. Maria Aparecida Fernandez Maxan e Gilbert - quebra química Berg, Gilbert and Sanger dideoxinucleotideos

More information

Roche Applied Science Technical Note No. LC 13/2001

Roche Applied Science Technical Note No. LC 13/2001 Roche Applied Science Technical Note No. LC 13/2001 LightCycler Relative Quantification Purpose of this Note The LightCycler provides great flexibility especially to the user interested in quantitative

More information

Application Sheet for Rivaroxaban (Xarelto ) Standard Range with. BIOPHEN Heparin LRT (#221011/221013) RUO

Application Sheet for Rivaroxaban (Xarelto ) Standard Range with. BIOPHEN Heparin LRT (#221011/221013) RUO Instrument Adaptation The attached instrument adaptation has been prepared and validated by the reagent manufacturer, Hyphen-Biomed. Instrument Siemens BCS-XP Product BIOPHEN Heparin LRT Analyte Rivaroxaban

More information

TruSeq Custom Amplicon v1.5

TruSeq Custom Amplicon v1.5 Data Sheet: Targeted Resequencing TruSeq Custom Amplicon v1.5 A new and improved amplicon sequencing solution for interrogating custom regions of interest. Highlights Figure 1: TruSeq Custom Amplicon Workflow

More information

Spin-Lattice Relaxation Times

Spin-Lattice Relaxation Times Spin-Lattice Relaxation Times Reading Assignment: T. D. W. Claridge, High Resolution NMR Techniques in Organic Chemistry, Chapter 2; E. Breitmaier, W. Voelter, Carbon 13 NMR Spectroscopy,3rd Ed., 3.3.2.

More information

Device configurator DRC200

Device configurator DRC200 Operating manual 42/49-27 EN Engineer IT Device configurator DRC200 R&C Process Data Management Software Impressum Device configurator DRC200 Operating manual Document No. 42/49-27 EN Date of issue: 11.02

More information

Instructions for Use. CyAn ADP. High-speed Analyzer. Summit 4.3. 0000050G June 2008. Beckman Coulter, Inc. 4300 N. Harbor Blvd. Fullerton, CA 92835

Instructions for Use. CyAn ADP. High-speed Analyzer. Summit 4.3. 0000050G June 2008. Beckman Coulter, Inc. 4300 N. Harbor Blvd. Fullerton, CA 92835 Instructions for Use CyAn ADP High-speed Analyzer Summit 4.3 0000050G June 2008 Beckman Coulter, Inc. 4300 N. Harbor Blvd. Fullerton, CA 92835 Overview Summit software is a Windows based application that

More information

Reagent Guide. Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems

Reagent Guide. Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Reagent Guide Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems Reagent Guide Copyright 2008, 2010 Applied Biosystems. All

More information

Recognition Kinetics of Biomolecules at the Surface of Different-Sized Spheres

Recognition Kinetics of Biomolecules at the Surface of Different-Sized Spheres Supporting Material Recognition Kinetics of Biomolecules at the Surface of Different-Sized Spheres Jun Hu, Cong-Ying Wen, Zhi-Ling Zhang, Min Xie, Hai-Yan Xie, and Dai-Wen Pang * Key Laboratory of Analytical

More information

qpcr Technical Guide Detection Methods Primer and Probe Design Instrumentation Applications Guide

qpcr Technical Guide Detection Methods Primer and Probe Design Instrumentation Applications Guide qpcr Technical Guide Detection Methods Primer and Probe Design Instrumentation Applications Guide Table of Contents qpcr Technical Guide Introduction... 1 Quantitative PCR: How does it work?... 2 qpcr

More information

MassHunter for Agilent GC/MS & GC/MS/MS

MassHunter for Agilent GC/MS & GC/MS/MS MassHunter for Agilent GC/MS & GC/MS/MS Next Generation Data Analysis Software Presented by : Terry Harper GC/MS Product Specialist 1 Outline of Topics Topic 1: Introduction to MassHunter Topic 2: Data

More information

Creating Fill-able Forms using Acrobat 8.0: Part 1

Creating Fill-able Forms using Acrobat 8.0: Part 1 Creating Fill-able Forms using Acrobat 8.0: Part 1 The first step in creating a fill-able form in Adobe Acrobat is to generate the form with all its formatting in a program such as Microsoft Word. Then

More information

Your Personal Pipetting Robot

Your Personal Pipetting Robot Your Personal Pipetting Robot Quality creates value www.dornier-ltf.com Easiness meets......versatility The PIRO Liquid Handling System provides a versatile pipetting platform with many options for the

More information

RealLine HCV PCR Qualitative - Uni-Format

RealLine HCV PCR Qualitative - Uni-Format Instructions for use PCR KIT FOR EXTRACTION OF RNA AND REAL TIME PCR DETECTION KIT FOR HEPATITIS C VIRUS RNA Research Use Only Qualitative Uni Format VBD0798 48 tests valid from: December 2013 Rev11122013

More information

TOPSPIN INSTRUCTIONS

TOPSPIN INSTRUCTIONS TOPSPIN INSTRUCTIONS 1. How to start TOPSPIN? 2. The TOPSPIN window 3. How to open an old dataset? 4. How to create a new dataset? 5. How to lock and shim? 6. How to acquire FID signal and modify acquisition

More information

Copyright 2007 Casa Software Ltd. www.casaxps.com. ToF Mass Calibration

Copyright 2007 Casa Software Ltd. www.casaxps.com. ToF Mass Calibration ToF Mass Calibration Essentially, the relationship between the mass m of an ion and the time taken for the ion of a given charge to travel a fixed distance is quadratic in the flight time t. For an ideal

More information

IncuCyte ZOOM Whole Well Imaging Overview

IncuCyte ZOOM Whole Well Imaging Overview IncuCyte ZOOM Whole Well Imaging Overview With the release of the 2013B software, IncuCyte ZOOM users will have the added ability to image the complete surface of select multi-well plates and 35 mm dishes

More information

Waters Corporation. Waters 2690/5 USER & TROUBLESHOOTING GUIDE

Waters Corporation. Waters 2690/5 USER & TROUBLESHOOTING GUIDE Waters Corporation Waters 2690/5 USER & TROUBLESHOOTING GUIDE Contents 2690/5 Theory Setup procedures. Troubleshooting the 2690/5 User maintenance of the 2690/5 Spare Parts 2 2690/5 Theory 2690/5 Solvent

More information

MICROSOFT OFFICE ACCESS 2007 - NEW FEATURES

MICROSOFT OFFICE ACCESS 2007 - NEW FEATURES MICROSOFT OFFICE 2007 MICROSOFT OFFICE ACCESS 2007 - NEW FEATURES Exploring Access Creating and Working with Tables Finding and Filtering Data Working with Queries and Recordsets Working with Forms Working

More information

SpecLine Offline Spectroscopy Analysis Software SPECLINE-P/SPECLINE-U. Installation and Operation Manual Document Number 000-20000-090-02-1209

SpecLine Offline Spectroscopy Analysis Software SPECLINE-P/SPECLINE-U. Installation and Operation Manual Document Number 000-20000-090-02-1209 SpecLine Offline Spectroscopy Analysis Software SPECLINE-P/SPECLINE-U Installation and Operation Manual Document Number 000-20000-090-02-1209 Offices: Ocean Optics, Inc. World Headquarters 830 Douglas

More information

Tuning & Mass Calibration

Tuning & Mass Calibration Tuning & Mass Calibration 1 1 The Sample List Sample List Name Project Name 2 The sample list is the top level screen in the TurboMass Gold Software. Data storage is set up in PROJECT files and within

More information

Meso Scale Discovery. WINDOWS is a registered trademark of Microsoft Corporation

Meso Scale Discovery. WINDOWS is a registered trademark of Microsoft Corporation Meso Scale Discovery M S D M S D Meso Scale Discovery, a division of Meso Scale Diagnostics, LLC. (MSD) 9238 Gaither Rd, Gaithersburg, MD 20877 Phone: 240.631.2522 Fax: 240.632.2219 email: sales@meso-scale.com

More information

ENVI Classic Tutorial: Atmospherically Correcting Hyperspectral Data using FLAASH 2

ENVI Classic Tutorial: Atmospherically Correcting Hyperspectral Data using FLAASH 2 ENVI Classic Tutorial: Atmospherically Correcting Hyperspectral Data Using FLAASH Atmospherically Correcting Hyperspectral Data using FLAASH 2 Files Used in This Tutorial 2 Opening the Uncorrected AVIRIS

More information

White Paper. Zecotek Visible Fiber Laser Platform. Enabling the future of laser technology

White Paper. Zecotek Visible Fiber Laser Platform. Enabling the future of laser technology White Paper Zecotek Visible Fiber Laser Platform Enabling the future of laser technology Zecotek Photonics Inc. (TSX- V: ZMS; Frankfurt: W1I) www.zecotek.com is a Canadian photonics technology company

More information

MiSeq: Imaging and Base Calling

MiSeq: Imaging and Base Calling MiSeq: Imaging and Page Welcome Navigation Presenter Introduction MiSeq Sequencing Workflow Narration Welcome to MiSeq: Imaging and. This course takes 35 minutes to complete. Click Next to continue. Please

More information

Designing Assays for the TaqMan Protein Assay

Designing Assays for the TaqMan Protein Assay Designing Assays for the TaqMan Protein Assay Antibody Selection...2 Assay Design...2 Antibody Search...3 Biotinylated Antibodies...3 Working with pre-biotinylated antibodies...4 Working with non-biotinylated

More information

Appendix C. Vernier Tutorial

Appendix C. Vernier Tutorial C-1. Vernier Tutorial Introduction: In this lab course, you will collect, analyze and interpret data. The purpose of this tutorial is to teach you how to use the Vernier System to collect and transfer

More information

ab102884 FITC Conjugation Kit Protocol

ab102884 FITC Conjugation Kit Protocol ab102884 FITC Conjugation Kit Protocol Antibody and protein modification This product is for research use only and is not intended for diagnostic use. Version 1 Last Updated 4 November 2013 Table of Contents

More information

CFSE Cell Division Assay Kit

CFSE Cell Division Assay Kit CFSE Cell Division Assay Kit Item No. 10009853 Customer Service 800.364.9897 * Technical Support 888.526.5351 www.caymanchem.com TABLE OF CONTENTS GENERAL INFORMATION 3 Materials Supplied 4 Precautions

More information

AxyPrep TM Mag PCR Clean-up Protocol

AxyPrep TM Mag PCR Clean-up Protocol AxyPrep TM Mag PCR Clean-up Protocol Intro The AxyPrep Mag PCR Clean-up kit utilizes a unique paramagnetic bead technology for rapid, high-throughput purification of PCR amplicons. Using this kit, PCR

More information

PDA Software Getting Started Guide

PDA Software Getting Started Guide PDA Software Getting Started Guide 34 Maple Street Milford, MA 01757 WAT053020TP, Revision 1 NOTICE The information in this document is subject to change without notice and should not be construed as a

More information

Beginner s Guide to Real-Time PCR

Beginner s Guide to Real-Time PCR Beginner s Guide to Real-Time PCR 02 Real-time PCR basic principles PCR or the Polymerase Chain Reaction has become the cornerstone of modern molecular biology the world over. Real-time PCR is an advanced

More information

Data Management Software CL-S10w Ver.1.3. Instruction Manual

Data Management Software CL-S10w Ver.1.3. Instruction Manual Data Management Software CL-S10w Ver.1.3 Instruction Manual Safety Warning Please read this manual and the instrument and computer manual carefully beforehand and use the software properly and safely.

More information

flexanalysis User Manual

flexanalysis User Manual flexanalysis User Manual Version 3.0 (September 2006) Copyright Copyright 2006 Bruker Daltonik GmbH This document is published by Bruker Daltonik Bremen. All rights reserved. Reproduction, adaptation or

More information

Technical Bulletin. An Introduction to Compensation for Multicolor Assays on Digital Flow Cytometers

Technical Bulletin. An Introduction to Compensation for Multicolor Assays on Digital Flow Cytometers An Introduction to Compensation for Multicolor Assays on Digital Flow Cytometers BD Biosciences, San Jose, CA Contents 2 Introduction to compensation 5 Tips for setting up compensation 10 Tools for easy,

More information

Corona process The new ZEISS spectrometer system for the food industry

Corona process The new ZEISS spectrometer system for the food industry Interactive PDF Version 1.0 Start Trust needs reliability quality needs ZEISS The real challenge is to offer a product with consistent quality using raw materials which are themselves subject to ongoing

More information

TotalChrom Workstation User s Guide - Volume II

TotalChrom Workstation User s Guide - Volume II TotalChrom Workstation User s Guide - Volume II Release History Part Number Release Publication Date N515-6024 B December 2004 User Assistance PerkinElmer 710 Bridgeport Avenue Shelton, CT 06484-4794 Or

More information

Hepatitis B Virus. genesig Advanced Kit. DNA testing. Everything... Everyone... Everywhere... Core Protein Region. 150 tests.

Hepatitis B Virus. genesig Advanced Kit. DNA testing. Everything... Everyone... Everywhere... Core Protein Region. 150 tests. TM Primerdesign Ltd TM Primerdesign Ltd Hepatitis B Virus Core Protein Region genesig Advanced Kit 150 tests DNA testing Everything... Everyone... Everywhere... For general laboratory and research use

More information

User Guide for the 21 CFR Part 11 Module in SDS Software v1.4

User Guide for the 21 CFR Part 11 Module in SDS Software v1.4 Applied Biosystems 7500/7500 Fast Real-Time PCR System User Guide for the 21 CFR Part 11 Module in SDS Software v1.4 Introduction Installation Logging in to the SDS Software Configuring the 21CFR11 Module

More information

Application Note # LCMS-81 Introducing New Proteomics Acquisiton Strategies with the compact Towards the Universal Proteomics Acquisition Method

Application Note # LCMS-81 Introducing New Proteomics Acquisiton Strategies with the compact Towards the Universal Proteomics Acquisition Method Application Note # LCMS-81 Introducing New Proteomics Acquisiton Strategies with the compact Towards the Universal Proteomics Acquisition Method Introduction During the last decade, the complexity of samples

More information