Tentative program on line March, 2015. www.icmss2015.com. Convention Center Hotel Patagónico Puerto Varas Chile



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Tentative program on line March, 2015 www.icmss2015.com Convention Center Hotel Patagónico Puerto Varas Chile Sunday, March 15 Registration Registration Convention Center Hotel Patagónico, Puerto Varas, Chile Poster set up full conference Stand set up full conference Buses for Operture : 18 h Hotel Patagónico 15:00-18:00 Operture & Dinner : Teatro del Lago Frutillar Región de Los Lagos. Welcome ICMSS 2015: Viviana Cachicas ISP Chile Welcome by Regional & National Chilean Authorities Welcome main Sponsors & Reference Organizations Remembering ICMSS 2013 Anthony Zammit, Australia Special Award for participating in the conference since 1994 19:00-23:00

Magistral Conference Monday, March 16 Climate change: What about Latin America influence. Marcelo Mena PhD. Ex- Director Center for Sustainability Research at Universidad Andres Bello Chile & Under Minister of the Environment of Chile 09:00-10:00 CONFERENCE 1 Location: Salón Tronador Break 10:00-10:30 Conferences : Shellfish and Environment Chair: Anthony Zammit Location: Salón Tronador 10:30-11:30 Growth of Bacteria in Shellfish and the emergence of seafood related outbreaks. Romilio Espejo. Omics-Chile CONFERENCE 2 ISA virus in Chile: Coexisting Shellfish and Salmon farms. Marco Godoy CIBA-ETECMA Chile. 11.30-12:30 Lunch 12.30-13:45 h Parallel session N 1 : Oral Sessions Simultaneous Translation Biotoxin & Phytoplankton Session: Management and surveillance Salón Volcan A Day Chair: Claudia Rozas I- HAB monitoring Virus Virus & Health Salón Volcán B Day chair/coordinador : Carlos Aranda 14:00-18:00 Break 16:00:16:30

1. Leonardo Guzmán, IFOP, Chile : Something is happening in fjords and channels of Southern Chile (41-55 ): the case of Alexandrium catenella and Paralytic Shellfish Poison for the past 40 years. (14:00-14:30) 2. Brian Roughan, New Zealand: Mussel long-line culture and the risks of toxic Dinophysis in the water column. (14:30-15:00) 3. Joe Silke, Ireland: Operational Forecasting of Harmful Algal Blooms and Toxicity in Ireland. (15:00-15:30) 4. Jorge Mardones, IMAS, University of Tasmania: New insights on the ecophysiology of Alexandrium catenella from Chilean fjords. (15:30-16:00) BREAK (16:00 to 16:30) II- Biotoxin monitoring: 5. Mario Castillo, COFEPRIS, México: The Red Tide Project as a health monitoring system for the shellfish 1.- Miguel O Ryan, Universidad de Chile, Chile: Impact of NoV infection : An overview including Chilean data. (14:00-14:30) 2.- Viviana Cachicas, ISP, Chile: A characterization of outbreaks associated with NoV in Chile. (14:30-15:00) 3.Jacqueline Wood USFDA/DSST/Dauphin Island, AL Extraction, Detection and Quantification of Norovirus in Shellfish Associated with Illnesses (15:00-15:30) 4.- David Kingsley, Ag Res Service, Virologist, USDA, USA: Investigation of Norovirus Viability. (15:30-16:00) BREAK (16:00 to 16:30) 5- Kevin Hunt, Shellfish Microbiology Unit, Marine Institute. Ireland: Norovirus detection in oysters: the effect of sample size on the precision of production site virus concentration estimates.

exposed to Harmful Algal Blooms (HABs) (16:30-16:55) 6. Mathias Schramm, IFSC, Brazil: Occurrence of phycotoxins in the coast of Santa Catarina, the largest aquaculture mollusks producer in Brazil. (16:55-17:15) 7. Ronel Biré, ANSES, France: Review of three years of monitoring of palytoxin-group toxins in different marine organisms in Villefranche bay on the French Mediterranean coast. (17:15-17:35) (16:30-17:00) 6.- Kenneth Moore, ISSC US (TBC), USA: Male coli phage relevance for Interstate Shellfish Sanitation (17:00-17:30) 7.- Thomas Howel, Steve Jones, Results of three Field Studies presented at the MSC information Meeting: Demostrating Potential Utility of Male-Specific Coliphage in the NSSP. (17:30-18:00) 8. Grace Mellano, Canada: Communicating with stakeholders: new tools to meet challenges (17:35-18:00) Workshop n 1 Fluorometry of High Repetition FRRF= Applied to photosynthetic cells of dinoflagellates/fluorometria de altas tasas de repetición FRRF3 aplicado a células fotosintéticas de dinoflagelados. Laboratorio Plancton Andino. Puerto Varas. Chile. Coordinador : Monica Jara ISP Chile 18:15-20:30h

CONFERENCE 4 Tuesday, March 17 Genomic of shellfish Chair: Enrico Buenaventura Location: Salón Tronador Academic collaboration for traceability to shellfish producers. Marcela Astorga PhD. University Austral de Valdivia Chile 9:00: 9 30 CONFERENCE 5 CONFERENCE 6 Molecular traceability in Chilean blue mussel. Genomic tools to enforce food quality and safety. M Angélica Larrain PhD Universidad de Chile. 9:30-10:00 Metagenomics for pathogen detection in Shellfish. Romilio Espejo. Omics- Chile 10:00-10:30 Break & Posters 10:30-11:30 Parallel session N 2: Oral presentation. Simultaneous translation. Biotoxin & Phytoplankton Location: Salón Volcan A Day Chair: Leonardo Guzman Virus Location: Salón Volcán B Day Chair/Coordinador : Michelle Price Hayward 11:30-18:00 Lunch 12 :30 a 13:45

I- Analytical methods: perspectives and challenges 9.- Begoña Ben-Gigirey, EURLMB, Spain: The transition from mouse bioassay to chemical methods for the determination of saxitoxin group toxins: the EURLMB experience. (11:30-12:00) 10.-Tim Harwood, Cawthron Institute, New Zealand: Single laboratory validation of a UPLC-HILIC-MS/MS method for quantitation of paralytic shellfish toxins in twelve commercially produced bivalve shellfish species (12:00-12:30) LUNCH (12:30 to 13:45) 11. Arjen Gerssen, Rikilt, Holland: Screening of a wide variety of phycotoxins in different matrices using liquid chromatography high resolution mass spectrometry (14:00-14:30) 12.- Beatriz San Martin, Universidad de Chile : 8. Rodrigo Gonzalez, Universidad de Concepción Chile. Detección of the virus of Hepatitis A (HAV) in marine organims and other environmental matrices. (11:30-12:00) II- Virus Management 9.- Mario Latini, Centro di Referenza Nazionale Ancona, Italy : Evaluation of the characteristics of the wastewater treatment plants for the microbial contamination of bivalve molluscs production areas in the Italian mid-adriatic coast (12:00-12:30) LUNCH (12:30 to 13:45) 11- Bill Dore, Shellfish Safety Marine Institute, Ireland: Assessing the impact of risk management procedures on norovirus concentrations in oysters at a production site over a three year period. (14:00-14:30) Afternoon Break 16: 00-16:30

MARINE LIPOPHILIC TOXINS; From the bioassay to LC-MS / MS (14:30-15:00) II- Alternative technologies for biotoxins detection 13.- David Cassis, Jellet, Chile: Volunteer-based harmful algae monitoring networks with rapid toxin detection as an added safety layer and managing option for aquaculture. (15:00-15:30) 14.- Marcia Bodero, Rikilt, Holland: Mode of action-based bioassays for marine biotoxins causing gastrointestinal disturbances (15:30-16:00) 12.-.Michèle Gourmelon & Soizick Le Guyader, 1LSEM-SG2M-RBE, IIFREMER, Université de Caen, France: Occurrence of bacterial and viral enteric pathogens and marine bacteria and discrimination of faecal sources in shellfish-harvesting areas and their catchments in France (14:30-15:00) 13.- Michelle Price Hayward, Cefas, UK: Risk Assessment and Risk mapping. Getting the most out of sanitary surveys. (15:00-15:30) BREAK (16:00 to 16:30) BREAK (16:00 to 16:30) 15.- Karrie Melville, Neogen, UK: Development and validation of a range of lateral flow immunoassays for the rapid screening of marine biotoxins (ASP, DSP, PSP) in shellfish (16:30-16:55) 16.- Bovee, Toine / Rikilt, Holland:

High throughput assays for sensitive screening of marine neurotoxins in seafood (16:55-17:15) 17.- Bovee, Toine Rikilt Wageningen, Holland: Cell based bioassays for the detection of marine toxins in fish and shellfish for replacing animal testing. (17:15-17:35) Workshop N 2: Seremi Laboratory Detection kit for Biotoxins. David Cassis Jelllet Co. Chair : Cristina Hernandez Location: Laboratorio Seremi Región de Los Lagos 18:00-20:00 Workshop N 3 Dye tracing studies in shellfarm areas, Gregory Goblick US FDA Shellfish and Aquaculture Policy Branch. Teorical approach Cochair : Carlos Aranda Universidad de Los Lagos Location: Salón Tronador 18:00-20:00 Technologic tour N 1: Shellfish and Industry Farm. MEJILLONES AMERICA SA PLANTA DE PROCESO. Chair: Gonzalo Diaz, Food Inspector Seremi Salud Región de Los Lagos Chile & Monica Jara ISP-Chile 16:30-21:00

Wesneyday, March 18 Round Table: International practices in Bivalves sanitation programme Location: Salón Tronador Chair Iddya Karunasagar Introduction to FAO/WHO work on Technical Guidelines on bivalve. Technical Guideline of Codex Code of Practice for Fish and Fishery Products. Iddya Karunasagar Fish & Fishery Office FAO. 9: 00-10:30 h Global bivalve production and issues related to international trade Current status of draft Guidelines : Brian Roughan New Zealand Challenges in the implementation of the programme - Representative from Asia - Representative from Africa - Representative from America

Comments by Ron Lee CEFAS UK CONFERENCE Break 10:30-11:00 How FDA validated their criteria for sewage treatment plant discharges? Gregory Goblick. Shellfish and Aquaculture Policy Branch US FDA. Location: Salón Tronador 11:00-11:30 Parallel session N 3 : Oral presentation Simultaneous translation. Biotoxin & Phytoplankton Location: Salón Volcán A. Day Chair: Begoña Ben Gigirev I- Biotoxin uptake & elimination dynamics 18.- Allan Cembella, Alfred- Wegener-Institut, Germany: Temperature effects on kinetics of paralytic shellfish toxin elimination in the Atlantic surfclam Spisula solidissima (12:00-12:30) LUNCH (12:30-13:45) Bacterial & Virus. Location: Salón Volcán B Day Chair: Jose Barreiro II: Vibrios occurrence & management 14.- Stephen H. Jones. Northeast Center for Vibrio Disease and Ecology, University of New Hampshire, USA Long term of pathogenic VIBRIO SPP. Population in New England U.S. (11:30-12:00) 15. Jose Barreiro, COFEPRIS, México. The Mexican Shellfish Sanitation Program (PMSMB) and the sanitary control of the Vibrio parahaemolyticus. (12:00-12:30) 11:30-19:00 Lunch 12:30-13:45 Break 16: 00-16:30 II- Risk Assessment and Emerging marine biotoxins LUNCH (12:30-13:45) 19.- Nathalie Arnich, ANSES, France: Overview of recent works of ANSES (the French food safety agency) on 16.- Enrico Buenaventura, Health Canada, Canada:

marine biotoxins (lipophilic phycotoxins monitoring in shellfish, ciguatoxins in shark, seawater treatment) (14:00-14:30) 20.- Alison Turnbull, South Australian Research and Development Institute, Australia: Marine biotoxin assessment of Australian wild-caught abalone. (14:30-15:00) 21.- Philip Hess, IFREMER, France: Pinnatoxin-G and other constituents of Vulcanodinium rugosum: analysis, toxicity, distribution in Mediterranean lagoons and accumulation in mussels (Mytilus edulis and Mytilus galloprovincialis) (15:00-15:30) BREAK (16:00-16:30) Risk Management of Vibrio parahaemolyticus in Oysters in Canada from 1997-2014. (14:00-14:30) 17.- Viviana Cachicas, ISP, Chile: Predictive bases of Vibrio parahaemolyticus in shellfish using Air and Water Temperature for Public Health management and production. (14:30 to 15:00) 18.- Moustaf El-Shenawy, National Research Centre. Egypt. Vibrio parahaemolyticus and fecal pollution in zones of bivalve mollusks at the Mediterranean coast of Egypt. (15:00-15:30) 19.- Dominique Hervio-Heath & Michèle Gourmelon, IFREMER, France: A miniaturized MPN real-time PCR method for rapid quantification of total and enteropathogenic Vibrio parahaemolyticus in shellfish. (15:30-16:00) BREAK (16:00-16:30) 20.- Dorothy Jean Mc Coubrey Why has Risk Analysis failed us globally? (16:30-17:00)

JORNADA REGULATORIA JUEVES, Marzo 19 9:00-13:30 Location: Salón Tronador MESA REDONDA SESIÓN REGULATORIA. Moderador : Cristina Martinez ISP /Claudia Rozas Sernapesca Chile EXPERIENCIA CHILENA EN PRODUCCIÓN, CONSUMO Y EXPORTACIÓN Programa de Vigilancia ambiental y seguridad alimentaria en Chile. Lorena Rodriguez. Ministerio de Salud Chile 9:00-11:00

Consumo y vigilancia regional de moluscos. Cristina Hernandez. Jefa de Laboratorio Seremi de Salud Región de Los Lagos. Acuícultura en pequeña escala. Jose Miguel Burgos. Director Sernapesca Chile Experiencia en Codex y Biotoxinas. Cecilia Solis Sernapesca Chile CONFERENCIA 1 SESSIÓN REGULATORIA Break & Posters 11:00-12:00 La ley de modernización de la inocuidad Alimentaria. Desafíos para las exportaciones de alimentos a los EEUU. Julio Salazar. Subdirector de la FDA para América Latina. Gonzalo Ibáñez. Analista Internacional de la FDA. Oficina Santiago de Chile 12:00 A 13:00 Cierre de Sesión Regulatoria: Marisa Caipo, Oficial Regional para Latinoamérica y el Caribe (FAO-RLC) 13: 00-13:15. Lunch 13:15-15:00 CLOSURE ICMSS 2017 proposal Poster Award IAC conclusions of the conference. Location: Salón Tronador 15:00-17:00

Technologic Tour N 2: shellfish farm & processing plant Orizon SA. Km 12.5 camino a Chinquihue Puerto Montt Shellfish Farm and Calbuco area. Friday, March 19 Chair : Gonzalo Diaz Seremi Salud Región de Los Lagos Chile Co-chair : Monica Jara ISP Chile 9:00-15:00 Poster performance in full conference and presented on March 17 and March 19 at Morning Break / Los poster permanecen durante toda la conferencia y son expuestos el día martes 17 y jueves 19 en el café de la mañana. Poster N 1 Poster N 2 BIOTOXINS Evaluation of the possible source of discrepancy in the results obtained after the application of MBA and LC-MS/MS for the analysis of lipophilic toxins Ben-Gigirey, European Union Reference Laboratory for Marine Biotoxins (EURLMB)

A validated PP2A Method - OKATEST-Adapted for determination of lipophilic in combination with LC MS/MS Elena Dominguez Perez ZEULAB S.L.España Poster N 3 Biotoxins monitoring program in Brazil Mathias Schramm, Brazil Poster N 4 Modelling the spatial and temporal dynamics of paralytic shellfish toxins at different scales: implications for research and management Miriam Seguel, Centro Regional de Análisis de Recursos y Medio Ambiente Universidad Austral de Chile Poster N 5 Poster N 6 Poster N 7 Marine toxins: new technologies and their impact in mussel farming. Alexandrium catenella cysts in Southern Chile: An overview of fifteen years of studies National Program of surveillance and control of intoxications and Harmful algae blooms (Red tide) in Chile Myrriam Seguel. Centro Regional de Análisis de Recursos y Medio Ambiente Universidad Austral de Chile. Miriam Seguel. Centro Regional de Análisis de Recursos y Medio Ambiente Universidad Austral de Chile Lorena Delgado ISP Chile Poster N 8 The first well documented event of Paralytic Shellfish Toxins (PST) in blue mussels (Mytilus edulis ) in Sweden BACTERIA & VIRUS Malin Persson, Sweden Poster N 9 Analysis of Vibrio parahamolyticus MAM-7 gene polymorphism by RFLP: Proof of concept of a potential pathogenicity gene marker M J Esquivel Allende, Universidad Mayor Chile

Poster N 10 Poster N 11 Poster N 12 Characterization of Vibrio parahaemolyticus in bivalve molluscs from the coast of Buenos Aires, Argentina using pulsed-field gel electrophoresis (PFGE) Presence of Arcobacter spp. In bivalve molluscs from harvesting areas of the Central Adriatic Sea, Italy Norovirus prevalence in Dutch production area with Class A/B classification. Poster N 13 Norovirus from environment samples associated to outbreaks in Chile 2010-2014 Detection and quantification of genotypes I and II of Norovirus in bivalve Poster N 14 molluscs of the Region of Los Lagos in estival season 2015 Veronica Jurquiza, INIDEP Argentina Francesca Leoni Sezione di Ancona Istituto Zooprofilattico Sperimentale dell Umbria e delle Marche Italy Pol-Hofstad, Irene. Laboratory for Zoonoses and Environmental Microbiology RIVM. Netherland Monica Jara. Food Microbiology ISP Chile Fernanda Carcamo Food Microbiology ISP Chile