PETIKAN KEPUTUSAN JAWATANKUASA TETAP KEWANGAN BIL.3 (2014) PADA 25 SEPTEMBER 2014

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Bil Jenis Perkhidmatan Jenis Analisis/ Catatan Unit UPM Institusi 1 High pressure homogenizer Preparation of nanoemulsion Hour 110.00 121.00 133.00 2 Zeta sizer Measurement of partical size and zeta potential Sample 110.00 121.00 133.00 3 Chemistry analyzer Biochemistry analysis (Lipid profile, toxicity test) Sample 7.00 8.00 9.00 4 Freeze dryer (pilot scale) Freeze dryer hour 27.00 29.00 32.00 5 DNA sequencer Multiplex gene expression analysis row (8 wells) 220.00 242.00 266.00 6 GenomeLab genetic analysis system (GeXP) Multiplex gene expression analysis row (8 wells) 220.00 242.00 266.00 7 Flow cytometer Flow cytometric analyses (Including sheath fluid, cleaning solution) Flow cytometric analyses (Excluding sheath fluid, cleaning solution) hour 175.00 193.00 212.00 hour 117 129.00 142.00 8 Densitometer Gel scanning gel 22.00 25.00 27.00 9 Flourescence miscroscope Slide observation slide 22.00 25.00 27.00 1

Bil Jenis Perkhidmatan Jenis Analisis/ Catatan Unit UPM Institusi 10 Multimode microplate reader Absorbance, fluorescence and luminescence readings 1. plate for endpoint reading 2. hour for kinetic reading 1.22.00 2.61.00 1.25.00 2.67.00 11 PCR thermal cycler Amplification of PCR products hour 33.00 36.00 40.00 1. 27.00 2. 73.00 12 Real-Time PCR (RT-PCR) Gene expression analysis hour 99.00 109.00 120.00 13 Nanodrop Measurement of RNA concentration and purity sample 22.00 24.00 26.00 14 Transblotter Western blot gel 22.00 25.00 27.00 15 GCMS-QQQ GCMS analysis (full screening and SRM) sample 321.00 353.00 388.00 16 Solvent extraction 1. Alcohol (methanol, ethanol, butanol etc.) 2. Water 3. Other (Acetone, Chloroform, DCM, Petroleum ether, hexane, etc.) 4. Validated phenolic extraction (ISO 17025 accredited) sample 319.00 351.00 387.00 2

Bil Jenis Perkhidmatan Jenis Analisis/ Catatan Unit UPM Institusi 17 Supercritical fluid extraction Supercritical carbon dioxide fluid extraction (1 L capacity) hour 244.00 269.00 296.00 18 Near critical fluid extraction (subcritical water extraction system) Near critical/ subcritical water extraction (500 ml capacity) 19 Phenolic content determination Total phenolic content (standard: gallic acid) 20 Phenolic content determination Total flavonoid content (standard: rutin) 21 In-vitro antioxidant activity test DPPH Scavenging activity (ISO 17025 accredited) hour 238.00 262.00 288.00 sample 362.00 398.00 438.00 sample 186.00 205.00 225.00 sample 391.00 430.00 473.00 22 In-vitro antioxidant activity test ABTS cation radical scavenging activity sample 353.00 389.00 428.00 23 In-vitro antioxidant activity test Ferric reducing power sample 361.00 397.00 436.00 24 In-vitro antioxidant activity test β-carotene bleaching activity sample 431.00 474.00 521.00 25 In-vitro antioxidant activity test Gavinoxyl radical scavenging activity (ESR) sample 334.00 367.00 404.00 26 In-vitro antioxidant activity test Iron chelating activity sample 235.00 258.00 284.00 3

Bil Jenis Perkhidmatan Jenis Analisis/ Catatan Unit UPM Institusi 27 In-vitro antioxidant activity test Hydroxyl radical scavenging activity (ESR) sample 466.00 512.00 564.00 28 In-vitro antioxidant activity test Electron spin resonance spectrometer measurement (ESR) sample 74.00 81.00 89.00 29 Proximate analysis Fat, protein, carbohydrate, ash and moisture sample 220.00 242.00 266.00 30 Development & characterization of nanoemulsion 1. Preparation of nanoemulsion by high pressure homogenizer 2. Characterization of nanoemulsion by zeta sizer 31 Cell culture Provide training/analysis on the cell culture techniques/selected assays sample 456.00 504.00 555.00 training package 825.00 908.00 999.00 32 Cell cycle Analysis of cell cycle by flow cytometer sample 120.00 132.00 145.00 33 Annexin V-FITC Analysis of apoptosis (Annexin V-FITC method) by flow cytometer 34 Mitochondrial membrane potential (MMP) Analysis of mitochondrial membrane potential (MMP) by flow cytometer sample 127.00 140.00 154.00 sample 116.00 128.00 141.00 35 Reactive oxygen species Analysis of reactive oxygen species by flow cytometer sample 117.00 129.00 142.00 4

Bil Jenis Perkhidmatan Jenis Analisis/ Catatan Unit UPM Institusi 36 Antioxidant genes Analysis of multiplex gene expression on 5 of antioxidant genes by GeXP sample 658.00 724.00 796.00 37 Apoptosis genes Analysis of multiplex gene expression on 10 of apoptosis genes by GeXP 38 Cardiovascular disease (CVD) genes Analysis of multiplex gene expression on 10 of CVD genes (cholesterol metabolism) by GeXP 39 Diabetic genes Analysis of multiplex gene expression on 10 of diabetic genes by GeXP 40 Anti-aging genes Analysis of multiplex gene expression on 10 of anti-aging genes related to Alzheimer's disease by GeXP 41 Halal verification genes Analysis of multiplex gene expression on 5 of Halal-related genes by GeXP sample 658.00 724.00 796.00 42 RNA extraction RNA extraction sample 44.00 48.00 53.00 5