CELL BIOLOGY BIOL 309 Fall 2016 Dr. Spilatro Lecture: T & Th 11:00-12:15, Bartlett 362 Lab: T or Th 2:00-5:00, Rickey 204 Text: Alberts et al, Essential Cell Biology, 4 th ed. 2014 TENTATIVE SCHEDULE OF LECTURE TOPICS *** You are expected to bring your book to class *** Lecture Topic Reading assignments Introduction Membrane Structure Chapter 11 Membrane Transport Chapter 12 Cytoskeleton Chapter 17 Quizzes and Exams (Dates are somewhat tentative) Quiz Tues 9/13 Exam Wed 9/28 Energy Generation Chapter 14 Intracellular Transport Chapter 15 Cell Signaling Chapter 16 Quiz Thurs 10/13 Exam Wed 10/26 The Cell Division Cycle Chapter 18 Cellular Communities Chapter 20 Chromosome Structure Chapter 5 & topics on DNA Chapter 6 Quiz Tues 11/8 Exam Wed 11/30 From DNA to Protein Chapter 7 Control of Gene Expression Chapter 8 Quiz Tues 12/6 Gene and Genome Evolution Chapter 9 Final Exam: Wed 12/14 - Noon On-line Class Resources Class resources, including the Powerpoint slides and Question Banks, can be downloaded from the Cell Biology Homepage at http://www.marietta.edu/~spilatrs/biol309/309index.html, which also can be accessed through Moodle. Contact Information & Office Hours Phone: 376-4748 email: spilatrs@marietta.edu Location: Bartlett Hall Rm 173, ext. 4748 Office Hours: Monday 11:00 AM; Thursday 1:00 PM; however, I make every effort to be accessible at other times; feel free to just drop in, if I'm busy, we can schedule another time at which we can meet. 1
TENTATIVE SCHEDULE OF LAB ACTIVITIES Lab manual: Investigations of Cell Biology, Fall 2016 Date Aug 30 Sept 6 Sept13 Sept 20 Sept 27 Oct 4 Oct 11 Laboratory Exercise Introduction / Pipetting & solution making techniques Tissue Homogenization and Protein Measurement Due: Solution calculations Discussion of assigned reading & B16 β-actin project Due: Completed article worksheet Culture of B16 Melanoma Cells Measuring Photosynthetic Electron Transport Fluorescent Staining of β-actin in cultured B16 Cells Due: 1 O articles annotations Friday 10/7 BREAK Oct 18 Electrophoresis & Electroblotting (will begin at 11:00) Oct 25 Nov 1 Nov 8 Nov 15 Nov 22 Nov 29 Dec 6 Immunoblotting of β-actin Due: Introduction section Friday 10/28 Immunoblotting results analysis Histology: tissue resection through embedding Histology, cont., sectioning and mounting Due: Results & Figures Friday 11/18 Histology, cont., staining Histology, cont., photomicroscopy Using Photoshop for image preparation Due: Discussion section Friday 12/9 Disabilities Students who believe that they may need accommodations due to a documented disability should contact the Academic Resource Center (Andrews Hall, Third floor, 376-4700) and the instructor as soon as possible to ensure that such accommodations are implemented in a timely manner. You must meet with the ARC staff to verify your eligibility for any accommodation and for academic assistance. 2
Course Objectives For students planning on attending a graduate or medical program, this course is designed to provide with an understanding of the molecular processes and mechanisms of eukaryotic cells. After successfully completing this course Concepts students should be able to discuss include: characteristics of cell membranes intracellular communication structures and functions of the multicellular interations cytoskeleton DNA and RNA structure and function processes of energy production regulation of gene expression transport and processing of proteins in cells genome evolution Principles of methodologies that students should be able to explain include: tissue culture spectrophotometry protein analysis immunoblot technologies gel electrophoresis histology Elements of the Scientific Methodology that students should be able to apply include: use and understanding of source information critical elements of scientific methodology, including testing of a hypothesis with appropriate controls, interpretation of results, and drawing of conclusions Laboratory techniques that students should be able to apply include: solution preparation pipetting and weighing use of ph meter importance of cleanliness and precise use of spectrophotometer measurements Communication objectives After successfully completing the writing assignments students should be able to: take lab notes adequate for someone else to replicate an experiment and obtain the same results apply proper writing skills and appropriate conventions to a scientific report write purposes and relevant background information for a scientific experiment in a clear and effectively organized style in writing and graphically, present experimental results clearly and unambiguously analyze and draw conclusions about experimental results in context of information from the scientific literature. Student Responsibilities Students have responsibilities for achieving the course objectives. Learning is a process that requires skills and strategies, and you must actively develop those that work best for you. The document Keys to Academic Success describes many ways to improve your learning skills, and you should read and look for new learning strategies that you can apply. In this course the foundation of academic success includes: Attending class Reading the assigned material Being an active participant in the learning process by coming prepared to class, bringing questions about concepts that you do not understand, and answering questions posed during the class period. Completing question banks and working with classmates to understand the concepts Using online resources including self-quizzes, animations, etc 3
Grading and Engagement Policies Your grade will be determined as follows: (points subject to change) Exams 3 x ~100 ~300 points Final exam ~100 Quizzes 4 x ~ 25 ~100 Laboratory grades ~250 ------- ~750 Grading Scale 97-100% = A+ 93-96% = A 90-92% = A- 87-89% = B+ 83-86% = B 80-82% = B- 77-79% = C+ 73-76% = C 70-72% = C- 67-69% = D+ 63-66% = D 60-62% = D- < 60% = F Lab assignments. The first page of the Lab notebook for each lab exercise contains pre-lab questions, which should be completed beforehand and turned in at the beginning of the lab period. The rest of the lab notebook should be turned in at the beginning of the next week s lab, unless instructed otherwise. Answers to post-lab questions (which should be typed), graphed data, images, etc., should also be turned in at the beginning of the next week s lab, unless indicated otherwise. o Prelab questions: 5 points x 5 = 25 pts o Lab notebook/ graphs/images: 10 points x 6 = 60pts o Lab report Annotations and Introduction: 50 pts Results: 25 pts Discussion: 50 pts TurnItIn.com. Written assignments must be turned in to TurnItIn.com. ClassID = 13278028 Password = Biol309-01 Classes missed due to participation in college-sponsored co-curricular events or collegerecognized religious observances are considered excused absences provided appropriate procedures are followed. The student must notify the instructor at the earliest possible time before the absence and arrange to make up missed work as defined by the instructor s syllabus. The co-curricular activity must be a performance, professional meeting, or athletic contest to be considered an excused absence. The religious observance must appear on the College s calendar of religious observances in order to be considered an excused absence. If it does not, an excused absence can be granted only if the student requests special permission from the Dean of the Faculty. An excused absence allows the student to make up exams or quizzes given during the absence, or to reschedule oral presentations. It is the responsibility of the student to get notes from the class and to compensate as much as possible for the absence. It is also the student s responsibility to work with the instructor in determining an appropriate time for make-up assignments. Students must recognize that many classroom and laboratory activities cannot be replicated and that absences may be detrimental to their performance. 4
Notification: I must be notified at least one week in advance if you must miss a class the day of an exam or quiz for an excused absence, at which time an alternative exam time will be arranged. You may not be allowed to makeup an exam if I receive "last minute" notification. Makeup exams and quizzes will not be allowed for unexcused absences. Missed labs: Because of the nature of certain laboratory exercises, even for excused absences make-ups may not be possible and a missed lab will result in a 20 pt deduction. Cell phones and laptop computers should not be on during class or lab periods. Late assignments will be penalized 10% per day and failure to turn in an assignment may result in a failing grade for the semester, at the instructor s discretion. Use of submitted materials: All work produced in this course is considered public and is used for the purposes of teaching and evaluation. This includes the use of your work as a model for future students/courses and the submission of your work to an online plagiarism detection service. Policies also include guidelines in other documents provided for this course (e.g., manuals or handouts) and oral instructions given in class. Extraordinary circumstances. In the case of extraordinary circumstances, the instructor reserves the right to resolve grading issues on an individual basis. Academic Dishonesty Dishonesty within the academic community is a very serious matter, because dishonesty destroys the basic trust necessary for a healthy educational environment. Academic dishonesty is any treatment or representation of work as if one were fully responsible for it, when it is in fact the work of another person. Academic dishonesty includes cheating, plagiarism, theft, or improper manipulation of laboratory or research data or theft of services. A substantiated case of academic dishonesty may result in disciplinary action, including a failing grade on the project, a failing grade in the course, or expulsion from the College. Study Sessions Weekly study sessions will be scheduled. These will be student-organized sessions to review endof-chapter and question-bank questions. These are your best opportunity to review course materials and question bank questions before quizzes and exams. You are expected to have worked on question bank materials before coming to help sessions. 5